Nutrients - Science topic

Dear Nagaraju sir, your example is quite natural. But for application of fertilizers to crops, we will take criteria of available nutrient status, not by total nutrient content. We will schedule fertilizers for crops based upon soil test results of available nutrients only, as of my knowledge.

You can convert the 2D spatial matrix into an one dimensional array and do simply correlation analysis. You can also try to apply krigging techniques and fit different models to your geo spatial data variable (at individual level) and then compare the models to check if they are similar or not similar. You can also try to run Principal Component Analysis. Or you can try to to do nearest neighborhood analysis, or test the distributions in the data set distribution like poisson distribution, binomial distribution, random distribition, normal distribution or uniform distribution. etc and perform a comparison between varibles based on their types or distribution. It all depends on what is your aim? goal ? hypothesis. You can refer to one or my article as followed:

Because most crop plants are sensitive to salinity caused by high concentrations of salts in the soil, salinity is one of the most brutal environmental factors limiting crop plant productivity, and the area of land affected by it is increasing day by day.

 According to Hayat et al., ( 2010 ) Some plant growth-promoting rhizobacteria (PGPR) may stimulate plant growth and development directly by supplying plants with fixed nitrogen, phytohormones, iron sequestered by bacterial siderophores, and soluble phosphate.

 Common bean is mainly grown in poorly irrigated and partly saline conditions throughout the world hence ca be used for your feasibility studies or you can also use Safflower (Carthamus tinctorius L.) which is is a salt-tolerant crop cultivated worldwide.

also check

Perhaps a rather simplistic comment, but I think that we tend to forget that organic additives also have physical benefits over and above those of fertilizers. These cannot be ignored. For example, the effects of organic additives very often dramatically enhance earthworm activity and infiltration of rainfall. Experimental comparisons must take such effects into consideration. Quite possible that such simplistic issues are more important than microbiological effects, which are so popular these days. Unfortunately earthworm counts mean time spent in the sun and are seldom considered. Charles Darwin would, I'm sure, agree.

Can you provide some pictures of the affected plants? What sort of symptoms are you seeing that suggest deficiency? NB, the soil is not "deficient" in itself, but has insufficient nutrient X to enable adequate growth of plants. On the other hand, your trees are deficient in nutrient X, if supplementing the soil with X permits their return to healthy growth. Another species may grow perfectly well on the same soil and not suffer the deficiency your trees do as it has other requirements that are not being met.

I can't tell if the "Local" & "Tissue cultured" rows in the table indicates the affected vs unaffected, or similar features on different source plants.

Either way, I couldn't find any direct comparisons with Teak in my copy of Plant Analysis Handbook 3rd edn (Bryson et al 2014), but the N,P,K,Ca and Mg all looked to have similar adequate concentrations in other hardwood species (Quercus, Magnolia etc).

Your trace elements are another matter: Cu seems way out. Most species have up to 20 ppm Cu when adequately supplied. 60-100ppm is either toxicity or contamination in the analytical process. Similarly with Fe, while it can get as high as you have (~200 ppm), most species are listed as adequate at up to 100ppm. Much more than that and I would start to suspect soil and dust contamination. (I am happy to be wrong on that, but experience suggests dust on leaves is extremely contaminating).

Though, largest pool of freely accessible N2 is atm. N2 but only free living or symbiotic N2 fixer can make use of it. Most of the m. O rely on NH4 and NO3 - N for their growth. The availability of these substrates depend on diverse N2 transforming reactions carried out by nitrifiers and denitrifiers and similar classes inadequate. Microbial N- transforming net works both attenuate and exacerbate human induced global change. They produce and consume powerful green house gas nitrous oxide, lead to eutrophication of aquatic system and at the same time remove nitrogen from water.

What are planning to use for a potting medium ? Are you trying to induce these deficiencies individually or all these deficiencies at once ?

Thanks

I agree with Mr Andrew Paul McKenzie Pegman it's probably nitrogen deficit. The plants are weak, the basal leaves are yellowish/brown and the apex is very light in color. Typical characteristics of nitrogen starvation. What culture medium are you using in hydroponics? How much nitrogen are you using?

Thanks for the answer Cleopatra Katite . I think it is important to discuss the findings with respect to those of previous research as this helps the readers to understand how best to interpret the findings. It would also give the researcher the chance to identify possible sources of bias in their study. Put together, this gives the research context and clarifies the level of generalizability.

Thanks for sharing the question and I hope this helps.

Thank you for sharing the documents and your experience.

