Science topic

Mosquitoes - Science topic

Insecta: Pterygota: Neoptera: Diptera: Nematocera: Culicidae
Questions related to Mosquitoes
  • asked a question related to Mosquitoes
Question
3 answers
I'm currently investigating the larvicidal efficacy of plant extracts and some pure isolated compounds. Due to limited amount of materials, I may need to use lower volume of water compared to the WHO guidelines (100 - 200 mL). I've seen research papers using 6- and 24-well plates with 1 mL of water per well. Is there a huge difference to the outcomes between 1 mL and 100 mL of water being used? Thank you in advance!
Relevant answer
Answer
Yes he does
  • asked a question related to Mosquitoes
Question
5 answers
Hi everyone,
Who can tell me is there a link between the knock down time 50 (KdT50) and the resistance mechanism of the mosquito? in other words can we say that if the knock down time is high then the resistance is linked to the kdr gene ?
Relevant answer
Answer
Yes Souhail there s a clear correlation between kdr frequency and KDT (see papers of Chandre et al 1999 2000). It is even better correlate to KD than mortality so it s why we called it KD resistance.
  • asked a question related to Mosquitoes
Question
2 answers
Which variables can be successfully manipulated to get the F1 wild caught mosquitoes to feed.
Relevant answer
Answer
blood should warmer mosquito should not have taken other meal for a day or so and room should be darker lights should be off and provide dawn dusk mimic in insectory to make them function properly
  • asked a question related to Mosquitoes
Question
4 answers
I need to add taxonomic related vouchers for both adult male and adult female tea mosquito bugs in my article. Hence guide me to search for the same.
Relevant answer
Answer
Probably, you need publications containing descriptions or keys for determining species of Helopeltis. For example:
Stonedahl G.M. 1991. The oriental species of Helopeltis (Heteroptera: Miridae): a review of economic literature and guide to identification. // Bulletin of Entomological Research, vol. 81: 163-190.
Nobody calls such literature "vouchers", because different researchers can have different opinions about status and characteristics of species.
  • asked a question related to Mosquitoes
Question
2 answers
I am looking for mosquito population data for Mauritius. Can someone please help me? Thanks in advance.
Relevant answer
Answer
Contact the Department of Health in Mauritius, they have a vector control officer.
  • asked a question related to Mosquitoes
Question
4 answers
How to control bacterial scum from mosquito rearing trays with out affecting them ?
Relevant answer
Answer
A few ideas for you. Try to use fish food low in fat content. Oxygenate the water as per fish aquariums. Run paper towelling across the surface of the water daily. Reduce the amount of food. Replace some of the water daily.
  • asked a question related to Mosquitoes
Question
2 answers
Can anyone recommend a vendor that sells mosquito salivary recombinant proteins Aed a 1, Aed 2, Aed 3, or Aed a 4, for use in epitope mapping and other immunological assays?
Relevant answer
Answer
Thank you very much for the link!
  • asked a question related to Mosquitoes
Question
3 answers
I am doing whole cell patch clamp recordings on mosquito brain cells where I can form gigaseal but after breaking in into the cell there is gigaseal formation again even if I try to break in using suction or zap and the resistance value is usually on the higher side and even if it drops after applying suction the cells are lost then.
Relevant answer
Answer
In my experience (albeit with mammalian cells) some likely causes might include
-sub-optimal air table vibration dampening
-electrode drift (this can cause a 'taffy pull' on the cell membrane- creating a narrow high resistance cytoplasmic bridge from the electrode tip to the cell). Sometimes blocking or redirecting airflow from nearby vents can help, triple-tape all lines (gas lines, perfusion lines, grounding wires, headstage cables, etc.) to the scope (tape once to the air table surface, and once again to the scope, and once again to the scope stage) with enough 'flex' between taping points to allow some movement
-osmotic pressure- we generally keep our pipette (intracellular) solution at 290 mOsm and our bath solution at 320 mOsm, but the actual values may be different in your tissue preparation
-ATP regenerating component of intracellular pipette solution has gone bad, remake new.
  • asked a question related to Mosquitoes
Question
2 answers
I am currently pursuing my research on the dengue-causing mosquito at its various stages. I'm studying the effect of microbial secondary metabolite-based nanoparticles against the vector that carries dengue. In order to extend my study, I would like to use the DENV serotypes to validate the cytotoxicity of the particles that I developed.
Relevant answer
Answer
I am not sure, but you may ask for this to ICMR-NIV Pune, with the permission you may get your desired lines, and pursue your work.
  • asked a question related to Mosquitoes
Question
4 answers
Is it possible to share data on mosquito species diversity among countries with land boundaries? The expected result is a revision to the mosquito species checklist between corresponding countries. The idea is that mosquito distribution does not respect the boundaries of different countries. They only respect the suitability of the environment they live in. The diversity data acquisition is based on a study of ecosystems and land use between countries. The endemicity status of each species is also becoming an important consideration. What needs to be done if the species data acquisition is possible?
Relevant answer
Answer
The Biodiversity Piracy Act most probably restricts such issues
  • asked a question related to Mosquitoes
Question
13 answers
Dear Scientists (Vector Biologist/ Public Health Entomologist),
Please look into the attached pictures and suggest what could be the reason for such type of mosquito larval mortality where they were covered in a slimy layer and died.
Also kindly suggest if any of you faced same problem and found the solution to avoid this.
Relevant answer
Answer
Hi,
We sometimes have this type of larval mortality with Ae. albopictus. This can be associated with bacterial growth or densovirus. In order to limit this, I advise you to change the water regularly (every days or two days), remove the dead larvae as soon as possible, avoid dense populations in a single tank, ensure that the room has a constant temperature around 25 : 27 degrees, and finally leave the water aside for a few days before transferring your larvae. You can also insert the food with a pasteur pipette at the bottom of the tank to limit the food on the surface.
