Science topic
Microalgae - Science topic
A non-taxonomic term for unicellular microscopic algae which are found in both freshwater and marine environments. Some authors consider DIATOMS; CYANOBACTERIA; HAPTOPHYTA; and DINOFLAGELLATES as part of microalgae, even though they are not algae.
Questions related to Microalgae
Hello everyone,
I am going to prepare either an alginate or carrageenan hydrogels for immobilization of microalgae. The microalgae will be expected to be retrieved from the hydrogels after finishing cultivation. In that case, hydrogels need to be destabilized/dissolved. Can anyone suggest any chemicals or processes for this ? I found some information about chelating agent like sodium citrate or EDTA as chelating agent for Ca2+ ion in alginate matrix, but not sure any other agents out there are available, especially for K+ ion.
Hello friends. I want to extract rubisco from spirulina microalgae does anyone have a solution? Do you have a proper protocol?
For some reason, I had no choice but to culture my microalgae in the same LAF that was being used for fungal studies as well. Now for obvious reasons I have started getting fungal contamination in my microalgal plates. I have changed the LAF and even on subsequent re-streaking, I am unable to get rid of it. Any suggestions as to how to make the microalgae free from fungal contamination?
I found a published photo where author writes that this is diatom species Hyalosira delicatula. But I have some doubts about such conclusion.
Unfortunatedly I do not have any photographs and the description of Hyalosira delicaula. So, can anyone familiar with the genus Hyalosira confirm or refute this conclusion?
This algae invade my culture lately (Chlamydomonas). It will caused the culture to clump and eventually die. This contaminant was motile, with more than 4 flagella observed.
Hello...
Two months ago, I bought pyrocystis fusiformis culture for my research. Unfortunately, no bioluminescence was observed, and the culture seemed to be deficient from the start.
Now I'm trying to buy a Pyrocystis fusiformis culture again and found a lab called PyroFarms, but before I order, I wanted to check if anyone had any past experience with them. or alternatively, if you could name any other trustworthy sources where I could buy a P. fusiformis culture, I would be grateful.
Hello everyone. Hope this question find you well.
I was recently reading about fatty acids because my master thesis is going to be about them and the most of their perspectives, like a review. And a question arose since I read in a book that there are some trans-unsaturated fatty acids found in nature (microalgae, bacteria, plant, etc), I can assume why and their reasons to be, but my question is that if these trans-unsaturated fatty acids can turn in cis fatty acids because some enviromental condition, as a reduction in the environmental stress to which it may have been subjected the sample.
I was searching for some information that can answer my question, but nothing at the moment. I'll appreciate so much your help with this. Thank you!
I found this specie in estuarine waters of Ecuador
Good afternoon, I am working with the production of Chlorella sp. in industrial waste and I would like to know what parameters are the most optimal to evaluate in this industrial waste... If anyone has any information about it, I would appreciate your contribution.
Good day All
I am busy cleaning up the microalgae strain Haematococcus pluvialis isolated from my bird bath outside. I have been battling to get rid of the Paraphysoderma sedebokerense (fungal parasite) on this strain for a very long time. Is there perhaps someone in this community with more experience who can advise me on which specific antibiotics and / or fungicides would be most effective for cleaning H. pluvialis? Your help will be much appreciated.
This is Haochen Zhang, a MA design products student from Royal College of Art. And Iâm currently working on a project about removing nutrition of flashed dairy manure. After learning some relevant materials, I realized that microalgae is a very effective means of treating wastewater, and I want to design products which can take use of microalgae (Chlorella) to help local rural residents transform the water to new resource. But now I lack of detailed and professional guidance. So I sincerely hope that I can get an opportunity to learn from you, and get more advice about this project.
Here are some questions which have been confusing me so much:
1. Will sterilization influence the effect of chlorella growing? If I want to sterilize the flushed manure water (100% sterilization), how much do I need to heat it to what temperature? And how long should it last? Can a sterile environment be achieved by simple boiling? Will high-temperature heating produce toxic substances in manure?
