Nanoparticle Synthesis - Science topic

I'm working on synthesizing 1T MoS₂ via a hydrothermal method similar to the process described in the paper "A nano interlayer spacing and rich defect 1T-MoS₂ as cathode for superior performance aqueous zinc ion batteries."

The synthesis process involved dissolving 1 mmol sodium molybdate, 2 mmol thioacetamide, and 0.14 mmol CTAB, followed by stirring and transferring the solution into a 50 mL PTFE autoclave reactor for 24 hours at 200°C. I obtained a yellow to orange solution and after drying it weighing around 0.1 g.

I was expecting a higher yield based on the stoichiometry, but the actual product weight is much lower. Is this typical for this type of synthesis, or could I be missing something in the reaction? Also, how can I proceed with characterization with such a small amount of product?

Any advice or insights would be greatly appreciated!

Thanks in advance!

We are synthesizing ciprofloxacin-loaded albumin nanoparticles. Albumuin nanoparticles is synthesized with proper size. Zeta potential and stability by desolvation method. The problem is precipitation of ciprofloxacin-loaded albumin nanoparticles when we add ciprofloxacin-HCl (about 10 mg/ml) whether during albumin nanoparticle synthesis or after it for encapsulation of ciprofloxacin into albumin nanoparticles. We guessed that it maybe because of decreasing the pH of reaction by ciprofloxacin-HCl but initial increasing the pH of ciprofloxacin-HCl even to 11 did not solve the problem.

What is your suggestion for preventing the precipitation of ciprofloxacin-loaded albumin nanoparticles?

Thank you in advance

I am getting extra peaks in my XRD graph of essential oil mediated zno nanoparticle which supposed to be of zinc hydroxy nitrates from the literature review. Would this sharp zinc hydroxy nitrate peak has any impact on the membrane stabilizing activity and antimicrobial activity.

Buenos días,

Me gustaría preguntar si alguno de ustedes ha realizado la síntesis de nanopartículas de hierro recubiertas con ácido oleico. En ese caso, me interesaría saber qué disolvente han utilizado para el lavado, ya que al utilizar tolueno he observado que las nanopartículas se oxidan. Además, al dejarlas secar al vacío, presentan una textura pegajosa similar a un chicle.

Hey all

what are the ways by which an encapsulated drug inside BSA may escape from its encapsulation.

We had synthesised curcumin encapsulated BSA by desolvation method followed by centrifugation at 12k rpm for 30 minutes and water-bath sonication for 5 minutes.

We had sent the sample for FE-SEM analysis and could find curcumin in the background .

Is this the curcumin that got leaked after encapsulation or is it that one which wasn't removed even after multiple washing steps and centrifugation?

How do to find this out?

Does sonication break encapsulation?

If so how to prevent the leakage of encapsulated curcumin?

An image of the same has been attached for your kind reference.

Thanks and best

Suppose, a new metal nanoparticle synthesis process is carried out in my lab. And then, by which physical or lab tests confirm its nanoparticle confirmation ?

I'm trying to produce silver nanoparticles using plant extract, but I didn't observe the expected peak in the UV-Vis spectrum between 380-420 nm, where silver nanoparticles typically appear. After centrifugation, I obtained pellets suspected to contain AgNPs. Based on the provided UV-Vis spectrum, can it be inferred that AgNPs have indeed formed? Where might the AgNP peak be located, and is it possible that it's shifted outside the usual range?

Additionally, both my extract and the silver nanoparticles have a pH of 4-5. I'm curious about how I can adjust the conditions to make them more alkaline and optimize my "green" synthesis.

I would greatly appreciate any insights or advice on these questions. Thank you in advance for your help.

1. Which form of plant extracts shows the greatest potential for green synthesis of silver nanoparticle:

- direct homogenization with deionized water followed by filtration or centrifugation, or

- initial maceration with ethanol to form a semi-solid macerate later dissolved to a certain concentration with deionized water and filtered? Or can both methods yield effective results?

2. Should I dissolve the AgNO₃ and plant extract in deionized water, or can distilled water or even ethanol be used in the synthesis procedure?

