New Media - Science topic

Hi all,

I am having trouble deciding if this is cell contamination or something else. I keep seeing this fiber like bodies in my media (not on the same level as my adherent cells). There is no smell or media color change that indicates contamination. I do also see these fibers when i attempt to do a cell count with trypan blue. Has anyone seen something similar? I dont see any movement at all and they dont move when I jiggle the flask gently. I am concerned since I keep seeing these even after I rinse with PBS and place in new media. Is something off with my sterile filter when making my media or is this some type of yeast or bacterial contamination?

The market of information and marketing services on the Internet is developing dynamically.

First of all, large technology companies, owners of information portals and social media prevailing on these Internet markets based their business concepts on the provision of information and advertising services through the websites of their online portals.

However, it is still possible to analyze the division of marketing instruments into traditional ones and implemented in new online media in correlation with specific types of advertising in particular types of media for products and services.

In view of the above, the current question is: What types of products or services are already advertised in your countries in new online media, including on social networking websites?

Please, answer, comments.

I invite you to the discussion.

Thank you very much.

Best wishes.

Dariusz Prokopowicz

Hi, everyone. I have some issues with THP-1 cell culture. I have little experience in cell culture and I hope some of you could share some experiences in culturing this type of cell.

I'm currently trying to grow non-differentiated THP-1 cells for MTT experiments. The chosen media is composed of RPMI-1640, glutamine, antibiotics, sodium bicarbonate and FBS (10%). These cells either don't grow enough or die over the weeks. So here are my questions:

1 - Should I replace the old media with a new one, or just add the new media to the old media? I've had success in the past by just adding new media to the flasks, but for some reason, it stopped working over time.

2 - How many times should I submit these cells to centrifugation? In order to dispose of the old media and add a new one, I need to submit the suspended cells to centrifugation. But in my experience, this process ends damaging cell structure of some of the cells and leaves cell fragments, which could be detrimental to the culture. I'm currently submitting them to 1200RPM, during 7 minutes at 4ºC, once a week.

4 - Should I leave the culture flasks standing on a vertical position? I've seen some people recommending to leave the initial, post thaw, T-25 flasks on a vertical position instead of in a horizontal position. The reasoning behind this is to increase direct contact between cells in a smaller space, as these cells are dependant on each other to grow.

Thanks in advance!

In recent years, there have been an increasing number of examples of situations of deliberate practices in which companies operating social media portals consciously shape the general social awareness of citizens, Internet users through specific information policies applied. The Senate Committees of Inquiry at the U.S. Capitol, which have been taking place for several years, address, among other things, the issue of verifying the use of, for example, algorithms on Facebook platforms that promote certain content, including not only socially positive content, but also socially negative content. The aforementioned algorithms are then changed so that the scale of social negativity is reduced. However, recently there have been an increasing number of similar socially negative cases of algorithms promoting specific political content, e.g. promoting content typical of right-wing political options and limiting the spread of certain social media sites typical of left-wing political content. Thus, these are situations of intentional discrimination against a part of the community of citizens holding certain political views, which the owners of certain companies operating social media portals have deemed to be contrary to the information policy applied in their social media and/or the specific ideology promoted in these media. This type of activity does not correlate with the issue of freedom of speech, unrestricted development of the information society, democracy.

Recently, companies running social media sites have been improving the aforementioned media through the implementation of new Industry 4.0/5.0 technologies, including Big Data Analytics and generative artificial intelligence. The aforementioned technologies can also be used to technically improve the algorithms that control and promote selected content typed and passed on by Internet users, users of the aforementioned online media, which is an important part of shaping information policy in these media.

I have described the issues of the role of information, information security, including business information transferred through social media, and the application of Industry 4.0/5.0 technologies to improve data and information transfer and processing systems in social media in the following articles:

In view of the above, I address the following question to the esteemed community of scientists and researchers:

What do you think about this topic?

What is your opinion on this issue?

Please answer,

I invite everyone to join the discussion,

Thank you very much,

Best regards,

Dariusz Prokopowicz

I would be interested in your opinions and own experiences on Jaron Lanier and Tristan Harris' case on how social platforms and the modern age of (monetized) big data manipulate behavior and deteriorate the ability to think critically in an individual.

I don't mean how sophisticated algorithms suggest the best-placed advertisings, that's a commonly known fact and everybody is aware of that nowadays.

What I mean is the perceived "stupification" of younger generations, fueled by overuse of technology in general, and social platforms in particular. The sophisticated tailoring of those platforms to the short attention spans, the mainly senseless content, the biased bubbles and so on, to me, appear to lessen the critical thinking abilities (and -willingness), paired with the Dunning-Kruger-Effect, that those who are the least informed have overwhelming confidence in their ill-informed opinions.

I have a very strong opinion on this, but it may also be biased by experiences I have made throughout the past years as an educator, and deterred by the sort of students I have worked with. I would like to see a bigger picture, not research trends.

So, what are your subjective impressions on the phone- and social platform addicted young generation and their cognitive abilities, their willingness to achieve excellence, and the influence new media has on them?

I know, what is new media like instagram, facebook, and etc...

but, is there an academic definition of new media even though I have a some diea of what new media is?

