Science topic

Polyphenols - Science topic

Polyphenols are a structural class of mainly natural, but also synthetic or semisynthetic, organic chemicals characterized by the presence of large multiples of phenol structural units.
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I want to optimize the parameters of ultrasound-assisted extraction of phytochemicals for my work. I have used amplitudes of 20%, 60% and 100%. I want to check if the Total Polyphenol Content obtained for 100% amplitude is significantly higher than 60% or 20%. Which statistical test should I perform for this purpose? Kindly help, and thanks in advance!
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It is better to be used The PLSR test in the mini tab statistical software ver. 22 or the R statistical software ver. 4.2.0.
Of course, this depends on the nature of the data, its descriptive statistics, and your statistical population. If the statistical population too low, this method does not really work well. It is also to use Monte Carlo analysis maybe useful outcomes.
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Hello, please I need help with a statistical analysis. I don't know what statistical test to use. The study I carried out is as follows: Quantify polyphenols, flavonoids and anthocyanins in addition to antioxidant activity for 2 different concentrations of a fruit. I'm not sure what statistical analysis to perform
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You should use a randomized complete block design, 2×4× reps. You should as much replications as you can to increase prescion of your results
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Hi everyone.
I m actually trying to install a method {COI/T.20/Doc. No 29/Rev.1 2017} for the determination of olive oil polyphenols.
I'm using an HPLC and the result are ok. I mean that the chromatogram is quite well. I can identify every pic and the FFR factor after 7 months is the same.
The problem is that we quantify 100 or 200ppm less than other laboratories and I cannot find the reason.
The preparation of the sample is the same, the only thing that change is the detector of the HPLC. I think is that the problem but I don't know if there is a way to be sure.
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Other thing that can affect is the column. Maybe you need to wash very well the column. I run out with the calibration and samples a quality control samples (with a Concentration know in a spiked blank matrix). You need to validate your method in your laboratory using a matrix blank and spiked with a standard of your analyte in a final known concentration. The parameter that in this case need to validate is recovery, because for a special reason you are obtein half Concentration. Maybe it could be your standar preparation (check very well the calculus), the purity of your solvents, salts or whatever that your need in your method and calculus of the concentration or the pH. Need to check one thing at time, for determinate the mistake in preparation. Also check movil phase and preparation. Good luck!
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How can you say leaves are fresh in terms of its moisture content? Is a 100% moisture content possible? Does it mean that plant leaves that were harvested and subjected right away to IR moisture balance will give us a result of somewhere near 100%?
On the other hand, does "dried leaves" have specific moisture content values to be regarded as "dried" e.g. moisture content should be below a certain percent (10%).
For reference, I am studying Cymbopogon citratus leaves.
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How determine the moisture content in fresh leaves?
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when I measured the total flavonoids and total polyphenols of my 15 day old plants (Vigna unguiculata), I found that the amount of flavonoids is greater than the amount of polyphenols, and to my knowledge flavonoids are polyphenols. so please explain
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While it is not clear how you experimented, there might be a need to check the suitability of your calibration graphs, verify/validate your method before actual measurements, and make use of standards eg phenol and quercetin, use of reagent blanks etc. Sometimes the answer lies in quality control. I am assuming you were using a UV-Vis spectrophotometer. More information from your experiment could have shaded more light.
Nature of sample and sample preparation can also result in such errors
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Hello researchers
Please can you tell me how can we extract alkaloids from plants? Are the extraction of alkaloids same as polyphenols?
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In general, extract into methanol and dry the extract. Take the extract and extract with dichloromethane and water containing 0.1 N HCl. Most alkaloids will extract into the water as their chloride salt. Next, take the aqueous layer and make it basic, around pH 9 or 10, with ammonia. Extract again with dichloromethane. Most alkaloids will be in the DCM layer as their free-base. I use HCl and Ammonia because NH4Cl is volatile and can be removed in the freeze-dryer if the alkaloid of interest remains in the aqueous layer.
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I wanted to extract total RNA in plant fruit tissues which is abundant of polyphenol and polysaccharide in a high yield (about 100-200 ug). Traditional CTAB and TRIzol method were not suitable which performed low 260/230 (<1.6 sometimes even ~0.1-0.5) and low yield. I tried high concentration KCl and PVP-20000 in CTAB extractive solution and added NaCl and Na-citrate while precipitating by 2-propanol, finally after 75% EtOH wash, although 260/230 and 260/280 all come to about 2.20, it still possessed some gel-like things combined with RNA pellet (thick while redissolving by water) and this method has really low yield (about 24 ug for every 800 mg frozen tissues).
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Quan Ma thank you.
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We have synthesized different metal oxide nanoparticles (ZnO,Fe2O3) using aqueous plant extracts as a solvent/reducing agent. XPS and FTIR have revealed the presence of phytochemicals on the metal oxide nanoparticles. Can anyone suggest an analytical technique to to identify the phytochemical incorporated in the metal oxide during the synthesis? Our primary inference is that it may be some of the polyphenols present in the plant extract. Can ESIMS be used or are there any other techniques?
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Thank you,for your generous answers.
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Technically, it is not possible as flavonoids are part of polyphenols.
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One of the reasons is that total phenols are frequently measured using the Folin-Ciocalteu reagent/methods, which is very susceptible to interference from non-phenolic substances and thus yields results that are too high.
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i am currently looking for methods to detect a polyphenol compound in packaged grape juice with the highest sensitivity. i am however worried that the additives in juice may interfere with my detection assay. it may help to know how they react with the polyphenols so that i can perform that assay without any flaws. can you please state the ways in which these food additives might interfere with my detection assays?
