Science topic
Orchidaceae - Science topic
A plant family of the order Orchidales, subclass Liliidae, class Liliopsida (monocotyledons). All orchids have the same bilaterally symmetrical flower structure, with three sepals, but the flowers vary greatly in color and shape.
Questions related to Orchidaceae
I collected this Dactylorhiza from NW of Iran in a wetland at 2500 m. I found only one individual between many individual of Dactylorhiza umbrosa. As I know, this kind of Dactylorhiza with such white flower have not been observed so far in Iran. I don't know it is a diffrent species, a hybrid or just a variation in color of flowers within Dactylorhiza umbrosa? please help me to identify that.
thanks so much
I am writing an article for my blog regarding orchid viruses aimed at home gardeners. It will include details of home testing kits their use and accuracy. I also want to include details about whether you have to destroy a plant with a virus. Would anyone like to do an interview with me please? You can see my website here: www.littleflower.business
I am afraid that I will kill the seeds by crutial concentration of EtOH and NACIO. May I get some good protocol or information?
Hello,
can you tell me I which coordinate system are locations for P. grandis. I tryed HTRS96 which is Croatian official coordinate system, but is not right.
New localities of rare NATURA 2000 species: Pulsatilla grandis Wender.,
Genista holopetala (Koch) Bald. and Cypripedium calceolus L. in the NW
Dinarides in Croatia
Thank you.
regards,
Matej Ivencnik
For identification of epiphytes in Cloud-/Dry-forest in Ecuador (Loja Region).
Regarding vascular plants: Orchidaceae, Bromeliaceae, Araceae
I'm used standard squash technique using fuelgen staining.
I am planning to start working with mycorrhizae in orchids. There are many methods. I would like you to share your experience on the topic.
Goodyera repens (L.) R. Br. is a slender creeping orchid that inhabits coniferous or, more rarely, broad-leaved woods in the shade or semi-shade of the undergrowth. It presents a wide area of distribution along temperate and boreal regions of the northern hemisphere. Although some orchid guides refer its blooming period as June to September, more detailed works dealing with specific territories indicate that the blooming times occur mainly between July and August (Flora iberica: “VII-VIII”, Flora of the U.S.S.R.: “July-August”, Flora of China: “Jul-Aug”, Flora of North America: “early Jul–early Sep”).
In 1991 we found in the Maestrazgo mountains (Eastern Iberian Range) some populations of this orchid that represented at that time the first records in the Iberian peninsula outside the Pyrenees, extending its southern limit in the peninsula by more than 200 km (FABREGAT & LÓPEZ-UDIAS 1993). Then, populations were in bloom from mid-July to mid-August. In later years, new populations have been found in this environment, and recently (SERRA et al. 2016, FABREGAT et al. 2017) new locations have been encountered in the southern limit of this Maestrazgo area. In those locations, the plants nowadays begin to bloom in mid to late June. After 25 years, this orchid seems to have advanced about a month its blooming time.
Before starting a monitoring protocol to objectively evaluate this process in our region, I would like to know if this phenomenon has been observed in some other territories, especially in the southern edge of its global distribution area.
Ok so this is an odd story but I have 2 tubes of Cypripedium acaule seeds contaminated with a bacteria that's forming a whitish film over the T839. Surprisingly, the seeds are germinating more quickly, but I should replate soon. I don't suppose T839 is the best medium if I want a good symbiotic relationship. Should I look into a sophisticated, starch heavy medium (orchid seeds can't take in starch but they like the digestion byproducts from it)? PDA? Oats medium? Something else? Open to any suggestions
A lot of classifications exist in the Orchidaceae family in our days which make the publisher very confused in choosing the name of the orchid. Do you agree, and what do you suggest?
We have set various in situ experiments with epiphytic orchids seeds. We put fresh orchid seeds inside nylon mesh packets (ca. 1000 seeds per packet) along with a bit of moss (to improve moisture), and then located those packets on tree branches close to mother plants. After 1 year, we retrieved the packets and open them to locate germinating seeds, but moss and lichens have grown inside of the packets, plus there is a large accumulation of detritus and dirt, so it has been very difficult to locate the seeds (only finding <5%). We don't expect mortality/decomposition rates to eliminate 95% of seeds.
Do you have a recommendation on how to locate those seeds?
We have tried the following:
1) series of washes and filters to remove bigger pieces of moss and lichens
2) washes and low centrifugation
3) centrifugation with filters
4) dilution of centrifuged materiales in several petri dishes.
We wish to use a method that wont damage the putative fungi growing in the germinating seeds / protocorms.
Thank you!
