Ovary - Science topic
The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE.
Questions related to Ovary
The supplier of the HTB-78 line (ovary adenocarcinoma, adherent) recommends culturing on Leibovitz's L-15 Medium in 100% air. Maybe any of You tried to grow on a different medium (DMEM?) in an atmosphere with the addition of 5% CO2?
I will be grateful for all the hints!
So I desperately need to remove all traces of genomic DNA from my RNA samples. I have tried, what I think are all the possibilities, to remove the DNA, so far without success... My RT - always amplifies.
Here are the different technics I have tried thus far:
- 30 ovaries RNA extraction, Turbo DNase treatment on the QIAGEN RNeasy column
- 30 ovaries RNA extraction, Turbo DNase treatment on the QIAGEN RNeasy column + TurboDNase free rigorous treatment 1uL of DNase
- 30 ovaries RNA extraction, Turbo DNase treatment on the QIAGEN RNeasy column + TurboDNase free rigorous treatment 3uL of DNase
- 30 ovaries RNA extraction, Turbo DNase treatment on the QIAGEN RNeasy column + dilution of cDNA 1/50 + TurboDNase free rigorous treatment 2uL of DNase
- 30 ovaries TRIZOL-Chloroform RNA precipitation (no kit) + TurboDNase free rigorous treatment 2uL of DNase
- 30 ovaries TRIZOL-Chloroform RNA precipitation (no kit) + dilution of cDNA 1/50 + TurboDNase free rigorous treatment 2uL of DNase
- 10 ovaries RNA extraction, Turbo DNase treatment on the QIAGEN RNeasy column
Thanks for any help!
In the discovery of mammalian egg von Baer reported to have seen a yellow fleck inside the follicles; this could correspond to sighting- with the microscopes of that time- to the cumulus o-hutus, which convex face in the antrum could be described as a fleck or point?
I am asking about autophagy inducers and inhibitors for use in mammalian ovaries in vitro as well as in vivo condition.
Our long standing understanding for mammalian ovary is that it has fixed number of germ cells and duty of the ovary is to ovulate high quality ovum for successful fertilization and early embryonic development. However, growing studies suggest that the oocytes can be generated from Oocyte like stem cell/neo-oogenesis/adult oogenesis and fertilized in vitro. Therefore, it is important tom know whether oocytes generated from these sources are as good as the follicular oocytes collected from mammals under natural conditions. Kindly give your opinion on the genomics, proteomics and metabolomics aspects of ocytes generated from Oocyte like stem cell/neo-oogenesis/adult oogenesis.
Hello - I need to retrieve GV stage oocytes from mice ovaries. I do not know where to get the small tool that is used to mush the ovaries into small pieces. I do not know how to fabricate it either. Does anyone know if it is commercially available? If not, do you have any spares you are willing to part with? Thank you very much in advance.
I transfected the CHO (Chinese hamster ovary) Cells with Plasmid containg Heavy and light chai genes of a mAb, I'ld like to know during which phase of cell growth the mAb will be produced ?
I have preserved my tissue sample (ovary and follicles) in RNA Later and stored at -80 degree celsius. I have tried to extract the RNA using Trizol reagent. I have even washed the tissue with 1X PBS but still the RNA is degraded. I need an advice on this.
We would like to investigate the effects of a pesticide on ovary. But if we treated with single dose and take the samples at the 24 hour can we see significant changes at the histology and ultrastructure? Can we evaluate apoptosis and oxidative stress parameters at the 24 hour of pesticide exposure?
The combined oral contraceptive pill is often just called "the pill". It contains artificial versions of female hormones oestrogen and progesterone, which are produced naturally in the ovaries. If sperm reaches an egg (ovum), pregnancy can happen.
I have done H&E staining of uterus and ovaries of rats, now want to do scoring of the lesions in percentage%, any idea which technique or software I can use??
The PGF2α transferred from the uterus to the ovary is thought to occur either by local countercurrent transfer or general systemic transfer. Countercurrent transfer involves the movement of molecules (PGF2α) across the blood vascular system from higher concentrations in the venous effluent (utero-ovarian vein) to an area of lower concentration (ovarian artery). Systemic transfer involves the passage of the molecules through the general circulatory system.
In some species (cow and ewe), PGF2α synthesis from a uterine horn only influences the life span of the CL in the ipsilateral ovary. In other species (sow and perhaps mare), PGF2α synthesis from one horn is sufficient to cause regression of CL in both ovaries.
Can the consumption of extra fat (2%) in the diet of prepubertal gilts cause an increase only in the weight of the ovaries? The rest of the uterine structures are not affected (no CL, CA..)
Why does this occur?