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Commenting for better reach
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Hello,
I'm attempting an LC-MS analysis for a plant methanolic extract for the sake of polyphenol and terpenes quantification. I'm following a protocol used by Nivea M. et al., (2019) in which they used Ribitol as an internal standard.
I was wondering if I can substitute Ribitol with another standard and if I can then what are the possible choices I can use?
Thank you
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Hi.
I see that many papers are analyzed using external standard methods.
Are you concerned about the recovery rate from the analyte?
I think you have a few compounds to choose from following articles.
baicalin
catechin and kaempferol.
quercetin
biochanin A
Anyway, of the polyphenols that would not be in your analyte, you can use those that are relatively close in nature and available in high purity. I believe you can solve this problem by selecting the appropriate one that is not contained in the analyte.
And I believe the topic someone else posted earlier may be helpful...
Good luck!
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I am currently doing a research on targeted polyphenol profiling of one plant from different locations. Together with this i am going to test their antioxidant activities as well. I am confused if I should apply the biological replicates here because of the recommendations from the metabolomics standards initiative. Is this applicable to my study? I am concerned with the ampunt of samples because it might take too much time and resources since i have 5 locations and i will be testing both fresh,oven dried and air dried samples so a total of 3 samples per location. If i do this is 3 biological replicates, it’ll be 9 samples per location.
Anyways regardless, I will still be analysing each samples in 3 trials.
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Maintaining biological replicates will definitely reduce the sampling error and influence of environmental and edaphic factors and even sample collection will have seasonal variations and once confirmed a lot these screening and can fix the season'location and number of replicates.
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After extracting my industrial fruit waste (dried 50g in 100ml distilled water) and measuring its TPC. I went straight into assessing its antibacterial activity. My extract is in liquid form (no rotary evaporation/lyophilization/spray drying). After quick well diffusion tests, I attempted determination of MIC (microbroth dilution method) with Mueller-Hinton broth in U-bottom 96-well microplates.
I then came to realize that I don't exactly know the concentration of my extract. Thus, my question is could I use the mg GAE/ml as the concentration? or is my concentration 50g/100ml i.e. 500mg/ml? or should I dry in the oven (100-120oC) overnight 1ml of my extract and calculate the weight difference per 1ml as my concentration?
Thanks
Edit for anyone interested: After several adjustments, the antibacterial assessment will follow with considering the waste in its "crude" extract state and assessing its MBC/MIC ratio with the literature.
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Your question is tricky because you started the work without knowing the concentration of your extract. You can not say your conc. is 50g/100mL or 0.5g/mL because 50g of dry fruit waste did not completely dissolve in 100mL of solvent and of course can not determine MIC if you do not have knowledge on the conc. of the starting material.
My humble advise is "re-do the assay, but this time after drying the fruit waste, grind and macerate (at least 48hours). Then filter and concentrate using rotary evaporation to obtain an extract prepared from fruit waste". From where you can prepare a 25mg/mL stock from which you can prepare you test concentrations.
Good luck..
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Hi guys,
I need some advice over here.
Im trying to do a liposomal encapsulation via microfluidics of a natural extract diluted in etanol, it is rich in polyphenols. Im using Oleic Acid + Cholesterol + tween 20 for the lipid layer. During the process in getting a precipitate and very few microcapsules.
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Based on our previous experience, faty acid is not suitable for this process. You can use phospholipid instead of oleaic acid, which would be preferable if you avoid Tween/Span.
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I have some columns for my Thermo Scientific GC-MS:
1) DB-WAX
2) DB-1
c) DB-17HT
d) VF-1MS
e) TR-FAME
f) TG-5MS
g) TG-WAXMS A
In my opinion, for polyphenols as flavonoid (and their glicosides) and phenolic acids (present in vegetable, grains, food analysis), I would prefere DB-1, VF-1MS and TG-5MS.
Your opinion will be valuable for me...a lot!
I am using TG-5MS. but I expected better results than the ones I am having...
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Use DB-1 or DB-5MS
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is normal or less sugar is better to reduce acrylamide content in potato chips? and why??
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يتكون الاكريل الامايد من السكر ومن النشا ولكن كلما قل السكر الحر عند قلي البطاطا افضل
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I did a qualitative screening of 2 plants extracts. And both plants showed a negative result for polyphenol but positive for flavonoids. I know flavonoids are a class of polyphenols so I'm a bit confused on how to interpret that. And take into account that the quantitative screening for TPC (total phenolic content) shows results.
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Hi RNA experts,
I am trying to extract RNA from my blueberry fruit samples. They have been stored in -20 C for one or two months and then they were moved to -80 C. I used the Qiagen RNeasy Plant Mini Kit for extraction. The same lab/method/environment used for RNA extraction in other plant materials was used but I failed to extract RNA for these blueberry samples.
From QC, I got:
1. "too low" or close to 10.0 results (very low) from Qubit BR assays.
2. no visible bands from agarose gel electrophoresis.
3. 0.7-1.9 260/280 ratios from nanodrop (extensive variation).
What do you think the problem could be?
Could the high water content of the fruit be causing poor extraction?
Could storage temperature be the problem? Is it okay to store the samples before RNA extraction at -20 C?
Could the polyphenolics and polysaccharides be hindering extraction?
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Thank you everyone for your help. I used the Sigma Aldrich Spectrum RNA extraction kit and I got perfectly good extractions. I used the exact same samples.
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What are the appropriate conditions for making the reaction take place between FeCl3 & suitable plant to reduce Fe+3 to Fe+2 (Apart from forming iron oxide), without forming IronPolyphenols?
where the difficulty is due to chelation process that occurs by polyphenols found in the plant.
so how can i overcome the problem?