It's a small saprophytic grass (China), I am sure it not belongs to Orchidaceae. Does anyone know?
I am making a research about the bee foraging plants.
It is known that pollen contents of honey that comes from nectariferous plants reflects the botanical origin of honey.
But some plants such as orchid and Anacamtis (orchidaceae) produce pollinium rather than the pollen grains that famous to all people.
In my field observation i saw bees visit Orchidaceae plants & feeding on their nectar. My question is there any article or publication or a way describes how to detect honey originating from Orchidaceae which produce pollinia rather than pollen or it is not possible.
My second question Rex Sawyer in his book (honey Identification 1988) showed pollen grains of Asclepias Sp. in honey from North America. What is the intertpretation of this because asclepias produces pollinium not pollen (see its picture in https://www.discoverlife.org/mp/20p?see=I_JLL38&res=640). Is it because the pollinium rapture and releases its pollen which detected in the honey.
Martins, D. J. & S. D. Johnson. 2007. Hawkmoth pollination of aerangoid orchids in Kenya, with special reference to nectar sugar concentration gradients in the floral spurs. American Journal of Botany 94: 650–659.
Nilsson LA, L. Jonsson, L. Reason & E. Randrianjohany. 1985. Monophily and pollination mechanisms in Angraecum arachnites Schltr. (Orchidaceae) in a guild of long-tongued hawk-moths (Sphingidae) in Madagascar. Biological Journal of the Linnean Society 26: 1–19.
Peter Roos
Key identification of Orchidaceae family
The culture is 4 months old and I have used activated charcoal (1%) and citrate (1%) to avoid browning of the PKB's and Seedlings.
I am trying to extract RNA from the pseudo bulb part of a medicinal terrestrial orchid. although I got high quality RNA from leaf but while trying so from the pseudoibulb or rhizome I am facing huge issues. I have tried qiagen extraction kit and other modified CTAB based extraction modules. However I failed to extract good quality RNA for NGS. It will be of great help if you can please suggest any effective protocol which can eliminate this shortfall.
I want to cultivate the orchids in field conditions. Do you have any research on this?
Hello colleagues! im actually revising some Peruvian Stelis (Orchidaceae) and I encounter with a confusing issue. Maybe someone here could help me. On his latest work, Luer described S. miranda a newly species from Colombia. His notes mention its resemble with three Bolivian species: S. antennata, S. saltatrix and S. ballatrix. Here comes the problem! I was unable to find any reference to the last two species mentioned. Apparently S. saltatrix or S. ballatrix non-exist either on Tropicos or IPNI. Does anyone know where this two species were published?
Thanks!!!
Recently I took a photo of this orchid (Sobralia) in the northern Peruvian Andes (2000 metres above sea level) Can anyone identify the Species ?
Thank you very much !
Stefan
Recently I took a photo of this orchid in the northern Peruvian Andes (2000 metres above sea level). I think it is maybe a Phragmipedium boissierianum, but I am really not sure. Could you please help me – thank you very much.
Greatings from Chachapoyas, Peru.
Stefan
i need to know the maturity of my plant for my undergrad theisi
Identification of species and cultivars from Doritaenopsis and Phalaenopsis
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Hi guys,
im looking for some photos of Palmorchis lobulata (Orchidaceae) to include on an article which im working, any help?
Thanks a lot!!
I proposed a study with regards to the said plant. I will be using the pseudobulbs for my study. How many days will I be able to have a fully-matured pseudobulbs and what are the good fertilizers that can be used to fasten the growth of the said orchid.
I'm currently revising a Stelis (Pleurothallidinae: Orchidaceae), and i noticed that represents a new species. However, the area where i took the photo have been deforested (....). I´ve tried to find it on nearby locations without any success. So, any suggestions?
The Orchidaceae family in Iran is represented by 46 species and subspecies.
For conservation, I want do the tissue culture of some species.
I have collected an epiphytic orchid. Photo of this species in flowering and fruiting condition is attached. I need help from Taxonomists, Scientists and Research scholars for correct identification of this orchid.
I have added some more photos as suggested by different viewers for better identification.
Description of photos
3) Mature Flower
4) Column with anther under simple microscope 10X
5) Column with anther about 2.5 mm
6) Young flower under simple microscope 10x
7) Spur with column
8) Pollinia
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Hello everyone, I read the following article (https://www.researchgate.net/publication/227230335_Random_amplified_polymorphic_DNA_analysis_of_the_moth_orchids_Phalaenopsis_Epidendroideae_Orchidaceae) and I the teacher's group told me to find a way to isolate Orchids DNA but on our lab We don't have the founds to buy/import the DNAzol, I want (if Its possilbe) to get the composition of the reagent for talking with the laboratory workers If We can build up a similar reagent with the specifications, thanks everyone.
