Microbiology - Science topic

Tuberculosis, as a contagious pulmonary disorder, may spread easily from an infected person to a non-infected person. The Microbiologically Confirmed Pulmonary TB Survey done from 2019-2021 in a population aged ≥ 15 years shows Delhi with 534 per lakh at the top among the high prevalence 20 states of India. The gravity of incidences and the increas...

The emergence of Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI TOF MS) technology has expanded the capabilities for identifying microorganisms in clinical labs, replacing traditional biochemical testing with a proteomic approach. In the present study, we compared results between the two commercial MALDI TOF MS...

Antibiotics that possess the capability to inhibit or eradicate bacteria have been widely employed in the fields of agriculture and veterinary medicine over the years. However, the excessive use of antibiotics has led to significant challenges regarding antibiotic pollution in food. Given the established risks of antibiotic contamination and the ne...

Low and persistent levels of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA/protein/virus can be detected in clinical samples months after infection, possibly related to the emergence of SARS-CoV-2 variants or development of long coronavirus disease. Here, we present a protocol to detect low levels of viral RNA together with prote...

Eradication of HIV-1 latently infected cells is an important issue in HIV treatment. However, there are limited models available to assess therapeutic efficacy in vitro. Here, we present a protocol for establishing a variety of HIV-infected Jurkat cells, including productive and latent status, evaluating the efficacy of antiviral agents, followed b...

There is an increasing demand to guarantee the safety of the food supply due to the public's growing concerns about food safety and foodborne illnesses. It has been demonstrated that gamma irradiation is a potential technique for safely eliminating a variety of common pathogens. The extra advantage of gamma irradiation technology is that it prolong...

Background: Infective endocarditis (IE) is defined as the infectious and inflammatory process of the heart’s internal structures. It can be caused by a broad group of bacteria and, rarely, fungi, with potentially life-threatening consequences. Objective: To profile bacterial resistance and identify mortality risk factors in IE patients. Methods: Th...

Commercial harvesting of American ginseng (Panax quinquefolius) results in root debris in the soil, but the rate of decay is unknown. In this study, post-harvest root debris decayed mostly over the fall and winter, with almost no ginseng debris remaining in the soil by late spring of the following year. However, a small number of intact pencil-shap...

The chronic wounds (CW) bed preparation requires an integrated approach using wound assessment, microbiological and histological analysis, which will avoid skin graft failure. The aim was to evaluate the possibility of using microbiological and morphological parameters of CW assessment to substantiate the choice of tactics of wound bed preparation...

Physical properties like shape, volume and size affect the dynamics of biological systems. Along these lines, we focus on the topological properties of biological fluids and their biochemical and physiological outcomes. We take as a paradigmatic example the salivary fluid and describe how its topological features may affect the physiopathology of t...

Physical properties like shape, volume and size affect the dynamics of biological systems. Along these lines, we focus on the topological properties of biological fluids and their biochemical and physiological outcomes. We take as a paradigmatic example the salivary fluid and describe how its topological features may affect the physiopathology of t...

This study aims to find the current various processes for the almond producers and the extractors of shea butter in Burkina Faso. The survey was conducted in seven provinces. Three approaches to data gathering were used: group interviews in which forms were filled out during interviews; individual interviews in which the interviewer asked one perso...

Biopesticides address issues related to pest resistance and environmental impact while ensuring human safety. Despite the challenges of meeting industry expectations, this eco-friendly solution is facing difficulties gaining traction in the pesticide market. Farmer adoption of biopesticides, particularly cost-effective microbiological options, lags...

Objective: to analyze the physical and biological barrier characteristics of cotton fields used as a sterile barrier system after multiple use and processing cycles in the clinical practice. Method: an observational and longitudinal study to monitor and evaluate 100% cotton fabric used as a sterile barrier system in a medium-sized hospital. Samples...

