Science topic

Mollusca - Science topic

A phylum of the kingdom Metazoa. Mollusca have soft, unsegmented bodies with an anterior head, a dorsal visceral mass, and a ventral foot. Most are encased in a protective calcareous shell. It includes the classes GASTROPODA; BIVALVIA; CEPHALOPODA; Aplacophora; Scaphopoda; Polyplacophora; and Monoplacophora.
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The "konokono", from the local language in Mauritius and Rodrigues, is a really well known seafood delicacy (small to medium size sea mollusk with a shell). However, I really did not manage to find any scientific related information based on this local name (and we are not here speaking about a snail which lives in Africa and is called also konokono in swahili language).
I join here pictures of one same specimen, supposed to be a konokono.
I am looking for the scientific name of this mollusc species.
Any help would be really appreciated, thank you.
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Pleuroploca trapezium?
Thanks!
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Hi all,
I am looking for type material of fossil terrestrial gastropods described by August Reuss. Specifically I want to find the syntypes of Vertigo callosa Reuss, 1849 from the Early Miocene of Tuchorice (CZ). Reuss worked, amongst others, in Prague and Vienna, and parts of his collections (microfossils) were purchased in 1891 by the Natural History Museum in Vienna. However, the mollusks aren't there, and I couldn't find any information on their whereabouts, apart from a few author's statements that the collection "might be lost". It remains uncertain though if anyone actually made a serious effort to locate it.
Any hint or suggestions would be most welcome!
Best wishes,
Thomas
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Dear Marc,
Thanks for the tip! I contacted them, hopefully something will turn up.
Best wishes,
Thomas
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Dear fellows, the GenBank lists only 3 vouchers for Theodoxus fluviatilis in Crete, from Stylos springs SE of Chania, and from the outflow of Kournas lake, SE. of Georgioupoli.
But the Theos from the "Almyros" karst springs of Georgioupoli and Gazi are not documented. It is said here and there, but without evidences, that they belong to fluviatilis; but their opercula show features that are, IMHO, somewhat different from those of average fluviatilis.
Any infos about these snails, somewhere?
Attached: three images of the Theos from Gazi and Georgioupoli, collected in the springs and in a river in the vicinity.
Thanks for any help!
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Abhijit Mitra Thank you! I have now to find the paper given by Szerkowski in 1998 about the residual populations in Crete.
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1: foot. 2: anterior cephalic lobe. 3:posterior cephalic lobes held erect. 4: pallial exhalant siphon; taper fits adapical angle of aperture. 5: glans of penis? 6: rectum and anus OR female opening/vagina?
I have used Fretter and Graham as a guide but features on a live specimen differ from those on their dissected dead specimens. [ F & G, 1954. Observations on the opisthobranch mollusc Acteon tornatilis (L.) J. Mar. Biol. Ass. 33(3)].
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Nathalie I think you may have missed that my previous response was in two messages as the first answered your location query.
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Dear Colleagues:
These papers are very old. If you have a copy, please share with me.
Kind regards.
Subhronil
List:
=======
Kapitza, A. A. New species of lower Cretaceous inoceramid from lower Priamur. 65-77. In: Poyarkova, Z.N. (ed.). Biostratigraphy of the south of the Far East (Phanerozoic). DVNTS AN SSSR, Vladivostok. 139 pp.
M. M. Astafieva. 1989. On the representatives of the genus Maitaia (Bivalvia). Paleontological Journal 23(3):11-19
Keller, S. (1982). Die Oberkreide der Sack-Mulde bei Alfeld (Cenoman-Unter-Coniac). Lithologic, Biostratigraphie und Inceramen. Geol. Jahrb., 64, 3-171.
Heinz, R. (1932). Aus der neue Systematik der Inoceramen. Mitteilungen aus dem Mineralogisch-Geologischen Staatsinstituts in Hamburg, 13, 1-26.
Marwick, J. (1953). Divisions and faunas of the Hokonui System (Triassic and Jurassic). Geological Survey of New Zealand, Palaeontological Bulletin, 21, 1-141.
Chen, J. (1987). Early Jurassic marine bivalves from Guangdong-Nanling district, southern China. Bulletin of Nanjing Institute of Geology and Palaeontology, Academia Sinica, 12, 23-94.
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Please can you name the red marks labelled 1 and tell me their function?
The specimen has the roof of the nuchal cavity removed. The visceral hump still has the mantle but some viscera have burst out. Am I correct in thinking that the spheres labelled 2 are balls of food in the stomach before being compressed in the intestine into faecal rods?
From Mersey Estuary, England. Shell length 41.6 mm.
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Thank you Fareeda Tahir for your response. I am sure that the red items 1 are not the gills or ctenidia. Patella species have no ctenidia. Instead they have pallial gills around the perimeter of the foot. They are labelled 4 in this image. The white ‘v’s show the route of colourless haemolymph depleted of oxygen passing from the body into the pallial gills. The blue ‘V’s show the route of oxygenated bluish haemolymph from the gills, through the efferent branchial vessel (5) to the nuchal cavity (7) where the heart pumps it to the organs in the body and head.
My question was whether the spheres are food balls. The digestive gland is very large in patella and is not the spheres. It is labelled 2 in the image at https://flic.kr/p/Bex7R6
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Could anyone help me in identification of these species growing in China's coastal wetlands? I attach some photos. Thank you.
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  • They appear to be specimens of Assimineidae. I am unable to identify the species.
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Is their specific threshold for discriminating species similarity based on Mitochondrial DNA Percent similarity Identification particularly for Mollusk? What is specific DNA barcode (BLAST) Threshold or standard similarity to ascertain if the two species belongs to the same genus or if they belong to similar or different species?
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Hebert et al. (2003) established a 2% threshold for intraspecific variations. However, they used many different taxa in their study. Based on my opinion, the threshold should be group-specific since 2% does not work in other taxa.
Similarly, do not just rely on molecular sequences for species delimitation and better to integrate it with morphological and ecological data.
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I regularly have low DNA concentrations on the vetigastropod tissues I extract. I have tried fresh tissue and older/museum tissues. We use the Thermo Scientific GeneJET Genomic DNA Purification Kit.
Heating the elution buffer and using less buffer to elute does help, but concentrations are still quite low. Downstream applications are PCR and sequencing.
Super desperate and would appreciate any suggestions on how to increase the yield from extractions!!
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Thank you!!
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Hi All,
I am trying to amplify mitochondrial 16S gene for marine snails (Calliostomatidae) and other vetigastropods, but I only get primer dimers or nothing on the gel. These primers have worked previously in my lab and in numerous other publications. The DNA concentrations are low, but they have amplified for COX1 using the Folmer universal primers.
I am using the Palumbi 16S universal primers (Forward: CGCCTGTTTATCAAAAACAT and Reverse: CCGGTCTGAACTCAGATCACGT). We bought new primers in December 2023. I resuspended them and have tried multiple aliquots. I've tried gradients 45-55 and 55-65 and a touchdown PCR starting at 59 (-1 C/cycle for 10 cycles) and final annealing at 48 for 20 cycles. I've tried the standard, ammonium, and combination 10x buffers. All reagents are from Apex (not a hot start taq), except for the dNTPs.
Our usual protocol is: 2.5 uL of 10x standard buffer, 1.25 uL of MgCl2 (50mM), 0.5 uL of dNTPs (10mM), 1 uL of both primers (10uM), 0.2 uL of taq (5 units), and 2 uL of DNA. This does work for Folmer. Denaturation at 95 C for 4 min, 35 cycles of 95C for 30 seconds, 50C for 30 seconds, and 72C for 30 seconds, and final extension at 72 for 10 min.
I'm desperate and would love to hear any suggestions/tips on how to fix this!! I also unsuccessfully tried ethanol precipitation to increase the DNA concentrations, so tips on that would be appreciated too. Thank you
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Dear Tiffany
I checked in Primer BLAST. Primers can indeed amplify the snails. See first two results:
Products on target templates
>MF979281.1 Calliostoma unicum isolate DYLKL2 16S ribosomal RNA gene, partial sequence; mitochondrial
product length = 586 Forward primer 1 CGCCTGTTTATCAAAAACAT 20 Template 1 .................... 20 Reverse primer 1 CCGGTCTGAACTCAGATCACGT 22 Template 586 ...................... 565
>NC_068865.1 Tristichotrochus unicus mitochondrion, complete genome
product length = 585 Forward primer 1 CGCCTGTTTATCAAAAACAT 20 Template 10736 .................... 10717 Reverse primer 1 CCGGTCTGAACTCAGATCACGT 22 Template 10152 ...................T.. 10173
But Tm is 52 and 62 for fp and rp by primer blast and 59 and 68 by Thermofisher's online multiple primer analyzer.