Is there any in vitro model for Cystic Ovarian Disease available to study the efficacy of various therapeutic options in vitro before it is implemented in COD condition in cows/bovines?
See the image attached below and give me few valuable suggestions regarding gonad intersex. The below image showing 9 months old male zebrafish dissected out viscera to check the gonad status. The fish was exposed to pesticide (secret) at the age of 7 months old. Looking forward to your valuable suggestions.
I am working on toxicity induced by drugs on invivo model(mice) and few compounds that have antioxidant properties that would be used to reduce this toxicity. The organs I would be looking for would be liver, kidney, ovaries, brain, heart. My question is to study antioxidant profile, what would be better to analyse antioxidant enzymes Serum or tissues?
I want to do sample digestion for AAS or ICP-MS manually to detect some heavy metals, i have uterus and ovaries of rat model, any idea how it can be performed.
I have extracted total RNA of rat ovaries and uterus by TRIzol reagent. The integrity and purity of extracted RNA was suitable. The cDNA was made using an one step commercial kit. Now, I have problem with RT process , as the received CT is about 36, even for my house kipping gene. I mixed 1 micro-liter of each primer, 3 of cDNA, 10 of cyber green, and 5 of DEPC-treated water. Can anyone tell me what should i do?
Hello, I've been recently trying to standardize the DCFH method for ovaries.
According to the stablished method, ovaries recently dissected have to be incubated immediatly, embeded in tissue-tek, processed on the cryostat and observed by fluorescent microscopy.
Unfortunately the amount of samples I get in a day take many hours to process and I wanted to ask if it was possible to incubate the ovaries with DCFH and store it in tissue tek in a dark place so I could analyze it later or if I could store at -80°C and then incubate the ovary with the DCFH
I haven't found if this is possible or if HAS to be with fresh samples.
Hi, I have been using ImageJ to measure the area of fetal ovaries, with Cleaved Caspase 3 and mvh/ddx4 expression for my IF. mvh is the green channel and CC3 is on the red channel.
I haven't had a problem measuring the area of expression of both of these individually (Colour>Split Channels>Adjust>Threshold>Create Selection>Measure, after setting scale and setting measurement as area).
What I need to know is, is there a way to measure the area that is expressing both red and green simultaneously? So that I can quantify the total area +ve expression of germ cell marker and cc3 in the ovary?
n.b. I have also counted the individual number of cells, but I don't think it is possible to manually count those that are CC3+, hense turning to area and density.
The protocol using 4% PFA as fixative and subsequently 0.5%Triton X-100 as permeabilizing agent is standardized in the lab and we are able to stain for most other proteins. The antibody for that protein works well for other tissues like ovary. Has anyone faced similar difficulties while staining for membrane proteins and could maybe suggest any changes in protocol?
What is the best chemical in inducing PCO? is Letrozole or Estradiol-Valerate EV?
How can I get non-time consuming method in PCO induction? and what are the doses?
We are going to house mice for a double-ko investigation. Since this is our first time housing mice, we have no knowledge on how we should optimize breeding.
So here's the info:
One gene is on Chr-X, the other on Chr-11. Both males and females homozygous double-ko should be infertile. The simple ko are supposed to be ok. We are mostly interested in ovaries of the double-ko females, but we'll also check on both simple-ko and wt as controls.
So what strains should we ask to start the colony ? We also want to cryopreserve embryo in the first few weeks.
The collaborator who is going to send the mice proposed this:
Males: Gene1(-/0), Gene2(-/+)
Females: Gene1(-/+), Gene2(-/+)
Do you think that is an appropriate start ?
I am struggling to find a way to measure oocytes on histological sections with the nucleus while excluding those without the nucleus. Since the measures would involve the whole histological section (i.e. a few hundreds of oocytes to manually be measured) I am trying segmenting and/or thresholding the pictures on imageJ, but without success. The idea underlying this approach is to find a way to automatically detect the "objects" I am specifically interested in.
I've already tried ImageJ Trainable Weka Segmentation and MorphoLibJ.
Here a couple of pictures of ovaries DAPi-stained and what I'd roughly like to obtain. Red and yellow circles for oocytes with and without nucleus, respectively.
Thank you in advance.
Is it recommended to evaluate the estrogenic potential of a plant extract in female mice without ovariectomy, if we keep a control group without the administration of the extract? Because normally the estrogenic activity is checked in ovariectomized female mice. But if we found the extract efficient, we would be providing it to a female with ovary.
My project is on ovarian biology and to design experiment, I want to know how the in vivo testing could be done
As we all, in the field of animal reproduction know that the main response for superovulation in animals is determined by the number of increased numbers and diameters of ovarian follicles. Do we can find out a lab method to assess number of granulosa cells that respond to the external dose of FSH treatment (i.e. correlation between dose of FSH and number of responsive GCs).
