Molecular Biology - Science topic

We are excited to invite you to submit your research collections to our special issue on "Genomics and Molecular Biology of Insect-Plant Interactions https://www.techscience.com/phyton/special_detail/genomics with Phyton-International Journal of Experimental Botany. Submissions are now open, and we look forward to receiving your contributions. Plea...

Low and persistent levels of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA/protein/virus can be detected in clinical samples months after infection, possibly related to the emergence of SARS-CoV-2 variants or development of long coronavirus disease. Here, we present a protocol to detect low levels of viral RNA together with prote...

Average treatment effect has a very important role to measure the differences in mean outcomes between units assigned to the treatment and units assigned to the control and it has applications in different scientific domains such as molecular biology, econometrics, machine learning. Accordingly, from previous studies in biological networks, it has...

Stem cells regenerate differentiated cells to maintain and repair tissues and organs. They also replenish themselves, i.e. self-renewal, for the regenerative process to last a lifetime. How stem cells renew is of critical biological and medical significance. Here we use the skeletal muscle stem cell (MuSC) to study this process. Using a combination...

El Congreso Internacional en Ciencias Agrícolas, tiene por objetivo abordar aspectos de los avances y desarrollo tecnológico en las ciencias agrícolas, promover la difusión del conocimiento de la investigación científica, técnica y experiencias de productores en diversos temas de interés de producción agrícola y disciplinas relacionadas. Este event...

The European Molecular Biology Laboratory's European Bioinformatics Institute (EMBL-EBI) is one of the world's leading sources of public biomolecular data. Based at the Wellcome Genome Campus in Hinxton, UK, EMBL-EBI is one of six sites of the European Molecular Biology Laboratory (EMBL), Europe's only intergovernmental life sciences organisation....

This poster was presented at the 50th Philippine Society for Biochemistry and Molecular Biology (PSBMB) Annual Conference. We reviewed metabolomics studies from published works in the Philippines and abroad, and employed exploratory analysis to determine research trends, themes and analytical platforms.

This is a detailed experimental protocol for the transformation of plasmid DNA into competent E. coli cells, specifically using the DH5α strain. The protocol encompasses the entire process, from the preparation of LB agar plates supplemented with ampicillin to the final inoculation of transformed cells in LB broth. Key steps include the careful tha...

Lung carcinoma is one of the leading causes of cancer deaths worldwide for both men and women. Established risk factors & probable risk factors, which are implicated in the occurrence of lung carcinomas have been identified and evidently present in the populace, especially in our Indian scenario, e.g., tobacco chewing, smoking & many more. With the...

Clustering genes in single-cell RNA sequencing plays a pivotal role in unraveling a plethora of biological processes, from cell differentiation to disease progression and metabolic pathways. Traditional time-domain methods are instrumental in certain analyses, yet they may overlook intricate relationships. For instance, genes that appear distinct i...

Individuals with PhDs and postdoctoral experience in the life sciences can pursue a variety of career paths. Many PhD students and postdocs aspire to a permanent research position at a university or research institute, but competition for such positions has increased. Here, we report a time-resolved analysis of the career paths of 2284 researchers...

Avian infectious bronchitis is an acute respiratory disease of poultry of particular concern for global food security. Investigation of Infectious Bronchitis Virus (IBV), the causative agent of avian infectious bronchitis, via reverse genetics enables deeper understanding of virus biology and a rapid response to emerging variants. Classic methods o...

High-resolution protein structure determination by experimental techniques is notoriously costly and labor intensive. This problem is mostly solved with arrival of deep-learning-based computational prediction by AlphaFold2 but only for those proteins with enough naturally occurring homologous sequences. Here, we attempt to close the remaining gap b...

Введение. Stenotrophomonas maltophilia является условно-патогенным микроорганизмом, обладающим природной устойчивостью к широкому спектру антибиотиков. Бактерия ассоциирована с рядом серьёзных заболеваний и вносит значимый вклад в патогенез полимикробных инфекций. S. maltophilia обладает широким набором факторов вирулентности, информация о которых...

