Questions related to Ophthalmology
I am currently attempting dissection of the mouse aorta to expose the endothelium for Oil Red O and other standard stains. I am using ophthalmological scissors to open the vessel from the outer curvature and I pin the vessel along the inner curvature to secure it.
However, I find myself slipping out and 'getting lost' in the vessel, and I cut several times as I try to find my way back in. Sometimes it seems ambiguous whether I actually entered the vessel or not. As a result, my final tissue preparations have edges that are not clean and defined. I am also struggling with pinning straight, with some orientations of the prepared aorta seeming ambiguous.
I am looking for any technical tips on how to make my cuts more defined and clean so that the final endothelial presentation looks good enough for a publishable figure. Does anyone have any advice on this?
I attach pictures of vessels I have attempted, as well as a link to a quality of preparation in a figure style that I would ideally like to replicate!
Thank you so much for looking this over and for helping me out.
I have ophthalmology resident trainees who want to perform a systematic review and/or meta-analysis in glaucoma and want opinion from other experts about what topic seems relevant for them in this specific field
Currently, the powder form of pilocarpine nitrate is not available in chemical companies in my country..So can i use commercially available pilocarpine nitrate eye drops for intraperitoneal injection in rats (used once to stimulate salivary flow at the time of sacrifice) ?
Thanks in advance.
The literature is conflicting with approximately half of the studies reporting no change in colour vision and the remainder a change. The interesting group of patients are those with a tinted implant in one eye and a plain one in the fellow eye. There is a case report of one such patient demanding the tinted implant to be explanted
I have performed a survey of ophthalmologists' attitudes towards vaccinations for patients with corneal transplants and the controversial and weak association with rejection. In trying to write up the paper, I am struck at the lack of evidence for these concerns in the ophthalmology literature (4 historic case series/papers, and 2 contradicting larger studies). Can any ophthalmologists, immunologists or solid organ transplant teams comment from their knowledge of their literature within their field? Is there an agreed scientific basis and mechanism for these concerns (even if theoretical)?
A young female patient was diagnosed to have cone dystrophy and she wants to know if there is any cure including gene therapy or even anything that can stop progression
I am a graduate student in ophthalmology and I am currently hoping to write a review or meta-analysis in diabetic retinopathy or high myopia, and I would like to ask research experts in the related field what they would recommend.
The behavior of bacterial/fungal keratitis do not not allow the doctor to wait for laboratory support to start treatment as every moment is crucial in saving the cornea. After having sent the swabs for identification of infecting organism and culture/sensitivity reports, the best option seems in following "Imtiaz's law of inversely proportional hypopyon" which states that in fungal keratitis, hypopyon is less than the size of ulcer in contrast to bacterial ulcer where hypopyon is usually more in size than the ulcer area relatively. Therefore immediately embarking on treatment on the basis of this law seems justified.
Ref: Shah SIA et al: Concise Ophthalmology Text & Atals. 5th ed. Param B (Pvt.) Ltd. 2018: 48-49
Delhi, Tamil Nadu, and Kerala Ophthalmological societies have their own journals which are unfortunately not indexed in pubmed? What's your take on publishing works in these journals as first choice?
This occasionally occurs following IntraLase but how do colleagues manage the patient and is the outcome affected ?
I'm part of a team designing an observational study in which we're going to compare 2 models of intraocular lenses. The main outcome is contrast sensitivity.
I'm having some difficulty in understanding how we are going to handle this variable in statistical analysis and looking at the literature hasn't helped me much. It's also important because, being the main outcome, our sample size calculation depends on that.
Has anyone worked with something similar and can give me some pointers?
With the ever increasing potential of using Big Data in Healthcare in India, this discussion is aimed at sharing your experiences and challenges in publishing from large clinical datasets in Medicine.
As a researcher, you may be frustrated in finding continuous funding for sustaining your research. However, would there be any conflict of interest to be a researcher as well as a fund raiser simultaneously at the same time?
What regulations are there to limit the grey area?
Which authorities were looking on such issue?
What if the fund raising is for charity organizations in the field (particularly pediatrics and rare diseases) and is unrelated to the researcher's work?