2. Is it necessary to re-dilution to feed chlorella with flashed manure water? Is it sufficient as a source of nutrition for chlorella? Do I need to add more nutrients? To what extent can the nitrogen and phosphorus in the water body be absorbed by chlorella?
3. What indicators should be paid attention to in the environment of cultivating chlorella? For non-professionals, is it difficult to cultivate chlorella with the water? What is the most suitable container volume for cultivating it?
4. Can the cultivated chlorella be directly used for fertilization (spraying or watering on vegetables)? Does it have a positive effect or additional economic value for agricultural production?
I sincerely hope that I can harvest precious opinions and opportunities to learn from you. Your suggestions must be very valuable to me. I hope with your help, my project can become a good design that has a practical effect on society! If possible, I hope to have more contact with you, thank you very much! !
Despite the high quality of fish-based ingredients in aquafeeds, reported unsustainability caused by their use in aquaculture, have raised global concerns and effort for replacing them.
Among the potential candidates ( insect meal & oil vs plant meal & oil vs microalgae meal & oil : Spirulina , Chlorella, etc. ), which ones have no side effects or donât generate new environmental impacts ?
Microalgae considered a a noble biomass for production of high value products, food and feed, Pharmaceuticals as well as Biofuels.
How does the F/2 medium help the growth of the Dunallila salina microalgae?
why do we use this medium?
What is the main mechanism of wastewater treatment through microalgae in open ponds and closed cultivation? How does it work when there is no oxygen and carbon dioxide in closed cultivation?
I'd like your advice on Pyrocystis Fusiformis growth. I purchased a culture a month ago and began cultivating it right away. Things, on the other hand, do not appear to be moving forward. There has been no bioluminescence observed yet, and the algae's growth has been poor. The culture was maintained at a pH of 8.2 and a temperature of 20 degrees Celsius with a 12-12 dark/light cycle. Cell counting under a microscope was used to monitor the algae's progress, but because I don't have much experience in the field of microbiology I wasn't sure of my count. I've attached a photo of what I see under the microscope, and I'm not sure if it's algae cells or not.
I'm hoping you could give me some suggestions on how to improve the growth of algae and its bioluminescence.
Hey all !
I am looking for the best method to prepare microalgal slides for TEM characterization, especially as I am working with nanoparticles.
Thanks all in advance :)
I want to know the pro and cons of this production?
Good morning, I have a question and I hope someone can help me. I have as inoculum chlorella vulgaris, with photoperiod of 12/12 hours, shaker agitation up to a volume of 500 mL and agitation provided by fish tank pump for volumes of 1 L and up.
I have observed the formation of a foam on the top of my crops, which I associate to a bacteria producing it but I have not been able to control that, any recommendations?
Also in the upper part of my containers it is forming like a green slime where my microalgae are staying and although I try to homogenize them they do not unite. Could this be associated with
Good morning, someone could help me to decipher what is causing this in my solid media, I planted chlorella vulgaris in plate count medium and in nutrient medium and I observed that some of them became fuchsia with the passing of lso days, could it be some metabolite or some microorganism?
Can I extract carotenoid pigment from microalgae (C. sorokiniana and D. salina) without freeze-drying?
I already searched and read references about drying process that we can use heat-drying method but that wasn't recommended because could cause degradation of carotenoid content. So if I don't have a freeze-dryer, can still extract the pigments from wet biomass?
Hello,
For a project aiming to produce Spirulina production for human use, I am lokking for a pilot scale (around 10 or 20 thousand liter capacity) PBR supplier..
I will appriciate if you suggest a good company producing LED light integrated tubular PBR in Europe or Asia.
thanks alot for your help
I recently published an article "Shining a Light on Wastewater Treatment with Microalgae" doi.org/10.1007/s13369-021-06444-3 https//rdcu.be/cE0C2 and now I would like to prepare a much shorter version of the article for publication in Frontiers for Young Minds. There are no publication charges, and this is probably the best way of communicating with the next generation of scientists. Because the article is intended for younger students the journal limits the length of manuscripts to 1500 words, 3 figures, and 5 references. The scope of the article will be determined by the team that agrees to collaborate on this venture. It is not required that authors are well versed in wastewater treatment, microalgae, or renewable energy. Rather it is only required that you have an interest in these topics and are willing to collaborate.