Over the past few months, a number of offers of various consulting services, training courses and webinars have appeared on online social media, which initially offer the first trial lessons, webinars, training courses as part of a promotion for free and subsequent ones for a fee. Initially, ebooks are offered free of charge as part of the promotion, which are usually developed with the help of ChatGPT, are of poor content quality, usually containing only general, well-known, popular science knowledge, which can be found independently on the Internet on various websites. Besides, the ebooks created with the help of ChatGPT or other similar intelligent chatbots do not contain all the sources correctly listed. Besides, the resulting ebooks contain many passages, whole sentences, paragraphs taken from other publications that are not shown in the bibliography, and plagiarism thus occurs. In addition, in the field of expertise, there are factual errors and irrational, random, random, combined content from different sources, and thus inconsistent with the facts, ridiculous content and/or descriptions of "fictitious facts" occur. This is because much of the database that constitutes the sources of data and information for ChatGPT is factually outdated, as it dates from late 2021 or January 2022. It may happen that in the ChatGPT-generated text there may be some sensitive data of specific companies or public institutions, which found themselves there accidentally by being mistakenly entered into ChatGPT by an employee of a specific company or institution. In view of the above, there are still gaps in paragraphs in the legal norms defining the rules for the correct use of tools such as ChatGPT, and still the adaptation of legal norms to the rapidly developing technology is often done with too much delay. Besides, popular online social media even feature partly free and partly paid training courses and webinars, where Internet users learn how to create texts for articles, columns, essays, etc., as well as chapters for books, which can be published as ebooks, in a relatively easy way with the help of applications available on the Internet based on generative artificial intelligence technology. Besides, in addition to text, graphics, photos, drawings, etc., which are included in the texts of chapters in ebooks can also be generated semi-automatically in applications based on generative artificial intelligence technology. It also happens that during the aforementioned online trainings and webinars, access is sold to specially created websites that act as intermediary platforms, overlays that contain links to various AI-based web applications, which have been classified in a certain way on a specially created platform and, to make identification more difficult, the links are called by different names relative to the web applications to which they direct. In addition, many of these web-based applications based on generative AI technology are made available on the Internet on the original source sites free of charge. On the other hand, on intermediary platforms created by companies or sole proprietors that contain links referencing these applications, access is paid for and is often sold as part of so-called promotional offers during the conducted, aforementioned online training courses and webinars. Internet users usually learn about such online trainings and webinars from banners and advertising posts posted on popular online social media sites.

I described the key issues of opportunities and threats to the development of artificial intelligence technology in my article below:

OPPORTUNITIES AND THREATS TO THE DEVELOPMENT OF ARTIFICIAL INTELLIGENCE APPLICATIONS AND THE NEED FOR NORMATIVE REGULATION OF THIS DEVELOPMENT

In view of the above, I address the following question to the esteemed community of scientists and researchers:

What do you think about this topic?

What is your opinion on this issue?

Please answer,

I invite everyone to join the discussion,

Thank you very much,

Best regards,

Dariusz Prokopowicz

Children and adolescents represent the youngest generations using online social media. The youth currently attending schools and studying in universities are mainly the so-called Generation Z, who grew up with smartphones equipped with Internet access, including online social media. Today, the children and young people of Generation Z are thus at the greatest risk of being negatively influenced by online social media, including various types of misinformation, fake news, misleading unreliable offers of advertised products and services, influencers and youtubers promoting themselves, etc., which are increasingly appearing in them. In addition, there are many untrustworthy offers of products and services in social media, offers presented by influencers and youtubers, offers presented as part of advertising campaigns conducted in these media, offers promoted through spots, animations and advertising videos, in which generative artificial intelligence technology is increasingly used. It is increasingly common for advertising companies to create AI-generated influencer avatars based on intelligent chatbots for their online advertising campaigns. AI-generated fictional influencer personas look, speak and behave like real people in videos posted on social media. Internet users watching these digital influencers often don't realize that they are watching not real people but digitally generated non-sisterly real characters. In addition, in recent years there has been a growing scale of hate speech most often generated by peers from class, school. The developing hejt was the reason for the increasing scale of child and adolescent suicides in some countries in recent years. In addition, many people, especially young girls, have psychological problems due to a sense of low self-worth and self-esteem which is related to spending a lot of time on online social media and watching influencers promoting certain sublime standards, what is in a certain subculture of youth recognized and promoted as informal canons of beauty, attitudes, possession of certain material goods, etc. In addition, such psychological problems were exacerbated during the Covid-19 pandemic, which was associated with locdowns imposed in some countries on selected sectors of the economy, bans on being in certain types of public places, periodically introduced national quarantines and education conducted remotely in the form of e-learning. In some countries, the scale of such bans and restrictions, which were intended to slow down the transmission of the SARS-CoV-2 (Covid-19) coronavirus, was exceptionally large. This was the case, for example, in the country where I operate. Unfortunately, the mortality rate of citizens during the Covid-19 pandemic in Poland, despite the large-scale introduction of the aforementioned anti-pandemic bans and restrictions, was exceptionally high. Besides, the mentioned anti-pandemic bans and restrictions were introduced without adequate public consultation, on the basis of special legal regulations in the form of laws and ordinances, with the bases of prior research and analysis of the potential negative effects of such controversial measures. At present, in 2024, it is known that in the country in which I operate the negative effects of the aforementioned introduced on a record large scale so-called anti-pandemic measures, including bans, lockdown-type restrictions, periods of national quarantine were much more in comparison with the expected but not realized positive effects. In view of the above, taking into account the available technological solutions and applications offered by ICT service providers, as well as the growing scale of psychological problems of children using smartphones and online social media, it is necessary to improve the computerized systems and applications running on smartphones that enable effectively conducted parental control of what a child does on the aforementioned social media. On the other hand, citizens should influence politicians, and politicians should influence the technology companies that run online social media, so that these companies also take much greater care of the safety of children and young people using these media. The aforementioned technology companies should not treat children and young people merely as potential customers for product and service offers presented during advertising campaigns conducted on social media. The technology companies running these media should not create algorithms that promote posts, posts, comments, banners, animations, videos, etc. that contain negative and socially harmful content. That this is how this kind of media works was proven during the Senate committee hearings of former managers who previously worked at Meta, for example, and developed certain solutions within Facebook and Instagram. TokTok has also grown rapidly in recent years, which also features many examples of disinformation, factoids, posts, memes, entries, banners, videos, etc., containing unreliable and factually incorrect content, as well as many advertisements presenting various product and service offers aimed mainly at children and young people.