I hope you find these tips useful
  • asked a question related to Mosquitoes
Question
6 answers
I have acess to some rare field samples, that have been stored in absolute ethanol. I know RNA can be extracted from such samples, provided the storage temperature was low (e.g., ). Does anyone have experience with small RNA isolation, including miRNA? And which method/kit seems to be approriate? Thanks!!
Relevant answer
Answer
Hi Lucienne. I'd be tempted to go with a total RNA prep using Trizol, rather than a specific microRNA isolation kit. That would be the best way of maintaining total yield.
  • asked a question related to Mosquitoes
Question
4 answers
Semi-polar limonoids wanted to be incorporated in O/W nano-emulsion for mosquito larvicidal application. The compound is poorly soluble in the carrier oil, but readily soluble in organic solvents ie ethyl acetate, acetone. The emulsifier I'm using is Termul1284. I have tried mixing the compounds with the emulsifier first, before adding in the oil and water. But I'm having doubts whether this would entrap the compounds in the carrier oil. Any advices? Thank you in advance
Relevant answer
Answer
I would suggest you to go for multiple emulsion-W/O/W or O/W/O.
then you can entrap the drug efficiently following the addition of a combined emulsifier( you can choose two surfactants) compatible with the drug.
The process of emulsification also helps in proper entrapment of drug.
Check for the aqueous phase, if any ions might hinder the process.
  • asked a question related to Mosquitoes
Question
13 answers
Especially in favor of females in this case.
Relevant answer
  • asked a question related to Mosquitoes
Question
9 answers
I have tried it by means of membrane feeding method (see the link), but could not get results.
Relevant answer
  • asked a question related to Mosquitoes
Question
2 answers
I want to assess the infection status of Aedine mosquitoes with regard to dengue virus in Zambia. Very little is known about the prevalence of the disease in the country.
Relevant answer
  • asked a question related to Mosquitoes
Question
3 answers
A new microbe was found last year to have the ability to block plasmodium transmission without hurting the mosquitoes, I decided to present a seminar on this... hence my need for a case study/studies to prove my point.
Relevant answer
Hi Precious,
Of Corse, there are many studies on the related subject. Here by some papers dealing on your request.
You can get more studies about the meaning subject in google scholar. I hope this suggestion is useful..
Good luck and success
  • asked a question related to Mosquitoes
Question
11 answers
Is there any study or evidence that highlighted COVID19 transmission through mosquito bite?
Relevant answer
Es un error la Covid 19 no se adquiere por la picadura de un mosquito.
  • asked a question related to Mosquitoes
Question
6 answers
Let say the mosquitoes were collected from different houses for sever days and then want to determine an average density of harvested local mosquito population.
Relevant answer
Dear Dawit,
Densities of mosquitoes collected using light traps can be expressed number of mosquitoes per trap/night. One can express the densities of total mosquitoes or species after identification. While counting the mosquitoes in a light trap collection, remove males and take count of only the female mosquitoes. For example, you collected 2000 females anopheles mosquitoes in 10 trap collections (it could be 10 traps placed simultaneously in one night or 1 trap placed daily for 10 nights) then the density will be expressed as 200 anophelines/trap/night.
  • asked a question related to Mosquitoes
Question
7 answers
On three separate occasions I have encountered this mite, what seems to be the of same species, attached to the proboscis of Aedes aegypti and Culex erraticus species of mosquitoes. My guess is that it is hitching a ride from something (flower/bird), but I haven't been able to find a mite that matches. I'm hoping an expert here can at least get me to a genus or know what host it is from. Thank you in advance!
Update (4/25/22) - The genus has been determined to most likely be Cheyletiella due to the rounded body shape and large palpal claws but the species is still unknown. Since this post, a couple more mites were found on Culex mosquitoes attached in the same manner (high up on the mosquito's proboscis). Two PCR attempts failed so it is looking like visual ID of the solenidion will be the way to go. As we prepare to begin mosquito trapping again, I am sure more specimens will be obtained for either stacked photography or SEM. Regardless of species, it is looking like this is a phoretic relationship. I have reached out to other mosquito biologists to keep an eye out for these mites while identifying mosquitoes under the microscope.
Relevant answer
Answer
Hello Beth
I agree with Owen being a member of Ornithocheyletia. Try to make good slides and run the attached key published by the wonderful acarologist Alex FAIN. Despite his revision of the genus was back in 1981, but it's really a comprehensive useful one.
All the best
Mohamed NEGM
  • asked a question related to Mosquitoes
Question
9 answers
We need the culture of three mosquito species namely Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus for our insectary establishment. We will be very grateful, if anybody inform us, from where we can get the same.
Thank You
Relevant answer
  • asked a question related to Mosquitoes
Question
3 answers
I am considering the use of CO1 to differentiate between different mosquito populations. Is it an ideal target gene? And does it have better resolution than ND4 and ITS1, ITS2 to differentiate between closely related species?
Relevant answer
Answer
No,
in the case of Culex quinquefasciatus and culex pipiens, we will get the same sequence ... better to correlate with morphology. ITS2 is a better option.