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Dear Omar Yousef thank you for sharing this very interesting technical question with the RG community. Unfortunately you did not really specify the goal of your experients. Is the final goal just the reduction of Fe3+ to Fe2+ or the formation of some sort of iron-containing nanoparticles?
As for the prevention of the formation of iron-polyphenol complexes, please have a look at the following potentially useful PhD thesis in which this problem has been studied in detail:
Prevention of Iron-Polyphenol Complex Formation in Iron Fortified Tea
(please see attached pdf file)
Also please check this relevant article:
Prevention of iron-polyphenol complex formation by chelation in black tea
This paper is freely available as public full text on RG so that you can download it as pdf file.
Good luck with your work and please stay safe and healthy! With best wishes, Frank Edelmann
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Dear colleagues, In recent years a lot of research groups started to use prediction tools such as metabolomic circuit, QSAR, and molecular docking as additional tools to gain time and reagents. Some articles described the polyphenol and flavonoid contents without determinating the chemical composition of plant extracts, I would like to know if there is a tool that can be used to predict antioxidant activity from the TPC and TFC, if yes how it can be done and is it a reliable tool ?
best regards.
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We are looking for high purity brown algae polyphenol (phlorotannins) samples. I am currently based in China. If you have any information about where could I purchase samples. Please let me know. Thanks.
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Weihao Meng Always welcome!
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We observed the low antioxydant activity of our extract whereas the level of total flavonoids and total polyphenols are high.
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I ran my methanolic extracts (flavonoids compounds) over silica gel, then no separation took place due to the complexity of the mixture. I collected all the test tubes fraction together and I removed the solvent. Consequently, the first color was white turned yellow! While, I didn't separate any fraction and all the fractions were concentrated together!
How can we explain the change of colors, whereas we didn't make any isolation?
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As we know Like dissolves Like; polar analytes are dissolved in polar solvents.
I noticed in an article that they use the soxhlet apparatus with a temperature in the range of 70-80° to recover methoxyflavoinds (polar class of compounds)!
I'm just questioning if the temperature might plays the role to extract polar compounds even with apolar solvent?
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Kindly see also the following useful RG link:
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Are there other methods besides the Folin-Ciocalteu method and Prussian blue?
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Dear Katarzyna, many thanks for sharing this interesting technical question with the RG community. Concerning your question "Are there other methods besides the Folin-Ciocalteu method and Prussian blue?" please have a look at the following article in which the "ferrous tartrate method" is used:
Extraction of total phenolic and flavonoids from edible wild and cultivated medicinal mushrooms as affected by different solvents
This article is available as public full text on RG. Thus you can freely download it as pdf file.
Good luck with your research and best wishes!
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I am a PhD student from Nigeria and am working on nutritional interventions against heavy metal toxicities. I enriched polyphenols from some plant extracts and i wish to characterize the polyphenol contents of these extracts using LC/MS or other appropriate technologies. Please can anyone refer any laboratory that accepts international samples for such analysis? I am also open to collaborate with scientist that are expert in doing such types of analysis. Thanks
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Dear Francis, apparently it helps reading you original question carefully. You can e.g. search the internet for terms like "LC/MS service in Nigeria". This provided for example the following address in Lagos:
SGA Analytical Chemistry
I also suggest that you search the various departments of your own institution, the University of Port Harcourt, for researchers who perhaps have LC/MS facilities, e.g. the Department of Pharmaceutics and Pharmaceutical Technology. See e.g. the following potentially useful article:
Gas Chromatography-Mass Spectroscopic (GC-MS) Analysis of n-Hexane Extract of Lentinus tuber-regium (Fr) Fr (Polyporaceae) Syn Pleurotus tuber regium Fr sclerotia
(please see attached pdf file)
I hope this helps. Good luck with your research!
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I cannot find sources which gives a thorough explanation of PCA and how to assign the principal components 1 & 2 including their computations. Say for example, my study will explore polyphenol profiles of a certain plant from different geographical area and I will test their antioxidant activity too and analyze them using biochemometric approach. Which variables should be included in principal components? I will also integrate data from this PCA to construct my OPLS DA.
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Hi Duri,
I organized some information about PCA and PCR on:
The original is in Portuguese but can be translated by Google Translator:
Best Regards,
Markos
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Does anyone know the method for the determination of polyphenol content in addition to the Folin-Ciocalteu method and Prussian blue?
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I am preparing phytosome which is a drug delivery system using phosphotidycholine dissolved in DCM and polyphenols dissolved in water then homogenize....The results I get for zeta potential is negative yet am trying to target positive zeta potential but the size I am getting is below 250nm...When I introduce chitosan in the preparation I am getting A zeta potential above +30 mV but the size is above 1000nm. Is there anyone who can help me know where i am going long ...or is there anyone who can help me know an alternative i can use beside chitosan?
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What is the best method for Polyphenol oxidase in grapes. I had already try following method:
Procedure
Adjust the spectrophotometer to 280 nm and 25°C. Pipette into each cuvette as follows:
0.5 M Phosphate buffer, pH 6.5-1.0 ml
0.001 M L-Tyrosine-1.0 ml
Reagent grade water-0.9 ml
Oxygenate this reaction mixture by bubbling oxygen into cuvettes
through a capillary tube for 4-5 minutes. Transfer cuvettes to the spectrophotometer and record A280 for 4-5 minutes to achieve temperature equilibration and to establish blank rate, if any. Add 0.1 ml of appropriately diluted enzyme and record A280 for 10-12 minutes. Determine ΔA280 from the linear portion of the curve. A non-linear "lag" of 2-3 minutes can be expected.
But i got negative reading and not significant change.
Please suggest me the method.
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you can also use this method.
