Science topics: LamialesOleaceaeOlea
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Olea - Science topic

A plant genus of the family OLEACEAE. The olive fruit is the source of olive oil.
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how can i do lyophilisation of psv pseudomonas savatanoi islated from olive knot ?
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Stephan D, Da Silva AP, Bisutti IL. Optimization of a freeze-drying process for the biocontrol agent Pseudomonas spp. and its influence on viability, storability and efficacy. Biological control. 2016 Mar 1;94:74-81.
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I have started research work on the above said topic. Need literature related to my topic for literature review.
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There are several publications that you can find throught Google, specially in producers countries, then you may have a complete papers fron Spain, Italy, Turked and some others.
In my country we do not produce olives.
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Dear researchers,
I am going to use CROPWAT 8.0 in order to study olive crop. The study in the north of Iraq where the latitude is 36.31 N and longitude is 43.15 E. In the crop module of the software, there are some values that are ready to be taken from the software. Unfortunately, these values are not available for olive. Can anyone help me to get these values?
Thanks
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السلام عليكم دكتور
ممكن اعرف Ky للمحصول الزيتون لان اني طالبة ماجستير في جامعة البورصا التقنية واحتاج احسب احتياج المائي
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I'm doing a research about pruning intensity and the impact on a olive tree pests and natural enemies. I couldn't find that much literature.
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Dear Elhoucine,
I am sharing a nice manuscript on pruning impacts on olive tree architecture and annual canopy growth.
Best Regards,
Jean
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The olive tree is resistant to negative temperatures but not above -10°C, and this only when it is already well established and the soil is well drained. I would like to know if there are any olive varieties in other parts of the world, other than the Mediterranean region where it is cultivated and of productive interest.
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There are hundreds of cultivars of olive. Olive trees can be grown in southern England and on sheltered and coastal sites elsewhere in the UK. Mature trees are winter-hardy but dislike protracted winter cold. A small area of olives is grown commercially or semi-commercially in England. A warm summer in full sun is required for a good crop. Tuscan and French cultivars are said to be relatively hardy, including ‘Frantoio’, ‘Leccino’, ‘Cailletier’ and ‘Maurino’ (a good pollinator for ‘Leccino’). ‘Leccino’ is also said to be resistant to Xylella fastidiosa.
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When we measure the color of small fruits (ex. olive, grape, cherry) by konica minolta CR-410 (large head diameter) using white background, are the results correct? If not, how can we solve this issue?
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Is any have experience on DNA extraction of Olive silica dried leaves through kit method or any other method?
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Dear Junaid, thank you for asking this interesting technical question. A reliable DNA extraction method from olives has been described in the following research article entitled:
DNA extraction from olive oil and its use in the identification of the production cultivar
Also please have a look at the following potentially useful reference:
SILEX: a fast and inexpensive high-quality DNA extraction method suitable for multiple sequencing platforms and recalcitrant plant species
Both articles are freely available as public full texts on RG.
Please note that there is also a closely related previous question on RG:
Is there anyone who isolated DNA from the veins of the olive leaf?
Unfortunately this question has received only three answers so that is perhaps only of limited use.
Good luck with your research!
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Hello all, I need your help,
Listen, I have a case study with an olive mill and the problem is how I can use capacity in one process that has 2 machines with capacity 1500 Kg olive fruit per hour each machine. So the capacity in Resources for this Process is 2, because i have 2 machines and Units to Seize/Release are 1500?
Is this wrong or not? Please help me if you can
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Of the varieties of olive trees cultivated, it would be interesting to know which are richer in antioxidants in order to plan future plantations, where it is important to relate health to olive oil consumption.
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Dear @J. Antonio Cortiñas Rodríguez. PhD.
Olives are particularly rich in antioxidants, including oleuropein, hydroxytyrosol, tyrosol, oleanolic acid, and quercetin.
I have attached four PDFs; hope these will be highly useful to you.
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Olive tree leaves start wilting, leaf rolling
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For a techno-economic analysis of pyrolysis of olive mill waste water sludge, I am looking for the price of fluidized bed reactor. The feed rate is 4000 kg/hr.
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Dear @Muhammad Shoaib Ahmed Khan
Oir experience ist 1000€/kW - 3000 €/kW dir installed Power by combustion. For pyrolysis ans gasification you have to Double the Price.
But there are Big differences in place, Materials you want to use and so on...
Feel free to contact us
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Especially, we refer to the presence of molecular phenol in OMWW as a general problem. "The fraction obtained at the end of the batch membrane operation appeared to be rich in polyphenols and should be processed for their recovery in regards to the negative impacts of phenol".
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Dear all,  
I am currently a guest editor for the special issue "Mediterranean Olive Trees and Olive Oil under Climate Change", which will be published by the journal Agronomy (ISSN 2073-4395, 2019 JCR impact factor = 2.259, Quartile 1).
