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Mineralization - Science topic
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Questions related to Mineralization
Dear N cycling community,
I have been looking for studies reporting the difference in the gross N mineralization rates in soils under oxic vs. anoxic conditions?
Lower rate is expected under anoxia I assume, but would anyone have any concrete numbers to this, or good hints about references? Also studies reporting mean residence time for ammonium under oxic vs. anoxic conditions would be helpful.
Best, Maija
Colleagues, at leisure I have read the literature on the strontium extraction (crown ethers, inorganic ion exchangers and resins and etc.). It was found an interesting fact - in most of the articles investigations were done at low concentration of competing ions (the biggest problem is calcium, if I am not mistaken) and low mineralization of the solution as a whole:
So, I am wondered: what about solutions with high mineralization (suppose more than 200 g/l) and the ratio Ca/Sr>10?
I'll be appretiate for the relevant links.
Hi
Me and my collegues found a strange rock aggregates here Lunda Sul, within Kalahari sand suite.
Similar formations (ferricrite, ferricrete) are found within Lunda Sul, Angola on the surface and in the strata of the Neogene-Paleogene red-brown sand deposits of the Kalahari as different unshaped fragments with different sizes.
I guess it could came from more ancient sedimentary rocks. I have attached some photos and description
So, we have these cylinders splited on 4 sector, 5 sector and 6 sector bofy filled by iron mineralization mixture (mainly composed by goethite, hematite, relics of fayalite, few inclusions of quartz). We have done only X-ray diffraction analysis and single thin sections, but wave microprobe have not yet been done. The spaces between sectors filled by Kalahari sands. Aggregate of goethite botryoidal form, porous filling, brownish dark-gray color, metallic luster, yellowish-brown streak, hardness 5, density about 4 g/cm3, slightly oxidized on chips, in voids and pores. Magnetic susceptibility about 0.16 SI*10^5.
I have one exotic idea about origin but with many questions.
It could be tree trunks, splited into pieces after drying or fire, filled with clay soil during wet season, which was then consolidated after next dry season or new fire. Subsequently the wood (or coal) in sectors were weathered and..... replaced (?) by iron-rich mineralization. Perhaps the processes of psephdomorphic replacement occurred in several stages. As a result, we obtained botryoidal goethite.))
It remains unclear the olivine origin in the botryoidal aggregate?
Why, instead of vertical zoning and the ring structure of the tree, we have, on the contrary, horizontal sinter structures?
What do you think? Please look at the concretes. May be you have seen similar or have idea what is it? Which signs and features I could check without any complex analytics??
Below attached images of aggregates:
if the fugacity of S rises or fugacity of O drops, is it possible to precipitate magnetite and pyrite from a same hydrothermal fluid? Is there any case study?
Recently, we have been encountering challenges in our laboratory in achieving satisfactory mineralization of MC3T3-E1 cell line. We would like to determine the optimal concentration of Ascorbic Acid and β-Glycerophosphate to achieve the desired outcomes. Additionally, we are unsure whether we should initially culture for 8 days with Ascorbic Acid only and then for another 8 days with β-Glycerophosphate only. We appreciate your assistance in resolving these issues and thank you in advance for your collaboration.
Kind regards,
Antonio.
Dear all,
What are the appropriate techniques to determine the mineralization of photocatalysis? Note that I do not have TOC
Recently, as I was studying some magnetite ores under polarizing microscope, I have observed some magnetite stars (I call them magnetite snowflakes!). Please see the attached picture.
I wonder if this form of mineralization could tell us something about the conditions of occurrence of this type of ore.
I appreciate comments or references in this regard from colleagues.
Kind regards and best wishes,
Nima
Structural features such as faults, veins, folds etc. can be observed in a granitic rock. How do these affect the mineralization of ion adsorption clay type of REEs? Could more fractures indicate enrichment of the elements or vice versa?
I want to know more about antigorite mineralization.
Hello, Dear scientific community,
I want to delineate hydrothermal alteration zones using an RGB Band combination on ASTER data. I've already consulted the literature on this topic and I found that 468 is the most relevant band combination for alteration and lithology discrimination. but I want to know if is there any mathematical method to calculate and select the most appropriate band combination.
I have been studied on ethanol effect on mineralization process of calcium and potassium in HEPES buffer and use plate reader to see the changes occurred while processing. When ethanol is added to HEPES-Ca and HEPES-K separately it does not affect the curve of absorption but when HEPES-Ca-K with ethanol come together mineralization curve do not match what I expected to see. What causes this confusing result?
