Microbial Isolation - Science topic

I have 16sr DNA sequence for a bacteria which shows 86% similarity while doing BLAST search. Does this mean it represents a novel genus? In that case, is it mandatory to do a DNA hybridization test?

Methanotrophs use methane as their sole source of C and energy. They are single biological entity, which can help in the mitigation of most potent GHG in the atmosphere. 

Microbiologists, research scholars or experts working in this feild

Ruminococcus albus

I want to screen soil samples for azotobacter and azospirillium, so is there any plate or chemical assay to quickly screen samples, after that when I identify it as any one above them, then please suggest any molecular or other method to identify the strain of the same..

A similar  question has already been  asked by Anil Khatape, and I  had  read the answers.. 

I have enriched the archaea in liquide medium containing acetate as sole source of carbon, and have added antibiotics. However, when transferring on solid plate (with acetate), no grow is observed..  I am wondering whether someone has experience in isolating acetoclastic methanogen on solid agar culture. 

Thank you in advance

I want to isolate hyper thermophilic bacteria in general (not a specific bacterium) but i do not Know the most suitable medium; would u help me plz? 

thanks

Hi,

I would like to cultivate bacteria from fish skin in a range of different medias and isolate them afterwards. I am wondering if anyone have experience with cultivation of bacteria from skin using a medium that contains mucins? Or other medias that might be suitable for cultivating skin bacteria?

Please suggest some standard pocedurte for isolation of polyethylene degrading bacteria from soil and water sample. Standard media and polythene sample is varying in different articles besides the incubation time and enrichment process. 

How lonng the clinical isolates of S aureus can be preserved at 2-8 degre celcius?

Thanks. Please is 16S rRNA sequencing similar to 16S rDNA sequencing. Secondly can you suggest a reliable laboratory that can perform molecular characterisation of Microbial Isolates with fast turn around time to me. I need answers urgently please

I want to enumerate and isolate zinc mobilizing microbes from wheat rhizosphere. Any one may guide me the most appropriate media along with the zinc source please for the same?                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 

Is their any easy method by which I could separate microbial cells from saliva samples of human. It would be really helpfull if someone can share related protocol.

I am working on biological nitrogen fixation and I wanted to screen the microbes for nitrogen fixation attributes. Is there a method to find nitrogen fixation by microbial isolates apart from Gas chromatography method (ARA).

What is the real meaning of the iterative coryneform bacteria isolated from samples of the waning of otorrhea episodes of chronic or recurrent otitis externa ?

I isolated many phages from marine water and  took a microscopic image for this phages but now I have many phages. I think this may be new phage but I'm not sure whether these are new phages or not. Also I every time I take microscopic images for phages isolated from one plaque, I see more than one phage in my sample. 

I read many articles about purification of phages from marine water. I did all that was advised but I don't get good results. Thank you for advise about all of these issues.

I was gram staining some bacteria in a microbology practical and when I look into microscope, I see handful of unknown objects that doesn't look like bacteria at all (refer to the photo attached). 

My supervisor has no idea about what it would be, but suspecting that they are some sorts of brown microscopic fungi. It is ~ 8 microns in length and ~2 microns in width and it seems to have been splitting out transparent cells that seem to contain chlorophyll inside.

Thanks everyone!

I am currently isolating those lactobacillus from dali products and I am using tomato juice broth with this formula:

for 400ml

Triptone 4g 

yeast extract 4g

80ml of filtrated tomato juice

I been having problems to growth them, they growth but really slow, can someone help me to see if the broth I am using is missing something? Thank you!

I am looking for some methodology that decrease the time of microbiological analysis. Today we expend 48 hours to know if a sample is contaminated by bacteria and almost a week to know if a sample is contaminated by fungi. I am looking for something that could indicate this contamination in hours or, if possible, minutes.

Do you know some methodology or device that could make this happens?

Thank you so much.