Can you help?
John Kilbane
We are starting soon an in vivo trial with broilers fed diets supplemented with microalgae and insect full fat larave. We will collect blood samples and I'd like to have your suggestions about the best parameters to be analysed on blood to evaluate the oxidative and metabolic status
I m working on biosafety evaluation of GMO microalgae, can anyone guide me which is better and acceptable method (lyophilisation or simple heat drying) for drying as i am going to use it as feed for fish? Also after harvesting pellet of algae, at what temperature i can store before drying, 4 C or -20 C, and for how long without affecting cell integrity?
My name is Stanley please help me with the name of the microscope that I can use to view the microalgae structure and able to isolate a single strain.
Actually for my thesis bg11 medium sample in scientific shop are unavailable. So for that any alternative to bg11 medium
Photoautotrophic regime is known to be not as productive as photoheterotrophic regime, mainly due to the fact that in photoheterotrophy microalgae has both light and organic carbon availability. Is there a situation where the opposite happens? What could be the possible explanations? I appreciate your thoughts on this.
Hello! I am trying to extract chlorophylls from microalgae, and I would love to ask how can I separate chlorophyll a from chlorophyll b? I want to get each of them separately. Thank you in advance.
I am running a drying model and would like to have the water content of a chlorella vulgaris microalgae, before drying. Thanks. best.
Currently I and my group members was tasked on a project on using carbon dioxide as main feed for microalgae product formation. So, we decided on using Chlorella vulgaris but now we are all stuck on balancing the equation since
1. we failed to properly formulate chlorella vulgaris
2. the pathway we have chosen does not show its by products
Without those 2 we cant do economic potential analysis to see whether its profitable for production. any ideas how we should proceed? we may need to change the microbes we use thus the product and that put us back into square one. btw we are trying to get beta carotene as our main product. and the pathway is chosen from. or have we gone the wrong steps?
I am making kosaric media using artificial seawater in various salinity for microalgae cultivation. At first, I tried to filter the seawater and then add chemicals. After autoclaving, there was some deposits at the bottom of the media. I also tried to autoclave the artificial seawater and media separately, and then let both solutions cooled down and added together in laminar flow. However, once I added the kosaric media into seawater, precipitation was observed and large amount of white crystalline substance at the bottom. Is there any other way to make it?
I want to quantify total carbohydrates in Hemerick media ( a media for microalgae growth). After I added phenol & sulfuric acid the sample becomes totally black. This problem isn't observed with artificial sea water media and the same phenol/sulfuric acid solutions.
Hi everyone. I am measuring carbohydrate content of some microalgae. While I was making the standard curve, I noticed that the cultural media (F2 medium and ESAW medium) can also react to phenol and sulfuric acid, leading to a strong yellow color which affects the final result. Do you have any idea why this happens? And how can I solve the problem? Thanks a lot!
I know that this depends on the rheological factors of the medium, but if you know any, please let me know.
Best Regards,
Roberto Novais
I am conducting an experiment to obtain enhanced antioxidants production in microalgae by implementing a 3x3 full factorials (three factors each at three levels) abiotic stressors. I would like to analyse my data using minitab software or jmp and obtain optimal levels of factor for maximum antioxidant production.
I am a PhD student, working with microalgae,
i have a doubt, can microalgae utilise water soluble cellulose like CMC as carbon source?
We are an certification body for organic farming. We also certify some producers of microalgae, such as Spirulina and Chlorella, in Asia. According to organic standards, only organic fertilizers may be used in such a system. Our certified operators claim they use fertilizers such as soybean meal, but we have more and more doubts if that is possible and plausible at all. So far, I have found a few papers confirming that nutrient uptake from organic sources is less efficient. But these papers were based on research combining mineral and organic fertilizers. So far, I have not found any publication confirming it is possible or impossible to work exclusively with organic fertilizers. Maybe the lack of such papers shows that it simply does not make sense and we are on a completely wrong track? Any reference to papers and/or answer from people who have knowledge / experience in this field are most appreciated!