I would greatly appreciate any insights or advice on these questions. Thank you in advance for your help.

I used Hydrothermal synthesis method...to synthesize nanoscale

MoS2 . NH2OH.HCl was used as a reductant to turn MoO6

in the form of Na2MoO4 into Mo^+4, which was followed by adding

Na2S to conduct sulfidation reaction.so that I shoud prepare nanoscale MoS2

 after the addition of HCl but I didn't precipitate

Initially, I conducted maceration with a 70% ethanol solvent for 3x24 hours to obtain the thick extract.

For the AgNP synthesis, the thick plant extract needs to be dissolved using deionized water as a solvent. However, upon dissolution, a significant amount of precipitate forms. I sonicated it for 20 minutes to aid dissolution, yet there was still precipitate present. Subsequently, I filtered it, resulting in a clear extract solution.

However, the resulting clear solution is unstable, even after storing it for only a day in the refrigerator, as precipitate forms again despite initially being a clear, filtered solution.

Are there any suggestions regarding storage or procedures for preparing the extract? Is it okay to filter the extract? Are there any suggestions regarding which filter paper to use?

*I do not use water as a solvent during maceration to ensure obtaining a thick extract so it will not be hard to determine the final extract concentration.

I would greatly appreciate any insights or advice on these questions. Thank you in advance for your help.

Hey all

Suppose the yield of nanoparticle synthesized is 20mg. How much of this should be dissolved in appropriate solvent and how much of solvent should this be dissolved in for Zeta and DLS analysis.

A colleague of mine suggested to dissolve 1mg in 5mL of Milli Q water ?

What is the appropriate amount ?

Thanks and best

I need refractive index of silver nanoparticles for zeta potential and zeta sizer (edited) analysis. I would be grateful if you could suggest it

Using the microwave-assisted polyol method, I'm carrying out a project that uses folic acid as a precursor to synthesize carbon quantum dots. Is it possible to substitute DEG, usually used as the solvent, with ethylene glycol and get good results?

Hi here, I bought this product "https://avantilipids.com/product/840875" in powder form. My protocol for lipid nanoparticle synthesis uses a microfluidic system from PreciGenome. I need to dissolve the lipid in ethanol for nanoparticle synthesis. I tried ethanol and ethanol-chloroform-methanol (major-minor-minor portion); but it is not completely soluble. I would really appreciate it if you could please suggest a method to dissolve DOPA.

why not any one?

The band intensities in different regions of the spectrum for the test sample was analyzed and according to that, peaks were obtained at 3450.80, 1632.76, 1411.85, 1102.76 and 652.23 most of that bands belongs to OH groups. How I explain in my thesis involvement of banana peel extract for urea nanoparticle synthesis process by using above peaks?

I have some queries regarding dispersion of ZnO NPs by ultrasonication and also solvents used for dispersion, like DMSO, ethanol, etc.,

Upon synthesizing zinc oxide nanoparticles (ZnO NPs) through either green or chemical methods, the resultant particle sizes are typically in the nano-scale range (nm). However, the size of the synthesized ZnO NPs may undergo alterations during the dispersion process through ultrasonication, deviating from the originally obtained sizes. How can one claim the antimicribial activity of originally synthesized sizes?

The solvents used for the dispersion of ZnO nanoparticles may possess inherent antimicrobial activity, resulting in a synergistic effect when combined with the nanoparticles as opposed to their individual activities.

When we use different salts as a precursor for nanoparticle synthesis, is it necessary that they be from the same precursor? For example, nickel chloride and copper chloride are the same; can we use any one of the nitrate salts?

An brief description of how various temperatures affect the process would also be appreciated.

I found some issues trying to clean a micrometric channel made of PDMS. This channel is part of a chip which has been used as a droplet generator before and it's 50 micrometers wide and 25 micrometers deep. Now I use it for Nanoparticle synthesis. I tried using a syringe pump loaded with DI water and it leaves clots of NPs inside of channel, making the chip useless. Do you have any idea which material (Like HCl solution) I should use and how to use it (Ultrasound or pumping)?

