Polyps - Science topic
Discrete abnormal tissue masses that protrude into the lumen of the DIGESTIVE TRACT or the RESPIRATORY TRACT. Polyps can be spheroidal, hemispheroidal, or irregular mound-shaped structures attached to the MUCOUS MEMBRANE of the lumen wall either by a stalk, pedunculus, or by a broad base.
Questions related to Polyps
How effective will be endometrial cancer detection from ultrasound images using deep learning? Any kind of suggestion would be highly appreciable
I placed a pulsating soft coral (Xenia umbellata) polyp under the microscope to look at the dinoflagellate symbionts in them. Then I noticed that there are also these darker pink blobs amongst the brown algae and the transparent coral cells.
Does anybody know what these structures are, or what pigments they might be (perhaps coral cells with caretonoids/xanthophylls)?
Am working on a DMH induced colon cancer in mice and i would like to know, could polyps or ACFs be counted through visual macroscopic examination without methylene blue staining ?
Hello, I am a PhD student interested in population genetics of marine invertebrates. Currently I'm working with a bryozoan: Reteporella.
I have been trying to amplify COI (at least) and, although I finally got some bands, after Sanger sequencing, they turned out to be of bacteria, protists, or crustacea (parasits). I'm working with recent samples (collected between 2018-2019), and also some from 2010 (even got bands in these ones, but no sequence). I have been following the conditions suggested in papers dealing with Reteporella and other bryoans, using Platinum Taq (Thermo Fisher) ou Multiplex PCR Master Mix (Qiagen):
- Folmer's LCO1490/HCO2198 universal primers
- 94ºC 3 min; 40x(94ºC 30s, 45ºC 30s, 72ºC 1min); 72ºC 10min
- 95ºC 5 min, 35x(95ºC 40s, 45ºC 45s, 72ºC 1min); 72ºC 8min
I'm aware these temperatures are quite low and prone to amplify inespecific targets. I'll try a gradient PCR 45-55ºC and a touchdown between the same temperatures.Can someone help me, any tips to help me get specific sequences of Reteporella?
Samples were extracted with PureLink extraction kit (Invitrogen), reccomended for "difficult-samples) after completely crushing the sample with a stainless steel pestle (which is passed on ethanol and fire between samples). Only samples that looked "clean" on the surface with polyps at sight (ensuring they were alive when collected) were extracted.
Thanks in advance!
*Attached are eletrophoreses of the PCRs that worked with annealing temperature of 45ºC using the MM Qiagen or Platinum.
We are working with Hydractinia symbiolongicarpus, a hydrozoan cnidarian that forms polyp colonies.
When performing colorimetric In situ hybridizations (ISH), a few hours after adding the Development solution (AP Buffer + NBT/BCIP), we start getting non-specific staining in form of chunks that get stuck to the polyps surface (see pictures). These chunks also appear in Fluorescent ISH (see pictures) , making us think that the problem does not come from the AP buffer development solution but somewhere downstream in the protocol. 'We are using an Urea-based protocol, not using formamide'
We were able to perform colorimetric ISHs that worked before, but something changed through time and we are getting these horrible non-specific staining again. Some real staining can be observed with strong probes beneath all the purple dirty dots, but low-expressed genes are more complicated.
Does anyone know where this problem is coming from? Can anyone give me some tips to avoid obtaining all that non-specific staining in the polyps surface?
In a case of jejunal intussusception secondary to a lipomatous polyp, after laparoscopic reduction of the jejunum it was noted that there was no compromise in bowel vascularity at all. Enterotomy was made and the polyp was extruded out.
To perform a safe polypectomy, should the base of the polyp be stapled or would an endoloop suffice, considering that the lipomatous polyp does not invade the muscle wall? Endoloop is certainly cheaper.
While separating symbionts from live corals using formalin or alcohol, excess mucus is secreted by the Coral Polyp or the colony, which in turn makes difficult to collect the symbionts & also reduces the collection / separation of symbionts.
When studying asexual reproduction of scyphozoan polyps it is rather common to find different authors using the term budding to refer simply to the polyp propagation. In particular, this simplification is used refferring to Aurelia polyps which are able to produce new polyps by means of different asexual modes, some of which differs a lot from a bud. So, should we use this generalization and keep going or should be more careful about what we state?
For isolating DNA from hard corals I need to take polyp from coral fragments. Can you please suggest me how to plug coral polyps from hard coral fragments? Is it possible to pick up from preserved fragments?
Last year in Bonaire (Dutch Caribbean) I encountered this strange colony of the scleractinean coral Siderastrea siderea. It has circular patches of a few cms across with smaller polyps, the smallest in the center. Has anybody seen this as well and what causes this strange growth?
I want to identify some species from soft corals.
primarily i want to know any parts in anatomy and morphological features that knowing of them are necessary for identification, such as:surface coenenchyme, interior coenenchyme, polyp walls, calyces, anthocodiae, tentacles, crown, points , polyp armatures and sclerites
i want to know these names refers for which part of octocorals.
please help me to find a useful source for these
thanks a lot
I am starting my research work on molecular taxonomy of corals. I have a confusion that if we collect coral tissues for DNA extraction is there any chance of contamination of symbiodinium algae with tissue samples? Can anyone suggest how do it possible to collect coral sperm from natural sources?
Does anyone have experience with DNA extraction from polyps? I would like to look at the microbial community and I am unsure how to handle the mixture hard coral and soft tissue. Thanks for any inspiration!
I conducted Alcian blue/nuclear fast red counterstain using the colon tissues of rats and I found that some of samples had a big circular part stained in red as you can see from my profile picture. I guess it might be from the polyp. Does anyone know what this might be? Thank you for your help!
The polyp wasn't removed at the time of the colonoscopy because the patient was on Heparin, and there was a great risk of bleeding. He is a smoker. He is hypothyroid. His CEA and Ca 19.9 were elevated. No masses or ulcers were detected in the Upper GI endoscope. Abdominal Ultrasound was clear. Full body CT scan with and without contrast was clear. It's been 2 months since the colonoscopy was done. What should be done now regarding the polyp?
I am doing video analysis of colonoscopy videos. We are now at a state where we developed a method and a whole system that could be used by a medical doctor. We also tested it on a public available dataset with very good results. The problem is that we need more data and also different diseases (at the moment we only have polyps). Therefore I look for medical experts that are interested in providing their knowledge and using/testing the system.
she has a long history of use of omeprazolo for heartburn without oesophagitis; untill this moment there was a yearly gastroscopy; there are not other polyps in the gastrointestinal tract.
I am currently working on green hydra and would like to collect hydra with as much genetic diversity as possible. Currently I have some hydra from commercial labs, and I am trying to collect some in Texas. Does anyone have green hydra in the lab and can send me some?