Soil harbors a huge diversity of microorganisms and serves as the ecological and social foundation of human civilization. Hence, soil health management is of utmost and consistent importance, aligning with the United Nations Sustainable Development Goals. One of the most hazardous contaminants in soil matrix is potentially toxic elements (PTEs), wh...

The purpose of this study was to evaluate the effect of temperature (2 °C and 6 °C) and storage duration on the quality and attributes of hens’ breast meat after their laying periods. The study included physicochemical characteristics (pH, drip loss, colour, shear force), microbiological quality (total Enterobacteriaceae family and Pseudomonas coun...

Objective: This work aimed to evaluate the microbiological safety of bioinputs produced in Mexico. The main reason for this evaluation is that bioinputs are products made from the region's manures, plant residues and raw materials. The transformation of these raw materials is carried out by microorganisms present. The process goes through three sta...

Zusammenfassung Die Abklärung chronischer Wunden ist essenziell für die Einleitung einer kausaltherapeutischen Behandlung. Zur diagnostischen Einordnung der Wundgenese kann es erforderlich sein, eine Biopsie zur histologischen und/oder mikrobiologischen Aufarbeitung zu entnehmen. Besteht klinisch Verdacht auf eine spezifische Ursache der Wunde wie...

Objetivou-se avaliar a qualidade microbiológica e o pH da carne bovina moída comercializada em supermercados no Sul da Bahia, Brasil. Foram realizados coleta e preparo de amostras de “carne moída” nas quais o pH foi aferido e as análises microbiológicas foram realizadas com a contagem de bactérias aeróbias mesófilas, bolores e leveduras, determinaç...

Candida spp. is one of the most important components of human micro ecology. Most of the patients who diagnosed with candiduria do not have any apparent symptoms and it is certainly very difficult to differentiate between bacteriuria and candiduria in symptomatic patients. The main aim of this study to determine the frequency of candidiuria in pati...

With the increased growth in the cosmetic industries, it has become quite common to seek the services of professional beauticians thereby increasing the tendency of sharing makeup applicators. This study investigates the microbiological contamination of makeup applicators (Brushes and sponges) used by beauticians and to determine the level of hygie...

Game-based learning environments (GBLEs) incorporate game mechanics, i.e., learning and assessment mechanics, to increase domain knowledge while maintaining learner engagement. Although GBLEs have been developed to improve science learning, learners have attained lower science achievement scores over the past decade as they progress through school....

This study extends the reports of PAP in SA, in this case a 29-year- old HIV-negative black African woman with no history of smoking. Patients with respiratory symptoms who are microbiologically negative for TB should be carefully evaluated for an alternative diagnosis.

Filtration backwashing is necessary for the effective operation of membrane modules, and intermittent aeration helps to remove nutrients, which can save energy and effectively control the occurrence of membrane contamination. In this study, membrane contamination was controlled using an MBR in intermittent aeration operation mode and a filtration b...

Small-scale cereal-based food production companies have recently been growing in Ouagadougou. The promoters of these companies do not always apply good hygiene practices (GHP) during their activities, which can lead to the production of food of unsatisfactory quality, which can threaten the health of consumers. In view of this observation, this stu...

From june to september 2004 a water research was carried out in six stations that are located at the following microbasins: La Laucha (S1), La Lejía (S2, S3 and S6), and La Rastrojera (S4 and S5), those microbasins are localized at the Durania municipality in Norte de Santander department. This study was carried out in order to evaluate water quali...

In this study, it was aimed to isolate aerobic bacteria, yeast and fungal microorganisms from milk samples taken from clinically healthy dairy cattle of different breeds. In the study, a total of 328 milk samples were taken from 4 udder quarters of 82 cows. Different microorganisms were isolated from 257 milk (78.35%) samples. A total of 265 microo...

A microbiologia é o ramo da ciência que historicamente sofre com desafios no seu ensino. Este trabalho objetivou avaliar a qualidade do conteúdo de microbiologia abordado no livro didático do ensino médio, adotado pela Secretaria de Educação de Mato Grosso, para o ensino médio das escolas estaduais dos 141 municípios do estado. Trata-se de uma pesq...