Thanks

Hey all

I followed the below attached protocol for synthesis of Chitosan nanoparticle. However after 12 hours of continuous lyophilisation I didn't get it in the powdered form.

How does chitosan nanoparticle look to the naked eye? Do they look like a powder?

Good evening everyone

I am working on green synthesis of selenium nanoparticles from leaf extract. The research article mentions that during selenium nanoparticle synthesis, ascorbic acid should be used as a catalyst. so why there is a need to use a catalyst?., and what is the best drying method for synthesized selenium nanoparticles?

Does anyone know?

1. Do biosurfactants still have the capacity to reduce surface tension when used as nanoparticle stabilizing agents?

2. When nanoparticles are produced mediated by biosurfactants, are they in the form of liposomes or micelles? by considering several references using the Riverse microemulsion synthesis procedure (water to oil)

I want to study the opto-structural and magnetic property by XRD,XPS and SEM analysis.

I want to prepare a solution of TiCl4 in Millipore water for the Titanium nanoparticle synthesis.

Hi all,

I am currently working on a microfluidic system where two fluids are introduced with adjustable flow rates into a mixing chamber. The purpose of this system is to facilitate nanoparticle synthesis. The construction of the microfluidic part of my system is completed, and I have a working model of the mixing process that does not include the synthesis aspect.

My inquiry centers around whether anyone has expertise in modeling the synthesis that occurs within such a system. Specifically, I am interested in any type of synthesis modeling as my primary goal is to ascertain the feasibility of simulating this process. Additionally, I am curious to learn which physical principles I should take into account when developing my model.

Any guidance or references to relevant literature would be greatly appreciated.

Thank you in advance for your time and assistance.

I am working on Chitosan nanoparticle synthesis and characterization. But I can see the nanoparticle aggregate after some time and hence the respective increase in particle size. How to increase the stability, please suggest.

After nanoparticle synthesis using plant source, we need to take the absorbance data to see the high peak. My question is -

Do I need the blank to get the absorbance data or I can get the absorbance data by keeping the nanoparticle solution in different wavelength?

I would like to synthesize SnO2 nanoparticles from SnCl4. Having problem with ppt washing. Precipitates washing away during cleaning. Please help with expert opinion or suggestions.

I made HSA nanoparticles by desolvation method. I took 180mg HSA and dissovled it in 4mL of milliQ water (45mg/ml) and then in the protien solution added 2.5mL of ethanol dropwise, then these nanoparticles were stablized by adding EDC (5mg in 0.5ml of milliQ). The solution was left for 3hrs for nanoparticles synthesis under constant stirring at 1250. The nanoparticles size was checked by zeta sizer and the size found to be 64nm. Then i tried to settle down the nanoparticle at 13000rpm for 10 min but I'm not getting pellet. Further I also tried to increase the cetrifugation speed and time still i did not get the pellet. Any suggetion about this query would be appreciated.

Dear beloved scientist.

I have a question regarding ZIF-8 Nanoparticles synthesis. I used Zn(NO3)2.6 H2O as Zn2+ precursor and 1,2-dimethylimidazole (1,2-Dmim) as the Mim source with molar ratio of 1:4. Then they were solved in 50 ml methanol and stirred for 24 h at room temperature. But, after 2- 3 hours, there is no color change in the reaction solution. I use this journal as my reference for synthesis ( ). Is it normal to have no color change after several hours? How the color of the solution of the success reaction of ZIF-8 NPs? Because it's my first time to do this synthesis. Do you have any opinion that can improve my method? Your opinion regarding this matter is very welcomed.

Thank you.

What are the biggest technological challenges in the production of core-shell nanomaterials?

Can you please tell your experience and/or give comments on morphology control, synthesis precision, stability and durability, economic viability, etc.

Dear researchers,

Is there any method or device like Amicon 0.5ml ultracentrifuge (Its highest cut-off is 100kDa and not good for my purpose) that I could purify my small amount of volume nanoparticles that are conjugated with high MW polymer (more than 400kDa)? I tried 0.5ml ultracentrifugation of 135k*g for 1 hrs but the pellets are not obvious based on a small initial amount of lipid particles that contain nucleic acid.

