Questions related to Parasitic Diseases
I am part of a group exploring a potentially novel infectious organism. We are working to identify it by microscopy images and genetic sequencing. We would greatly appreciate expert opinions on some of our images. Please see our project at: https://www.researchgate.net/project/Characterization-of-possible-novel-zoonotic-eukaryote
Thoughts, comments, questions? Greatly appreciated.
Plasmodium, zoospore, oomycete, myxamoeba, apicoplexa, infectious, parasite, disease
What are the possible negative effects of the low-dosage drug on the people who drink the water? Have there any been studies that look at its effectiveness?
Nobody had so far thought of developing a vaccine against the disease. And for good reason: unlike mammals, insects do not make antibodies. The latter, however, are central to the principle of vaccination, which involves introducing an attenuated or modified strain of a microbe so that lymphocytes learn to recognize it
I have some 2 drugs that I want to determine their nature of interaction against a tropical disease parasite. I need to plot an isobologram to achieve this. How do I go about doing this?
It seems that original wild plants are more resistant to diseases and parasites that those selected and "improved" for their commercial value
Do factors such as temperature and pH play a role in dictating the prevalence of S. japonicum?
Parents of a young kid come up with a complaint of passing many worms in the stool (The picture of the parasite is provided below). The parasite was eradicated with Praziquantel treatment, however, initial treatment with Albendazole didn't work well. Could anyone help in identification of this parasite?
Since the treatment of cancer patients can reduce their immunity to diseases so it is advisable to screen them for some parasitic diseases that might be very harmful for them such as toxoplasmosis and cyptosporidiosis
The COPD is freqent. Parasitic-infestatio is even more and causes blood-eosinophilia. How can be predictiv the count of eo on COPD exacerbatio-phenotyp without exclusion of the helminthiosis or other parasitic disease?
I submitted my manuscript more than 1 year ago. After initial processing of it where editors were asking me a documents and some editing corrections, than everything just stopped. I repetedly ask explanation and editors are barely replying to me. Once every couple of months, average, reply asking me the same papers and the same corrections.
Have you evere faced this situation? I would like to have at least my manuscript rejected in order to submit elsewhere.
What should I do ?
Please any one can provide me a sequence of specific primer of Theileria camelensis which infect camels or any related method for molecular identification of this protozoan parasite.
I conducted a retrospective assessment of real-world treatment of a parasitic disease without standard tx schedule. 7 different schedules were used. Half cases (230) received one treatment. Looking for someone with experience in propensity scores to participate in the comparison of these treatments.
We all know that vaccines for many important viral and bacterial diseases are produced successfully . while this is not occur in case of parasitic diseases . can my collegues tell me why?
I have problem with tick molecular identification. Can anyone send me the Papers of species specific primers sequence (Either ITS2/COX1 or 16SrDNA) used for Hyalomma marginatum and Hyalomma truncatum? Pls send to my email address firstname.lastname@example.org. I will be very thankful to you for this
As we know some types of parasitic infections can be detected by testing blood through serology or blood smear. Few studies, however, suggested to detect the DNA of Echinococcus granulosus or Echinococcus multiloculris from blood. Why not?
Thanks for helps in advance
What are the companies or government bodies in India who can provide international standards for Hepatitis B, C, and HIV which can be used for quantitiative measurement using Real -Time PCR .
I need to determine infection rate of dedritic cells by Trypanosoma cruzi determining amastigotes forms insides these cells and I have thought to do that by RT-qPCR to detect some specific transcript or protein that only this life cycle can produce, since count the infected cells and the amastigotes inside the cell is not a accurate method.
Or are there another way more accurate to determine infection rate inside the cells if it is a intracellular parasite?
CE is found in Africa, Europe, Asia, the Middle East, and Central and South America. Highest prevalence is found in populations that raise sheep. In North America, Echinococcus granulosus is rarely reported in Canada and Alaska, and a few human cases have also been reported in Arizona and New Mexico.
Since S.japonicum lives out its mature adult stage within humans, it would be insightful to know whether the parasite thrives more in those with lowered immune systems or those who have more nutrients available to them.
I start a project about the prevalence of GIT nematodes in horses reared in middle province of Iraq , I need the most accurate method for identification .