I have described the key issues of the determinants of the development of social media with attention to the issue of cyber security and the technologies used Industry 4.0 in my article below:

The postpandemic reality and the security of information technologies ICT, Big Data, Industry 4.0, social media portals and the Internet

I described the key issues of opportunities and threats to the development of artificial intelligence technologies in my article below:

OPPORTUNITIES AND THREATS TO THE DEVELOPMENT OF ARTIFICIAL INTELLIGENCE APPLICATIONS AND THE NEED FOR NORMATIVE REGULATION OF THIS DEVELOPMENT

In view of the above, I address the following question to the esteemed community of scientists and researchers:

And what is your opinion about it?

What is your opinion on this issue?

Please answer,

I invite everyone to join the discussion,

Thank you very much,

Thank you,

Best wishes,

Dariusz Prokopowicz

I ask because different countries have different solutions to this issue. In the country where I have been operating for many years, the main, dominant, meanstream, public news media are under government control, are state-owned (e.g., companies in which the treasury dominates as the main or sole shareholder), are referred to as public, and are used by the government as a mouthpiece for pro-government propaganda, including generating disinformation to citizens so that citizens will once again vote for the same ruling political party in parliamentary elections. On the one hand, this has been operating for many years and nothing has changed in this regard. And on the other hand, it is criticized by media experts, researchers and scholars, by media scholars, but also by many citizens who are not professionally or scientifically involved in the media. The criticism points to the lack of information objectivity, to the politicization of information, to the creation of pro-government propaganda, consisting of constant and incessant praise of what the government is doing, criticism of what the opposition parties are proposing, etc. on the unreliability of information, on the violation of the rules of journalistic ethics, on the public practice of hegemony against people working in opposition political circles, on the practice of disinformation even against people representing the so-called "hardcore electorate" of voters who usually vote in parliamentary and other elections for the ruling PIS political option.

In view of the above, I address the following question to the esteemed community of scientists and researchers:

What do you think about this topic?

What is your opinion on this issue?

Please answer,

I invite everyone to join the discussion,

Thank you very much,

Warm regards,

Dariusz Prokopowicz

On the one hand, online social media, which has been developing for 2 decades now, is making a significant contribution to the development of remote online communication, social remote communication, open communication of sending content created on the fly, sending information to friends, promoting oneself and/or specific product or service offers, informal data transfer, expressing one's emotions in the information sent, including positive as well as negative emotions, and so on. In this way, online social media on a local, regional or global scale have also contributed to the objectification of information in the context of news reported in official, meanstream media. In this regard, online social media are fulfilling their role of social and objectification of media information both in countries with democratic power systems and in non-democratic, dictatorial power systems as long as they are not blocked and restricted by the power system.

On the other hand, there has long been a lot of fake news and disinformation in online social media, the transmission and forwarding by more Internet users of memes, posts, videos, banners, comments containing unverified, unconfirmed content, data and information. In addition, new ICT and Industry 4.0 information technologies, new versions of graphic and other applications are being used to generate disinformation. Also, cyber criminals and hackers breaking into social media user profiles, stealing sensitive data of social media users are also using new technologies. Recently, machine learning, deep learning and artificial intelligence technologies have also been used by cybercriminals and untrustworthy social media users to generate disinformation. As part of this, cybercriminals, hackers and unethical Internet users generating disinformation take advantage of security gaps in information systems, exploit diagnosed inadequacies in cyber security tools operating in the aforementioned social media sites. Thus, it is necessary to conduct relentless monitoring of information traffic on these portals, to identify cybercriminal, hacking, fake news and disinformation generating activities, and to improve cyber security systems, instruments, tools as efficiently and quickly as possible.

In view of the above, what prevails in recent times? Do new technologies rather help cyber criminals, hackers and foul Internet users generating disinformation, or do they rather help the development of social media portals and the improvement of cyber security systems?

In view of the above, I address the following question to the esteemed community of scientists and researchers:

What is your opinion on this topic?

Please answer,

I invite everyone to join the discussion,

Thank you very much,

Warm regards,

Dariusz Prokopowicz

Counting on your opinions, on getting to know your personal opinion, on a fair approach to the discussion of scientific issues, I deliberately used the phrase "in your opinion" in the question.

The above text is entirely my own work written by me on the basis of my research.

Copyright by Dariusz Prokopowicz

Dear all,

I am now starting the HCEC culture with DMEM-F12 media+10%FBS+1%P/S

At the very beginning, cell growth was good after taking out from liquid nitrogen. But after two days, the cell morphology turned terrible and a lot of black dots floated as in the attached pics. May I ask if anyone faced the same problem before? Please kindly suggest some solution to this issue.

In my personal opinion, I would like to re-culture the cell will brand new media with a double dose of antibiotic. Would it be a good solution for this situation? Since I am not quite sure if it is contamination or not.

Thanks, everyone!

What y'all think about this research title? Is there any other better suggestion for the related research field?

Hello, I isolate PMBCs from blood using Ficoll and plate the cells in a 96-well plate for different treatments for a few hours before T cell activation. At the end of the treatments I centrifuge the plate, discard media and use new media to pipette the cells several times and transfer to an anti CD3 coated 96 well plate for 3 days, new media is also supplemented with anti CD28 ab and IL2.

I can't seem to get the cells into the new plate and rarely get any cells in the plate after the 3 day incubation. I was under the impression that these cells detach easily with pipetting. Any suggestions?

Should the development of artificial intelligence be subject to control? And if so, who should exercise this control? How should an institutional system for controlling the development of artificial intelligence applications be built?

Why are the creators of leading technology companies developing ICT, Internet technologies, Industry 4.0, including those developing artificial intelligence technologies, etc. now calling for the development of this technology to be periodically, deliberately slowed down, so that the development of artificial intelligence technology is fully under control and does not get out of hand?