In medicine field, the practice of physicians fundraising from their own patients raises three main concerns:
(1) undue pressure on patients to contribute,
(2) possible expectations of preferential treatment from donors, and
(3) concerns about patient confidentiality and trust.
What do you think?
In an ophthalmology research involving endothelial examination by specular microscopy, there is a lack of correlation between the endothelial cell count and cell density, as is shown in the figure.
The results of specular microscopy examination on the 3rd month post-op shows that the mean endothelial cell count of the "red" group is lower than the "black" group, while at the same time the cell density of the "red" group is higher than the black group.
I expect that the inherent correlation between "count" and "density" would mean that one group would rank higher or lower regarding both variables; but I'm thinking that I might be wrong.
I am not an expert in specular microscopy, so I would appreciate it if someone could tell me if the data presented in this study can't be right and is impossible or is there a scientific explanation for this lack of correlation?
I am a medical intern, I am asking the experts to suggest topics in ophthalmology ( cross-sectional ) that can be conducted through an online questionnaire in a specific region, for the general population.
With increasing COVID-19 infected cases worldwide, people around the world are practicing strict personal hygiene against infection. Alcohol-based hand sanitizer was one of the best sellers, but will it cause eye injury?
What if it is accidentally split into the eyes?
Will prolonged exposure to the evaporated alcohol cause eye problems?
Have you even encountered such an injury in your life or medical practice?
Details about good Ophthalmology journals publishing good work without the financial burden for a young resident in a peripheral centre.
I'm seeking researchers (basic and clinical ) with interest/experience in eye /ophthalmology to submit articles for a special issue. The issue will be in a major, international, Swiss based and PUBMED listed journal). Tentative title : Stem Cell Research in Eye and Ophthalmology- Current Advances and Future Directions.
Any authors considering publication of their work shortly, please contact me IMMEDIATELY to express your interest.
Steven Levy MD
Study Director: Stem Cell Ophthalmology Treatment Study 1 and 2.
1. Can academic trial be conducted on platelet rich plasma in india? 2. Is there aby permission required from specific do conduct research on prp? 3. Are there spring guidelines on who and where prp can be prepared? For example if a dermatologist want to do research on prp, can he prepare it in his department? 4. who can prepare prp? is a MD student from dermatology or Opthalmology or any specialty can learn how to prp preparation from transfusion medicine and prepare it? Or it can be prepared only by a professional from transfusion medicine? 5. What are the approved indications for use of prp? 6. Are there any specific guidelines for method of preparation of prp? 7. Are they any specifications for the site where prp can be prepared? I will be highly thankful if the aforementioned queries are addressed by you.
Do you know the name of such referencing style? Any software suggestions for that? I have been asked to do that when submitting manuscript to survey of ophthalmology. This is Weird
I would like the article:
Bilateral idiopathic cystoid macular edema: Report of four cases---Tabatatbaee SA, Soleimani M, Rajabi MT, Kiarudi MY.
International Journal of Ophthalmology 8(11): 2182-2184 November 2008
Can you please send it to firstname.lastname@example.org
There are very few case reports of ATONIC PUPIL after uneventful cataract surgery and that too in old literature.
With ATONIC Pupil I mean, FIXED DILATED (~8mm or more) PUPIL without any damage to iris structure.
In your journey in Ophthalmology, If you have faced any such situation, please share your experience and how did you manage that case.?
I am trying to understand how the depth information in SD-OCT A-scans is obtained over distances far in excess of the source coherence length.
From what I have gathered so far, it seems that the fringe frequencies within the spectrally resolved interferograms encode depth information, and that this frequency is proportional to path length difference. What I really don't understand is why, and how this information is so precise as to be allocated a specific pixel depth.