Additionally, not a cross dimer but the forward primer forms dimer very easily (Thermofishers online multiple primer analyzer).
See the results:
1 dimer for: f
5-cgcctgtttatcaaaaacat->
||||| | | |||||
<-tacaaaaactatttgtccgc-5
You said you used 50C as Ta. I have not done pcr for the snails ever, but I may say what I may have tried:
1. Increase the Ta, as increased Ta will decrease the chance of self dimer of forward primer (will be become unstable). May be, use 55-60C.
2. You use 1.25 mcl of 50mM MgCl2 in 25 mcl reaction, this leads to final 2.5 mM. Usually the PCR buffer has MgCl2 to make final 1.5 mM. Thus the sum if 4 mM. Increased MgCL2 increased the stability of duplexes, eg primer dimer. So either dont add the MgCl2 (if the PCR buffer is known to have it). Or if PCR buffer doesnt have it, add only to make final 1.5mL, eg. add 0.75 mcl per 25 mcl reaction volume.
3. If primer dimer persist, you may want to decrease the concentration of primers to 0.5 mcl or 0.25 mcl per reaction.
4. All these methods (1-3) decrease chance of primerdimer but also decrease chances of amplification (though slightly lessly), so increase the cycle number, if you do 1 or more of these changes.
Hope your experiments go well and you may get better answer. Paul Rutland is a retired Oxford? lab tech and he loves to answer such question in Researchgate. He may give you answers, much accurate.
Paul Rutland (researchgate.net)
Divya
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while attending class my professor asked us to count benthic species,and in my counting i mentioned sea snails,certain i have read multiple times that their classification is "univalvia" since their shell is made from a single piece,unlike the two shells of BIvalvia. Now howerver,i see the term is used as arhaic?
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Those mollusks that have shells, have only a single shell. Bivalves do not have "two shells", they have two valves or...BIvalvia. Thus gastropods were considered UNIvalvia (univalves). The clams, mussels, scallops are all bivalves or Bivalvia. The shelled shails are univalves but you are correct that snails are not taxonomically "univalvia" but are Gastropoda.
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Last weekend I took a photo of this cave-dwelling snail in a northern peruvian cave (1200 metres above sea level). Could you please help me to identify the Genus and Family? Thank you very much.
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The only species of terrestrial snails that resemble your species belong to the Family Polygyridae Pilsbry, 1895, which became a Subfamily by (Bouchet & Rocroi, 2005), also resembles a species of Planorbidae but these are Freshwater snails. The Polygyridae family does not appear to be distributed in Peru, it is certainly a new species belonging to a new genus. The ecosystem from which it comes (in a cave at 1200 m) is undoubtedly interesting, the specimen is albino. I advise you to go and get other specimens and keep me in mind if you want to describe the species. A malacologist, Roberto Ardovini.
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I have a E.Z.N.A.® Mollusc DNA Extraction Kit that has never been opened, but was bought in November 2018 and stored at room temperature the whole time. Is there any hope that the RNase A solution included in this kit is not completely degraded and can still be used ?
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Did it work? Unfortunately, I am facing the same issue.
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This mollusk is similar to the species Corbicula tibetensis. Or Corbiculina ferghanensis may also be. What do you think?
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I believe its a Corbicula fluminea
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  • I have come across these species so often recently that I have looked up so many literature that I have not been able to identify them as species other than the genus Creseis. Previously, I identified them as Creseis clava, but I consulted some of the original literature, which negated my decision.And their body length are relatively short, so I wonder if they are some kinds of juveniles.Could anyone answer my doubts? Heartfelt thanks!
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PTEROPODA?
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I'm having trouble obtaining a well-defined band of the COI gene in mollusk (Aulacomya atra) adductor muscle samples, despite using 35 cycles and TBT-par. Other mitochondrial and nuclear genes amplify smoothly using the same samples, such as the mitochondrial 16S gene and the nuclear 18S and 28S genes. What recommendations would you provide to optimize the PCR and achieve a clearer band?
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You can use another type of DNA polymerase for example Phusion polymerase which is highly specific and has a low elongation time with the presence of ssO7D, or even KOD polymerase which has proofreading ability, that will decrease the error rate that you can face
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Hi
Is this gelatinous mass familiar to anyone? Which genus/ species does it belong to? It was found in Norway in shallow waters close to shore, and has the consistency of nemerteans/ molluscs. It is about 2 cm in diameter. When cut in half, most of it is just gelatinous mass. However, roughly in the centre is 20-30 tiny eggs. Could it be the egg mass of e.g. nemerteans/ nematodes or molluscs?
Looking forward to your response.
Kind regards, Halldis R.
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Thank you for your reply. The "egg mass" was preserved shortly after it was found (so no hatching of the eggs).
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Dear colleagues,
On an expedition in the southern Sargasso Sea, subtropical Northwest Atlantic, I encountered repeatedly specimens of the photographed heteropod, never (so far) being bigger than 65 mm. In all cases, it has been the same species/morphotype. I greatly appreciate your expertise in identifying this species.
Plankton samples were collected in the top 300 m of the water column with a 500 um mesh IKMT.
Your input is much appreciated!
Cheers - Florian
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Thanks Matthew, I have talked with Amy Maas in the meantime about it. Cheers
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We want to adjust minimum collection sizes of bivalves for pedestrian collection in mangroves
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Hi Eduardo and Tim, I think this paper could answer your question: "Individual monitoring of gonad development in the European flat oyster Ostrea edulis by in vivo magnetic resonance imaging" (https://hal.science/hal-00572529/document).
Cheers
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they are from a mangrove ecosystem in the Gulf of Oman.
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You are welcome
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Dear colleagues,
We are currently looking for the type material of Paul Oppenheim's 1919-work "Das Neogen in Kleinasien" (Zeitschrift der Deutschen Geologischen Gesellschaft 70), specifically of Neogene non-marine Mollusca from the Denizli Basin. Oppenheim himself wrote in the work that the material is deposited in the Museum für Naturkunde Berlin, but it can't be found there. It couldn't be located in the Hebrew University of Jerusalem, storing large parts of Oppenheim's collection, either.
Does anyone know of other places that stores parts of Oppenheim's types?
Kind regards,
Thomas
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Dear Thomas, you may also check the collection of MNHN in Paris and NHM in Vien.
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These snails came with lavender (Lavandula sp.) imported from Italy to Norway. The snails are certainly not a species native to Norway. Due to the size and my initial impression, my first thought was Cornu aspersum, which is recently introduced here, but these have different patterns than the ones we typically see. I'm aware that most snails vary considerably in colour and patterns. The neat Cepaea-like lines on the underside of one threw me off particularly. The largest one is approximately 26 mm wide and 18 mm tall. The smallest is around 16 mm wide and 11 mm tall. My guess is that the smaller one is a juvenile specimen. The smaller one is also slightly damaged, which I believe is why it seems to have an open umbilicus.
Edit: They might be Eobania vermiculata. Can anyone confirm?
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This species is not (yet) established in Norway. It might be too cold for them here, but there are some warmer locations in the south where they could possibly survive. I think I will dissect out the love darts /gypsobelum and keep them with the shells for future reference.
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Dear environmentalist in Bangladesh,
I would be happy to know where I can get the FTiR microscopy facility and the already developed protocol for micro-plastics characterisation in biological samples in Bangladesh?
Also suggest any transcriptomics marker to analyse in fish and molluscs.
Thanks in advance.
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This article might help you to MP analysis. You can get a detailed idea from the corresponding author of this paper.
Jabed Hasan, can you please help him to characterize the MP.
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Hello Everyone,
I'm looking for good references that established relations between arctic zooplankton bugs' biovolume and equivalent carbon mass. Specifically for jelly organisms (Hydrozoa, Ctenophora...).
Basically, DM (dry mass) conversion to CM (carbon mass) was averaged for each group such as gelatinous organisms 15% of C, molluscs 35% of C and crustaceans 40% of C (Cotté et al., 2022), if we consider water volume for each group (considerably higher for jellyfishes).
I have only biovolume in my case (µm3 to mm3).
If anyone has good literature for common arctic zooplankton species, I would be gratefull.
