Photo - Science topic

Hi there,

I am currently doing research about time restricted feeding(TRF). And here is the anatomic photo got directly on the TRF mouse(TRF with chow food). There are distributing lots of macroscopic white spots. I am wondering what are they? By the way, this mouse was sacrificed on the morning without fasting. Lipogenesis? glycogen accumulation? Fibrosis?

Hope someone could answer this question! Thank you in advance.

This is a transmission electron microscope photo of a special structure in secretory cells. I have looked up a lot of literature, but I can't find out what this structure is. I would like to ask what is this cell structure?

We often find new zones, hosts, geography, and country and state records of fungi. How far we are spending our valuable time on it. If it is a new record, we need to mention the latest feature from the existing one in a short note, or we copy all the information, including photo plates and add the latest information.

Are we just replicating the same information showing unlimited times?

Are we confusing the reader to find what is new in the ocean of copied information?

I have two access to researchGate : one is correct (with my name Nathalie Delzenne and my photo and full CV) and another one with Nathalie M. Delzenne, which is not correct) both are linked to my email adress : can you eliminate the one with Nathalie M. Delzenne and to keep the one with Nathalie Delzenne ? thanks

هل تعتبر الصور والفيديوهات المنتجة في برنامج الذكاء الصناعي جات جي بي تي اعمال ذات قيمة فنية؟

how ican calculate the relative abundance in camera trap result without number of individuals by using number of photo

is solvent system that produce distinct bands better or the one that spread the compounds over the plate as a smear ? . on which of the photos attached right or left TLC profile is showing better separation? As i read that the distinct bands mean better separation (as shown on the left photo) but they may also many compounds are co-eluted together and in the TLC profile giving one band . i doubt also that on the right TLC profile , the bands are spread more and this may mean that the polarity by this solvent system allowed good separation of the compounds of different polarities, achieving different Rf values on the TLC plate so please give me your opnion in that regard. Thanks

I'm looking for software that allows me to create highly realistic composite faces from a large pool of existing faces. For example, creating a typical face of a city from photographs of existing faces in the city. Another option is to create a database of standardized photos of people in the city and then use the software to create the "typical" composite face. What options do you recommend? Is Artbreeder really a good option?

Thanks!

(e.g., fragments, fibers, pellets, films). Based on their appearance in the images, could you please share your insights on their classification? Your expertise and suggestions would be greatly valued!"

Please see a convoluted discussion that might change our perception of world literature. Could the heralded literature revolution be what we need to stop the wars and achieve global unity? Please explore the FaceBook comments and tell me your thoughts:

This is a centric species with coarse areolae and two thick processes on opposite sides of the valve. Is it a kind of Aulacoseira? or Actinocyclus? Doesn't seem to fit the definitions for either of those quite exactly. The valve is convex, about 50-60 µm in diameter. The two thick processes are difficult to see in this photo but extend several µm above the valve face. It is encountered occasionally in coastal / estuarine deposits along the northwest coast of the USA. I'd appreciate any suggestions or advice on ID. Thank you kindly.

a collection of complications related to implants and implantation in orthopedics with intraoperative photos. where to publish

I have a photos under microscope

Hello everyone,

I took microscopic photos of a fresh state of a leachate from a wild landfill, the shape in the photos is the most common.

leachate tank had a red slick on the surface, i suspect a red algal bloom. I need help identifying the strain in the photo? Thank you so much in advance!

I need your recommendations for this issue.

My ResarchGate profile names were correctly captured but the photo and research papers belong to another researcher. Can someone help me correct it?

For two metals with large differences in corrosion resistance(for example, Al and Ti weld joint), during the corrosion process of metallographic sample preparation, one phase will be corroded completely while the other phase will be corroded excessively or not corroded, and clear metallographic photographs of the two phases cannot be obtained.

These species are photoed on Singapore Gardens by the Bay.

Many a times, Plant Ecologists have to click photos of plant specimens. There are other plants in the vicinity which may create confusion while identifying this one. In such a case, it is better to place a board or a thick paper behind the specimen to be recorded. As the plants are mostly of green color, what color should be the background paper such that the specimen is clearly seen. Should it be of dark color like black, maroon, dark blue, etc. or should it be light colored like white, cream, pastel colors?

Hello everyone, I am a first-year PhD student and I aim to measure the calculated total cell fluorescence (CTFC) in my confocal images using the stain CellROX Green. As you can see, some parts of the cell have high signal intensity. I believe that extracting the fluorescence as it is will lead to inaccurate data so I wanted to ask what is the best way to pre-process the photos in ROI with high signal intensity/overexposure before quantitative extraction of the mean grey value? I considered eliminating any overexposed pixels using the Threshold feature in ImageJ and eliminate pixels with an intensity of 200 – 255. I can apply this approach to all the images in my data set and proceed to calculate the CTCF after. What do you think of this approach? Is there a better way to pre-process these photos?