To this, should the development of artificial intelligence be under control? - the answer is probably obvious, i.e. that it should. What remains debatable, however, is how the system of institutional control of the development of advanced artificial intelligence models and algorithms should be structured so that this development does not get out of control and lead to negative consequences that are currently difficult to foresee. Besides, if the question: should the development of artificial intelligence be controlled? - is answered in the affirmative, i.e. YES, then who should exercise this control? So, how should an institutional system of control over the development of advanced artificial intelligence models and algorithms and their applications be constructed, so that the potential and real future negative effects of dynamic and not fully controlled technological progress do not outweigh the positive ones. Well, at the end of March this year 2023, a number of new technology developers, artificial intelligence experts, besides businessmen, investors developing technology start-ups, including, among others, Apple co-founder Steve Wozniak and the founder or co-founder of such technology companies as PayPal, SpaceX, Tesla, Neuralink and the Boring Company, i.e. Elon Musk, Stability AI chief Emad Mostaque (maker of the Stable Diffusion image generator) and artificial intelligence researchers from Stanford University, Massachusetts Institute of Technology (MIT) and other AI universities and labs have called in a joint letter for at least a six-month pause in the development of artificial intelligence systems more capable than the GPT-4 published in March. The aforementioned letter acting as a kind of cautionary petition was published on the Future of Life Institute centre's website, advanced artificial intelligence could represent "a profound change in the history of life on Earth" and the development of this technology should be approached with caution. In this petition of sorts, there are warnings about the unpredictable consequences of the race to create ever more powerful models and complex algorithms that are key components of artificial intelligence technology. The aforementioned developers of leading technology companies suggest that the development of artificial intelligence should be slowed down temporarily, as the risk has now emerged that this development could slip out of human control. The aforementioned petition warns that an uncontrolled approach to AI development risks a deluge of disinformation, mass automation of work and even the replacement of humans by machines and a 'loss of control over civilisation'. In addition, the letter suggests that if the current rapid development of artificial intelligence algorithm systems gets out of hand, then the scale of disinformation on the Internet will increase significantly, the process of work automation already taking place will accelerate many times, which may lead to the loss of jobs for about 300 million people within the current decade and, as a consequence, may also lead to a kind of loss of human control over the development of civilisation. Developers of new technologies point out that advanced artificial intelligence algorithm systems should only be developed when the development of artificial intelligence is under full control, the effects of this development are positive and the potential risks are fully controllable. Developers of new technologies are calling for a temporary pause in the training of systems superior to OpenAI's recently released GPT-4 system, which, among other things, is capable of passing tests of various kinds at a level close to the best results passed by humans. The aforementioned letter also calls for the implementation of comprehensive government regulation and oversight of new models of advanced AI algorithms, so that the development of this technology does not overtake the creation of the necessary legal regulations.

In view of the above, I address the following question to the esteemed community of scientists and researchers:

What do you think?

What is your opinion on the subject?

Please respond,

I invite you all to discuss,

Thank you very much,

Warm regards,

Dariusz Prokopowicz

What are the potential applications of machine learning, deep learning, artificial intelligence, Big Data Analytics and other Industry 4.0 technologies in conducting multi-criteria economic and financial analyses of the historical and current performance of economic entities and making predictions about the future development of their business, analyses carried out on computerised Business Intelligence platforms?

As a result of technological advances, the potential for the application of machine learning, deep learning, artificial intelligence, Big Data Analytics and other Industry 4.0 technologies to perform multi-criteria economic and financial analyses of the historical and current functioning of businesses and to make predictions about the future development of their business, analyses carried out on computerised Business Intelligence platforms, is rapidly increasing. New ICT information technologies and Industry 4.0, including Artificial Intelligence, Machine Learning, Deep Learning, Big Data Analytics but also Data Science, Smart Technologies, Cloud Computing, Machine Learning, Personal and Industrial Internet of Things, Autonomous Robots, Horizontal and Vertical Data System Integration, Multi-Criteria Simulation Models, Digital Twins, Additive Manufacturing, Blockchain, Cyber Security Instruments, Virtual and Augmented Reality and other Advanced Data Mining technologies support the management processes of a company, enterprise or financial institution. In recent years, the aforementioned new technologies are helping to improve the management processes of supply logistics, procurement, production, service offering; marketing communication and customer relationship management; risk management; cyber security management; economic and financial analysis management, financial auditing, etc. Therefore, within the framework of the technological advances taking place, including the increasing computational capabilities of successive generations of processors and operational memory installed in computers, increasing disk capacities, storage media, increasing data transfers, etc., the possibilities of applying artificial intelligence and Big Data Analytics in carrying out multi-criteria economic and financial analyses of business entities, analyses carried out on computerised Business Intelligence platforms, are successively increasing. Consequently, the possibilities for the application of multi-criteria analytics carried out on computerised Business Intelligence platforms are also increasing year on year, which also contributes to the improvement of organisational management processes.

In view of the above, I address the following question to the esteemed community of scientists and researchers:

What do you think about this topic?

What is your opinion on this subject?

