Science topic
Morphine - Science topic
The principal alkaloid in opium and the prototype opiate analgesic and narcotic. Morphine has widespread effects in the central nervous system and on smooth muscle.
Questions related to Morphine
I want to study the effects of a pharmacological treatment (antidepressants) related to quality of life in oncologic patients. Apart from a depression diagnosis that would be a prerequisite for administering the treatment, i need another screening tool that could confirm the patient's ability to be functioning enough to give me true and valid answers later in the main tests. For this reason I am looking for a validated tool in clinical setting that could detect any cognitive impairment due to a psychiatric condition or substance induced (es. high doses of morphine).
I want to design an aptamer for morphine by in sillico method and i need some basic sequence but i can not find them. if you have a library or know some article that can help me , please introduce me. thanks
Hello All,
First I want to start this discussion by thanking you for helping me with this as it is something I have been struggling to figure out.
I picked up this study from a graduate student before and now I am stumped by how to run the data using either SPSS or R (preferably R) or another recommendation. I have attached the data I am working with and as you can see it is divided into three treatment groups: Saline, 10mg/kg, 20mg/kg. By Sex: Male & Female and across three time periods: 12HR, 24HR, 36HR. I was looking at withdrawal scores and in particular certain attributes associated with withdrawal in the rat which is 11 items. What would be the best method to run this data if I want to look for effect of treatment and sex differences on the withdrawal behaviors?
Thank you again.
Currently, I’m studying the cellular Ca2+ responses, induced by morphine or fentanyl, of HEK293T expressing μ -receptor, in which 4μM Rhod-2-AM is loaded as a fluorescent Ca2+indicator. The Ca2+ signaling is investigated by the FLIPER PENTA (Exc. Wlength: 510-545 nm; Em Wlength: 565-625nm; Gain: 100; Exp. Time: 0.2s). As reported in many pieces of literature, the intracellular Ca2+ decreased obviously after adding cera tain concentration of morphine or fentanyl, indicating an inhibiting effect on calcium influx (A-B). However, a high and sharp peak appeared in the next few minutes, and I could not correctly interpret its biological significance by consulting the existing literature. The same phenomenon exists in all other wells (C-D). Have you ever found a similar phenomenon in your experiment? I would appreciate it if you could give me some directions.
Recently several babies exposed to methadone and benzodiazapines have been overly sleepy with minimal interest in feeding, extremely gassy and are over a month of age and already off morphine and clonidine treatment for NAS.
Repetitive opiate drug usage results in physical dependence which is approved by the appearance of withdrawal symptoms. withdrawal symptoms are dividing into two groups; Checked and Graded signs.
I would really appreciate it if I get guidance related to this issue.
Another issue is what are the pros and cons of using this kind of assessment?
I want to purchase some Morphine Standard for HPLC to detect Morphine rate in Papaver somniferum L. how much is need in every stage or phase?
I am currently working within ambient ionisation mass spectrometry, specifically thermal desorption APCI and looking at the linear range of my analytes using the calibration range 10-50 ppm with increments of 10 ppm. I am working in positive mode using selected ion monitoring for each analyte, as it's a single quadrupole mass spectrometer.
I have experienced a significant reduction in analyte signal for amphetamine and morphine from 10 ppm to 20 ppm. The response at 10 ppm was approximately 3.00e7; however, when I test 20 ppm the signal is circa 2.00e4 and remains as so for 30-50ppm. I am using aluminium foil as my sample swab material and when the sample is pipetted onto the surface, it disperses but the covered surface goes into the thermal desorber oven so I would expect full desorption of the analyte.
I'm stumped at this point and would appreciate any help.
Thanks for reviewing the animal studies of the 1970's and especially for describing the results of our early study that did not find that enriched rearing led adult rats to avoid self-selection of morphine and cocaine. Your review shows that the effects of early environment are complicated and also demonstrates the importance of caution in interpreting such studies. An alternative interpretation to that of Alexander, based on our results might suggest that too much stimulation in the early environment may lead adult animals (rats and humans) to continually expect and seek such stimulation by changing state through use of drugs. Could it be the case that children in today's world with an enormous amount of screen time (cell phones, internet, TV) may be at a disadvantage from the standpoint of susceptibility to drug use later in life?
Beside color marker injection, is there any pharmacological agent (beside morphine) that induce a particular phenotype (morphine Straub tail) in mice? thanx in advance Roland
I'm currently studying perinatal opioid exposure in rats. We've been using morphine pellets, but are considering switching to osmotic minipumps. However, we want to ensure that our dosing is as clinically relevant as possible. Is there a formula or suggested conversion for human effective morphine doses to their equivalent rat dose? I've been considering both equivalent plasma levels and equianalgesic doses, but I wondered if the data already exists somewhere. Thanks!