I am looking for dubious gene(s) that may causing ovary polycystics?? Some genes are highly expressed in people that are tolerating polycystic ovary or infertility.
Im aspirating the follicular fluid from surface follicles of buffalo ovary, centrifuge the fluid and pass it through 40 micron followed by 20 micron and then by 0.22 micron. But 0.22 micron filter get stucked after filtering just 1 ml. Can any one guide me to filter FF easily and in large amount in single go.
I followed Brown- peterson et al, (2011) and atlas of fish histology to classify the maturity stages of ovary and testes of Caspian goby but i am not certain about the type of cells and stages on light micro graphs of Caspian goby testicular and ovarian histology, for example in spawning capable phase for males , two sub phase are recognized by me that are mid and late GE (following pictures) but i do not know it is correct or not
Australia's National Health and Medical Research Council (NHMRC) produced a sham review of Water Fluoridation in 2017 by deliberately ignoring over 3000 peer-reviewed scientific papers on Fluoride Toxicology. One important paper the NHMRC suppressed dealt with the mechanism by which Fluoride damages your teeth, with or without metal ions like Aluminium. Fluoride causes increased SATB1, a factor associated with Malignant Cancers and their Metastasis, with many relevant publications relating to Leukemia, Melanoma, Laryngeal and Nasopharyngeal squamous cell carcinoma, cancers of the Bladder, Breast, Cervix, Colon, Kidney, Lung, Ovary, Prostate, Uterus, and Liver.
A couple of papers: Zhang Y, Kim JY, Horst O, Nakano Y, Zhu L, Radlanski RJ, Ho S, Den Besten PK - "Fluorosed mouse ameloblasts have increased SATB1 retention and Gαq activity" PLoS One 9(8):e103994
Fluoride interferes with FoxP3
Zhang G, Zhou B, Han T, Wang M, Du X, Li Q, Wang J. 2012. Decreased percentages of CD4+CD25+ regulatory t cells and foxp3 expression in the spleen of female mice exposed to fluoride. Fluoride 45(4)357-364.
Observed increases in cancer incidence over four decades follow the roll out of Fluoridation and increased Dental Fluorosis. Surely it is time to ban Fluoridation worldwide?
I am trying to purify protein from squid ovary (Moisture content, 75%; protein content 19%). However, due to high amount of carbohydrates (8%) it hindering purification in the first step ammonium sulfate precipitation (unable to sediment the protein).
Therefore, I want to separate carbohydrate and proteins from my sample.
Mostly precipitation methods, using solvents like ethanol/acetone has been used to separate these component but functionality of one of the component is affected negatively.
Is there any other method used for separation of carbohydrates and proteins without affecting functionality of both components .
Please give me valuable comments/suggestions to sought out this problem.
Thanks and regards
I just want to check the expression of a particular mRNA throughout the different tissues (Liver, Kidney, Brain, Muscle, Gills, Intestine, Ovary, Skin, Spleen etc.) in a control/untreated fish. If I go through the Real-time PCR taking all the tissues together, then what method should I follow to meet my quiry?
As you know poly cystic ovaries and infertility is difficult to control in many cases. Sometimes prescribing traditional hormonal drugs and metformin in over weight female with NIDDM is not effective. What is the best therapeutic agents to control these patients?
I am attempting to isolate ovary from Helicoverpa armigera larvae. I can't find any pictures showing what it looks like during dissection. I know it is possible since there are several articles describing sequencing of gonad transcripts (ovary and testes). Any help? Ideally I would like to see some pictures of what the ovary looks like during dissection and where exactly it is located.
Thanks in advance!
EDIT: Thanks to Muhammad F Chaudhury for providing me the full version of his article titled " Spermatogenesis and Testicular Development of the European Corn Borer, Ostrinia nubilalis (Lepidoptera: Pyraustidae)". In it he describes that in this insect the testis of late-instar larvae are yellow-orange colored, reniform and aprox. 1mm in size.
This information allowed me to identify them in Helicoverpa larvae.
We took in collage that about 14 follicle undergoes follicule phase each month but only follicle which become large than all and due to its secretion of estrogen it, decrease the secreation of fsh but it increase also the no. Of fsh receptor so the it makes intrinsic postive feedback in this large follicle., so the the rest of small follicles will degenerate and only this large follicle will be able to survive(thug life awii), but if that was really the reason for the the ovaries alterntnation in ovum secreaction., why cant one ovary secrete an ovum in 2 successive months?, this may be due to Corpus albican which remains for months till it degenerates (so it. Basically occupy space allowing the other ovary to produce the next month ovum), I really dont know if I am right or wrong, and I havenot searched alot, so if anyone have the answer please till me 😀😀😀
i am isolating EV from culture emdium of amniotic fluid stem cells amd injecting them into damaged ovaries of rats . i was wondering if anyone has tried injecting them into the tail vein and yielded results.