A genus of coccidian parasites (family Haemogregarinidae), in which schizogony occurs in the visceral organs, gametogony in the leukocytes or erythrocytes of vertebrate animals, and sporogony in certain ticks and other blood-sucking invertebrates.
I have read that control measures targeting the molluscan intermediate host Oncomelania quadrasi present in the Philippines were not as effective as predicted. In light of this, I was wondering if controlling S. japonicum itself would be preferable, and if there are any methods that have already been employed and proven effective.
What is the best statistical analysis for the reinfection of schistosomiasis, given that the data consists of the EPG (eggs per gram) of stool samples of 3 sets of different host species?
For instance, if the climate is warming, can we expect that vector-borne parasitic diseases of the tropics will invade more developed countries.
Which medium is best to culture the albugo candida? although it is obligate parasite... how can we inoculate to produces disease on crucifers?
How do you coat an antibody on a nitrocellulose membrane in order to develop immunology parasite antigens diagnostic strips?
Hi please can any one help me with my study about periodontitis , molecular study ,identification of Anaerococcus prevoti , primer
Diagnosing Giardia could be challenging using conventional parasitological techniques. I looked for commercial kits for Giardia diganose, but all of them are indicated for G. lamblia. Does anyone ever tested them with wild animals?
Sarcocystosis is an uncommonly reported parasitic infection which has not been listed as an opportunistic infection as infections caused by other coccidia (Cryptosporidium, Cystoisospora). Epidemiology and clinical impact of human infections by Sarcocystis are largely unknown and reports continue to be rare.
Unlike other coccidia, sarcocystosis is not considered an opportunistic infection and evidence linking sarcocystosis with AIDS is limited with only three cases published globally:
I am interested in knowing of other reports (even not officially published resources) of Sarcocystis infections in immunocompromised patients. Thanks
As not all species of cuckoo are brood parasites, is this just an evolutionary adaption within some species? is it down to relative brain size? or a possible learnt behavior ? are we in a current process of evolutionary change
I want a research or idea about the larvae of Toxocara canis if can attenuated and used it as a vaccines against this parasite infection in human and dogs
Is there any reference that shows that a Leishmania infected sandfly when bites to a mammalian host could not lead to successful infection. This may be because at that time the vector may be carrying the pool of parasites majorly with the avirulent ones. Because different morphological forms have different virulence.
I continue to work with mammary infections by helminths in a variety of hosts. This has been shown extensively for some nematodes, especially strongyloids, in some hosts and in some parts of the world. Fewer cases of such infections have been described for cestodes and trematodes, though some exist. I am interested in further collaboration on such possibilities.
Which species of Cryptosporidium causes gastric cryptosporidiosis apart frm C. muris and C. andersoni (These two I know) and which cause intestinal cryptosporidiosis?
In picture 20160915_2 the size of egg (C) is compared to an egg of roundworm (A) and of whipworm (B). Picture 20160915_3 is the oval shaped egg with operculum.
Leishmania exhibiting a fatter morphology than expected for their growth phase. Suspected change in osmolarity caused by differential prep of some M199 reagents. Any thoughts?
Parasites can be reduced to less parasite count in human body and patient be clear from symptoms , However after couple months or a year of being treated the patient is infected again due to Bio-films.
Without mechanical cleanse and blood analysis checks and following up infection comes back.
How can we destroy those bio-films and prevent re-occurrence of infection?
I am looking for information on the mite's life cycle in order to determine if we can trial some pasture management schemes to minimize reinfection.
Zoonotic rickettsial diseases are acute specific febrile illness caused by rickettsia, small bacteria like m.o. transmitted to humans by arthropod vectors such as tick, lice, mite and fleas. Rickettsioses of zoonotic importance can be divided into groups based on characteristics such as their intracellular locations, antigenic properties, DNA composition and the diseases they cause.
Some body transfect the T. gondii tachyzoites using complicate method/s I am looking for a simple and more applicable method for transfection of siRNA to this protozoa. Is there any experience?