Thanks in advance
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I am trying to identify the snail of the attached image. It was photographed in Cuatro Cienegas, Coahuila, Mexico by I. Domínguez.
Based on the shell sculpture, this snail resembles to the banded snail Mexithauma quadripaludium, but I have not found any information about if there is a white morphotype of this species, like the case of another endemic snail Mexipyrgus churinceanus.
I would like to identify this specimen, any help with this is welcome. Thanks for your help and comments.
A description of Mexithauma quadripaludium can be consulted on page 72 from the following document:
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Hi Eréndira,
Sé que han pasado muchos años de ésta pregunta y quizás ya tuvo una respuesta. Pero sí es una concha de Mexithauma quadripaludium, no es que sea un morfotipo distinto por ser la concha blanca, lo que pasa es que las bandas en color oscuro que tienen estas conchas es una capa epidérmica que se llama periostraco, el cual suele desprenderse. En el sedimento de las pozas de Cuatro ciénegas hay miles de conchas como las de la fotografía, y son conchas ya vacías. Por lo que podría decirse que al morir el animal el periostraco se va desprendiendo y queda la concha en color blanco.
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I am running this code (below) to add error bars onto my bar chart, i am trying to to work out the standard deviation (sd) of shannons diversity index
The code works up until the second/third last line
geom_errorbar(aes(ymin=shannon-sd, ymax=shannon+sd), width=0.2,
position=position_dodge(0.9))
This error code keeps coming up
"Error in shannon - sd : non-numeric argument to binary operator"
but this is my data...
Exposure genus shannon sd
Exposed Crustacean 0.000000 0.00000000
Exposed Mollusc 1.199625 0.13291129
Exposed Seaweed 1.513125 0.42093822
Sheltered Crustacean 0.025500 0.07212489
Sheltered Mollusc 1.156750 0.26763341
Sheltered Seaweed 1.848125 0.27264128
anyone know where im going wrong?
library(ggplot2)
#+++++++++++++++++++++++++
# Function to calculate the mean and the standard deviation
# for each group
#+++++++++++++++++++++++++
# data : a data frame
# varname : the name of a column containing the variable
#to be summariezed
# groupnames : vector of column names to be used as
# grouping variables
data_summary <- function(data, varname, groupnames){
require(plyr)
summary_func <- function(x, col){
c(mean = mean(x[[col]], na.rm=TRUE),
sd = sd(x[[col]], na.rm=TRUE))
}
data_sum<-ddply(data, groupnames, .fun=summary_func,
varname)
data_sum <- rename(data_sum, c("mean" = varname))
return(data_sum)
}
df3 <- data_summary(diversity, varname="shannon",
groupnames=c("Exposure", "genus"))
# Convert dose to a factor variable
df3$genus=as.factor(df3$genus)
head(df3)
p <- ggplot(diversity, aes(x=genus, y=shannon, fill = location)) +
geom_bar(stat="identity", width = 0.5, position=position_dodge()) + theme_minimal() +
geom_errorbar(aes(ymin=shannon-sd, ymax=shannon+sd), width=0.2,
position=position_dodge(0.9))
p + scale_fill_brewer(palette="Paired") + theme_minimal()
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Could you read the data into a very simple program, possibly multiplying the numbers to see if you get the error because the file is corrupted a bit. You could also move the lines around before running the program to see where it has the problem. As Liang Chen suggests, it seems like a simple problem with the file.
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We have obtained some electron micrographs showing what appears to be developmental stages of some type of small eukaryotic organism colonizing/parasitizing the tissues of mammalian hosts. Samples tested included sterile deep needle aspirate of subcutaneous nodules, filtered lysed whole blood, and urine sediment. The hosts may have a unique genetic defect allowing them to become infected with eukaryotic parasites, or, the putative parasite may have efficient strategies for suppressing the hosts immune response.
We are curious about the identity of this possibly novel organism. None of us in the research group have more than basic knowledge of invertebrate taxonomy, but based on the presence of organized calcified “tube or shell”-like structures, we are hypothesizing that it may be some type of polychaete or mollusk? The opinion or thoughts of anyone skilled in such classification would be very appreciated. Thanks!
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ربما يوجد ذلك
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Fishes. molluscs which are living in salty marine water donot suffer from exo-osmosis why? Are there any defensive mechanism acing against osmotic pressure caused by marine salty water?
Or, the osmotic pressure of fish cell is higher than external water? or there any protective covering covering the body of marine creatures?
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The approximately 22,000 species of fishes alive today live in virtually all sorts of marine and aquatic habitats that are not unduly toxic. Some, including salmon, lampreys, shad, sturgeon and striped bass, move between freshwater bodies and the ocean at least once in their lives to spawn. Many of these anadromous species do so annually, finding conditions needed for reproduction in one realm and those needed for feeding and growth in the other.
These fishes have to switch over their salt balance physiology when they move from fresh to saltwater and back again. They typically make these adjustments in a brackish estuarine environment--which lies on the way between salt water and freshwater habitats.
for further study
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Hi everyone, I am looking for animal data sets that can be compared to the plant datasets from Smithsonian plots. The Smithsonian tree plots census plants in a local community (a plot) above a size threshold; size is measured by stem DBH, and plants are identified to species. I am looking for animal datasets that are similar; that is, they need to contain most of the individuals in a given community (say, mammals or insects or fish) ID'd to species or morphospecies; and they need to have all of the masses of the individuals (not just averages for the species). So far I have found some good small mammal trapping data sets from LTER in the USA, and some tree canopy fumigation datasets for insects. What kind of data are available for large mammals, insects, spiders, molluscs, fish, birds, reptiles, amphibians, etc.? I am looking for datasets of any size, location, and timescale. Thanks so much.
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Valerie Partridge
Thank you, that's exactly the kind of dataset that would be a useful comparison. Are these data available or published?
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The gill of this B. plumula is visible below the raised mantle. In addition there is a tripinnate feature which extends further out onto the area of encrusting pink alga. Can anyone tell me its name and function? Or is it a malformation?
The drawing in Thompson (1976) Biology of Opisthobranch Molluscs V. 1 shows the gill as a simply pinnate plume. Is there a published paper with a detailed dissection of B. plumula?
Please click the image for a clear view of the feature which is partly hidden on the preview.
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It could be a supernumerary gill, a malformation. Where and how is it inserted ?
Nice photo !
Best wishes
Auguste
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Hello all,
I am about to start a project which is to identify a newly isolated bacteria as a causative pathogen to mass summer mortality in a marine mollusk. Briefly, this is a full-factorial design experiment that will involve bacterial infections (control/injection) with temperature treatments (ambient/ elevated), resulting in 9~12 treatment groups consisting of 10 animals. And I plan to sample the animals at different sampling time points (6-time points) which will end up with hundreds of animals (450~500) that need to be sacrificed for blood sampling. In this particular experiment, I am also attempting to determine a bacteria load after the animal challenge test.
Can someone suggest a suitable approach to quantify and detect bacteria in the presence of blood cells since we know if we use qPCR as a bacteria quantification method, it will not differentiate between live and dead bacteria? Another way of doing this based on literature is plate counting (in duplicate, around 3-4 serial dilutions of blood samples per animal) will also not be a doable method, just too many plates to spread, incubate and count, time-consuming and labor-intensive (according to a massive number of samples)?
Thanks for your assistance.
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As Traci Testerman says you should always validate chemical of PCR methods, but you could then use a "live-dead" PCR as described here: Askar M et al. Propidium monoazide–polymerase chain reaction for detection of residual periprosthetic joint infection in two‐stage revision. Mol Biol Reports 2019:46; 6463-70. doi.org/10.1007/s11033-019-05092-z
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My question is about the inconsistency between the species authorship date and the journal publication date that appeared since Zoobank came into use.
I would like to extend my question with the following example:
Szabo et al. published a paper entitled "Gastropods from the Jurassic neptunian sills of Rocca Busambra (north-western Sicily, Italy): Patellogastropoda, Pleurotomarioidea, Scissurelloidea, Fissurelloidea and Eucycloidea" in Papers in Palaeontology. They registered their species to Zoobank in 2019, when the online first view was published. So when I refer a species from their publication, I cite the species as following "Trapanimaria gattoi Szabo et al., 2019". Their paper is included in an issue and printed in 2021. So when I cite their paper, I add the citation in the reference section as following "Szabó, J., Conti, M. A., Monari, S., & Wendt, J. (2021). Gastropods from the Jurassic neptunian sills of Rocca Busambra (north‐western Sicily, Italy): Patellogastropoda, Pleurotomarioidea, Scissurelloidea, Fissurelloidea and Eucycloidea. Papers in Palaeontology, 7(1), 27-110". This creates an inconsistency between the date of species authorship (2019) and the date of end-text reference (2021).