Please go through the following article
Anoopkumar, A. N., Puthur, S., Rebello, S., & Aneesh, E. M. (2019). Molecular characterization of Aedes, Culex, Anopheles, and Armigeres vector mosquitoes inferred by mitochondrial cytochrome oxidase I gene sequence analysis. Biologia, 1-14. DOI : 10.2478/s11756-019-00231-0. Publisher: Springer
  • asked a question related to Mosquitoes
Question
3 answers
If I understand correctly, the Sella score (based on Detinova, 1962) assigns unfed female mosquitoes to 1, and gravid to 7. This is in order or the digestion process, with 2 being freshly full-fed.
My question is, what would the Sella score be for a partial fed mosquito be?
I have many individuals that have only taken a little bit of blood (often collected by light traps), but the Sella score has 1 being unfed and 2 being fully fed.
Thank you in advance for any advice, and any references would also be very helpful.
Relevant answer
Answer
Yes, stage 3 and 4 as stages where there is only partial blood within the abdomen as a result of digestion
  • asked a question related to Mosquitoes
Question
3 answers
I wonder about the biology of mosquitoes in relation to sex, we can differentiate An. gambiae from An. funestus or Culex pipiens from the larval stage but can we also differentiate their sex during these stages?
Relevant answer
Answer
There is a difference in the size of males and females in pupal stage and in pupal stage males and females can be separated to some extent. However, I am afraid that the same can not be achieved in larval stage
  • asked a question related to Mosquitoes
Question
1 answer
I am preparing slides for the genitalia of male mosquitoes of the Culex pipiens complex. I read it is used cellosolve to dehydrate the genitalia. Any alternative to replace it?
Relevant answer
Answer
you can use Entellan
  • asked a question related to Mosquitoes
Question
7 answers
I need to hatch a lot simultaneously and they're being very stubborn despite multiple immersions in water.
Thanks
Relevant answer
Answer
I don't used. But I think percentage of sodium hypochlorite should be very low.
  • asked a question related to Mosquitoes
Question
3 answers
We are trying to isolate DNA from mosquitoes using chelex and saponin to no available. Anyone with an in-house protocol that works?
We will be grateful.
Relevant answer
Answer
Hi Dr. Shey,
From our experience and after trying several DNA extraction protocols for our mosquito works we started skipping this DNA extraction step and use part of the whole mosquito into the PCR mx and homogenise using steriled pistol and run in the machine and it works every time. You might give it a try and see .
Good luck!
  • asked a question related to Mosquitoes
Question
1 answer
I am trying to extract RNA from Aedes aegytpi to analyze gene expression in genes associated with the detoxification of insecticides. I am using the SV Total RNA Isolation System from Promega, but I got a very low yield of RNA (from 30 - 50 ng/ul). Can you share any tips or protocols or how have you solve low yield in RNA isolation from mosquitoes. Thank you very much.
Relevant answer
Answer
For RNA extraction you can use, Applied Biosystems® Arcturus® PicoPure® RNA isolation kit (Arcturus, biological Biosystems, USA), you can remove the DNA remnants with the Baseline-ZERO ™ DNase removal kit (Epicenter, Illumina) and the ribosomal RNA with the Ribo-Zero ™ rRNA removal kit (Human / Mouse / Rat) (Epicenter, Illumina) these protocols provide high RIN
  • asked a question related to Mosquitoes
Question
10 answers
Hello To have solutions of water with variable rate of PH, what is the solution (acid) to be metered, provided that this acid is not toxic by what one is putting insects in the water used. cordially
Relevant answer
Answer
Jiji p v, thanks for your comment
  • asked a question related to Mosquitoes
Question
4 answers
Hello,
Our lab's Mosquito culture has been overused and as a result we are a bit short on the numbers within our culture. Does anyone know where I can buy some Aedes egypti eggs / larvae online that ship to the UK?
Relevant answer
Answer
Please try ATCC
  • asked a question related to Mosquitoes
Question
4 answers
Hi everybody,
can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular protocol?
Thanks a lot in advance
Niccolò
Relevant answer
Answer
I agree with the dear J. C. Tarafdar
NucleoSpin Bead Insect DNA Isolation Reagent Kit
  • asked a question related to Mosquitoes
Question
2 answers
Hi all
I need a method to isolate mosquito cell membrane protein from whole mosquito abdomen. Therefore, I will need to remove the mosquito hemolymph and exoskeleton.
I have not find a clear published method to do that. Any suggestions would be super helpful
Many thanks in advance
Relevant answer
Answer
Hi, there is no way to remove the tegument chemically; you need to dissect and open the abdomen, in order to recover the internal organs and tissues. You can simply cut the tegument along the abdomen, and given that you do not need to conserve the integrity of internal organs, you can scrap the interior with a needle. If you are not habituated to dissecting insects, you would probably need to practice a little bit before having a proper result, but it is not difficult. I recommend using a micro-scalpel made of pieces of a breakable razor blade and Ringer solution for avoiding the tissue to get dry.
Best,
Claudio
  • asked a question related to Mosquitoes
Question
24 answers
Are you aware of research-proven natural remedies that can be used to prevent mosquitoes bites (repellent), either by local application or consuming more amount of such food?
What about after the insect bite: any herbal remedies that is research-proven to be effective in reducing the symptoms?
Relevant answer
Answer
Cajuput Oil that belongs to Myrtaceae
  • asked a question related to Mosquitoes
Question
14 answers
Hello Researchers. I am conducting a study on the determination of WNV on mosquito samples. I run nested PCR. Bands were shown to almost all samples during carrying out gel electrophoresis. After I sent the PCR product for sequencing service for 5 random samples, and the sequencing result for 4 out of 5 appears to be 100% percentage identification of my targeted virus (WNV). While one sample shows 98% as the highest percentage identification. Hence, I would like to know whether my results are true positive (valid) or false positive? May I know how to determine the false positive result (any criteria or book/journal reference) as my future reference?
