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Migration - Science topic

An interdisciplinary group for research on migration issues.
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I am doing a Transwell experiment to look at monocyte migration. The cells are migrating to the bottom of my plate and some adhere some are suspended. I would like to count the cells without removing them with accutase or scraping to avoid losing cells. I have looked at CellTiter-Glo as an option. Are there other options that work better?
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You could take images of the transwell insert and use ImageJ-based automated method for rapid quantification of cell number. Please refer to the articles attached below.
Best.
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I am an international student and I am studying sociology at McGill University.
I am interested in the sociology of immigration (in particular, immigration in Canada) and have read research papers about this topic. However, I feel I'm stuck generating possible research topics for my final Master's research paper (it will be 30 to 40 pages long).
Does anyone have any exciting immigration study subjects in Canada for a Master's research paper? I am looking for both qualitative and quantitative research topics.
I also would appreciate it if you could introduce any interesting papers about immigration, mainly, in Canada
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(PDF) Immigration: analysis, trends and outlook on the global research activity (researchgate.net)
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I'm doing a research on migration process and history of migration and looking for scientific specific data about what kind of challenges migrants can face in foreign countries
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The life of a refugee is hard, they’re fleeing places of war, hunger, famine, persecution and have gone through a tough journey to find a safe haven for themselves in a completely different journey.
What challenges do Refugees and Migrants face? – Epimonia
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Hello,
I am working on the immune response in the respiratory tract 30 minutes after a stimulation, and I'm also analyzing the bone marrow. I observed a rise in the number of Lin-, MPP and CMP, but I'm wondering if 30 minutes of stimulation is enough to influence the BM . Does anyone know, how long it take for progenitors to "sense" the inflammation, to differenciate and to migrate ?
Thank you very much !
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Hi Mahana,
Possibly, obviously the pathways are very complex but if you have mature APCs they would initially move to the nearest lymph and generate a specific humoral response which usually takes about 96 hours after stimulation and more than likely depends on the stimulus still being available. If the stimuli is there for ca 30 mins in the lead up to a more adaptive response and of course any sort of plasma cell or b cell proliferation then it may be too short as innate cells (such as macrophages) in the area in the lack of stimuli will apoptose leading to an increase in IL-10 and down regulating the immune response in the area and secondarily in the lymphatic system.
I think it may be enough to start plasma cell and B cell proliferation but may not be enough to continue the process and yield cells to fight the "infection".
It might be a different story if you repeatedly stimulated for 30 mins (e.g. every 24-48 hours) the times are just a random suggestion but I think repeated exposure within that 96 hour window may elicite more of a response as the antigen will still be present and presented on MHC receptors for example.
If it does stimulate a response in the bone marrow, I would be interested to know how they mature. If they fully differenciate and mature, I would expect with a short exposure that it may start but the stimuli may be too short if it starts at all.
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Hi there,
searching some sort of UNHCR with scenario analysis. Cherish your feedback.
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Hi everyone!
I would like to know if you have experience with migration and invasion assays using transwells (boyden chambers). If I use type I collagen coated trnaswells, is that migration or invasion assay? I thought only with matrigel it was possible to asses invasion but if I block the pores with type I collagen and then measure the migratory cells, in fact, I'd measure the cells with the ability to degrade collagen in the pores and then migrate, right?
All comments are welcome :)
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It's an invasion assay . Invasive cells secrete proteases that degrade the matrix ( Matrigel or Collagen ) and enable invasion .
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People migrate for many reasons. These reasons can be classified as economic, social, political or environmental: economic migration - moving to find a job or following a certain career path.
Migration can expose hosts to a greater number of infectious diseases, as it covers a larger area and visits more habitats than residents. However, because long-distance movement is energy consuming, migration can have a devastating effect on infected hosts, reducing the risk of infection.
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Greater human mobility, largely driven by air travel, is leading to an increase in the frequency and reach of infectious disease epidemics. Air travel can rapidly connect any two points on the planet, and this has the potential to cause swift and broad dissemination of emerging and re-emerging infectious diseases that may pose a threat to global health security.
Population migration, spread of COVID-19, and epidemic prevention and control: empirical evidence from China | BMC Public Health | Full Text (biomedcentral.com)
Human Mobility and the Global Spread of Infectious Diseases: A Focus on Air Travel (nih.gov)
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What type of models are recommended to show the relationship between the migration behavior and the socio-economic status of in-migrants?
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Migration is considered to be one of the important strategies for the progress and development of rural poor. People migrate from one place to another for the development of their overall living standard and to enhance their social position. Social survey technique and Focused Group Discussion (FGD).
(PDF) Internal Migration and Socio-Economic Status of Migrants: A Study in Sylhet City, Bangladesh (researchgate.net)
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In the legal field of custody pending deportation (Abschiebungshaft) in the Federal Republic of Germany, errors happen more frequently in the lower instance, the district court. Above all, it is procedural errors that lead to decisions later being found to be unlawful, for example, a lawyer who can be seen from the files is not contacted or there is insufficient language mediation. In terms of reasons for this, the lack of time and the density of court proceedings are often put forward first, leading to a lower qualitative examination. In the same way, legal ignorance can sometimes be observed in the legal field of custody pending deportation.
I am interested in whether there are other areas of law in which similar errors can be identified at the lowest level? In other words, procedural errors that happen again and again.
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Are there any instructive and recommendable studies about the preunderstanding of a judge?