The deadline is June 2021. Please feel free to contact me if you are interested and also feel free to disseminate this message in your groups.
Yours sincerely,
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Bon courage et bonne chance Olfa
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We're looking to purchase olive pollen for an artificial pollination study. Does anyone know where we might be able to buy Olea europaea pollen?
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Most commerical Olive cultivars are self unfruitful so that it needs cross-pollinated through either cultivation more than one cultivar in an orchard or hand pollinated (not advised), and for experimental purposes, you can use pollen from compatible cultivars.
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If want to isolate bacteria from olive knot from diferentes area and we want to have a good collection of bacteria for molecular identification can we íolate in any time in the year ỏ must to íolate ony in spring? Thank you
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I'm looking for the real numerical data of the Xylella fastidiosa epidemic in Olive trees to do the mathematical modeling of this disease via fractional derivatives. If anybody has then please share with me.
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Dear Pushpendra.
please see attach file.
Best Regard
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Aristotle is rumoured to have said that; "It is easy for philosophers to be rich if they choose, but this is not what they care about." He apparently said this after seeing Thales of Miletus turn his fortunes around after living in poverty and being taunted with the uselessness of philosophy."
He is reported have made a good fortune by entering into one of the earliest option transactions on "olive presses" in Miletus and Chios, leveraging on his ability to forecast a good harvest.
The question is do you agree that philosophers and academics can be rich if they choose to, but this is not what they care about.
NB: The story was sourced from: Desai, M (2017) The Wisdom of Finance: Discovering Humanity in the World of Risk and Return, London, Profile Books.
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Yes, they can be rich. For example if they were able to develop a product, patented, and commercialized, they will be receiving a royalty for life.
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I am working on a dataset to derive optimum and sufficiency ranges of leaf nutrient concentrations in olive. I am searching for a reliable reference to compare it with the ranges generated by me.
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Are there any Researcher working on infection with Defoliating pathotype of Verticillium dahliae on Olive Orchard.
I need information about these pathotype ( Country, Area, Environmental conditions, Rain rate,...etc).
Thanks to everyone read this question.
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There are many chemicals and PGR mentioned in the literature for rooting of Olive and I used IBA.
I want to know about the best one for better results from experts of Olive.
Please mention with concentration 😊
Thanks in anticipation
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I have used 3000ppm IBA in mine study
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In a 18th century private diary, surfaces are never measured thanks to units. Instead of that, the landlord registered each number of trees. That is why I should need to know what was approximatively the plantation density, even if it probably varied according to the slope and to the quality of soils and watering.
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Nice confirmation, thanks
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I wish to measure the activity of RuBisCO enzyme in olea without the involvement of radioactivity. Is there any method available
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If you are measuring the O2 consumption reaction to produce 2-phosphoglycolate and 3-phosphoglycerate, you could just use a Clark electrode and measure the depletion of oxygen. If you are measuring the CO2 consumption reaction, you could do a coupled reaction by adding excess 3-phosphoglycerate dehydrogenase and NAD+, which you monitor spectrophotometrically at 340nm. I don't know the optimal pH or any metals requirements for that enzyme off the top of my head, but I wouldn't be surprised if phosphate buffer was a poor choice, due to the probability of the phosphate being a competitive inhibitor with the 3-phosphoglycerate.
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olive leaves has a many active ingredients compounds like flavin ,tanine, and others which exact compound that play a critical role as a hypoglycemic effects in diabetics and what is the mechanism of action in such way ?
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It has to be validated either in mouse model or cell lines.
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In a Palestine Exploration Fund Quarterly Statement (PEFQS) article on the "History of Colonization in Modern Palestine" from 1900, the following was listed as one of the projects.
"1664 (circa.), Colbert, Minister of Louis XIV, founds a farm at Beit-Dejan, on the Plains of Sharon, near Jaffa. An grove of fine old olive trees is still pointed out as having been planted by him."
I'm looking for further information, sources, and documentation to understand this grove's location and chronology.
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Dear Giuseppe,
Thank you very much for your response. I had previously checked Gallica but didn't find any relevant results. I appreciate your pointing out that not all the scanned documents in this collection are OCR. Fortunately, some of the documents are searchable through other sources. The book you suggested and additional volumes are found on google books and are searchable. Unfortunately, I still haven't found any sources connecting Jean-Baptise Colbert to Jaffa. Best regards, Joseph
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Hi everyone,
As part of my PhD program I would require some Verticillium dahliae for inoculations on olive plantlets, preferably VD pathotype D of known virulence.
Is there anyone working with this pathogen willing to share their isolates or perhaps to share an information on where to obtain them?
Would appreciate any information you can provide.
Thank you.
