Science topic

Nutrients - Science topic

A nutrient is a chemical that an organism needs to live and grow or a substance used in an organism's metabolism which must be taken in from its environment. They are used to build and repair tissues, regulate body processes and are converted to and used as energy.
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Only soil solution forms will readily available for uptake of crops. What is the purpose for analysis of total nutrients in soil. Total nutrients which includes all forms, as we won't take criteria to know soil health. Then why most PG and Ph.D works are doing analysis for total nutrients? IIT Kanpur Soil testing device, how will get reliable soil test results with out an extractant?
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Dear Nagaraju sir, your example is quite natural. But for application of fertilizers to crops, we will take criteria of available nutrient status, not by total nutrient content. We will schedule fertilizers for crops based upon soil test results of available nutrients only, as of my knowledge.
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Basically, I have high-resolution spatial data for soil properties (pH, crop nutrients, SOM etc). I would like to see if there are shared patterns between these variables. I could make maps of these variables using Arcmap or something, but I know that this isn't enough. I would like to statistically support any shared patterns between variables but am not sure which methods to use... any ideas?
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You can convert the 2D spatial matrix into an one dimensional array and do simply correlation analysis. You can also try to apply krigging techniques and fit different models to your geo spatial data variable (at individual level) and then compare the models to check if they are similar or not similar. You can also try to run Principal Component Analysis. Or you can try to to do nearest neighborhood analysis, or test the distributions in the data set distribution like poisson distribution, binomial distribution, random distribition, normal distribution or uniform distribution. etc and perform a comparison between varibles based on their types or distribution. It all depends on what is your aim? goal ? hypothesis. You can refer to one or my article as followed:
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Managing soil salinity is a major issue in many regions of the world. For instance, this issue is common in Morocco, forcing farmers to employ acid fertilizers to acidify the nutrient solution, albeit this is not always evident. To aid in their absorption, it is required to deliver a number of nutrients by foliar application (Fe, Cu, Zn).
So, if you know any practical answers to this challenge, please share them.
Thank you
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Because most crop plants are sensitive to salinity caused by high concentrations of salts in the soil, salinity is one of the most brutal environmental factors limiting crop plant productivity, and the area of land affected by it is increasing day by day.
 According to Hayat et al., ( 2010 ) Some plant growth-promoting rhizobacteria (PGPR) may stimulate plant growth and development directly by supplying plants with fixed nitrogen, phytohormones, iron sequestered by bacterial siderophores, and soluble phosphate.
 Common bean is mainly grown in poorly irrigated and partly saline conditions throughout the world hence ca be used for your feasibility studies or you can also use Safflower (Carthamus tinctorius L.) which is is a salt-tolerant crop cultivated worldwide.
also check
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Basically, the term fertilizer indicates one or more substance that is added to soil to increase its fertility via replenishing lost essential nutrients required for plant growth and development. Accordingly, the microorganisms included in biofertilizers do not add nutrients, nevertheless, they contribute to the availability of nutrients that are already present in the soil. On the other hand, the application of compost relatively provides various nutrients to the soil. So, compost and microorganisms are fertilizers or not? Also, the composting process is a biological process and the outcome compost contains microorganisms. So, compost is a biofertilizer, is not it?
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Perhaps a rather simplistic comment, but I think that we tend to forget that organic additives also have physical benefits over and above those of fertilizers. These cannot be ignored. For example, the effects of organic additives very often dramatically enhance earthworm activity and infiltration of rainfall. Experimental comparisons must take such effects into consideration. Quite possible that such simplistic issues are more important than microbiological effects, which are so popular these days. Unfortunately earthworm counts mean time spent in the sun and are seldom considered. Charles Darwin would, I'm sure, agree.
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An analytical test was done for the teak leaves of a younger plantation in Sri Lanka. The trees show some symptoms similar to Canker. I want to know if there is any relationship between these symptoms (or Canker) of trees with nutrient deficiencies in the soil. I attached the relevant data for your reference.
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Can you provide some pictures of the affected plants? What sort of symptoms are you seeing that suggest deficiency? NB, the soil is not "deficient" in itself, but has insufficient nutrient X to enable adequate growth of plants. On the other hand, your trees are deficient in nutrient X, if supplementing the soil with X permits their return to healthy growth. Another species may grow perfectly well on the same soil and not suffer the deficiency your trees do as it has other requirements that are not being met.
I can't tell if the "Local" & "Tissue cultured" rows in the table indicates the affected vs unaffected, or similar features on different source plants.
Either way, I couldn't find any direct comparisons with Teak in my copy of Plant Analysis Handbook 3rd edn (Bryson et al 2014), but the N,P,K,Ca and Mg all looked to have similar adequate concentrations in other hardwood species (Quercus, Magnolia etc).
Your trace elements are another matter: Cu seems way out. Most species have up to 20 ppm Cu when adequately supplied. 60-100ppm is either toxicity or contamination in the analytical process. Similarly with Fe, while it can get as high as you have (~200 ppm), most species are listed as adequate at up to 100ppm. Much more than that and I would start to suspect soil and dust contamination. (I am happy to be wrong on that, but experience suggests dust on leaves is extremely contaminating).