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hello everyone
I'm writing scenarios with fsc26
however, I have trouble filling the sample sizes in tpl files
so far I just fill the sample sizes with *2 of my empirical sampling sizes (cause my focal species is diploid so pop0=181*2, pop1=24*2)
however, these sample sizes tremendously increase the computation time to almost 1 month (I'm using Linux with 10 cores for each model)
to save computation time, will the estimated parameters be strongly affected by the smaller sample sizes I give in my scenario (e.g., only use half the original sample sizes)?
thanks a lot!
below is one of my tpl and est files of an early migration model
//Parameters for the coalescence simulation program: fsimcoal2.exe
2 samples to simulate :
//Population effective sizes (number of genes)
NEW
NHC
//Samples sizes and samples age
362
48
//Growth rates: negative growth implies population expansion
0
0
//Number of migration matrices : 0 implies no migration between demes
3
//Migration matrix 0
0 0
0 0
//Migration matrix 1
0 MIG1
MIG2 0
//Migration matrix 2
0 0
0 0
//historical event: time, source, sink, migrants, new deme size, new growth rate, migration matrix index
2 historical event
TNOMIG 0 0 0 1 0 1
TDIV 1 0 1 RESIZE0 0 2
//Number of independent loci [chromosome]
1 0
//Per chromosome: Number of contiguous linkage Block: a block is a set of contiguous loci
1
//per Block:data type, number of loci, per generation recombination and mutation rates and optional parameters
FREQ 1 0 1.8e-9 OUTEXP
// Search ranges and rules file
// ****************************
[PARAMETERS]
//#isInt? #name #dist.#min #max
//all Ns are in number of haploid individuals
1 NEW logunif 10 1e7 output
1 NHC logunif 10 1e7 output
1 NANC logunif 10 1e7 output
0 MIG1 logunif 0.00001 1 output
0 MIG2 logunif 0.00001 1 output
1 TNOMIG logunif 10 1e5 output
1 TPLUS logunif 1 1e5 output
[RULES]
[COMPLEX PARAMETERS]
0 RESIZE0 = NANC/NEW hide
1 TDIV = TNOMIG+TPLUS output
by the way, this model will stuck sometime and I have to re-simulate the model, could I edit something in this scenario to avoid stuck? thanks for any suggestion!
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Cause no one is answering, I will share a paper someone shared with me recently
The samples sizes In this paper is 20-40 among models, however, it seems to work quite well for estimating parameters
So maybe it is acceptable to use small samples sizes using fastsimcoal2
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I hope to have any explanation about migration of RNA, when i finish extraction i make electrophoresis in TAE buffer with 1% agarose but the result (no bands of RNA) but after a few days when I repeat the elctrophoresis the bands are present, what's the wrong ? and I hope to have some advice please to get a good migration. Many thanks
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The obvious answer is as Asmita and Marisol mentioned, just making sure that ethidium bromide was included in the gel, but if that isn't what happened, consider the following: RNA can be slow to go into solution, and sometimes after sitting for a while or after freeze-thaw it will be solubilized and the apparent concentration can increase. Also if the TAE used was old it may contain RNases from microbial contamination. Was it fresh TAE or a different batch of buffer the second time? Did you measure the concentration of your RNA by UV spectroscopy? It would be helpful if you could share that information and an image of your gel.
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Dear all,
I am trying to over-express some me the NaPi2b membrane protein in HEK293 cells to later run some Co-Ip. The protein has a DDK tag and seems to be expressing as I can detect a strong band at the top of the gel with a DDK antibody. The problem is that when I run my Western blot to check it it expressed the the protein seems to stay at the interface between the stacking and the resolving gel, and does not migrate to where it should be at around 76KDa. So I cannot be sure that it is expressing correctly. I have tried some methods mentioned here, where they suggested not to boil the samples before I run the gel and got relatively better results never the less there is still allot of protein stuck at the top. The gel seems to be running ok as GAPDH migrates to its correct place. I am using a RIPA buffer with protease inhibitors and my no transfection controls don't have this band so I don't know what exactly it is I am doing wrong.
I the picture picture you can see the strong band at the top of the membrane and the correct band at the 76 KDa where the protein should. The upper membrane is one in which I did not heat the samples and the lower membrane was with heated samples . Is these anything I can do to solubilize these proteins? What am I doing wrong? Thank you so much for your help
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Dear Andre,
do you check the pH of your samples before transfer on the gel (after adding the loading buffer and boiling)? In the case of the problems with electrophoretic mobility in the gel, I can advise using the gradient gel (for example 4-15% or 4-20%). There are dedicated primarily for the proteins with high molecular weights, but maybe for your protein also will be good.
Good luck!
Chris
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Hi guys!
Sorry for bothering. This is Haochen, MA student from Royal College of Art, London, Design Futures. I'm working on a project on fishing and hunting communities in Bangladesh in 2050, and it's looking at the future impacts of global warming on the lifestyle of local fishermen. But I now have some confusions and may need more professional advice and guidance. I sincerely hope to get feedback and comments from you, which will be critical and precious to my project. Thank you so much for your patience and time to read this question!
My research focuses on the effects of freshwater river salinization caused by rising sea levels. After watching some documentaries, news, I realized that the problem of salinization of freshwater not only seriously affects the stable food and economic sources of many people (migration and extinction of many fish species), but also directly affects their health (access to drinking water). Finally, I would tell a series of stories of Bangladeshi fishermen in 2050, to show what their dilemma could be, what their future lifestyle would be like and what tools they would use under the influence of freshwater salinization. But now I'm missing some deeper insights to enrich my story, so I'd like to sincerely ask you some questions. thank you very much!
The following are some of my confusions:
1. Regarding fishing: If in the southwest coastal rivers of Bangladesh, will the salinity of the rivers change significantly in different months of the year? If so, how much change could be happened? Does this monthly salinity change depend not only on monthly precipitation, but more on local geological conditions? And will this change radically affect the migration of fish and thus affect the harvest of fishermen?
In 2050, with the possibility of more extreme weather, is it possible that seasonal or monthly changes in the salinity of a river will be more dramatic than today, forcing fishermen to move more frequently to maintain a stable food source? Suppose that the middle section of the Pasur River has a salinity of about 5 in the rainy season, and in the dry season, the salinity of the middle section rises to 20, causing many low-salt-tolerant fish to leave or migrate, while saltwater fish are not adapted to inland channels environment, so there is no corresponding immigration from brackish fish, which causes fishermen do not have enough fish to harvest?