Patient is married from last 5 years and has a child. Now she has difficulty in conceiving the second child. She is on Metformin 500mg twice a day.
we want to detect alpha estrogen receptor in ovary and uterus of rat with IHC method. But in the result, the negative control stil stained and it he granulosa cell was not stained.
I want to isolate RNA from 15,5-17,5 dpc ovaries (preferably with Trizol).
How many ovaries I should add in tubes with Trizol? Should I modify the protocol?
The goal is to quantify oocyte-specific genes by qPCR.
A 28 years old lady, height 5 feet, weight - 70 kg has been trying to conceive for the last on and a half years. She has a 32-33 days' menstrual cycle. Husband's semen analysis, follicular study and HSG reports are normal. Ultrasound of pelvis reveals evidence of polycystic ovaries with normal sized one ovary and other of 11 cc. She has been put on Tab Metformin 500 mg three times a day and Chirocyst by another Gynaecologist. She gives H/O partial seizures and is on Tab Orcabezapine. The patient uses Ovulation predictor kit to time intercourse. She used it on 14th and 16th day of the present cycle. The menstrual period got overdue by 6 days, yesterday. The patient used the Ovulation predictor kit and the result was positive. Today, she had a normal period. This is the first time that she had a delayed period. The query is, can ovulation predictor kit show positive result just one day prior to the onset of the menstrual period? Do the drugs she is taking interfere with LH levels?
Our research group at Komar University for Science and Technology looking for a suitable compound/chemical to induce cystic ovaries in the rat model, and then treat them with a natural product of our country to see the effect and outcomes.
Now, we need a chemical with fewer side effects to induce cystic ovaries. TQ
I am extracting carotenoid pigments from animal tissues like ovary, hepatopancreas etc. of prawn using solvent system Methanol and Dichloromethane. After extraction, can I directly go for HPLC for quantitative analysis or should I need to perform TLC for initial purification?
usg shows undetectable to very small uterus with ovaries not clearly visualized with associated blindness.
Dear all, according to Grimes DA et al., 2014 ( http://onlinelibrary.wiley.com/doi/10.1002/14651858.CD006134.pub5/abstract;jsessionid=B22DED607EDF16AB77AF3505560F19B1.f04t02 ), treatment of functional ovarian cysts (FOC) with OCP appears no better than watchful waiting. But it's evident that some progestagens (I guess it especially may be refered to 19-norsteroids) have a definite anti-gonadotropic effect. What about this? Does anybody have a reliable information about the positive influence of some OCP or progestagens on FOC? Thanks a lot!
We obtained several preliminary images from a electron microscope. The sample is Chinese hamster ovary cells being treated by freeze-fracture technique and certain proteins in the membrane are labeled with gold particles (10nm). But we are not really sure if the image is showing a classical mammalian cell membrane surface or not. ：I
So hopefully any expert could help me confirm if this is really a mammalian cell membrane. Thanks a lot !
I'm new at this so I would appreciate any protocol for western blot in order to determine ATG7 and LC3I/II in rat ovary. Thanks in advance.
CHO-Lec1 cells have a defective N-acetylglucosaminyltransferase gene or it completely lacks it. From the original papers it seems like they have a reduced enzyme activity due to mutations. But in some places I also came across statement that they lack the enzyme (N acetylglucosaminyltransferase )
Stanley P, et al. Selection and characterization of eight phenotypically distinct lines of lectin-resistant Chinese hamster ovary cells. Cell 6: 121-128, 1975. PubMed: 1182798
Stanley P, et al. Chinese hamster ovary cells selected for resistance to the cytotoxicity of phytohemagglutinin are deficient in a UDP-N-acetylglucosamine-- glycoprotein N-acetylglucosaminyltransferase activity. Proc. Natl. Acad. Sci. USA 72: 3323-3327, 1975. PubMed: 1059116
I am working on the effect of toxicants on the ovarian production of steroid hormones. Please, can someone recommend the most suitable cell line to be used as a model for this assay?
Pregnant 21 year old had IA mucinous ovary cancer at week 14th of pregnancy and underwent to oophorectomy + omentectomy+ peritonial biopsy + appendectomy due to her desire to lead pregnancy to term and have baby.
Pregnancy follows with no complications up to now and on February 25th she'll be 39,1 weeks and it will performed a cesarian section.
Our question is about cancer approuch. Is better patient clinical follow or radical surgery at the same moment of cesarian section?
i want to know the equipment to determine that and the methodology for the estimation