Below are examples of my confocal images. Thank you in advance for any advice. Looking forward to what you have to say.

I will be really thankful if someone can check my model

I need a camera lucida to draw polychaeta's chaetae and other details for taxonomical purposes, but I don't know were I can find one. On the internet I found only some museum piece. I know that I could take photos, but sometimes drawings are more suitable for taxonomic works, specially when details are not in the same focal plane. Is there a chance of getting one suitable with my microscope, that has 45° inclined eyepieces?

Why Mg centre is bent in some photos and linear in anothers?

"in CH3CH2MgBr"

Which of these items acceptable ? Why?

Mg has just 2 electrons in valance shell, why this centre can be bent?

We have the BXPC-3 cell line in passage 2 but when we do a few passages the cells change their cell morphology from epithelial to fibroid type. I don't know if anyone has had a similar problem and how did they solve it? I attach a photo of its morphology.

regards,

Abraham

1.A tall elastic structure that rests on the ground, consisting of small cross-sectional columns and beams, will never topple over, because their trunks do not have the necessary force to rotate it.

What will happen is the bases of the columns will remain glued to the ground, while the cross-sections of the beams and columns around the nodes will first elastically and then inelastically bend and break.

See first photo

2.A tall rigid structure made entirely of reinforced concrete such as prefabricated houses has the potential to topple.

See second photo.

3.A high rigid structure made entirely of reinforced concrete such as prefabricated houses, if it is placed on the foundation ground with ground anchors, will neither break nor topple.

and costs half the money. Simple things to beat the earthquake and bring the cost down.

Watch video https://www.youtube.com/watch?v=Q6og4VWFcGA

Hello Dear Colleagues,

I want to distinguish volcanic agglomerate in my study area, Is this rock in photos volcanic agglomerate?

I want to know the different between life time solar cell , degradation of solar cell and photo stability of solar cell .

I work on HSC-2 and HSC-3 cell lines in a 12-well plate with cell density 1*10^4 and I found a gradual decrease in cell growth over 3 days in the control group given that the passage number is P4. I observed the cells in a 100 mm dish for 6 days to check the confluence level and it kept going down as well. I found some bizarre shapes I didn't know under a light microscope and a contrast phase one. For contamination exclusion, I cultured the cell media on a blood agar plate for 4 days, and no colonies grew. I have attached some photos to help clarify my question.

Today animation is used as a multimedia technology with great educational potential, which goes far beyond just creating figures, as it can promote a better understanding in comparison with a traditional verbal presentation format. In this sense, the growth of organizations has been successful in the last decades thanks to the fact that the population has required new alternatives to solve the growing demand for services; these new possibilities that have been included in the productive sector favor organizations, which every day are concerned about finding new and better IT options to adopt them as support tools for the operational, regulatory and managerial functions of private companies and the public sector. photo: Adobe Stock.

UIC Link. Journal of the UIC Graduate Division 183year 3, no. 6, Jul-Dec 2023Training motivates personnel to perform better and is one of the key functions of management and personnel development in organizations; therefore, it must operate in an integrated manner with the rest of the administrative functions. As a result, more and more companies are developing content, mobile applications, management platforms and remote services that bring educational and training services closer to more people.

I have 17 different steel samples, in which i identified a peak of Ti 441.492.

But , if you look closely at photo, while some some peak are at 441.492 , some are at the slight offset. eg 441.490 ,441.488 etc

I am currently performing , Univariate Analysis for detection of Ti Concentration.

What could be the reason for this ? Also , while taking measurements , prior wavelength calibration was done.

Can , i choose such peak for Univariate Analysis ?

Also , how can i identify lines , which are prone to self - absorbtion ?

I am new into chemometrics , and currently into the learning phase.

Thanks and Regards,

Rahul P

Hi, I am working on heat stress in dairy cows. In a recent experiment, we used a FLIR-T62101 thermal camera to take photos of the cows. One photo is attached.

How can I analyze these images to measure the temperature of the cows? Could you suggest any related software or publications?

Every time i am facing this type if problem. I used volt free and 22mA current for the separation of whole cell extract proteins.During the run,my current flow fluctuates.What is the reason behind this scenario?

I have a problem in galvanostatic tests during the charge, as you can see in the joined photos, and I don't know the source of the problem, Please if any of you encounter this type of problem could you help with the answer?