Does the salinity of a river increase uniformly from upstream to downstream? Is the salinity change of the river water regular or predictable every month and every year? Can we help fishermen have a more stable source of income in different seasons by measuring the regularity between river salinity and fish migration? If this is possible, what data could be measured?
2. Regarding population migration: If the river water in southwestern Bangladesh is salinized, will the local soil be salinized accordingly, making the arable land uncultivated and the groundwater undrinkable? Is this likely to lead to a large-scale migration of large southwest populations to live in the freshwater areas of the north? Combined with the question regarding fishing, is it possible that fishing and hunting groups living on migration will increase as a result?
These are the questions I am confused about, thank you very much for your patience! I would appreciate so much if I could receive your reply and guidance. Wish to have further opportunities to learn more in-depth and detailed knowledges from you guys, thank you very much!
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Babita Mehta Thank you so much for your reply, Ms. Babita.
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Dear distinguished colleague,
Recently we have started comparative global research that we have started recently on 'Students' perception on the Russia-Ukraine war 2022' (link to the website: http://www.covidsoclab.org/russia-ukraine-war-2022/), covering various economic and social effects of this war. The global comparative analysis helps us formulate the most useful recommendations for policymakers.
If you are interested in participating (as a contact person and a potential co-author of a joint paper, do let me know to give you further guidelines – see also research guidelines on the webpage: http://www.covidsoclab.org/russia-ukraine-war-2022/research-guidelines/). Your main task at this stage would be to motivate students from your institution (or wider in the country) to complete the online questionnaire by 30 April 2022 at the latest (here is only a preview link: https://1ka.arnes.si/a/60ee60a0&preview=on). When we have the results, we will analyse and compare them (between countries included in our study - then is a plan to prepare academic article(s) relating to different (e.g., economic and social aspects) of the Russia-Ukraine war 2022 together with the analysed results of our questionnaire survey). You will also receive data from your country/institution in order to deploy it in further research. The detailed dissemination plan will be finalised later according to the interests of international partners.
If your time is limited and do not allow you to fully join at this moment, we would kindly ask you if you could motivate and share a link with your students to fill out the questionnaire (please, do see a message and a link for students below) and we will be happy to provide you with the data/result/report for your institution.
Please, do not hesitate to contact me in case of any further queries.
Prof. dr. Aleksander Aristovnik
CovidSocLab
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Interested to participate in the survey on perception on the Russia-Ukraine war 2022.
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Hello everyone,
I was performing migration assays using wound healing and transwells. I had different results in both analysis. Actually, with transwells I got significant results but by wound healing assays there is no difference between control and experimental. Is it possible to have these results?
I have read about different types of migration (collective and individual) but I do not really understand it.
Could someone tell me how these types of migrations work? and how is it measured by different assays?
Thank you in advance :)
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German Vega Briones what is this?
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Usually, sample buffer comprises 10% SDS, however, if it is reduced to 4-5%, does it affect the protein migration in gel?
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Thanks a lot! @Nazlican and Kousalya
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Essentially, we have various HCC cell lines that we plan to do a migration assay using corning transwell 8.0 um pores w/ matrigel. Utilized a chemoattractant of 5% FBS/DMEM serum as the chemoattractant. Utilized some growth factor + inhibitor that was present in other wells for variation. We seeded 50,000 cells and they were visible in the insert following seeding. However, when I washed/scrubbed the insert following fixation (4% PFA, then 1% crystal violet soln). The issue comes when I observed the cell/inserts under the microscope for imaging. I noticed that there were only cells present around the edges of the insert..My assumption is during the scrubbing with the cotton swab, the cells were removed. In this assumption, I would then assume that the cells never migrated down through the pores. For the chemoattractant with the growth factor, we used a concentration of 50 ug/mL and on the last variable, we used an inhibitor following that pathway with a concentration of 1.0 uM. The cells were allowed to migrate for ~24 hrs. I suppose, we could increase invasion time to 36 hrs to see if that works, but not sure what else to troubleshoot, as scrubbing is necessary to remove non-invaded cells..
Thanks in advance for the assistance.
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The result you found could be due to several factors:
1- Have you optimised the "matrigel" concentration through which the cells invade?. Too much of that and none of your cells will pass, which may explain why you are seeing cells around the edges where the concentration of matrigel is usually less.
2- The medium you are using to attract the invading cells could possibly be the reason.
3- The duration of the experiment maybe too short for this type of cells, which may need a longer duration to invade. I suggest you review references about needed time for HCC to invade.
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I have timelapses of migrating cells and want to analyze the behavior of the the cell processes, (like extension and retraction) as well as look at their length and branching etc. What software could I used best to do that? Any automated software available?
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cloud computing
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You can use Cloudsim simulator is suitable such that purposes
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Can the crosslinking of preoteins, intra or intermolecular, change the electrophoretic migration behaviour of proteins on SDS-PAGE so extraordinarily, that they migrate much lower then estimated? how to explain? higher globularity of crosslinked proteins ?
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Please refer to the article attached.
Best.
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The concept of immobility is showing up -- and often in relation to the closures that the pandemic has created. My concern or interest is in the way immobility is used. Often it seems posited as the opposite of mobility (or in other words NOT migration) and that is rather narrow. Immobility is also used to describe the limits on peoples who would otherwise cross national/international borders. Shouldn't internal movement factor into our discussion as an alternative? And immobility is more than not migration, would be interested to hear thoughts on this.
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Max J. Stein , yes, it does show up in discussions of social mobility (immobility) but in migration - it is often approached as what people DON'T do, and while there are links between mobility and immobility, NOT migrating is not the same as being immobile, it has to be more than a physical movement defined by a relationship to a geographic point in time as both Nicolas Parent and Neema Ghenim argue. At an institutional level, if immobility is referenced as NOT mobility, we lose the complexities that drive immobility as a household decision for example.
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I'm working on a Research focused on this theme and I would be grateful if some of you can share his/her knowledge about the topic. Any input is appreciated, thank you.
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You are welcome!