The azalea lace bug Stephanitis (Stephanitis) pyrioides (Scott, 1874) (Hemiptera: Heteroptera: Tingidae) originates from the East Palaearctic. In 2020, this alien species was recorded for the first time in the European part of Russia (Krasnodar Territory, Sochi), notably attacking Rhododendron × hybridum hort. It most likely arrived there with plan...

The presence of Acinetobacter baumannii as an opportunistic pathogen has become a major concern in healthcare settings. This bacterium is frequently responsible for a range of infections, including pneumonia, meningitis, bloodstream infections, urinary tract infections, and wound infections.. . etc, in individuals with weakened immune systems. Its...

The sugar kelp Saccharina latissima has received intense scientific attention over the last decades. In recent years, interest in cultivation of the species has strongly increased in the North Atlantic Ocean and the Eastern Pacific Ocean, driven by the great potential of S. latissima to be utilised for various industrial applications, including foo...

The application now is open for MGBS speakers in 2024 We are pleased to announce that we are now accepting new applications for MGBS speakers 2024. The seminars will be held in March and April (weekly seminars). Interested speakers who would like to present their research are encouraged to apply online at https://mgbsonline.wixsite.com/mgbs2023/ap...

T-prolymphocytic leukemia (T-PLL) is a rare malignancy of mature T-cells with distinct clinical, cytomorphological, and molecular genetic features. The disease typically presents at an advanced stage, with marked leukocytosis, B symptoms, hepatosplenomegaly, and bone marrow failure. It usually follows an aggressive course from presentation, and the...

Introdução/Objetivo: O diagnostico precoce das meningites agudas impacta na conduta médica terapêutica, e a identificação da etiologia fornece subsídios para adequação da terapia antimicrobiana. Objetivamos avaliar o impacto dos métodos moleculares e da cultura na identificação etiológica e na modificação da terapia antimicrobiana e antiviral inici...

To study the chronic physiological adaptation of weight training program on performance variable of adolescence boys was analyzed in this study on influence weight training on performance of penalty kick of adolescence boys in football. The study was carried on 40 boys from thane district, Maharashtra. The average age of the boys was 14.92 years....

RegulonDB is a database that contains the most comprehensive corpus of knowledge of the regulation of transcription initiation of Escherichia coli K-12, including data from both classical molecular biology and high-throughput methodologies. Here, we describe biological advances since our last NAR paper of 2019. We explain the changes to satisfy FAI...

Breast cancer, a leading cause of cancer-related deaths worldwide, has been extensively studied through the lens of molecular biology. Researchers have made significant strides in uncovering the intricate molecular mechanisms driving breast cancer initiation, progression, and treatment response. Molecular profiling has identified various subtypes o...

Previous studies of the murine Ly49 and human KIR gene clusters implicated competing sense and antisense promoters in the control of variegated gene expression. In the current study, an examination of transcription factor genes defines an abundance of convergent and divergent sense/antisense promoter pairs, suggesting that competing promoters may c...

The presence of a mutation in the BRCA1/2 genes makes it possible to use platinum drugs and PARP inhibitors in patients with pancreatic cancer. Conducting molecular genetic testing allows you to assess the risk of developing malignant neoplasms in a patient and his relatives. We present a series of clinical cases of patients with pancreatic cancer...

Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures—yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identif...

Plant architecture is an important feature for agronomic performance in crops. In maize, which is a monoecious plant, separation of floral organs to produce specific gametes has been studied from different perspectives including genetic, biochemical and physiological. Maize mutants affected in floral organ development have been key to identifying g...

This essay introduces the concept of "Dynamic Genostability," a hypothesis that redefines life on Earth as the manifestation of DNA's quest for perpetuation. Contrary to the traditional organism-centric view of evolution, we propose that organisms are merely transient vehicles for DNA, which seeks to maintain its low-entropy state amidst environmen...

Introduction Alzheimer’s disease (AD) is a complex neurodegenerative disease with high heritability. Compared to autosomes, a higher proportion of disorder-associated genes on X chromosome are expressed in the brain. However, only a few studies focused on the identification of the susceptibility loci for AD on X chromosome. Methods Using the data...

