Molecular Identification - Science topic

Phytochemical profile and antifungal activity of stems and leaves methanol extract from the Juncus maritimus Linn. Juncaceae family against some dermatophytes fungi AIP Conf. Proc. Abstract. Plants are one of the main sources of effective compounds used in medicinal treatments against fungi; among these plants are henna, thyme, and garlic. The main...

Background Kluyvera are gastrointestinal commensals and emerging opportunistic pathogens. Kluyvera are considered intrinsically resistant to ampicillin and early generation cephalosporins due to chromosomal β-lactamase encoding blaKLU genes, which are the proposed ancestors of modern CTX-M enzymes. Case Summary Here, we present a case of bacteremi...

The present study deals with the synthesis of silver nanoparticles from leaves Carica Papaya L. and its antibacterial activity against Staphylococcus sp. isolated from acne skin lesions. Staphylococcus sp. from the nodulocystic skin lesions were provided in youngsters among college students, in the age range of 18-24 years. Single colonies were fur...

Aims: The objective of this study was to identify bacterial strains isolated from the soil around the pine tree located in eastern Algeria in order to use them as lingo-cellulosic biomass degrading agents on one hand and biocontrol agents to preserve crops and improve yields on the other hand. Methodology and results: Over 14 bacterial isolates wer...

Mushrooms are critical fungi esteemed for their nutritional and medicinal properties and their wide array of genera and species. However, the phenotypic and molecular traits of mushrooms can intersect, making it vital to distinguish between beneficial and detrimental types. This study honed in on the phenotypic and molecular identification of selec...

Wastewater treatment plants (WWTPs) play a critical role in mitigating microbial contamination in aquatic environments. However, the persistence of pathogenic bacteria, including Vibrio spp., in treated effluents poses a significant public health risk. This study investigates the prevalence, seasonal variation, antibiotic resistance, and genetic di...

Toxoplasma gondiiis a globally significant parasite that affects various vertebrates, including livestock, posing health risks and economic losses, particularly in poorly managed regions. A total of 450 blood specimens were randomly collected from goats (Capra hircus) of Malakand Division, Khyber Pakhtunkhwa, Pakistan. Genomic DNA was extracted, an...

Aims: This study aimed to explore the antibiotic potential of Actinobacteria strain 4VH4, isolated from lichens in Vietnam, through comprehensive morphological, biochemical, and antimicrobial characterization. Methodology and results: Morphological analysis revealed diverse growth patterns across various media, with substrate mycelium colours rangi...

Mukaria sakaeratensis Piraonapicha & Chomphuphuang, sp. nov. is described based on male and female specimens recently collected in Nakhon Ratchasima, Thailand. The new species is herein described by an integrative approach combining morphological and molecular evidence. Genetic distance analyses revealed a potential barcoding gap (K2P) of 0.20-12.0...

Spoilage of apples continues to be a significant issue in the fruit industry. This study aimed to isolate and identify fungal species on deteriorated apples collected from three different locations in Ota market, Ota, Ogun State, Nigeria. A total of eighteen (18) samples of red delicious and Granny Smith apples with obvious spoilage were collected,...

The emergence and dissemination of antibiotic-resistant Klebsiella pneumoniae, particularly those are extended-spectrum beta-lactamase (ESBL) producers, thought to pose a serious threat to global health. This study aimed to isolate and identify the ESBL-producing K. pneumoniae from captive wild and migratory birds in Bangladesh along with their ant...

The Staphylococcus aureus complex is a group of bacterial species that share several biological and pathogenic characteristics. It mainly includes Staphylococcus aureus , as well as closely related species such as Staphylococcus argenteus and Staphylococcus schweitzeri. S. schweitzeri is the only species in this complex that predominantly occurs in...

Background Traditional ecological knowledge (TEK) held by Indigenous communities is increasingly recognised as a cost effective, locally adapted complement to instrument-based wildlife monitoring. In southern Gabon, hunter trackers routinely distinguish chimpanzee Pan troglodytes troglodytes and western lowland gorilla Gorilla gorilla gorilla faece...

