Questions related to Plant Biochemistry
I have been working on Cupressus arizonica. one of most noted things is a layer of glaucous covers almost entire tree body. Still, I did not find any mentions relating to the purpose or causes of this layer in those books or papers.
In our lab we usually dry arabidopsis seeds(Col-0) in 37 degree incubator after haversting. But I found several protocols in which drying seeds in room-temperature. So im worried about whether 37 degree is too high for arabidopsis seeds？
Is it that chlorophyll content and protein level are significantly affected under drought?
Do drought-tolerant genotypes produce specific secondary metabolites? If yes what are the secondary metabolites?
What should one look for (in terms of biochemical parameters) when studying drought tolerance or sensitivity in legume or cereal genotypes.
Please see the link below:
I am trying to design an agricultural and horticultural calendar for Karbi Anglong and Dima Hasao districts of Assam, India. What are the steps that I should begin with?
I'm going to use half strength MS media for root initiation for indica rice cultivar. What will be it's composition when I'm using MS salt, sucrose and agar without growth regulators
Thanks in Advance
Can the hypersensitive response occur as part of PAMP-Triggered Immunity (PTI) as well as Effector-Triggered Immunity (ETI)?
Are resistance (R) genes expressed in response to pathogen attack - or only defense genes - as part of the plant immune response? R proteins act as receptors to recognize pathogen effectors and an interaction between R protein and effector stimulates Effector Triggered Immunity (ETI) which itself involves the expression of defense related genes. However, are R genes also expressed as part of ETI?
I'd like to know about the antibacterial compounds of those species. Can you suggest any previous researches?
for oxalate, i used 1g sample, 75ml 0f 3M H2SO4 to soak it. filtered and took 25ml aliquot and warm and titrate against 0,05M KMnO4. (1ml KMnO4=2.2mg).
for phytate, i used 2g sample, soak with 100ml 0f 2%HCl, filter and took 25ml aliquot and to it, 53.5ml dH20 was added. 10ml 0.3% ammonium thiocyanate soln was added and titrated against standard iron III chloride containing 0.00195n iron/ml.
how do i determine these values in mg/100g?
thanks. swift response is anticipated
Are plant pathogenesis-related protein genes, mlo genes, and SNARE protein defense genes classified as resistance (R) genes? Or do they fall into a different category?
Hi I am trying to express my protein R in tobacco leaves and do a CoIP, but I can't detect the protein R in CoIP input.
I took the same tissue and added 8M Urea plus LDS protein buffer, protein R shows as a distinct band at the correct size. But when I grind the tissue and put in CoIP lysis buffer, after centrifugation, I put the input in LDS, I couldn't detect my protein, I only see a smear.
Does anyone know what went wrong?
We are trying to isolate the mesophyll protoplasts from dicot leaves. So we are using an enzyme combination of cellulase onozuka R-10 and macerozyme R-10. The problem we are currently having is we can even hardly release the cells from the plant tissues. Using the microscope to observe every 1 hour for several hours, we didn't find any significant difference. My understanding is macerozyme R-10 is mainly aimed at releasing the cells from the plant tissues and cellulase onozuka R-10 is mainly aimed at digesting the cell walls. So based on the current problem, should we increase the amount of macerozyme R-10 to enhance the release of the cells? We are also considering to use pectolyase Y-23, so will this help? My last question is if the plant protoplasts are always round shaped or they can be elongated shaped? Any advice and experience about these questions will be greatly appreciated. Thank you very much in advance.
I want built a small biochemistry lab for research purpose ( Phytochemical Study).
It refers to the extraction, screening and identification of the medicinally active substances found in plants. Some of the bioactive substances that can be derived from plants are flavonoids, alkaloids, carotenoids, tannin, antioxidants and phenolic compounds.
Please give me some guideline and references for built a small laboratory.
Comment those journals which are going to receive its first impact factor in JUNE 2020 and currently indexed in ESCI, SCOPUS, PubMed, etc.
Journals must be related to plant physiology, plant biology, plant sciences, agriculture, and biotechnology, etc.
Note. Please don't comment any predatory journals. Thanks in advance.
i have got my mass spectrometry results on excel file but now i want to know how could i identify which proteins can be determined (identified) and which can't be identified? and on what criteria? qnd if there is any software that can help me in doing this?
thanks in advance.
Hi. I am making a research work in a course on my master degree in biotechnology (not a thesis). The assay is about finding new plants with antimicrobial activity. Plants without any kind of investigation in this area.
I prepared extracts of different plants (10 grams dry plant to 200 mL of solvent) in 99,5% ethanol and I just tested them with the Bauer method (disks).
I now have ethanol extracts of 7 different plants wich already proved to be efficient in some ways against Gram + and Gram - bacteria. But I need to quantify my phenolics.