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Hi there,
We know that nitrogen is one of the primary nutrients critical for the survival of all living organisms. It is a necessary component of many biomolecules, including proteins, and DNA. All organisms on Earth require nitrogen to survive. Does that mean that all microbes can absorb or assimilate nitrogen from the environment (These include any form of organic or inorganic nitrogen)? If not, where does nitrogen come from in microbes? Thank you very much!
Best wishes,
Quan
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Though, largest pool of freely accessible N2 is atm. N2 but only free living or symbiotic N2 fixer can make use of it. Most of the m. O rely on NH4 and NO3 - N for their growth. The availability of these substrates depend on diverse N2 transforming reactions carried out by nitrifiers and denitrifiers and similar classes inadequate. Microbial N- transforming net works both attenuate and exacerbate human induced global change. They produce and consume powerful green house gas nitrous oxide, lead to eutrophication of aquatic system and at the same time remove nitrogen from water.
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Dear
Can someone provide me the protocol to prepare nutrient solutions (Hoagland or Steiner or any method) for inducing nutrient deficiency in plants?
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What are planning to use for a potting medium ? Are you trying to induce these deficiencies individually or all these deficiencies at once ?
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Biochar have lot of positive effects such as increase PH, carbon sequestration, reduce soil acidity, increase availability of nutrients, Increase cation exchange Capacity in acidic soils, But what is the mechanism to increase CEC by biochar in acid soil?
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Thanks
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I am using hydroponics to study seedling salt-tolerance of rice genotypes but my 15-days old seedlings are showing this symptom. Can anyone please guide me which nutrient deficiency or disease it can be? The symptom is showing in majority of plants grown in both control and stress...
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I agree with Mr Andrew Paul McKenzie Pegman it's probably nitrogen deficit. The plants are weak, the basal leaves are yellowish/brown and the apex is very light in color. Typical characteristics of nitrogen starvation. What culture medium are you using in hydroponics? How much nitrogen are you using?
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In traditional epidemiology studies, we are looking for associations between exposure and outcome. We started with a plausible hypothesis and ended with a conclusion that whether our data support the association. I wonder what the next step would be if the data supported our hypothesis, which means an association was found.
For example, we found a healthy eating pattern is a protective factor for type 2 diabetes. Before we conclude healthy eating will reduce type 2 diabetes caustically. There are several potential plans.
1. Find which food or food combination, nutrients or nutrient combination could lead to lower risk of diabetes.
2. Find a pathway to explain the observed association using mediation analyses based on biomarker, -omics data.
3. Reduce bias in the above E-D associational study, like the measurement error, disease classification.
4. Perform clinical trials to test the association.
I am happy to hear other thoughts.
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Thanks for the answer Cleopatra Katite . I think it is important to discuss the findings with respect to those of previous research as this helps the readers to understand how best to interpret the findings. It would also give the researcher the chance to identify possible sources of bias in their study. Put together, this gives the research context and clarifies the level of generalizability.
Thanks for sharing the question and I hope this helps.
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I want to conduct broiler feeding trial experiment. But I don't have Cro2, Tio2 and other expensive chemicals/reagents to determine nutrient digestibility parameters such gross energy, Metabolizable energy and others.
Can you share me any simple method of determining these parameters by taking samples from fecal samples?
Thank you so much!
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Thank you for sharing the documents and your experience.
I need to know more from your advice about when, at what time of the day and for how many days will I collect the fecal samples to analyze AME, CP, ileal nutrient digestibility and others for broiler experiment conducted for 42 days? Thank you.
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PlantVillage does not contain the data I need
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Check National Research Council (NRC) Text on Nutrient Requirements.
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Hi all,
I am growing tomato plants inside green house applying a nutrient stress to it.
I know that by nutrient stress, some symptoms appear to the tomato plants (e.g. color change).
My humble question is:
Does these symptoms disappear after the application of nutrients? in other words, does the color of leaves change from (e.g. yellow) to normal one?
Thanks
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Hello, I would like to know if anyone can help me or suggest the identification of this bacillus isolated from the soil. It has the photo attached in the normal nutrient medium and photo with Hicrome bacillus Agar Himedia. I'm note sure if it could be Bacillus subtilis. Thanks!!!
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Hello,
Without 16S rRNA gene sequencing it's complicated to di the identification.
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Hey guys, super newbie here, I just started micro and "reconstituted" my E. coli in the nutrient broth instead of the tryptic soy broth as instructed in the lab manual (the video just stated nutrient broth and I was following along) I noticed my mistake when I reread the instructions. Will my bacteria still grow in the NB? Thanks in advance!
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Both are non selective media.Your E.coli will still grow in nutrient agar.
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Hello, I'm conducting a small rhizobox experiment exploring the effects of fertilization on root exudate partitioning. I am planning on using nutrient concentrations as recommended in Hoagland's solution, but I have been having a difficult time finding information on how much solution to add to a soil-filled container. The rhizoboxes are about 655.5cm3, I need to calculate the volume of Hoagland's solution to add to each box.
Thank you!
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Did you ever get an answer to this question? I am looking for this information as well.
I did find this post (https://www.researchgate.net/post/How-to-prepare-Hoagland-solution-as-a-source-of-fertilizer-for-the-soil-medium) which suggests 0.5mL per 500mL of treatment. I've asked the original contributor for her confirmation, but I thought you might have an update yourself?
Thanks
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What is the impact of the single dose of N fertilizer application on the nutrient losses status of rice?