Background Despite the importance of multidrug-resistant organisms (MDRO) bacteremia, especially extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-E) or carbapenem-resistant Enterobacteriaceae (CRE), the disease burden of those has not been investigated in detail. Thus, we tried to evaluate the clinical outcomes and estimate the soci...

Background Delayed treatment of bloodstream infections is associated with increased morbidity and mortality. Conventional methods for organism identification and susceptibility data from blood cultures can take about 2-5 days. Technological advancements in gene-based polymerase chain reaction tests amplify DNA targets from positive blood cultures a...

Helicobacter pylori infection is one of the most common chronic bacterial infections worldwide. The infection is usually acquired during the first years of childhood and transmission from infected mother is considered an important epidemiological factor. The aim of this study was to assess the H. pylori positivity in Bulgarian symptomatic children...

Background Methicillin resistant Staphylococcus aureus (MRSA) bacteremia is a clinically relevant syndrome with high mortality. Combination therapy with beta-lactam antibiotics has been employed in persistent MRSA bacteremia with reported clinical success. Ceftaroline is a beta-lactam antibiotic with activity against MRSA, which has been commonly u...

Background Cefiderocol (CFDC) has broad activity against Gram-negative (GN) pathogens. This study describes the CFDC usage in treating non-COVID patients with microbiology data in US hospitals from Jan 2020-Jun 2022. Methods This retrospective multicenter observational study included adult non-COVID hospitalized patients treated with CFDC consecut...

Background Community-acquired pneumonia (CAP) is a common cause of hospital admission, with an annual incidence of 24.8 cases per 10,000 adults in the United States. To establish a microbiological diagnosis, most guidelines recommend using urinary antigen tests (UATs) in addition to respiratory samples in severe cases. However, it remains unclear h...

Suncus murinus is gaining prominence as a laboratory animal; however, there is no generally accepted method for microbiological monitoring. This study aimed to apply non-serological microbiological monitoring of laboratory mice for S. murinus and identify the subdominant species obtained by culture methods for microbial assessment. Culture and PCR...

Background Patients with chronic kidney disease (CKD) have high risk of developing TB and numerous studies have been done on presentation and outcome of both conditions. However there is a lack of data on regional burden of TB in this group of population. We set out to study on clinical presentation and treatment outcome of TB in patients with mode...

Background Carbapenem-resistant Gram-negative infections remain a serious threat due to their increasing incidence and high mortality rates. Since 2015, various antimicrobials with in vitro activity against carbapenem-resistant Enterobacterales (CRE) have been approved for use. There is limited evidence on the safety and efficacy of these novel ant...

Background Elizabethkingia species are multidrug-resistant emerging pathogens that have caused outbreaks in healthcare settings and are associated with high mortality rates (20-40%) among infected patients. We experienced an increased number of E. anophelis isolates on our high-risk pulmonary medicine unit (PMU) in October 2022. An outbreak investi...

Background Updated cascade reporting of antimicrobial susceptibility (CRAS) for high-risk AmpC-producing Gram-negative organisms was implemented by the antimicrobial stewardship (ASP) and microbiology teams in June 2022 based on recent IDSA guidance. The CRAS focuses on highlighting weak inducers and poor substrate antibiotics such as cefepime and...

Background Blood cultures help to detect causative agents in bacteremia and are important for targeted therapy. Adequate blood culture volume is considered as 8-10ml in each bottle and at least 2 aerobic and 2 anaerobic blood culture bottles are recommended. Inadequate blood culture fills are common and may affect yield. Figure 1 Methods The study...

Background Hospital-acquired bacterial pneumonia (HABP) and ventilator-associated bacterial pneumonia (VABP) are associated with high mortality rates. In these patients, there is an unmet need for effective antimicrobial therapy. This study enrolled participants mainly from China to support the regional use of imipenem/cilastatin/relebactam (IMI/RE...

