Science topic
Plasma - Science topic
The residual portion of BLOOD that is left after removal of BLOOD CELLS by CENTRIFUGATION without prior BLOOD COAGULATION.
Questions related to Plasma
I'm looking to measure estradiol in mouse plasma. There are several commercial ELISA kits available. My main question is if to use extraction prior to quantification or not.
Thanks
What are the side effects
Of plasma applications in medicine ?
Especially in surgical applications
For example
Resection of Liver Tissue
Freeing Bowel from Abdominal Wall

I have fibrin from human plasma insoluble powder (Cat No. F5386-1G). I have to crosslink it with X compound but before crosslinking I have to make it soluble.

is it Qiagen miRNeasy Serum/Plasma Advanced Kit or Norgen Plasma/Serum RNA Purification Kit ?
Plasma drug concentration and brain distribution study
This question is related to possibility of experimental studies of electric plasmons.
More information about theoretical part of such studies
can be found in the preprint "Materials with negative permittivity or negative permeability – review, electrodynamic modelling and applications"
I must build a public system air installation project ( innocuous sterilization for humans and environments) that protects the air conditioning atmosphere layer.
so, I need to connect with 3 parts of the research. that first, classic scientific result of Plasma in Physics that "plasma treatment reasonable scientific research part". Second, the current product state of sterilization air system by plasma in the " industry part". Third, the system module that plasma sterilization treatment module process (easy and simply) "engineering system part".
Would it be possible to use the magnetic confinement as used in fusion research to contain the tin plasma that produces the EUV light for semiconductor lithography?
Hello researchers,
We have plasma samples stored at -20 degrees C since one and a half year.
Are they still okay for Elisa, qPCR, or any analysis?
We need some expertise opinion, and we would really appreciate your help.
I used the brain, plasma and serum sample of rats. Preparation of the samples were done on ice bath and centrifugation done at 4 C, then the supernatants were kept in -20 C. the test was done with in 7 days. The brain samples gave satisfactory results on lipid peroxidation test (using 20% TCA and 0.67 TBA). However, plasma and serum samples did not produce any color at all, what cause the absence of colour in these samples?
ps: I also have tried different preparation for the plasma, using citrate and EDTA.
Can one recomend any training (live or online) or webinar or something like that about plasma analysis during PVD processes, especially in terms of mass spectrometry ?
I would be very grateful for an advice.
I am trying to determine the electron number density in my plasma by using the Saha Equation for degree of ionization. However I am slightly confused with how to determine the particle density (n = ni + ni+1) to solve for ne. Is there a standard value or...?
I presume as a first approximation I could determine the density of the gas with the ideal gas law and then use this number to approximate the density at my determined temperature. If I do so are there any obvious complications I may be overlooking?
Thanks in advance!!!!
Dear colleagues, I would like to specifically purify an anti-HLA-A2 antibody from plasma samples of a kidney transplant patient. I believe that antigen-specific affinity purification might be the best option, but I'm not entirely sure. Does anyone have experience with this process? Thank you very much!
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
I am running a logistic regression analysis exploring the association between Alzheimer's Disease (AD) status (outcome) and plasma biomarkers of AD (predictors), namely, Ab40, Ab42, the ratio, as well as p-tau217 and p-tau181.
However, I have all the plasma AB measured in pg/mL, p-tau217 in u/mL, and p-tau181 in ng/L.
How can I convert these units to make them comparable?
I know there is a 1-to-1 correspondence between pg/mL and ng/L, but do not necessarily know what to do with u/mL.
Many thanks in advance!
Hi all,
I was wondering if people here have any recommendations on ELISA kits they've used and validated to quantify cfos protein levels in a biological sample.
Ideally using brain, but if it worked in your hands for brain, cells or plasma, please let me know what kit brand you used. I used one from AFG and was not very happy with the results.
Thanks
Mario
Hello, I am an Etch Engineer.
I am performing etching with high T_ox/Nit selectivity using remote plasma.
Etching plasma is formed using only NH3/NF3/H2.