I wonder how other researchers solve this date inconsistency in their manuscripts.
Thanks in advance.
The article can be reached here:
Zoobank link for the publication:
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I wonder if the early view from 2019 meets the requirements of the Code. The new taxa have ZooBank LSIDs and the paper is registrered in ZooBank, but the latter needs to be mentioned explicitly for an electronic publication to be valid in the sense of the Code.
If the electronic version from 2019 complies with the Code, the publication date of the new names is 2019. But how to cite this paper, if the issue number and final pagination wasn't available until 2021? We have the very same problem in our database.
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Dear colleagues!
Have you ever seen a program which is able to select the most suitable pairs of group-specific primers from a set (more than 5 pairs) of proposed ones?
Suppose in my lab I have several pairs of primers for different groups of organisms (polychaetes, fish, mollusks, echinoderms) and I want, instead of ordering new ones, to select from them those that are absolutely accidentally found to be specific to... crustaceans.
In other words, a bulk search among available primers that have never been tested for specificity to a particular group.
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In general, everything can be found on
I did this for different types of beetles. The point is that you need patience, because if there are many records in the database, they must be sorted. On the site you can find sections of genes that have not yet been identified.
Regards, Sergey
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Dear marine biologists,
Thank you in advance for helping me in identifying this Mediterranean limpet. These beautiful limpets were collected from Lebanese rocky shores, Eastern Mediterranean Sea.
I need to know which species is this? Patella caerulea, Patella vulgata, or Patella rustica / lusitanica?
Thanks :)
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I am investigating microplastics in a marine mollusc and would like to obtain an initial particle count by digesting the animal tissue. I am looking into procedures and reaching out to authors with experience in this process for a simple and effective method.
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Although there are many ways to digest organic matter to isolate MPs, strong acids (such as NHO3) are NOT recommended. This is basically because many types of microplastics are susceptible to degradation in contact with this type of acid, showing disintegration, fractioning, decoloring, among other effects.
The most recommended (and cost-efficient) digestion reagents from my point of view are KOH and H2O2.
You can check this paper by Dehaut et al. (2016):
Regards
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All species collected from (subtidal & intertidal) of Qatari waters of Arabian Gulf, ( the size of the species between 5mm -10 cm long and between 5mm -5 cm wide). All the pictures were taken by me.
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Can any one help me to confirm the Mollusks species names or find out the names of the following species?
All species collected from (subtidal & intertidal) of Qatari waters of Arabian Gulf, ( the size of the species between 5mm -10 cm long and between 5mm -5 cm wide). All the pictures were taken by me.
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At some bays, a decrease in dissolved oxygen is observed in the natural habitat of molluscs and other benthic invertebrates. I assume that there is a good correlation between the reduction of dissolved oxygen and the volume of organic matter contained in waste and effluents from anthropogenic activities.
I share benefit from your opinions.
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La consommation d'oxygène est la manifestation la plus évidente de l'activité de dégradation de la matière organique par les bactéries dans les milieux naturels. Par temps de pluie, les surverses de réseau unitaire dans le milieu récepteur peuvent conduire à des désoxygénations particulièrement intenses, voire à l'anoxie complète du milieu à certains endroits, provoquant ainsi de fortes mortalités piscicoles […..]
Cependant, d'importants aspects du fonctionnement des écosystèmes aquatiques sont basés sur le cycle de la matière organique (M.O.) qui est étroitement couplé à celui de l'oxygène dissous. Dans les milieux aquatiques où les apports allochtones de matières organiques se font en quantité limitée, la consommation d'oxygène résultant de la biodégradation de la M.O. par les bacté¬ries hétérotrophes est aisément compensée par la production d'oxygène des processus photo¬synthétiques et la réaération du milieu ; il en résulte un bilan d'oxygène équilibré de l'éco¬système. Lorsque, à l'opposé, des charges importantes de M.O. sont apportées au milieu, par exemple via des rejets ponctuels, les processus d'apport d'oxygène au milieu peuvent s'avérer insuffisants pour compenser la consommation bactérienne d'oxygène et des problèmes de sous-oxygénation du milieu peuvent apparaître.
Matière organique dans les milieux naturels. www.https://hal.archives-ouvertes.fr
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I read some articles on mollusc and fish diet analysis. The formula for percentage frequency of occurrence stated above:
%F0 = Nei/Ne x 100
and percentage numerical abundance :
%NI : Ni/N x 100
especially in mollusc. I want to know how is the calculation for Nei, Ni and N take place, is it an individual organisms count under microscope slide or do we need to used specific device or equipments such as haemocytometer or sedgewick rafter to count the number of the cell per area before we can fill in the Nei, Ni and N in the formula?
Thank you.
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If you are counting food items, then both formulas will give you exactly the same result. Now, the real catch is what we mean when we say abundance. Is it the amount of events of finding a given food item? Is it how much biomass of a given food item a mollusc is ingesting? Is it a standardized methodology of counting? This is something that you have to think about.
Every time you want to find out how much of something is ingested we need to think about this: A count is meaningless in its nature. The count is only meaningful if you scale it to the representation of the total. Here is where the percentage comes into play. It will always capture the proportional amount of something. Therefore, we will always do it as: part/total X 100.
Now for your methods, I don't know much about molluscs but perhaps you could put the contents of the crop into a slide. The slide is now your sample. The Total is the total count of food items. The parts are the frequency of a given item. The same logic apply if you decide to quantify abundance using biomass (which is perhaps better but way more difficult to do), but the counts will be replaced with weights.
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I and my coauthor have recently completed a paper on the mollusks found in a shell mound in Marin County, northern California. We're both paleontologists and are looking for someone with experience in California archaeology to review our paper. We are not plugged into the archaeology community and can surely benefit from expertise in that discipline. Thanks Chuck Powell.
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Hello Chuck; Matt is the son of a cousin (I never know what to call that relationship). He works at Calif. State Univ. Northridge. His email address there is... matthew.deslauriers@csusb.edu
I see that he has an odd address. Regards, Jim Des Lauriers
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Hi all,
found unusually small razor clams from bottom set gill nets. The valves are elongated and the shell is fragile. Sizes are less than 7 cm in length and 0.9 cm width. Can anyone confirm the species of this Solen?
Best
Deepak
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Dear sir,
It is Solen linearis Spengler, 1794.
It is lengthy and narrow in width. Compared to other Solen species, it is quite narrow.
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Whoever has worked on abalone...
I have been trying to extract DNA from the blackfoot abalone (attachment muscle) and the amount of the extract is very low, no matter how much Proteinase K I add, the amount of tissue I use and the final elution buffer. Is there anyone out there who has worked on abalone who has a working protocol to use ?
Thanks
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In case you have not solved your problem yet I have a few thoughts.
Disclaimer, I have not worked on abalone.
I will say a few things I have learned from experience with mixed aquatic invertebrate DNA extractions.
1) There are kits specifically formulated for the challenges of molluscs (e.g. Omega EZNA Mollusc DNA extraction kit). If you cannot use the kit for whatever reason, consider looking at the protocol and reagents to figure out why your current protocol might not be working well.
2) Are you also using RNAse in the extraction protocol? Both Proteinase K and RNAse A are used in most protocols I use, including the mollusc kit noted above.
3) If you are doing a silica-based spin column extraction, be careful not to overload the column with material. It can result in lower and/or poor quality DNA extract. Most kits have maximum recommended amounts of tissue for this reason.
4) Also for spin-column protocols: Multiple repeated elutions, extending the time you incubate your elution solution on the silica column, using warm elution buffer, can also help you recover more total DNA.
Good luck!
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Full history is in the project description, but briefly- a team of other veterinarians/colleagues and myself have stumbled across a case of multiple animals that have similar symptoms and are co-housed. Their presentation suggests an infectious disease, but no known infectious agent could be found despite thorough work-up. However, cytology samples from clean aspirates of subcutaneous nodules and urine filtrate (they have bloody urine) seems to be showing repeated structures that do not appear mammalian in origin. We seek to understand if these structures are some type of very organized artifact, or if they instead suggest that there may be some very unusual/novel type of organism (Annelid or Polychaete esp.) causing/involved-in these animals illnesses. Thank you for your time. Invertebrate Taxonomy Parasites Mollusca Polychaete taxonomy Annelida
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The structures shown in the attached photos appears to be artefacts. Moreover, no annelid group is adapted to parasitism in subcutaneous nodules .
