Questions related to Nano
X-ray crystallography with wavelength of order length -1nm, can't resolve a length order more than 1 nm. Any length order from XRD with wavelength order -1nm if any one is reporting then that must be manipulated one. I will be happy to answer if any one has any queries to the above statement. Moreover I will be happy to answer queries deal with characterization of Nano order length using Small Angle X-ray Scattering (SAXS) irrespective of any science i.e. physical, Chemical, Biological, Engineering, Medical & Natural.
The work function of bulk silver is cited as 4.6eV. Will there be any change in the work function of the metal when the dimension is reduced to nanometer? In this case a nanowire whose diameter is less than 100 nm and length is about 10 um. Will a electrode composed of the above mentioned silver nanowire have the same work function as that of bulk silver or will there be any change due to the nanoscale dimensional constraint?
hello i am writing a paper on comparing few nano fluids for active cooling of batteries. i have researched multiple times, but was unable to find thermo-physical properties of nano fluids to use them in cooling of batteries. can you please suggest me thermo-physical properties of nano fluids. here are few examples of nano fluids considered. water + Al2O3, water + Cu, water + CuO. what concentration of nano particles in nano fluids to use them for battery cooling ?
I am working on nanoparticles i need the size of Fe3O4 nanoparticles below 10nm I am using sodium citrate as a stabiliser. In one case I use NH4OH in solution the color of the solution turn black but in the cetrufuge, the nanoparticles are not collected. my teacher said if the color turn to black then nanoparticle absolutely formed find a way to sit down. the method i am using is co-precipetation.
I am a researcher in Microbiology. I am trying to prepare a nano material from hydroxy appetite. Can anyone give me a brief on how to do that?
I have synthesized the plant based-Fe and Ag nano particles. Can some tell me that how to depict the FTIR result of Nano particle and plant extract? As some peaks are coming in FTIR that is not reported earlier.
first time asking question, trying to use UV light to excite Sn and SnO2 and detect the fluorescence, however there is nothing be detected. help
I am trying to perform isothermal titration calorimetry (ITC) for quantifying DNA and liposome binding thermodynamics, but the samples are in low volume and very low concentration (~40 nM). Do you think a Nano ITC instrument (compared to a regular VP-ITC) would be able to be used for this low-concentration system?
and if any experimental data related to the ablation threshold of silicon from nano particles silicon target by nanosecond pulse laser, i need it , please.
I am conducting a MDCK cell permeability assay.Below is the protocol I followed :
1. In the apical region, 100 ul cell suspension containing about 33,000 cells were seeded in each insert in 24 well transwell plate (6.5mm transwell insert diameter, 0.33 cm2 insert membrane growth area and 3um pore size).
2. 0.6 ml of media (EMEM with L-glutamine) was added in the basolateral region.
3. Media was replaced each day in both apical and basolateral region.
4. On 7th day, Lucifer dye assay was conducted. 3 wells were used for lucifer assay. Briefly, inserts (appeal region) and basolateral region were washed with prewarmed HBSS. 100ul of 100ug/ml of Lucifer dye solution in HBSS was added in the apical region. 600ul of HBSS was added in the basolateral region. Samples from basolateral region were collected after an hour and checked for the fluorescence.
Results showed no permeation of lucifer dye after an hour.
5. Now, for permeability assay, first, the inserts were washed with HBSS and then added 100 ul HBSS in apical and 600 ul in basolateral region and kept for 30 min in the incubator for equilibration.
6. 100 ul of nanoformulation and free drug control formulation (containing 100ug drug) was then added in each inserts and kept on the belly dancer (slow shaker to avoid the stagnant layer).
7. 200 ul Samples were then taken and replaced with fresh HBSS at 30 min, 1 hr, 2h, 3h, 4h and 24 hour.
8. All the samples were analyzed using LCMS.
9. Results showed no permeation at 30 min, 1 hr, 2h, 3h and 4h from both nano formulation and control formulation. However, it showed permeation at 24 hr with 2.23% from nano formulation and 1.22% from control.
10. Results also showed, about 3% drug was left in the apical region. Meaning, about 93% drug is stuck (or absorbed) in the cells.
Could anybody suggest if I am missing any step in the protocol?
Thank you so much.