1. I wonder if it would be advantageous to add Ar or N2 as inert gas to increase the stability of plasma. (There is an ion filter, but I am concerned about damage caused by Ar)
1.1 Additionally, it is said that inert gases help maintain plasma density, but only the radicals of the reactive gases react, not the electrons or ions needed to form plasma. Is there a difference?
2. I suspect Nit etching due to F radicals when using that gas. I am curious about a way to reduce the concentration of F radicals. (Additional process gas, ratio, etc.)
3. Is there a pre-treatment process that can reduce Nit etching?)
Thank you very much.
Magnetohydrodynamic generators are normally used as eihter an attachment to recycle wasted heat and flow of other generators, or used to absorb the energy from rocket thrusters.
However, I'm trying to understand why not use the MHD's as the primary source for power generation while using configurations such as a Tokamak or a Stellarator to confine its combustion and plasma?
Either using the combustion of fossil fuels or the combustion of liquid oxygen/hydrogen rockets, it could be interesting to use it as a compact generator that directly converts electricity from the reaction.
Regardless of what I believe it could be interesting, the specialists on the subject do not use MHD generators in such a way. And I assume there is a good reason for that (such as its low efficiency), which I'm trying to understand.
The CCP type has a structure where plasma is formed at the top and only radicals come down through an ion filter.
I'm curious that even if ions come down, there is no bias applied to the susceptor, because then there is no force to accelerate the ions.
Even if the ion filter doesn't work, wouldn't it cause damage?
Thank you
How these waves form in such a superthermal plasma environment
I would like to implement a endogenous RNA-based internal amplification control for a RT-qPCR assay used to detect viral pathogens in human plasma samples. Are there any detectable endogenous RNA in human plasma? Any piece of advice ? Thank you!
I was wondering if the plasma that is in the top layer after centrifugation can be used to assess proteins, metabolites, hormones, or other molecules, or if the ingredients of the Ficoll alter the measurements. I have not seen any paper regarding this topic or people describing that used the plasma from this technique.
I am particularly interested in zonulin and cytokines.
How much energy is needed to form plasma, and is a vacuum necessary for plasma formation?
In cancer biomarker identification most of the studies uses serum, EVs, or plasma as as source of miRNA isolation and identification. Why whole blood is not used as a source? Whole blood miRNA profiles have more potential to show systemic changes as compare to plasma which has lower EV or circulating miRNA concentration. What is the probable reason for this discrimination for whole blood? how will it affect any clinical translational product scientifically?
I’m looking into bonding a PDMS microfluidic circuit to a PI sheet using a plasma activation method. The PDMS will be on the order of ~700 um thickness, while the PI will be 12.5-50 um. I have read a paper on nitrogen plasma bonding of the two but in their case, they spin-coated the PI onto PDMS. Does anyone know if it’s possible to use such a method on two separate solids? Or preferably even an oxygen plasma bond method?
Hi,
I'm doing a test to assess the hemolysis of some plasma samples and I have seen that one of the best methods is to see the absorbance at 414nm using Nanodrop (reference: ).
I tried using the Nanodrop 2000 and the Nanodrop One, but the results are really different, for example, in the first sample in Nanodrop 2000 I obtain a value of 0.075AU and in the Nanodrop One a value of 1.75AU, and this is the same for all of the samples.
Someone knows the difference between how the Absorbance Units are calculated in both cases?
Thanks in advance,
Lluc
How does the choice between albumin and donor plasma as replacement fluids impact the effectiveness of TPE?
I have simulated an argon plasma in a coaxial structure by COMSOL. To do this, I have used two interfaces, Plasma and Laminar flow, but in the period I have studied (microsecond), the shape of the flame does not appear at the outlet of the tube, and plasma is formed inside the structure.
When I simulated the movement of the fluid in the tube, the fluid formed its own jet-like profile in 3 milliseconds however the plasma formation occurred at 0.4 microseconds.
1. Can fluid at the plasma interface be defined as flowing from the beginning?
2. What other steps are needed to link these two interfaces?
please guide me. thanks...