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All fertilizer nutrients are not equally mobile in soils. Under aerobic conditions, relatively mobile nutrient such as nitrate moves deeper into soil profile beyond the reach of the active root system. Under anaerobic puddled conditions with water - table close to soil surface , nitrate nitrogen gets transformed into ammonical form and is absorbed on the negatively charged clay particles, thus not able to move freely with water. This reduces losses of deep placed N. Therefore, it is often recommended for a single deep placement of total N in rice. It seems that it could even be practiced in wheat and other upland crops to harness synergy of nutrient - water interaction for greater photosynthetic activity.
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How does tillage affect soil aggregation and how can structure development affect water movement and nutrient availability?
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Soil tillage can affect the stability and formation of soil aggregates by disrupting soil structure. Frequent tillage deteriorates soil structure and weakens soil aggregates, causing them to be susceptible to decay.
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Are nutrient losses higher under Conservation Agriculture (CA)?
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In some cases, nitrogen losses by volatilization are greater under Conservation Agriculture (CA) since crop residues are left on the soil surface rather than incorporation into the soil. CA promotes the increase in surface soil microbes that will at first use nutrients in the residues and then release them to the plants later as they get recycled. However, since CA promotes growing leguminous cover crops, these systems usually experience a net gain in nitrogen since much of nitrogen is "fixed" from the atmosphere rather than mined from the soil. Furthermore, phosphorus losses due to runoff is decreased under CA since the soil remains covered, infiltration is improved, erosion is decreased and soil organic matter in increased. When applying chemical and organic fertilizers, animal manures and composts to soils under Zero tillage (ZT), these materials should be directly injected or band - applied in order to minimize nutrient losses without significantly disturbing the soil.
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Where nutrients are supplies coming from under Conservation Agriculture (CA) cropping systems?
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To meet crop requirements, farmers can use chemical or organic fertilizers, though decomposing crop residues from green manure cover crops, other cover crops, and previous crops can also provide critical nutrients.
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Getting adequate nutrition can be a challenge as get older. With age, the number of calories need begins to decline. Every calorie consumes must be packed with nutrition in order to hit the mark. In addition, the ability to taste food declines, blunting appetite. Some foods become difficult to chew or digest. Several key nutrients, in particular, may be in short supply as get older.
So, what nutrients and vitamins can benefit us with aging? And how to get enough?
All comments and contributions are welcome.
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Except for the N, remaining all nutrients are from the soil. if the plant absorbs, when we incorporate, they go back to the soil again. in general production it is either loss (when we take the economic part and sell them off) or keeping the same (green manuring). I agree that the microbial population would be good and they help in making the unavailable nutrients into available forms. However, they do not create nutrients. for various reasons recycling is affected and thus soil nutrient loss becoming a perpetual one. How does adding biomass will help in addressing the loss? if we bring from outside, i can understand, additional nutrients are added but in-situ green manuring, how does it will help in generating additional nutrients on the farm? (except for N as many of them are N fixers)
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Mineralisation occur in soil.
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Generally in BOD test for sewage water, it is prescribed to use distilled water for dilution along with addition of various nutrients viz. phosphate buffer, magnesium sulfate solution, calcium chloride solution and ferric chloride solution.
Is it possible to make dilution using tap water if yes or No kindly elaborate.
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No, you cannot add tap water for dilution because the Tap water has TDS. You must be added distilled water for BOD test in domestic sewage.
Wastewater has a BOD higher than the amount of oxygen available in the BOD bottle during the incubation period, the samples must be diluted both for domestic or industrial waste waters. This dilution is done by adding dilution distilled water to the sample in the BOD bottle.
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I am considering using duckweed for research projects. Does anyone have any technical papers that explain culturing and maintaining duckweed that they have used and works?
I am imagining that I can grow it in a small aquarium with a light source. But what about aeration, nutrients, temperature, and filtration?
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In my experience, I did not use aeration to grow duckweed. I used small plastic containers with Hoagland's medium for the nutrient source. For lighting instead of sunlight, I used white LED lights. What must be considered is the surface area of ​​the cultivation space, because duckweed lives on the surface. Make sure there is enough room for multiplication in the surface. Maybe some of my works could help you.
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Need advice on innovative resource-saving technologies for moisture and nutrient storage in saline soils?
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Application of enriched organic matter like vermicompost to increase moisture and nutrient in saline soil with organisms,hormones to promote ecofriendly fertility and productivity of crop.
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Nutrients are vital for the life of plants. How are the available nutrients in plant biomass measured?
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Shoot (or above-ground) biomass (WS) is commonly measured by clipping the vegetation at ground level from randomly selected quadrats, while root (or below-ground) biomass (WR) is extracted from cores or trenches.
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How can the increase/enhancement of new roots (white roots) in Kinnow plants under hydroponic conditions?
I have installed the Kinnow plants in hydroponic condition 50 days ago, Once the plants become yellow but now, all are green and well in condition. I am observing that there are initiated very few new roots which are not up to the mark or no progress in new roots initiation.
which kind of elements/nutrients to use for its enhancement? if any researchers have an experience or idea please may you share it with me.
