Odors - Science topic

I am working with tissue samples from a marine fish that have been preserved in alcohol for 2–4 years. The alcohol has not been changed during this period. I have successfully extracted DNA from some samples using the TIANGEN Blood and Tissue Kit. However, older samples, which appear degraded, molten, and have a strong odor, did not yield any visible bands on gel electrophoresis.

Given the degraded state of these samples, I am looking for an alternative DNA extraction method and kit that would improve DNA recovery. Could anyone recommend a suitable protocol or modifications (e.g., pre-treatment steps, enzymatic digestion, or commercial kits) that have worked for similar degraded tissue samples? Any insights would be greatly appreciated.

For our database of odor responses (http://neuro.uni.kn/DoOR) I received data that uses the different chemical identifiers named above. I am looking for an easy way to look up/convert these into one another.

I already found the ChemCell macro (http://depth-first.com/articles/2010/11/01/chemcell-easily-convert-names-and-cas-numbers-to-chemical-structures-in-excel/) but would be happy to have some R code doing the work.

Where I work, there are three separate rooms for setting up PCR reactions. In room number 2, whener we use UV light for decontamination, there is a particular odor that remains for minutes after opening the door. I have been reading about this, and, apparently, it is ozone. Has anyone here experienced the same? Do you have any thoughts on why does this happen only in one of the PCR rooms? Is it harmful to be in the room smelling this? How can I overcome this issue? Thank you very much!!

Even adding fragrance is not working

Chemical Engineering /Food Engineering

Hi everyone,

I am studying the effects of odor presentation during sleep and wakefulness on psychotherapy outcomes. My experimental design includes: * A between-subject factor: Group (sleep or wake) * Two within-subject factors: Odor (congruent or incongruent) and Time of measurement (pre, post 1, post 2). For all my psychotherapy measures, I conducted a 2x2x3 ANOVA (Group x Odor x Time). However, I found that for some variables, there are significant/marginally significant differences between the wake and sleep groups at baseline (please see the attached figure for an example). To address these baseline differences and run an ANCOVA, I need to identify an appropriate covariate that works for my between-subject factor(sleep-wake). Here are my questions: What can I use as a covariate in this context? Is it correct to think that I need to perform ANOVAs separately for each sleep and wake group, and that I cannot include a covariate in my 2x2x3 ANOVA? I hope I was clear enough. I will be looking forward to your response and I thank you so much in advance!

Hi, have you ever experienced hydrogen sulfide when are you working with anaerobes inside of a Coy anaerobic chamber? Our indicator strip located in our Hydrogen sulfide column removal is already dark brown, we have changed the media recently one week ago and our paper indicator is brown again. How often do you change the media from the cartridge? and have you experienced some odor coming from the inner lock during vacuum and gas purging cycle? do you have any vent in your anaerobic chamber system?

Thank you

My research is all about finding the aroma compounds present in bioethanol and by using activated carbon, I wanted to remove the unpleasant odor present in the bioethanol.

The bioethanol was made from yeast and molasses but it has a strong odor and we wanted to find ways on how to remove it but we must know first what causes its odor.

I am conducting research in which I will be using a plant extract and infusing it into a wet wipe to fight the growth of bacteria and stop the spread of infections. I need to find a way to remove the odor and stain from the leaf extract to be used and infused in the wet wipe.

I am trying to write a systematic review on factors that affect human's discrimination ability of mixed odors. The data obtained from these types of studies will contain categorical data as observations like yes/no. Can I conduct a meta-analysis for such type of data?

As I understood, enzymes can accelerate the conversion of organic waste into CO2 and water, while preventing unwanted reactions leading to foul odor. Please let me know if this is incorrect.

Which waste management industries typically use enzyme-based odor control solutions available in the market for bulk applications?

Note: I am not talking about 'misting systems, which act only on the surrounding air and temporarily mask the odor. Is there a way to stop the odor generation process at the source (solid waste)?

I´m currently in an investigation related to the olfatory system and the possible conexion with empathy in people, so I need a relaible instrument to make the olfatory tests

Asphalt roads can be damaged due to the effects of rainfall due to moisture infiltration, coupled with traffic loads. In areas where asphalt roads are laid especially rural areas where there are animal farms nearby, and cattle drives are common on the roads there is a very high chance that there is dung on the road and it is not cleared for a long time.