The reaction mixture contained 0.8 ml of 100 mM potassium phosphate buffer (pH 6.0), 50 μl of aerated 5 mM (+)-catechin, dissolved in assay buffer 0.25 ml enzyme extract. The mixture was incubated at 30ºC for 30 minutes. The reaction was stopped by adding 0.4 ml of 2 N HClO4. Finally, the stopped reaction will be centrifuged at 5000 rpm for 10min. The absorbance of the oxidized catechin will be read at 395nm.
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Hi, I am checking the antioxidant activity of polyphenols using the DPPH reagent. I'm taking a 0.1mM reagent. I tried two 3 things. I'm using ethanol as a solvent for my extract.
1. I made DPPH SOLUTION USING ethanol as my extracting solvent was ethanol and checked the absorbance but my standard values are coming negative while the test sample is giving +ve.
2. I also tried methanol. Made DPPH solution using methanol. here for control, I took DPPH standard solution + ethanol (s my extracting solvent was ethanol ) and test samples as meth DPPH soln with the extract. still, the absorbance values are either less or -ve.
I have tried both methods on three different instruments. Please suggest something to do?
thanks in advance!
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1. Calibrate the UV-vis
2. Change the cuvette, better to use fresh quartz cuvette
3. Go through any of the above-mentioned protocols.
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I am trying to make nanoparticles using tannic acid and I am using Triton X-100 as the surfactant (not for stabilizing nanoparticles), which is added to tannic acid prior to the synthesis reaction. Can Triton X-100 react with tannic acid or change its structure or vice versa?
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Dear Sina,
this is a very interesting technical question. As an inorganic chemist I'm absolutely no specialist in this field of research. However, I just came across a potentially useful article which might help you in your analysis. In this paper the interaction between tannic acid and several non-ionic surfactants (including Triton X-100) has been investgated in detail:
Effects of non-ionic surfactants on the interactions between cellulases and tannic acid: A model system for cellulase–poly-phenol interactions
Unfortunately this paper has not been posted as public full text on RG. However, five of the authors have RG profiles, so that it should be easily possible to request the full text directly from one of the authors via RG.
Good luck with your research!
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I extracted DNA from some kombucha products with a manual protocol, with one final step using phenol, for 16S and ITS sequencing.
However, there is a problem for the majority of my samples in PCR step. Apparently, there is/are some inhibitors in my samples that impede PCR. My samples pH is around 3 and they contain polyphenols.
Did anyone have any similar experience or any suggestion?
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Thank you all for your kind replies.
I purified my samples with Nucleospin tissue kit and it worked out.
Hope this can help someone else.
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I need to determine a polyphenol profile per HPLC from yacon flour but I did not find a suitable protocol as there are too many of them. Can somebody help me?
Thanks!
Romina.
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I prepared you a protocol that may help you in your work, you will find it in attached.
I send you also the article source.
Best wishes,
Sabri
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How is yeast being used in encapsulation of polyphenols? Plasmolysed or intact cells?
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Dear Imen Laib thank you for posting this very important technical question which is of significant current interest. In addition to the relevant references suggested by Chinaza Godswill Awuchi and Kiprotich Kiptum please also have a look at the following useful review article:
Encapsulation of polyphenols – a review
This review article has been published Open Access and is freely available as pdf file (see attachment).
To give you a possible answer to the second part of your question (Plasmolysed or intact cells?) I suggest that you go through the following very interesting article reporting the mircoencapsulation of curcumin (as a typical polyphenol) in yeast cells. As you certainly know, curcumin has a large variety of health benefits. However, free curcumin has a low bioavailability. Thus one promising approach to improve its bioavailability is microencapsulation in yeast cells:
Microencapsulation of curcumin in cells of Saccharomyces cerevisiae
This paper has been posted by the authors as public full text on RG. Thus it can be freely downloaded as pdf file. As you will see, the process rescribed here includes plasmolysis of the yeast cells.
I hope this helps answering your question. Good luck with your research and best wishes, Frank Edelmann
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We are trying to estimate curcumin content in Turmeric genotypes. Rhizomes are cut in pieces and oven dried at 60 o C. Certain genotypes retained the original colour on cut surfaces and others changed to brown to dark brown on cut surfaces. What is the reason? Does polyphenol play a role ? What about other factors? We are sure that it is not due to excessive heat, as we have seen this even under lower temperatures.
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Sometimes infected zones typically appear as dull brown and dark.
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Flavonoids are a group of plant secondary metabolites thought to offer health assistances through cell signaling pathways and antioxidant effects. These molecules are found in a variety of fruits, barks and vegetables. Flavonoids are polyphenolic molecules containing 15 carbon atoms and are soluble in water. How I can i isolate and separate polar flavonoids from methanolic extract easily.
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Silica gel chromatography is the main method to isolate or identify flavonoids. It is applied to isolate low or medium polar constituents. Reversed phase silica gel (e.g. reversed phase C18 silica gel) is commonly used to isolate flavonoid glycosides.
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What is the reason explaining that the higher percentage contents of total polyphenols and total flavonoids significantly in dried mint leaves compared to the fresh ones?
Why does drying increase the biological activities (antioxidant and anti-inflammation) of total polyphenols and total flavonoids in plant leaf extract?
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Sample: plant seeds, and ethanolic extraction assisted by ultrasonic used for our samples. Both identifying and quantifying are being targeted.
We have BSTFA but I don't know the protocol of these process, and it's possible to use only BSTFA or I should mix derivative reagents. I need to know all the conditions of the process and if any other chemicals or reagents needed.
thanks in advance
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It would greatly assist if you could provide more details regarding the sample(s), sample preparation and the identity of the analytes (polyphenols). Also are you wanting to identify or quantify or both?
