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Oenology - Science topic

Oenology are sensory analysis of wine
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Does the winemaking industry currently use enzymes derived from genetically modified organisms?
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The progress of science in the field of oenological yeasts led to the use of GMOs in the field of viticulture being considered by the IOV (International Organization of Vine and Wine) in 2015. This revision was framed within the International Agreement of the Cartagena Protocol on Biodiversity Security. In this Agreement, three branches were defined where recombinant DNA technology could be applied:
1. Plants
2. Yeast
3. Obtaining enzymes from genetically modified organisms It is worth mentioning that in 2003, both Canada and the United States of America accepted the use of these microorganisms, qualified them within the GRAS (Generally Recognized as Safe) category and today they can be acquired commercially.
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Hi there!
I would love to hear opinions or experiences about the recommended temperature for grape juice and wine storage.
I need to store juice and wine samples for further chemical analyses (Somers assay, Solid Phase Extraction separation, HPLC for monomers and mDp).
The samples will be stored for about 6 to 12 months, so I'm concern about the stability of the compound during this period of time.
There are opinions about store the samples at -20 Celsius will compromise the compounds stability, so for this reason it's necessary to store everything at -80 C.
Contrary to this, there are many others of the opinion to store the samples at -20 C it's good enough.
What is your opinion? Can you recommend some papers about temperature storage and stability of organic compounds?
Thanks!
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I have found in books that -40 degree Celsius is the appropriate temperature.
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Hi there!
I would love to hear opinions or experiences about the recommended temperature for grape juice and wine storage.
I need to store juice and wine samples for further chemical analyses (Somers assay, Solid Phase Extraction separation, HPLC for monomers and mDp).
The samples will be stored for about 6 to 12 months, so I'm concern about the stability of the compound during this period of time.
There are opinions about store the samples at -20 Celsius will compromise the compounds stability, so for this reason it's necessary to store everything at -80 C.
Contrary to this, there are many others of the opinion to store the samples at -20 C it's good enough.
What is your opinion? Can you recommend some papers about temperature storage and stability of organic compounds?
Thanks!
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The ideal temperature range for storing red wine is between
(45°F - 65°F ) , wines you'll hold for a year or longer that ideal temperature of 55°F.
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Could a total SO2 level of 12mg/L be the cause of reduction occurring in my wine?
Thank you
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I think 12 mg/L is possible depending on the strain you've used for inoculation. The low H2S strain has a mutation that naturally produces higher levels of SO2/3
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Is it possible to restart a stuck secondary fermentation of sparkling wine? What remedies are there?
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Hi Gemma,
due to the closed system you have in Champagne production there are not many options:
- raise the temperature (remedies might be that then fermentation goes too fast; temperature impact on product)
- improve contact of yeast with product = shake the bottles (cautiously!)
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The base wines underwent malolactic fermentation, some of which did not fully complete it (approximately 60% of the wines). The wines were blended and then bottled ahead of secondary fermentation. How much SO2 would it be advisable to add at tirage to prevent malolactic fermentation from restarting?
Thank you.
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Hello, how much SO2 you need highly depend from pH of your wine.
Higher pH higher amount of SO2 you need. SO2 is more active at low pH values.
pH -3.2 - 3.3 - 50 mg/l SO2
pH - 2.8 - 15mg/l SO2
If pH 3.5 or more you need about 100mg/l because most part of SO2 in this pH value acting with other substances in wine.
See attached picture for more info.
Good luck!
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Is there any newer and more efficient or accurate equipment for measuring the Brix and pH of grape berries than a digital refractometer (Brix) and a pH meter (pH)?
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Interesting. I think no other better method is available.
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Dear Erik, I suppose it is a good idea and a good way to try to remove ochratoxin A in swine, as weel as in wine! (Maybe proposing a glass of wine to the swine coud do the job too, however thats is not a serious proposal for the next research :)
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LOL !
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Hi! I have a list of wine monoterpenes that I want to dose in wine in various concentrations, from 0,2 to 400 μg/L. A liqui/liquid extraction is necessary? Do you have any preparation protocols?
Thanks for your answer!
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Thanks for your reaction! At first time I need to measure the C of terpenes in wine. 
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I have already made some trials based on the protocol of Segarra and al. (1995) which involves the following;
(1) 1mL of sample + 5 mL EtOH 96% v/v + 50uL HCL (1N) and store for 22 hours at ambiant temperature
(2) spin the samples at 10 000 rpm for 10 min
(3) remove the supernatant  and wash the pellet 3 times with ethanol 96% v/v
(4) Dry the pellet
(5) Dissolve the pellet in water.
This method did not give me proper results.
I observed a partial precipitation which is undesirable.
I repeated the method but this time I replaced EtOH by Isopropanol. The precipitation seemed to be much better but now my problem is that after drying the samples in the last step some of the samples still have some colour which would not suit well the following Dubois method which I have to follow . While some samples had color, some others did not dissolve completely and had a sizeable precipitate in water.
A point to note is that all the samples which had precipitate after dissolving in water were the must samples and on the whole wine samples fared better in this method compared to must samples.
I would be grateful if someone could shed some light on my problem and suggest an alternate if possible or a reason to these observations.
Thank for your help
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Take a look at Nunan et al. Plant Physiol. (1998) 118: 783–792 for details on grape polysaccharides. There are many subsequent papers as well. Also, for constituent sugar analysis we use high-performance anion  exchange chromatography (see Nep et al. Food Hydrocolloids 53 (2016) 134-140) for an example.
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Most oak research, looks at short-term levels of oak derived compounds (ie Eugenol, Vanillin, Oak lactones). I can't seem to find a study that goes anything beyond 2 years in bottle.
I am particularly interested in what their contribution is to mature bottle aged aroma.  
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2 years of what? ageing in wood or after 2 years of bottle ageing?
extraction is maximum in vetween 6 and 9 months for volatile phenols lactones and aldehydes. Phenols are very constant with ageing and aledydes are subject to oxidation and then esterification, lactones are unchanged after 2 years but I dont know over this time.
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I am interested in linking firms in resource and waste sharing in order to improve the production system.
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Dear Dr. Chiusano,
your research project actually seems stimulating. I would be grateful if you could keep me updated. If you want, we can discuss on it (who is your PhD supervisor?)Let us keep in contact. (you can also reach me via email)
Regards,
Andrea Raggi
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I am looking to buy an instrument for pressing the juice out of grape samples of small size (up to 1 kg of grapes). As the pressure applies to the grape samples can affect the extraction of the compounds I want to measure, I am looking for tools with pressure control so to be able to standardize the pressing among samples. Any suggestion of suitable instruments? thanks!
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Dear Leo, thanks for your suggestion. I have used hand pressing in the past but I am looking to avoid variability in the samples' composition induced by this procedure. I have found one company in NZ that makes a type of bench press with a manometer for grape processing so the product does exists. However, I am interested in knowing if someone else have come across this issue and how it has been dealt with. Cheers
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What should be the perfect sensory score and biochemical parameters for a best quality wine?
Methodology for sensory evaluation of wine.
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The best method to use is point evaluation system by Fessler, H. J. (1988). Guidelines to Practical Wine making.
APPEARANCE= 3
BOUQUET & AROMA = 6
TASTE= 6
AFTER TASTE=3
OVERAL= 2
TOTAL= 20