Questions related to Microvascular Surgery
Microsurgery is a technique which provide a wide range of possibilities in different surgical specialities. But what your indications for microsurgery in your field? Do you think that having good microsurgical skills could improve your surgical technique?
Conference Paper Habilidad en Técnicas de Microcirugía
I am focusing on the neo-microvascularization originating from pancreatic adenocarcinoma. The pancreatic vasculature is of great physiological/pathological significance. Does anyone have a protocol for microvascular endothelial cells isolation from human pancreatic tumors? I'm deeply grateful to your help!
I am currently working on my thesis and I am looking for more detailed articles about the surgical procedures used in hands and fingers re-plantations.
Could someone share, in your own opinion, the simpliest method to isolate and characterize mouse brain microvascular endothelial cells?
I'm going to start culturing these cerebral endothelial cells:
Eventually I want to perform tube formation and migration assays with them. In literature I could not find tube formation assays with these cells, as compared to HUVECs where you find the most info. I wonder if you have observed tube formation capacity. The company could not tell.
I also need to understand better what coating would be best, and what growth conditions. I need to find the "minimum" good conditions, preferably in defined medium without serum, as I will try treatments to see enhanced proliferation and angiogenic capacity, but this might be modest.
Please reply here if you have experience with these cells or with a particular aspect of it. If you don't have them from Cell Systems, but isolate them yourself, please reply anyways, your experience would be very helpful and appreciated.