We were surprised during the preparation of magnetite (Fe3O4) usung the co precipitation method
During the preparation, we started as usual with 1:2 ratio of Iron II and iron III
salts , we added the ammonia under N2 and everything is perfect
When we used ethylene diamine to functiolize the surface, things went OK and we got the beautiful black Fe3O4 NPs . But when we used phenyl diamine, it did not work and the stuff was oxidized to brown Fe2O3!
I have done 285 samples of human blood DNA extraction, and measured the DNA Concentration and A260/280 and A260/230 in a nano drop machine. This is done before PCR. Now the values of A260/230 are less than 1.5, most of them are 0. or 1. something.
Now After doing pcr and gel run, I want to do sanger sequencing, wil there arise any problem due to the purity value of A260/230???
I have capped ironoxide nano particles with hydrophillic amino acids which show good stabilisation ( no sedimentation ) in neutra pH. How ever when the Zeta potential was measured, the value is ~ -8mV. As per my understanding the stable supensions have zeta potential value of |30| mV or higher. would anyone please help on this regard. Thank you in advance.
In "Parameters effecting on photocatalytic degradation of the phenol from aqueous solutions in the presence of ZnO nano catalyst under irradiation of UV-C light"
1) Why when the nanoparticles increase more than 0.15g/l, cause decrease in kinetic reaction? and how we can solve this problem?
2) why increase of PH, the phenol degradation decrease? and how I can solve this problem?
I need to quantify the immerging micro & nano plastic particles from wastewater sample, so can anyone tell me which techniques shoild be followed for beter result regarding this case?
I' am researching on nano technology recent days, especially in use of nano composites in sensors and super capacitors. So I need to know about the software used for the simulation of these kind of things.
As in fungal nail infection the PH of nail increase from 5.5 up to 6.4 in acute and above in chronic nail infection. so, why we perform release and permeation study at 5.5 rather than at higher PH to confirm that the selected nano formulation show efficient release and permeation at infected nail PH or not?
How nano fertilizers a sustainable technology for improving crop nutrition and food security and role of nano fertilizers in agriculture?
Hi there RG forum,
Does anyone knows about a reliable (and already conjugated) tdTomato-nanobooster for clearing experiments?
There are plenty of GFP boosters out there, but is hard to find tdTomato boosters. Moreover, the RFP-boosters seem to not detect tdTomato...
Any suggestion will be more than welcome,
Thanks a lot,
Dear ResearchGate members,
I would like to know if polymers such as Polyvinyl alcohol, Polyvinyl pyrrolidone, Chitosan, Carboxymethyl cellulose, etc. (with a molecular weight less than 40 kDa) have a lambda max in UV-Vis spectroscopy. If they do, how do they not interfere with drug estimation in nanoformulations/nanoparticles (polymer-based nano)?
Thanks in advance.
During a green synthesis of Copper oxide nanoparticles using a given plant extract, Nano Copper hydroxide sulfate and nano copper oxide were formed . the xrd showed the presence of both( cuo and the hydroxide sulfate)
First i used NaOH to manipulate pH. Did the Nano copper hydroxide sulfate was formed due to the presence of NaOH or as a result of the used plant extract?
Second in the xrd, there are common peaks between both compounds. how can i realize if these peaks belongs to a given compound rather than the other?
I was to that chitosan nano particles cannot be used as a carrier for soil bacteria due to their antimicrobial properties. Its it possible to use chitosan
NP in its crude form or is there a way to modify it to be usable as a microbial carrier?
I,m synthesis carboxy cellulose nano fiber but gradually material's pH going to acidic without centrifuge. now my step is drying this material's. how can i do this?
I have to take absorbance of nano particle solution. For this which solvent is recommended to use as a blank? Is it only silver nitrate solution or anything else?
I have gone through several literature where all of them prepare powder from nano particle solution after preparation of nanoparticles solution using plant extract. If we think about the application of nanoparticles, Why we create powder rather using the liquid solution (Aqueous solution can be considered) ?