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This type of slug abounds in my garden, located at 2,350 meters above sea level in the Andes of northern Peru (Chachapoyas, Amazonas department). Any idea to which family and genus it belong?
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Hamed Ghanaatian - they are somewhat smaller - 3 to 4 cm.
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Dear Marine Biologists,
Do you have any experience in collecting plasma of abalone that you could give me some advice? I see 2 different methods from publication:
· cut adductor muscle in each animal with a scalpel, and collect hemolymph from the blood sinus (Zhou et al., 2015)
· collect from the pedal sinus using a 1 ml syringe with a 27-gauge needle (Venter et al., 2018a).
Which method is better? If we cut the adductor muscle, hemolymph may get contaminated with mucus?
Many thanks.
Thao
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For example,
"Taxonomy and Systematics of Molluscs"
or "Systematics of Molluscs"
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Nipu, since the definition of systematics as the systematic classification of organisms (i.e., taxonomy) and their evolutionary relationships encompasses both taxonomy and phylogeny then using "systematics of molluscs" alone would be fine. However, using both taxonomy and systematics would be acceptable by some as well.
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Full history is in the project description, but briefly- a team of other veterinarians/colleagues and myself have stumbled across a case of multiple animals that have similar symptoms and are co-housed. Their presentation suggests an infectious disease, but no known infectious agent could be found despite thorough work-up. However, cytology samples from clean aspirates of subcutaneous nodules and urine filtrate (they have bloody urine) seems to be showing repeated structures that do not appear mammalian in origin. We seek to understand if these structures are some type of very organized artifact, or if they instead suggest that there may be some very unusual/novel type of organism (Annelid or Polychaete esp.) causing/involved-in these animals illnesses. Thank you for your time.
Invertebrate Taxonomy Parasites Mollusca Polychaete taxonomy
Annelida
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Hi Peter, that is good to know, thank you. I will look into it.
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I am looking for a pdf versión of the Part K. MOLLUSCA 3 (Cephalopoda General Features, Endoceratoidea, Actinoceratoidea, Nautiloidea, Bactritoidea) Treatise on invertebrate paleontology.
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Dear Daniel,
Here you go!
Best wishes, Thomas
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I work in the Institute of Zoology Academy of Sciences of Moldova. My main activities are: collecting, handling and determination of freshwater macroinvertebrate up to species level (Mollusca, Crustacea, Ephemeroptera, Trichoptera, Chironomidae) according to the standardized hydrobiological methods. I have diferent species in collection, the preservation of the samples has been made by adding of 70-96% ethyl alcohol. I use of SteREO Discovery.V8 (Zeiss) and upright microscope Axio Imager А.2 (Zeiss) and ZEN modules: Measurement, Extended Focus , Time Lapse. I have a theoretical experience in PCR and DNA sequence analysis.
I would be interested to participate in the STSM COST Action: CA15219 and would like to ask you, if anybody is interested to collaborate with me as a Host professor?
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Hi Oxana,
my lab is working a lot with DNA barcoding and phylogeography of freshwater macroinvertebrates from all over Europe, particularly from the south-eastern part. We are very open to collaboration. We're also part of DNAqua-Net (CA15219 ) Please contact me at priv.
Best,
Michal
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Hi Everyone,
Does anyone have experience in working with haemocytes of P. vannamei or P. monodon? Could you please suggest me any effective method to prevent cell clumping when taking haemolymph out from shrimp?
Thank you so much.
Thao
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This is not for your species, but should help. Addition of some EDTA calcium/magnesium chelator, adjusted to neutral (pH7) or haemolyph pH should work. Add 1-2 drops of a 2% solution per ml. If the clumping occurs as you harvest the haemolymph, try starting with a small amount of 0.5% EDTA or EGTA in saline in the syringe or pipette You use to collect your sample.
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I am planning some fieldwork in Algeria to assess the conservation status of freshwater bivalves (Unionidae), I can only find old 19th century published records, and it would help me to plan the field work.
I will be thankful for any eventual records you might have.
Kind Regards
Manuel
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Freshwater Mussels - College of the Environment and Life ...
cels.uri.edu › docslink › water-quality-factsheets › Mussels_Updated
by M Hunt
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Dear colleagues,
Could someone refer me a researcher who can identify this species of parasite in freshwater mollusks?
We have some specimens and can to sent for analysis if necessary.
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Hello can i help you send me samples of their photos given my research on freshwater snails
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Dear colleagues,
I would like to join the group. My experience is largest on Mediterranean species, as well Iberian and Macaronesian taxa.
In the past I have asked several times to join the IUCn Mollusc Specialist Group, without answer. I think my experience could add something.
All the best,
Cristian R. Altaba
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Cristian,
I think you can just add yourself as a follower of the project. That it is in my name is rather an accident, as I am only peripherally involved. Eike Neubert is the one most heavily involved in managing the work.
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I am looking for articles dealing with the calcium uptake in marine mollusks such as cephalopods, bivalves or gastropods. Specifically, whether the Ca comes from sea water or from food sources.
Thank you in advance!
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A few classic papers that should be useful (PDFs attached):
1. Van Der Borght & Van Puymbroeck (1966) Calcium metabolism in a freshwater mollusc quantitative importance of water and food as supply for calcium during growth. Nature 210:791-793.
From the conclusions: "Even when food is offered ad libitum and when the period of observation is relatively long (2.5 months versus the life-cycle of 4 months from egg to egg in laboratory conditions), about 80 percent of the calcium fixed by the animals is derived directly from the water."
2. Bevelander (1952) Calcification in molluscs. lll. Intake and deposition of the Ca45 and P32 in relation to shell formation. Biol. Bull. 102:9-15.
(...and references therein).
From the Introduction: "In regard to the source of calcium which is utilized in the formation of the shell, Orton (1925) observed that shells continue to grow in the English oyster in the absence of food. Similar observations were recorded for the American oyster by Galtsoff (1934) who states in this connection that the amount of calcium utilized is many times greater than could be stored in tissues."
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I want to need some information regarding those selected molluscs families for the geographic distributional range from Indian to Pacific Ocean.
I mainly focus on Marine Molluscs of Class Bivalvia, Gastropoda and Polyplacophora respectively.
Thanks !
Kind regards, Saedul
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For bivalves, a lot of distributional data were summarixed by Huber in two books (2010; 2015), I guess, you know them. There are xls files on CDs supplemented both books with distributio of all bivalve species known up to date.
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Do anybody have "Monograph of Living Chitons (Mollusca: Polyplacophora)" by Piet Kaas and Richard A.Van Belle
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Thank you Dmitry Telnov , Godfried Van Moorsel , Shahnawaz Gul and Saedul Bibi . The books in google book does not have access to all the pages.
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please I want to know the best method or protocol for DNA extraction for animals (molluscs, leeches, turtles, fish, snakes) ?
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I have attached several articles on DNA extraction in fish .
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Does anyone know first-hand or know of a recorded observation as to what hemocyanin (the oxygen-binding molecule in the blood of most arthropods and mollusks) smells/tastes like? I know that hemocyanin is copper-based, but in some respects does not resemble copper visually (blue of green) due to the surrounding molecule. I know that vertebrate blood is often described as having a coppery taste despite being iron-based, so I was wondering what hemocyanin would taste like. Does it taste anything like the taste of crab, squid, or other hemocyanin-using organisms?
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Hello, similar topic well discussed here.
Good luck!
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I have 195 gastropod (Buccinum undatum) samples that have been extracted using the phenol-chloroform method and the same 195 extracted using the ENZA Mollusc DNA extraction Kit. Individually, I have had varying success with the methods. However, when combined I can choose 195 good High Molecular weight extractions that are suitable.
I will be doing library preperation using 195 samples for ddRAD (ApeKI and BamHI) and I am wondering if it is alright to use DNA extracted via different methods to construct a single library?
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Declan Morrissey there is no problem, you can use the samples you got the best extractions results.
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I am extracting DNA from Gastropod tissue using the E.Z.N.A Mollusc DNA kit. However, I am waiting for the kit to arrive and I was wiondering if anyone knows Can I homogenise samples using Liquid Nitrogen and then store them (7 days max) before adding them to the ML1 buffer and incubating?
If this does not affect the quality of genomic DNA then it will save me alot of time.