I am currently working on chitosan nanoparticles synthesis by ionic gelation method. I dissolve chitosan in 1% acetic acid (to make a solution of 1mg/ml) Regarding that I have some queries:

I'm at the end of my preliminary experiment for the synthesis of Hap, but I noticed traces of black ink in my dried hydroxyapatite, I tried to search at which temperature the component in black ink decomposes, but I'm still quite unsure.

Best Regards

-Flynne

e.g: nickel nanoparticles by hydrazine

Explanation.

PVP behaves as a capping agent in the synthesis of Ag nanoparticles.

For Example, 1 At% / 1 Wt% / 1 mol% of Zn doped in TiO2.

In order to synthesize silver nanoparticles with specific shapes such as triangular or decahedron, we usually add capping agents such as PVP or citrate to the solution. for higher concentration solution the amount of these organic compounds should be increased inevitably. After drying one droplet of the final solution, thick layer of organic materials covers the nanoparticles (as shown in the attached figure).

I have seen that in many papers people suggest using "dialysis bag" or "centrifuge tube with filtration membrane" but both of these techniques may destroy unstable nanoparticles (specially triangular shaped N.Ps). Do you have any suggestion for getting rid of any organic materials form the final solution with out sacrificing the nanoparticles?

I synthesize mesoporous silica nanoparticles, then the glassware has a white residue in its walls, so how can I eliminate this residue?

Looking forward to the dynamics of plasma - electric field distribution.

and evaluating the forces in plasma - PCVD- nano particle distribution.

I used an initiator for polymerization of a monomer, is it possible to use the same initiator for the same monomer to synthesize polymer nanoparticles?

regards

Hello, I'm working on interactions of some inorganic metals/compounds with some protein of interest. Here the ligand are in nanoparticle form. So as for ligand preparation for docking these need to be in nanoscale range, is there any procedure for preparing it ?

Please suggest some user friendly software other than Vesta if possible!

Thanks in advance.

Regards,

Vinay

The fate of nanodrugs / nanoparticles in vivo draws a lot of attention, and many studies label fluorescent of nanodrugs / nanoparticles in order to disclose their distribution in vivo.

- What are its advantages and disadvantages ?

- Is it a reliable tool ?

Please treatment would you recommend for my mortar and pestle as well as the magnetic stirrer that got stained (with black) after using them for magnetic nanoparticles synthesis?

Thank you

I am currently working on Janus nanoparticles synthesis. I have been tried to wash the colloidosomes with chloroform several times by stirring and centrifugation, and even tried chloroform followed by recrystallization in cold methanol, unfortunately I could not obtain the Janus nanoparticles meanwhile the SEM pictures clearly show the nanoparticles onto the wax surface. Could someone providing me with any suggestions?

Kind regards,

Dear Everyone. I am trying to do the high yield synthesis from ascorbate and copper salt, according to the similar procedures from the literature (0.18M copper ions) I now tried PVA up to 2%, Tween 20 up to 10%, both, none, dropwise addition of ascorbate, burst addition of ascorbate, nitrogen, air atmosphere, temperatures 50, 60, 90 centigrade. I followed a couple of similar syntheses from the literature, nothing works. All the time instead of suspension I get a salmon-colored precipitate that for sure is not a dozen nanometer copper. What do I do wrong?

Thanks a lot for all the hints

In nanoparticles synthesis, reducing agents change metal into its zero oxidation state,which form cluster with other atoms and then coated with capping agent, why necessary to change metal into zero oxidation states, which force responsible to form clusters?

I am preparing nano ferrites by using microwave hydrothermal method to get the precipitation. We are adding NaOH with maintaining pH, I would like to ask what is the main role of pH?

Does it affect particle size of material? How?

Hello!