Background Infective endocarditis (IE) results in high morbidity and mortality. Guidelines for IE recommend prolonged intravenous antibiotics that can result in extended hospitalization, but some patients experience patient-directed discharges (PDD) prior to completion. There is a current lack of literature clearly defining long-term outcomes and o...

Early blight disease caused by Alternaria species is the most destructive fungal disease of tomato in Ethiopia and in southern Tigray in particular. However, the importance and distribution status of the disease has not been studied in this area. Besides, the reaction of released tomato varieties to the disease was not well documented in Ethiopia....

A severe inflammatory response that eventually destroys the underlying tissue characterizes periodontal diseases, which have a mixed microbiological origin. Several local or systemic risk factors can impact the appearance and progression of periodontal diseases. Although it can affect infants and teenagers as well, periodontal disease is more commo...

Introduction In pre-operatively presumed aseptic nonunions, the definitive diagnosis of infection relies on intraoperative cultures. Our primary objective was to determine (1) the rate of surprise positive intraoperative cultures in presumed aseptic long-bone nonunion (surprise positive culture nonunion), and (2) the rate of surprise positive cultu...

Rapid microbiological reports to clinicians are related to improved clinical outcomes. We conducted a 3-year quasi-experimental design, specifically a pretest–posttest single group design in a university medical center, to evaluate the clinical impact of rapid microbiological identification information using MALDI-TOF MS on optimizing antibiotic pr...

Water is an important constituent of all life forms and essential for its survival. Satisfactory supply of clean, safe, and hygienic drinking water is imperative for health. The current study has been designed to assess the microbial quality of drinking water in and around Rathinamangalam village, Tamilnadu. It is a cross sectional study. A total o...

Background There is concerted effort to reduce the burden of 6 months antimicrobial treatment for tuberculosis (TB). Early treatment cessation at 8 weeks is effective for most but incurs increased risk of disease relapse. We tested the hypothesis that blood RNA signatures of TB disease or C-reactive protein (CRP) measurements discriminate microbiol...

Adequate and rapid microbiological diagnosis of sepsis is essential for correct treatment, having a direct impact on patient prognosis. Clinical Microbiology Services must adapt fast circuits that allow prioritizing and individualizing the diagnosis of these patients. The measures adopted should not be based solely on the incorporation of new techn...

Group A Streptococcus (GAS) can cause a broad array of clinical manifestations and complications. Recently, in post COVID-19 postpandemic months, there has been an increased incidence and severity of invasive infections in the pediatric age group in Spain and other European countries with high morbidity, affecting mostly to young children, associat...

A very important role in determining the quality of water is the assessment of its microbiological quality. Water quality control, which could pose a direct threat to human health and life, is performed in the case of water produced at water treatment plants, tap water, or water in swimming pools. However, these traditional methods used to assess i...

Although Holder pasteurization is the recommended method for processing breast milk, it does affect some of its nutritional and biological properties and is ineffective at inactivating spores. The aim of this study was to find and validate an alternative methodology for processing breast milk to increase its availability for newborn babies and redu...

Purpose: This research aimed to evaluate the physical-chemical and microbiological quality of water in a certain region of the Meia Ponte river basin, through monitoring water quality parameters, and also to verify the interference of the rainy season in water treatment. Methods: Physicochemical and microbiological analyzes were carried out, evalua...

Human Pseudomonas infections have high morbidity and mortality rates. Pseudomonas bacteria can cause sepsis or septic shock; they produce biofilm and commonly exhibit a multidrug-resistant phenotype. The choice of antimicrobial therapy in many cases is challenging, and deep knowledge of clinical, microbiological, and pharmacological issues is requi...

In clinical parlance, a new infection in the bone is referred to as acute osteomyelitis. This illness is more common in youngsters and spreads through the bloodstream rather than through direct contact. When it occurs in adulthood, osteomyelitis is typically a subacute or chronic infection that develops as a consequence of an open injury to the bon...