I'm trying to grow scenedesmus obliquus, chlorella vulgaris, scenedesmus sp and chlorella sp.
On Monday I inoculated 4 mL of inoculum in 4 mL of BG11 medium (prepared as 1 mL of BG11 and 999 mL of miliQ water) in separately falcon tubes. I put also agitation. The final mix was very green.
However, on Wednesday, the falcon was so clear and all the microalgae culture was dead.
I'm doing something wrong with the way of growing the microalgae?
Image 1 and 2 (F8 P 100x). Possible diatoms. No idea about ID
Image 2. (F3 P 20x). Preliminary, order Naviculales Gyrosigma sp
Image 3 (FV 40X). Preliminary ID as Glenodinium sp
Image 4 (F9 100X) and Image 5 (imagen1). Same microalgae. I donÂŽt have a clue about it clasification
What is in your experience the best way to kill contaminant cyanobacteria in a green microalgae culture? Antibiotics, and in case which one do you tried?
Have you ever tried to apply variations in the physical or chemical culture conditions?
Let's me know your experience!
Does anyone have experience running microalgae samples in the cytometer? I would be grateful if you could share any advice on avoiding the samples getting stuck in the cytometer lines and clogging the equipment. I use an Attune NxT cytometer, and the samples are from Isocrysis galbana.
I really, really appreciate any help!
I am working on a synthetic biology project in which I am redesigning the pathways in photosynthesis of Synechococcus elongatus to become more efficient, evaluated by increase in biomass. We want to computationally model this in R but we are struggling to find packages that can help us with this. I've looked at deSolve::aquaphy which models C and N assimilation but photosynthesis rate is an input and we want to model this also.
I know from literature that the range is very big like between 80 - 1000 ”mol/(mÂČ*s). My aim is to find a LED light source between the range of 150 - 300 ”mol/(mÂČ*s). When I look on market ther irradiance is often given in LUX and lumen. So how can I convert his value in ”mol/(mÂČ*s). Is there an "easy" equation for it, which I can solve in Excel ?
I need a list of laboratories please, can someone help. Thank you
We will use filtered tap water for large-scale microalgae production. What should be the features of the filtration device? What are the optimal water properties of tap water for the best growth of algae?
To culture asian seabass / barramundi (Lates calcarifer) larvae, we are using rotifers as live feed, needing to be enriched with essential fatty acids (DHA and EPA):
1- Feeding rotifers with microalgae like Chlorella and enrich themby fish oil as DHA source before being fed to fish larvae.
2- Feeding rotifers with DHA and EPA rich microalgae such as Isochrysis galbana and Nannochloropsis oculata (no additional enrichment is needed).
Which one do you recommend and why?
Thank you
Hello,
I am trying to conduct an experiment where I test the effect of different environments on the amount of oil produced from microalgae, more specifically, Chlamydomonas reinhardtii. I was planning on doing a condition wherein which I differ the amounts of nutrients the algae get. I am having trouble finding a growing medium to grow my age, I was wondering if anyone knew how I would be able to get this? Or perhaps know a recipe to make one?
Thank you
Greetings,
we are currently working on the cultivation of the microalgae Dunaliella tertiolecta. We try to count the cells in a Thoma cell counting chamber under the microscrope. Problem is, the cells move phototactically, thus the counting of cells is inaccurate at best, caused by rapid movement of the cells under a light source.
I'm wondering if anyone knows of a method to immobilize the cells? I'd be very grateful for any suggestions!
Best regards and thanks in advance,
Marius Tölle
To know the carbon fixation rate of a microalgae culture, several bibliographic sources use formulas that consider the biomass change between two times and, knowing the carbon fraction of the biomass and with the molar mass of CO2 and C, we can obtain the carbon biofixation rate of a culture.
However, this raises a question. The variation of the algae culture occurs in the growth phase. But if I have an algae culture in a constant maximun biomass for a year (per example), the carbon fixation rate still aplies over that year?
