Questions related to Parasitoids
I am involved in a project on biological control of the Comstock mealybug Pseudococcus comstocki in Switzerland. As part of this project, we are doing host specificity tests of a parasitoid and, besides P. comstocki, have tested so far the following non-target species: Pseudococcus longispinus, Planococcus citri and Phenacoccus aceris. We would like to test more species of the family Pseudococcidae and are looking for someone in Europe who could give us an identified starting colony for this purpose.
Thank you for your help!
I want to know distribution, Species richness and abundance of insect pest predators and parasitoids along agro-ecology and farm types (field and backyard) which research design/methods is appropriate?
I am a post doc at ULiège in Belgium, and in my research group we are looking to buy a microbalance to weight the dry weight of parasitoids of drosophila larvae. These insects are super tiny (dw ~ 250 µg).
The company we started to discuss with, proposed us a Sartorius microbalance (model MCE3.6P-2S00-M). In the past I (and other colleague from the team) only worked with Metler Toledo microbalances, but MT ones are far more expensive (the price is almost twice higher). So the point is that we never used Sartorius balances, and we therefore don’t know the quality of this equipment. Does anyone have feedbacks on Sartorius microbalance to weight such small individuals? Thank you! Best regards, Thomas
I have a dataset composed of aphid and parasitoid abundances captured in Moericke traps on a monthly scale for 10 years. As I do not have data on parasitism, but on the occurrence of aphids and parasitoids, I cannot use common trophic networks. In this way, I think I could explore some community-level relationships through correlation-based networks. However, I would like to know if there is any impediment to using this approach or if anyone has already used it.
Thyrinteina arnobia (Lepidoptera: Geometridae) is the major defoliator of eucalyptus plants in Brasil in the last 50 years.
Since them, no parasitoid had been recorded for eggs of this pest in the field. In te lab we demonstrated that some kind of substance on the surface of these eggs protect them.
In 2017, we a found a parasitoid in eggs of Thyrinteina arnobia (Lepidoptera: Geometridae) in the field.
I would like to know who can identify this parasitoid. The quality of the photo is very poor but I can take better ones if necessary.
José Cola Zanuncio
Botanical insecticide is the natural insecticide which extracted from plant materials.
Botanical insecticide contains several secondary metabolites.
On the other hand, the volatiles released by plants (when induced/attacked by herbivores) to attract predatory insects and parasitoids are included in the group of secondary metabolites.
Based on the explained above, so that, a question arises from me to my esteemed research friends.
The question is: "Can the botanical insecticides attract predatory insects and parasitoids?"
Please answer this question based on the informations you knew, either from your research, other people's research, or the appropriate theory.
Mr. Yosua PPA Sianturi
I am currently carrying out an investigation related to the parasitism of Diaphorina citri (vector of the HuangLongBing virus) with the parasitoid Tamarixia radiata (imported) and many people have asked me: Why are you doing it in a greenhouse and not in the field? Which is an understandable doubt given that the final purpose of the research is to release it in the field to exercise its respective control over the psyllid, however, my current answer is: It is necessary to evaluate the parasitism under controlled conditions because the data obtained will be more Accurate, which is necessary because it is an imported agent, if it were native it could be evaluated without problems directly in the field. In addition to that I will be able to determine which are the most favorable conditions (temperature, humidity and light intensity) for the massive rearing of the parasitoid. Finally, research carried out in the laboratory always has specific objectives that in the field would be very difficult to achieve because there are many influencing factors that can alter the data. In my specific case, I am interested in filming the parasitic process in time-lapse and developing a parasitism test according to the nymphal stage of the psyllid. I would like to know what your responses would be to a question like this.
Testing the effect of biopesticides or insecticides on natural enemies under laboratory conditions either it may consider as biosafety or conservation?
We are designing a Y-tube assay involving a parasitoid wasp. So far, the wasps do not seem to be too responsive to the stimulus. What are some factors that could improve the assay?