Hello,
In our lab we routinely cryosection decalcified mouse hemi-mandibles on the sagittal plane and collect the sections on slides. We usually do this for immunofluorescence or in situ hybridization experiments, but we have recently started sectioning samples for H&E. Our IF/ISH sections are 10-30um, but we are attempting to section our samples for H&E at 7um for best staining/imaging. However, we are consistently getting folds, rips, tears, and wrinkling at 7um, especially at our area of interest (which is enormously frustrating). Sometimes we can manipulate the cryostat and block to obtain one or two decent sections, but is there something we can do to produce thin, flat, even sections? I have read through the IHC world cryosectioning guides many times, and have yet to troubleshoot this effectively. We are coming up on a deadline, so I am getting kind of desperate! Thank you in advance for any advice.
Hello to all researchers, can the supergene mineralization process exist in the beginning of mineralization? Or is the presence of hypogene necessary for the mineralization of the supergene?
The processes that soil microbes convert organic matter to carbon dioxide can be termed "decomposition" or "mineralization". The difference between these two words is still ambiguous to me.
I am working on dye degradation, I have got a total mineralization of the dye (Malachite green oxalate) but when studying the kinetics of degradation and trying to fit my experimental data none of the model (pseudo-first, pseudo-second, ...) has fitted with my data?
Thank you in advance.
Hi,
I am interested about how quickly SOM can deplete over time, and would like to start a discussion on the topic. Please pardon me if my question is broad.
In temperate systems, it is common to find annual decomposition coefficients around 1-3% (i.e., 1-3% of the SOM stock is lost after a year). However, I wonder how quickly can SOM mineralization occur.
While reading the literature on SOM changes after deforestation in the tropics, I found values suggesting that SOM stocks can decline by 10-50% in a few years (5-10 years) after a forest is cleared for cultivation.
Also, while looking at the AMG soil organic matter model, I noticed that the potential (maximum) SOM mineralization rate (k0) was set to 29%!
Have you ever asked yourself this question?
Related to this topic, I was thinking of a simple experiment that could shed some light on this question. Let's imagine pots with freshly collected soil or a plot of land, which is outside, and for which any plant development is precluded (removing seed, young seedlings manually). I would be curious to see how quickly SOM changes over time (considering that we would regularly monitor it or regularly SOM contents), given that no plant can inject organic matter. Of course, this soil would be exposed to environmental changes (such as regular water inputs from rain or manual watering, not to let it dry).
Any thoughts about this?
These are two rock types. The first one granite with Azurite and Malachite mineralization and second one is quartz and feldspar dominated rocks with Azurite and Malachite with pyrite.
Crystallization, HAP, Mullin, Nancollas, constant composition technique, supersaturation, labile region, pathological mineralization.
I am running an experiment with m3 t3t cells to study the osteoblastic differentiation and mineralization over 21 days. I was wondering how many cells I need to seed in each well of a 48-well plate to avoid overconfluency while having enough cells to fulfill the purpose of the study?
- Who has any clue from manganese mineralization in subduction setting or post-collisonal setting? if you have any paper about affromention subject please share here.
- Thanks all.
I would like to compare between the speed of the mineralization process between the naturally happen in the soil and the injected CO2 into basalt.
I am aware of the theory of CoDa and its importance in the analysis of environmental and hydrogeochemical data, where most of the variables, including major, minor, and trace elements and oxides, are examined.
But what about mineral exploration studies? We often adopt only a few metallogenic trace elements of interest that make a negligible contribution to the whole composition.
For intuitive understanding, I examined 11 case studies with some trace elements (6 to 10), and the disagreement in PCA results (with raw- and logratio-transformed data) was completely insignificant!
The question particularly considers the Variscan basement in Germany, the Czech Republic and Poland. A Late Pennsylvanian-early Permian quartz-hematite mineralisation is reported from the Mid-German Crystalline Zone (Kyffhäuser, Germany), but where else?
I have come across a newly prospected deposit, Low Sulphur Epithermal trending Alkaline. Au and Palladium PGE hosted in Ginguro Banded quartz. The nearby volcanism (rhyolite plug approx. 20km away) is bimodal, highly alkaline Basalt and Rhyolite. My question stems from a unique characteristic of the ore. The Ginguro Bands which host (very high grade) Mineralization, are paramagnetic. Has anyone come across this before? Can anyone help explain what mineral assemblage is likely at play? Or point to any relevant articles? Any help or insight would be greatly appreciated. Approximately 80% of the precious metals contained can be concentrated with simple magnetic separation. And the grades of the ore samples are very high.