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Aim of qualitative research study is to elucidate the migratory push factors determining locally born and educated registered nurses to migrate from the only tertiary level hospital located in the small island, modern metropolitan capital city. in a country maintaining fairly stable economic and political conditions..
Undoubtedly, in consideration of globalization, an escalation in the number of native RNs migrating from a ''destination nation' for nursing migration, has grabbed the attention of healthcare systems stakeholders in the midst of the COVID-19 pandemic, there is no empirical evidence available to provide answers toward the planning and implementation of policies and strategies to stem the flow of these limited human healthcare resources' out of the nation's public healthcare system.
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Migration of labor like healthcare workers depends on several pull and push factors
Pull factors are better opportunities available at the destination sites for career advancement, better remuneration at the destination sites, job security at the pulling site, presence of high technology to make the work more fulfilling and enjoyable, adequate holiday periods for workers at the destination sites, higher and better pensions at the pulling sites and many others.
The push factors includes poor working environment, lack of technology, poor pension, absence of job security, comparatively low salaries etc. are but a few..
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Can anyone help me to formulate my research question for a thesis paper? I would like to do a research regarding the influence of sustainable development agenda 2030 on migration flows and human rights. Yet I also want to include in my research the fact, that not only safe and regular migration can help to achieve sustainable development, but the agenda 2030 can also have a positive impact on regular migration and human rights security (may be with a reference to gender issues or climate refugees).
I will be grateful of you could help me out!
Thank you!
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How migration contributes to local economic growth?
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What migration has to do with it?
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Richa Dobal Read it. You will get the answer.
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What do you think is the general framework for analysing migration? What are the goals and objectives of this analysis? How are migration patterns best categorized? What is the practical application of migration models?
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I'm looking for a questionnaire or survey the focuses on "reason for migration". I know there are questionnaires that include "reason for migration" items, but they tend to be focused on acculturation or some form of trauma.
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Dear Patrick, how will you handle the problem of honesty? - Some refugees do not tell the truth about their motives to migrate. Sometimes they even hide their nationality and pretend a certain kind of persecution.
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Hello. I am looking for a good choroidal endothelial cell line to
1- knockdown
2 - perform proliferation and migration assay.
I would prefer a murine cell line as I will also incubated these cells in conditioned media obtained from mouse microglia.
Thank you
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Hi Amos,
Thank you so much for your answer. I have missed these cells myself. I will considered them for my research.
Best regards,
Hélène
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I am interested in research migration to Poland and would be happy to read.
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You may want to look at https://theconversation.com/belarus-border-crisis-with-poland-loses-sight-of-the people-trapped-in-the-middle-171549
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Is there any centralized database that would list and preferably also shown on a map all Eurasian and African archaeological sites from years 5000 - 1500 BCE? I would need to see, whether the appearance of archaeological sites correlates with the appearance of ancient place names.
Our team studied the geographic distribution of 15 ancient name sets to see, where they appear as place names and personal names. We found out that most of the ancient name sets seem to have their urheimat in India (e.g. Phoenician, Hyksos, Mitanni, Berber, Sea Peoples and Ethiopian) or have a very strong connection with India. The second highest density of ancient names was found in the river valleys of Volta and Niger in Africa, with relatively high density also along Danube and at the western parts of Eufrat & Tigris.
A short summary of the study is available here:
and the full study is found in here :
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I suggest you the following books:
Clark, Peter (ed.). The Oxford Handbook of Cities in World History. Oxford: Oxford University Press, 2013
MacEvedy, Colin. The Penguin Atlas of Ancient History. New York: Penguin Books, 1979.
MacEvedy, Colin. Cities of the Classical World An Atlas and Gazetteer of 120 Centres of Ancient Civilization. New York: Penguin Books, 2011.
Maisels, Charles Keith Maisels. Early Civilizations of the Old World. The formative histories of Egypt, the Levant, Mesopotamia, India and China. London-New York: Routledge, 2005.
Woolf, Greg. The Life and Death of Ancient Cities: A Natural History. Oxford: Oxford University Press, 2020.
Yoffee, Norman. Myths of the Archaic State Evolution of the Earliest Cities, States, and Civilizations. Cambridge: Cambridge University Press, 2004.
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I am studying the annealing effects on double perovskites. As we all know, annealing shows some significant effects on decreasing defects and increasing grain boundary, etc. However, ion migration is always a daunting problem towards the stability issue of perovskites, which can be aggravated with high temperature and open-air environment. There are some references mentioned mechanical degradation issues under air environment. I am wondering if annealing under open-air is a double-edged sword. It may improve the crystal ability or degrade the sample due to ion immigration. Thanks in advance!
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Dear Da Cao many thanks for sharing this very interesting technical problem with the RG community. For providing you with a qualified answer it would certainly be helpful to know which kind of perovskites you have in mind. I assume that you mean the highly popular tin- and lead-based perovskite salts. In this case, there is a good number of relevant literature references about ion migration available. Please have a look at the following potentially useful articles dealing with annealing effects:
Annealing effects on high-performance CH3NH3PbI3 perovskite solar cells prepared by solution-process
Unfortunately this paper has not yet been published as public full text on RG. However, three of the authors have RG profiles. Thus in case that the paper is of interest to you, you can easily request the full text directly from one of the authors via RG.
Good luck with your research and best wishes, Frank Edelmann
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Have controlled migration of plants (various species of trees with a selective chosen genetic origin) can be a way to stabilize the forests of economic use and provide a fully renewable source of wood raw material? I recently watched the movie “Breaking Boundaries: The Science of Our Planet”, which organizes a lot of information about our Earth (official trailer - https://www.youtube.com/watch?v=Gb6wQtNjblk). Breaking Boundaries tells the story of the most important scientific discovery of our time - that humanity has pushed Earth beyond the boundaries that have kept Earth stable for 10,000 years, since the dawn of civilization. The forests are one of the systems that regulate the state of the planet. If deforestation goes above 25% of the world’s forest cover (we broke this estimated border), with global warming increasing, we are likely to experience an irreversible process of savannization and desertification. Can we help forests by accelerating the controlled migration of trees adapting them to climate change, without forgetting the role of the economic forest and biodiversity? Is our knowledge sufficient to effectively do it? What do you think about it? Is it worth intensifying research from this scope?