What are the effects on the road surface besides causing a runoff with high nutrient content to enter the waterways and causing an odour nuisance?

In the field of environmental protection and conversion of waste into compost, I am looking for a method that can reduce or eliminate leachate and eliminate its odor so that it can be easily used in parks and green spaces.

The true choice olfactometer is the first olfactometer that does not present a gradient of odors to the subject. The test subject is always in a clean air flow until it reaches the choice area. This allows testing of repellents and push pull systems (repellents and attractants at the same time) in the laboratory. It is a development from ingenious Jim Estaver from Sigma Scientific LLC. We have recently started working with our new true choice olfactometer. Who has already gained experience with the system or has already published something? I would be happy if we could share our experiences here.

A few friends and I wrote a paper for the International Journal of Life Cycle Assessment suggesting a list of characterisation factors for odorants. This was an upgrade on the old Heijungs critical volumes approach, because it takes the persistence of odours into account. Our proposal has now been adopted by the Gabi LCA software, so anyone who did the upgrade to version 8.5 can now do rapid assessment of odour impacts without a lot of data entry. (For more info on the method: Peters et al Int J LCA 19(11)1891-1900)

For more context; I am working on an LCA of an innovative pig stable. According to the technology provider, the benefits of using this stable result in reduced "malodorous air" which is good for pig health. Peters et al., 2014 presented a framework to improve odour assessment in LCAs, but I was wondering if there are standardized LCIA methods for odour developed thereafter.

Thank you!

Peters, G.M., Murphy, K.R., Adamsen, A.P.S. et al. Improving odour assessment in LCA—the odour footprint. Int J Life Cycle Assess 19, 1891–1900 (2014). https://doi.org/10.1007/s11367-014-0782-6

Hello, I work with ethylenediamine and I wash carefully my vials with water under a hood and I put them after in an oven, but it seems that there is still a characteristic smell and I worry if it is any dangerous for health over the time.. Have you any idea?

Thank you very much.

Best regards

By using boiling and roasting test,

Artificial nose

I´m working with olfactometry and came across some strange odour threshold results.

All four members in a dynamic olfactometer panel smelled a sample consistently with high and low concentrations but not the concentrations in the mid range. Concentrations ranging from approx. 15 000 to 4 000 x dilution were detected as well as 500 to 250 x dilutions, but two steps in the middle (dilution factors approx. 2 000 and 1 000) were undetected throughout three panel sequences with same four panel members. The noses of trained panel were successfully calibrated with n-butanol shortly before the panel in question. Ascending concentration sequence and yes/no method was used. Enclosed is an example of a panel sequence result.

The sample contained for example bitumen and other (unknown) compounds related to asphalt manufacturing. However, samples from the same (and other corresponding) process containing similar compounds, or actually any other samples I can remember, have not behaved this way ever before, that being said with adequate control database.

I am aware that there are such compounds that behave this way, for example methylmerkaptan and thioacetone. My question is what compounds are "unlinearly" odorous in a described manner; odorous only in certain concentrations, but cannot be detected with human nose in certain others?

I am looking for wind tunnel olfactometer to observe the behaviour of insects towards odour source? Please guide me. Thanks.

I would like to study the impact of the diet on fecal odor in Beagles.

But I don't really sure that phenol, ammoniac, indole are the only species responsible of fecal odor. Moreover it is difficult to assay them because they are not stable. What is the best way.

Thank for your help

I'm confused with the measurement of odor intensity introduced in VDI 3882 Part-1. Should dilution series be presented? Could the panellist just smell the undiluted sample and classify the odor impression with number 0~6? And what does "the series of measurements shall be repeated (at least twice; 3 runs)" mean?

My name is Li Lin, I am a student of SGH Warsaw School of Economics, majoring in International Business. I would like to thank you for kindly participating in the survey. The obtained results will be used for the purposes of my master's thesis “The Impact of Scents on Impulsive Buying Behaviour. The Example of Poland.” It takes less than 3 minutes : )

Wish you all have a lovely Friday and sunny weekend!

Basically, I have formed a blend of synthetic nitrile latex and added tri ethylene glycol ester as Plasticizer, however the odor makes the conditions undesired at the work place. Hence I am trying to such a reagent or any other solution to minimise the odor.