Looking for the cooperation from geoscientists having laboratory access/facilities to analyze Oxygen and Sulphur isotopes in delta notation and zircon chemistry by LA-ICP-MS.
The above experimental requirements are a part of exploratory work for probing fertlity status of a newly detected porphyry style mineralization in NE Indian Precambrian shield having all basic signature of mineralization.
Is the age of mineralization of the deposit too old? How accurate is it? Is it related to craton destruction?
Dear all,
I have to mineralize milk samples for trace metal analysis
Really small amount are available so I would like to use the 64mg5 rotor of Anton paar microwave but all the litterature I found use larger vials
Do somebody have a protocol using mg5 vials?
Best,
Amandine
is there a relationship between the copper mineralization hosted in the Neoproterozoic basement formations and the other that is hosted in the Cambrian cover in all the inliers (Boutonnières) of the Moroccan Anti Atlas ?
How does metasomatism/alteration lead to berryllization with other oxides mineralisation
Is decreased % of organic carbon in residual soil compared to the initial soil is an indication of increased mineralization of SOC due to the addition of new organic matter?
Is the difference of 2℃ in soil temperature is enough to make a significant difference in nutrients uptake and mineralization?
what is the optimum soil temperature for nutrient mineralization and uptake for temperate fruit crops like blueberry?
I have planned to start a new research in soil fertility. Now I am looking for new areas to be addressed. Biochar or Organic matter mineralization have being suggested. I need a new area
Because of High C:N ratio of many crop residues it's decomposition rate is slow. In What way the c: n ratio is impacting the the process of decomposition , mineralisation of nutrients and it's availablity to crops.
In Mexico, there are canyons areas, where some examples of epithermal deposits, with Au-Ag mineralisation that shows Low sulfidation aspect (chlorite, carbonate, fluorite, amethyst, acantite, galena, sphalerite, chalcopyrite), were found above or next to Porphry Cu-Mo stockwork (QSP+ veinlets A+B+D type).
If you have observed such phenomenon, what is the location? If possible, what was distance between the LS epithermal deposit and the porphyry stockwork?
We want to make a compilation of similar examples and their geological setting in Mexico and the other examples in the world.
I would like to know and understand how mineralisation occurs.
Mineralization process increases nitrogen availability for plants in the soil. Both soil organic matter and microbial biomass are mineralized. Can we differentiate how much mineralization of these two processes contribute in nitrogen release in the soil? I will greatly appreciate all the suggestions and relevant literature.
Both methods are expensive and before doing
I'm currently trying to induce and identify mineralization in my SaOS-2 cells.
The issue I'm having is that the whole well will stain red, and of course this intensity depends on how long the cells are left in the Alizarin Red S staining solution. I look at these cells over a time course of 0, 3, 6, 9 and 12 days. I grow these cells in DMEM F12 Glutamax media supplemented with 110mg/L sodium pyruvate and 1% penstrep. SaOS-2 cells were seeded at around passage 10 +/- a couple of passages.
My current culture protocol is as follows:
Seed SaOS-2 cells at 50,000 cells/cm2 in a 6-well plate and grow cells in DMEM F12 Glutamax supplemented with 110mg/L sodium pyruvate, 10% FCS and 1% Penstrep.
After the cells have reached confluence and I wash the day 0 in PBS and fix it with 4% PFA for 5 mins and store the cells at 4oC in 3ml PBS. and then add osteogenic media to the remaining wells.
Oestrogenic media = DMEM F12 glutamax supplemented with 110mg/L sodium pyruvate, 10% FCS, 1% penstrep, 50ug/ml ascorbic acid and 5mM Beta-glycerophosphate (I've also trialled 2mM B-GP and 10mM B-GP and did not notice any immediate differences).
I then culture cells for 3, 6, 9 and 12 days in this osteogenic media washing and fixing them at their respective time points.
Alizarin red protocol:
Once all time points have been fixed, I wash the cells twice with DI water and then add 1ml of 2% alizarin red (pH ~4.2) to the well and I've tried this staining for 5 minutes and up to 30 minutes, I then wash the wells 3 times with DI water.
I tend to see an overall staining of the well with small red dots showing in the wells. I am of course looking for nodules as other papers have indicated however I still see a bit of background and the nodules are not as intense nor as large as you'd see in publications (so it would seem). The contrast between the background and the specific dots is not as clear as it is shown in publications.