Ion channels are key signaling proteins found throughout the body; they are critical in many, wide-ranging physiological processes, from gene expression, sensory perception and processing to the cardiac action potential. When ion channel activity goes awry, for example, via mutation, damage or disrupted homeostasis, the outcome can result in causat...

The object of the study is the Eastern part of the Western Fore-Balkan (Northwestern Bulgaria). In previous gas chromatography (GC) studies abnormalities in the content of C2-C5 alkanes in the surface layers of soils were found. In the present study, GC methods were combined with microbiological analyses of ten sample points. Both, GC and microbiol...

Respiratory organs are important to investigate in Solen sp. due to the fact that literacy is hard to find beside unclear data information. Excellent knowledge of the respiratory organs in biota has become one of the impact factors for knowing its potential in adaptability and ecology. This study aimed to describe the anatomical respiratory organ b...

The European Nucleotide Archive (ENA; https:////www.ebi.ac.uk//ena) is maintained by the European Molecular Biology Laboratory's European Bioinformatics Institute (EMBL-EBI). The ENA is one of the three members of the International Nucleotide Sequence Database Collaboration (INSDC). It serves the bioinformatics community worldwide via the submissio...

Background Inflammation plays an important role in the pathogenesis of atrial fibrillation (AF) after myocardial infarction (MI). The role of USP38, a member of the ubiquitin-specific protease family, on MI-induced atrial inflammation, fibrosis, and associated AF is unclear. Methods In this study, we surgically constructed a mouse MI model using U...

Flow cytometry is a single-cell based technology aimed to quantify the scattering of light and the emission of multiple fluorescence signals by individual cells, biological vesicles, or synthetic microscopical particles when examined one by one at high speed using lasers or other suitable illumination sources [...]

Recent discoveries in the field of molecular biology are focused on phenomena like chromatin condensation, histone (H) modification, and deoxyribonucleic acid (DNA) methylation, as well as the action of small non-coding ribonucleic acid (RNA), which together belong to the branch of epigenetics. The term “epigenetics” was coined in 1940 by Conrad Wa...

The concept of “organism” has been central to modern biology, with its definition and philosophical implications evolving since the nineteenth century. In contemporary biology, the divide between developmental and physiological approaches and evolutionary approaches has influenced the definition of organism. The convergence between molecular biolog...

En los últimos años, el estudio de la microbiología aplicada ha incorporado como un aspecto fundamental la utilización de técnicas moleculares que apoyan los procesos de identificación, caracterización, bioprospección y manejo o control de microrganismos. En este manual se presentan 49 experimentos distribuidos en 10 laboratorios que apoyan estos p...

The article describes the prevalence of inherited neuromuscular disease, specifically neural amyotrophy, or Charcot-Marie-Tooth disease, its key clinical and neurological features, promising blood biomarkers for future diagnosis and therapeutic strategy assessment, along with current treatment methods under investigation, taking into account gene t...

Over the past several decades, there have been a number of national and international meetings on waterborne diseases. Conclusions from these meetings often seem remarkably similar and suggest little progress in the field of water and health. This is both a true and a false premise, as our ability to use molecular tools to describe microbial commun...

Congenital toxoplasmosis is a consequence of acute toxoplasmosis in a pregnant woman. When assessing the risk of сongenital toxoplasmosis during pregnancy, methods for determining specific antibodies, the genetic material of the pathogen (polymerase chain reaction method), immunoglobulins G avidity are used. Molecular genetic methods have the great...

Background Alström syndrome (AS) represents an exceptionally rare genetic disorder characterized by a constellation of features including cardiomyopathy, progressive hearing and vision impairment, as well as obesity. This study seeks to elucidate the genetic underpinnings of this syndrome within the Saudi Arabian population. Methods Employing an e...

Monitoring the circulation of the measles virus and studying its genetic diversity is an important component of the measles elimination program. A methodological approach to molecular genetic studies and their interpretation in the measles surveillance was developed in the early 2000s. During its development, clear areas of circulation of each geno...

