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Microvascular Surgery - Science topic

This forum is for discussing difficult cases and problems in microvascular surgery.
Questions related to Microvascular Surgery
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Microsurgery is a technique which provide a wide range of possibilities in different surgical specialities. But what your indications for microsurgery in your field? Do you think that having good microsurgical skills could improve your surgical technique?
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Hi,
I agree with everyone here and being teaching microsurgery for many many years to many different surgical specialties and to surgeons and not only surgeons with different experiences proved to me that skills like micro significantly improve quality of technical skills everyone who has it. It not easy to obtain but in a way of learning microsurgery people also learn quite many other skills as well ( sometime without even realizing it :) like gentleness of handling and dissecting, attention to details, self-control and patience and decision making, etc I really believe that learning and using microsurgery is a very useful in many aspects and should be taught to all surgeons while they are in training even thought they are not will be using it in practice, they just will be better surgeons !!! thank you for the opening this discussion. 
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I am focusing on the neo-microvascularization originating from pancreatic adenocarcinoma. The pancreatic vasculature is of great physiological/pathological significance. Does anyone have a protocol for microvascular endothelial cells isolation from human pancreatic tumors? I'm deeply grateful to your help!
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We have used MACS system using CD31 antibody after making monocellular suspension from PDAC.
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I am currently working on my thesis and I am looking for more detailed articles about the surgical procedures used in hands and fingers re-plantations.
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Unfortunately the couplers are for veins only, ment to be used for reducing surgical time in larger vessels anastomoses and require an excess length of vessel which is not really available in most replants. There have been several research projects in the 80's trying to address the question you asked such as using lasers instead of sutures, doing continuous as opposed to interrupted sutures, sleeve anastomosis etc. I suggest you look up these studies
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Could someone share, in your own opinion, the simpliest method to isolate and characterize mouse brain microvascular endothelial cells?
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The culture medium was Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 1.5 mg/mL sodium bicarbonate, 15 ug/mL bovine endothelial cell growth factor (bECGF), antibiotics, and 10% fetal bovine serum (FBS).
However, this is very old protocol. I think that there are several endothelial specific medium (commercially available)
And you can use MACS or cell sorting combined with this method.
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I'm going to start culturing these cerebral endothelial cells:
Eventually I want to perform tube formation and migration assays with them. In literature I could not find tube formation assays with these cells, as compared to HUVECs where you find the most info. I wonder if you have observed tube formation capacity. The company could not tell.
I also need to understand better what coating would be best, and what growth conditions. I need to find the "minimum" good conditions, preferably in defined medium without serum, as I will try treatments to see enhanced proliferation and angiogenic capacity, but this might be modest.
Please reply here if you have experience with these cells or with a particular aspect of it. If you don't have them from Cell Systems, but isolate them yourself, please reply anyways, your experience would be very helpful and appreciated.
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Dear Virginia,
I have used the Matrigel (BD)  and EGM media for tube-formation assays of hBMECS.
My paper may help for your study (2009, Hayashi S et.al, PMID: 19815818 )
Best to you!