I have attached the EZNA Mollusc kit protocol
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I think you could store your tissue in liquid nitrogen for rapid freezing then transfer it into -80℃ or just keep tissue in liquid nitrogen before you extract, it is okay to store in liquid nitrogen in short time.
I am not sure about the quality of homogenise samples which are pretrement. I always grind tissue then I extract DNA and my tissue samples are stored at -80℃ all the time (I am extracting genomic DNA of Echinoderms).
I hope it will help you.
Good luck for genomic DNA extraction!
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I encountered this gastropod in sediment taken by a bottom grab on the summit of the Great Meteor Seamount (NE Atlantic) in about 300 m depth. The figure is from a sub-adult, adult specimens have one more whorl and reach a height of about 2.5 mm.
Please note the unusual protoconch with reticulated sculpture and the concavely curved lip.
I would be happy with a family that would fit.
Answer: Luigi Romani provided the correct name Haloceras meteoricum Gofas 2018
Leon Hoffman
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It is very similar to (and probably is) Haloceras meteoricum Gofas, 2018
see: Gofas S. (2018). A non-planktotrophic haloceratid (Gastropoda) from the Meteor seamount group, central North Atlantic. Iberus. 36(2): 149-155.
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I am currently conducting a meta-analysis on the lipid profiles of mollusks fed different algae diets (with distinct lipid profiles). I am looking for any relevant articles or reports, specifically with data for the different fatty acids detected in mollusks (i.e., from fatty acid analysis) and any mention of their algae diets (i.e., the type of algae).
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Dear Babaran,
Actually I do not have any information about the lipid profiles of mollusks on different algae diets but I have some fatty acids profile data of mollusks. Plz see the attached documents.
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Currently working on marine macro-invertebrates (especially molluscs, echinoderms and crustaceans) from gleaners' catch for my thesis and need help in taxonomic identification and confirmation. Thank you.
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@ Jyl C Marfil : You are welcome
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W. Kobelt (1881) in Catalog der im europäischen Faunengebiet lebenden Binnenconchylien used abbreviated references to earlier literature. What could mean abbreviations "Kstr." and "Mon. Alb."? Bot h obviously refer to works with many illustrations (see the attached sample pge).
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I think "Mon. Alb" is O. Boettger, 1878; Monographie der Clausiliensection Albinaria v. Vest. In: L. Pfeiffer: Novitates Conchologicae 5. Cassel. Pp. 39-173, pls. 145-148 (available on line).
Boettger described Albinaria discolor inaequata based on material from Blanc's collection with "MS" (manuscript) mention of inaequata on his label (see page 130).
"Kstr." is indeed a Kuster's monograph as indicated by Maxim. It is H.C. Küster, 1847; Die Schliessschnecken und die verwandten Gattungen (Clausilia, Balea, Cylindrella, Megaspira) In: Abbildungen nach der Natur mit Beschreibungen. Systematisches Conchylien-Cabinet von Martini und Chemnitz 1 (14): 1-355, pls. 1-38. Clausilia discolor is p. 80-81, n° 71; pl. 8, figs. 34-37.
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I have found numerous eggs on the lower side of stone in fast flowing river section (Latvia, geographically central part of the Europe). What species lay these eggs? Ancylus fluviatilis (Mollusca)? Other mollusc? Caddishflies (Trichoptera)? Something else?
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I agree that they don't look like molluscs. Can you collect some and watch as they develop?
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This ovigerous mass was 4 meters deep, on a soft seabed but also near hard seabed, probably produced by a gastropod mollusk, can anyone tell me if there is an atlas of the eggsmass of the gastropods?
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Thanks to everyone for the suggestion, the picture was taken in Ionian Sea, near Santa Maria di Leuca
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Dear All,
Here is the key facts of world fisheries and aquaculture status 2018 by FAO.
---------------------------------
Global aquaculture production in 2016 was 110.2 million tonnes with the total farm-gate value estimated at USD 243.5 billion.
The total production included 80.0 million tonnes of food fish, 30.1 million tonnes of aquatic algae and 37 900 tonnes of ornamental shells and pearls.
The contribution of aquaculture to the global fish production has reached 46.8 percent in 2016, up from 25.7 percent in 2000.
In 2016, there were 37 countries, homes to close to half of the human population in the world, producing more fish from aquaculture than wild capture.
Of the 80 million tonnes of farmed food fish production in 2016, inland aquaculture contributed 64.2 percent (51.4 million tonnes), while marine and coastal aquaculture counted for 35.8 percent (28.7 million tonnes).
Inland aquaculture is dominated by finfish species (92.5 percent, 47.5 million tonnes), followed distantly by crustacean (5.9 percent, 3.0 million tonnes).
Marine and coastal aquaculture relied heavily on molluscs farming (58.8 percent, 16.9 million tonnes), with finfish (6.6 million tonnes) and crustaceans (4.8 million tonnes) counting for 22.9 percent and 16.8 percent, respectively.
Information: X. Zhou (FAO)
Please see the full report in the attachment. Many thanks.
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Many thanks for sharing this - dan
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To be specific, how to buy angle wings (a bivalve mollusk) in US?
Thanks.
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Kenneth M Towe
I did contacted the museum but they don't sell them to us. But anyway... thank you so much, Kenneth.
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Can anybody help me with this freshwater snail? It was collected on 25.ix.2018 in a Ditch at Papenveer, The Netherlands by Nick Kroese (lat 52.186 lon 4.722) In The Netherlands we only have Physella acuta (=heterostropha) but this one is untypical. There is a garden centre nearby, so maybe a non-indigenous one? Any suggestions? It is photographed on frogbit Hydrocharis morsus-ranae.
Many thanks in advance
Ton van Haaren
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Ok!
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I am looking for information about the feeding guilds, motility and distribution of megabenthic fauna, I found the mollusks information in https://porites.geology.uiowa.edu/database/mollusc/mollusclifestyles.htm, but I need to store information of Crustaceans, Echinoderms, Poriferans and Cnidarians.
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Dear Maria
Thank you for your help
Have a nice week.
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Currently, I am trying to determine the population genetic structure of two species of nudibranchs from the Caribbean. Previous research has been done with tradicional molecular methods and this is our fist attempt using NGS. So, I would like know if there is a good services provider in order to get the RAD-seq data for our project. DNA extraction is the only step that we're going to carry out in our lab.
Thanks!
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Hi Kimberly, I just sent you a message. Feel free to email me.
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Ocean acidification is the lowering of ocean pH due to increasing levels of CO2 in the atmosphere (from fossil fuel burning, deforestation etc.). The absorption of CO2 has already acidified the surface layers of the ocean causing an overall decrease of 0.1 pH units since the pre-industrial period, which is equivalent to a 30% increase in acidity and a 16% decrease in carbonate ion concentrations. The surface ocean pH is projected to decrease by 0.3-0.4 pH units by 2100 (predicted to decline from approximately 8.2 in pre-industrial times to 7.8 by the end of this century). The changes in basic ocean chemistry due to ocean acidification are likely to have impacts on organisms that require calcium carbonate to build their shells or skeletons such as corals, and molluscs (oysters, mussels, pteropods, and abalone). There are three naturally occurring forms of calcium carbonate used by marine organisms to build shells, plates or skeletons: calcite (e.g. marine plankton coccolithophores), aragonite (e.g. corals, pteropods) and high magnesium calcite (e.g. starfish, sea urchins, brittle stars). The solubility & sensitivity to ocean acidification is higher with magnesium calcite and the least with calcite in the following order: magnesium calcite>aragonite> calcite.
Increasing ocean acidification can significantly reduce the ability of reef-building corals to produce their skeletons via reduced calcification. Successful fertilisation, larval settlement, recruitment, growth and survivorship of corals can be affected due to ocean acidification. A recent research shows that corals, echinoderms and molluscs are very sensitive to a decline in the pH value compared to crustaceans (Wittmann and Pörtner 2013). Many marine fish (about 25% of known marine fish) use coral reef as a habitat, shelter (refuge) and food. Coral reefs provide food and livelihood security for some 500 million people worldwide including 90% protein need of inhabitants of Pacific Island Developing Nations. Coral reefs are the primary economic driver in many tourist destinations and protect fragile coastlines from threats such as tsunamis and erosions.