Many research institutions have included among the main types of threats and risks developing globally in 2023 the question of the increase in the scale of organized disinformation operating in online social media. The diagnosed increase in the scale of disinformation generated in online social media is related to the use of applications available on the Internet based on generative artificial intelligence technology. With the help of applications available on the Internet, it is possible without being a computer graphic designer and even without artistic skills to simply and easily create graphics, drawings, photos, images, videos, animations, etc., which can represent graphically professionally created “works” that can depict fictional events. Then, with the help of other applications equipped with generative artificial intelligence technology and advanced language models, i.e. with the help of intelligent chatbots, text can be created to describe specific “fictional events” depicted in the generated images. Accordingly, since the end of 2022, i.e. since the first such intelligent chatbot, i.e. the first versions of ChatGPT, were made available on the Internet, the number of memes, photos, comments, videos, posts, banners, etc. generated with the help of applications equipped with tools based on artificial intelligence technology has been growing rapidly, including the rapid increase in the scale of disinformation generated in this way. In order to limit the scale of the aforementioned disinformation developing in online media, on the one hand, technology companies running social media portals and other online information services are perfecting tools for identifying posts, entries, comments, banners, photos, videos, animations, etc. that contain specific, usually thematic types of disinformation. However, these solutions are not perfect, and the scales of disinformation operating in internecine social media are still high. On the other hand, specific institutions for combating disinformation are being established, NGOs and schools are conducting educational campaigns to make citizens aware of the high scale of disinformation developing on the Internet. In addition, proposed regulations such as the AIAct, which as a set of regulations on the proper use of tools equipped with artificial intelligence technology is expected to come into force in the next 2 years in the European Union may play an important role in reducing the scale of disinformation developing on the Internet.

I have described the key issues of opportunities and threats to the development of artificial intelligence technology in my article below:

In view of the above, I address the following question to the esteemed community of scientists and researchers:

What do you think about this topic?

What is your opinion on this issue?

Please answer,

I invite everyone to join the discussion,

Thank you very much,

Best regards,

Dariusz Prokopowicz

Hi, I sometimes get these circles forming in my cell IF. They do not always show up, but sometimes I can't seem to avoid them even when I follow the same protocol. Any ideas what may be causing this issue?

The red signal is supposed to be cox4. I have included a reference photo to show the expected staining pattern.

I'm a quite new to growing cells and have only worked with HAC15-cell line and H295R-cell line. The HAC15-cells look very different from H295R, and I'm starting to wonder if they are healthy or not. However, I'm having a hard time finding photos of HAC15-cells. I wonder if someone here is working with this cell line and happen to have taken pictures?

Hi,

I want to calculate the plastic equivalent strain for Mohr-Coulomb. I found a formula from Abaqus documentation. I have attached the photo of PEEQ calculation. But I want to figure out the explanation how it came or how to expand this short form in step by step? Can you please describe me how can I go for it?

Hi there, researchgaters...

I am trying to find descriptions of the genitalia of Hermetia illucens, the black soldier fly (Stratiomyidae), but no luck.

I identified a couple papers that seem to contain this information:

If anyone out there has PDF of these or any other paper containing photos or images of the genitalia of this species, I will be most grateful to get a copy!

Thanks in advance.

Hello to every one. I intend to draw a plot in origin pro, and its y-axis be something like the attached photo. in this regard, i want my y-axis divied into two section. the the section one values on figure be 0, 20,60, 100, and the section two values be 1000 and 10000. could anyone help me regarding this issue?

It's not a question, but if anyone has a photo of the eggs of the fish called Harpagifer bispinis, please share it with me.

I am doing a replication of an experiment regarding attractiveness and I am looking for photos I could use in it.

I hope this message finds you well.

I'm writing to seek your expertise regarding image editing tools that can add scale bars to photos. I'm working on a research project that involves analyzing microscopic images of specimens, and accurately representing the scale is crucial for our analysis.

I've tried searching for online tools that can perform this task, but I'm having difficulty finding reliable and user-friendly options. I'm hoping you might be able to recommend a specific website or software that can effectively edit photos and add scale bars.

i need an easiest technique for Fluorescent stain used with Fluorescent Microscope .

for photo phenton reaction, dye concentration 10ppm, catalyst 100mg/L.

Paper concern the Yucca Mountain Nuclear Waste Repository project and the Waste Isolation Pilot Project.

Hi there,

at the moment I am doing a study on migrant worker in Singapore. During my research I met a return migrant who documented his journey with his camera and he gave me all his more than 900 photos for digitalization. It’s a real treasure for me because it gives me insight into the daily life of migrant workers 20 years ago. I would be interested in getting hints for interesting literature with a particular methodological focus how to analyze photos. What are the different steps? Is there may be a helpful software that can support to organize, tag and analyze photos?