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In adding DHA to a health supplement, I want to know which polyphenols are best to protect against oxidation in vivo. Have you found commercially available sources and if so, can you please share this information with me? We are looking at humans and companion animals. Thank you for all you do:)
Bob Rager
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Is there any way to construct a full set of database of natural polyphenolic compounds for molecular docking. Preparing the ligands one by one is time consuming? Can anyone share a easy way to do so?
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Assalamualaikum vai, I visited the website of phenol-explorer and found a total of 501 food polyphenols. Preparing all those one by one in pdbqt format for docking is very time consuming. Is there any software or procedure to download all the compounds from a library like drugbank or zinc in a readily dockable format?
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I have an animal RNA extraction kit (silica column-based kit), how to adapt it for plant use? Knowing that the kits for plants normally contain two types of columns (one for preliminary filtration and the silica column) while kits for animals contain only one type of column (silica column). Other than centrifugation, how will I be able to remove debris and polyphenols?
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Can you use a filter prior to the silica column? Look up the size for the preliminary filtration and you may be able to just use a filter instead. I do this when trying to remove cell debris from my supernatant. Be sure the filter you use wont shred your RNA though.
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So I understand that essential oils are formed by steam distillation, and contain volatile, usually aromatic, compounds.
Extracts are formed by immersion of plant matter in a solvent, and are usually reported to contain compounds such as polyphenols, anthraquinones, flavonoids and the like.
Practically every report I have read does not mention whether obtained extracts contain the volatile compounds found in essential oils though. Will there be volatile essential oil compounds in extracts, or can they ONLY be taken out of plant matter by steam distillation?
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essential oil and volatiles are similar, but not the same! Plants, extracts (except Citrus sp peel oil) by definition DO NOT contain essential oils but volatiles! this is totaly technical description. only distillations are accepted for the production of essential oils.. Also CO2 extracts are NOT essential oils even they may contain volatile compounds!!!!
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I'm exploring the world of emulsions, but I'm not sure if it is the correct way to solve my problem.
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After searching among literature for months, I am not still able to choose a microalga strain for my PhD project. The idea is to extract as many as possible valuable compounds (such as pigments, PUFAs, polyphenols, phytosterols, vitamins) from the same microalgal biomass.
To do this, I need a strain that is being already studied and optimal conditions growth for bioproducts target are known. Also, I am not sure that selecting strains already on the market (maybe lipids or biofuels market) could be a good idea.
Which strain do you suggest?
I've found a promising strain (Stichococcus sp. KMMCC 365 but it's impossible to find to buy)
Thanks to everyone that will spend sometimes to reply me
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For doctoral research, I don't think it would be a good idea to select any strain which has already been explored. I would suggest exploring new strains from the natural pools of that specific area where you are pursuing your doctoral research.
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Hello everyone, I have tried to correlate the results of the following analyzes: (total polyphenols, total flavonoids, condensed tannins and flavonols) with the IC50 of the DPPH antioxidant activity test. can you please help me interpret these results? I don't understand why the correlation is weak for polyphenols and flavonoids. My samples are the leaves and stems of a plant extracted by 2 different extraction methods L: Leaf S: rod L1: Leaf (2nd method) S1: Stem (2nd method)
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We are reviewing whether antioxidant and anti-inflammatory polyphenols really be developed as supplements or drugs for anti-aging and aging health-related illness? Any possible or not suggestions?
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yes
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Hello, Please I need help with a statistical analysis. I don't know which statistical test to use. The study I did is as follows : an extraction by maceration of the leaves and stems of a plant and an ultrasonic assisted extraction of the leaves and stems as well. I then performed the following tests in triplicate (polyphenols, flavonoids, flavonols, condensed tannins and DPPH).
I want to make the following comparisons: - leaves with stems - Leaves (maceration) with leaves (sonication) - stems (maceration) with stems (sonication)
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If you want to look at the statistical difference between groups when all the work is done, you can do One-Way Anova if you have enough examples.
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Especially, we refer to the presence of molecular phenol in OMWW as a general problem. "The fraction obtained at the end of the batch membrane operation appeared to be rich in polyphenols and should be processed for their recovery in regards to the negative impacts of phenol".
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Greetings! I'm trying to develop a quick routine method (quality control) to determine polyphenols in hydroalcoholic extracts of Moringa oleifera seeds. However, the test results reflects considerably high values (1,71 gr/L eq. of Gallic Acid).
The extract was made in a 1:10 solute-solvent ratio, using 70% Ethanol. It was filtered, diluted in water and centrifuged at 10.000 rpm. The proteins were apparently extracted and the levels of sugars and vitamin C in Moringa seeds are very low.
It's possible that there is still some interference? Maybe the oleic acid? I appreciate your help.
Best Regards! Arturo Torres
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Dear all, I'm conducting the revisions of some of my manuscript, with is the impact of the combination of Aspirin and a fruit juice in colorectal cancer cells. Two of the reviewers have asked to calculate the substance interaction index, but the juice is not a pure substance, but a mixture of thousands of components (we only quantified some polyphenols). Does it make sense to calculate an interaction index? Thanks in advance for your help.
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Hi,
Reviewers seemed to have asked a general question, but you need to experimentally observed more clearly into the physicochemical/interfacial phenomena of the object of your study. For instance, aspirin is a poorly water-soluble drug with a pKa of 3.5. Generally, polyphenol molecules have both hydrophobic moieties and hydrophilic hydroxyl groups. The interaction between polyphenols and aspirin probably greatly depend on your assayed conditions, which would ultimately influence your evaluated index (ex vivo).
Good luck.
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In order to measure the polyphenols in broccoli, we prepared a reagent for measurement using the following procedure.