Dear Connections,

I have a sample which has a very strong odor of Kerosene, So I wanna cover this awful odor.

I made a trial using some esters but didn't work.

If there any suggestions, I'll be grateful

Thanks in advance!

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The alkaline stage uses sodium hypochlorite as an oxidant. The particular application involves emissions from the solids management activities at the treatment plant. That is the odorous air sources do not include the liquid treatment parts of the plat. The plant does not have anaerobid digesters. The odorous air results from management of screenings and processing of screenings and sludge from primary and secondary clarifiers.

I know that the use of hypochlorous acid in disnfection of water and wastewater leads to the formation of various halogenated disinfection byproducts that have toxicological concerns. This gives me concern about the use of hypochlorous acid in the odor control scrubber.

There is technical data indicating the efficiency of HOCl in oxidixing reduced odor compounds. But I have not been able to find any information regarding the presence or absence of byproducts of this oxidation process, particularly species such as haloacetics, halates, halites, and haloforms.

Is anybody aware of any information on this topic?

I am looking for the best solution for septage odor control in a pretreatment system that designed for septage before discharge to head of municipal wastewater treatment plant. This pretreatment system include an underground reservation tank for septage and then ABR and equalization tank. If there is any exoeriences to odor control for this condition, please participate in this discussion.

Best regards

We are need a cleaning products control bacteria, fungi, and mold which can cause stains, odors or surface damage, through a physical mode of action that neutralizes microbes that come in contact with treated surfaces. and create a durable coating on a surface for extended periods of time.

Applying simulated Environmental Modeling results together with operational information:

Dustfall monitoring programmes

Leak detection and repair programmes

Odour Management Plans

Fugitive Management Plans

Pollution Prevention Plans

We need to remove the odor in a 1:1 blended mixture of pyridine and 2-chloropyridine (calcium tungstate). Some of the other components are Calcium at 24%, Carbon at 4.25% Phosphorous at 2.25% and Tungsten at 33%. The mixture has 46% moisture. We would like to leave some moisture in the material after calcination but definitely need the odor eliminated. Any assistance would be greatly appreciated.

Thank you,

JE

I remember a paper studied the effects of gender of handler on animal (mouse?) showing that male handler has more negative effects on animal (for example, causing animal anxious) compared to female technician. The animal can distinguish man and woman by their odor. This phenomenon disappears if the handler is well wrapped in gown to prevent odor from escaping it.

This paper seems published in Nature Methods. Unfortunately, I cannot find this paper now. Do you happen to know this paper? Thank you!

1. Single pit technology:

Advantages: Simple technology, low (but variable) capital cost

Disadvantages: Flies and odors are normally noticeable, low reduction of pathogens with possible contamination of groundwater, costs to empty may be significant compared to capital cost, and sludge requires secondary treatment and/or appropriate discharge

2. Double pit technology:

Advantages: longer life than the single pit, easier emptying, significant reduction in pathogens, flies and odors significantly reduced, and product can be used as soil conditioner

Disadvantages: Manual removal of humans is required, possible contamination of groundwater, higher capital costs than single pit but reduced operation costs if self-emptied.

3.Anaerobic technologies:

3.1 Septic Tank

3.2 Anaerobic Baffled reactor (ABR)

3.3 Biogas reactor

4. Aerobic technologies:

4.1 Free-water Surface Constructed Wetland

4.2 Horizontal Subsurface Flow Constructed Wetland

4.3 Vertical Flow constructed Wetland

Hi everyone, One type of Natural gas odorization system is drip odorizer. https://cdn.intechopen.com/pdfs/11460.pdf Where can I find more information about mechanism of Drip odorizers? How do they work? How does odorant drop in a line with pressure of 60 or 250 psi without any pump?  Thanks in advance for your attention and for answering the above questions.

Electronic nose uses digital sensors which mimic human nose to identify a particular odour. In what way it finds application in plant pathology. If it can detect odour caused by rotting of a plant part due to a disease how the electronic nose is able to distinguish between odours caused by different plant pathogens

I am a psychology researcher and am interested in purchasing an olfactometer for delivering odor stimuli during experiments with human.

Could you suggest some vendors (doesn't matter in which country) that sell olfactometers?