My targeted primer is between capsid and pre-membrane.
Thank you for your thought and suggestion.
Relevant answer
Answer
Hi Natasha,
Hello, it is not the sequencing which will indicate if they are true or false positives but the controls that you should imperatively introduce ; PCR without RT, RT-PCR without RNA samples (just with the reagents)). You will need a lab dedicated to the preparation of oligo, reagents ... a lab dedicated to the extraction of RNA / DNA from samples / a place for RT / never use the same pipettes for loading PCRs on gels and starting the RT-PCR or DNA/RNA extraction.... There is several ways to avoid contamination from PCR products (use of dUTP and uracil-DNA N-glycosylase...) taht will diminish the contamination.
  • asked a question related to Mosquitoes
Question
4 answers
Medical entomology
Relevant answer
Answer
Mosquitoes biting activities vary from species to species. Nost start at dusk. Biting rhythm depends upon upon temperature and also on humidity. Primary malaria vector of the Subcontinent - An. culicifacies become active after mid night -maximum , An, stephensi starts at dusk. for detail see my papers in ResearchGate, particularly Bionomic of Mosqiuitoes of Changa Manga National Forest
  • asked a question related to Mosquitoes
Question
13 answers
If i have only blood fed mosquitoes and i have to perform WHO kit bioassay, can i do so? or only non blood fed mosquitoes should be used?
Relevant answer
Answer
The ideal WHO assay will be on unfed mosquitoes, if not available in the field then lba reared female can be used, In field collected mosquitoes separate different gonotropjic cycle so that you may also find out different rate of susceptibility. Good luck
  • asked a question related to Mosquitoes
Question
6 answers
I need the mosquito eggs to be hatched within a short period of time, but the eggs are taking upto 20 days. What should I do? Please don’t avoid this question.
Relevant answer
Answer
Dear Md. Forhad Hossain , take a look below article. It might be helpful for you.
  • asked a question related to Mosquitoes
Question
1 answer
Hi could anyone share their practical experience about Arboviruses detection from field caught mosquitoes. I have used mosquito samples to perform metagenomic sequencing and passaged on C6/36 cells for Arboviruses detection. I have detected a considerable number of reads for JEV/GETV and the similar samples produced CPE’s on C6/36 cells and also confirmed the particular virus presence by RT-PCR from CPE’s  positve C6/36 cells (Japanese encephalitis virus, GETV etc). However when I passaged the same samples on BHK cells, it don’t produce CPE’s.
Thank you in advance.
Relevant answer
Answer
In the lab always keep mosquitoes at 4 degrees C. Homogenize the mosquitoes (pool mosquitoes in groups of 25-50 mosquitoes/ group). Centrifuge each group at 3000 rpm for 10 minutes and use the supernatant in rt-PCR to detect the virus.
  • asked a question related to Mosquitoes
Question
8 answers
I am trying to connect with researchers in the UK involved in the area of mosquito research. Please connect or PM me if you are.
Many thanks
Mebs
Relevant answer
I will work on mosquito borne disease (Malaria) as Phd project, but not in UK , and you are welcome if you want to support me, also others are very welcome , and every one can be involved in our research related papers.
contact me if interested.
  • asked a question related to Mosquitoes
Question
4 answers
Hello Everyone,
I am working on mosquito genomics and trying to find a best protocol or method for RNA extraction from a single head and thorax part of an individual mosquito. The RNA will be used for NGS applications. Please suggest.
Relevant answer
Answer
Dear Walid and Shahid, Thank you very much for your documents.
  • asked a question related to Mosquitoes
Question
6 answers
I downloaded the yeast- CO2 mosquito attractants paper pub. Sept 2017 and found it has a number of typographic errors. The Kline publication (2007) is cited 2 times as #14 and #24. It would be good to fix this and a number of other errors. I cannot figure out how to email Dr. Aziz Muhammed directly through RG or I would do so.
Relevant answer
Answer
You've written the author -that's prob enough. Errata typically come from the original authors. Couple of typos and a repeated reference will not justify a publication of your letter.
What (sloppy) journal is this?
  • asked a question related to Mosquitoes
Question
3 answers
Context
Consider a simplified version of the ODE model presented in Marini et al. 2017 (see section Model calibration of Additional file 1 in that paper).
In this model there are two populations of mosquitoes, that go through their different life stages: egg -> larva -> pupae -> female adult. For simplicity the pupae stage is omitted in this example without loss of information (see attached file 1). When evaluating this system in Wolfram Mathematica and obtaining the Jacobian we arrive at the steady states (see attached file 2).
Problem
If we focus on the scenario where all populations are present (i.e. Ec > 0, Ea > 0, Lc > 0, La > 0, Ac > 0, Aa > 0) and substitute the parameters with any values at this steady state, the populations of larvae, Lc and La, exceed their carrying capacities, Kc and Ka respectively.
Question
Why is this happening?
Can populations exceed the carrying capacity if they are only limited by it on a single life stage?
Or is there a problem in the implementation in Wolfram Mathematica?
Relevant answer
Answer
Human overpopulation occurs when the ecological footprint of the human population in a given geographical location exceeds the carrying capacity of the place occupied by that group. From a long-term perspective, overpopulation can be further viewed as existing when population cannot be sustained given the rapid depletion of non-renewable resources or in light of the deterioration of the environment's ability to provide support to the population.
  • asked a question related to Mosquitoes
Question
13 answers
Malaria caused by Malaria parasites (Plasmodium species) is transmitted by Mosquitoes. Malaria especially in the sub-Saharan African region remains a major public health due to associate high mortality and morbidity particularly in the under-five.