Ophrys omegaifera subsp. apollonae
We have observed that Iris flower (each of three meranthia) is only once visited by bees for nectar foraging, the other visits are prohibited by negative visual or smell attraction of the nectarless flower. Is this possible? I suppose it may occur in Orchidaceae or Asclepiadaceae families. What is a name of such type of flowers?
Does anybody know whether such a classification of flowers exists or not, when flowers are classified after number of the pollinator visits – one, two or many that are necessary for both male and female functions may be realized. It depends on nectar production during male and female phases (if dichogamy takes place). It also may depend on the number of stigmas in the flower and longivety of anthesis.
Can any help me in finding type species for a orchid genus Crepidium.
Botanists specializing in Orchidaceae often use the Kew mixture to keep original size and shape of orchid flowers. I was wondering if this mixture allow to keep natural shape and structure of orchid pollinia too. In other words, if I decide to preserve orchid flower in the Kew mixture and later decide to take some pictures of pollinia using SEM microscope, may I expect that they will look like from fresh flowers? Or maybe their structures will be changed (distroyed)?
Thank you in advance for your comments.
Best regards,
Radomir
I'm currently working on this genus, i appreciate any help,
thanks!
I am looking for published data about lifespan of miniature orchids species living in natural habitats (mainly belonging to these three genera mentioned above). Are there any studies upon such topic? Is the lifespan of such orchid species living in the wild much longer that in laboratory/home culture? Is there any correlation between orchid size and its lifespan?
Thank you in advance for any comments and suggestions.
Best regards,
Radomir
Hello,
I would be very grateful for any morphological details allowing to recognize two Orchidaceae species - Restrepia lansbergii and R. dodsonii (first is known from Venezuela, Peru and Ecuador, the second is endemic to Ecuador).
Maybe there is any key for identification plants belonging to this genus?
Thank you in advance for your help.
Best regards,
Radomir
I have got Coelogyne pods and Knudson C medium for orchid seed germination, but since the seeds are too small and fibrous to handle, feel it extrmely difficult to do and sow uniformly on the medium...
shall i prepare the medum on petriplates or jam bottles....
sufficient literature is available but I feel practical difficulty........please help......videos can be provided........for convenience............
I have seen papers on DNA bar coding, where some are clamming species approach is more acceptable, again some are claiming genus specific approach is more acceptable. I want have a view which will be more acceptable?
Where can I download the references related to the ethnobotany (local use, traditional medicinal use etc.) of Dendrobium helix?
Many studies indicate that the germination of orchids needs assistance from mycorrhiza. However, in-vitro germination is very common for orchids (especially epiphytic orchids) cultivation. I wonder whether orchids need a fungal partner to germinate or to grow soon after germination?
We have no idea what these are--but they are present in growing cells behind meristems and appear to break down as the cells mature since they cannot be found in older cells. Any information on what this structure is will be most appreciated.
I had germinated the orchid seeds and got plantlets. Then I have used the raised plantlets as explant source. I have found that the plantlets which were derived from these explants as source are having high genetic stability or otherwise low rate of variation.Noteworthy to mention, the wild populations are having a lower degree of gene flow. What can be the possible reason of this outcome? Secondly, as we know seeds are generally thought to have a greater degree of diversity. How can we justify this with our finding?
Generally, its thought that seed raised plants of a crosspolinated species have high genetic variability. To crosscheck it , I carried out an experiment where I found lesser degree of variability, ranging from 2-8%. I extracted DNA from hardened and acclamatised plantlets of a crosspolinated orchid species and carried out its stability analysis using RAPD and ISSR markers- I found that the percentage ranged from 2% to 5%. What can be the possible reason? Does PGR have a direct implication on its fidelity? I am attaching a paper which i followed while doing this analysis.
Which wavelength will produce responses in plant tissue culture?
I want to establish cultures of certain dendrobes from ex vivo source.Can you please suggest the PGRs whose combinations/individuals are ideal?
Angiosperms are defined by double fertilization, one sperm cell from the pollen tube fertilizes the egg cell and the other one forms the endosperm. In orchids there is no endosperm. So is there double fertilization in orchids?
I am searching for an epiphtic orchid named Dendrobium corymbosa.But I am not getting any deatails about I came to know from a researcher that the species name has been changed.Can anybody provide me the details of this plant?
New proposals suggest changes in the generic classification of orchids, but are those real entities or just a way to develop the ego of orchid taxonomists?
Orchidaceae is a charismatic group of plants and everybody would like to know how many species are there.