Upon inspection under EVOS XL Core microscope there are definitely nodules of cells where the stain is gathering in a more intense manner, suggesting the staining is specific, despite the overall culture well looking red.
Many publications have great images with clear nodules and minimal background whereas others have less defined clear staining and I'm unsure which I should use as a guideline.
While culturing the cells I definitely see cloudy areas which indicates to me a collagen matrix has been synthesised, before staining the cells I also seen black-ish looking deposits which I think may be mineral.
I am also detecting collagen 1 and alkaline phosphatase at the RNA and protein level (Col1 protein increasing over the time course) and osteocalcin RNA expression increasing over time suggesting something is definitely going on.
Any information or advice would be appreciated.
Thanks,
David.
As MRT express the decay rate (average time that biochar can persist from decaying) and this rate decreases with time. Therefore, the incubation period becomes pivotal for the MRT calculation because shorter duration may lead to higher estimated mineralization rate and shorter MRT. So, can we consider other means of getting justifiable stability period?
please suggest a methodology for above.
Hi all,
i am looking for a way to quantify the Alzarin red stain of my FFPE bone slices. My problem is that i want to compare the development of mineralization at specific time points and my original bones are all different in size. So in my opinin I have to account for the size differnece, but I have no idea how to analyze this. Furhtermore, I have a lot of slices to analyze and it would be great if there is maybe a program which runs semi-automatic (after I figured out which thresholds are best).
Thanks for your help!!
Vanessa
Any research that explain about mineralization and metamorphism in pelitic rocks
1. Boundaries of Nile craton
2. Its Geochronology
3. Mineralizations
We are researching on polymetallic magmatic-hydrothermal related to mineralization. Meaningful contributions and supporting journals shall be grately appreciated.
Many thanks.
I have done a resistivity and induced polarization (IP) in a granitic area of generally S-type, tin bearing. The resistivity method detected a massive anomaly of >3,000 ohm.m which represent the granites of at least 40m depth.
But within the anomaly itself, a range of chargeability (17- 800 msec) did also appear.
What does contribute the chargeability values like that?
Could there any clayey minerals exist within the granites? Or is it related to any mineralization i.e greisen or sulfide in the hard rock?
How the re mineralisation of enamel is happening in avascular dental enamel
Is there any way to answer the question
Typically when I do in vitro osteogenic differentiation, I perform a protein assay as well on the cell lysate upon harvesting, and normalize the ALP activity to protein concentration.
However, I'm wondering if this method is a reliable measure of ALP activity per unit, especially if I have a scenario whereby I'm adding, say, rhBMP2 to one group's mineralizing medium, and have a negative control group that just has mineralizing medium. Wouldn't the higher intracellular BMP2 reflect a higher protein concentration that doesn't necessarily mean a higher cell count? Is there a way to normalize ALP activity per cell or comparable unit, other than counting the cells when harvesting?
Photocatalysis is termed by researchers (or green chemist) to eliminate secondary pollutant by mineralization of organic pollutants.
I have recently visited a mineral occurrence with the following general characteristics:
Pervasive argillic alteration in a subvolcanic intermediate rock, sulfide veinlets (partly oxidized), sulfide-bearing quartz veins, mineralized breccia, anomalies (or rather high amounts) of copper and gold, etc.
As a whole, this occurrence seems to demonstrate the general features of an epithermal and /or a porphyry type of mineralization.
An interesting feature to me was the presence of sets of parallel sulfide veinlets which in some cases, have been crosscut by other sets of parallel sulfide veinlets.
I wonder, if this type of texture could be considered stockwork? Please see the attached pictures.
I ask this, because some colleagues believe these are not stockwork because they are not randomly distributed in the rock.
I appreciate any comment or experience in this regard.
Which type of minerals are associated wih skarn mineralization? Is it possible to reveal skarn mieralization on basis of petrograhic studies of significant skarn minerals.
Certainly calcareous soils rich in micronutrients many times...is there any other method to access the potential of that soil to make those nutrients available?
Using factor and cluster analysis for mult element compositional analysis. How can one identify the group of elements associated to mineralisation, say, for example in PC of 5 factors (from the five factors with their individual elements. How can one identify the group linked to mineralization
Hello everybody,
We have recently started studying osteogenic differentiation in pericytes. Alizarin Red staining protocol works. The problem is that we are not sure if this is real specific staining or just some kind of background because it looks different from what we usually see during "true" mineralization in cultures of adipose-derived stromal cells and MSCs. There are no dark-red crystals, the staining is sort of uniform over the plate, with very strongly stained nodules. Maybe this is just non-specific staining of overconfluent areas, or...? Please have a look, any advice is highly appreciated!