Underlying this question is also that of the importance of source bandwidth. I *think* understand the notion of coherence envelopes, and it makes sense to me that path differences corresponding to integer multiplications of the coherence length give rise to new intensity minima and maxima, ergo the axial resolution of the system. But I had considered coherence length to be an intrinsic property of the source, and yet, the spectrum must be spatially (SD-OCT) or temporally (SS-OCT) resolved into narrowband components before Fourier analysis. Am I right to assume then, that wavelength dependant information is aggregated either before or after the FFT? If so, or even if not, how does backscattered light interfere more frequently with the reference arm? (I believe that I have been misled by the false premise that light will interfere if and only if its is superposed with its identical twin "packet", where it diverged at the coupler?)
I have inspected the Fourier transform equations of intensity as a function of spectral band width and indeed, time delay (correlating to depth) is encoded. What I fail to understand is not the maths, per se, but the intuitive behaviour of the light that gives rise to these equations. A lot of the literature evaluates the maths but so far, I do not have an intuitive, or qualitative understanding of the physical waveforms interfering with the reference beam from different depths and how this translates to predictable variations in fringe pattern.
This short paper: Measurement of optical distances by optical spectrum modulation from A. F. Fercher et al. , in part describes more elegantly my clumbsy question above, but proceeds to answer the question (page two onwards) with maths that I haven't been able to interpret.
My background is biomedical, so perhaps the notation and syntax of the predominantly mathematical descriptions are a barrier to this.
Frustratingly, TD-OCT is relatively a lot simpler, and I've been able to decipher that without much ado.
Thank you in advance - I apologise for the lengthy question.
The neurologic examination is a powerful tool for urgent bedside assessment of ICU patients with neurologic or neurosurgical illnesses. Assessing cranial nerve function is one of the most vital components In this context. Testing the pupillary light reflex evaluates the status of the second and third cranial nerves. Automated pupillometers have been developed that provide objective measures of size of the pupil and the responsiveness of the pupil to light (neuropupillary index). Although few studies reveal diagnostic and prognostic usefulness in critically ill patients, none of the studies correct/adjust for interfer and confounding effects by for example sympathomimetic or parasympathomimetic drug effects, interference from ambient light, previous cataract operation, etc.
Isn‘t it much too early to reliably use automated pupillometry for diagnosis and prognosis in daily clinical practice in ICUs?
Vernal keratoconjunctivitis (VKC) is erroneously called as spring catarrh as it occurs in hot months and the better term should be a summer catarrh.
Ref: Shah SIA et al: Concise Ophthalmology Text & Atals. 5th ed. Param B (Pvt.) Ltd. 2018: 36-37
Which device would you use as a gold standard for verification of the project relate real keratometry of the eye. I want to verify the anterior, posterior and the real power, Sim K of the posterior, anterior and true values.
I was thinking about Casia 2000 SS-OCT from Tomey but they do not have FDA. So, what would be the best reference for the prototype software: maybe Pentacam or GALILEI from Ziemer.
In simple and convenient terms, which terpenes improve vision? Are there terpenes, when inhaled, that can affect visual perception? Can they improve our ability to detect or process illusions? Can terpenes change our perception of color intensity? Do terepenes affect any enzymes associated with the functions of the eye?
The most interesting thing found so far was in a history book; Van Gogh also had some digestive problems, for which he may have taken santonin, a terpene, used at the time to treat and prevent intestinal parasites and known to cause yellow vision. This may have impacted his work.
It has been difficult to find much research related to terpenes and ophthalmology, eye health, or visual perception. I am interested particularly in terpenes found in cannabis and that are also abundant in nature.
I would appreciate any information about various terpenes such as pinene, linalool, myrcene, beta-caryophyllene, etc.
I have found some essential oil safety data, that some increase the number of eye blinks because they are irritants. additionally, any data about CBD and its effects on IOP would be appreciated.
Recurrent chalazia are seen to harbor adenocarcinoma clinically and on histopathology examination 1.
1. Ref: Shah SIA et al: Concise Ophthalmology Text & Atals. 5th ed. Param B (Pvt.) Ltd. 2018: 27-31
Retinal Specialists keep on focusing their attention on Rhegmatogenous, tractional and exudative retinal detachments and never even like to listen about solid retinal detachment so much so that one of my colleague who is a wonderful retinal specialist was almost annoyed to see inclusion of solid retinal detachment among various types of retinal detachments in my book1.