Mycotoxins contribute to a substantial loss of global maize grain yields in terms of tonnes. However, in sub-Saharan Africa, screening of mycotoxin-producing fungi predominantly relies on culture-based methods, which are both time-consuming and labour-intensive. This study examined the major fungal species responsible for aflatoxin production in ma...

Small carnivorous fishes serve as important mesopredators in coral reef ecosystems. However, the habitat and prey availability degradation within these ecosystems has intensified the trend of body size miniaturization and interspecific competition among these species. To better understand the food selection and resource partitioning strategies of m...

Sarazaremanesh | Javad Ahmadi-Shokouh | Hamideh Dashti|mortezachobuin A compact hexagonal antenna for wireless communication is proposed in this paper. The antenna resonates at 2.45 GHz (ISM) band. The antenna has an overall dimension of only 7.6cm X 5.8cm which shows a good size reduction compared to antennas operating in the WLAN band. The simple...

Sarazaremanesh | Javad Ahmadi-Shokouh | Hamideh Dashti|mortezachobuin A compact hexagonal antenna for wireless communication is proposed in this paper. The antenna resonates at 2.45 GHz (ISM) band. The antenna has an overall dimension of only 7.6cm X 5.8cm which shows a good size reduction compared to antennas operating in the WLAN band. The simple...

The sugarcane woolly aphid, Ceratovacuna lanigera Zehntner, 1897 (Hemiptera: Aphididae: Hormaphidinae) is a major agricultural pest originally described from Java, Indonesia. We report the first confirmed occurrence of C. lanigera in South Korea, supported by morphological characterization using light microscopy and molecular identification based o...

Dark-brown irregular necrotic spots, 2–5 cm in diameter, were observed on the Sultana and Narc Black varieties of grape (Vitis vinifera) in Pishin region (30°35’36.0"N 67°00’25.0"E), Pakistan. Infected leaf samples were collected, disinfected with 2% sodium hypochlorite, and plated on potato dextrose agar plate. The colony growth initially appeared...

Background: Blastocystis is a common protozoan often found in the gastrointestinal tract of humans. The presence of Blastocystis in the female genital tract—has been reported three times, but the genetic material of the protozoan from this site has been identified only once. Methods: Patients with cervical erosions were enrolled in the study. Sampl...

Background: Antioxidants can be obtained from various sources, including bacterial pigments, which are natural compounds with great potential in various applications. Therefore, this study aimed to explore microbial pigments as antioxidants from various sources, to identify isolates macroscopically, microscopically, and molecularly, as well as to c...

Tobacco ( Nicotiana tabacum L.) is an economically important crop, widely cultivated in China. Since 2021, a new leaf spot disease has been seen frequently in Enshi, Xiangyang, Yichang, and Shiyan, all in Hubei Province, China. Diseased leaves exhibiting roundish lesions with greenish-yellow edges were collected to determine the causal agent in sum...

Volvariella volvacea, generally known as paddy straw mushroom, is a nutritious and edible mushroom that is commercially cultivated in tropical countries such as the Philippines. This study highlights the molecular identification and the optimal nutritional and physical culture conditions of the three isolates of V. volvacea from the Philippines. Ge...

Canine bufavirus (CBuV) was initially detected in dogs with respiratory symptoms and later identified in fecal samples, suggesting that it could be an enteric pathogen. Several studies have shown that CBuV can be present in both healthy and diarrheic dogs, raising questions about whether CBuV is a primary enteric pathogen. In this study, we identif...

Due to their biotechnological importance in different industrial fields, xylanases from microorganisms have been the focus of great attention. In this regard, bacterial strain, SHB_02, one of the best xylanase producer isolated during a screening study from soil, was identified as Streptomyces tendae (GenBank accession no. OP893637) based on phenot...

The ascarid nematode, Toxocara canis, which causes Toxocariasis in dogs, is a member of the Toxocaridae family and genus Toxocara, and that is accidentally infects humans. The study was conducted to investigate the presence of T. canis in dogs in Karbala, Iraq. The study extended from January to October, 2023. This project is the first of its kind...