If that volume of culture fixes X gC02 per day, could I estimate with this formula the annual fixation of C02? Or there is another way to stimate the carbon fixation per year of an algal culture of constant biomass
Thanks in advance for your time
I'm starting to work on biofuel from microalgae but I have any idea to collect them with a simple méthod it's a work from my TFC
Question of the day!
Dear Algal Phycologists and Biotechnologists,
I have read the literature about different method of harvesting of Microalgae but I am confused which is the best method as there is no literature up-to-date calming the best harvesting method which is highly efficient. (Flocculation/Sedimentation/Filtration/ Floatation etc.)
Could you please let me know the highly efficient harvesting method up-to-date.
#research #algae #algartech #algaebiotechnology #microalgae #microalgas #cyanobacteria #diatom #algalbiofuel #phycology #phycobiotechnology #algaebiology #marinebiology #marinebiotechnolgy #biomass #energy #environment #engineering #chemistry #sustainability
micro plastic contamination will reduce sunlight penetration and affect the photosynthesis activities and other impact. do have any report or case studies in this regard
IÂŽm growing Scenedesmus sp. and Chlorella sp. inoculum in a f2 culture medium opened in 2012. It's possible that this issue interfere in the growing rate of the microalgae? I noticed that these solution include vitamins, it's possible these vitamins expired?
Thanks for all the answers.
If it is possible, suggest me a protocol, or suggest me a researcher or lab where this kind of research is active. Particularly, the main interest is cell division rate and cell morphology change study under different climatic situations.
Hello,
Research papers are mostly focused on lab-scale lipid extraction which most of them are not cost-effective. Also, old methods such as Folch and Bligh & Dyer are not environmentally friendly.
I wanted to know which lipid extraction method from microalgae is currently available and is being used on the pilot and industrial scale (environmentally friendly and cost-effective)?
Thank you so much,
I have the value of Delta N for 3 producers (water column phytoplankton, sedimented attached microalgae, periphyton), primary consumers (zooplankton), tertiary consumers (different fish species).
How is citric acid beneficial to microalgae growth?
I tried fixing the cells, treatment with 2 % SDS but the dye is not goin inside the cells.
Hello! I am needing some help with HPLC - I had to pick it back up after years of not doing it.
Re-learning has been going well, but I have never had to collect compound fractions and this is what I need help with.
I am performing extracts on microalgae to detect and isolate specific compounds in the UV range. My extract is generated using 100% HPLC -grade Methanol, and my eluents are 100% methanol and DDI water (all compounds have been filtered by 0.22un membrane and degassed via vacuum pump) for my flow gradient. I generated great chromatographs, spectra, etc
BUT, how do I collect specific fractions for analysis? I have multiple peaks at various times that I need to collect. I know that I can manually time it and manually collect the sample, but I need better resolution than a manual collection. I've been looking for manuals and protocols online, but the information for my systems seems to be sporadically available.
Any chemists/biologists/physicists or experienced HPLC users have some tips or resources?
System Information:
Waters 2695 Separations Module w/Column-Heating Cabinet (set to 40*C) - Hardware
Waters 2996 Photodiode Array Detector - Hardware
Waters Fraction Collector II - Hardware
C18 Column - Hardware
Empower 2on a Windows XP OS - Software
There is also a Waters 2424 ELS Detector (Hardware) , but I don't think I need this.
I am interested in anaerobic co-digestion of algae and lignocellulosic biomass, I am uploading the related research paper, with this question.Please gi e your kind suggestions.
I need to prepare bold basal medium for the cultivation of a microalgae Chlorella species, but want to know if I can use something like e.g cobalt chloride as a cobalt nitrate substitute.
After searching among literature for months, I am not still able to choose a microalga strain for my PhD project. The idea is to extract as many as possible valuable compounds (such as pigments, PUFAs, polyphenols, phytosterols, vitamins) from the same microalgal biomass.
To do this, I need a strain that is being already studied and optimal conditions growth for bioproducts target are known. Also, I am not sure that selecting strains already on the market (maybe lipids or biofuels market) could be a good idea.