Please respond,

I invite you all to discuss,

Thank you very much,

Best wishes,

Dariusz Prokopowicz

The spread of disinformation on social media used by setting up fake profiles and spreading fakenews on these media is becoming increasingly dangerous in terms of the security of not only specific companies and institutions but also the state. The various social media, including those dominating this segment of new online media, however, differ considerably in this respect. The problem is more acute in the case of those social media which are among the most popular and on which mainly young people function, whose world view can be more easily influenced by factual information and other disinformation techniques used on the Internet. Currently, among children and young people, the most popular social media include Tik Tok, Instagram and YouTube. Consequently, in recent months, the development of some social media sites such as Tik Tok is already being restricted by the governments of some countries by banning the use, installation of this application of this portal on smartphones, laptops and other devices used for official purposes by employees of public institutions. These actions are argued by the governments of these countries in order to maintain a certain level of cyber security and reduce the risk of surveillance, theft of data and sensitive, strategic and particularly security-sensitive information of individual institutions, companies and the state. In addition, there have already been more than a few cases of data leaks on other social media portals, telecoms, public institutions, local authorities and others based on hacking into the databases of specific institutions and companies. In Poland, however, the opposite is true. Not only does the organised political group PIS not restrict the use of Tik Tok by employees of public institutions, but it also motivates the use of this portal by politicians of the ruling PIS option to publish videos as part of the ongoing electoral campaign, which would increase the chances of winning parliamentary elections for the third time in autumn this year 2023. According to analysts researching the problem of growing disinformation on the Internet, in highly developed countries it is enough to create 100 000 avatars, i.e. non-existent fictitious persons, created as it were and seemingly functioning thanks to the Internet by creating profiles of these fictitious persons on social media portals referred to as fake profiles created and functioning on these portals, to seriously influence the world view, the general social awareness of Internet users, i.e. usually the majority of citizens in the country. On the other hand, in third world countries, in countries with undemocratic systems of power, all that is needed for this purpose is about 1,000 avatars of these fictitious people with stories modelled, for example, on famous people such as, in Poland, a well-known singer claiming that there is no pandemic and that vaccines are an instrument for increasing control of citizens by the state. The analysis of changes in the world view of Internet users, changes in trends concerning social opinion on specific issues, evaluations of specific product and service offers, brand recognition of companies and institutions can be conducted on the basis of sentiment analysis of changes in the opinion of Internet users using Big Data Analytics. Consequently, this type of analytics can be applied and of great help in detecting factual news disseminated as part of the deliberate spread of disinformation on social media.

In view of the above, I address the following question to the esteemed community of scientists and researchers:

What is your opinion on this topic?

What is your opinion on this subject?

Please respond,

I invite you all to discuss,

Thank you very much,

Warm regards,

Dariusz Prokopowicz

I've been having a lot of issues with my Raw 264.7 cultures--they haven't gotten higher than 60% viability and have slowly decreased in their replication rate and viability. I've used both RPMI and DMEM, both with 10% FBS. I've tried splitting every two days, as well as changing out media with new media every two days and splitting on the third day. I've heard this cell line is pretty sensitive, but I can't figure out how to optimize this culturing to get viability and confluency up to 90%.

Could you suggest any interesting research that measures the cognitive progress of a child (elementary school) by using a tool while teaching (for ex. Videos in education) ? I would like to extent my research on the tools that were used to measure this progress. Thank you in advance.

I'm doing a resazruin-based viability assay. After incubation, I would like to take the media+resorufin and store it e.g. at 4 or -20°C. Then I add new media to the cells and repeat on the next experimental day. At the end, I can read all samples at the same time. Has anybody tried this?

Hi everyone,

I have seen some very strange debris in my U87MG (glioblastoma cell line) culture. It seems that there are shards/fragments of glass that are not contaminating the cell culture and the cells are potentially trying to adhere to them. I attached some pictures of the debris, taken shortly after passaging, so they are not fully adhered.

When I first saw this, I bleached the cells, threw out the package of flasks I was using, made new media, etc. I figured that this originated from a glass pasteur pipette, so I thawed another vial of cells that was frozen down at a different time than the previous ones I used. However, I'm still seeing the same debris in the freshly woken up cells.

I'm confused because I didn't see the debris in the first set of cells until weeks into using them, and I didn't see them in the second set of cells until about two weeks since I thawed them. I may have just not seen it until two weeks in, but it just seems unlikely due to how much and how obvious it is.

This is the only cell line I use EMEM (w/ penstrep & 10% FBS) for, and I have never seen this in any of my other cell types that use other media (completed with the same batch of penstrep and FBS).

Please let me know if you have ever seen anything like this before! Any comments are appreciated - thank you all in advance.

In my opinion, the most recent startups developed in the field of new, online technologies, in the field of new media, business analytics based on automated, computerized, multi-criteria processing of information available on the Internet and providing information and advertising services in new online media.

In view of the above, the current question is: In what areas, branches of modern economies have recently seen the fastest-growing business projects?

Please, answer, comments,

I invite you to the discussion,

Thank you very much,

Best regards,

Dariusz Prokopowicz

Hello all, I am culturing MCF7 cells that are in DMEM/High Glucose media. I split these cells once and the next day noticed these "gold foil"-like residues in the media plate. They seem to be attaching and growing with the cells because every time I wash the plate with DPBS, they go away. Everything in the culture room and culture hood have been cleaned with sterilizers. New bottles of media, DPBS, and trypsin are used. However, once new media is added and the plate is placed back in the incubator, they seem to grow back again. Are these contaminates? I appreciate all feedback!

I'm focusing on changes in the management of media outlets - structure and distribution.

I'm thinking about An Innovation Diffusion Theory and The Post-truth Theory. What else?

How can the development of new online media improve communication between collaborating researchers on an international scale?

The involvement of Internet information technologies, new media for the development of international contacts between scientists improves the logistics of information flow, but does not ensure success in this international cooperation. Online new information media are just a tool that can be used in a variety of ways. Even if Internet technologies of new media are effectively used and advanced information processing technologies are developed, ie typical of the current Industry 4.0 technological revolution, it is still not possible to eliminate all negative aspects, threats and risks resulting from the growing involvement of new online news media to information activities, marketing, research, business and other.

For example, it is not possible to completely eliminate the risk of information transfer on the Internet, including information frauds and deliberate manipulation of information posted on internet information portals and social media portals, information transfer via e-mail etc. carried out by hackers and cybercriminals that break into IT systems of institutions public, financial and business, intercepting and stealing information, making financial embezzlement after hacking into online banking systems.