Mosquitoes repellents are a major tool for the control of spread of the disease.
What is your take on the above question?
Relevant answer
Answer
Dear Prof. Alegbeleye, in Venezuela since there is a hyperinflation, and we don't have money to buy repellers, I personally have started to use several herbs.
One of them is called locally "ruda", another one are eucalipto leaves. Both have a strong smell that repels mosquitoes.
We have now a zika epidemic again, I guess, and our legs and arms are bitten all time by Dengue, Zika and Malaria mosquitoes (white legs) in most cities and fields. SARS-CoV-20 quarantine makes it worse.
But there aren't statistics provided by health authorities, hereby is hard to prove it, otherwise, my legs and arms are bitten, and I know what I have.
Sometimes these herbs work as repellents, it depends on the local weather. If it becomes warmer is hard to stop the mosquitoes, if it is colder, the herbs help. That is what I observe now.
  • asked a question related to Mosquitoes
Question
3 answers
Hello everybody, I am not a specialist and I want test the effect of some substances on mosquitoes and flies but I have no idea how can I get them and how to keep them alive for treatment?
Thanks in advance
Relevant answer
Answer
It also depends on the mosquitoe's type. All these could actually be sorted out if you contact a medical entomologist or anyone working on mosquitoes.
  • asked a question related to Mosquitoes
Question
6 answers
I want to measure mosquito body weight post emergence and would like to find out if I can just take off the wings and weigh the body without drying the mosquito.
Relevant answer
Answer
It depends on the objective of your study, for example if it's for simple identification you won't need to dry the mosquito, but if it's for molecular biology tests you will need to dry the mosquito with suitable products such as silica gel.
  • asked a question related to Mosquitoes
Question
4 answers
Is there any study published and demonstrated which indicates the presence of the agent of rickettsioses? in other arthropods not hematophage.
Relevant answer
Answer
Thanks a lot, but I was wandering.
Why we find the rickettsies only in the blood feeding arthropods and not else.
In ants or they didn't find'it yet.
Or they have a immunity.
Thank you
  • asked a question related to Mosquitoes
Question
5 answers
I'm working with a mechanistic model to predict mosquito abundance with temperature input. I also have a raster with land-use classification for the same area and I now want to know how land-use dictates mosquito abundance (as land-use influences micro-climate). I think I should correct for spatial autocorrelation, but am struggling with how to do that best. One of my ideas is to find out up to what lag distance autocorrelation is present/significant and then do a ANOVA+post-hoc only on cells sampled x lag distance apart.
However, I'm confused about the different approaches amongst different functions/packages in R. On the one hand I tried lm.morantest, which computes Moran's I over the residuals of a linear model (in my case mosquito vs land-use). On the other hand I came across the raster package function , which calculates Moran's I just over the variable values (so either autocorrelation within the mosquito raster or in the land-use raster). Also sp.correlogram from the spdep package takes a variable vector as input instead of a linear model. The latter two functions give almost double the amount of autocorrelation compared to the first method.
So, I think I understand why you would check for autocorrelation in the residuals, but why in the variable input? Part of the correlation you're finding with the latter method might already be explained by your other variables as you're doing for example in linear regression? Are there options for doing something like a correlogram but with model residuals instead of variable input?
Relevant answer
Answer
for checking for spatial autocorrelation between two variables...lookup bivariate Moran's I..in Geoda..its very easy to do. You can check our publication which uses it. http://mires-and-peat.net/pages/volumes/map26/map2604.php
  • asked a question related to Mosquitoes
Question
27 answers
During summer days mosquitoes are hugely generated to become more active in attacking people. When we do hiking a lot of mosquitoes are there. We put anti-mosquitoes spray, but it does not work well. We are wondering how much it is possible the mosquitoes have ability to transfer coronavirse among people.
Relevant answer
Answer
  • asked a question related to Mosquitoes
Question
17 answers
We are researching the recepter of Bt toxin in Aedes aegypti, and I would like to know if anyone can provide the cell line of Aedes aegypti (eg. Aeg 2), or tell me where and how can I get it.
Relevant answer
Answer
Hi Songqing,
I was wondering if you managed to get Aag-2 cell lines.
I am also in the need of Aag-2 cell lines. I would appreciate if you can share that with me or tell me the source.
Thanks,
  • asked a question related to Mosquitoes
Question
11 answers
Does the mosquito larvae hibernate?
I am surprised that one week ago, I dried a pool full of larvae and sprayed some 95% ethanol. They have been dried and stayed immobile since then. But today, I poured some water, to my surprise, they are alive again! stretching legs and bending over!
Relevant answer
Answer
I feel that you are using the term "Hibernation" in a wrong context. Hibernation mean an organism slows down its metabolic processes to tide off the adverse climatic condition. And for sure in temperate regions (having extreme cold conditions) many species of mosquitoes hibernate in larval stage for a long time (months together) and with the onset of favourable climatic conditions they come out of hibernation and resume the normal life cycle. In your case, perhaps what has happened is that even after draining the water of the pool some moisture might have been left out which some how or the other helped larvae to survive and with the availability of water they revived themselves.
  • asked a question related to Mosquitoes
Question
3 answers
I have a botanical extract which was prepared through maceration method. The powdered plant material has been soaked in pet-ether solution. The final product after filtration and drying is a very thick paste. I want to test this product against mosquito larvae. I hope someone who have experience working with pet-ether could share your knowledge. Thanks...
Relevant answer
Answer
Dear Manorenjitha M.S. if you add little quantity of an organic solvent to your solution that it will affect the tests
  • asked a question related to Mosquitoes
Question
18 answers
I need a proper protocol for RNA isolation from mosquito samples i.e. either larval, pupal or adult stage.