Thank you!

what are the different chemicals added in the sludge treatment to suppress the odour generated from H2S? I am also looking for the specific reference on lime.

Thanks in advance for your time and reply,

Veena.

I'm formulating a natural deodorant and found that the 5% concentration of NaHCO3, it works great for inhibiting the scent of sweat, but about 15% of subjects get a rash with chronic usage, ie daily for weeks.

Would 1% still have an anti-microbial effect to prevent odor?

I often see very high pH values (often over 9) at Lake Renvick at Icelandic State Park (North Dakota, USA). Is there a correlation between algae growth and pH

Part 5, July 2, 2019 (Lake Renwick at Icelandic State Park) - https://youtu.be/U9MS7mYgOPg

Also this is another reference:

Water of Beira Lake is polluted, from which it gets a bad odour and green colour. But contray to the canal system of Colombo, the lake contains water with a very alkaline pH value got by primary production and continuous bloom. In August it gets its maximum value, but becomes more neutral from top to bottom, pH value decreasing from 10 to 8. Lake water never gets acidic pH.[3]

Conductivity of the water retains fairly stable between 250 μS/cm and 300 μS/cm, with a slight increase from May to August.[3]

Full temperature ranges between 29.3 and 35.4 °C. Average temperature is 31 °C and August is the warmest month. The temperature decreases rapidly 2 or 3 °C, from the surface to 60-100 cm in-depth and remains stable after that.[3]

Dissolved Oxygen generally follows the same trend than temperature in August. Likewise the thermocline, an oxycline appears exclusively in August. Below 1 meter depth, DO is stable at 7 mg/l during May, June and July. Though it is decreased during August from 13 mg/l to 6 mg/l, shallow depth allows preserving a sufficient level of oxygen. But it would be more stressful during dry season.[3]

Salinity is very stable (around 0.12 g/l) over time and along the water column."

- https://en.wikipedia.org/wiki/Beira_Lake#Water_Quality

Water treatment plant systems (WTPS) to supply water need to amount of water inlet. Based on this, the beat water resources to supply is the Lake or Reservoir. Sometimes in spring and summer after increase of water temperature and nutrient Harmful Algae Bloom occurs and high volume of algae inter to the WTPS and cussed to taste and odor and sometimes toxins in drinking water.

I ran an experiment to evaluate fly attraction to a number of bacterial cultures (n=50). Briefly what I did is: 20 male flies were exposed to two odour sources at a time-one containing a bacterial culture and other containing control; number of flies that showed attraction to the culture and control were counted after a certain time. The test was repeated 10 times for one bacterium. Similarly female flies attraction was also tested for the same bacterium. Thus for one bacterium I had 20 replicates in total (10 for males and 10 for females). Now, I want to analyse the data to evaluate which bacterium showed attraction at what extent and how they differ gender-wise. I am not certainly sure which statistical test will be suitable for this purpose. I was thinking about Kruskal-Wallis test but not sure if its the correct one. I am using SPSS. Could you please suggest which statistical test will be suitable to analyze this data set?

Thanks in advance.

To offer a suitable method for minimizing nuisance and pungent odors released from treatment units in a wastewater treatment plant, I should firstly evaluate the current condition of the plant regarding odor emission. For this purpose, I think I must measure the main volatile compounds present in odorous emissions of the WWTP. I reviewed the literature and I figured out that Hydrogen sulfide, Methyl Mercaptan, and Ammonia encompass the major part of the odor in a WWTP. However, I need your technical assistant to pick up there or four main compounds which I have to measure in the units of WWTP. Thank you for your valuable comments.

Hi!

I am trying to find out the complete genetic sequences of Odorant Binding Protein and Heat Shock Protein for an insect on NCBI nucleotide database, but I always end up with their partial mRNA complete codon sequences which contain Thymine in place of Uracil. I also tried searching for these genes in Gene database of NCBI but couldn't succeed. Can anyone please explain the reason behind the presence of T instead of U in mRNA? Also, can anyone provide an alternate way through which I can find the complete genetic sequences of these genes?

Thanking in anticipation.

Odor can be measured with the help of field olfactometer in terms of OU/m3. But the toxicity level or the presence of pollutants has nothing to do with the odor in most of the cases, except the gaseous pollutants with an obnoxious smell and vice versa. Despite the real fact, as per human psychology, we presume if there is an odor there must be toxication of the ambient air which is completely illogical. Please share your views on it and what's the local believe in your area/ country. Also, inform others about it to avoid the myth.

