Molecular Imprinting - Science method

In many researches, the mixture of MeOH and acetic acid (= 9 : 1 , for example) is commonly used, in order to remove the template molecular in molecular imprinted polymer (MIPs).

I wonder why this mixture solution is widely used, and what the meaning of this operation is.

I can imagine that the aim is to change the pH of slurry solution of MIP so that the structure of template molecular changes, and affinity and interaction between template molecular and monomer decreases.

But, I have a concern about my hypothesis.

In my research, I use 17β-estradiol (contains -OH) as a template molecular, MAA (pKa: about 4.6) as monomer. The interaction is related by hydrogen bond between "-OH of 17β-estradiol" and "-COOH of MAA".

If I take the pH condition of my reaction solution into consideration, I hypothesize that the lower pH leads to the more ratio of molecular form of MAA, so that the hydrogen bond get stronger.

However, the mixture of MeOH and acetic acid (the lower pH than the solvent of reaction solution, toluene) is used for removing molecular, according to the research which I refer to.

Are my hypotheses correct or incorrect?

the addition of the template in the preparation of the MIP led to a low cross-linking degree and low phase transition temperature

could someone elaborate on this?

I'm looking for a commercially accessible template that's suitable for OTA imprinting polymer and is also affordable.

molecular imprinted polymerization what will be the ratio of mixing the cross linker monomer and template to make good MIP

Hello, I'm biologist and currently working on MIP. I wanted to know is there any literature that mention the influence of monomer structure (for example size of molecule) on MIP selectivity and performance? I've seeh a lot of literature about influence of functional group of monomer but not about influence of its overall structure.

I'll gladly receive any recommendations on this question.

in other word what is the best cross linker used to activate the nano-particles for their further reaction with Molecular imprinted polymer

I'm working on magnetic molecular imprinting of proteins with chitosan and sodium alginate. At the first step, I coat the fe3o4 nanoparticles with sodium alginate and then coat the result with chitosan and protein. Then, I remove the protein to make MIPs. The problem is that the MIPs absorb both specific imprinted protein (albumin) and nonspecific protein (lysozyme). Does anyone know the reason or a certain method for this kind of imprinting?

AlBN - azobisisobutyronitrile

I am looking to buy Cortisol MIPs to use them in my research, I have seen a website can provide it but the price was too high ( 70k £). If you know any companies can provide this MIPs service please let me know.

Thank you

In the molecular imprinted method, if anybody works with unconductive polymer for electropolymerization and determination of electroactive analyze in the presence of K3Fe(CN)6/K4Fe(CN)6 should the increased current be followed or decreased current? please help me!

I used a liquid pesticide to make molecular imprinted polymer but did not form polymers, even using different solvents did not help.

I am looking at performing electropolymerization of molecular imprinted polymers using a template and a pyrrole. I need to add the template along with the dopant to my buffer solution before running glavanommetry. Must the template be soluble in the buffer, or could the template be just dispersed into it ?

Thank you,

Abbas

IN Molecular Imprinted polymer How can I determine the optimum ratio between the tempelate and the monomer also the amounts added. Is it just trial and error or do they have base which I can determine upon ? also the base upon which I choose the optimum solvent.

I made a molecular imprinted polymer ( MIP) and had characterized it using BET. the isoterm graph for adsorption-desorption are closed each other. The data of surface area from multi point BET plot is 261.611m2/g (slope 13,211, intercept 1,007e-01, r 0.999780, C 132.255). The average pore size 8.14759e+01A. Total pore volume 1.066e+00 cc/g for pores smaller than 1530.7 A (radius) at P/Po = 0.99371.

From BJH method adsorption the surface area =157.200m2/g, pore volume 1.008 cc/g, pore radius 15.278 A and from BJH method desorption the surface area 182.695 m2/g, pore volume 1.002 cc/g, pore radius 19.121 A.

which data should I use and why?

I don't know whether should use the BET or BJH and why. I read that BET to know the surface area and BJH to know the pore size and volume but still don't understand the reason.

Please help me.. thank you

MIP sensors usually suffer from lack of reproducibility, how we can improve it?

Hey, I want to ask what is the practical challenges for molecular imprinted polymer (MIP) sensors? Does it have good selectivity? How about consistency? Does it have major challenges during the manufacturing process? Any insights is welcome. Thanks.