I never used Folin-C. reagent, neither anything like it so my experience is really zero on this. I read some articles and even the SIngleton et al. paper about this and I can't seem to figure it out how to apply it to my study.
I can't change my solvent due to time and working limitations on the lab, so is it possible to make the FC quantification test for the TPC with ethanol as a solvent?
Can someone please tell me the steps I need, considering I have the FC reagent avaiable and gallic acid as a standard (and I don't know how to use it).
Thank you so much
I want to design specific primers to amplify a gene using qPCR. I already have many sequences geted by Blast method.
I used Primer3Plus to design primers, then to verify specificity of these primers I blasted each primers with genome of species on which I will work after. But when I blast these primers, they are hybrided in a multiple locations in the genome.
My questions are:
I can consider these primers as specific?
If not, is there a method to specify these primers?
Can you help me to resolve this problem..many thanks.
I'm currently looking for protocols for measuring reducing sugars content in seeds from Passiflora crops. In my review, I have found that the dinitrosalicylic acid assay (DNS) and Nelson and Somogyi methods are repeatedly recommended. And even though I've read some papers comparing these two methods, the comparisons these papers have dealt with are regarding the measurement of enzymatic activity rather than reducing sugars content.
I would appreciate if you can provide me with any references or insights regarding the pros/cons of each method for the measurement of this variable.
Thanks in advance,
Carlos A. Ordóñez-Parra
α -tocopherol is the major vitamin E present in green plant tissues, more specifically in the membranes of chloroplasts and thylakoids.
I performed many protocols to detect and quantify α -tocopherol in Arabidopsis thaliana, tomato, tobacco and grape vine extracts using HPLC (C18 reverse-phase column, methanol as eluent, UV detection 292 nm). The problem is that only one protocol has worked and only on Arabidopsis extract !!! (De vasconcelos et al. 2010. Evaluating the potential of chestnut (Castanea sativa Mill.) fruit pericarp and integument as a source of tocopherols, pigments and polyphenols).
More and more of world's landscape is getting degraded as a consequence of environmentally detrimental anthropocentric activities. During the 21st Century, when life support system is getting increasingly scarce, humanity's preoccupation must be to restore earth's degraded landscapes. Can mycorrhizae assist in this?
what is the best method to extract and evalute the antimicrobien activity for calotropis procera, like antibacterien & antioxidant activity
we are try to comparing total cellular content like chlrophyll ,carotenoid ,anthocyanin, proline ,sugar ,antioxidant enzymes of medicinal plant at different altitude level of Himalayas , know about the plant response to abiotic stresses . Will it show any significant result. what else we can do to make our project more informative ?
I am looking for a protocol by which I can detect the Ion-leakage in the anthers of my Arabidopsis Mutants.
I want to know the detailed protocol mentioning the tissue amount (anther numbers) and steps to be followed.
Which one is more suitable to measure heavy metal uptake by aquatic plants.
Can anybody provide me these 2 equations with references?
The culture is 4 months old and I have used activated charcoal (1%) and citrate (1%) to avoid browning of the PKB's and Seedlings.
It seems there's a number of brands and companies to choose from, and no one I know has performed this test. Abcam's kit seems to be the most widely used from the literature. Do you have any brands to recommend or any secret tips to share?
I want to measure oxalic acid from spinach plant so at the first I need to extract that, but I can't find any protocol about it. Can you help me?
Does anyone know dubois (1951) formula for calculation of sugar in plant extract? I have already calculated soluble sugar content in the sample using the standard graph developed using pure glucose.
The net photosynthetic rates of plantlets grown in-vitro decreased with
increasing sugar concentration in the medium.
I know that cells under tissue culture conditions are different from those in vivo in many ways, but what exactly could trigger antioxidant enzymes such as POX, SOD and CAT in tissue culture media?
Is There a simple method to prepare the extract of leaf, stem and root in order to estimate Na, K, Ca, Mg, Mn, Zn, Fe.
Thank you for your contributions
Recently I have gone through a discussion where they showed that under nitrogen deficient conditions the total carbohydrate content of microalgae increases significantly. as per my knowledge it is not possible if we provide the nitrogen deficient conditions simultaneously we have to provide the other carbon source like glucose, sucrose etc then only we can expect the increase in the total carbohydrate content in the algal biomass.
I have visited many historical botanical gardens, but I think that the situation is not one of the best, what are the most serious concerns that face the existence of these botanical gardens? how could the experience of a very expert curator help new botanical gardens to survive?
In rice, under low light condition, the height of the plant increases in comparison to the plants in the normal light environment. Under low light stress, the rate of photosynthesis(P) decreases. As the height of the plant is more so the food the plant produces is utilized for sustenance, instead of grain filling. So if the flag leaf is isolated at the flowering stage for the estimation of total soluble sugar(TSS) then (P) will be always positively (significantly)correlated with TSS. But as the TSS not promptly converted to starch under low light condition, there will be a non-significant correlation between the (P) and starch in the grain. However, under the normal light, the (P) will be positively correlated with TSS in the leaves at flowering stage and starch in the grain.