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This is what actually happens now in some countries (i.g. Denmark), they started afforestation with tree species from 'warmer' parts of Europe or from Asia.
I recommend you COST action: http://www.pen-caforr.org/
which is focused on creating climate adaptive forests.
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  • Does normative legal philosophy also have a potential critical function vis-à-vis existing, empirically provable injustice where the injustice is not so much promoted or brought about by discriminatory laws, incorrect court rulings or actions contrary to human rights in the sense of an ideology, but rather by legislative and political laissez-faire or even omission (cf. e.g. mediterranean migrant crisis, anthropogenic climate change or pandemics)? From my point of view, this should be the case (but where is it explicitly stated and conceptually discussed?).
  • Which concepts from the field of normative legal philosophy/ legal ethics could be used to transparently and rationally criticise such state and supranational omissions from a normative perspective? Should new concepts of legal ethics be developed, can existing concepts be adapted? Who are the primary addressees? From my point of view, the minimum connection between law, serving as the basis of state action, and justice, which can be assessed against Radbruch's formula, enables a normative evaluation of state and supranational omissions, but also provides the contours for corresponding (political) duties to act.
What is your opinion regarding these issues?
Some legal philosophical approaches to these questions can be found in my paper "Extreme Wrong Committed by National and Supranational Inactivity: Analyzing the Mediterranean Migrant Crisis and Climate Change from a Legal Philosophical Perspective", Göttingen 2021.
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I believe legal theory is a tremendous force in the identification of those gaps in legislation. I see legislators all over the planet engaged in the erosion of democratic processes because they are trapped into their own epistemological limitations. Fundamental rights are more than ever under siege, and to move forward into producing a legal theory that identifies the limitations of what has been done so far is badly needed. The functional disconnect between the mandates of international law and national realities is blastering.
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I am still in the early stages of this, however I am thinking of exploring the potential football has, as a means to aid the integration of Venezuelan refugees in countries like Peru and Columbia. I am reading that similar to Europe, refugees are subject to racism and resentment. While also in Europe, football clubs have started to partner up refugee forums to help. UEFA recently wrote a report of their commitment to helping the cause. I also wonder if CONEBOL could implement the same action. Early indictors suggest their is a gap in the literature, when it comes to refugees and football in South America.
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Dear Dr. Ben David Anthony Bird, As a topic for a potential PhD, I think it's a great idea. Currently there are already footballers in Serie A.
To get an idea, take a look at the following page:
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Hello,
we are running sds-page electrophoresis gel of two purified protein. Both proteins have the same theoretical size but in the gels they migrated differently. Both have different Pi, one is 6 and the other one is 7. Do you think that this could affect the migration?
Thanks.
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I have observed differences in migration of the protein in SDS-PAGE just after phosphorylation, so many post-translational modifications may influence that, I guess.
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What kind of relationship can be found between the governance of processes of migrants´ integration and local/regional governance of shrinking towns? Can migration and shrinking areas (regions) be a "happy couple"? What is the role of governance in for integrating migrants in shrinking areas in Europe? What mode of governance suits better? What are your thoughts and state-of-the-art litterature to tackle these questions?
In your opinion, governance is a solid theoretical framework that stands by itself?
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I understand that true integration is done from social cohesion. To achieve social cohesion, you must start at the beginning: kindergarten and elementary school. This is the first link in social integration / cohesion, where people get to know each other, understand each other and then help each other. I have something written (in Spanish) about it, you can see in the following links:
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I want to start a new research on the flows of migrants from the Middle East to Europe and I am thinking of using the gravity model to analyze it. Gravity models are frequently used in the analysis of foreign trade flows.
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Mojtaba Shahbazi Yes, there are many uses for this model, especially in the economic field...in international trade...migration...tourist flows. We take inspiration from physics to make our research more accurate.
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I am trying to relate Climate issues with migration and gender marginilization. I am wondering if there are any technical calculations or index that has been used before to quantify or measure the impact - the parameter may be economic, or any other. Depending on how it can be quantified, I would shape my research.
I am open to ideas
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Water and climate-related migration intersection with UN Sustainable Development Goal(s) agenda mainly: SDG 6: Clean water and sanitation, SDG 5: Gender Equality and SDG 16: Institutions and Policies and SDG17: Partnership models.
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What is the best scale to measure intention to leave the country? Is it a good idea to adapt some of the intention to leave the company scale?
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Thanks a lot :)
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Does some gene mutation that increase migration necessarily increase invasion?Is it possible that some mutation that enhance cell migration but inhibit its invasion?
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Migration is a prerequisite for invasion. Non malignant cells can migrate without being invasive e.g. neutrophiles. Invasion requires the ability of digesting sorrounding tissues. This requires the activation of metalloproteases and other proteolytic enzymes. In turn this requires an acidic extracellular matrix. As you can see, everything is interrelated.
Migration can occur without invasion. The contrary is not possible.
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Apart from the DSAS tool, in GIS what are the other options available for mapping and analyzing the shoreline migrations patterns over a long time period?
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Let me suggest the following:
1- Extract or digitize the reference or baseline shoreline manually at a particular date.
2- Run edge detection on the next image at the following date from the reference image.
3- Use the digitized shoreline to search for the nearest edge points that were obtained in step 2. It is some sort of iterative closest point search.
4- Select or develop a criteria to verify the similarity between the two shorelines
5- Then repeat the process for the rest of the images.
Indeed, the basic assumption is the existence of shape's similarity between the different shorelines at different dates.
4-
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The value of migration period i.e. "p" has been set to 5/12. Is there any specific reason behind the same? Why can't we use the migration period as 7/12 when the butterflies are in Land2 for 7months and in Land1 for 5 months. Or is there any other value that we need to take according to our problem statement? How can we decide this?