Regards,
Kristina
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You can obtain V. dahliae strains from ATCC from which some are pathogenic. If not possible and you have some isolates of this fungal plant pathogen you can molecularly characterize with help of few primers which show band at 334 and 462 bp (specific for Defoliating (D) type).
You can take help from the following article:
Jimenez-Diaz., et al (2011). Region-wide analysis of genetic diversity in Verticillium dahliae populations infecting olive in southern Spain and agricultural factors influencing the distribution and prevalence of vegetative compatibility groups and pathotypes. Phytopathology, 101(3), 304-315.
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Does anyone has tips to maintain Bactrocera olea flies under lab conditions?
How to favor puparial emergence (temperature, D/L conditions, food...) and feed adults to keep them alive as long as possible...?
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With an atificial diet rich in protein:
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We are having reference plants for 5 olive varieties. Now we have to differentiate these varieties at nurseries level. The plants are one year old and grown in different media and under different conditions. We want to apply lab methods, other then molecular, to identify the varieties. The techniques should be reliable and reproducible. We will highly appreciate suggestions in this regard.
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You must follow molecular technique. This technique is exact and the other ways not help enough for you. You can't find any result by following the biochmical methodes in the young plants.
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Dear colleagues,
Who hes experience wit application of soft ground rock phosphate in organic farming (Commission regulation /EC/ No 889/2008, anex I); includes all kinds of application: meadows, pastures, vineyards, orchards, olive groves !?
Thank you; Ante Biško
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The ability to have solubility of soft ground rock phosphate is highly dependent on soil pH with a pH between 6.0 to 7.3 which means either liming or sulfuring will be needed for the optimization. For greater efficiency the developing of stocks which have adapted mycorrhizae can facilitate the biology needed to optimize the chemistry. Too much phosphate can inhibit the activity of the mycorrhizae. It will be good to start off with soil testing to understanding the soil constraints you might be working with and also refine your nutritional plan by using foliar analysis. The most common negative side effect of over Phosphorus application can be both the inhibition of mycorrhizae and promoting of inhibition of Zinc nutrition. Full scale chemical analysis is useful both in the soil and in the plant foliage to get the nutrition right.
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We measured the level of flavonoid and phenolic compounds in the olive leaf but flavonoid compounds were higher than phenolic compounds while in the literature flavonoid compounds were lower than phenolic compounds. We want to know that this can be correct?
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Flavrnoids are subgroup of phenolic compounds. You must focus in the lecture to see if their any metabolic bathway consumed phenol content. Also make this test again to be sure about your results
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I am trying to test the combined effect of NaCl and boric acid in in vitro shoot proliferation of olive at different levels of boric acid at 2 mM along with 25&50 mM of NaCl in solid olive medium while olive medium already contains 12.40 mg/L of boric acid. I am facing a problem during medium preparation because when I add Boric acid along with NaCl, it doesn’t dissolve and produced precipitates. I have tried different methods by dissolving boric acid in water, alcohol and also tried by preparing mother solution of boric acid and filter sterilized and added after autoclave but the problem persists again. if anyone can please help me out to solve this issue. Thanks in advance
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Thanks sir
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I found this secondary pest on olive trees, it is Thomasiniana oleisuga, this branch was the forth one that was taken. I spary only the key olive pests such as olive fruit fly.
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Hello Hanna:
The predacious mite Pyemotes ventricosus (Newp.), is considered the major natural enemy of Thomasiniana oleisuga (Diptera:Cecydomiade) in Jordan and the Middle East. There is a paper by Shazli and Mostafa that report some larva parasitoids. You can find this article in the following link:
Best regards,
Luis Miguel
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At least in Portugal, there are claims from populations living in areas dominated by these intensive and large-scale olive farms
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About olive knot
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You can culture pure colony in agar plate and keep it at 4°C or you can prepare 50% glycerol stock by adding equal volume of absolute glycerol and culture broth and store it at -80°C.
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i believe that Oleocanthal is Hydrosoluvel and not lipossolúvel , so i would like to quantify this molecule in olive pulp and not in olive oil,i am looking for the laboratory able to do it.
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thank you Naim
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During composting, i need to reduce polyphenols content in Olive mill waste, so i need to know how can i do that effectively to boost composting operation.
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Dear Rachid,
method validation, in general, may prove by comparison of selected performance parameters to certain performance criteria, e.g. legal criteria for the respective sector of food, that the method is, in principle, fit for the purpose. However, it must then be proven in routine by on-going quality control, that the required standards and/or criteria are being met with. This means, that method performance is monitored in routine by checking certain criteria, often those previously chosen during method validation.
In the case of polyphenols in olive mill waste, one criteria may be that polyphenols must be removed from the waste to a certain extent (e.g. as allowed by legislation, or what is reasonable from a toxicological standpoint) that only a certain concentration, expressed e.g. in weight-% or in mg/kg, remains in the waste. This requirement must then be checked periodically during routine extraction. In other words, you check the efficiency of the extraction step which must ensure that the extracted waste is safe for whatever purpose.