I used untreated cotton fabric,treated fabric with silk and silver mixture,silver nano particle, silk nano particle
Here, s4b(1,2,3) are untreated cotton fabric,s3b(1,2,3) are treated fabric with silk and silver mixture,s(21,22,23) are silver nanoparticle and s(11,12,13) are silk nanoparticle
Please anyone interpret the SEM analysis result
I used the hydrothermal method to create nanoparticles and adjusted the time and reaction temperature to produce catalysts of different sizes. To validate the change in surface area, I conducted a BET analysis and found a significant change in surface area and pore volume. It would be beneficial if I could also demonstrate the variation in electrochemical studies. I performed fundamental tests such as CV, LSV, and EIS. However, to measure the specific area, I aim to determine the electrochemically active surface area.
Please help me with this...
Is micro-strain (ε= β/4tan θ) and Dislocation Density (δ=1/D^2) applicable to natural fiber urea formaldehyde composites as well as inoc,nano and metallic materials? if yes, then can anyone share research paper on it's my findings of those parameters are δ=3.708807 and ε=4.384302.
I produced chitosan nanoparticle nano fibers and the density of pure nano fiber solution was higher than drug loaded solution.
- What is the suitable solvent to dissolve( nano α-Fe2O3 (hematite) in antibacterial tests? I used DMSO, but the nano particles were not well dissolved in it and were not homogeneous.
- Is there a way to homogenize hematite in DMSO?
How the xrd values effect the medium , bulk and nano powders in the zinc oxide .
I had an Mg(OH)2 (nano) sample where I had an XRD pattern which looks just fine.
The same sample was taken to undergo a TEM analysis along with SAED. The SAED pattern were missing the (101) line despite being the major line in the XRD pattern.
light is constant speed , how we can use a certain value 3 × 10⁸ m/s for c in equation E = mc² . value of speed of light change in different units of time , nano second , micro second, minute and hour . if I use light speed relative to a hour it will be 1.08 × 10 ¹² m /s .
And this number will change the result of equation E = m c² . but in all books I have seen speed of light value is used in per second term . why ?
I‘ve designed a peculiar geometry of resonator laser in FDTD and placed a nano particle in vicinity of that subsequently calculated the optical forces on this particle by MST analyzer eventually found the optical forces enormous in order micro or more . how to refine my work to achieve the correct amount of calculated optical force on particle exclusively in order pico or femto newton?!
What are the latest advancements in nanotechnology for solar cell manufacturing, and how does the use of nanotechnology contribute to increasing the efficiency of solar cells?
I have centrifrigmfuged the nano suspension at 10000 rpm for 3 times but after lyophilization i can not find free flowing powder
a. +4 is the place of co2 molecule in front of the graphene.
I need the standard XRD analysis data of Graphene Oxide. I do not need the graph as I have it. I need the data table (Intensity/2theta) for comparison purposes in Origin.
I have synthesized a new material using GO that I purchased from US Nano. Unfortunately, however, I do not have access to my GO anymore.
Sincere thanks for your help in advance.
What are the effects of nanofertilizers on plant growth and development and their interrelationship with the environment and effect of nano technology in agriculture?
we have NCS3500RS nano pump (Ultimate3000 Dionex Thermo) and after a couple of years of use it started to sporadically show an error "Illegal left WP home position. Please execute selftest.". The error seems to be critical for the system since it stops the analysis. Does anyone know the meaning of the error and how to solve it?
I need to coat a thin film of commercially available BaTiO3 nano particles on a substrate with a good adhesive property! Need to use this film as a piezo film to generate power!
Please suggest any solvent or polymer to make this possible?
The two terminology is confusing. Does in nanoconjugate curcumin is also nano sized and then used as reducing and capping agent to prduces Ag Nps? or it is same as curcumin stablized/capped/modified silver Nps in which curcumin is used as reducing and capping agent both?Does curcumin is present in nano form? or not? please clear my query as this is very confusing.
What is the best method to check the releasing of Antimicrobial drugs from a nanocapsule and encapsulated efficiency of capsule for that Antimicrobial agent?
Please, how can I sterilize a nano preparation if it is affected by light, and it is in colloidal state and may lock the syringe filter? Later I have to study its effect on cultured cells.
Thanks in advance
What’s role of nanotechnology in crop improvement& how much percent crop field can be increased by using nano fertilizers?
What is the use of nano fertilizers to improve the nutrient use efficiencies and Nanofertilizers towards sustainable agriculture and environment?
How nanofertilizer& nanopesticides are applied for improvements in crop production & protection & effect of nano fertilizers on crop sustainability?