I need to know more from your advice about when, at what time of the day and for how many days will I collect the fecal samples to analyze AME, CP, ileal nutrient digestibility and others for broiler experiment conducted for 42 days? Thank you.

Check National Research Council (NRC) Text on Nutrient Requirements.

Thanks Paul Reed Hepperly

Hello,

Without 16S rRNA gene sequencing it's complicated to di the identification.

Both are non selective media.Your E.coli will still grow in nutrient agar.

Hi Shawnee Kasanke,

Did you ever get an answer to this question? I am looking for this information as well.

I did find this post (https://www.researchgate.net/post/How-to-prepare-Hoagland-solution-as-a-source-of-fertilizer-for-the-soil-medium) which suggests 0.5mL per 500mL of treatment. I've asked the original contributor for her confirmation, but I thought you might have an update yourself?

Thanks

All fertilizer nutrients are not equally mobile in soils. Under aerobic conditions, relatively mobile nutrient such as nitrate moves deeper into soil profile beyond the reach of the active root system. Under anaerobic puddled conditions with water - table close to soil surface , nitrate nitrogen gets transformed into ammonical form and is absorbed on the negatively charged clay particles, thus not able to move freely with water. This reduces losses of deep placed N. Therefore, it is often recommended for a single deep placement of total N in rice. It seems that it could even be practiced in wheat and other upland crops to harness synergy of nutrient - water interaction for greater photosynthetic activity.

Soil tillage can affect the stability and formation of soil aggregates by disrupting soil structure. Frequent tillage deteriorates soil structure and weakens soil aggregates, causing them to be susceptible to decay.

In some cases, nitrogen losses by volatilization are greater under Conservation Agriculture (CA) since crop residues are left on the soil surface rather than incorporation into the soil. CA promotes the increase in surface soil microbes that will at first use nutrients in the residues and then release them to the plants later as they get recycled. However, since CA promotes growing leguminous cover crops, these systems usually experience a net gain in nitrogen since much of nitrogen is "fixed" from the atmosphere rather than mined from the soil. Furthermore, phosphorus losses due to runoff is decreased under CA since the soil remains covered, infiltration is improved, erosion is decreased and soil organic matter in increased. When applying chemical and organic fertilizers, animal manures and composts to soils under Zero tillage (ZT), these materials should be directly injected or band - applied in order to minimize nutrient losses without significantly disturbing the soil.

To meet crop requirements, farmers can use chemical or organic fertilizers, though decomposing crop residues from green manure cover crops, other cover crops, and previous crops can also provide critical nutrients.

Mineralisation occur in soil.

No, you cannot add tap water for dilution because the Tap water has TDS. You must be added distilled water for BOD test in domestic sewage.

Wastewater has a BOD higher than the amount of oxygen available in the BOD bottle during the incubation period, the samples must be diluted both for domestic or industrial waste waters. This dilution is done by adding dilution distilled water to the sample in the BOD bottle.

In my experience, I did not use aeration to grow duckweed. I used small plastic containers with Hoagland's medium for the nutrient source. For lighting instead of sunlight, I used white LED lights. What must be considered is the surface area of ​​the cultivation space, because duckweed lives on the surface. Make sure there is enough room for multiplication in the surface. Maybe some of my works could help you.

📷

📷

Application of enriched organic matter like vermicompost to increase moisture and nutrient in saline soil with organisms,hormones to promote ecofriendly fertility and productivity of crop.

Shoot (or above-ground) biomass (WS) is commonly measured by clipping the vegetation at ground level from randomly selected quadrats, while root (or below-ground) biomass (WR) is extracted from cores or trenches.

Things to pay attention to in hydroponics: 1 The hydroponic container has enough space for root growth, including space for new root growth. 2. For hydroponic nutrient solutions, different formulations can be obtained through literature review. Multiple formulations are compared and pre-experiments are carried out, and the most suitable formula is selected for adjustment before formal experiments. 3. There are root growth in hydroponic container, so it is necessary to shade it from the light, which will also affect the root growth. 4. Replace hydroponic nutrient solution regularly. For example, the hydroponic roots of maize in seedling stage should change water at least twice a week, and use air pump to replenish air.

The entire argument about particle size centers around an increased availability of the nutrient to the plant. Following links, and attached pdfs throw light on effects of particle size of fertilizers on soil and crops:

Dear Noor

I think you should count the bacterial number. Also you can increase the nanocomposite concentration. Optical density (OD) is not the the right way to measure the antimicrobial effect in your case because increasing the nanocomposite concentration will increase the OD. May be counting of bacteria after treatment with different concentration with time will be the best method to study the growth kinetics in your case.