What is the difference between the ways of the generation of thermal plasma and non-thermal plasma?In other words,if we generate plasma by a certain method or reactor,how can we determine what kind of plasma it is (NTP or TP)?
I stored blood sample at -88 five months ago, I would like to ask if there is possibility to thaw them again for isolation of exosomes?
Dear colleagues,
I require urgent assistance regarding the authenticity of the "Plasma Science and Technology" journal. Upon investigation, I found two websites bearing the same name and ISSN number "ISSN: 1009-0630":
This discovery raises concerns about the legitimacy of one of these platforms. I seek your expertise in discerning the genuine journal from any potential fraudulent counterpart.
Your prompt assistance in clarifying this matter is greatly appreciated.
Warm regards,
The inquiry relates to a study conducted by Alkouri, Rana, et al. in which they investigated the stability of various biochemical analytes in whole blood and plasma samples stored for different durations before and after centrifugation. Twenty-four analytes were measured in plasma samples, showing variations in concentrations over time.
This inquiry pertains to the study of Neuwinger, Nick et al. entitled "Underfilling of vacuum blood collection tubes results in elevated lactate dehydrogenase activity in serum and heparin plasma samples." I would like to know the effects of volume variation in blood samples on the accuracy of total cholesterol measurements in serum and heparin plasma samples.
In the laboratory, blood samples are often used to test for LDH. However, I want to know which sample is better as they are both blood samples.
The transition from gel separatory serum tubes to lithium heparin gel tubes in the clinical laboratory.
The entire point of Oguzhan Zengi's study was to improve turnaround time and sample quality by exploring the less traditional option of plasma for the above reasons. The results did not suggest an outright replacement of serum, but showed many positives and reasoning for such.
In a way, one might say plasma has been neglected. This begs the question: what other potential sample variations could be used to improve TAT and sample quality?
This question is derived from an article entitled "Underfilling of vacuum blood collection tubes leads to increased lactate dehydrogenase activity in serum and heparin plasma samples" by Neuwinger et. al. in 2019. I want to know how certain anticoagulants affect the lactate dehydrogenase activity in plasma or serum samples.
The research "The transition from gel separatory serum tubes to lithium heparin gel tubes in the clinical laboratory" is a study that aims to assess the viability of replacing serum samples with plasma samples in various clinical chemistry and immunoassay tests and to examine the implications of turnaround time (TAT) and sample quality during the transition process and a result of the study shows that there is a decreased TAT.
Oguzhan Zengi's study on the switch from gel-separator serum tubes to gel-separator lithium heparinized plasma (LIH) tubes in clinical chemistry was critically analyzed, leading to the formulation of this research question. According to the journal of Oguzhan Zenki, "The Transition from Gel Separatory Tubes to Lithium Heparin Gel Tubes in the Clinical Laboratory," there was a random selection of sample sources at the emergency department. Hence, this gave birth to the question concerning the result's accuracy.
According to the study "The transition from gel separatory serum tubes to lithium heparin gel tubes in the clinical laboratory", most clinical chemistry and immunoassay tests can be performed using lithium heparinized plasma (LIH) tubes instead of serum tubes, except for the lactate dehydrogenase (LDH) test. Lithium heparinized plasma (LIH) tubes have been shown to enhance healthcare quality, improve sample quality, reduce the incidence of aspiration errors, and lessen laboratory staff workloads in clinical settings. However, the reason why the LDH test cannot be performed in most immunoassay and clinical chemistry procedures that use lithium heparinized plasma (LIH) tubes is still unclear.
This question aims to understand the rationale behind the study, which likely sought to evaluate the comparability of gel separator serum and LIH plasma tubes in clinical chemistry and immunoassay tests. Additionally, it seeks insights into the study's findings concerning sample quality and turnaround time during the transition from serum to plasma samples, shedding light on the efficiency and reliability of different tube types in laboratory procedures.
We are trying to sputter a metallic target. We can clearly see the plasma however after depositing for more than 30 minutes there is no deposition on the substrate. What can be the reason for this? need expert advice.
Thanks!
I want to understand what the Warburg effect is and how it affects LDH activity in cancer.