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Things to pay attention to in hydroponics: 1 The hydroponic container has enough space for root growth, including space for new root growth. 2. For hydroponic nutrient solutions, different formulations can be obtained through literature review. Multiple formulations are compared and pre-experiments are carried out, and the most suitable formula is selected for adjustment before formal experiments. 3. There are root growth in hydroponic container, so it is necessary to shade it from the light, which will also affect the root growth. 4. Replace hydroponic nutrient solution regularly. For example, the hydroponic roots of maize in seedling stage should change water at least twice a week, and use air pump to replenish air.
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Generally, fertiliser companies or even countries, though they mention the moisture percentage, do not mention the particle size of fertilisers in the specification/notification. Even if the nutrients of the plant are okay in quantity what would be the effect on soil and crop performance if the moisture and size are different?
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I want to study the growth kinetics of bacteria against metal nanoparticles, I have immobilized metal nanoparticles on clay. But I am having difficulty in bacterial growth curve that it is not showing antibacterial properties in the form of nanocomposite. Currently I am using LB and nutrient broth for the study.
Thanks for your help
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Dear Noor
I think you should count the bacterial number. Also you can increase the nanocomposite concentration. Optical density (OD) is not the the right way to measure the antimicrobial effect in your case because increasing the nanocomposite concentration will increase the OD. May be counting of bacteria after treatment with different concentration with time will be the best method to study the growth kinetics in your case.
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I am a PhD student in Energy and Environmental Engineering and the field of my research is energy and nutrient recovery from sewage sludge and organic waste.
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Hi Brandon Lee, thank you so much. I will look at it.
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When planting any crop, we notice the growth of various crops accompanying it, competing for nutrients in the soil. What is the solution to get rid of them without affecting the main crop?
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You need to remove weed plants by hand and/ or use a recommended herbicide. Regards.
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How, by measuring the electrical conductivity of the Drainage from the hydroponic growth medium such as cocopeat or perlite, find out how much nutrient solution is needed for irrigation to keep the moisture in the growth medium constant?
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The following details are explaining my experimental design and my doubts about the statistical analysis.
Plant is growing in Nutrient 1 and Nutrient 2 . The toxicants at difference concentrations were (0 to 5%) mixed with nutrient 1 and 2. The experiment is conducted at 5 different temperatures. (Nutrients Vs Concentration Vs Temperature)
In nutrient (1) - concentration of toxicant is 0 (control), 1%, 2%, 3%, 4% and 5% (Treatments). All plants were grown well in all temperature experiments therefore here, I used DMRT to compare the significant results between control (0%) and treatments (1% to 5%).
In Nutrient (2) - concentration of toxicant is 0 (control), 1%, 2%, 3%, 4% and 5% (Treatments). Here the plants fail to grow above 1% therefore, I have only 0% and 1% datas in all temperture experiments. Here, DMRT is not fit to compare the resutls since the datas are fewer than three groups. So, what type of statistics best fit to compared these results (compare 0% with 1%)?
For all temperature experiments, fail to run the two way-ANOVA since the un-even datas in Nutrient 1 (control and 5 concentrations, 1 to 5%) and nutrient 2 (control and 1% concentration). Here what type of statistical analysis is best fit to compare the results ?. The same issue is continuing for three-way ANOVA (nutriens vs concentration vs temperature). Is it possible to compare these three factors by three-way ANOVA or any other statistical methods fit for comparision?
Thanks for your help.
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I generated 2-way design on Genstat(vsni.co.uk) and got following ANOVA output (treatment means & SEDs for further protected t-tests to compare any pair of MEANs). See if you follow the output (layout bad, see attached instead):
>>>
56 "Two-way design" 57 DELETE [REDEFINE=yes] _ibalance 58 A2WAY [PRINT=aovtable,information,means; TREATMENTS=nutmix,Tempp; FACTORIAL=2; FPROB=yes;\ 59 PSE=diff; PLOT=*; COMBINATIONS=present; EXIT=_ibalance] zz; SAVE=_a2save Analysis of variance
Variate: zz
Source of variation d.f. s.s. m.s. v.r. F pr.
nutmix 6 2.06515 0.34419 6.99 <.001
Tempp 4 0.52746 0.13187 2.68 0.039
nutmix.Tempp 24 2.16921 0.09038 1.83 0.026
Residual 70 3.44866 0.04927
Total 104 8.21048
Information summary
All terms orthogonal, none aliased.
Message: the following units have large residuals.
*units* 87 0.462 s.e. 0.181
*units* 90 0.563 s.e. 0.181
Tables of means
Variate: zz
Grand mean 0.476
nutmix 1 2 3 4 5 6 7
0.156 0.431 0.502 0.537 0.532 0.605 0.570
Tempp 1 2 3 4 5
0.527 0.534 0.355 0.434 0.530
nutmix Tempp 1 2 3 4 5
1 0.021 0.097 0.139 0.209 0.312
2 0.599 0.410 0.265 0.388 0.496
3 0.327 0.707 0.576 0.335 0.565
4 0.457 0.695 0.490 0.379 0.662
5 0.547 0.455 0.362 0.901 0.393
6 0.807 0.781 0.355 0.375 0.711
7 0.929 0.594 0.302 0.454 0.571
Standard errors of differences of means
Table nutmix Tempp nutmix
Tempp
rep. 15 21 3
d.f. 70 70 70
s.e.d. 0.0810 0.0685 0.1812
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Are plants able to uptake nutrients as a low molecular mass organic form like Cys and Met, or as attached to DOC or DON, intact??
plants uptake nutrients from the soil solution as minerals, and ions. but what if these nutrients were not in a mineral form, but in a very simple organic form? can the plant uptake the nutrients intact with the low mass simple organic form such as Cys and Met?