Research into ticks that feed on small mammals is important because they can be vectors of pathogenic bioagents that infect animals and humans. In this study, small mammals were captured in the Maracanã Environmental Protection Area, in the eastern Amazon biome (São Luís, Maranhão), and were visually inspected to detect the presence of ectoparasite...

An assessment of the fish species composition was conducted during the austral summer of 2019 in the Southwest Atlantic sector of the Southern Ocean (FAO Fishing Areas 48.1–48.4). Samples were collected from stations systematically spaced across the Scotia Sea region using a fine meshed pelagic trawl (“Macroplankton trawl”) towed through the ~200-0...

Prostate cancer (PCa) is a major, and increasingly global, health concern with current screening and diagnostic tools’ severe limitations causing unnecessary, invasive biopsy procedures. While gas chromatography–mass spectrometry (GC-MS) has been used to detect urinary volatile organic compounds (VOCs) associated with PCa, efforts to identify consi...

Honey bees, vital agricultural pollinators, suffer significant losses due to the pathogen Nosema ceranae. Fumagillin, utilized to prevent nosemosis, poses a hazardous risk. This study aims to investigate the potential of natural thymol and propolis extract in combating Nosema infection in honey bee colonies. The evaluation will be conducted thr...

Genome skimming is a promising sequencing strategy for DNA-based taxonomic identification. However, the lack of standardized datasets for benchmarking genome skimming tools presents a challenge in comparing new methods to existing ones. As part of the development of varKoder, a new tool for DNA-based identification, we curated four datasets designe...

Narrow-barred Spanish mackerel (Scomberomorus commerson) is one of the most important commercial fish species in the Persian Gulf and Oman Sea. Parasites of the genus Hysterothylacium (Ascaridoidea: Anisakidae) are common ascaridoid nematodes of fish-eating birds and sea mammals. The aim of this study is to identify morphological and molecula...

Background In October 2022, black pustules were observed on the lower surface of sponge gourd (Luffa cylindrica) leaves in Kafer El-Dawar, North Egypt. Symptoms included abundant black pustules containing fungal conidia on plant leaves, which eventually led to the infected leaves drying out and dying. Results Two causal fungi were isolated from sy...

This study reports the molecular identification of a tambacu—a hybrid of Colossoma macropomum and Piaractus mesopotamicus—in the floodplain of the upper Paraná River using molecular markers. The PCR‐GEL method, based on the analysis of COI and TROP fragments, successfully characterized the hybrid, while PCR‐SEQ, employing RAG2 and TROP markers, ide...

p> Background. Chili pepper ( Capsicum spp.) cultivation extends throughout Mexico, hosting a wide variety worldwide. However, crop health is reduced by the incidence of pathogenic microorganisms such as those of the Fusarium genus, which is one of the main agents causing vascular wilt and mortality of plant species. The use of molecular tools allo...

DNA barcoding is a powerful tool for accurately identifying marine turtle species, especially when morphological identification is challenging, owing to insufficient clues in the samples available. This study focused on two sea turtle samples of dead adults washed ashore and babies hatching out from nests, which pose a challenge for morphological i...

Background Largemouth bass (Micropterus salmoides, LMB) is an economic fish in China, which has developed into many different cultured populations in the past decades. These populations exhibit different growth rates, morphological traits, stress resistance, and genetic diversity. Analyzing genetic diversity and molecular identification of these po...

MRSA is a highly prevalent bacterial infection that continues to be a leading cause of morbidity and mortality worldwide. Nanoparticles (NPs) are one of the promising strategies to deal with bacterial infections. The synergism of nanoparticle oxides and other natural materials showed complementary effects and enhanced antibacterial activity. So, th...

Aerobic denitrifying bacteria can effectively cope with the challenge of dissolving nitrogen in wastewater. High-performance aerobic denitrifying bacteria were isolated using the plate streaking method and subsequently evaluated and identified based on nitrate removal efficiency, nitrite accumulation, growth characteristics, morphological analysis,...

Symbiont bacteria in mangrove litter include bacteria associated with mangrove plants. These bacteria help mangrove plants by increasing the nutrients available in the environment, reducing humidity, and converting insoluble phosphate into soluble forms. Other bacteria found in mangrove litter include nitrifying, decomposition, and organic compound...