I would like to ask about the thrust in PPT (Pulsed Plasma Thrusters) which uses solid propellants such as Teflon.
We know that the thrust force is given as F= T= d(m ve)/dt = m dve/dt + ve dm/dt. We know that both the mass and also the exhaust velocity ‘ve’ are not constant, the velocity usually fairly similar to the trend of the discharge current signal.
The question is:
Why the most (or ALL) of researches consider that the exhaust velocity 've' as constant value and neglect the second term of the equation? Is because the experimental measurements do not allow detecting the variation or something else?
Secondly,
If so, How about the theoretical calculations? Should we consider the variation of ‘ve’ or just take the constant value? and which one? is it the peak or the average velocity on the exit muzzle? average of peak velocity along the discharge tube?
Also, How about the specific impulse ‘Isp’ in both cases? Is it just as Isp = T/(g*m_dot) or ve/g and which ve?
I am planning to get some blood from patients to perform RNA seq (circular RNA which requires deep sequencing). I am wondering if anyone can share the protocol with details. I am also wondering about the amount of blood (whole / plasma / serum) to isolate RNA for deep sequencing. Thank you so much for your help!
How many ug/particles of EVs is needed for doing proteomics?
We are doing many steps for purify EVs of human plasma and at the end of the process we are getting just 1/2ug/ml concentration of EVs, which is about 2.17e+10 / 8.18e+09 particles/ml. What is the volume or minimal ug for doing proteomics?
Thank you!
Linoy.
What factors or physical mechanism will affect the laser produced plasma (LPP) expansion process. How to reduce the instability of multiple measurements?
Hi,
I have to extract genomic DNA from plasma samples and will use the Qiamp DNA mini and blood kit (QIAGEN). At the moment the plasma samples are stored at -80° and they were obtained from whole blood using a single centrifuge 1800 rpm x 10min. I would like to know whether it is appropriate to centrifuge the sample (and thus concentrate the remaining cells into a pellet) before proceeding with the addition of proteinase K, and if so, how should the centrifugation be performed?
Thanks!
What is the most efficient time to oxygen plasma of PDMS to increase the hydrophilicity?
The subject of the study centers on the switch from gel separatory tubes to lithium heparin gel tubes in the clinical laboratory but the study additionally addressed the use of the Barricor mechanical separator heparinized plasma tube that was acknowledged by Hetu et al.
I am trying to detect CCK in plasma with the antibody. if anyone knows how to detect and how much to load.
why plasma plume turn to be longer at a lower pressure? Is that attribute to pressure?

can FBA be substitute of plasma
I would like to know why an FFHR's plasma has no need for high power amplification performance.
The research titled "The transition from gel separatory serum tubes to lithium heparin gel tubes in the clinical laboratory" is focused on estimating the potential of replacing serum samples with plasma samples in various Clinical Chemistry and Immunoassay tests. According to Zengi (2023), plasma is gradually gaining popularity over serum due to the advantages it provides. It has several advantages, including greater volume, no need to clot prior to centrifugation, and improved sample quality. However, it is not clear as to why plasma has a larger volume compared to serum regardless of having the same volume of blood drawn.
Gel separator serum tubes and gel separator lithium heparinized plasma tubes are commonly used in clinical laboratory practices for sample collection and processing. The choice between these tube types can have implications for various aspects of laboratory operations. Understanding the factors influencing the choice between these tube types is essential for optimizing laboratory workflows and ensuring reliable diagnostic testing.
The development of this research question arises from a critical review of the study conducted by Oguzhan Zengi on the transition from gel separator serum tubes to gel separator lithium heparinized plasma (LIH) tubes in clinical chemistry. Hence, it emerges from a desire to delve deeper into the practical implications of adopting LIH tubes in clinical chemistry practices.
For my tantalum pentoxide thin film integrated capacitors, I need to etch the tantalum and tantalum pentoxide layers with pure SF6 plasma. While etching these layers copper will also be exposed to the plasma. Does anyone know if the copper will be etched too (and if so at which etch rates) and if it reacts in a form so that the copper will no longer be conducting?