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Yes, they can, if you consider phytosiderophores are "small" enough:
See also, my article on phytosiderophores:
I'm sure someone has looked at Cys and Met specifically, but I'm sure it can be found with a google scholar or Web of Science search.
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Dear seniors, I have recently conducted a data analysis on the distribution of nitrogen and phosphorus nutrients.  There are many articles about the significant contribution of nitrogen and phosphorus nutrients to water eutrophication. I also want to know what are the measures to separate nitrogen and phosphorus from lakes or control nitrogen and phosphorus in lakes or related studies?  I have not collected relevant literature, and I hope to be shared by predecessors.  Please instruct.
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Hello, I think it is necessary to grow and dispose of nitrogen and phosphorus-absorbing phytoremediate plants in lakes.
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Why is fertilizer important for feeding the world and why does the application of fertilizers become essential for better crop yield?
Fertilizers are food for plants. Fertilizers replace the nutrients that crops remove from the soil. Without the addition of fertilizers, crop yields and agricultural productivity would be significantly reduced. That's why mineral fertilizers are used to supplement the soil's nutrient stocks with minerals that can be quickly absorbed and used by crops.
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Because ferttlizes are supplying essentionl elements reqired for better crop growth development thereby increading biomass yield
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The measurement of pH is routinely done in soils to interpret and/or predict nutrient availability or a number of biogeochemical processes driving the cycling of phosphorus, nitrogen and carbon. There are a number of probes available which may serve rather robust and easy measurements but it can be also rather frustrating depending on the "status/age" of the probes. However, I wonder if anyone compared measurements done in soil suspensions using CaCl2 solutions (with different soil:solution ratios) compared to direct measurements in (wet) organic soils, i.e. just inserting the probe into the soil. I would suspect that direct measurements in wet soils would result in slightly higher pH values (<< 0.5 pH unit)?! Probably the difference depends also on the soil type itself and some other factors like soil water saturation as well?!
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A colleague just gave me a (overlooked) publication from Nielsen et al. 2017 ( ) which nicely gives an answer on my own question. Others than expected the pH of in-situ measurements was 0.5 to 0.8 lower compared to the slurry measurements in the laboratory. Accordingly, in-situ measurements seems to be indeed the favorable approach at least for more acidic soils as found in pine forests or heathlands in this study from Danish colleagues.
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Hello.
I am studying the variation of nutrient content (especially N and P) in an eutrophicated hypersaline lagoon in Brazil. I would like to better understand the electrochemical behavior of nutrients in water column and/or sediments. This lagoon presents very high oxygented waters, but its sediments exhibit very reducing conditions. If anyone has any suggestion for this issue, it would help me very much. Thank you all.
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I suggest you to carry out some work on sediment oxygen demand (SOD), pore water chemistry (both metals along with P and N), speciation of metals and finally measuring Eh (ORP) of both sediment and pore water. You will get a clearer picture of the aquatic environment that you are working on.
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That is, only synthesize nutrients in laboratories using compounds that are not digestible, if the answer is yes, how is it done, and can it cover all the food demand that a person needs? I would appreciate if you share articles with me!
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Many researchers and people say that the most important parameters to control the nutrient solution in a hydroponic system are electric conductivity and hydrogen potential, but another authors include the solution's temperature and dissolved oxygen as very important. ¿What parameters do you consider? And ¿Why?
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I do agree with Parviz Alizadeh.
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According to inquiries developed so far through Scientific Method, roughly from last 400 years, plants need 17 essential elements to produce and complete its vital cycle, otherwise there will be deficiencies with negative consequences upon productivity or even causing whole death of plant. Overall these elements are divided in two categories: macro (C, H, O, N, P, K, Mg, Ca, S) and micro-elements (Fe, Cu, Mn, Mo, Ni, B, Cl, Zn). Equally, another main concept inside plant nutrition is "The law of the minimum" which states that if one of the essential nutrients named before are scarce, the plant productivity will go down even if remaining nutrients are plenty. This statement is attributed to german chemist Justus Von Liebig in 1840, although, 12 years ago before that (1828), Carl Sprengel, a german agricultural chemist stated that the mineral elements are necessary for life plants and irreplacable. ¿What do you think about? ¿Who's the idea owner?
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Arnon and Stout
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To anyone who happened to grow this strain,
I am using Nutrient Broth to grow it in both liquid and agar. However, after 5 days, I cannot notice a visible concentrating of the cells.
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Hi ,
You can use YPD (yeast extract (1%), peptone (2%), dextrose (2%)) medium and may take 24 / 48hrs to grow.
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I am doing aroid research and several explants already proliferated into shoots and others. Then after several weeks, my explants, the leaves, changed their color into yellowish-green. What makes it change? Is it because of the nutrition compound in the media or the age of the explant or anything? Thank you so much.
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Few factors can contribute to chlorosis. It could be as a result of depletion of chlorophyll or deficiency of iron. Also, it could be as a result of environmental stress.