This study aimed to establish a fungal symbiont culture collection for coastal dune conservation and determine the diversity and distribution of endophytic fungi in rocky coastal dunes at Gimnyeong and Woljeong in the Jeju Islands. Endophytic fungi can promote plant growth and induce systemic resistance to environmental stress and infections in hos...

Lignin is an abundant phenolic compound that has –OH groups and is found in many sources such a tree. Lignolytic enzyme plays an important role in dye bioremediation. Methylene blue is a dye generally applied in the industry to produce a blue color. However, it also causes pollution and waste. To prevent environmental pollution, it is necessary to...

Sponge-derived fungi are considered a promising source for lead compounds in drug discovery. This study aimed to identify fungi isolated from the sponge Phyllospongia foliascens collected in Amed, Bali, Indonesia, and investigate the antimicrobial activity and secondary metabolites of their methanolic extracts. The identification of pure fungal iso...

Mangrove diseases are the most neglected threats that might occur in Indonesia’s mangrove ecosystems. During a field study at Mangunharjo village, Semarang City, Central Java, Indonesia, some pests and diseases were found in Rhizophora mucronata, including leaf spot diseases commonly caused by fungi. This study aimed to isolate, determine, and iden...

Hymenopteran insects play a vital role in facilitating cross-pollination, which boosts genetic diversity and helps plants to reproduce. A total of 932 samples were collected from the study area, which belonged to 14 families and 52 species. Species abundance study revealed that the Apidae family showed the highest abundance (30.93), followed by Hal...

The increasing prevalence of multidrug-resistant (MDR) bacteria highlights the urgent need for new antimicrobial agents. In this study, Bacillus species were isolated from mangrove sediments along the Red Sea coast in Jeddah, Saudi Arabia. A total of 30 isolates were screened for antibacterial activity against four MDR pathogens: Staphylococcus aur...

Marine endophytic fungi are microorganisms that inhabit marine plant tissues without harming their hosts. These fungi can produce exopolysaccharides (EPS), which have potential applications in food, pharmaceuticals, and biomaterials. This study aims to screen and characterize EPS-producing marine endophytic fungi. Ten fungi isolates from seaweed, s...

Aims Drought stress significantly impacts crop yields and food security, particularly for legumes like lentil (Lens culinaris), which depend on symbiotic relationships for nitrogen fixation. This study investigated the synergistic impacts of silicon (Si) and plant growth-promoting non-rhizobial bacteria on enhancing drought resilience in the lentil...

In spatial’omics, highly confident molecular identifications are indispensable for the investigation of complex biology and for spatial biomarker discovery. However, current mass spectrometry imaging (MSI)-based spatial ‘omics must compromise between data acquisition speed and biochemical profiling depth. Here, we introduce fast, label-free quantum...

Introduction The yeast Candida vulturna is a member of the Candida haemulonii species complex, like its close relative Candida auris. Members of this species complex, including C. vulturna, often display reduced susceptibility to one or more antifungal classes. Human invasive infections by C. vulturna are increasingly reported in various countries,...

Antibiotics or antimicrobial agents are the most significant utilized secondary metabolites, which are commonly synthesized by soil bacteria and fungi and found to be effective. Most of the antibiotics used are derived from the soil bacteria and Actinomycetes. Antibiotic resistance poses a serious global health threat, making infections harder to t...

Rapid, effective, and specific identification of clinical and environmental bacterial pathogens is of major importance for their control. Traditionally, bacteria have been identified by phenotypic methods based on morphological, biochemical, and metabolic properties. While these methods are very useful in clinical practice, they have limitations in...

Akabane disease is an arthropod-borne disease caused by Akabane virus (AKAV), which is characterized by abortion, premature birth, stillbirth, congenital arthrosis, and hydrocephalic anencephalic syndrome in pregnant cattle and sheep. The occurrence of AKAV was proved by RT-PCR amplification based on AKAV S fragment, virus isolation, cells inoculat...