I am planing an experiment to investigate the dispersion ability of Trichogramma dendrolimi under semi-field conditions. As far as I know Trichogramma uses kairomones for host finding but are visual cues also important? I want to place bait eggs of Dendrolimus pini glued to a cardboard in different distances from the point of release of T. dendrolimi and to check if they are parasitised. I just don't know if the color of the cardboard makes a difference. Is it better to use green (as the eggs and the needles of the trees) or any other color and does the color matter after all?
Tachinidae flies are difficult to identification , so , I need classification key to the species which parasitoid on Lepidoptera>
I need your kind help and guidance to identify these larval parasitoids. These parasitoids emerged from collected larvae of Pinkbollworm, Pectinophora gossypiella in a laboratory. I will be thankful if you guys also help me to provide if possible any supporting document (research article, blog) regarding their proper identification.
Location of collection=Cotton filed of Sindh Pakistan
Maruca vitrata Fabricius, for example, is a major pest of cowpea in Africa. No effective natural enemy has been found for its control. Can your project help to improve the efficiency of some of the known parasitoids, parasites or predators of this insect pest?
Faço taxonomia de importante parasitoides, Braconidae, identificando a nível de gênero. Gostaria de saber das possibilidades de participar neste Projeto.
Dra. Zuleide Alves Ramiro
Hello, any body.
I have data on pesticides effects on Trichogramma chilonis which is a beneficial insect in agroecosystem. The experimentation was completely randomized design. I tried myself but is not succeeded to properly analyze the same as the data is not normal and need normalization before analysis. type I error is common. I have assessed both emergence of parasitoid form the host eggs treated while also evaluated parasitism by the parasitoid. please help me in the data analysis and send me your email address so that I can urgently send you the data for analysis. Your effort will be highly acknowledged. thank you
Dr Muhammad Ashraf Khan
The goal of my project is to develop a biological control method for Dendrolimus pini with Trichogramma spec. So far, I have tried Trichogramma brassicae but it did not work. I would like to try Trichogramma dendrolimi instead but I don't know where to get them. If you are working with them or know someone who does please let me know.
Many thanks in advance,
I reared larvae that came out of a caterpillar body and saw that this wasp did emerge out of the cocoon which the larvae made. The caterpillar is probably a Cnaphalocrocis sp. and it is considered as a pest in Echinochloa polystachya grass. This grass is very important for cattle. The larva of the parasitoid wasp came out when the caterpillar was fully grown and about to pupate. I have observed only one larva that had parasitized a caterpillar. Does this wasp belong to the genus Cotesia (syn. Apanteles)? And what is the species of it?
I would like to know if the content of a group of lepidopteran caterpillars could be sequenced trying to identify only the parasitoids they contain (endo- and exo-). Maybe there are some specific primers for hymenopterans that avoid amplifying the lepidopteran tissue? Any suggestions?
Thanks in advance,
Intéressés par les recherches fondamentales ou appliquées sur les organismes entomophages (arthropodes prédateurs, insectes parasitoïdes, nématodes entomopathogènes, etc) ?
Participez au prochain de Colloque des Entomophagistes (25-29 mai – Antibes-Juan les Pins, France) !
I am an undergraduate and I am starting my first experiment using a parasitoid fly and its moth host. I am currently raising the hosts but will need to run multiple cycles of host growth to get enough eggs for my experiment. Does anyone have a recommendation for tracking multiple sets of insect life cycles? I feel like I need a good system to start with or can miss key windows of either development or parasitization. Thank you for any help you can provide
I think I can remember that I saw a presentation during my studies about the relevance of generalists in hindering insect pest mass gradations. The think explanation was that generalists are not dependent of the population dynamic of the pest insects. So there is a base abundance of the generalists compared to specialized parasitoids, which are not able to end the mass gradation, because their population dynamic is delayed in relation to the host population dynamic. Would be nice to have reference for that issue. Thanks for answering.