Same is the case with BAR value and maximum step value.
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Python sir
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In connection with the warming of the Earth's climate, progressive global warming, some areas located in the sub-tropical zone in the future will not be fit for existence, and the migration processes of the population will be intensified.
Do you agree with my opinion?
Please reply
Best wishes
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El calentamiento global es un fenómeno físico q puede ocurrir al deterioro de la capa de ozono. Los científicos y expertos buscaran una solución para conservar la vida en el planeta Tierra. Por ahora debemos protegernos de los rayos solares y el cuidado del medio ambiente.
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Hi everyone,
i'm writing my thesis on bilateral migration flows using a Gravity Model and I'm new to the topic. I'm using country and yearly fixed effects. Can I include a control variable on distance?
Thank you.
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He means the gravity model of internacional trade folks, its an economic model.
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Hello
I plasma(H2N2) treated Al2O3 plate (edge is connected to ground) and wondered if oxygen vacancy migration on the plate can occurs as electrons escape through edge.
Or whatever positive charge migration.
thank you
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This is a very interesting approach
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How do you evaluate the European Union's position on Refugees? Is the EU fair enough? Can irregular migration be prevented?
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Economic and financial crises, military crises and the growing climate crisis occurring in developing countries and the lowest income countries, advancing unfavorable climate changes and the effects of these climate changes, such as the more and more frequent droughts and pest infestations on farmlands, drying out wells, and soil sterilization, the decline in the biodiversity of natural ecosystems, etc., are key factors that will result in an increasing scale of international population migration. Therefore, it is necessary to develop social welfare systems for refugees, support in adapting to new realities, develop social solidarity, accept and tolerate other cultures and value systems, etc.
Best regards,
Dariusz Prokopowicz
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Image Citation: Srinivasan, A. (2020, March 30). As Migrants Trudge Out of India's Cities, the Stark Realities of Migration Stand Exposed. The Wire. https://thewire.in/labour/as-migrants-trudge-out-of-indias-cities-the-stark-realities-of-migration-stand-exposed.
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The second wave of variants has affected both urban and rural areas intensively.
The impact of COVID has a tremendous effect on the three categories (Low, Medium, and High) of the standard of living of the well-being of society.
The respective State and Central Governments have recommended staying all the members in the house only. So there is no opportunity to do any productive economic activities during this pandemic period.
Except for the Merchandise and Medical professionals that they don't have any job in the outside environment.
The rest of them have stayed at home without any work, but some have really worked to do and the situation did not allow them to work.
The country's GDP drastically fall into this pandemic period. All the savings from the individual to Government will help during this period.
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Since last year the work from home and e-learning environment have proved to be some of the efficient ways of communication. The recent covid crisis has resulted in economic hardship for people of all ages. People are looking for opportunities of cost cutting. Migration from large metropolis is one of the recent moves observed in large cities like New York, San Francisco, Los Angeles, where people are moving to the areas in Idaho, Indiana and Oklahoma to minimize the cost of living including rent and mortgage. Is this trend observed worldwide?
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The people are migrating from costly cities to medium level states to have nominal household consumption expenditures.
The affordability has declined and purchasing power of the individual
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We know that band bending decides either electrons or holes can migrate to the surface, but how do we know which direction does the band bends (or it doesn’t)?
Then, why would the band bending change when the fermi level changes?
It would also be great if you only give some key words for me to search.
Thanks for reading.
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Got it.
Brajesh Kumar Thanks a lot! Thanks again for your time.
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Are there any codes available for kirchhoff migration based on wave equation solution? What I have got so far is the one from CREWES MATLAB library which has simple geometrical migration with amplitude and phase corrections. But It is not based on wave equation.
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Dear Lavnish Gupta, indeed it was a long time ago the time that Fortran and C were used mostly for those purposes.
I did not used Madagascar suite, but you can check under system/seismic
Best Regards.
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I am doing a wound healing assay and I have data for 5 concentrations at 0hr,24hr,48hr,72hr. What statistical analysis should I do and what will I infer from that analysis?
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I think you can further clarify the question.
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I am looking for a good topic on migration for working on a proposal for PhD. It would be better to have something on latest migration issues being spoken about or anything interesting. Something could be related to the UK.
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I think it would be good to explore health care management for migrants in countries with different health insurance models
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I need the migration depth of Nitrofurantoin in soil, to formulate exposure scenarios for crops. I haven't been able to find any research that has done this, as it appears not a lot is known about this antibiotic. I was wondering if there was a way I could reasonably estimate this?
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If there is a local company that extracts and analyses organic contaminants in soil, you could ask them to recommend an extraction method. The extraction is probably more important than the analytical method, which may be quite standard by GC or LC (don't know the molecule in question). The longer the molecule has been in soil the more likely it will be a "bound residue", and harder to extract. Using a standard extraction protocol approved by the environment agency in your country would add regulatory legitimacy to your work.
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I found a very interesting paper published on Academia by Premendra Priyadarshi https://www.academia.edu/34474584/Of_Mice_and_Men_DNA_Archaeological_and_Linguistic_correlation_of_the_two_linked_journeys_of_mice_and_men?email_work_card=view-paper and would be interested by your comments, as it shows that rats, mice and men migrated together between 20.000-10.000 years ago in link with the expansion of agriculture from India by two routes, a Northern one by Central Asia and Russia and a Southern one by Iran and Anatolia to France and tends to confirm my theory expressed in my papers and that Indo-Europeans came from North Western India and Central Asia. Best regards, Xavier Rouard
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Thanks for your support, best regards, Xavier
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Good day everyone. I'm doing Diploma Research on E-residency and digital identities (international relations) and I found myself in a bit of trouble about answering the question of what does Estonia achieved from the policy so far, what are the political risks, and what are the benefits?