1. Ref: Shah SIA et al: Concise Ophthalmology Text & Atals. 5th ed. Param B (Pvt.) Ltd. 2018: 85-95
Result of Investigations
Research Oriented Aspects
Ref: Shah SIA et al: Concise Ophthalmology Text & Atals. 5th ed. Param B (Pvt.) Ltd. 2018: 11.
I know the OCT Spectralis is having Blue Peak, Multicolor, autofluorescence and you can add ICG and Fluorescein AGF to the platform.
With the OPtovue, you do SD-OCT and OCT-A. that is all.
Comparing just the SD-OCT and OCT-A between these devices, which one is better for you,
Thank you in advance.
Genetics seems to have generated immense knowledge about various eye diseases. These findings include gene mutations, their effects, pattern of inheritancel etc. So, Is this the time to have ophthalmic genetics units in every ophthalmology set up? If yes, what would be its profile and how would it support the set up?
Minimum rim width has been adapted by recent OCT devices since it takes more into account variability of the entry of the RNFL axons into the optic nerve canal
Should ophthalmologists consider treatment options beyond intraocular pressure ? What will these options be? There is normotensive glaucoma also; what should the approach be in those cases?
IT is a picture of the fundus. I am not sure about what is this indicative of and what landmarks should I take into consideration. I have acquired a lot of such images but I am not able to interpret them.
I am formulating a Ophthalmic eye drop solution and I want to claim that the eye drop contains a "Disappearing Preservative" that becomes water and Oxygen once in contact with the eye and the light, and that EDTA is used as a Chelating Agent. But if I use the EDTA at 0,1% concentration, then the solution is no longer free of preservative once instilled in the eye. What justification can I give for the use of EDTA, so that the use of this agent is not viwed as a preservative?
I have some doubts about the short-term mechanisms of action of Rho kinase inhibitors on corneal endothelial cells. Can someone help me? I am not finding the information I need in the literature.
As you may know, Y-27632 has been extensively evaluated in pre-clinical studies aimed at the treatment of corneal endothelial lesions. The inhibition of the ROCK signaling pathway with Y-27632 results in inhibition of myosin light chain (MLC) phosphorylation, with subsequent inhibition of apoptosis of corneal endothelial cells. The literature shows that 100 hours after treatment with Y-27632, endothelial cell cultures show totally collapsed stress fibers and a significant reduction in the apoptotic index. However, I wonder what happens within the first 15-30 minutes after exposure of the cell to the Rho kinase inhibitor? Is any significant effect expected on cell metabolism or activities in such a short period of time? Finally, considering the rational clinical use of Y-27632 and possible ocular adverse effects (ocular hypotension, intense hyperemia, etc.) of its instillation, is inhibition of Rho/ROCK advantageous in which situations that affect the corneal endothelium? Would you use Y-27632 only to prevent cell loss in a healthy corneal endothelium?
I want to use it in order to explore dry eye symptom (Foreign body sensation,Itchy eye,Burning sensation,Teary eye,Photophobia,Red eye) reporting patterns and to better understand symptomatic heterogeneity in the study population.
how many group should I do and according to what the subjects can be put in groups ?
Note : each dry eye symptom was grade as 0 (none of the times ) , 1 ( some of the times) , 2 ( half of the times ) , 3 ( most of the times ) , 4 ( all of the times )
The new IOL master 700 has smaller in vivo repeatability SD for most metrics than the Lenstar, eg. 8µ instead of 35µ for AXL, 11µ instead of 40µ for ACD and 12µ instead of 80µ for lensthickness.
However, are these smaller standard deviations of clinical importance, i.e. to what extend would they contribute to a different choice of IOL power in in 'old' formulas like e.g. SRK-T and Holladay 1, and in new formulas, like e.g. Olsen or Barrett ?
Retinal surgery utilises the viewing system of operating microscope and various additional lenses. Visualisation becomes a challenge If the viewing lens becomes fogged.It is mainly due to water vapours present in operating room and patient's breath. How can this be eliminated so that surgery can proceed uninterrupted?