Relevant answer
I will do some tests using Trizol + RNeasy mini kit to extract RNA from single mosquitoes. I will homogeneize the mosquitoes using the tissuelyser, it's great.
Most papers I read use pools of tissues or pools of whole mosquitoes ranging from 5 to 20+ speciemes/pools. Sierra Schluep , any updates on your results? Thanks.
  • asked a question related to Mosquitoes
Question
6 answers
I have collected some mosquito larvae from different breeding sites in Egypt. Most of them are native to Egypt. I found in on place, larvae look like Culex torrentium according to the classification key of Harbach (1985) (saddle seta 1-x and seta 1 on abdominal segments 3-5). However, we know that this species is not present in Egypt. So, I appreciate if a mosquito taxonomist can look at the attached captures to tell me the right classification of this larva and whether it really belongs to Cx. torrentium or to Culex pipiens.
with regards,
Wesam
Relevant answer
Answer
Dear Wesam Salaheldien Meshrif
You can get help to identify the mosquitoes by the key " The fauna of British India including Ceylon and Burma'' by Christopher (1933). Please see attached file. thanks
  • asked a question related to Mosquitoes
Question
5 answers
i want to know how experimental hult trials works
Relevant answer
Answer
Check the attached file for all details of mosquitoes collection in experimental huts, Martin Chiumia @ Martin Chiumia
  • asked a question related to Mosquitoes
Question
10 answers
If it spread by rat or mosquito then special precautions and control measures urgently needed. In a news, another virus i.e. "Hantavirus" is spread through the rat.
Relevant answer
Answer
Still there is no report regarding this.
  • asked a question related to Mosquitoes
Question
8 answers
I will be rearing a colony of wild-caught Aedes albopictus at a remote field site where access to, and storage of fresh blood for artificial feeders will be inconvenient. It has been suggested to me that I should live feed the colony using domestic chicks (which we could safely/humanely house at the field sites), however I've failed to find a single protocol that describes this method. Has anyone used this method before, or know of a published protocol? 
Relevant answer
Answer
Dear Priscilla,
Of course - I will send that to you now. It is worth noting, however, that this protocol was developed for use in remote field sites, so you may find laboratory protocols (e.g. from Benedict, M. Q. (2007). Methods in Anopheles research. Malaria Research and Reference Reagent Resource Center (MR4)) more suited to your work.
All the best,
Nichar
  • asked a question related to Mosquitoes
Question
1 answer
I want to deliver dsRNA into adult mosquitoes thorax using a WPI microinjection system. There were a lot of pre-pulled needles are available with varient inner and outer diameter. Can anyone suggest me, what will be the best needle size for the purpose of injecting adult mosquitoes?
Relevant answer
Answer
Hello,Banerjee
Drummond Scientific 3-000-203-G/X Replacement Glass Capillaries for Nanoject II Auto-Nanoliter Injector, 3.5" Length work very well for mosquito pupa injection
  • asked a question related to Mosquitoes
Question
34 answers
Is it possible that Mosquito start acting as VECTOR for all or some strains of Covid-19,if not,why? any scientific/Biological reasoning in favour or against this thought.
Relevant answer
Answer
Not all, but it can often be
  • asked a question related to Mosquitoes
Question
1 answer
The research idea is to study the fecundity and sporogonic development of wild mosquitoes. Apart from the low fecundity as a result of parasitaemia in the vector, could there be another association to look out for?
Thank you
Relevant answer
Answer
The environment can have a role in that
  • asked a question related to Mosquitoes
Question
5 answers
I am working on mosquito classification and identification and to do this I use many identification keys, I need to improve my skills and accelrate my work so Ican finish my work quickly and accurately, to do that I need to reduce the altering between the Keys.
Relevant answer
Answer
Just to make sure you found this updated key for Anopheles:
  • asked a question related to Mosquitoes
Question
5 answers
A few months ago there was a chikungunya outbreak in some parts of Ethiopia. Chikungunya is one of the mosquito transmissible viral infections.
Relevant answer
Answer
Diagnostics: PCR, serology
Treatment: Paracetamol, symptomatic, no ASS
  • asked a question related to Mosquitoes
Question
5 answers
I want to use imageJ to count the number of eggs in Gypsy Moth egg masses, but I am not sure what camera and camera lens I would need in order to do this. I have seen a couple of mosquito papers that do this using a DSLR with a macro lens, but they do not provide any other specifics. Any thoughts or suggestions would be much appreciated. Gypsy moth eggs are ~1mm in diameter.
Relevant answer
Well it's depends about your budget. An inverted microscope with 600X is around of US$ 3000-4000 (medium gamme), I use a Optika XD-3 and take good photos, other option are the Dinolite digital microscopes around of US$ 2500 (puted in Ecuador) this last is so easy to take photos and to take measures. And the mercedes benz are the Nikon inverted microscopes but are expenses more than US$30.000
  • asked a question related to Mosquitoes
Question
3 answers
I am using Universal primer set (LCO1490 and HCO2198) to identify Culex mosquito species (tritaeniorhynchus and quinquefasciatus) by using COX gene. the expected product size was 710 bp but after gradient PCR. when i ran samples on gel i got two bands for each sample. i.e at around 300 bp and the other one was at around 500 bp. GC content for both primers is < 35%.
Relevant answer
Answer
There was probably a problem with the thermocycler programme.
  • asked a question related to Mosquitoes
Question
6 answers
Good evening my name is Marta Albani, I am working at the Parasitology Laboratory of Sapienza University of Rome; I'm just working on insects increase number for a future male release after sterilization; I need methodological information about the blood feeding for captive females.