To make a little intro, I want to concentrate on the soft and hard powers states can gain by selling their governance services and legislature to the foreigners and therefore increase the population (in a digital way). Also, I'm mentioning: 1. diaspora (which Estonia can connect back to their homeland), 2. complex citizenship cases (like in Estonia itself (ethnic Russian minorities), and like in northern Ireland (demand on double citizenship), ..), 3. The possible change of traditional understanding of nation-state (borders, citizens...), 4. Blockchains and involvement of private companies in providing government services, 5. collecting private data about e-residents. Can you help me out and like to give me directions?
Thank you in advance.
Best regards,
Luke
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Thank you Douglas Raybeck, it does makes sense. Shouldn't have missed that.
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We are looking to understand migrational movements of commercial fish species but we want to understand not only the adults (1.5m) but also the 0.5m. How technology is moving on this topic?
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Thank you both.
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i did the transwell migration assay and wound healing assay to reserch the cell migrtion ability. but the result is different. so I want to ask, what the different between these two assay? why is the ability to migrate diffenent?
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would you mind telling me which kind of difference occured between your two experiments? because I may meet the same question...thanks!
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Which cells between MCF-7, MDA-MB 231, MDA-MB 453 and the non-cancerous MCF-10A have the fastest migration rate ? And other breast cancer cell lines ?
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I can not confirm on others but...I can comment on MCF7.
Initially on passaging the cells are very slow growing....but once when 1-2 passages are done and the cells are confluent, they grow steadily....additionally they tend to grow one over the other...not in a monolayer....due to which they are not most preferred cells for migration assay (based on personal experience).
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I follow some Eurasian Spoonbills (Platalea leucorodia) with GPS-telemetry in Hungary.
They stay within the Carpathian Basin during their dispersal in summer/autumn. Dispersal movements occur in all directions, including in the southward direction as well in summer and autumn in the Carpathian Basin. Of course, Spoonbills before their autumn migration move in the southward direction, and birds may stop for hours/days/weeks in proper wetlands of the Carpathian Basin. As soon as they leave the Carpathian Basin they are clearly on their autumn migration.
Generally, "my" Spoonbills that were hatched in Hungary can breed any suitable wetland in the Carpathian Basin. Adults visiting even 3-7 colonies as soon as they reach Carpathian Basin in spring when they move northward direction.
Could you recommend publications, or share your experience how can I separate post-fledging/post-breeding dispersal from autumn migration? And, how can I separate pre-breeding dispersal of adults from the end of spring migration?
Thank you for your answer and your kind help in advance!
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I think:
1. Calculate directional level of movement
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Container technologies such as Docker, or Kubernetes are widely used in the industry for virtualization deployments. Edge computing paradigms such as MEC and Fog are intending to leverage such infrastructure for their feasible deployments. My intention is to simulate and emulate the service migration process of such an edge computing scenario (migration from one edge computing node to another) to understand the security aspects of them. Thus, I have the two questions;
- What is the best simulator I can use to implement a migration model (preferably MDP)?
- In order to emulate, should I use existing tools like Openshift, Kubernetes...etc. ; Do they have the migration function? or do I have to implement such a platform from the scratch using docker engine?
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Thank you for sharing this question
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1 how we can migrate switch from one controller to another controller in software defined networks?
2 methods or algorithms used for this switch migration in order to balance the load between controller and it simulation tools?
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I will assume you work on an OpenFlow-based network.
An OpenFlow switch can connect to multiple controllers and a controller can be in one of three possible states regarding the control of a switch: equal, master, and slave. A switch can have multiple controllers in an equal state, but only one controller in the master state (the other controllers will automatically fallback to the slave state).
You can start your research by searching for SDN controller load balancing and partitioning on Google Scholar and Scopus.
Mininet and docker will become your best friends. With these tools, you can build complex topologies without the need for expensive hardware equipment or real testbeds.
Hope I helped!
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Odonata experts, nice to meet you.
My name is Jose Alejandro Cuellar, an odonate researcher from Colombia. I am sending you this email because I am doing a review article on Odonata migrations in Latin America. For this, I ask you in the kindest way to help me with his information. For this, I send you a few questions that you could answer in case you have sighted, documented, published, or registered any case of dragonfly breeding in Latin America or Spain.
Please answer the questions for each case of migration you have observed:
Question 1: What species or species have you seen carrying out migration processes?
Question 2: During what time of the year did you record the case of dragonfly migration?
Question 3: Where (Country, town) did you see the case of dragonfly migration?
Question 4: Towards what direction or locality considers that the odonates that were migrating are headed?
Question 5: You have seen this same process in a repetitive or cyclical way over time?
Thanks for your help, this information will help me too much with the preparation of the review document.
Sincerely,
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kindly see
Seasonal Migrations of Pantala flavescens (Odonata: Libellulidae) in Middle Asia and Understanding of the Migration Model in the Afro-Asian Region Using Stable Isotopes of Hydrogen Sergey N. Borisov 1,*, Ivan K. Iakovlev 1 , Alexey S. Borisov 1 , Mikhail Yu. Ganin 2 and Alexei V. Tiunov 3 1 Institute of Systematics and
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I have been using an 8um pore membrane and the tests are bad.
Could it be due to the pore size?
I am not able to see differences between the stimulated condition (with MCP1) and the condition without the chemotactic agent. It seems that all the cells are passing through the membrane. My protocol: I add the macrophages in the inserts (50,000 cells) with pore size 8um. After 4 hours of incubation, the cells are fixed to the inserts with 4% PFA, washed and stained with violet crystal. Can someone help me? Thank you.
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the pore size of transwell for monocyte is less than 8um and it is maybe proper to be 5 um
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Good morning everebody.
We know that human migration is a phenomenon that affects the past, present and future of humanity. Today, migration has become globalized. Its influence can be seen in the diaspora of migrants, in the countries and societies where migrants come and receive them. How do you assess the impact of the migration process on the ethical environment? Is migration improving the moral environment or is it negatively affecting the morality?
Thank you in advance for your feedback and attitude!