Hi Brandon Lee, thank you so much. I will look at it.

You need to remove weed plants by hand and/ or use a recommended herbicide. Regards.

Arash Hashemi

I generated 2-way design on Genstat(vsni.co.uk) and got following ANOVA output (treatment means & SEDs for further protected t-tests to compare any pair of MEANs). See if you follow the output (layout bad, see attached instead):

>>>

56 "Two-way design" 57 DELETE [REDEFINE=yes] _ibalance 58 A2WAY [PRINT=aovtable,information,means; TREATMENTS=nutmix,Tempp; FACTORIAL=2; FPROB=yes;\ 59 PSE=diff; PLOT=*; COMBINATIONS=present; EXIT=_ibalance] zz; SAVE=_a2save Analysis of variance

Variate: zz

Source of variation d.f. s.s. m.s. v.r. F pr.

nutmix 6 2.06515 0.34419 6.99 <.001

Tempp 4 0.52746 0.13187 2.68 0.039

nutmix.Tempp 24 2.16921 0.09038 1.83 0.026

Residual 70 3.44866 0.04927

Total 104 8.21048

Information summary

All terms orthogonal, none aliased.

*units* 87 0.462 s.e. 0.181

*units* 90 0.563 s.e. 0.181

Tables of means

Variate: zz

Grand mean 0.476

nutmix 1 2 3 4 5 6 7

0.156 0.431 0.502 0.537 0.532 0.605 0.570

Tempp 1 2 3 4 5

0.527 0.534 0.355 0.434 0.530

nutmix Tempp 1 2 3 4 5

1 0.021 0.097 0.139 0.209 0.312

2 0.599 0.410 0.265 0.388 0.496

3 0.327 0.707 0.576 0.335 0.565

4 0.457 0.695 0.490 0.379 0.662

5 0.547 0.455 0.362 0.901 0.393

6 0.807 0.781 0.355 0.375 0.711

7 0.929 0.594 0.302 0.454 0.571

Standard errors of differences of means

Table nutmix Tempp nutmix

Tempp

rep. 15 21 3

d.f. 70 70 70

s.e.d. 0.0810 0.0685 0.1812

Yes, they can, if you consider phytosiderophores are "small" enough:

See also, my article on phytosiderophores:

I'm sure someone has looked at Cys and Met specifically, but I'm sure it can be found with a google scholar or Web of Science search.

Hello, I think it is necessary to grow and dispose of nitrogen and phosphorus-absorbing phytoremediate plants in lakes.

Because ferttlizes are supplying essentionl elements reqired for better crop growth development thereby increading biomass yield

A colleague just gave me a (overlooked) publication from Nielsen et al. 2017 ( ) which nicely gives an answer on my own question. Others than expected the pH of in-situ measurements was 0.5 to 0.8 lower compared to the slurry measurements in the laboratory. Accordingly, in-situ measurements seems to be indeed the favorable approach at least for more acidic soils as found in pine forests or heathlands in this study from Danish colleagues.

I suggest you to carry out some work on sediment oxygen demand (SOD), pore water chemistry (both metals along with P and N), speciation of metals and finally measuring Eh (ORP) of both sediment and pore water. You will get a clearer picture of the aquatic environment that you are working on.

Check https://news.mit.edu/2020/faster-protein-synthesis-0528

I do agree with Parviz Alizadeh.

Arnon and Stout

Hi ,

You can use YPD (yeast extract (1%), peptone (2%), dextrose (2%)) medium and may take 24 / 48hrs to grow.

Few factors can contribute to chlorosis. It could be as a result of depletion of chlorophyll or deficiency of iron. Also, it could be as a result of environmental stress.

Check https://www.ecofarmingdaily.com/build-soil/soil-fertility/amp/

Fertigation process is more effevtive

Hello Alejandra Vásquez

Maybe your plates are contaminated because changes in pH or growth of the microorganism may lead to a change in colour. Also, may I ask what agar are you using and exactly how long have these plates been inoculated. If your cultures were green at first and then turned pink, then it just means that the culture had become old.

To be sure, I suggest you cut a piece of the agar and grind it in some distilled water. Then observe the contents through a microscope. You might get an idea of what happening in case its a contaminant.

Best wishes,

Ritesh

Kindly find the attachment. This may help you out.

Ўсимликларни парваришлашда доим шакл бериб бориш зарур. саралаш нимжон усганларини олиб ташлаш натижада сараланганлари берилган мениралларни ўзлаштиради ва яхши усади.