Ive never made micelles before, but I gave it a go today and now I have some questions. I am generating thin films using my polymer solubilized in chloroform, evaporating in a rotovap, and then resolubilizing in water followed by 30 min of sonication. I currently (very sadly) do not have access to DLS, I am thinking about getting the Lens3 from Tosoh and analyzing my particles using MALS very soon, until then I dont have any physical characterization assays setup. As a very general description of my molecule, I have conjugated a hydrophobic molecule onto a cationic/hydrophilic polymer

1. What size flask is appropriate for 2mgs of polymer? I tried 100mL, 250mL, and 1L. the 1L sized flask was a bit cumbersome to do the hydration in and the thin films from the 100 or 250 mL flask dont look that thin

2. How quickly do I add water to the solution? Dropwise or all at once?

3. Should i start sonicating the micelles as they rehydrate? Should I rehydrate and then wait and then sonicate?

4. Does adding salt to micelles during hydration or after hydration change particle size?

5. How stable are cationic micelles in general in water or buffer? Hours? Days Weeks? The paper im referencing has no stability data

6. Are there any solvents that are not acceptable for forming thin films/micelles? Variants of my polymer are soluble in ethanol but not in chloroform so can I use ethanol instead?

Im still going through all the literature to find answers to some of my more basic questions, but any useful papers you can recommend would be very much appreciated and a big time saver for me!

Which one is better?

At the first 1 g of HIPS polystyrene thermoplastic polymer was mixed with 10 cc of toluene solution for 1 hour and a half using a magnetic stirrer.

The resulting solution was mixed with 0.2 g of hydrophobic sio2 nanoparticles for 1 hour using a magnetic stirrer.

The solution obtained from the previous step was dispersed under ultrasonic bath ( 5 x 100 second cycles )

Then polydimethylsiloxane Silgar 184 was mixed in a ratio of 10: 1 with the solution obtained in the previous step for 30 minutes.

And then 500 seconds (5x100) again with ultrasonic.

The resulting solution was immersed twice as a coating on aluminum substrates, wood and glass slides.

After 24 hours, the coating was dried in the oven for 30 minutes at 100 degrees

The contact angle of the water drop was 118 degrees (without polydimethylsiloxane) if the contact angle with polydimethylsiloxane was expected to increase but decreased to 100 degrees.

Is there a mistake in the protocol?

Is there a problem with the time it takes to put it in the oven?

nanoparticles synthesis and applications

Stocks of KHP near me ran out due to pandemic and it would take months to arrive if I order overseas. Is it really necessary to standardize them in this type of research? If not, can you please share me an article about it? i would gladly accept your answers

Hello everyone,

I hope you are doing well in this pandemic.

Currently, I am doing PhD in powder metallurgy. I am in the stage of submission, so looking for a postdoc. But, I am a little bit confused about the selection of the topic. I have worked on metal matrix composites, polymer matrix composites, and nanoparticles synthesis during my M.Tech and PhD. I have published papers in all three areas. Can anyone suggest which topic will be better for me (in terms of future scope, novelty)? I am looking for your valuable suggestions.

Thank you

It is hard to find any reference related with gold nanoparticle synthesis using plant extracts that were extracted using ethanol solvent and has weight concentration (m/v) as one of its optimizing parameters aside from other parameters such as temperature, pH, HAuCl4 concentration (mM). I need the weight concentrations parameter for me to refer to and use in my current work for a research project in Masters's.

Hi community,

I am currently looking into strategies to reliably and robustly generate, preferably in the direction of minimum 50 mg total metal quantity per batch, colloidal bimetallic Janus NPs. While I have come across a variety of options lately (see the references at the end of the question description), there may be some expert advice out there that could point me at important considerations that I have previously not thought of (so I am more asking for directions, rather than a chewed out protocol).