IS THIS ANALYSIS CORRECT?
Hello every on,
Pease I need the protocol for the Dark treatments of seedlings of Arabidopsis. If anyine know how can I do that please help me .
We try to make determination total polyphenol and chlorophyll from younger leaf until mature leaves (from first leaf until 4th leaf). is there any correlation between total polyphenol and chlorophyll in leaf, actually betwen young leaf or mature leaf?
according to various research paper many researcher prefer soxhlet method for extraction of palnt material. i need some more information about this method.
Ammonium nitrate is banned at present. So it is urgent to find the alternatives to carry on tissue culture.
Many articles suggest that these compounds are abiotic elicitors but they are synthesized in plants! Pls suggest
Hello! I would love to know about the production of secondary compounds, especially about phenolic compounds which are produced in the process of culturing plant tissue. It is supposed to be negative effects for the plant tissues, isnt it? Can anyone help me to explain more about this, and can we eliminate this phenominon? And, it would be better if you can give me the links or references as well. Thanks a lot!
Please colleagues, I need to know how to convert the measure of enzyme activities in plants (Ascorbate peroxidase and Glutathione reductase) from the SI unit of U/L to umol/L. I found that most literature did not report in the SI unit, so I want to convert to make the results comparable to other studies. Kindly assist.
I have tried to separate using Kraay et al 1992 methods using ODS UG 5 HPLC column, however the chlorophyll c1 and c2 cannot separate properly.
I have grown Arabidopsis in liquid MS for 10 days then I transferred them into 5 micromolar coelenterazine for overnight and I add Jasmonic acid isoleucine for 10 mins. now I want to rescue this plant... so tried to transfer them back to same liquid media, MS agar plates also on the soil. but every time these plants don't grow.. I am fed up of trying to rescue them.. can anyone suggest me something to rescue them??
we are working on improving salinity tolerance in plants using various strategies. one of the biochemical indicators of salinity tolerance in plants is the synthesis of the amino acid proline.
In response to senescence, silver is quite popular in blocking ethylene receptors (by displacing copper). I'm just wondering if it's effect extends beyond ethylene receptors to cytokinin especially in the spirit of cross-talk between these two PGRs
I have been told that I should sample only between 11h and 15h. Is it a raisonnable recommendation? Do you have any litterature supporting it?
I am attempting western blots on Arabidopsis seedlings (7 to 10d old). We have working anti-bodies and western blot conditions, but we are unable to find any signal. I suspect this protein is not very abundant. In addition, this protein may be rapidly degraded. What protein extraction buffers and conditions would be best for this type of assay?
I typically grind liquid nitrogen frozen tissue and then incubate on ice for 30 minutes in an extraction buffer that contains salt, protease inhibitors, triton, EDTA, and DTT in tris buffer. Any help would be greatly appreciated.
This is a related question to an older question of mine: https://www.researchgate.net/post/How_to_measure_free_phosphate_Pi_content_in_leaf_tissue.
I am trying to measure free phosphate (Pi) concentration in tomato leaf using molypdenum blue method (ammonium molypdate + ascorbic acid). I followed several published papers to extract Pi from tomato leaf with HClO4 10% (Bozzo et al., 2006, Plant, Cell and Environment 29, 303-313) and TCA 10% (Wilcox et al., 2000, Crop Sci., 1601-1605). Strangely enough, both methods caused serious precipitations with the molypdenum blue reagent, which interfered with OD reading. Increasing ascorbic acid up to 8% in the reagent solution and reducing extraction:reagent ratio to 1:10 (v/v) did not help. You can view the demonstration in the attached files.
Recently I tested the extraction with acetic acid 1%. The result was surprisingly good. There was no precipitation with molypdenum reagent and the color was strong. However, I couldn't find any reference using acetic acid to extract Pi from plant, which seems strange. My question is whether acetic acid 1% is capable of fully extracting Pi from leaf cell, as well as killing cell enzymes which might change Pi concentration upon extraction, and whether it hydrolizes any organic phosphate compound and change the free phosphate content. Any suggestion about the problem with perchloric acid and TCA or a better extraction solvent for this assay is also welcomed.
working on flower bud formation mechanisms of blueberry, I need to know about previous works on effect of CPPU, and auxin inhibitors on flower bud production in blueberries.
Does canopy diffusive conductance refer to canopy stomatal conductance, canopy transpiration, or canopy evapotranspriation? Or is it something else entirely?
I need to prepare substrate with concentrations of arsenic from 0.0 to 10 ppm. Which could be the best substate and preparation for this? I work with seedlings of a tree species.