The most important determinant of the development of international contacts between scientists and researchers are people, their needs in establishing and developing international research teams, including the awareness of the possibility of creating added value, understanding and respect for other cultures and systems of the organization, the need for internationalization of previously determined locally and nationally developed research topics, etc.

On the other hand, social media portals operate as part of the development of new online media. Some information and / or social media portals enable communication between scientists and offer specific information services profiled on scientific issues, research, etc. One of such websites is the Research Gate portal.

Do you agree with me on the above matter?

In the context of the above issues, I am asking you the following question:

Please reply

I invite you to the discussion

Thank you very much

Best wishes

Which social media portals are currently being used to the greatest extent in business, for example in internet marketing, in order to present the offer of products and services, present the mission and perspectives of the company's development, improve the company's brand image?

Please reply

I invite you to the discussion

New, online media, including social media portals, should be engaged to promote the need for development of ecological activities, including the need to protect and develop forests and other issues related to environmental protection, protection of natural, biological ecosystems. Due to the progressing global warming process, with ever-emerging climatic disasters and weather anomalies, with increasingly drought, shrinking arable areas and areas of natural greenery, there is a growing need to increase expenditure on nature conservation and reclamation of areas devastated by human development of civilization .

As the importance of social media portals as sources of information is growing among children and adolescents, these portals of new online media should be used to promote the idea of ​​developing pro-ecological activities, the necessary development and implementation of ecological innovations into economic processes, development of renewable energy sources, excellence in waste segregation techniques , resignation from the use of non-biodegradable plastic from packaging, in addition to activation for the development of recycling, development of organic farming, reduction of greenhouse gas emissions from various production processes, to the development of electromobility, nature protection also in urban parks, and also to promote the idea of ​​area development forestry, afforestation needs of areas degraded by the development of industry and protection of natural sites of biological ecosystems, such as ecosystems of rainforests, including, among others, the largest areas natural forest ecosystems, i.e. rainforests of the Amazon. To this end, social media portals should be increasingly used.

In view of the above, social media portals should be used as key marketing tools in planning and implementing social campaigns that promote the need to protect nature, including natural bilogic forest ecosystems, but also others, such as urban park ecosystems, for example, putting houses for birds and for insects , resignation of grass till the flower meadows needed for pollinating insects, etc. It is also necessary to also promote the protection of aquatic ecosystems, ie ecosystems of rivers, lakes, seas and assessments, which are increasingly polluted and also change biologically under the impact of the ongoing global warming process. Social media portals should be used to promote the need to implement sustainable economic pro-ecological development based on the philosophy of transformation of the classical economy in green economy into economic processes.

Of course, one of the most important elements of the necessary proecological reforms is also the need to protect natural clumping ecosystems, the need for their reclamation and reconstruction in the areas devastated by industrial development and the need for afforestation development both outside built-up areas and in urban agglomeration areas. This type of necessary pro-ecological activities should be promoted on social media portals. The state should finanate this type of social campaigns conducted in new online media, in particular in social media portals. Apart from state institutions, also various non-governmental organizations, social organizations should run such campaigns on social media portals, because such social campaigns conducted through social media portals using viral marketing instruments do not have to require the involvement of large financial resources.

Do you agree with me on the above matter?

In the context of the above issues, I am asking you the following question:

Please reply

I invite you to the discussion

Thank you very much

Best wishes

Hi everyone.

I'm working on my PhD dissertation which focuses on the experience of media journalists on Twitter. Basically, I am going to examine how their production, distribution, and consumption practices have changed in the new media ecology. But I am still undecided over the theoretical framework I should use for the study. I am currently reading on journalists' online experience to see if I can find relevant research.

Do you have any suggestions?

Apparently, in the second half of 2018 Instargram is growing faster than Facebook, taking into account the increase in the number of new users setting up their profiles on individual social media portals.

Do similar trends occur when comparing other social media portals?

Do social media portals dedicated to mobile devices develop faster than other types of social media portals?

Please reply

The problems of the analysis of information contained on social media portals for marketing purposes are described in the publication:

I invite you to discussion and cooperation.

Best wishes

In order to effectively promote the issue of natural ecosystems in the context of developing models of sustainable development, it is first necessary to develop an information program that will act as the content of specific social campaigns conducted in various types of media, including new media, also on social media portals. Both social information campaigns should be conducted on a large scale. In addition, there should be successive changes to the law, ie new legal regulations that will motivate the transformation of business models towards the development of green economy and sustainable development. These campaigns should also motivate research centers to create new ecological innovations and new technological solutions in the field of renewable energy sources.

Please, answer, comments. I invite you to the discussion.

Is it for children and young people to limit, limit the use of smartphones?

Should I fully control the use of smartphones by children and adolescents?

If children or adolescents use smartphones for learning, as a tool to support education processes, communication with schoolchildren and friends, and if these devices use new online media from time to time, this may not be positively assessed. However, if divides or young people from smartphones use many hours a day, among other things, viewing advertisements on social media portals and worthless memes and films, then it can have a destructive effect on the intellectual and psychological development of children and adolescents. In this situation, the use of smartphones by children and teenagers should be limited and controlled by parents, guardians and teachers.

Please reply

I invite you to the discussion

Thank you very much

Best wishes

Researching the phenomenon of the recent SARS protests and allegations and how this was communicated in the media, particularly exploring the communication difference between media outlet in Nigeria to the UK and US. Traditional broadcast vs. news articles.

I am trying to grow motor neurons on coverslips and chamber slides, and every time I pipette out the spent media and pipette in new media with a P1000 pipette, a lot of neurons seem to detach despite careful pipetting techniques.

Is there another way I can change media (instead of using P1000 pipette) without risking so much neuronal detachment from the coverslips?

I would be grateful, if you can recommend some good journals in new media communications. Thanks in advance.