Molecular biology, biochemistry, physical chemistry, molecular imprinting, Polymerization, molecular imprinting polymers 

Hello, As far I know that in molecular imprinting Theory People Usually Take a target molecule which will be imprint and then in the last step by using significant procedure they remove the target molecules. So whenever the imprinted thing will have the target molecule it will grab it. My question is is it possible to use solvents like IPA and Acetone to use as target molecule to imprint.

If I would like to introduce a specific molecular imprinted polymers with a transducer to develop a biosensor, then which type of transducers can I apply. What are the different physico-chemical changes produces after binding of a MIPs with its target analyte?

material i have-methaacrylic acid + methaacrylamide (1:3) ( monomers)

DMSO/methanol (solvent)

AMA ( initiator)

EDGA (cross-linker)

i have been following thermal method but polymerization is not happening. please guide. thank you 

I would like to make and take a rational decision while designing my first MIP (inshaa' Allah). I've learned during my MBA study that market research reports help a researcher to gain birds eye view for the market of interest. However, it's very costly for an early stage researcher like me to buy a market research report from a firm like bccmarket research. Accordingly, is there an open source panel that can provide me with a free of charge market research report?

I have synthesized an HPMIP with the help of MCM-48. But on mounting the cartridge (properly fitted with the fritts) on the SPE manifold, I still get a leakage with the eluate i.e, On conditioning the cartridge, I still get some of the white fluid with the eluate. Kindly suggest.

what is the exact concentration of materials/reactants needed to be added for the same.

Dear Researchers,

I hope this question finds you doing well.

I have coded an MIP model on Cplex, but when I run it, it gives a relaxed solution. However, the model has a global solution on another solver. I guess that I have to modify the run setup in Cplex to force Cplex to search for the global solution.

Best Regards,

Mahmoud

I am trying to figure out what typical ratios are for monomer/cross-linker/template solution in MIPs to have a starting point for my experiments. Also, if it appears there needs to be some modifications to the polymer, which of the three factors should be adjusted?

I wanted to use silica as support material for molecular imprinting. I wanted to skip the method of silynation and activation of silica. I prefered the readily available functionalized one.  I search for different functionalized silica.  I found the 3-(ethylenediamino) propyl functionalized silica gel.  I am not sure if it is possible to do molecular imprinting on its surface.  I hope I can have answers. I also hope to have more suggestions of what to use instead of doing the lengthy functionalization. Thanks a lot.

Template characteristics affects molecularly imprinted polymer characteristics if careful selection of reaction components is not done.

I have prepared two batches of blank MIP nanoparticles using an article as a reference, in the Batch-1, i have added MAA(0.475 mmol, 40 ul), EGDMA(1.11mmol, 209 ul) and AIBN(0.09 mmol i.e.15mg) into the 25 ml of ACN taken in 50 ml of RB flask step by step and then purged with nitrogen for 15 min similarly in Batch-2, keeping all same just by changing solvent i.e., 25 ml of ACN and NMP(N-Methyl-2-pyrrolidone) in 2:1 ratio mixture was kept for the reaction at 60 *c on the preheated oil bath for 24 hrs. After 24 hrs, the polymerization mixture is looking clear as it is in the initial. Now how can I determine whether polymerization has occurred or not?

I am making a MIP based on MAA as monomer with theophylline as template/ imprinted molecule. I used chloroform as the solvent, EDMA as the cross-linking agent, and the polymerization was initiated by BPO (benzoyl peroxide) (free radical polymerization). First i mixed all of the precursors and then it was polymerized at 60 C. I waited for over 5hours, but it didn't polymerized. it was suppose to be rigid, since i used a bulk polymerization method, but it wasn't. Most of the liquid was gone, leaving some small solids on the bottom of the vial. But they were so small, i couldn't take them out of the vial.

why the polymerization didn't occur?

If I would like to introduce phosphate specific molecular imprinted polymers with a transducer to develop a phosphate biosensor, then which type of transducers can I apply. What are the different physico-chemical changes produces after binding of a MIPs with its target analyte?

I need a computer program to form molecularly imprinted polymers and their design by using template molecules. Do you have any suggestions?

molecular imprinted polymer