Currently, I'm working on a project for odor removal from medical-grade ethanol for an industrial ethanol plant. The main product of the plant, i.e., 96% ethanol, suffers from an unpleasant smell. My question is that which components are responsible for this bad smell? Is is possible to remove them by conventional distillation?

I know that there are some metabolites from fermentation process that are responsible for this smell.

Reclaimed / re-refined used engine oil gives off a very bitter smell ! If the oil is treated with base the smell gets worse, treatment with acids produces a different bad odour albeit a little sweet !

What causes this ? Are there and solutions for this ?

Need brief answers

What is your initial assessment before performing laboratory tests? In the flood season, the color of river water is blackened, noting that there is no oil pollution and there is no undesirable odor in the water.

Hi Everyone. I wanted to use activated charcoal for an exhaust system on a waste storage room to remove odor. I wanted to know an estimated life span of an activated charcoal. Also would be nice to know an easy way of reactivating for reuse. Thanks

Propane cylinders are regularly purged with methanol to remove any excess moisture. I looking for a method of methanol removal from the cylinder after the moisture is removed as it is adding an odor and decreasing LPG purity.

Does anyone have a method or an idea for a method to remove methanol from these cylinders?

Thank you,

Mariah

I would like to identify the compounds with an unpleasant odor arising in the wastewater treatment plant and sorting of garbage

business. I assume that it will be mainly the sulfur compounds, volatile amines and hydrogen sulfide. Chemically, the concentration of these compounds is very high, therefore the most important thing is to take and concentrate the sample. I would like to make an HPLC determination.Is this possible? Please advise a literature with practical procedures. Thank you very much in advance.

How can I achieve the appetitive training at low cost. or can i make an elevator like structure with things easily found in the lab?

Good morning,

I need to know where I can buy olfactometers, whether in y shape or four ways for entomological use, the idea is that in the odor chamber small samples can be disposed (essential oils)

Thanks,

Victor

Our 100 cmd AOP facility has an influent flow rate of 0.53 cubic meter per minute. The water from lift station of the septic tank passes through three reactor tanks (1.5 cubic meter). The water then passes through Activated carbon and multimedia filters and UV Light. Unfortunately, the effluent is physically in poor quality since it appears yellow and has a strong odor that resembles ammonia.

I'm looking for practical methods to reuse dyeing wastewater up to 90% and good COD/BOD , TSS and odor removal. I'll appreciate if someone helps.

best regards

I tried distilling vanilla beans (hydrodistillation) but did not get any oil after 4 hours. However, the hydrosol had the same odor of vanilla. Is there a way to get the vanilla oil? Thank you!

Hello,

there are some articles about the chemical nature of the characteristic "odor of rain" when rain begins to fall.

Always some chemicals in the soil or surface coming from plants are mentioned as the reason.

I was on a ship crossing the atlantic. 1000 km away from any coast it started raining. And I immediately smelled the typical odor of rain (it was a polymer covered floor).

Has anybody deeper knowledge??

Regards

Lothar

Aldehydes and ketones are responsible for odourization of the edible oil. i am looking into deep that how can we replace chemical process with enzymatic process.

I'm producing value added products from sea cucumbers. The major problem I'm facing is the peculiar odor that can't be accepted sometimes. If anyone applied any methods to remove bad odor from any type of meat please try to help me. I can make trails for my case.

I am using 2-mercaptoethanol and it smells bad. Is there a way to decrease the smell of the solution after adding mercaptoethanol?

Hi please give me any idea about studies about bacteria that does not apply on human and all vertebrates. This is a school science experiment/research.

Ta!

I have cleaned my CO2 incubator. After the few culture, I have noticed that bad odor was generated from my incubator and cells were died. Then, I cleaned by incubator again and thawed new vial of cells. In addition, I used fresh media before putting my cells on the incubator. But I have noticed same things that happened before. To my best knowledge, CO2 level, temperature and RH pan are working properly. I am eagerly waiting for your valuable suggestion. Thanks

Hello, I am a college student, and this year I am working on the final work on eliminating odor on the sewer network. This is mainly H2S elimination. I would like to ask if you have any products, materials that are capable of removing H2S if these products were recyclable would be great. Thank you for your advice

Bc. Radim Žíla

I am running a synthetic step to get Schiff's base today.