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What could be the best way to prepare reference soil particularly for soil nutrients. How can we make sure this soil can used for organic matter and nitrogen analysis
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two scenarios:
1) the fertilizers mixed in water and applied to the base of the fruit plant
2) fertilizer nutrients applied through drip irrigation (FERTIGATION)
Q: In which scenario, nutrients will be faster available to the roots of the fruit crops?
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Fertigation process is more effevtive
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Good morning, someone could help me to decipher what is causing this in my solid media, I planted chlorella vulgaris in plate count medium and in nutrient medium and I observed that some of them became fuchsia with the passing of lso days, could it be some metabolite or some microorganism?
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Maybe your plates are contaminated because changes in pH or growth of the microorganism may lead to a change in colour. Also, may I ask what agar are you using and exactly how long have these plates been inoculated. If your cultures were green at first and then turned pink, then it just means that the culture had become old.
To be sure, I suggest you cut a piece of the agar and grind it in some distilled water. Then observe the contents through a microscope. You might get an idea of what happening in case its a contaminant.
Best wishes,
Ritesh
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As the title suggests, I'm looking for references for radish foliar mineral nutrients. I have an experiment in which I have the foliar concentration of N, P, K, Ca, Mg, Cu, Zn, and B. I would like to find the deficiencies limits and excess limits for those nutriments in order to confirm which of my treatment is best suited for that crop.
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Kindly find the attachment. This may help you out.
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what is the effect of 1. macro and micro nutrients, 2. sunlight and precipitation, 3. Canopy management and harvesting regimes, 4. Inter- and Intra-species resource competition (both within bamboo species and between bamboo and trees) on above three parameters?
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Ўсимликларни парваришлашда доим шакл бериб бориш зарур. саралаш нимжон усганларини олиб ташлаш натижада сараланганлари берилган мениралларни ўзлаштиради ва яхши усади.
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URGENT PRACTICAL QUESTION
  • Timing of nitrogen fertilizer application is key in nutrient management in agriculture.
  • Nitrogen is considered the most mobile plant nutrient in the soil, hence timely application is necessary. Phosphatic fertilizer applied during the sowing of seeds is sufficient enough to sustain the seed until spring.
  • N application should best be applied in spring when plant root establishment in the Rhizosphere is at maximum (Shicheyi Philip, 2021).
  • N becomes the most limiting factor even in developed countries, leave alone the smallholder farmers, because of untimely application, with over 70% approximated as wastage due to leakage (Shicheyi, 2021).
  • Until and unless plants have roots they cannot utilize the applied fertilizers (Keshab, 2021). Potassium and phosphatic fertilizers take a little longer time to be ready for the plants. Therefore they must be applied as basal.
  • There should be the wise application of Nitrogenous fertilizers as they are lost through volatilization, leaching, and runoff water. If Phosphatic fertilizer is a complex one with some amount of N, it is not necessary to apply additional N through Urea or other sources (Keshab, 2021). This is the reason, instead of applying 50% N as basal; increase the number of top-dresses at various stages. It will automatically increase the N use efficiency by crop.
  • It must be noted that basal use of N is recommended if and only if it is combined with a urease inhibitor (Shicheyi, 2021).
  • Nitrogen is most needed during a plant's vigorous growth stage which obviously is not basal (Shicheyi, 2021).
  • Moreover, unused Nitrogen in the soil can lead to ammonification which in itself is a source of pollution and CFC.
  • Normally, agriculturists recommend 50, 100, and 100 kg/ha of N, P205and K20, respectively as a basal dose (Keshab, 2021).
The bottom line concept is, 'Supply plant nutrient at the right time in the right quantity and quality."
So:
  • when is that right time?
  • is the basal application of N fertilizer scientifically correct?
Dear RG professionals, could we get references for your valuable answers?
STAY BLESSED!!!
Alem
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You are most welcome dear
Wish you the best always,
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Discuss the main factors affecting the nutrient use efficiency of the crop?
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Nutrient use efficiency (NUE) depends on the plant’s ability to take up nutrients efficiently from the soil, but also depends on internal transport, storage and remobilization of nutrients. NUE is dependent on root growth and root architecture. Endophytic bacteria have a direct influence on root growth and increase nutrient uptake. Genetic variation within and among crops for NUE is well recognized. Genotypic variability, that affects NUE and nutrient uptake, influences some processes and plant mechanisms, including differences in uptake, movement in root, shoot demand, and biomass production. There is a huge range of external factors (such as climate, soil, biological, management practices, etc.) that affects a plant’s ability to take up and utilize nutrients more effectively. In addition, soil acidity, salinity, alkalinity and mineral toxicity also affect NUE.
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Now a days various approaches are used to calculate fertilizer doses for various crops such as blanket, soil testing, plant nutrient testing, soil test crop response etc. All the approaches gives the result based on the nutrient present in the soil. The fixation study can also helpful to estimate the nutrient required to satisfy exchangeable position in soil. Therefore, the quantity of fertilizer required will be sum of the nutrient required to satisfy fixation and crop requirement. In this context fixation study was executed by adding a specific amount of plant nutrients in solution to a specific volume of soil. The plant nutrients were extracted and analyzed using the same procedures used for original routine soil analysis. The data for potassium were generated and fitted with langmuir and freundlich adsorption isotherm and presented in the attached file.
How we can use these data for recommending fertilizer (Potassium) to rice instead of blanket, soil testing and other approaches so as to satisfy the nutrient fixation capacity of soil and to get better and highest yield?.