Hello All, I work with INS1 cells and am able to grow properly and passage them. Howeve, I encounter difficulties when I attempt to culture them in 6 to 24 well plates. The primary issue is the cells tend to detach following administration of new media or other solutions, even when I add media to the wall slowly (15-30 sec/well). If cells don't all detach, the ones left over tend to be in the center.

Additionally, I will notice a morphology change in some of the cells that don't detach. They change from a full bodied, translucent cell with extensions to a small, rather opaque rounded cell with little to no extensions.

Has anyone encountered this issue? If so what would you suggest?

I have used poly-d-lysine coating to mitigate this, and while it has helped is hasn't completely erased the problem.

Any help would be greatly appreciated!

Does the influence, type, scope of economic and economic information on the current situation on the financial markets and the condition of macroeconomic determinants describing the entire economy and how they are presented in the media can have a significant impact on investors, especially the so-called small investors, including households, on investment decisions made on the capital markets and thus on the future situation on these markets?

The key issue is social psychology, the psychology of investors operating on capital markets, the ability to influence stock market trends by providing key information in the media on the macroeconomic situation of the country and information provided by rating agencies, large banks and investment funds, central banks, financial supervisors, research institutes and government agencies.

Is it possible to use the available classic and new media, including the Internet by large commercial financial institutions for their own needs, eg attempt to trigger certain changes in stock exchange trends by providing economic information to the media that may affect investment decisions made by investors?

Are there known, diagnosed, investigated such situations?

Do you agree with my opinion on this matter?

In view of the above, I am asking you the following question: Can economic news in the media influence the psychology of investor behavior in the capital markets?

Please reply

I invite you to the discussion

Thank you very much

This issue is described in the following publication:

I invite you to discussion and cooperation.

Best wishes

I am slightly confused between Framing vs Agenda Selection vs Representation. Are there clear examples?

Also, on doing a content analysis based on media framing, how do you identify the frames of the stories in terms of : economic consequences, responsibility, moral impact ...etcis it just mere categorization? Many thanks

I am still trying to get a proper grasp on seeding cells into wells, and I have a formula that indicated I would need ~41 mL of concentrated cells (ST cells in my case). I am using nine 24-well plates to get a total volume of 216 mL.

Here is my formula, and if it seems incorrect or needs editing, please let me know as I am very much trying to learn the proper way of doing this:

So an example of what I would have is:

Does this seem correct? Am I missing something?

For some more context that may be important:

Any help and guidance would be greatly appreciated!

When talking of those who do translation in news media, are we talking about translators who do journalism, or journalists who do translation? How do we differentiate between the various roles that these agents serve in their professional context?

For my new research, I am planning to interview journalistic translators about their experiences in social media. To avoid any confusion between those experiences which directly relate to their translatorial position, and those which belong other professional and non-professional dimensions, I'm thinking how I could define a journalistic translator and their different, overlapping roles and functions, so as to guide the interviews in the relevant direction.

Suggestions (references to read, methodological approaches etc.) are most welcome.

I believe that efficiently conducted marketing online campaigns in social media can already increase the insensitivity of new online media, including social media to traditional communication channels. Effective marketing campaigns on social media before political elections may already have more than 50 percent share. How big it will be depends on the technological possibilities in the field of new online media for a specific electoral staff that is active during the election campaign.

Please, answer, comments. I invite you to the discussion.

Will the development of data processing technology accumulated in the Big Data banking database systems improve the credit risk management process or will it contribute to the development of Shadow Banking and the use of unethical practices for the surveillance of potential borrowers?

Large commercial banks generate high financial surpluses allowing for the implementation of modern integrated teleinformatic internet banking systems, Business Intelligence data analysis systems, data processing platforms in Big Data database systems, etc.

There were already situations of unethical use of modern ICT solutions, analysis of comments on social media portals, during which the bank verified the customer's data entered into the loan application by also scanning information that the potential borrower types in social media portals.

This informal verification took place without the knowledge of a potential borrower and could then be the basis for suing the bank.

However, the bank's client is not always aware of the fact that it can be invigilated in such a way by the public trust institutions that the bank should be.

Of course, these types of cases, which we know from the media is supposedly a margin of entire banking, which can be one of the categories of a new type of unethical practices typical of the so-called Shadow Banking.

However, only part of this type of information gets to the media.

Maybe this is just so-called "the tip of the iceberg" of this problem.

The situation is similar in the situation of cybercriminals' attack on bank IT systems or electronic banking platforms.

If it is possible to keep this type of events secret, then customers do not find out about it.

This is because media only receive information about some of these types of events.

Does any of you conduct research in this area?

If so, I invite you to cooperation.

I am asking for comments

It is said, that New Media transform and revolutionize modern teaching as they improve transmission and distribution of information and knowledge. If it is truth, we should use them.

I am wondering... to what extend and in which form does New Media are used at the University ???

Yes, this is a very important issue. The issue of communication with the use of new online media is very important in the context of an efficiently run education process.

We are currently communicating widely across various online media, including via email. Some of the email mailboxes we use have anti-spam restrictions, which makes communication difficult. The development of communication through various online media, also through social media portals is an important issue in education. New media should be effectively used in the education process, but not always their technical specification is fully suitable for the needs of communication development in the context of the ongoing education process. However, as far as possible, new online media should be used in education, because young people use them widely and can be an excellent additional tool in the field of teaching instruments, eg for the purpose of efficiently searching for the necessary, current information.

In view of the above, I would like to ask you: Should new internet media be used in educational processes?

Please, answer, comments. I invite you to the discussion.

And if so, which types of media are central to this issue?

Please reply.

Hi all,

Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them incubate for another 12 hours to get a 24hour time point, same procedure for another 24 hours to get to 48-hour timepoint or is it necessary to do 3 parallel plates to get 3 timepoints?

