Questions related to Plant Secondary Metabolism
I experimented the modified vanilin method and for the calibration curve i used cathechin but there was no result. All my samples were without color. Please help me.
It seems there's a number of brands and companies to choose from, and no one I know has performed this test. Abcam's kit seems to be the most widely used from the literature. Do you have any brands to recommend or any secret tips to share?
I am a MSc student of plant biology and I am looking for a researcher who either have an interest or that studies the differences in the secondary metabolism of plants under space conditions or stress in general that can be applied to space research (radiation, microgravity, hipoxia, drought, mineral nutrition, etc). I am also interested in researchers that work with molecular biology of plants under the same conditions.
I am asking this because I am trying to find a lab for my PhD. I have a great interest in the subject!
I am currently working on a paper on secondary metabolites of brown algae I am having a hard time finding a good reference for the Rf factors of the different compounds which are common in plants and algae (e.g. alkaloids, terpene). I only used thin layer chromatography (TLC) for the separation of the different secondary metabolites. Are there any references that you can suggest that I use for this paper?
I worked on two related plants. I want to identify that both plants contain the same secondary metabolites by taking a single metabolites.
Suppose one wants to elucidate the pathway of a plant secondary metabolite, or of a class of plant secondary metabolites (e.g. alkaloids) in a certain organ (e.g. fruit). How should this question be addressed? Where should one start and proceed? Which techniques can be used in each step, and for what reason? There is no need for details on procedures, just the general idea.
It may be a too general question, with perhaps some obvious answers, but it would be really useful for me, who have never worked in this field.
Thank you in advance.
I need some opinions in terms of gene expression and secondary metabolites for plants grown under artificial light. I need to know if there are any big differences in terms of (anti-oxidant, flavanoids, pigments and other secondary metabolites) between plants grown under aritifical lights and plants grown under natural sunlight. Opinions with relevant citations would be much appreciate.
I'm new in the area of plant secondary metabolism. So i wanted to know if there is any way to genetically express some specific secondary metabolites (Alkaloids, Terpenes, etc) on diferent microorganisms (Bacteria, yeasts, microalgae).
I need a database or books for known compounds NMR data. I isolated some compounds and their NMR data something different for this case, I am thinking these compounds are the same type.
I tried to separate column fractions by LH-20 but unable. I used a different percentage of chloroform and methanol or higher percentage of methanol to chloroform. All the case, I am unable to purify the compounds.
Hydrophobic compounds are solubilised in water by using hydrotropes. After extraction, recovery of the target compound is done by dilution. Does anyone have example of using hydrotropic extraction at industrial scale?
Plant material is normally extracted after shade drying and grinding. Pulverized material is then extracted through different techniques like cold maceration or through soxhlet apparatus. If someone wants to get crude extract of a pulpy plant tissue (e.g., Aloe vera leaf or Broussonetia papyrifera fruit), what will be processing steps as shade drying for such tissues is rather impossible?
Ask about can be extract this component individually or combined from food or plant origin .
If i can i need the extraction method and determination
My present research is focusing on the evolution of plant secondary metabolites within a particular group of plants of medicinal importance. I have gone through a review of Wink which I am enclosing. As we all know, this phenomenon occurs because of convergent evolution. But to establish it in a scientific manner how should I proceed. Any constructive opinion is most welcome.
I’m planning to search new secondary metabolites from rice which will be phytotoxic. I read about various methods for identification and quantification of rice allelochemicals and would like some advice about recommended techniques. I'm really looking to find out what you'd consider to be the most efficient method as cost and time are limiting factors.
I made some ethanolic extracts, but I don't know which are the factors (temperature, pH) involved in vanillin degradation that may alter those samples before HPLC analysis.
so anybody have any idia how to convert this non solid material to solid constituents ,i mean if any gentleman done this work for any plants can explain it how to separate this extracted to solid parts ,thanks for your answer
Which is the best method to separate and characterize compounds from Crude plant extracts (Polar and non polar solvents )?
Please suggest different techniques.
Hi, I am doing derivatization of luteolin (standard) for GCMS analysis. My derivatization reagent is BSTFA and TMCS (99:1 ratio). I tried diferent temperature (60, 70,80,110) and time (15min, 30min,45min,60min), but still cannot find my luteotin. Can anyone give me any suggetstions?
I am doing derivatization of different flavonoids (Quercetin, morin, rutin, luteolin, apigenin-7- glucoside, luteolin-7-glucosie, catechin, taxifolin) using BSTFA and TMCS reagent (99:1 ratio) for GC/MS analysis. However I got always a peak which does not correspond to my flavonoid analyte, where the NIST library identifies it as fatty acid.
I do the derivatization as follows: the standard is dissolved in minimum amount of methanol, then ethyl acetate is added, followed by the derivatizing agent . the resulting solution is then heated at 100 C for 90 minutes, and then one micro liter is injected into the GC/MS.
Glucosinolates; plant secondary metabolites are known to be very effective against cancer, but some reports suggest that they are also toxic if used as primary food source. Does any researcher has any published material on toxicity limits of these compounds?
The non-treated material is comparatively soft and cannot be cut to make a thin section (about 30 μm thick). And I want to prevent several seconfary metabolites (water and alchol soluble) dissolving from it by various embedding treatments.
I would like to quantify some of phenolic and flavonoid compounds by HPLC. How can I extract these compound from plant and what is the solvent of HPLC with what program and percent of solvent?
I am looking to extract hesperidin, a known flavanone glycoside, from J. sambac roots. However, I would like to know if there are any other techniques I can implore in order to further increase the yield and also increase the yield's purity. Any help would be greatly appreciated.
Does anyone have experience with detergents for a solubilization of membrane proteins that conserves a specific membrane-protein sterol interaction?
Is there specific elicitors to increase specific compound or a general elicitor is enough to increase all the secondary metabolites? How can one choose elicitors? Is it plant specific, desired compound specific or something else?