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I am going to establish a experiment of citrus under hydroponic condition. So, I needed a suggestion regarding that how many days are required to change the nutrient solution (capacity of pot - 25 liter) to avoid any contamination?
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In India the soil health cards are being issued to farmers since last 6 years. However, it is not possible to have soil health cards for each and every field till today. We know that whenever we have a bumper crop of sorghum, maize, rice, wheat etc. the soil nutrient status changes after every crop season. Under such situation it is required to measure the soil nutrient status after every crop season. Therefore, we need to have a fast and accurate measurement system which can provide the soil health card to the farmers in his field itself so that he can plan for fertilizer application rate just before the crop sowing season. Under present laboratory analysis method it takes months to get the soil health card and by that time the crop sowing season is over.
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See also the following very good RG link:
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I have estimated different nutrient concentration present in plant leaf. Is there any method or equation from which I can obtain the nutrient status of stems of same plant?
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I doubt , any such empirical equation is available , where we can derive the shoot nutrient concentration from leaf nutrient concentration . But , nice question.....
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Hello
I am a nutritionist and I would like to calculate some dietary indices like dietary antioxidant index. For this purpose, I should find the global mean and SD of nutrients intakes.
How I can find them?
Thanks
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Cation exchange is an important reaction in soil fertility, in causing and correcting soil acidity and basicity, in changes altering soil physical properties and as a mechanism in purifying or altering percolating waters.
The plant nutrients calcium, magnesium and potassium are supplied to plants in large measure from exchange forms.
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Cation exchange capacity
Cation exchange capacity (CEC) is a useful indicator of soil fertility because it shows the soil's ability to supply three important plant nutrients: calcium, magnesium and potassium.
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lately, I've been thinking about whether or not mycelium can be used to link two brains or a set of neurons together. Mycelium is already known to be able to pass nutrients as well as electrical signals across the ground floor, so I theorize that it could be used to link neurons together or possibly even two brains. interested in what others have to think!
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How long will it take for arthropod ingredients to appear on our menu?
Recently, Nestle has released food for dogs and cats, in which, in addition to the usual chicken, they added chopped fly larvae. And no, the global corporation does not save on cats. Livestock is one of the drivers of climate change, and replacing cows with insects can reduce its turnover. Some insect products have been on the market for a long time. Tell us who you can try and what sensations to expect?
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Depends on the country and culture. Unlikely in Brazil. Most likely in China.
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Potassium (K) is one of the three major plant nutrients and most neglected by the farmers though it considered as equal as or sometimes more than the nitrogen requirement by the crops in many of the Indian soils. Though the potassium exist in different forms viz. water soluble and exchangeable K as available and non-exchangeable, fixed K and clay lattice K as slowly available to plants, the available K constitutes only 1-2 % of total K. In this circumstances, nutrient fixation study may give better idea to quantify the fertilizer to be added to crops. In this aspect I initiated K fixation study through adsorption isomers (Langmuir and Freunlich) for 5 different soils under Tamiraparani river command area. The data and graph pertaining to the K fixation study are enclosed for discussion. Please suggest me the amount of nutrient to be added to satisfy the K fixation.
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It does give ample idea about nutrient behavior under controlled conditions
but relating with field conditions , becones a formidable task to have reproducible results...
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At what concentration shall the stock solution of rifampicin be prepared?
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supplemented with 15 μg/ml of rifampicin
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Currently I am looking for some key literatures (articles, book chapter, thesis, etc.) on how farmers decide on adopting new practices of improved nutrient and carbon management particularly those with strong technological aspects. I would appreciate if relevant experts can suggest some articles/papers on this topic. Thank you for your kind attention.
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Hello all,
I am looking for adaptable interpretation of my data to understand the relationship between species abundance in different communities with soil nutrients (N,P,K and OC). For this I have 5 communities with 3 replicates each. Kindly suggest which multivariate analysis will be most appropriate. Thanks.
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Try Pearson correlation alongside Multiple regression to check the dependent variable (soil nutrients) using the independent variables (species abundance)
Best!!
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Hi,
I hope you are all doing well. I've seen several research publications that use different starter level (concentrations) as research treatment for fermented food products. My first thought would say that it won't matter as much, considering that as long as there's nutrient available and no any inhibiting factors, it will continue to grow until reaching the dead phase. Well, higher starter concentration, theoretically, might results in faster growth, but is that really important in the fermented food product?
Sharing your practical experiences would be very much appreciated.
Many thanks.
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Yeah. Addition of starter rate in the raw material affects the final product. Like, with some changes like pH, acidity, flavour production, proteolysis, improvement in bio-functionality along with rate of inoculation, generation of antioxidants.
These all depends on rate of addition, period of incubation, and temperature of incubation.
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I am trying to introduce mushrooms in aquaponics and see if they can purify the water or add nutrients to it. Does anyone have any advice or suggestions that can be relevant to this research?
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Check this paper we made on how to improve the biosorpion capacity of submerged mycelia.
cheers
Francisco
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I've come accross many sources which states that Ni is an essential micronutirent, but Hoagland's solution does nit contain Ni. Also, I've cultivated alot of plants in Hoagland's solution (without Ni). I figured there must be some trace contaminents in the stock nutrient salts, but haven seen any? So do plants really need Ni?