The goal of the methylation classifier in brain tumor classification is to accurately classify tumors based on their methylation profiles. Accurate brain tumor diagnosis is the first step for healthcare professionals to predict tumor prognosis and establish personalized treatment plans for patients. The methylation classifier can be used to perform...

Recent advances in Large Language Models (LLMs) have presented new opportunities for integrating Artificial General Intelligence (AGI) into biological research and education. This study evaluated the capabilities of leading LLMs, including GPT-4, GPT-3.5, PaLM2, Claude2, and SenseNova, in answering conceptual biology questions. The models were test...

Data-driven identification of functional relationships between cellular properties is an exciting promise of single-cell genomics, especially given the increasing prevalence of assays for multiomic and spatial transcriptomic analysis. Major challenges include dealing with technical factors that might introduce or obscure dependencies between measur...

Asterias amurensis has attracted widespread concern because of its population outbreaks, which has impacted fisheries and aquaculture, as well as disrupting local ecosystems. A high-quality reference genome is necessary to better investigate mechanisms of outbreak and adaptive changes. Combining PacBio HiFi and Hi-C sequencing data, we generated a...

Stem cells regenerate differentiated cells to maintain and repair tissues and organs. They also replenish themselves, i.e. self-renewal, for the regenerative process to last a lifetime. How stem cells renew is of critical biological and medical significance. Here we use the skeletal muscle stem cell (MuSC) to study this process. Using a combination...

This document is the result of the deliberations of the Committee on Emerging Pathogens and COVID-19 of the Illustrious Official College of Physicians of Madrid (ICOMEM) regarding the current situation of tuberculosis, particularly in Spain. We have reviewed aspects such as the evolution of its incidence, the populations currently most exposed and...

Introduction: Mathematical model can be used to model complex biological processes, and have shown potential in describing apoptosis in chondrocytes. Method: In order to investigate the regulatory mechanisms of TNF signaling pathway in regulating chondrocyte apoptosis, a fractional-order differential equation model is proposed to describe the dynam...

Phage display is a molecular biology technique that allows the presentation of foreign peptides on the surface of bacteriophages. It is widely utilized for applications such as the discovery of biomarkers, the development of therapeutic antibodies, and the investigation of protein–protein interactions. When employing phages in diagnostic and therap...

Background: The breast cancer (BC), in spite of tremendous advances has continue to remain an enigma. The major change in understanding of BC has taken place after understanding of molecular biology. Aims and Objectives: The objectives are as follows:(1) To categorize the incidence of triple negative BC (TNBC) in hospital based data. (2) To study t...

Metastasis and doxorubicin resistance are challenges in the clinical diagnosis and treatment of osteosarcoma, the mechanisms underlying these phenomena remain unclear. In this study, we found that DLX2 is highly expressed in metastatic osteosarcoma and is closely related to clinical prognosis. Knockdown of DLX2 inhibited tumor proliferation and mig...

The microbiological diagnosis of pleural effusion is based largely on classical microbiology methods, but these methods have a high rate of false negative results. Some previous studies have shown improved diagnostic performance for pathogens such as Streptococcus pneumoniae using molecular biology methods. We present the use of a multiplex PCR pla...

Transcription factors (TFs) play a pivotal role in gene expression, and their DNA binding is the prerequisite to instigating gene transcription. Here, we present a protocol that exploits the proximity ligation assay technique to measure the DNA-binding activities of TFs in situ at the single-cell resolution. We describe steps for immunostaining wit...

Faced with an enlarged lymph node, many pathologists are puzzled by the question of whether it is benign or malignant. Our practical experience has shown that alone histological knowledge of the lymph node is not sufficient to diagnose their pathology. Recognizing the extremely subtle histological changes of reactive lymph nodes is decisive in the...

Aim : to identify specific associations between genes polymorphism associated with sex hormone-binding globulin (SHBG) level and breast cancer (BC) of various molecular biological subtypes. Materials and Methods . The retrospective comparative study was conducted using specimens collected from 261 patients with BC of two molecular biological subtyp...