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Demographic balance is still necessary, despite technological advances and production achievements. Robots or intellectual property cannot "fill" pension funds or social security funds; they need people, workers, productive workers. The issue of internal security of some countries today connects with migrants, with their uncontrolled influx and possible impact on the security, economic and political situation in the country, and the attack on national identities. Habermas points out that moral orientations of consciousness can be emotionally colored in different ways, including disgust or fear, grief or love. If similar behavior is "completely objective" concerning any man, you can "clash" with him, but not to quarrel."
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Hi there,
I am working with Treemix using thousands of SNPs to visualize the relationships between populations of my species of interest.
The manual is quite simple and although I can technically create the trees with different numbers of migration events, how do I decide which one is the right one?
I've read something about looking at the log likelihood values but I don't know how to do this (in R?).
Any help would be much appreciated.
Thanks!
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OptM , an excellent software
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I have used the BrainViewer for MATLAB, but since i have migrated to python, I do not know any package that helps for a nice publication-quality images of connectivity.
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There are a lot of nice tools and projects:
The human Brain project https://www.humanbrainproject.eu/en/ and the connectome project http://www.humanconnectomeproject.org/
nilearn: Machine learning for NeuroImaging
freesurfer
pycortex
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Hello, dear researchers,
What kind of reliable sources do you suggest to obtain individual-level migration data?
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I'd recommend running qualitative research and use Narrative Analysis (See:
Chapter Narrative
)
Also, here's a list of our recent publications that might offer you some directions concerning your research.
Other secondary data could be obtained from humanitarian organisations like UNHCR (See https://www.unhcr.org/uk/).
I hope this helps.
Many thanks,
Ali
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It has been obsessed since long that millions of birds were migrated every year between the countries and on contenant level. Migration was happened due to seasonal variation, environmental conditions, availability of feed and some other factors. Kindly suggest the impact of birds migration in the spread and outbreaks of different diseases.
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I would like to assess how the reasons of migration varies across type of environmental migrants, and therefore, how it could be useful for modelling adaptation strategies for the future migrants.
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Hi Chari, I would suggest you start with a pre-existing model changing some of its parameters. Are you using netlogo? If yes and if you are a starter, "An Introduction to agent-based modeling" by Uri Wilensky and William Rand is a nice book to start. I also found a master thesis on migration agends: https://scholarworks.sjsu.edu/cgi/viewcontent.cgi?article=1621&context=etd_projects
An other option is Gama. Again, you may find many ready-to-use models there.
Ioanna
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I trying to calculate the migration rate between genetic clusters obtain with STRUCTURE software with AFLP markers.
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Ur question is not clear enough to give a reply.
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I want to estimate the impact of internal migration on rural economy in Bangladesh. What will be the dependent variable (s) in this context? is it only be the flow of remittance into the rural area from internal migration?
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Hi, I want to find the impact of internal migration and agricultural productivity on rural development of Pakistan. What will be the best dependent variable? Can I take urbanization as one of the dependent variables? and what independent variables can I pick?
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How can we explain with neo-functionalism the migration challenges that the European Union faces since 2015 and the impact of the migration challenges
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Dear Reema Al-Tawil,
There was recently a special issue in the Journal of European Public Policy which examined how European integration theories can be applied in the current context and to better understand current crises. One of the articles is linked below, perhaps this or the other articles in the special issue are helpful.
All the best, Florian
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Equation of state (EOS) modelling in CONVENTIONAL reservoirs is well established and has been successfully employed in PVT studies of basins globally for many years. However, what is the current status of EOS modelling in UNCONVENTIONAL reservoirs, especially those that have undergone significant uplift and late secondary migration of high-maturity gas? (e.g., Montney Formation of western Canada; Woodford/Meramec formations, Arkoma and Anadarko basins)
Such unconventional reservoirs respond dynamically to naturally changing PVT conditions and may end up with intimately co-existing fluids that are not in equilibrium: for example, separated and migrating gas may exist in large “unconfined” pores whereas supercritical fluids exist in small “confined” pores. Such fluids can present problems for PVT recombination and the results may not reflect the in-situ condition of fluid segregation at the micron scale.
I wonder what expert opinions have to say about the above and the the current status of EOS modelling in unconventional reservoirs. I welcome any responses that can shed light on this.
Thanks, Jim.
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I agree with you. Equation of state (EOS) modelling in conventional reservoirs is predetermined and does not require special study and has been routinely performed in oil and gas reservoirs for many years ago. From an engineering and technical point of view, Equation of state modelling in unconventional reservoirs is very important. However, Conventional reservoirs, after the second half of production life, are in a similar situation to unconventional reservoirs, where Equation of state modelling will be important to them. By drilling several wells (infill wells) in a pre-developed field, the equation modeling results in these reservoirs can be improved.
Thanks, Kio
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VLDLr play important role in cholesterol uptake, metabolism of lipoproteins and neuronal migration
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One does not need to know TG levels to predict the population at risk of atherothrombotic disease (ATD). TG are not much present--if at all--in ATD plaque. Once one accounts for HDL-c levels, TG wash out as an ATD risk factor. For the prediction of the population at risk of ATD, see my publication in EC Cardiology a few months ago.
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I will use 6 well plates. I'm studying the migration of the macrophages? Is it possible cell be migrate after it cell already differentiate monocytes into macrophages?
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A quick intuitive response to the question on whether PMA-differentiated THP-1 can migrate: No, as far as I know from imaging experiments. The cells become very large, spread out and don't look very active or motile. The differentiation primarily turns them to the gene expression profile needed to study macrophages, but that does not necessarily reconstitute the phenotype in vivo.
Questions about how to culture and differentiate THP-1 have been asked many times in this RG forum.
Briefly, the optimized dose is 5 ng/mL PMA. The exactly procedure can be found in this highly cited paper:
Good luck!
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I am looking for quantitative data and analysis of the domestic migration of teachers. More specifically, I want to know more about the effect of the domestic migration of teachers on their teaching quality and on their students' academic performance I would like to receive recommended articles on the topic.
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I assume that the migration of a teacher is due to a school manager that sought him/her for his qualification. Therefore, I expect that his/her students perform better at school.
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