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I am gathering data from papers and some authors present the availability of nutrients as kg/ha and others as mg/L. Would that be possible to convert mg/L to kg/ha once L is volume and ha is area?
PS.: It is not that common to find the length and width of the ponds, but they always present m2 or ha and depth.
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I have a strange problem.
I prepared 500 ml of nutrient agar and 500 ml of Sabouraud.
After autoclaving, I pour them in petri dishes but many plates still semi liquid .. !!
I repeate the process for several times and the problem still occured.
Why..?
and how I solve it?
Is the problem may be ..
Manual mixing?
Programming of the autoclave?
Any source of vibration before solidification?
or other reasons?
Also, I used 3 ml of agar agar to solve this problem but it doesn't work.
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For 500 ml Nutrient agar, you will need to add 15-16 g of Agar. First microwave the media till the agar is dissolved and then autoclave it. Hope this helps.
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Someone help me thanks a lot
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Have you ever used dilution and plating methods for this? These methods are much useful in culturing soil
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We have seen many coral reefs. Some of these are algae staple because they are rich in nutrients. However, in such a situation we observed a coral reef dominated by Sponges. Physical and other parameters of the water indicate that it should also be an algae-dominated reef rather than a sponge-dominated coral reef. We can't understand why this happened.
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It may be related to the luminosity in the location. Sponges may be in advantage on low light environments.
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I have few young mango seedlings that I had moved to the ground way too early. My soil is calcareous and very alkaline. I have been trying several combinations of fertilizers but I apparently made things worse.
This time I'm interested in finding out the deficiencies that cause new buds to cease growing and freeze. Giving a balanced fertilizer (18-18-18 with chelated micros didn't help).
Looking at the nutrients responsible for cell generation/elongation made me focus on Boron/Calcium/P maybe/Zn?
Could it be that some seedlings just won't respond well in very alkaline soils?
The soil as the test shows is mostly calcium but I would take that with a grain of salt, as multiple fertilizer applications/irrigations and the layers of vegetable compost must have changed a lot since then.
Can you think of anything else please? More precisely the nutrients that are crucial for cell generation and elongation. Also the ones that are more likely to suffer when overfeeding NPK.
I find this one very tricky as it seems to be an extra silent deficiency where symptoms are hardly visible.
Let me know your thoughts please. Thanks
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Thanks Kouadri Mohamed El Amine I have. Seaweed extract/FA/HA/Amino acids.
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I am looking to prepare Nutrient Agar and Mueller Hinton Agar by combining the broth versions with agar. How much should I add per liter? Or which is the proportion of broth to agar?
Thanks
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Bonjour, svp comment puis-je diluer mes amorces lyophilisées qui sont à 25 nmol pour avoir des concentrations finales de 0,2 pmol/ul, 0,4 pmol/ul et 0,5 pmol/ul.
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It is already proved that nano-fertilizer increased the nutrient use efficiency, crop yield , controlled release of nutrients , increased the microbes and plant activity, help crop to stress tolerant as compared to the chemical fertilizers. Many industries also now come forward to produce nano-fertilizer in commercial scale. Do you feel it is the right time now to replace chemical fertilizer by nano-fertilizer?
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I do not think nano-fertilizers will be able to completely replace conventional fertilizers in the near future. Still, there are many unanswered questions. To me the most important dilemma is how nano-fertilizers will be able to supply the required amount of nutrients in harvested grains on a long-term basis. For example, a crop of irrigated wheat or rice removes more than 100 kg N ha-1. From where this much N is going to come year after year when we will be applying only nano-urea.
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Dear Researchers!
Since Activated Charcoal is an inert solid adsorbent material used in plant tissue culture to improve cell growth and development as well as adsorption of inhibitory substances in the culture medium. It is said that AC alters medium pH to an optimum level for morphogenesis How the addition of inert AC will change the pH of the nutrient medium?.
Thanks in advance...
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The change in pH results from the hydrolysis of sucrose, which could be increased by the presence of AC-
read the following contrasting works
Druart, P., De Wulf, O. Activated charcoal catalyses sucrose hydrolysis during autoclaving. Plant Cell Tiss Organ Cult 32, 97–99 (1993). https://doi.org/10.1007/BF00040122
and
Wann, S.R., Veazey, R.L. & Kaphammer, J. Activated charcoal does not catalyze sucrose hydrolysis in tissue culture media during autoclaving. Plant Cell, Tissue and Organ Culture 50, 221–224 (1997). https://doi.org/10.1023/A:1005947008637
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I prepared the nutrient broth and revive the bacteria (C.V) using the stock culture of C.V. The culture was 10-15 days old and bacteria revived in the broth.
After 25 days, I tried to revive the bacteria on the nutrient agar plate by streaking method but no growth was observed on the agar plate. Then I tried to revive the bacteria in broth from the same stock culture but there was no bacterial growth in the broth also.
But on the stock culture plate, there is good growth of bacterial colonies. So what could be the probable reason that bacteria not revived in the broth as well as nutrient agar?
Kindly share your suggestions.
Thanks
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I prefer to grow it on Nutrient Broth.
I think it is better before growing on NA.
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We know neutral pH allows the highest availability but I want to clarify myself about the influence of pH on nutrient availability of plants.
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This is such a question that you browse this issue on any search engine, there will be pouring literature. An issue which makes the basis soil fertility and plant nutrition...