> Are there cameras/webcams that can be used to record (continuously) for a whole day or more a small organ of a plant (e.g. stipules) and the visits it gets from small insects like parasitoids?
Thank you for all ideas/advice/suggestions, even if it was for a different insect type.
Bracon hebetor (Parasitoid) is a bio-pesticide for controlling leaf feeding caterpillars. But what number of Bracon hebetor is needed per hectare to control these caterpillars effectively?
I am working on Molecular identification (gene COI) of parasitoids (Chalcid Wasps). After DNA isolation by using Nucleospin kit, Gel check confirmed bands for most of the samples. But, PCR bands are missing as confirmed by gel check. Universal primers have been used.
Hello, I'm planning to assess insecticide resistance of aphid and its parasitoid along with selection. There are many assays I've found in articles so far.
There are leaf dipping assay, feeding insecticide mixed with 10% sugar solution, bial assay(using residue after drying liquid in a bial).
These are the methods using insecticide directly to adult parasitoid.
And some suggested that using insecticide to the infected aphid(developing parasitoid larvae) is better.
I can't decide which method is more appropriate, but I think that contacting insecticide to adult female only make a selection slower.
Any idea how to expose the cocoons with pupae in the wild without being prey to ants or other animals?
Are you aware of scientific works that illustrate the method?
Thanks in advance.
I'm currently undertaking a project that seeks to asses the distribution of a minute gall midge, Arthrocnodax fraxinellus, and its associated parasitoids (Aphanogmus spp.) in Europe.
I'm asking for material of ash cauliflower galls (Aceria fraxinivora) on ash (Fraxinus spp) as the gall midge feeds on the mite in the larval stage.
Material from the following countries are of interest:
Bosnia and Herzegovina
I have attached a PDF with details about the project - please have a look.
Thanks in advance!
I need to mark some aphids in a patch, but that cannot affect their behavior or the behavior of the parasitoid that will attack them (it is a parasitoid-host assay).
Rearing Black Soldier Fly (Hermetia illucens) as future animal feed has to take in account the enemies of the flies.
My studies did not revealed such attacks from parasitoids in my region (Middle East).
Does any one knows parasitoids - in the country of origin for example?
I'm a Master student looking for an interesting applied ecology problem to investigate in my thesis project.
I've been considering to deepen the possible use of parasitoids in fighting invasive macroalgae species but i couldn't find much literature about this. Does anyone know of published papers about the relationship between parasitoids and algae? Thank you very much!
Now I am interesting in if any kind of light wavelength could usefully attract pests natural enemies like lady beetle, predatory bugs, hover fly, lacewing, or parasitoids. Could any one could tell me where could I find related research articles or messages. Thanks for your help!
I am looking for one (or more) populations of T. embryophagum egg parasitoids for carrying out some lab experiments, and was wondering if anyone has a population that they would be willing to share with me?
If the host/prey is only paralyzed (not dead), then their larvae should be considered ectoparasitoids (like those of dryinids, pompilids, scoliids, etc.), shouldn't they?
Contrary to this, only ampulicids, and some sphecid (e.g., Chlorion) and crabronid species (e.g., Larra) are considered parasioids (and, what is more, _secondarily_ parasitoids) among apoid wasps.
I am trying to figure out how many Myzus persicae would infest a potato without top-down control (predator or parasitoid). In some papers, I have read that Myzus persicae is non-gregarious and therefore does not occur in a large number in a host plant. However, from my experience, a tobacco plant could hold quite a number of Myzus persicae and so could a chinese cabbage. Am I wrong to assume that there will be hundreds of aphids when there is no interference? Is Myzus persicae always gregarious or does it depend on the host plant it feeds on?
I am trying to assess the efficacy of hymenopteran parasitoids as biological control agents of cutworms, but they have very low parasitism.