As central nervous system (CNS)-related disorders present an increasing cause of global morbidity, mortality, and high pressure on our healthcare system, there is an urgent need for new insights and treatment options. The endocannabinoid system (ECS) is a critical network of endogenous compounds, receptors, and enzymes that contribute to CNS develo...

We present a protocol to induce Cre-dependent transgene expression in specific cell types in the rat brain, suppressing a leak expression in off-target cells, by using a flip-excision switch system with a unilateral spacer sequence. We describe steps for construction of transfer plasmids, preparation of adeno-associated viral vectors, intracranial...

By enabling rapid label-free detection of biochemical substances, surface plasmon resonance (SPR) sensors have the potential to become an important tool in molecular biology and medical diagnostics. In this work, a high performance SPR fiber sensor based on metallic elliptical nanohole and circular nanodisk binary array has been proposed and studie...

The IAC Quarantine Complex is responsible for carrying out the quarantine of plant materials that will be introduced in Brazil by private companies or public institutions, with the objective of scientific research. The materials are analyzed for pathogenic agents, as well as sown in a greenhouse for growth analysis and to verify possible pathogen s...

Critical evaluation of how and when to include anthracyclines in preoperative chemotherapy is becoming more relevant in an era when the molecular genetic approach not only allows for the development of biologically targeted therapeutics, but also implies the ability to select the patients likely to benefit from certain cytotoxic agents. Changes in...

Introdução: A sífilis congênita é um grave problema de saúde pública, que causa índices elevados de morbimortalidade intrauterina, revelando falhas e fragilidades do sistema de saúde. Objetivo: Relatar caso de sífilis congênita em um hospital universitário da região centro-sul do estado do Rio de Janeiro, Brasil. Relato de caso: Gestante, com idade...

Rapid advances in molecular biology and bioinformatics have created a great need for the analysis and processing of genomic data. This need challenges current computational capacity, given the size and complexity of genetic information being processed by conventional computers. This paper explores how quantum technology can contribute to genetic in...

Over the last ten years, elements of the formalism of quantum mechanics have been successfully applied beyond physics in areas such as psychology (especially cognition), economics and finance (especially in the formalization of so-called ‘decision making’), political science, and molecular biology. An important stream of work along these lines, com...

ViralZone (http://viralzone.expasy.org) is a knowledge repository for viruses that links biological knowledge and databases. It contains data on virion structure, genome, proteome, replication cycle and host-virus interactions. The new update provides better access to the data through contextual popups and higher resolution images in Scalable Vecto...

Although the extraordinary progress has been made in molecular biology, the prevention of cancer remains arduous. Most solid tumours exhibit both spatial and temporal heterogeneity, which is difficult to be mimicked in vitro. Additionally, the complex biochemical and immune features of tumour microenvironment significantly affect the tumour develop...

Background: Fabry disease (FD) is a rare hereditary multisystem disease caused by variants of the GLA gene. Determination of GLA gene variants and identification of genotype–phenotype correlations allow us to explain the features of FD associated with predominant damage of one or another system, both in the classical and atypical forms of FD, as we...

This beginner’s guide to supervision has been created for anyone who supports postgraduate researchers (PGRs) with any aspect of their research or the completion of their degree. The supervision of PGRs is a complex and time-consuming job, with a high degree of responsibility. Good supervision is a key component of PGR success and is vital to the h...

Uterine fibroids is the most common benign myometrial tumor, which affects approximately 20% of women over 30-35 years of age [6,7], is diagnosed more often in late reproductive age [9]. Due to the fact that in some cases there is an asymptomatic course, the disease is detected in 5 - 10% during routine examinations or ultrasound examination [1,...

The microbiological diagnosis of pleural effusion is largely based on classical microbiology methods, but these methods have a high rate of false negative results. Some previous studies have shown improved diagnostic performance for pathogens such as S. pneumoniae using molecular biology methods. We present the use of a Multiplex-PCR platform (BIOF...

The study presents the results of taxonomic identification of mammals by molecular genetic methods. Fragments of dried soft tissues of animals found in their natural habitat in the republics of Altai and Tyva have been studied.