Regards
Dr. Marta Albani
Relevant answer
Hello Marta,
I suggest the literature below, which uses a simple system to obtain. Good luck.
Rutledge LC, Ward RA, Gould DJ. Studies on the feeding response of mosquitoes to nutritive solutions in a new membrane feeder. Mosq News. 1964;24:407-19.
  • asked a question related to Mosquitoes
Question
2 answers
I'm trying to figure out a way to ship mercurochrome-stained mosquito midguts for Plasmodium oocyst isolation. I need the samples not frozen. Does anybody have shipped them before? How did/would you do it?
The shipment will be overnight. We have tried with RPMI but the midguts were all mushy and unworkable at the arrival. We also tried with PBS and they looked a little better. But any better solution you can suggest is more than welcome!
Relevant answer
Answer
Thank you for your answer, Himmat!
What kind of isotonic medium?
  • asked a question related to Mosquitoes
Question
2 answers
Hello,
We recently obtained some insect samples (Asphondylia) collected a few months ago by collaborators. The individuals were freeze dried, placed into pure ethanol, and shipped to us on dry ice.
I am having trouble getting a good DNA concentration from them (~50ul, 0.5ng/ul). I have tried the Qiagen DNeasy blood and tissue kit, phenol chloroform, and the Lucigen MasterPure Complete DNA & RNA Purification kit. I have tried varying incubation times (up to 17 hours) without any increase in DNA yield. I've been using the Quiagen Tissuelyser with tungsten beads to ensure thorough homogenization.
Does anybody have any protocols or suggestions on how we can get a higher yield? They are quite small insects (like mosquitoes), but I need a minimum concentration of about 5ng/ul in 150ul!
Thanks!
Relevant answer
Answer
Hi, I'll try to rehydrate my insects and add some distilled water (1/1 volume) in my tubes before the extraction to dilute ethanol. Then discard supernatant, and add water again and again.
Good luck :)
  • asked a question related to Mosquitoes
Question
5 answers
I am doing mosquito larvicidal activity against different concentrations of temephos to determine the resistance status in the field population. During the larval bioassay, I have been noticed that few mosquito larvae were formed to pupae and those pupae were live even at higher concentrations of temephos.
Please any one share is temephos has pupicidal activity or not. If not, why ?
Relevant answer
Answer
The activity of enzymes potentially involved in insecticide detoxification (glutathione-S-transferases, GST; esterases, EST; and mixed function oxidases, MFO) is different at the various stages of development.
  • asked a question related to Mosquitoes
Question
6 answers
methods of collection and preservation
tools needed
Relevant answer
Answer
BG-GAT traps
  • asked a question related to Mosquitoes
Question
3 answers
Does anyone know what the contaminant in this nanodrop image could be? Its from a phenol/chloroform/isoamyl extraction of a single mosquito larva. I washed twice with an excess of chloroform. The ethanol precipitation was carried out using 100 ul of aqueous layer in 0.3 M sodium acetate and 1 ul polyacryl carrier in over 1 ml of 100% ethanol. The pellet was washed once in 70% ethanol and was a light grey color. I resuspended in 20 ul of water. Thanks.
Relevant answer
Answer
I'll wait with you answer . ..
  • asked a question related to Mosquitoes
Question
12 answers
  1. Rearing the target mosquito species for a susceptibility test is tedious task and expensive compared to intermittently catching the target species.
Relevant answer
Answer
for resistance tests it is better to make them with mosquitoes captured in the field, and if it is possible to carry out the resistance tests in situ, either with impregnated wall and cones or with impregnated paper sheets, laboratory mosquitoes if they are of reference strains The knocked out is very high compared to the field, the pressure field mosquitoes have generated resistance to insecticides through traces or metabolites, so I recommend field insects@
  • asked a question related to Mosquitoes
Question
18 answers
Some papers (eg Saudi Epidemiology Bulletin 1995; WHO report 1995) report the presence of Aedes albopictus in Saudi Arabia, but there could be a mixing up with Aedes unilineatus. Can anybody confirm the presence of Aedes albopictus in Saudi Arabia and in Yemen? Alternatively, can anybody send some specimens that look like Aedes albopictus for morphological or molecular identification?
Relevant answer
Answer
Dear Dr. Francis,
Sometimes ago I wanted to answer this ques., but I thought it was out of date to answer and no longer needed. Now I noticed some colleagues are still commenting, so I must give my opinion. Regarding the existance of Ae. albopictus in Saudi Arabia, I strongly beleive that it is not prevalent in this country. I worked there for more than 20 years (1999 - 2019) surveying and identifying mosquito species in various parts of the country. I have never ever come across a single specimen of this species. During this time, I identified more than 50 000 mosquito specimens (adults & larvae) as Ae. aegypti beside other species searching for albopictus without success. Those, who claim that they found specimens of albopictus, need to confirm their finding and further surveys need to be conducted to prove their presence.
Thank you....
  • asked a question related to Mosquitoes
Question
6 answers
Hi,
I want to preserve my field caught dengue mosquitoes in RNAlater solution until RNA extraction later. Before that, I want to identify and sex them. Since identification of live mosquitoes would damage their body parts, i am going to cold anesthetize them at 4 celcius for about 10 minutes and do the sorting. Once it is completed I am going to preserve them in RNAlater and put them in the freezer. I want to know if it is ok to add the cold mosquito to RNAlater. Would it do any degradation to the RNA of the mosquito?
if you have any better option, please suggest.
Thank you
Relevant answer
Answer
Dear Dhanusha
Do you use home made RNA later or from the company (?)