I obtained three parasitoids from the same batch of eggs from a Reduviidae bug from Colombia possibly from the Platygastridae family. There are few published records of parasitoids in Reduviidae eggs. I would like to know if anyone knows what genus and species are? The yellow individual may be an hyperparasitoid. If you have published records please let me know.
Full reference: Graham, M.W.R. de V. (1991), A reclassification of the European Tetrastichinae (Hymenoptera: Eulophidae): revision of the remaining genera. Memoirs of the American Entomological Institute No 49, 322pp
We are looking for an entomologist / taxonomist who is willing to identify this Phytomiptera species (Diptera: Tachinidae) that we encounter in the Bolivian Altiplano.
This Phytomiptera is an endemic parasitoid of Eurysacca quinoae (Gelechiidae). Eurysacca larvae cause damage in quinoa crop cultivation.
Since PROINPA is a Bolivian NGO, we are not able to pay you for this job. But of course the scientific credits if it turns out to be a new species are yours!
(Dead samples send upon request)
We are looking for an entomologist / taxonomist who is willing to identify this Meteorus species (Braconidae) that we encounter in the Bolivian Altiplano.
This Meteorus is an endemic parasitoid of Eurysacca quinoae (Gelechiidae). Eurysacca larvae cause damage in quinoa crop cultivation.
Since PROINPA is a NGO, we are not able to pay you for this job. But of course the scientific credits for discovering a new species are yours!
(Dead samples send upon request)
I am looking for anyone who work with Apanteles taragamae, natural enemy of legume pod borer Maruca vitrata. This species is solitary parasitoid. However, I am working with this parasitoid as natural enemy of Diaphania indica, and this is gregarious species. I am interest to make a further identification of these species.
If anyone have a some suggestion, I am mostly grateful for it. Thanks.
Sampled in Greece, Athens, Tatoi
plant: Quercus coccifera
caterpillar: Lymantria dispar
primary parasitoid: Cotesia melanoscela
Thanks a lot in advance
The pea aphid (Acyrthosiphon pisum) has a strong sexual dimorphism with males being thinner than the females. When we used the parasitoid Aphidius ervi, we observed few attack behaviour and, for now, no mummy formation on male aphids. Are you aware of publications on parasitoid attacks on different sexual / asexual morphs of aphids?
Kindly help me to identify these larval parasitoids of Pink boll worm collected from cotton field of Sindh, Pakistan. These parasitoids emerged from larvae in laboratory condition in large quantity and their existence realized at the end of cotton harvesting stage
We compared the effects of a sublethal biopeseticide (after applied LC 50 value) on different biological parameters (time of development, length of life, parasitism ...) of the three different populations of parasitoids Encarsia formosa (two local and one commercialized). For each monitored parameter, we performed a separated experiment, at the same time with all three of the observed population, with and without the effects of pesticides (control courts were sprayed with distilled water only.
Is it right the results processed using two-way ANOVA or is there a more appropriate analysis of the data ???
We want to determine 1) whether the origin of the different populations, 2) whether the pesticide and 3) and 3) whether the interaction of the origin of the population and insecticide affects the obtained values of parameters ???).
Thanks in advance…
All plants respond to herbivore-inflicted damage with the enhanced emission of volatile organic compounds (VOCs), and plants in numerous taxa also respond with the secretion of extrafloral nectar (EFN) . Both VOCs and EFN attract adult parasitoids and predators (hereinafter collectively termed ‘carnivores’), an effect that can significantly reduce herbivore pressure on wild plants . Nevertheless, relatively few attempts have made conscious use of VOCs or EFN for biological pest control and, to the best of our knowledge, classical breeding has never aimed to improve anti-herbivore defense via VOCs or EFN 1 for the first attempt to genetically engineer wheat (Triticum aestivum) . How and to what degree these traits can be optimized to allow better biological control of pests and crop diseases?
Predators are mainly free-living species that directly consume a large number of prey during their whole lifetime.