We synthesized highly purified thalidomide by ourselves and we have treated many patients with malignancies using thalidomide and celecoxib with good efficacy. But in Shizuoka Prefecture, I was ordered to cooperate the recall of thalidomide by the President of Shizuoka Prefecture(=Shizuoka Ken) because thalidomide synthesis is against the Law.. Our works are cited on many famous medical journals and textbook overseas. Japanese Government ordered not to conduct clinical trials for advanced patients with solid tumors. Because thalidomide is the dangerous drug and difficult to keep the patient safety. Moreover, many advanced cancer patients are refused to treat more after standard chemotherapy at Shizuoka Cancer Center, even if they could be estimated to recover with thalidomide and celecoxib. Please cooperate to help Japanese advanced cancer patients save their lives from Shizuoka Concentration Cancer Center. Without your helps, they have to die with the cease of respiration after injections of overdose morphine hearing the stream sound of high volume oxygen.
The present President of Shizuoka Prefecture is Heita Kawakatsu and Shizuoka Cancer Center is Ken Yamaguchi.
Masato Hada
When digested, AI milk releases beta-casomorphin7 (BCM7), an opioid with a structure similar to that of morphine that some studies have linked with autism. Does anyone have information on the mechanism of BCM7 formation?
Hello... Anthony Foris
In vitro studies have shown that, the bioactive peptide β-casomorphin-7 (BCM‐7) is produced by the successive gastrointestinal proteolytic digestion of β-casein A1, but such cases were not seen in β-casein A2 type milk.
Kindly see these two articles:
Pictorial representation made by me is also in attachment.
Hi guys
Does any one have the full text article for " Identification of Possible Binding Sites for Morphine and Nicardipine on the Multi-drug Transporter P-Glycoprotein Using Umbrella Sampling Techniques "???
Authors:
- Nandhitha Subramanian
- Karmen Condic-Jurkic
- Alan E Mark
- Megan L. O'Mara
Thanks in advanced
Fatemeh
Considering the small size of the molecules, an association with bigger immunogenic protein is necessary (BSA, KLH, TT).
1- is a spacer necessary for a better recognition of the antigen (thinking of succinic acid)?
2- what are the best animal models to be chosen (mice, rabbits or others)?
3- After antibody purification, what is the stability of the retrieved antibodies? is it possible to use them for other detection methods (ELISA, WB etc)?
Some patients on opiate or opioid analgesics paradoxically react with hyperactivity, verbosity and insomnia. As a student (more than 50 years ago) this was demonstrated during the pharmacology lectures bij injecting a cat with morphine and show it a white mouse, whereupon the cat panicked and jumped up and down its cage. Our pharmacology professor then explained that 'a small percentage of the human population reacted like cats', and indeed, this is reported incidentally by patients.
Can anybody explain the incidence and mechanism of this paradoxical effect?
I am looking for a protein that it's conformation changes specifically by binding morphine molecule to it.
Is there such protein? or are there any software change a protein to that bind specifically to morphine?
Thank you
Eleonore Dorothea Auguste Henriette von Rettberg was the wife of Friedrich Sertürner, who isolated the first alkaloid in 1804; morphine from opium. Did Frau Sertürner die of a morphine overdose? Any leads would be most welcome.
This question is for the people practicing outside USA because in USA this number is quite high. I am doing some international work andI am just interested in OB anesthesia practise outside USA.
When you provide anesthesia for Cesarean Section what percentage of your patients receives spinal or epidural morphine?
This is questions for practitioners from outside USA, because morphine chloride is not available in USA
The WHO analgesic ladder has weak opiods like codeine and tramadol, however they are expensive and not easily available. Do you have any experience on the use of low dose morphine instead of the weak opiods
The Driving Authority in the UK (DVLA) has set a limit for morphine of 80 mcg/litre plasma concentration. Does anyone have any figures giving an idea what this equates to in dose terms for palliative patients?
Have you ever heard of Midazolam being used in conjunction with morphine for the treatment of MI chest pain, and associated synergistic benefits?
I am studying research surrounding the use of intranasal analgesia (fentanyl or diamorphione) for paediatric pain in pre-hospital settings as opposed to IV/IM morphine or other weaker analgesics. Any paediatric pain scoring models would also be of assistance, or research surrounding the above topics.
Methadone maintenance is in the patients perspective a quiet "boring" drug. We think that not only cocaine is often used in high dose maintenance, but also alcohol (carbonic acid + alcohol = "cick"). My question is:
Does anybody know wheher there are combination maintenance programs with fast acting morphine in daytime and low methadone for the night?
regards
c. jellinek
Dexmedetomidine is used as an adjuvant to intrathecal local anesthetic (Bupivacaine/ Ropivacaine ). Does it help in prolonging duration of spinal anesthesia? How would you rate it on comparison to fentanyl/ morphine?
I see many "drug babies" born to women in their late 30's. A majority of the women are using Subutex (Suboxone) or Methadone as a means to come off other drugs. Babies usually score 6-10 in the first 24 hours but can jump as high as 44 in the 24-48 hour range depending how much mom was getting. We want the babies to be on high calorie formula, but should we use mom's breast milk to dose the baby instead of the morphine?
In terms of analgesic effects.