Which strain do you suggest?
I've found a promising strain (Stichococcus sp. KMMCC 365 but it's impossible to find to buy)
Thanks to everyone that will spend sometimes to reply me
In a typical paper around microalgae biosensor, an increase in current was illustrated with time by chronoamperometry. But, my observations showed a decrease in current with time and I wonder what problem may occur?
Could it be cause of electrochemical method or the kind of microalgae species?
Please share me your experiment and knowledge.
I have delta N for Snail, 3 source organisms (organic matter from phytoplankton; benthic microalgae and peryphyton); primary consumers (zooplankton) and tertiary consumer is Hilsa fish of different sizes.
May I apply TP= [(deltaN primary consumers -deltaN primary producers)/3.4]+1 ( Post DM (2002) Using stable isotopes to estimate trophic position: Models, methods, and assumptions. Ecology 83:703â718)
If so, how can I calculate trophic transfer from primary producers to zooplankton, fish?
Should I use the average delN value of zooplankton as the primary consumer and the average delN value of each primary producers?
We're working on identifying microalgae and macroalgae strains and we plan on doing a PCR later on but we need to exract the DNA first and i can't seem to find methods that don't require CTAB, Liquid nitrogen and phenol:chloroform:isoamylalcohol. We currently do not have these components in the lab.
I am working on a project which requires the cultivation of C.vulgaris in a commercial fertilizer. The phosphorus form stated on the fertilizer's composition list is P2O5. I would like to know if the phosphorus can be utilized.
In my previous understanding, E. gracilis accumulates paramylon when grown in high-carbon medium, especially if grown in the dark. I have reproduced this in the past using KH medium. However, I surprisingly found yesterday that E. gracilis grown in AF6 medium with 12:12 ligh:dark cycles for almost a month showed quite high paramylon content. Can anybody point me towards literature to explain this? Is there any understanding of the dynamics of this accumulation of paramylon?
Hello, I
am trying to store microalgae at 4°C and I want to know if this has no effect on the biochemical composition
I have exposed microalgae cells to temperature stress and measure the ROS level using DCFH, but I found that the DCFH was significantly lower in stress-treated samples compared to control. How is this possible?
Funnily, this is a very prominent topic - yet I wasn't able to come up with a good answer. So here it is: Is there a recipe for a standard nutrient-depleted medium? In many applications, microalgae are starved to promote the build-up of e.g. lipids. One solution is to just let the microalgae stay in the same (exhausted) medium and wait. But what about centrifuging the microalgae and placing them in fresh medium that is poor in nutrients? And if this is done, how should such a medium look like? Suppose we want to make sure that neigher N nor P are available, does it make sense to take a medium recipe and just omit nitrate and phosphate? We would end up with a strange medium. I would rather like to have a typical "freshwater medium with standard osmolarity and no nutrients". How does that look like? Is there a standard recipe available (that is simple to make)? I had a look at artificial lake water, which is rather cumbersome to make, it seems. Is there something simpler?
I've been working on microalgae for some years and still I've not found documents about full scale, operating works for the production of biofuels from microalgae.
I am performing wastewater treatment thought different microorganisms such as microalgae and afterwards determining the lipid content in the biomass though Bligh and dyer method. Due to the COVID restriction, we are allowed to have limited time in the laboratory and therefore, a lot of samples should be stored and tested later.
I have to store my sample for cell counting and lipid determination?
For cell counting, Can I store my sample in ethanol to persevere it and count it later?
(0.3 ml sample and 0.7 ml ethanol)
For lipid: Can I store my liquid sample at -20C. and perform the lipid determination by Bligh and Dyer method later?
We want to determine the photosynthetic rate of freshwater microalgae other than the radioactive method. kindly provide a simple protocol for measurement.
I am currently cultivating C.vulgaris in Bold's Basl Medium. However, even though the medium does not meet the criteria of the Redfield ratio, the culture can still grow.
Redfield ratio C:N:P Ratio (106:16:1)
Bold's Basal Medium (N:P) (1,71 : 1 )