A comparative study with various post operative inflammatory parameters has shown
As in the subject - do you know any comercial systems for computer-based campimetry/perimetry, where light stimuli are displayed on the flat computer screen? I know only common perimeters based on the hemispherical surface stimuli presentation, such as Humphrey Field Analyser or Medmont M700. I'm also familiar with the software for psychophysics listed at http://www.hans.strasburger.de/psy_soft.html.
Retinally induced aniseikonia may be an increasingly important cause of visual symptoms with the aging of the population (Rutstein, 2012). The prevalence of RIAk is unknown because it is not routinely teste in the clinic. Are there any differences by 2012?
As,Tracheal bleed during intubation from tracheal erosion,bronchial spasm,apnea etc,high INR,superadded infection,compromised immunity,etc etc.... makes decision making little troublesome in such cases, especially to me often. If anybody has any experience ?please share.
As found in the literature The OSDI is a valid and reliable instrument for measuring dry eye disease it is assessed on a scale of 0 to 100, with higher scores representing greater disability. The overall OSDI score defined the ocular surface as normal (0-12points) or as having mild (13-22 points), moderate (23-32 points), or severe (33-100 points) disease.
I think the cutoff should be > 12 , is it correct ?
I currently work with the Ophthalmology department at the University of Illinois at Chicago. I have some ideas in mind which would require a Magnetic hyperthermia instrument.
I was wondering if anyone knows who has the equipment in the city so that I could contact them and look for a collaboration or solicit them to use the machine
We need a young and updated researcher in the field of eye research. We need his/her clinical knowledge to complete our discussion section in a manuscript pertinent to eye and ophthalmology. Please contact me for further information.
We all like brand new charts for our clinics and trials etc. but I have a number of old charts - either yellowed plastics or grubby looking Pelli Robson's etc. I'm struggling to find anything on the published shelf-life of these products. Can anyone assist?
Flomax causes Intra-operative floppy iris during cataract surgery. Does it effect zonules in any way?
In humans it stays upto 24hrs to 72hrs and I'm expecting it to be longer in rats. I'm using a 2% homatropine hydrobromide solution for an experiment on rats and i want to know the dosing interval for continuous mydriasis. I need it for 9 days straight but sadly atropine eye drops, here, are not in production any more.
Is it safe to assume that it acts longer or more potent? Any precautions to keep in mind?
Does your hospital has any guideline for preoperative PHARMACOLOGY management of cataract or glaucoma surgery?
I am trying to collect as many opinions from all over the world
What could cause autologous serum eye drops to precipitate? A patient has been using the drops for years due to dry eye symtpoms. Recently, three of four production series tended to precipitate when being thawed. Cold agglutinines, cryoglobulines, bacterial contamination was ruled out by lab examinations. FVIII (as one of the factors in cryoprecipitate) was only slightly elevated to 180% (upper norm limit is 150% at our lab). Electrophoresis of serum proteins was normal...any suggestions?
I am trying to find any information about number of performed vitrectomies world wide as well cataract surgeries?
Do we have everyday increasing number of vitrectomies? Is there any data or any article to compare these important two aspects?
After placement of an orbital implant in enucleation, the anterior layers (ant Tenon's and conj) are simply closed. <br />
In the interest of increasing (eventual) ocular prosthesis motility, would it be advantageous to tack these layers together?<br />
Perhaps with an alcohol to denature some fat, followed by a mild caustic, suctioned, then irrigated-out?
Globally, the magnitude of occurrences of Pterygium is over 200 million people and the prevalence varies between 0.3 % and 37%.[i] The fact remains that ocular disorders are caused by the reflected solar radiation from the surface of the earth especially the white and shining surfaces such as snow and water. [ii]
My question is what percentage of Out Patient cases in hospital undergo surgeries?
[i] Lu P, Chen XM., Prevalence and risk factors of pterygium. Int J Ophthalmol 2009;2(1):82-85
[ii] Bergmanson JP, Soderberg PG. The Significance for Ultraviolet Radiation for Eye Diseases. A review with comments on the efficacy of UV Blocking Contact Lenses. Ophtahlmic Physiol.Opt. 1995; 15: 83-91.