Thank you

Is offering free information services on the Internet the main factor in the development of these services? Are there other equally important determinants of the development of online information services? In my opinion, other key factors for the development of online IT services include the technological progress in the field of Internet of Things devices and other technologies of the present fourth technological revolution, known as Industry 4.0.

The current technological revolution described as Industry 4.0 is determined by the development of the following technologies of advanced information processing: Big Data database technologies, cloud computing, machine learning, Internet of Things, artificial intelligence, Business Intelligence and other advanced data mining technologies.

The development of Internet information services is increasingly determined by technological progress in the field of Industry 4.0. Similarly, the development of electronic banking, the development of fintechs and social media portals is also increasingly correlated with technological advances in advanced information processing determined by the development of new Internet information technologies of the current technological revolution Industry 4.0.

Do you agree with me on the above matter?

In the context of the above issues, I am asking you the following question:

Please reply

I invite you to the discussion

Thank you very much

Best wishes

Dear all,

I am looking for projects/initiatives regarding refugee representation and new media technologies. I am interested in researching how refugees use new media technology to represent themselves and how that contrast or not with mainstream representations. Can anyone suggest to me some research on the topic or does anyone know about specific projects/initiatives to check out?

This would be my Master Thesis on Migration Studies and I would really appreciate any advice, suggestion, or observation concerning the topic!

Thank you so much in advance

At present, the dominant opinion is that artificial intelligence will find many applications in IT, teleinformatics, internet new media, robotics, industrial automation etc. in the 21st century.

There are many potential applications. On the other hand, there are research concepts that there will not be one artificial intelligence but many different versions and levels of artificial intelligence.

In view of the above, the current question is: What will be the potential uses of artificial intelligence in the future?

Please, answer, comments. I invite you to the discussion.

I am trying to perform a modified MLR adding CD14+ monocytes to CD4+ T cells (both positive MACS isolation), under different conditions. However at 5 days, even with HEPES in culture media, the wells are yellow and I'm seeing significant CD4+ cell death.

I have been spiking the media with varying volumes of fresh media at intervals throughout 5 days however this doesn't change the outcome much.

Should I spike the wells with new media?

Should I completely exchange the media?

Could this be due to the ratio of APC to responder cells which appears to be crucial (currently 1:1 or 1:2 CD4:CD14)

Any other ideas?

Lastly I am also labelling the CD4 cells with CFSE to check for proliferation, is it ok to do this prior to the 5 days incubation or should this be performed at end of 5 days?

Many thanks

Hatty

Hi there,

I am preparing a workshop about "Science communication: how to use new media to get your research out there". How would you rate your experience with ResreachGate? What are the biggest advantages and disadvantages?

Cheers,

Gloria

Recently, the social media has partnered with World Health Organization (WHO )to create awareness around COVID-19, the latest example of social media, celebrities and influencers being tapped to help fight the outbreak with correct information. now we working on Jordan and UAE -based study, about Social media contribution to awareness on the pandemic, (On the negative and positive side) Do you social media that provides trustworthy information, offers tips on staying safe and preventing the spread of the virus, and dispels myths around COVID-19," ? Do you contribute The social media people influencers to create their own videos that provide users critical and informed medical information and tips.

India has various regional languages. Vernacular newspapers & TV news channels are investing very much in their web editions in India. I want to study the feasibilities of vernacular web journalism, its challenges & opportunities in India.

My Research Title is "Web Journalism : pattern, opportunities & Challenges" (In the context of the web editions of Rajasthan Patrika-Hindi, Ajeet-Punjabi & Lokmat-Marathi.)

1. Is it an appropriate Research Title?

2. Which kind of methodology should be opted for the research.

Looking for guidance & support.

social media, new media tools

A theory which can be related to the area of political communication and mass media. Attachment of any related study in the area would be highly appreciated.

Thank you

Do you think that the new media coverage increases the objectivity of the media and information provided to the public?

However, in recent years, on the basis of the development of behavioral economics, there are more and more data and results of research carried out, that unfortunately there are many examples suggesting the irrationality of some economic decisions.

Often, consumers make unnecessary purchases based on an efficiently carried out advertising campaign for products or services.

Perhaps consumers are more and more often susceptible to the impact of effective advertising campaigns, in addition to traditional media, also in new online media, including on social media portals, where viral marketing is widely used.

Often in advertising campaigns, specific products and services are presented as unique, innovative or having such features as opposed to the substitutional offer of competition.

The message that is not always formulated in the advertising campaign is truthful, but some consumers may receive such a message as objective and this translates into an increase in the sale of a specific, effectively advertised assortment.

Do you agree with my opinion?

Please reply

The problems of the analysis of information contained on social media portals for marketing purposes are described in the publication:

I invite you to discussion and cooperation.

Best wishes

I am a non-microbiologist doing some microbiology work. I have plenty of dehydrated media for both nutrient agar and trypticase soy broth. If I need to make some trypticase soy agar, without having to order new media, can I make the broth first and then dissolve the nutrient agar media in the broth?

I thought about HR and AI, in a way to study how AI can enhance HR practices?

However there are not much data about this, and i don´t know what to do in the case study part.

Videoconferencing is one of the Internet services that are currently developing intensively. The progress in the field of data teletransmission in the Internet allows for the gradual improvement of techniques enabling online Videoconferencing via the Internet.

In addition, completed Videoconferences after recording can be placed almost automatically on the profiles of social media portals.

In connection with the above, Videoconferencing is an excellent form of sharing experiences in the field of scientific research conducted by scientists.

It is also a good way to popularize scientific knowledge. In addition, debates may also be held on the Internet, and Internet-related questions may be asked by the speakers. Questions can be asked by listeners who watch Videoconferencing broadcast live, online through the Internet.

In view of the above, the current question is:

Please, answer, comments.

I invite you to the discussion.

Best wishes