I decided on a simple starting point and created spherical Au NPs around 10-20 nm in diameter as a seed (and reference) material for further ' bimetallization'. These were prepared by the established citrate-reduction method. Then I would like to perform a second step in which I grow the metal of lesser nobility on the Au seed surface. For a metal like Ag, that step seems rather simple as the metal precursor is added with additional reductant and stirred/refluxed for minutes to an hour to complete the process (e.g. [1] or [2]). In combination with Cu, there are also several reported methods (e.g. [3] or [4]), though these two in particular use inert atmosphere to effect the synthesis, which I think should not have to be a necessity to prepare AuCu NPs in general (and would make reproducibility labile I think). Metals such as Ni, Co, and even Fe can be made into a Janus NP with Au (e.g. [5] and [6]). However, I am avoiding a one-pot method to co-reduce the noble and non-noble metal concomitantly, as I would like to exert more control on i) NP size by first establishing the seed size, and ii) its growth mode on the seed by varying e.g. ligand/surfactant concentration (though this reasoning may not be entirely grounded for going with a two-step process).

Any suggestions would be helpful.

References:

[1]

[2]

[3]

[4]

[5]

[6]

I have been synthesizing albumin nanoparticles for about 5 months. But for the last 1 month, I have been having trouble with nanoparticle synthesis. I can precipitate nanoparticles in 1 of the 5-6 experiments I have done. Suspecting that the BSA was broken, we ordered a new one and the result did not change. What could be the problem?

hi I'm currently trying to synthesize nanoparticles SnO2 by co-precipitation method. From the XRD result, it appears that SnO2 have been detected. But when I tried to check the optical band gap with tauc plot method, the optical band gap was far below 3.6 eV (which is the bulk SnO2 optical band gap). Is there any explanation what causes this? (the absorption curve in the attachment)

In nanoparticles synthesis the use of hydroalcohol solvent in soxhlet extraction is correct or not... and I need article reference for this.

I have synthesized NiO nano particle. And they shown change in properties when we goes from bulk to nano regime. In bulk regime shown transition metal oxide but in nano regime shown semiconductor. Why this properties will be changed? I want to know the exact reason. Please explain it in details.

In one reaction, cyanamide and 1,6-diaminohexan were used to link one compound with the NH2 functional group and the other with the COOH. I do not understand exactly the role and function of cyanamide and 1,6-diaminohexan. Can anyone help me?

I tried to synthesize samarium nanoparticles using curcumin. I used samarium nitrate as a processor and curcumin as a reducing agent. I put the solution of samarium nitrate and curcumin on a magnetic stirrer at 80 ° C for 4 hours. But after centrifugation, I got a small amount of precipitate and could not dry it.

I also put samarium nitrate and curcumin solution in an incubator shaker overnight. No precipitate is formed after centrifugation.

Should I use another substance (eg samarium chloride or samarium oxide) instead of samarium nitrate?

volume ratio of Nickel acetate and Tannic acid?

I have heard, that larger the ligands, more better the core shell structures due to the packing of the which in turn results in a decrease in the density of ligands on the surface of the NP.

Is is true? How branching affects the formation of core shell?

I am trying to find the reduction potential of long chain primary alkylamines, in particulcar Dodecylamine and Oleylamine.

What it could be?

Hello,

During the synthesis of nanoparticles, many at times a particular pH value is optimum for that process.

I am confused as to whether the pH needs to be adjusted during the reaction while mixing the precursors, or after mixing the precursors (followed by continued stirring for a certain period of time).

Does it depend on the reaction or is there a preferred way?

I am asking this as I have found both of the above mentioned approaches in papers.

For example for the synthesis of covellite copper sulide (CuS) nanoparticles:

Thank you

They need to be agglomerate free. They can be in any aqueous or alcohol solution. These are commercially available, but the vendors generally don't tell you how it is done.

Please i still want more clarity on synthesis of silver nanoparticles. I tried different parameters like changing concentration of plant extract, for silver nitrate and so on. I observed color change from green to dark brown but yet there is nor SPR at 420. My peak is now at 345. Is it possible that my plant extract cannot reduce silver nitrate or what is the justification? Or maybe the problem could be silver nitrate?

I have encountered some studies doing this through some calculations involving FWHM values of PL bands but I couldn't figure that out. I am calculating radii by using the effective mass approximation. EMA predicts radii through bandgap energy, therefore, the output is unrealistically precise and doesn't have any error function. One of the reviewers especially asked for the size distribution calculation from the FWHM value of the PL band.