Methyl-4-aminosalicylate and benzaldehyde (in 1:1 molar ratio) in presence of 2-3 drops of acetic acid and absolute ethanol as solvent were taken and subjected to reflux for about 4 hrs.

To my surprise, from the reaction mixture, I am getting benzaldehyde odor and there is no precipitate formed from the reaction mixture.

Why the reaction not occured...? Is there any other method to get Schiff's bases of Methyl-4-aminosalicylate...kindly let me know.

Thank you.

How the single transmitter, the same action potential create different perceptions!!!

According to the literature, it is known that:

" Diethylamine is a secondary amine with the molecular structure CH3CH2NHCH2CH3. It is a flammable, strongly alkaline liquid. It is miscible with water and ethanol. It is a colorless liquid which often appears brown due to impurities. It is volatile and has a strong unpleasant odor... "

Does anyone know or suggest me any secure method which can be used to dry diethylamine?

In conditioned odor aversion(COA) test, Lithium Chloride is used as an emetic agent. How do these agents work?

PP co polymer when molded has an odor. We collected yellow color residue left on the injection mold after molding this COPP. The FTIR search library gives good match but compounds identified are not relevant.

It is some straight chain (fatty ester?)) compound but I cannot identify.

I want to know if fatty acids cause polyester garments to emit more odor than nylon garments. Has anyone studied this or is there an article that discusses this?

Thanks,

Albert

Rice is the most important food crop consumed by man in the world. Because of its

importance, rice breeders have been focused to develop rice quality. Rice flavor quality is a

critical breeding objective in that the quality has a significant impact on consumer preference.

Using a dynamic headspace system with Tenax trap, GC-MS, GC-olfactometry (GC-O), and

multivariate analysis, the aroma chemistry of various rice flavor types (aromatic rices, colored

rices, waxy rices) was analyzed and characterized. First, in black rice with a unique flavor, a total

of 31 odor-active compounds were determined. Among them, 2-acetyl-1-pyrroline (2-AP),

guaiacol, indole, and p-xylene largely influenced the difference the aroma in cooked black rice

and white rice. The former two compounds were key aroma compounds responsible for the

unique character of black rice due to lower odor threshold and unique odor description.

Can anyone suggest me how to make T maze in laboratory for Drosophila Olfactory Shock and Odor Assay? I really need it for my research.

I once saw an entire field of ripe grain release odorous volatiles together with particulate material at dawn. Pretty amazing viewing.

Can anyone help me to reduce enzyme odour? Is there any process or any additive to reduce enzyme odour?

Odors emitted from WWTPS contain many types of deleterious gases like H2S, CH4, NH3,....... I want the different processes used to control odor.

http://www.hubbardhall.com/chemical-applications/wastewater-treatment/wastewater-solutions/odor-problems/

Good luck

odor masking using any chemical without affecting the raw material?

Dear All, 

I'm having trouble devising a treatment for ethyl acrylate odor disposal. In my process I use supercritical CO2 for purification, then after some steps, the resulting gaseous CO2 is released, along with some solvents and ethyl acrylate. In this case, would the use of an activated carbon bed work and be safe (ie risk of violent polymerization?) note that the ethyl acrylate quantity is a few ppm.

Thank you

Please answer the question.

I compared extracting flower under 80-100C with untrasonic under room temperature. And I would thought that the later one could be better to keep topnotes of the flower, avoid loss of fragrance and prevent off-odor. However, the results showed that the former heated one had sweeter and richer fragrance, while the later one was much weaker.

I just wonder, without considering heat can speed up the extraction:

1. May the thermal reaction generate some strong fragrance?

2. Do saccharide/sugar/the sweet fragrance or compounds contribute to increase the odor, that is, they would let us feel the same fragrance more richer?

Could anyone explain or provide information? I would appreciate it if you could provide other extraction method or related paper.

Thank you

is there any parameter to determine or comparison of the duration of odor? what are everlasting smells in the world?

I'm looking for essays focusing on odor perception recording methodology or any other essays that mention the details of such recordings in an experiment.