Some experimental results showed that calcification is generally reduced in mussels under near-future CO2 levels. Projected future CO2 level (rise of ocean acidification) can impact on shell formation, larval development, and survival rate in abalone. A study on the early development of the oyster (Crassostrea gigas) found that shell calcification is reduced in juveniles and their body shape and size are also altered. Many mollusc species at the adult and juvenile stages have shown reduced growth and/or health under projected ocean acidification scenarios. Molluscs are food for commercial fish such as haddock, halibut, herring, flounder and cod. Clams, scallops, mussels, oysters, abalone and conchs provide direct protein sources for various island and coastal communities and are valuable commercial fisheries. Molluscs account for 8% of the global marine catch.
Though the effects of increased acidity on adult finfish seems to be minimal or supposed to be largely unaffected (since fish are able to control their acid-base balance by bicarbonate buffering, mainly across the gills and via the kidney), however, some recent experiments with tropical coral reef fish suggest that the sensory systems of fish can be affected by ocean acidification. For example, when clownfish (Amphiprion percula) were exposed to higher CO2 levels, they could not distinguish predator from non-predator and were found swimming toward predators, instead of away from them (Dixson et al. 2010). The loss of the senses of sight/smell/touch due to ocean acidification would thus reduce survival in commercially important fish species. Another experiment (Frommell et al. 2012) showed detrimental effects of ocean acidification on the developmental stages of Atlantic cod larvae (Gadus morhua). Exposure to elevated CO2 levels resulted in severe to lethal tissue damage in many internal organs in larval cod, with the degree of damage increasing with CO2 concentrations. As larval survival is the bottleneck to recruitment, ocean acidification has the potential to act as an additional source of natural mortality, which may affect populations of already exploited fish stocks. A small change in early life survival can generate large fluctuations in adult-fish abundance in the wild.
Antarctic krill (Euphausia spp.) is a key pelagic species in the southern region and represents the largest fishery resource. Many animals like whales, seals, penguins and fish are dependent on krill fishery. Marine ecosystems in particular krill populations could be vulnerable to ocean acidification. For example, when krill eggs were exposed to elevated seawater CO2 levels, hatch rates were found significantly lower, it also delayed embryonic development (Kawaguchi et al. 2013). The pteropod, or “sea butterfly” (with aragonite shells) are an important food source (for fish such as juvenile salmon, birds, tiny krill, and giant whales). They (pteropods) are also a good indicator of ecosystem health and play an important role in the oceanic carbon cycle. The shells of pteropods, Limacina helicina antarctica – living in the seas around Antarctica are being severely dissolved by ocean acidification according to a new study (Bednaršek et al. 2012). The main consequence of loss of shell due to ocean acidification will be increasing vulnerability of pteropods to predation and infection, which will in turn impact other parts of the food web.
Ocean acidification may cause an increase in jellyfish (Attrill et al. 2008). Jellyfish are key predators and can affect the abundance of zooplankton, fish larvae and eggs, which affects survival to the adult stage (or recruitment) of fish populations. As jellyfish are rarely the preferred food for other marine animals, any significant increase in their numbers could have major consequences for pelagic ecosystems and fisheries.
Nevertheless, rising CO2 may enhance productivity of non calcifying seagrasses, seaweeds as they require CO2 for photosynthesis and living, for example, photosynthetic organisms such as seagrasses showed higher growth rates, as much as five-fold or higher with acidification (Hendriks et al.2010 )
Question: Will ocean acidification be a threat to seafood security, commercial fishing and livelihoods? If so, how?
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The daily activities of human beings result in the emission of huge amounts of harmful gases, such as carbon dioxide, sulfur oxides and nitrogen. The most important of these activities are the various industries based on burning fuel, power plants, heavy fuel, transport,
 These gases are released into the atmosphere, many of which dissolve in rainwater and sea and ocean waters. Carbon dioxide is dissolved in water and carbonic acid is formed, which causes the acidity of the water to rise.
Many marine organisms are affected by the acidification of the oceans. Many marine systems have deteriorated in many regions of the world after the concentration of carbon dioxide in the atmosphere has increased significantly,
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Does anyone have an opinion on my attempt at ID?
Having accessed Australian Museum records and some online imagery - I have come up with a species ID of
Nototriphora vestita -
(or perhaps a closely related but undescribed species)
Shown here are adults and 2 juveniles
These were collected from the intertidal zone of a rocky beach near Adelaide in South Australia
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I'm not sure Bittium occurs in S Australia. Maybe Cacozeliana icarus?
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Dear Research Gate.
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Hello Dr. Blanca Figuerola, Dr. Tomas Tomascik and Dr. Paolo Albano,
Thank you for the wonderful answers to our question. Ok, we will contact Prof Suzuki, check out Potamididae/ Muricidae/ Thais lacera, Dr. Albano's molluscs books and get back to you on the hinge photo. Thank you.
Cheers,
Kwong Kok Onn.
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Help me to identify this Bivalve .collected from Gulf of Mannar India. I identified this specimen till genus level Meretrix, Is it Meretrix petechialis (Lamarck,1818)?
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Again Meretrix casta for sure!
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I am sorting mollusks from coral habitats (sediment samples in 450 - 600 m water depth) off the Great Bahama Bank, off western Florida and off northern Yucatan. I struggle with a large number of unknown species as I am unfamiliar with the region and do not have access to many historical papers with imaged species. I am looking for an authority from the region who would be willing to help out. The molluscan material looks very promising in terms of rare and unknown species with potential for publication in 2019-2020.
For more information, please refer to my update on the project on NW Atlantic Mollusca.
Grateful for any help, Leon Hoffman
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Dear Leon,
Will you be posting photos? I can try. I have many books and reprints to access. It is just a matter of seeing what you are trying to identify
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Our lab has observed this feature on presumed naticid drill holes penetrating shells of Saxolucina (Megaxinus) anodonta (Say) from the Miocene Choptank Formation, Patuxent River, Maryland, USA.
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I find this highly questionable because neither naticids nor muricids are able to produce nacre! They build their shells with a crossed lamellar shell structure. Even stranger, also the lucinid that seems to have been drilled wouldn't be able to secrete nacreous shell, because also lucinids have crossed lamellar shell structure. Is you identification of the nacre based on SEM observations?
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This bivalve looks like Cardita senegalensis based on the elongaeted shell on the posterior side of the shell. It also has a close resemblance to C. calyculata which has a shorter extension. Elevated blunt thorn-like structures are noticed in the ribs with mild orange dots. 
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geographical spread suggests Cardita leana but the shape is very similar to Cardita caliculata which could have been introduced by the Red Sea. The specimen is very similar to one that i find in Mediterranean Sea.
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I have not found enough in literature.
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Hello the only reference I find is the following in my database.
Morton, B. (1979). A comparison of lip structure and function correlated with other aspects of the functional morphology of Lima lima, Limaria (Platilimaria) fragilis, and Limaria (Platilimaria) hongkongensis sp. nov.(Bivalvia: Limacea). Canadian Journal of Zoology, 57(4), 728-742.
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This ovigerous mass was found 10 meters deep, in the Tyrrhenian Sea, near the Argentario Mount, but it was also found in the harbour of Trieste in 2009. Which invertebrate could have produced it?
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T you very much Godfried
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I have two possible gene length for the protein-coding gene cob, in a mollusc species: one of the two lengths is longer with 9 nucleotides (= 3 amino acids) more at the beginning. Both gene lengths are biologically possible: proper start codon, and gene length in the range of what is found in other related species. It is not possible to make a choice between the two possible gene lengths based on the primary DNA sequence only, and to know eventually which annotation is correct.
Therefore, I would like to compare the secondary structure and the tertiary structure of the two putative proteins encoded by the gene, in order to find clues that could help me distinguish the right gene length from the wrong one.
Does anyone could recommend a program to infer secondary and/or tertiary protein structure, and a strategy to eventually pick the right gene annotation?
NB: my mollusc species is not a model organism and is not present in databases of such kind of structures like PDB.
Thank you to all,
Severine
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Your statements are all correct, it is certainly true that earlier annotations end up influencing or biasing the later annotations. It is quite possible that most are mis-annotated if the early ones were mis-annotated. For most purposes such as phylogenetic analysis of the relationships or shared ancestries of the organisms, it won't matter if the start codon is correct or not. It is very difficult to isolate the protein product of the gene and sequence the protein to determine the amino acids at the amino-terminal end of the protein, so that is rarely done.
If you annotate your gene to be most similar to the way the others are annotated, at least thee is some consistency, right or wrong. If you annotate it differently, some people may assume you have solid experimental basis for that. You can add a "note" to the annotation saying you chose this start codon because it was most like the annotations done for other genomes, and not give the impression that there is solid experimental evidence for any of them.