Lilian

I attached gel image with all problems on it,please analyse and give me right suggestion to optimize it.

isolation criteria

suitable media

preservation during transportation

incubation temperatures

The php pheneplate system is a microplate based assay that allows fingerprinting the phenotype of microbial isolates (or microbial communities), basing on the cinetic of utilzation of a set of C substrates. Although it has been used for medical research, there is no publications that I know of in which this method is applied to the soil microbial community. I believe it could be used to obtain information on the functional diversity of soil microbial communities, similarly to the biolog method. In the past, my colleagues obtained encouraging results applying the assay on a very sandy loose soil. As of now, I am having troubles obtaining reproducible results, having tried 1% pirofosfate solutions as extractant on a clay soil, and dilutions up to 10^-4, with and witout filtering the soil suspension. I believe the problem is not related to microbes contaminations, as I operated in a sterile environment, and my control plate does not suggest the presence of contaminant microorganisms.  Has anybody ever been able to successfully perform a php pheneplate assay on a soil microbial community? What is the best extractant to use? How much should I dilute the suspension before inoculating it into the microplates?

isolation of environmental mycrobaterium TB

Trying to make up some different media to isolate soil actinomycetes, and also ocean actinomycetes.  I was going to go with actinomycetes isolation agar in addition to a few others, but was wondering how important the asparagine really is.

Sodium Caseinate----2g

Asparagine---0.1g

Sodium Propionate----4g

Then some trace metal salts, and sometimes addition of glycerol.  

Was also looking at Soya Flour Mannitol media, Triptic Soy Agar, and Starch Casein Agar, with perhaps addition of autoclaved ocean water when isolating from saline mud samples.  

I'm working on mastitis organisms, even the general causative microbes are also taking a longer time for culturing so I'm waiting for good suggestions.

I have isolated actinobacteria from soil, but until now I only get one actinobacteria. Most of microorganism grow there is bacteria. I used Nystatin, penicillin, and sometime use benomyl. Did I use a wrong supplement?

For Screening.

I have a very promising consortium, which is able to grow almost in pure oil and degrade it as well. I want to isolate cells for further analysis and sequencing, but there is a problem. When I am trying to centrifuge the medium, nothing is happening (no pellet is visible, only some scums).

Does anyone have any experience with such samples and methods of isolation?

I have learned that for the identification of microbial isolate (especially bacterium) up to species/subspecies level one have to analyse 16S rRNA/rDNA sequence, %G+C content of the bacterial genome, DNA-DNA hybridisation and FAME analysis (as reported in many papers published in IJSEM journal).

What is the role of % G+C content in identification and what it means above 50% G+C and below 50% G+C content? which is better (above 50% or below 50% G+C) to relate the evolutionary relationship between isolated bacterial cell with other already reported . {>50% G+C content means evolutionary relationship or not?}

I've been using bacillus subtilis to isolate thermostable alpha amylase.

I found a large presence of lactococcus garvieae in milk and cheese samples.

We were trying to isolate some thermophilic bacteria when the idea suddenly struck us to isolate Thermus aquaticus from hot springs. But before approaching hot spring sources we wanted to try isolating some thermophiles from the soil.

I'm planning to sample several environments looking for Staphylococci species specifically. Is there any protocol permitting to select them efficiently? For example, which medium is the most selective? What are the biochemical tests which are reliable and discriminatory, easy to perform? Even if the selection is not perfect, I am looking for reducing the number of strains to sequence (for identity confirmation).

We have been trying to identify some of our bacterial isolates using the Biolog Identification system. Two of the isolates, which vary in their physiological fingerprints to an extent, have been assigned the same genus and species by the Biolog. Any clues?

We are currently handling a good collection of lactic acid bacteria and yeast isolates associated with various stages of an indigenous fermentation process for production of fermented bamboo shoot. Most of them are fairly identified by ARDRA and rRNA gene sequencing (similarity range 97-99%). However, being an untapped ecological niche which is not explored deeply, we are expecting this niche might harbour new novel species.