Hope this helps,
Johannes
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I will be supervising a high school student for his research. The proposed research is extracting Oleuropein from 5 different Olive tree species and measuring its concentration and microbial effect. The procedure includes HPLC and UV Spectrophotometer absorbance. The challenge is obtaining pure Oleuropein standard solution for measuring absorbance.The student managed to buy Oleuropein tablets from Amazon containing 50% Oleuropein (Image Attached) as stated on the leaflet. Would it be possible to use this kind of tablet and extract Oleuropein and use it as a standard for measuring absorbance?
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You can use preparative HPLC for isolation; however, the Sigma-Aldrich 10 mg reference standard is only $95,50. Use your research resources wisely.
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I was expecting that the pH of raw olive wastewater around 4.2, but it was near about 8.5
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the only answer is that there was a calcium amendment
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Hello everbody,
I would like check the way I use for pollen collection of olive. I appreciate if you share your knowledge and experience about collecting of olive pollens. In which stage of anters you collect, how do you decide collecting and how do you collect?
Thanks in advance!
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In Olive flower opening frequently between 10am -11pm, its desiccation and release of pollen grain occur gradually throughout two or more days in 80% of the flowers . so you can plan pollen collection. For your help i am giving the link.
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In order to track the efficeincy of treatments on virus elimination in plants, I need to check the plasmodesma changes in plant tissues. Is SEM suitable to follow the changes or the more simple microscopes are okay? what is the best protocol for cell fixation in olive samples for SEM?
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you could to try one-way anova in SPSS.
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Olive knot pathogen
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We can't change it with an other fungicide or no in the cas of isolation of savastanoi and how can i store my sampls of olive knot in 5 °c
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We are testing Olive and parsley (as standard) with Partec flow cytometry. It doesn't show 2 peaks most of the times, though I know they will not overlap with each other!
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yes of course. I have set up parsley as an standard for my samples (Olive), when I mix the two samples together in order to find to seperate peak, sometimes it does not show two peaks though I am sure that the two peaks do not overlap with each other. I use Yokoya et al (1999).
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entomology
integrated pest management
What are the modern approaches for contolling olive fruit fly, Bactrocera oleae?
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Dear Dr. Farag Mahmoud,
in my teamwork we’re currently adopting a conservation biological approach against the olive fruit fly, focusing on the ground predators of the overwintering pupae.
Despite this research is at the very beginning, we have recently demonstrated the pupae consumption by rove beetles in the field. You can find more information in the following paper:
Albertini A., Marchi S., Ratti C., Burgio G., Petacchi R., Magagnoli S. (2018). Bactrocera oleae pupae predation by Ocypus olens detected by molecular gut content analysis. BioControl 63: 227-239.
Best,
Alice
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So, this is the first 10 days of monitoring on a daily basis.
Extensive orchard, presence of wild olives, lack of treatments and working lab
The current generation seems to be declining, time to get prepared for the next one. Any suggestions about how to manage it?
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A forecasting model for indication of expected infestation found in:
Then, to control the infestation, have a look at:
Bueno, A.M. and Jones, O., 2002. Alternative methods for controlling the olive fly, Bactrocera oleae, involving semiochemicals. IOBC wprs Bulletin, 25(9), pp.147-156.
Broumas, T., Haniotakis, G., Liaropoulos, C., Tomazou, T. and Ragoussis, N., 2002. The efficacy of an improved form of the mass‐trapping method, forthe control of the olive fruit fly, Bactrocera oleae (Gmelin)(Dipt., Tephritidae): pilot‐scale feasibility studies. Journal of Applied Entomology, 126(5), pp.217-223.
Baratella, V., Pucci, C., Paparatti, B. and Speranza, S., 2017. Response of Bactrocera oleae to different photoperiods and temperatures using a novel method for continuous laboratory rearing. Biological Control, 110, pp.79-88.
Saour, G. and Makee, H., 2004. A kaolin‐based particle film for suppression of the olive fruit fly Bactrocera oleae Gmelin (Dip., Tephritidae) in olive groves. Journal of Applied Entomology, 128(1), pp.28-31.
Petacchi, R., Rizzi, I. and Guidotti, D., 2003. The ‘lure and kill’technique in Bactrocera oleae (Gmel.) control: effectiveness indices and suitability of the technique in area-wide experimental trials. International Journal of Pest Management, 49(4), pp.305-311.
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Hi dear, I"m working the effects of heat stress in Olive (Olea europaea L.) leaves on biochemical compounds such as proline, gelicin betaine and total carbohydrates and even chlorophyles a and b ane total of them, but I want the ranges measured by researchers throught by some articles. I like this project, because i can help for olive growing. Thanks.
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Please find enclosed some interesting PDFs..