If electron temperature and electron excitation temperatures in plasmas are different, then, is there any case they will be equal or always remain unequal?
difference between excitation temperature and electron temperature in plasma
what is excitation temperature of electron in plasma
I am currently engaged in the analysis of a collection of previously obtained samples, which primarily comprise frozen whole blood. My specific requirement pertains to isolating the plasma component from the aforementioned samples.
However, upon thawing, I have observed a phenomenon wherein the red blood cells (RBC) appear to have undergone lysis. Consequently, I am encountering challenges in effectively separating the plasma from the cellular constituents of the blood. Despite employing the Ficoll-paque gradient method, my endeavors have been met with limited success.
I would greatly appreciate any suggestions or guidance you may have in overcoming this issue.
Thanks!
Our process involves generating plasma with Ar and Hydrogen gases, followed by the deposition of Hydrogenated Carbon.
Oral 2mg diazepam, half-life 20 h, bioavailability 90%, what is the concentration in saliva after 14 h?
knowing that the saliva drug concentration is 0.1 of the plasma concentration.
When two intense laser beams interact with a plasma, is second harmonic generation produced?
I am determining butyric acid in plasma.
For this, I am developing a method. I will be giving a brief on how I prepare my highest concentrated calibration standard.
I prepare my internal standard (d7- butyric acid) in 100% acetonitrile having a concentration of 100 uM. From which, I transfer 10 uL to a cold glass vial along with 10 uL of 10 mM of Butyric acid as my highest concentrated calibration standard. I use 10 uL of 20% PFFBr in 100% acetonitrile to derivatise my standard and heat it for 1 hour at 60*C.
- The problem I am facing is low signals of internal standard to an extent that it cannot be even detected within the given retention time.
- Also, I am receiving signals for butyric acid in my blank and no peak for internal standards.
Could you please help me?
Hi, everyone!
I am a graduate student of pharmacology from China. I am trying to measure the plasma NETs level with anti-MPO antibody and Sytox Green, which are available in our lab. Here's how I did it.
Firstly, a high-binding 96-well plate were coated overnight at 4 ℃ with anti-MPO antibody(1 μg/mL, Thermo). The plate was washed 1 time with wash buffer, then blocked with 4% BSA in PBS supplemented with 0.05% Tween-20 for 1.5 hours at room temperature. The plate was washed 3 times again, then incubate with plasm (100 μL) for 2 hours at 37 ℃, 300 rpm. The plate was washed 5 times before incubating for 15 minutes with Sytox Green in dark (100 μL, 1:1000, Thermo). The fluorescence intensity (excitation at 485 nm and emission at 535 nm) was quantified.
But there was no difference in fluorescence intensity between plasma and negative controls. I'm not sure what went wrong. I hope anybody who did it can give me some advice. Thank you so much for your generous help!
Best wished!
Yafei, Fang
when extraction of RNA save the blood with trizol or serum or plasma
I mixed EDTA mouse plasma with standard gel sample buffer and got very smeary image on Western with no distinct bands. Is there something in the plasma that needs to be removed before I run gels?
I red a paper on an important journal of cardiology. In this work they found that 8 miRNAs were dysregulated in plasma samples of a study cohor of 1710 participants (Controls + patientes affected by Heart failure). I evaluated these 8 miRNAs in plasma samples in a study-cohort of 129 subjects (Controls + Heart failure patients) and I found that only 2 miRNAs were dysregulated according to the work I red. Because I have to explain thi data in "Discussion" I don't know how to explain this difference. Why could be the main reason of these different results? Maybe the size samples or other? Thank you
Are there some references which show measurement results?
I am running some pulldown experiments using plasma samples(click biotin linker on protein, then blot against Strep-HRP). We started the experiment with a known protein (Lane 2-5) then plasma samples (Lane6-9), lane 5 and 9 both incubated with alkyne-biotin. Although the signal is very small and on WB I need to overexpose to see it, but the bands on lane 9 is giving a bizarre signal. Could anyone help me understand the reason for this and how to solve it?