I want to keep my insect samples in RNA later for next generation studies (NGS), but not sure which company has suitable RNA later (off course not a expensive one) for this purpose
thanks in advance
  • asked a question related to Mosquitoes
Question
1 answer
Hello,
We use Qiagen's QIAcube HT for RNA extraction from mosquitoes for our state surveillance of West Nile virus. We have spoken with other labs that do this, but they place their mosquitoes in microcentrifuge tubes and pipette off supernatant from those tubes. We, however, were convinced to use Qiagen's CMT racks (collection microtubes-racked) in place of microcentrifuge tubes as they can streamline the procedure by allowing the use of multi-channel pipettes and the ability to bead beat a full 96 samples at one time (the QIAcube HT can also pipette the supernatant directly from CMT rack tubes but that would not work for us for various reasons). The CMT rack tubes are much narrower than typical microcentrifuge tubes and we have to centrifuge the entire CMT rack (rather than individual microcentrifuge tubes) which makes it harder to produce high g-forces. These factors result in a supernatant that is not as clean as we would like. When those not so clean supernatants are ran through the QIAcube HT filters (we specifically use the RNeasy kit), some of those filters inevitably clog. We asked Qiagen if any other labs use the QIAcube HT plus CMT racks for mosquitoes but we typically received ambiguous answers or were connected to a lab that uses the QIAcube HT for mosquitoes but not CMT racks. Basically, I think we have realized that we are the guinea pigs for this particular combination. So I was curious if anyone else was having clogging issues with the QIAcube HT regardless of their starting specimen or protocol, and what they did about it? I will say about halfway through the WNV season I came up with a method that quickly gets rid of the clogs once they appear but I would rather they not appear in the first place. I found it was possible to wash the top of the clogged filter with the standing liquid atop the filter by carefully pipetting up and down with a 1000uL pipette (smaller tips are too pointy and could damage the filter). The particles causing the clog are not large enough to see with the naked eye but this process definitely redistributes them as the standing liquid almost immediately goes through the filter (the vacuum is running during this process). Thank you for reading.
Relevant answer
Answer
Hi.
I met this problem as well.
After consulting with the engineer from QIAGEN, the problem is not perfectly solved but the possible reason could be the viscosity of the sample. Dilution with PBS or decrease the amount of sample adding could be a way out.
  • asked a question related to Mosquitoes
Question
12 answers
I have conflicting information about the methods used to raise Anopheles funestus mosquitoes in the lab. Has anyone successfully managed this? Would you please share your experiences and/or protocols?
Relevant answer
Answer
iH
  • asked a question related to Mosquitoes
Question
7 answers
We have infected mosquitoes silenced for a specific protein with Zika virus . After a number of days we dissect the midgut, make a homogenate and try to check for virus replication by plaque assay.
Our plaque assay in the first dilution (1/10) shows complete lysis of the cells (and it doesn't look like plaques) in the second dilution (1/100) there is nothing. We have used just the midgut of non-infected mosquitoes to check if there was something in the midgut that would just kill the cells and nothing happened. We still have to make twofold dilutions... Thanks for thoughts on that!
Relevant answer
Answer
Carla,
I guess you prepared the silenced and non-silenced mosquito the same way so it's strange that the control and experimental group did not have the same problem. I agree with Barry that the problem might come from contamination. Did you observed turbid overlay medium after incubation?
Also What is the infection titers in the control group and how many mosquito samples have you done with the plaque assay?
  • asked a question related to Mosquitoes
Question
4 answers
A 1969 paper reports prevalence of Zika virus in Malaysia (Marchette NJ, Garcia R, Rudnick A (1969) Isolation of Zika virus from Aedes aegypti mosquitoes in Malaysia. Am J Trop Med Hyg 18: 411–415).
Relevant answer
Answer
Please see the following RG links and PDF attachments.
  • asked a question related to Mosquitoes
Question
2 answers
I tried to optimize conditions but havnt find any way. When i use mosquito cell line C6/36, target protein band comes at 50. But with live mosquito never got this. I attached membrane picture also please guide me how i can solve this issue Thanks.
Relevant answer
Answer
As Mahesh already suggested, loading a control protein is always a good idea. You would easily see if your PA gels are running differently for any reason or if it has to do with the protein itself.
The different protein sizes could arise from your sample preparation. Maybe there is proteolytic cleavage in the mosquito preparation.
As you did not describe the sample preparation, I assume the protocols for cells and live mosquitos differ.
Have a look at your lysis protocols for the cells and the whole organism and compare the procedures. Make sure to include precautions against degradation in the live mosquito protocol. In general, always precool your solutions to 4°C, work on ice and add protease inhibitors or a inhibitor cocktail to all your buffers once the cells are disrupted.
Some chemicals like imidazole also show proteolytic activity, so check your buffer compositions for potential candidates.
For example, if your protein is tagged and the protocol includes an immobilized metal affinity chromatography as purification step (IMAC, usually Ni2+ or Co2+) with elution by imidazole-containing buffer, do not store your samples in the elution buffer. Remove imidazole by dialysis or ultrafiltration. From my experience, this is a crucial step and I would not store a protein in imidazole even if the samples are frozen at -80°C.
Finally, check if the samples used for PAGE have the same composition and are denatured in the same way. If the buffers of the cell and live mosquito preparation differ a lot, i.e. in ionic strength or maybe detergent content, this could lead to different electrophoresis patterns. I would then recommend to match the buffer composition by removing salts or detergents by dialysis or gel filtration.
I hope these suggestions give you starting points for troubleshooting!
Best, Marianne
  • asked a question related to Mosquitoes