Background The coronavirus disease‐19 (COVID‐19) pandemic has affected global health, influencing the prevalence of different respiratory pathogens. The aim of this study is to evaluate the distribution of agents causing acute respiratory infections in children under 5 years old before and after the COVID‐19 pandemic in Praia, Cabo Verde, and to de...

The yak (Bos grunniens), prevalent at an altitude between 3000 and 5000 m above sea level, provides the local inhabitants with meat, milk, leather, fuel (dung), and transport. However, intestinal zoonotic parasites seriously endanger its holistic well-being. The prime concern of this study is to investigate the prevalence of four globally ubiquitou...

Enterococci are opportunistic zoonotic pathogens. Dairy cattle and farm environments are considered important sources of Enterococcus spp. Here, we detected biofilm-forming Enterococcus faecalis circulating in dairy cattle and farm environments, followed by the detection of their virulence genes, antibiogram phenotype analysis, and genotype charact...

Background: Eggs are an affordable dietary staple in Nigeria, yet they pose a potential public health risk when contaminated. This study assessed the prevalence of pathogenic bacteria and their antimicrobial resistance profiles on eggshells from markets in Lagos, Nigeria. Methods: A total of 51 table eggs were randomly sampled from poultry farms an...

Background Larval stages of carnivore cestodes in rodents have been extensively studied for decades, primarily based on morphological indicators. Numerous datasets, particularly from Central Europe, exist on this topic. Traditionally, the shape, number, and size of hooks have been key distinguishing features. However, recent findings suggest that m...

Introduction: Ceftaroline is a fifth‐generation cephalosporin that was recently introduced into the Egyptian market for the treatment of methicillin‐resistant Staphylococcus aureus (MRSA) infections. However, limited data are available regarding the susceptibility of MRSA isolates in Egypt to this antibacterial agent. This study aimed to determine...

Genus Anomala Samouelle, 1819, is a notorious phytophagous pest that harms plant leaves and roots. In South Korea, eight of 13 species have been designated as pest species and have been managed in agriculture, horticulture, and forestry fields. Accurate identification of pest species is crucial for establishing appropriate pest management strategie...

The soybean cyst nematode (Heterodera glycines) is the most economically damaging soybean pathogen in the United States and other significant soybean-producing countries. Its resistance, adaptation rate, and capacity for causing extensive yield reduction are the primary characteristics that enable it to be a hardy pest at times, necessitating the u...

As a medicinally significant species within the Cucurbitaceae family, Trichosanthes kirilowii is currently lacking comprehensive chloroplast genomic resources that are essential for conducting evolutionary and phylogenetic studies. In this study, we present a characterization of its complete chloroplast genome, which consists of a circular DNA mole...

Given the severe side effects of prolonged morphine use, the search for safer alternatives is a global priority. This study investigates marine bacteria from sediments along the Bejaia coast, Algeria, to identify opioid-like bioactive compounds with potential analgesic properties. A total of 45 bacterial strains were isolated using six different cu...

Post-harvest decay of Carica papaya L. is the primary cause of deterioration in papaya quality and the low economic impact of this sector in Cameroon. Field surveys conducted by teams from the Ministry of Agriculture and Rural Development (MINADER) in Cameroon have primarily associated these decays with fungal attacks. However, to date, no methodol...

Petroleum is a vital and strategic energy resource for boosting a country’s GDP. Despite its high economic value, it is considered a primary factor in environmental deterioration. Bioremediation strategies employ indigenous microbial strains to propose an economical and sustainable alternative to conventional remediation practices. The current stud...

Article History Predatory nematodes are free-living organisms with significant potential to functional as biological control agents against plant-parasitic nematodes. This study investigated the diversity, molecular characterization, and biocontrol potential of predatory nematode from natural soil ecosystems in Thailand. Comprehensive soil sampling...

Heavy metals (HMs) contamination is a major issue produced by industrial and mining processes, among other human activities. The capacity of fungi to eliminate HMs from the environment has drawn attention. However, the main process by which fungi protect the environment against the damaging effects of these HMs, such as cadmium (Cd), is still unkno...