1. Extract the crushed plant with 70% methanol.
2. Mix 1 ml of the extract with 5 ml of 7.5% sodium carbonate and leave for 3 to 8 minutes.
3. Afterwards, 4 ml of 10% Folin-Ciocalteu reagent was mixed to make a total of 10 ml of measurement solution.
Immediately after mixing the Folin-Ciocalteu reagent, the mixed solution becomes cloudy. The color of the standard is clear.
What is the possible cause?
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try to change the solvent and the procedure
100 μl of each of the extract / standard were mixed with 500 μl of water and then with 100 μl of Folin-Ciocalteu reagent and allowed to stand for 6 minutes. Then 1ml of 7% sodium carbonate and 500 μl of distilled water was added to the reaction mixture. The absorbance was recorded after 60 minutes at 760 nm spectrometrically
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I am currently preparing to isolate polyphenolics (Hydrolysable tannins) from aqueous extract of a plant. I want to set up a small sephadex column to guide the major isolation on a laerge column.
My column is 20cm high and 40ml. How do i pack it? Is it okay to dissolve my extract in 50% Ethanol, even though I am thinking of starting with 100% Ethanol, followed by 10%, 30, 50, 70% Methanol?
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You have to try. I separate the tannins on HPLC. But not all of them. Unfortunately, sometimes you have to use trial and error.
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Fermentation cause to lowers  antinutritional factors in cocoa, I have a question regarding this issue, in fermentation polyphenol are consumed in tanning reaction with albumin protein which cause to insoluble the polyphenol then reduce the bitterness , when polyphenol is insoluble how it can reduced.
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Boiling or steaming increase anti nutrients because as heat is energy,the useful functional groups like phenolic or others in carotenoids,the energy level in the last orbits of their atoms as more negetive are not stable somewhat breaks up losing its original nature .
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I proposed in March 2020, in my article ˝Immune system and COVID 19˝ (DOI: 10.13140/RG.2.2.21811.99366) ˝factors that can contribute to the better immune system functionality are healthy balanced diet (with addition of vitamins and trace elements if necessary), moderate physical exercise, ingestion of polyphenols and natural antivirals, protection of nasal and oropharyngeal mucosa with mucosal care and protection products, and smoking cessation.˝
Well, today, some colleagues agree that protection of nasal mucosa can help in prevention and treatment of COVID-19 disease. I am glad to help!
Example:
Do you have any similar data or experience to share?
Thank you!
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Thank u for any answers about my questions!
I have planned to study the polymeric polyphenols from plants degraded by human gut microbiota,and the fermention composition of fecal slurry with polymeric polyphenols was very complex,a valid extraction method should be used to obtain these degradation products.
May i know what is the best way for the extraction of fecal metabolites?
Ethyl acetate?
methanol/acidified water (0.1% formic acid) (80:20 v/v)?
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Acturally,i am a curious about what composition in the medium is good for the polyphenols In vitro fermentation by human colonic microbiota?
· Peptone-1.0 g
· Yeast extract- 2.0 g
· NaCl- 0.05 g
· K2HPO4- 0.02 g
· KH2PO4- 0.02 g
· MgSO4- 0.005 g
· CaCl2- 0.005 g
· NaHCO3- 1.0 g
· Hemin- 0.01 g
· Cysteine-HCl- 0.23 g
· Bile salt- 0.25 g
· Resazurin- 0.5 g
· Tween 80- 1.0 mL
· Glucose 5g
· vitamin K1- 5 μL into 500 mL distilled water and adjusting pH to 7.0 using 0.1 M HCl solution.
This composition of growth medium is enough for the fermentation?
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Why are polyphenols acylated? and what methods are used for polyphenols acylation??
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Possess functional groups like O, CH3, OCH3 and C=O
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I started by extracting roots samples (8 samples) with boiling Methanol, after evaporation and drying the yield was about 10g (for each sample) of solid material, now for the purification of polyphenols what of these procedures should I use (SPE C18 column, sephadex LH-20 column chromatography, TLC, and HPLC) and what is the correct order of them?
Can I use silica gel (60-120 mesh) as an alternative to sephadex LH-20?
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Dear Aws Bunyan Polyphenols as phytochemicals have gained significant importance owing to several associated health benefits with regard to lifestyle diseases and oxidative stress. To date, the development of a single standard method for efficient and rapid extraction of polyphenols from plant matrices has remained a challenge due to the inherent limitations of various conventional extraction methods. The exploitation of polyphenols as bioactive compounds at various commercial levels has motivated scientists to explore more eco‐friendly, efficient, and cost‐effective extraction techniques, based on a green extraction approach. The following modern green extraction techniques—supercritical fluid extraction, ultrasound‐assisted extraction, microwave‐assisted extraction, pressurized liquid extraction, and pressurized hot water extraction—as alternatives to conventional extraction methods for polyphenol extraction. These techniques are proving to be promising for the extraction of thermolabile phenolic compounds due to their advantages over conventional, time‐consuming, and laborious extraction techniques, such as reduced solvent use and time and energy consumption and higher recovery rates with lower operational costs. The growing interest in plant‐derived polyphenols prompts continual search for green and economically feasible modern extraction techniques. Modern green extraction techniques represent promising approaches by virtue of overcoming current limitations to the exploitation of polyphenols as bioactive compounds to explore their wide‐reaching applications on an industrial scale and in emerging global markets. Future research is needed in order to remove the technical barriers to scale‐up the processes for industrial needs by increasing our understanding and improving the design of modern extraction operations.
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I am working on ultrasound assisted extraction on ginger, the solvents type included in my study were ethanol, methanol and distilled water, what explanation is there for i get highest TPC and TFC (phenolic and flavonoid content) using ethanol as solvents. as i found out that methanol should have higher dissolution.