I want to quantify the rate of mineralization of preosteoblasts (
MC3T3-E1) on a 3D scaffold. However, the scaffold is mainly composed of Hydroxyappatite (HA). Thus, I cannot use Alizarin Red Staining because HA is partially composed of Calcium.
Hello everybody,
I have two questions for the mentioned topic:
1) What is the lowest detection limits for these sulfides?
2) Which Fe- and Cu-sulfides show the best results based on Re-Os background values and DL?
I do appreciate your kind replies.
Best wishes;
Shahrokh.
Want to conduct a qualitative assessment of degree of mineralization of a mineralizing solution in SBF overtime with O.D measurements.
Hi, have any article about Carbonatite mineral system analysis and predictive exploration targeting?
I have 20years in geology of Phosphate Indutry in Brasil. I would like to know about your project.
It is possible?
Tks
I use 70% HAP as a mineral part of bone and 30% Carboxymethyl chitosan as a organic fraction of it but the final scaffold did not have suitable strength.
I need these file regarding my research
I am looking for k2 values: "humus annual decomposition rates"
for tropical (cocoa) conditions, in Indonesia.
I would like to use them for a simple Hénin-Dupuis modeling experiment on soil organic matter dynamics.
Otherwise, have you heard of a method to approximate the k2 value depending on climate and soil data for example?
Thanks
Hi,
How can we perform cleaning validation for a multivitamin multimineral formulation. Is there any regulatory guidelines which says about cleaning validation of this formulation? If so kindly share the same.
I am also not aware how it can be performed when we are adding the vitamins and minerals in a low concetration. Is it required to perform the analysis for all the actives (vitamins and minerals in it) or we can select the any one of the maximum concentration in the combination?
Is it mandatory to perform cleaning validation of a multivitamin mineral products if so kindly share the document
Please guide me as I cant find any answer about cleaning validation methodology of a multivitamin, multimineral products & the limits for the same.
Thanks in advance.
Can we avoid OsO4 formation during microwave digestion of food matrices while using a mix of HNO3 and HCl (for mineralization) ? FI, Osmium spiking is at 1ppm. Thanks.
Do you know the way to make a purchase of a pure muscovite (powder or sheet) via online? If you have some information to get the mineral, please let me know. Thank you in advance.
I am looking for books regarding the tools and methodologies for the geophysical mapping of seafloor mineral resources, especially seafloor massive sulfides.
Hi !.
I have heard a lot of Skutterudites (materials or the mineral structure) for its use in Thermoelectrics ,
but, I want to know : of the seven Crystalline Systems ; Cubic, Hexagonal, Monoclinic, etc. Which one is the form of the Skutterudites ?
My team is evaluating the San Luis del Cordero project in central Durango State, Mexico. This is a quartz-feldspar porphyry intrusive, which is in contact with calcarious shales and limestone units. There are both skarn zones and epithermal veins associated with the intrusive. I welcome any suggested references for this type of mineralization.
Are P solubilizing bacteria moisture sensitive (aerobic or anaerobic)?
I’m designing an experiment to analyze gross N mineralization and nitrification rates in tropical pastures using 15N dilution technique. These fields have all very high root density and strong aggregation, it is Vertisol. Under such conditions, is it advisable to use intact soil cores or would it be better to use disturbed soil, remove the roots and then apply the 15N label? Also, is it better to use disturbed soil and sieve the samples prior the 15N application or use intact soil cores and then sieve the soil before the 15N extraction?
Thank you very much in advance
Hi everyone, happy new year!
I'm performing a osteoblast differentiation assay with C2C12/BMP and I was wondering if it is possible to do mineralization assay, besides Alkaline Phosphatase assay, to characterize C2C12 differantiation?
Many Thanks
Hi,
I have heard many times that :
With no-till, organic matter content tend to be higher than in conventional systems.
And that :
With no-till, mineralization rates are lower tend to be lower than in conventional systems.
From this point,
Has it been demonstrated that in no-till, a higher content of organic matter can compensate the diminished mineralization rates, thus leading to a higher availability of nutrients at the end of the day ?
(considering that we compare a field with the exact same settings, the only changing factor is ploughing).
I enclosed the two equations, capture and capture 1 for the reference