I wish to carry out a study on pinworm infection among children. I will be thankful for your suggestions on:
1. Features suggestive of pinworm or worm infestation.
2. Features increase the risk of pinworm infection.
3. Knowledge aspects need to be assessed from parents or guardians
4. Sites from which swab should be taken for pinworm eggs in the environment
Please respond to the attached delphi survey.
Hello, i am researching about Plasmodium knowlesi. i have read this paper
The paper still using Pmk8 and Pmkr9 to detect P. knowlesi.
But i still wondering if P. knowlesi could have a co-infection with another malaria parasit for example P. vivax.
Can someone provide me a study that confirm a P. knowlesi co-infection using Primer used by Imwong et al 2009? http://www.ncbi.nlm.nih.gov/pubmed/19812279
we have came across a sample of sputum full of larvae .we are having difficulty in diagnosing whether it is larvae of strongyloided/ancylostoma or ascaris. the theory books and images in books and net are some what confusing .
The companies address and the email of professors would be good.
I would like to conduct DNA barcoding of parasites from a stool sample of a monkey. I don't know if there are protocols for extract parasitic DNA from stool sample.
Has there ever been documented cross reactivity between antibodies to Plasmodium vivax AMA-1 or MSP-1 antigens and other malaria parasites or other pathogens in peer reviewed literature?
I have isolated human neutrophils (from blood) in the inactivated condition for parasite infection study. I need to fix the cells for staining protocol. I have used 1% and 4% paraformaldehyde. I am getting more ghost cells during staining protocol. What should I use for fixing cells? Also I need it to grow on coverslip. What is the method used for adhering neutrophils on glass coverslip?
I'm interested in teaching Parasitic diseases to students of veterinary medicine using CLIL. I'm grateful for any help, first-hand experience, notice on the topic, guide to a web site etc.
I have a question about accidental leishmaniasis !
The Last day I was working on my recent experiment on a new vaccine against cutaneous leishmaniasis and unfortunately I do all of my research steps lonely ,thus , when I was injecting live stationary pathogenic leishmania major to foot pad of BALB/c mice suddenly some drops of parasites suspension Sprinkled from the syringe to my eye!
I washed my eye immediately, but I want to know that I can take ocular leishmaniasis!!
It seems impossible because the eye environment condition is not suitable for this parasite, but, I want to know your opinion.
I found many article about ocular leishmaniasis as a result of visceral leishmaniasis or cutaneous leishmaniasis like as follow:
M ModarresZadeh, K Manshai, M Shaddel…-Iranian Journal of Ophthalmology , 2007
*Simultaneous occurrence of ocular, disseminated mucocutaneous, and multivisceral involvement of leishmaniasis.
Philips CA, Kalal CR, Kumar KN, Bihari C, Sarin SK.
Case Rep Infect Dis. 2014;2014:837625. doi: 10.1155/2014/837625. Epub 2014 Feb 18.
*Leishmaniasis of the eyelid mimicking an infundibular cyst and review of the literature on ocular leishmaniasis.
Veraldi S, Bottini S, Currò N, Gianotti R.
Int J Infect Dis. 2010 Sep;14 Suppl 3:e230-2. doi: 10.1016/j.ijid.2009.07.024. Epub 2009 Dec 6. Review.
*[Ocular leishmaniasis in a cat: case report].
J Fr Ophtalmol. 2013 Apr;36(4):e67-72. doi: 10.1016/j.jfo.2012.09.006. Epub 2013 Mar 1. French.
and many other.
Thanks for your comments.
I am carrying out a research project to investigate the dominant circulating variants of the pfAMA1 gene in a population. I have already extracted the plasmodium falciparum gene but need to carry out PCR and sequencing to identify frequency of the different variants. I however need to know the region which exhibits the 3 variants and the length in base pairs so as to perform nested PCR and also determine the cost of sequencing according to the number of base pairs thus preventing wastage
Toddy (Palmyrah wine/palm wine) is said to be the culprit of hepatic amoebiasis in Northern sri lanka. But nobody has isolated amoeba from palm wine.
Now, I plan to design my experiment about hookworm parasite infection. So, I want to know what i should do in blood test for hookworm infection in golden hamster. I saw in many publications that used a measuring hemoglobin level only. Why do the CBC and HCT blood test is not use?
Thank you for your kind response