Parasitoids lay their eggs on or in the body of an insect host, which is then used as a food for developing larvae. The host is ultimately killed. Most insect parasitoids are wasps or flies, and may have a very narrow host range.
Pathogenic micro-organisms include bacteria, fungi, and viruses. They kill or debilitate their host and are relatively host-specific. Various microbial insect diseases occur naturally, but may also be used as biological pesticides. When naturally occurring, these outbreaks are density-dependent in that they generally only occur as insect populations become denser.
It is a study on cider-apple orchards in NW Spain. I have already identify the most abundant species in our region (Ascogaster quadridentata, Trichoma enecator, Pristomerus vulnerator and Liotryphon caudatus ) but we have got a few specimens of apparently new five species. I could provide pictures.
I'm working on one of parasitoid wasps of Anarsia lineatella and I want to rearing eggs on artificial food so I need more eggs for my experiment. I want to know how can encourage females to lay more eggs. I reared both male and females in plastic bags (30*20*10 cm) with peach fruit at ( 16:8 D: L, 23-25°C) but their egg's laying is low. I will very appreciate if tell me your answers
We are looking for an entomologist / taxonomist who is willing to identify this Cotesia species (Braconidae) that we encounter in the Bolivian Altiplano.
This Cotesia is an endemic parasitoid of Eurysacca quinoae (Gelechiidae). Eurysacca larvae cause damage in quinoa crop cultivation.
Since PROINPA is a NGO, we are not able to pay you for this job. But of course the scientific credits for discovering a new species are yours!
(Dead samples send upon request)
I am interested on the drivers of parasitoid dispersion and their impact on biological control. For example, how ecological infrastructures may affect spatial dispersion of parasitoids? Parasitoids are supposed to disperse based on short flights. Therefore, the diversity and structure of plant communities are expected to influence their dispersion pattern, and consequently their impact on insect pests, as biocontrol agents.
even the weather that can modify the sustances like pheromones, what are now the reasons for that succesful development like beig a parasitoid.
I have been working on gall morphology in phylloxerids and it got me thinking about their parasitoids. I know that some wasps can manage to oviposit through aphid galls, but I don't know any parasitoids that are known to attack galls of Phylloxeridae (probably because we know almost nothing about that family's ecology, Grape phylloxera aside).
Has anyone ever encountered parasitoids of phylloxerids in the field or know of a paper on the subject? I really appreciate any help you can provide!
I'm working on taxonomy of European Hymenoptera at family level and I'm finding difficulties to distinguish between gall wasps (Cynipidae) and small parasitoid wasps (Mymaridae, Chalcidae, Eulophidae, Pteromalidae) with the resources I have available. I need better identification keys/guides. Thanks in advance!
Chrysoperla is a generalist predator of many insect pests, however the eggs are parasitized by several species of microhymenopteran wasps like the one in the photograph. I wonder the impact of these egg parasitoids in the predatory efectiveness of Chrysoperla to controlling insect pests. Any idea about the ID of this parasitoid from Colombia ? thank you.
I have just started field study on it with Trichogramma application. I want to know could the wasps achieve good control effect on controling tiny eggs like small budworms of apple and pear?
Any idea what kind of larva this is?
It comes from a willow catkin and is about 7 mm.
A family name would already be great. Can it be a parasitoid wasp larva? Does it attack Diptera larvae?
I want to rear Sitobion avenae, its predators (ladybird beetle and green lacewing) and parasitoid (Aphidius gifuensis) in the rearing room. But I am confused about the requirements of temperature and relative humidity in room. Kindly tell me at which temperature and RH, I reared these insects?
I am moving single trichogramma around (for sexing, mother-son mating, experiments etc), and have experimented with just waiting for them to climb up small tubes (but then it can be hard to get them out again), using an eyelash, or an aspirator (but this is proving not optimal, the one I made anyway)... what is everyones experiences, and anyone found an effective, and time-saving, technique?
Many thanks in advance!