Thank you very much for any hint or recommendation!  

We bought this cell line from ATCC, and I attach a photo of a plate after passage 2. There are many small dots around the cells and it is making me concerned/suspicious of bacterial contamination. However, the description of the cell line suggests that "MDA-MB-468 (ATCC HTB-132) cells can be slow to attach and may produce large amounts of floating cells and debris."

Does anyone have any experience with this?

Thanks!

Ana

Hello,

I have been cultivating RAW264.7 cells from ATCC recently. I culture them in DMEM medium + 10% FBS at a density of 10,000 cells/cm² in a T75 flask with a volume of 10mL.

For cell passages, I use trypsin to detach the cells.

During the first two days of culture, they reached 60% confluence after 15 hours, and I performed a cell passage. Then, after the second passage, it took them 8 days to reach confluence. I noticed a change in morphology on the third day after the second passage, with an unusual morphology of cluster formation. (Attached photos)

Could someone help me understand why these cells behave like this and why they take so long to grow?

Thank you in advance,

Last weekend I took a photo of this cave-dwelling snail in a northern peruvian cave (1200 metres above sea level). Could you please help me to identify the Genus and Family? Thank you very much.

Hi,

I'm in the process of developing a hydrolysis probe qPCR assay to quantify gene copy numbers of Microcystis 16S rDNA. In troubleshooting my cycling parameters, I'm getting resulting plots such as the attached photo, where a plateau phase is not reached but rather a secondary jump in fluorescence is observed in the late stages. I ran NTC samples on this run (they're labeled "Blank" in the photo), and no fluorescence was detected at all, indicating this is not due to primer dimers.

Does anyone have knowledge of certain assay parameters that may lead to a plot such as this? Possibly related to annealing or extension times/temperatures? Could inhibitors in my template cause something like this?

I need your assistance

As shown in the photo, the curved mandrel must be immersed in liquid TPU through a dip molding process.

However, the outside of the bent part will always be thinly coated.

Can you tell me the reason for this and how to solve it?

TPU has a high viscosity of 30,000 cps.

Thanks you.

Dear all,

Hello, I am recently working on isolating neutrophils from murine bone marrow by using Histopaque 1099 and histopaque 1077, but the purity of the cells are always unsatisfactory. Here I have several questions concerning the techniques. Firstly, which layer is neutrophils, could anyone help to illustrate this with photos or figures. Secondly, I am wondering what other techniques could be used to help to improve the overall quality of the cells.

Thank you so much for answering this questions for me. Best wishes to all !

I am wondering what tools people use to design these circuit layout schematics in 3D (as shown below).

I know illustrator can do some of this but what else is used for 3D designs of complex photonic/electronic circuits?

Figure References:

1. https://cemse.kaust.edu.sa/ipl/system-level-applications-integrated-chip-lasers

2. Bogaerts, W., Pérez, D., Capmany, J. et al.Programmable photonic circuits. Nature 586, 207–216 (2020). https://doi.org/10.1038/s41586-020-2764-0

I am a phd student in energy conversion mechanics in Iran. About six months ago, I wrote an article together with my corresponding and it has been sent to a reputable journal for acceptance, but unfortunately they have not yet responded. It is possible to guide me to accept and print my article. I will send you the photo of the first page of the article. ??????????

I'm searching for Silpha punctulata Olivier, 1790 male specimens, or images representing S. punctulata's male genitalia.

Hi! I found these organisms on a woman's stockings, by rinsing the fragments with serum, and I suspect their presence is the result of friction with a wall covered with mold and/or algae. These are relevant in a forensic case. I think the first 2 represent a fungus, and the last 2 pictures are some unicellular algae. Does someone have a more specific idea of what kind of organisms are in these photos?

Agrophotovoltaics is based on photovoltaic installations that consist of light-transmitting modules placed on structures higher than those commonly used on land. Thanks to this, you can farm, grow flowers, vegetables, fruit and cereals under the panels.

Photo from the Internet: https://www.enelgreenpower.com/media/news/2022/12/agrivoltaics-benefits-world-agriculture

Can anyone please introduce me a free software for measuring light and electron microscope when taking a photo (for light and electron microscope photos, as well as when working microscope) diameter, perimeter, and area in the form of Circular, polygon, and filamentous or tubular forms in terms of width and length?

What about color changes?

Thank you in advance,

Jafar sabouri

After several washings of shale samples in search of microfossils, each time we obtained the same results, a species of foraminifera and radiolarians, we followed the standard method, with water, hydrogen peroxide , soap, vinegar... but in the end it's the same