Objective The gold standard for hand hygiene (HH) while wearing gloves requires removing gloves, performing HH, and donning new gloves between WHO moments. The novel strategy of applying alcohol-based hand rub (ABHR) directly to gloved hands might be effective and efficient. Design A mixed-method, multicenter, 3-arm, randomized trial. Setting Adu...

Objective: To analyze the value of microbiological testing in the treatment of urinary tract infections. Methods:A total of 552 patients with urinary tract infections, diagnosed and treated between January and December 2021, were selected for this study. They were divided into observation and control groups, each consisting of 276 cases, using the...

O ensino da microbiologia tem se tornado cada vez mais relevante, considerando os recentes acontecimentos da sociedade. No entanto, ainda é abordado de forma simplificada. Assim, o objetivo do estudo é entender como o tema está sendo desenvolvido. Para isso, foi desenvolvido um questionário online, e a coleta de dados foi realizada por meio da técn...

SARS-CoV-2 spike protein plays a key role in mediating viral entry and inducing host immune responses. It can adopt either an open or closed conformation based on the position of its receptor-binding domain (RBD). It is yet unclear what cause these conformational changes or how they influence the spike's functions. Here we show that Lys417 in the R...

The consumption of horsemeat is a traditional practice in several Asian countries, including Kyrgyzstan. This study aimed to evaluate chuchuk samples made form horse meat regarding their physicochemical and microbiological quality properties. For that purpose, 32 samples of chuchuk samples from bazars in the Chuy region of Kyrgyzstan were collected...

The identification of bacterial colonies is deemed to be crucial in microbiology as it helps in identifying specific categories of bacteria. The careful examination of colony morphology plays a crucial role in microbiology laboratories for the identification of microorganisms. Quantifying bacterial colonies on culture plates is a necessary task in...

Background Cough and sputum are major symptoms in cystic fibrosis (CF) that contribute to the impairment of quality of life. Methods This prospective single centre cross-sectional pilot study aimed to evaluate the results of a self-administered questionnaire assessing cough and sputum symptoms (2 domains), and their impact (2 domains) on daily act...

Osteomyelitis is an uncommon cause of a soft tissue mass and is diagnosed clinically by a blend of radiology, histology, and microbiology. This case report outlines a case of melioidotic osteomyelitis presenting as a suspected sarcoma. The diagnosis of melioidotic osteomyelitis was done against the diagnosis of sarcoma only after radiological evalu...

Many bacteria have the ability to survive in challenging environments; however, they cannot grow on standard culture media, a phenomenon known as the Viable but Non-Culturable (VBNC) state. Bacteria commonly go into the VBNC state under nutrient-poor environments or under stressful conditions. This review explores the concept of the VBNC state, pro...

Bacillus strains are ubiquitous in the environment and are widely used in the microbiological industry as valuable enzyme sources, as well as in agriculture to stimulate plant growth. The Bacillus genus comprises several closely related groups of species. The rapid classification of these remains challenging using existing methods. Techniques based...

In this study, we have shown that the strain Planococcus okeanokoites-indigenous to highly saline soil of Ezzemoul (Ain M'lila)-is capable to protect wheat seedlings against salt stress. It would be interesting to know its effect on later development stages.. The First International Seminar of Microbiology: Functional Micro-ecology in the service o...

Uric acid is an important component of milk, which has antioxidant properties and protects it from rapid microbiological spoilage. The article studies the change in uric acid content during unwanted spoilage of pasteurized and unpasteurized milk using a developed voltammetric sensor based on a carbon veil. The sensor allows to estimate the content...

The influence of oil hydrocarbons on soils with short-term and long-term pollution is considered. The regularity of the change in the number of microbiological community depending on the duration of oil hydrocarbon pollution has been established. The dependence of catalase activity on the time of contamination in the soil is shown.

The process of anaerobic decomposition of waste organic matter is a complex and crucial aspect of waste management, with profound implications for both environmental and public health concerns. This intricate process involves the activity of diverse microbial communities that play a pivotal role in breaking down and transforming organic materials i...