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The polyphenols on ginger you are working with might capable of forming hydrogen bonds with ethanol, the group of polyphenols matches with the polarity of the ethanol. The polyphenols with such property usually have a short carbon chain which has a high solubility in alcohol. Their tendency to be solubilized into ethanol govern by the thermodynamic nature of the compounds.
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I usually use the Bradford Assay, but have had problems (high values... I used 1: 100 dilution, Should I dilute it more?). These serum samples are from rats that have consumed CD or HFD, and a polyphenolic extract supplementation.
What do you recommend me to do?
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I also think that the BCA assay is more reliable, as Yunus Shukor mentioned already. However, be aware that all these assays are semiquantitative in a strict sense. It very depends on the question you want to answer.
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I used Discovery studio for molecular docking, I used several polyphenolic compounds, and the compounds that showed the highest binding energy contain several unfavorable bumps. Is this can affect binding energy? If can, how does it affect?
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Molecular docking usually predicts the pose of certain compounds in a target protein pocket. For good docking calculation, it is expected that the predicted protein-ligand complex should contain no intermolecular steric clash (or bump). For binding energy, molecular dynamics simulation is therefore suggested, considering MM-PBSA or MM-GBSA binding energy calculation.
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I'm studying on the long term effect of polyphenols on gene expression in indigenous chickens in Zimbabwe. Polyphenols are known to be epigenetic (do not modify genes but just affect expression).
Which is the best part of the chicken for DNA extraction?
What can be the best indicators of gene expression which i can record?
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I think you can do the gene expression analysis by taking target genes as glutathione peroxidase and superoxide dismutase. You can compare the expression levels comparing to a house keeping gene such as GAPDH
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I do the extraction from powders with various solvents (ethanol, acetone) and I would like to express the result per liter. do I have to change the calculation formula?
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I want to express de content of polyphenols per liter because I use the extract as such, and I think it is more correctly to express it per litre
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Dear colleagues, I need specialists’ suggestions who deal with the extraction of polyphenols.
I am wondering whether there is a green and cost-efficient extraction technique of ferulic acid from wheat and rye bran?
Based on my results and the data obtained by other researchers I know that ferulic acid is the main phenolic acids representative found in cereals. However, this hydroxycinnamic acid derivative is extremely integrated into cell-walls of plant material and classified as non-extractable polyphenol. The recovery of ferulic acid makes up 1%.
I did several experiments with different bran samples using conventional extractions; however, the results leave something to be desired.
I also conducted hydrolysis of my samples with NaOH and HCl, the recovery is much better, though this approach led to the destruction of acid, base, and thermolabile compounds.
Thank you very much for your valuable suggestions and comments on this matter!
Yours sincerely,
Vitali
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According to my experience, The use of Ultrasound-assisted extraction as an extraction method and the methanol + KOH (5%) as an extracting solvent may give a satisfy result.
All the best.
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In order to quantify the polyphenol consumption for a given population, how can we make it ?
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Hi.
  • This is an interesting question. There are two ways to do this work. First, you can conduct a literature search to find those papers that formerly did this issue for their projects. Some systematic reviews and/or clinical trials can strongly support you for the quantification of polyphenols. The following study is a perfect example. https://pubmed.ncbi.nlm.nih.gov/22968335/
  • The second option is to use PhenolExplorer database information. The developers of this excellent database provide so much data on the exact content of food polyphenols and associated parameters. You can reach it through http://phenol-explorer.eu/
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Hi,
There are lots of studies on probiotics and dietary polyphenols to modulate the gut microbioto. Please provide few specific mechanisms through which dietary polyphenols regulate the gut microbiome.
Thank you
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Polyphenols are produced by plants to protect themselves from UV and reactive oxygen species. Polyphenols on health has often been explained by their anti-oxidant properties.Those large and complex polyphenols are not digested by our own enzymes and proceed to the colon where they can be broken down into smaller active metabolites by bacteria, making them easier to absorb in gut.
Mechanisms of action of dietary polyphenols varies in Gram positive and Gram-negative bacteria due to changes in cell membrane structure. Polyphenols have ability to bind bacterial cell membranes in a concentration dependent manner, therefore altering functional aspects of membrane and thus preventing their growth.
Polyphenols have prebiotic-like effect by promoting the growth of certain bacteria such as Bifidobacterium, Bacteroides and Lactobacillus.
Polyphenols do not just raise the quantity of good bacteria but also inhibit the proliferation of many pathogens. For example, polyphenols from tea and wine canprevent the growth of Helicobacter pylori and polyphenols extracted from olive oil can prevent damage caused by the bacteria.
Polyphenols can also directly mediate inflammation by interacting and blocking TLR4, thus reducing the production of inflammatory mediators like IL-1b, IL-6 and TNFa.
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I conducted experiments of adsorption/desorption of a mixture of compounds on macro porous resin in fixed-bed continuous-flow and I calculated the experimental and the theoretical stoichiometric times. What is the meaning of the stoichiometric time? it refers to what exactly?
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I apologize for my incorrectly interpreting your question; I send you the link to a paper, hoping it will help you:
-Adsorption Basics: Part 1
By Alan Gabelman, P.E.
American Institute of Chemical Engineers (AIChE) (2017)
Good work and my best regards, Pierluigi Traverso.
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HPLC method for the simultaneous detection of tocopherols, flavonoids and polyphenols in vegetable oil.
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Yes, it is possible follow the Article mention by @Abdrerrahmane
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what is the mode of action of polyphenols and flavonoids as antibacterial agents.