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Hi all,
I am looking for help in identifying a gastropod from an archaeological site in the Gobi Desert in southern Mongolia. The site is centred around a small palaeolake. In the middle is a yardang with these little shells. Found in association with the shells, was a Late Neolithic/Eneolithic (6-5 kyr cal. B.P.) stone blade. I've attached several pictures. At the time we didn't measure them, but they are around 6-8mm. I've also attached a photo of some older animals found lower in the yardang stratigraphy on the off chance someone knows them, but they seem to be pre-Holocene. This is for my dissertation, so if anyone has an answer, I will be sure to give you credit! Thank you.
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Ciao,
I agree with Dmitry, they look like Lymnaeidae or something close. Also I suggest that you collect shells and a bulk sample for sieving.
Saluti
Vittorio
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Marine, Tropical
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Fig A: Anodontia sp.
Fig B: Timoclea sp.
Fig. C: Mytilidae
Fig. D: Arcidae???
Fig. E: sinistral Diaphanidae/Philinidae???
Fig. F: Ostreidae
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Sampled from shallow brackish environment. Salinity 14. I suspect the one in the center as abalone shell because it has hole. Please confirm. Thank you.
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For what it is worth, I concur with the earlier opinions. 1) Not an abalone, drill holes on that shell, 2) the shells resemble Ostreidae but are quite worn. Further identification would be difficult.
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what is the species.it is a Linatella
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The low spire of this Monoplex parthenopeus, remember me the more eastern form "echo" from Taiwan and Japan. I have a similar specimen from Masqat (Oman).
Cordially, Gianluigi.
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Hi all, 
I am in the midst of extracting my snail tissue samples via Omega BioTek's EZNA Mollusc specific DNA extraction kit and I am having some shearing/degradation.
I extracted two samples as a test run and they produced a nice, clean band but every sample after I have gotten varying levels of shearing. I have been informed that the DNA is fine for downstream application but this is more for my own sanity...I really don't see what I am doing differently between the extractions. Have I introduced a small amount of DNase contamination? There are vigorous vortexing steps necessary to the removing of contaminants but I did those for all extractions I have done, including the first two. 
All the best!
Brenna
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Thank you to both of you! Sorry for the delay in my response...lab work got put down for a few days as I've been getting in the groove of TAing this spring. 
I think our running buffer is at the end of its life (been re-used close to 15X) and that our pipette is actually pipetting more than 7uL. The wells should be able to handle up to 10uL and I have run that ladder at 7uL previously and hadn't had the same issues. 
Pipettes are being calibrated and I will make fresh running buffer. I am not too worried since I think my DNA is actually fine and at in end of the day that's what matters. 
Thanks everyone!
Brenna
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I'm looking for the species identity.
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Thank you all
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For its Peronia verruculata (Cuvier, 1830), asking for expert suggestions.
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Agree with the above. It is Peronia verrucluata. Have collected specimen during low tides from the Gulf of Kutch. 
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These 4 specimens were found in the southern littoral region or Paraná state, in southern Brazil. They were collected on the banks of a channeled river that runs through the city, close to its estuary.
It is very likely a exotic species, but we have yet to nail down its identity. Could anyone offer a hand?
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Since we have seen Recluzia rolandiana Petit de la Saussaye, 1853 on the beach in NE Florida, USA (Lee, 2009: 98; sp.469), I once looked up some of the more obscure named congeners. Here are some of my notes:
Petit de la Saussaye, S., 1853. Description d'un genre nouveau, G. Recluzia appartenant à la famille des Janthinidées. Journal de Conchyliologie 4: 116-120. <http://www.biodiversitylibrary.org/item/53861#page/122/mode/1up>:
R. jehennei Petit de la Saussaye, 1853: 118. Arabian Gulf. pl. 5, fig. 3.
R. rollandiana Petit de la Saussaye, 1853: 119. Mazatlan, (Pacific) Mexico. pl. 5, fig. 12.
Lymnaea palmeri (Dall, 1871: 135 <http://biodiversitylibrary.org/page/15918288>; not figured) Taqui River, near Guaymas, (Pacific) Mexico.
Dall, W.H.,1871. Descriptions of sixty new forms of mollusks from the west coast of North America and the north Pacific Ocean, with notes on others already described. American Journal of Conchology 7: 93-160, pls. 13-16. 2 Nov. <http://biodiversitylibrary.org/page/15918246>.
I have attached some images (New South Wales, Australia; Texas, USA, and iconotypes of the Petit taxa). All of this tends to support Junn Kitt Foon's characterizarion of the taxonomy of the genus.
Lee, H.G., 2009. Marine Shells of Northeast Florida. Jacksonville Shell Club, Inc. 204 pp. + 19 color plates. 28 May.
Harry G. Lee
Jacksonville, FL, USA
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Is there any record of freshwater gastropod Campeloma sp. (family viviparidae)from Indian subcontinent? Or is it exclusively from American continent?
RAMAKRISHNA AND DEY: Handbook on Indian Freshwater Molluscs
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Water accommodated fractions of diesel oil are highly volatile and insoluble with water. I have to conduct acute and chronic toxicity testing for shrimps, mollusca and fishes in juvenile stages by adopting flow through methodology. 
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Dear Dr Vasanth,
You need to establish some more boundary parameters before the experimental set-up is constructed. (see example of set-up) Journal of Environmental Protection
Vol. 4 No. 7A (2013) , Article ID: 34701 , 7 pages DOI:10.4236/jep.2013.47A008).
It is important to understand the water and the diesel.  Do you plan to feed you test organisms?
1) What sort of water? Fresh, Brackish, Sea? exposure of some materials to 30 days of saltwater could cause problems
2) Diesel comes in grades -(https://www.dieselnet.com/standards/in/fuel.php)   the water soluble portions differ from grade to grade and by how the refining process was done - this can impact volatility which has implications on health and safety in the preparation of materials (and possible contamination of the lab).  You would need to know what sort of diesel you are using and to characterize it for scientific rigor.
Please see the following sites for more information
Kind regards and good luck
Prof Chris Gordon
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1.Which one is male/female crustacean?
2. Name of this young bivalve 
The following species are associated with an ascidian species (colony)?
Please help me 
Regards, 
Durga
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The isopod is some Sphaeromatidae species. An almost similar question in researchgate recently with interesting answers
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Ostrea sp. 
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Tropical Marine benthos. size range 0.5-3mm
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this is my opinion respect to the species regarding your question.
1. Columbellidae (Mitrella sp.) 
2. Mitridae (Mitra sp.)
3. young of Sabia conica (Schumacher)
4. Nassariidae
5. Spondylus (maybe regius)
6. Rissoidae (genus Rissoina)
7. Arcidae
8. Olividae very young
9. Mitridae
10. Columbellidae (genus Mitrella)
Best regards Roberto Ardovini.
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Tropical marine benthos. size range 0.5-3cm
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I suppose Philippines (Visayas). Now i show you the list.
1. Veneridae
2-3 Phasianellidae
4. Marginellidae (genus Volvarina)
5. Rissoidae (genus Rissoina)
6. Mytilidae (genus Brachidontes)
7. Mitrolumna sp
8. Neritidae
9. Muricidae (genus Thais)
10. Mytilidae (genus Mytilaster)
Best regard Roberto Ardovini.
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Hello
Could you help me identify this Isognomon species?
because of the species of this genus are very variable in shape, identification of them is confusing for me.
thank you for your help
samples area: Persian Gulf
thanks
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Dear Doctor Peyghan  The Isognomonidae are a group very variable with few species in your sea. I send you the page of this very interesting book and also the frontispiece.
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this cowrie was busily grazing on a reef flat located during the low tide. Would be happy if someone can identify the species. The spots are orangish and small on a white background. mantle has a dark brownish line on the periphery after which hairy structures are seen. 
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Hello Samuel - Your specimen is undoubtedly one Erosaria turdus and, more particularly, an Erosaria turdus winckworthi Sch. & Sch., 1938, corresponding to the most eastern population of this species (Oman-Pakistan-India). 
All the best, Gianluigi
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Hello
I have some species of the genus Isognomon fom Persian Gulf. 15 m depth.  from a shipwreck
I'm in doubt about them
Could you help me to identify it?
thank you very much
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Hi Soroor,
this is close to Isognomon legumen (Gmelin, 1791) 
Best regards
Deepak