You are most welcome dear

Dr Alem Redda .

Wish you the best always,

Nutrient use efficiency (NUE) depends on the plant’s ability to take up nutrients efficiently from the soil, but also depends on internal transport, storage and remobilization of nutrients. NUE is dependent on root growth and root architecture. Endophytic bacteria have a direct influence on root growth and increase nutrient uptake. Genetic variation within and among crops for NUE is well recognized. Genotypic variability, that affects NUE and nutrient uptake, influences some processes and plant mechanisms, including differences in uptake, movement in root, shoot demand, and biomass production. There is a huge range of external factors (such as climate, soil, biological, management practices, etc.) that affects a plant’s ability to take up and utilize nutrients more effectively. In addition, soil acidity, salinity, alkalinity and mineral toxicity also affect NUE.

Also check https://www.mdpi.com/2073-4395/11/2/379/pdf

Please consult this link and PDF

See also the following very good RG link:

I doubt , any such empirical equation is available , where we can derive the shoot nutrient concentration from leaf nutrient concentration . But , nice question.....

Kindly use this

Cation exchange capacity

Cation exchange capacity (CEC) is a useful indicator of soil fertility because it shows the soil's ability to supply three important plant nutrients: calcium, magnesium and potassium.

Check https://sitn.hms.harvard.edu/flash/2019/exploring-the-underground-network-of-trees-the-nervous-system-of-the-forest/

Depends on the country and culture. Unlikely in Brazil. Most likely in China.

It does give ample idea about nutrient behavior under controlled conditions

but relating with field conditions , becones a formidable task to have reproducible results...

supplemented with 15 μg/ml of rifampicin

Also check https://unctad.org/en/PublicationsLibrary/dtlstict2017d5_en.pdf

Try Pearson correlation alongside Multiple regression to check the dependent variable (soil nutrients) using the independent variables (species abundance)

Best!!

Yeah. Addition of starter rate in the raw material affects the final product. Like, with some changes like pH, acidity, flavour production, proteolysis, improvement in bio-functionality along with rate of inoculation, generation of antioxidants.

These all depends on rate of addition, period of incubation, and temperature of incubation.

Check this paper we made on how to improve the biosorpion capacity of submerged mycelia.

cheers

Francisco

Check https://www.researchgate.net/figure/Comparison-of-the-composition-of-Hoaglands-No-2-solution-a-dilute-nutrient-solution_tbl1_40869974/amp

See the following link : https://onlineconversion.vbulletin.net/forum/main-forums/convert-and-calculate/4663-converting-mg-l-into-kg-ha

For 500 ml Nutrient agar, you will need to add 15-16 g of Agar. First microwave the media till the agar is dissolved and then autoclave it. Hope this helps.

Have you ever used dilution and plating methods for this? These methods are much useful in culturing soil

It may be related to the luminosity in the location. Sponges may be in advantage on low light environments.

Thanks Kouadri Mohamed El Amine I have. Seaweed extract/FA/HA/Amino acids.

Bonjour, svp comment puis-je diluer mes amorces lyophilisées qui sont à 25 nmol pour avoir des concentrations finales de 0,2 pmol/ul, 0,4 pmol/ul et 0,5 pmol/ul.

I do not think nano-fertilizers will be able to completely replace conventional fertilizers in the near future. Still, there are many unanswered questions. To me the most important dilemma is how nano-fertilizers will be able to supply the required amount of nutrients in harvested grains on a long-term basis. For example, a crop of irrigated wheat or rice removes more than 100 kg N ha-1. From where this much N is going to come year after year when we will be applying only nano-urea.

The change in pH results from the hydrolysis of sucrose, which could be increased by the presence of AC-

read the following contrasting works

Druart, P., De Wulf, O. Activated charcoal catalyses sucrose hydrolysis during autoclaving. Plant Cell Tiss Organ Cult 32, 97–99 (1993). https://doi.org/10.1007/BF00040122

and

Wann, S.R., Veazey, R.L. & Kaphammer, J. Activated charcoal does not catalyze sucrose hydrolysis in tissue culture media during autoclaving. Plant Cell, Tissue and Organ Culture 50, 221–224 (1997). https://doi.org/10.1023/A:1005947008637

I prefer to grow it on Nutrient Broth.

I think it is better before growing on NA.

This is such a question that you browse this issue on any search engine, there will be pouring literature. An issue which makes the basis soil fertility and plant nutrition...