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Phenolic compounds (such as polyphenols and flavonoids) are hydrogen donors capable of directly scavenging free radicals and reducing oxidative damage, which makes them potent antioxidants. In addition to the antioxidant activity, phenolic compounds act as antimicrobial agents via several mechanisms including the disruption of microbial membranes. Because of the differences in the cell wall structures between Gram-positive and Gram-negative bacteria, This explains why the Gram-negative bacteria are generally more resistant to plant extracts (containing phenolic compounds) than the Gram-positive bacteria. Gram-negative bacteria have an extra hydrophilic outer membrane that functions as a preventive barrier against hydrophobic compounds and inhibits the accumulation of phenolic compounds in the target cell membrane.
For more information, DOI: 10.4103/epj.epj_44_18
Antioxidant and antibacterial properties of anise (Pimpinella anisum L.) .
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Hello everyone,
The crud extracts of peganum harmala seeds contains alkaloids and polyphenolic compounds and i want to do the HPLC , LC-MS or RMN without extracting them, can you give me the solution please? can i put the alkaloids and polyphenols standars together or not ?
Thanks
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To obtain the concentration of polyphenols I made a calibration curve of GA. The equation was y = 0,0351x + 0,0098. I solved 10 gram of green tea in 200 ml of water for 2h at 50°C. Afterwards I took 40 ml of this extract and diluted it to 200 ml to color the textile for 45 min. Every 15 min I took a sample of 5 ml to examine it in the uv vis and I used 1 ml of the 5 ml for the Folin Ciocalteu method. How do i get to mg GA/g dry tea ?
my absorbance for example was 0,14 and 0,124
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What is the amount of the reagent used?
Is the standard compound amount one ml as the solutions to be measured?
Please give more information so that I can measure the concentrations.
best regards
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I have a very thick date extract which cannot dissolve in a small amount of DMSO due to the presence of sugar
what techniques can be used to remove sugars or to get pure polyphenols or flavonoids?
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Dear research
How can you be sure that this viscosity is due to sugars?
Most of the materials that make the extract thick and difficult to dissolve are phenolic substances with high molecular weights, as well as high turbines with molecular weights similar to rubber, where they are dried.
These impurities can be eliminated by purifying phenolic compounds and separating them using the acidification of the extract solution.
One moleartic acid can be added to the extract up to an pH=2
Then the phenols are withdrawn using chloroform several times using separating funnel
Best regards
Dr. Naser Jawad
University of Kufa Hanan Sa
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Questions to consider: (1) Do we find evidence of certain bacteria overpopulating/underpopulating in patients diagnosed with colorectal cancer? Are they inhibiting or contributing to cancer cell proliferation and growth? How would we measure that?
(2) "It is now recognized that the gut microbiota and chronic liver diseases are closely linked." Would we find evidence of liver dysfunction/malfunction in those diagnosed with colorectal cancer? ( Quote from paragraph entitled "Liver disease and the gut microbiota" in .)
(3) If the 3 ‘P's’ for gut health are probiotics, prebiotics and polyphenols, then what effect, if any, would a SIGNFICANT increase in one of these have on a colorectal cancer patient's length of survival?
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Hi Samy Azer thank you for sharing and reaching out! I currently am not doing a research project on this. When you suggest I submit a method study, are you suggesting I summarize current methods and their effectiveness (e.g., a lit review)?
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I am trying to extract lectin (protein) of brown macroalgae that very rich in polyphenol content. Polyphenols can interact with proteins leading to soluble or insoluble complexes formation that can affect the bioavailability properties of the protein. Is anyone can suggest a method to separate the polyphenol from the protein extract?
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Please refer to the following RG answer which deals with this topic.
Dr. Diosady at the U of Toronto have worked extensively with this kind of separation using membrane processes, in chish it can be removed up to 80-90% of phenols.
In their method they use alkaline extraction followed by isoelectric precipitation, coupled to ultrafiltration and diafiltration processes to concentrate and purified the protein extracts. In a study by Xu et al. 2000 it was reported that about 50% of the total phenols were unbound at the extraction pH (alkaline). A treatment with 0.05 M NaCl could break phenolic-protein complexes ionically bonded accounting for about 30% of the the phenols in the extract. Finally a 0.1% SDS treatment can release nearly 25% of the condensed tannins which are bounded to proteins by hydrophobic interactions.
Hope this helps. Regards
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During the course of the cultivation of a Pseudomonas sp. strain on a toluene derivative we observe a strong darkening of the media, supposedly because of polyphenolic (polycatecholic) compounds formed. The same obervation can me made sometimes while bacteria grow on aromatic compounds . 
We like to isolate RNA from the culture but obviously the cells and later the RNA strongly sticks to the polyphenolic/dark precipitation in the media. Does anyone has experience with that and a solution for that? (The RNA extraction from cells grown on succinate work perfectly.)
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Since I am working with carnivorous plants, which produce a high amount of polyphenols under stress, we had some problems with DNA extractions and PCR.
After some research, I found out some solutions, which may also work for you.
Since you are observing a strong darkening of the media, we can assume that you have oxidized compounds, probably caused by oxidases. If that is the case, you will need to change your extraction method from the beginning.
Some oxidases can rapidly cross-link compounds to RNA or in my case DNA, during the disruption of the cells, so you need to prevent this to happen.
The best way is to use liquid nitrogen before you disrupt the cells, this will inhibit the work of possible oxidases.
Then you could try to adapt protocols for isolation of plant RNA and DNA that are developed to remove polyphenols and other contaminants or prevent oxidations.
Examples: PVP (polyvinylpyrrolidone) (remove polyphenols), beta-mercaptoethanol (reduce oxidation).
I am attaching one protocol as an example, but you can also find several protocols for RNA extraction of plants.
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I havee trying to extract and purofy proteins starting from fresh plant material but always getting a lot of oxidations and polyphenols sticked to my proteins. So i would like please to ask if you have any suggestion or protocol to overcome this problem, where it is blodking my experimwnts progress. Thanks for your comprehension. Regards.
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