Flies and maggots are of medical importance, and it is often necessary to identify them at species level. Conventionally, this is carried out based on morphological features using taxonomic keys. However, identification of maggots based on morphology is difficult and required entomological expertise is often lacking in clinical settings. Molecular...

The search for eco-friendly alternatives to conventional petroleum-based materials has intensified in an era marked by a growing global awareness of environmental sustainability. This study addresses the critical need for molecular identification and characterization of fungi sourced from a tempeh commercial starter culture for their potential role...

The study aimed to compare and analyze the ribulose-1, 5-bisphosphate carboxylase (rbcL) sequences of Spatholobus suberectus Dunn and its adulterants and to explore whether rbcL could be used as the DNA barcode to effectively identify them. The obtained sample rbcL sequences were compared with those in the NCBI database, and the alignment results w...

The present study, entitled “Morphological, Biochemical, and Molecular Identification of Beneficial Soil Bacteria in Sorghum Rhizosphere” aimed to characterize rhizospheric bacteria associated with sorghum, and assess their potential role in plant health. Soil samples were collected from three distinct locations in Bhubaneswar, Odisha, Smrutivan Pa...

Contamination of agricultural products by Fusarium species is a significant concern and is commonly found in various agricultural products. They cause severe economic losses in the products, and contaminate and threaten human and animal health due to the toxins they produce. Therefore, determining species diversity in various agricultural products...

Mammalian hair is a source of biological information and can be used in genetic, toxicological, hormonal, and ecological studies. However, non-invasive collection methods are still little explored. This study aimed to describe and validate a passive methodology for collecting hair from jaguars (Panthera onca) and evaluate its viability for differen...

Nerium oleander L is a long-lasting flowering vegetatively propagated ornamental plant of the Mediterranean region, where it is a major imported flowering pot plant. Only a few bacteria can infect it such as Pseudomonas savastanoi pv. nerii, Agrobacterium tumefaciens and Xylella fastidiosa. Between 2018 and 2022 we collected several infected plant...

Objective: To develop scanning electron microscopy-based Trainable Weka (Waikato Environment for Knowledge Analysis) Intelligent Segmentation Technology (SEMTWIST), an open-source software tool, for structural detection and rigorous quantification of wound biofilm aggregates in complex human wound tissue matrix. Approach: SEMTWIST model was standar...

Introduction Salinity is a major issue affecting agricultural lands, leading to reduced crop productivity and soil degradation. One approach to mitigate the effects of salinity is utilizing PGPR. This study aimed to isolate and identify indigenous PGPR from rhizosphere soil and evaluate their effects on the growth indices of chamomile under saline...

Plant chitinase, a member of the pathogenesis-related proteins and glycoside hydrolase family 19, plays a crucial role in plant resistance to pathogenic fungi. In this study, we isolated a chitinase gene encoding PaChi from Picea asperata, containing an open reading frame of 768 bp that encodes 255 amino acid residues. PaChi includes the chitin-bin...

Pasteurella species are well-known opportunistic bacteria that inhabit the microbiota of the oral cavity and upper respiratory tract of cats and have been related to a set of pet-associated diseases in addition to humans. Most studies involving feline pasteurellosis have been described as case reports and species identification based on classic phe...

Background: Brucellosis, caused by Brucella species, remains a significant zoonotic and economic challenge in Iran, where Brucella melitensis is increasingly detected in dairy cattle due to close contact with infected sheep and goats. The persistence of B. melitensis highlights the limitations of existing control strategies and the need for improve...

Maize (Zea mays L.) is a vital cereal crop globally and in India, where its productivity is often limited by biotic stresses such as banded leaf and sheath blight (BLSB), caused by Rhizoctonia solani f. sp. sasakii. In this study, BLSB-infected maize samples were collected from Kalyani, West Bengal, and the pathogen was isolated, morphologically an...

Arbuscular mycorrhizal fungi (AMF) are obligate biotrophs that form a symbiotic and mutualistic relationship with most terrestrial plants, playing an important role in plant growth, nutrient acquisition, and ecosystem stability. This review synthesizes current knowledge on AMF colonization in plants within New Zealand ecosystems, including the chal...