Melatonin - Science topic
A biogenic amine that is found in animals and plants. In mammals, melatonin is produced by the pineal gland. Its secretion increases in darkness and decreases during exposure to light. Melatonin is implicated in the regulation of sleep, mood, and reproduction. Melatonin is also an effective antioxidant.
Questions related to Melatonin
Melatonin and serotonin are easily lost because of their low content and light-sensitive. The extraction steps should be quick. Is there a simple way to extract them from hundreds of plants or any proper extraction buffer recommended? Thanks!
can anyone tell me which is the best way to quantify Melatonin? I've read articles that refer hplc-dad and also hplc-fluorescence. I'm interested in analyze melatonin in raw milk, making the extraction following ISO14156.
Do you have any sugestions ?
Thank you for your help!
Does anyone have any research on - if artificial ingestion (pills/drops) of Melatonin can lead to addiction or lighter dependence? Due to or if, the brain's own production (epiphysis) goes down with extra intake. I.e., In Sweden, melatonin tablets are considered a prescription drug, while other countries classify it as a supplement.
Hello everbody. I just started my first scientif research and i'm having a little bit of trouble converting some units. I'm actually studing the possibily of building a biosensor to melatonine. I'm doing a review of what we already have in the literature about methods of detection, and I wanted to put every detection limit in pg/ml. For example, I found one that has a LD of " 1 fmol per well". I did some conversions (another question: I know the volume of a well is usely 350 uL, but there was this affirmation "the final volume of incubation was 126 uL", so I consider the volume of 126 uL. Am I right?), and found out that the limit were 6,67 pmol/L. Now I'm very confused of what I should do, because I don't have a cleary mass of melatonin (the objeticv of the research was to found that out in humam serum), just that they used 20 ng of melatonin-p-carboxybenzyl-AP conjugate, and I'm not so sure about what to do with this information. I hope this is not a very silly question. Thanks for the attention!
I tried to do a Slot Blot and was not successful and melatonin is only .23 Kda so it would probably run off a western blot. Has anyone had success with other methods?
The protocol used in managing COVID-19 for Mr. president Trump containing:
Regenerons poly-clonal antibody cocktail
what are the differences and advantages by utilizing this protocol?
Recently, I am doing my research on melatonin receptor two (MT2) using bovine granulosa cells. My result show that down-regulation of MT2 resulted in less apoptotic rate in transfected bovine granulosa cells while the control has high apoptotic rate. My result seems strange because melatonin and its membrane receptors have long been known as ati-apoptotic factor. And the apoptotic factors Bax and Bcl2 were up and down-regulated, respectively in MT2 silenced cells. Can anyone comment on this result?
I understand that light is one of the strongest external cues to affect our clock system. I am curious about other possible factors that are less discussed. I am finding a great amount of literature of EMFs on insects such as fruit flies, but when it comes to human studies the results are always contradictory.
I would be open to any suggestions on researchers and the latest studies in the field.
I tried with DI water but it cannot fully dissolve melatonin.Some people suggested to use little ethanol. But i have a confusion that if i use ethanol then it may have interference duringelectrochemical detection.I need some good suggestions.
I need to quantify the amount of melatonin and 6-hydroxymelatonin in a sample. Any suggestion will be appreciated.
Some healthcare providers are reluctant to use melatonin because potential suppression of endogenous production and/or the theoretical potential for pineal gland atrophy.
I am starting a project with melatonin supplementation and would like to make a stock solution for dilution when giving the melatonin to rats; I suppose if I dilute the melatonin in alcohol and store in -20 degrees celsius, it would work, but I am not sure. Couldn´t find any research paper or technical publication on that... Does anyone have an idea?
I'm thinking about using night vision goggles for a series of experiments I plan to perform in the absolute darkness. I understand that some light is emitted from the goggles when used in complete darkness and wondered whether anyone could advise how bright this light is in general? I'm wondering whether the light is going to be disruptive to melatonin levels.
Thanks in advance!
This is for a research protocol in spider monkeys' chronobiology. So far the best saliva extracting device has been an aspirator that allows collecting about 1 ml in 2-8 min. The issue is that most monkeys are unwilling to cooperate if they don't receive a food reward while aspirating the saliva, thus contaminating the sample with the treats used to lure them.
The three analytes will be quantified by ELISA, nevertheless, if someone knew of a similar assay less prone to disturbance by the use of salivary stimulants it would be of great assistance as well. Thanks to anyone that can help me in this matter.
I am interested in analyzing melatonin from human saliva using ELISA kit. There are quite some manufacturers (IBL-international, Amco, salimetrics etc.) that produce such kits but I already heard some negative feedbacks, like a lack of fluctuation in salivary melatonin from ELISA comparing with radioimmunoassay. It would be very interesting to know if anyone has tried any and could give a feedback on which you found useful? Thank you!
I currently have a product that has a small amount of melatonin dissolved in it (aqueous solution), and I need a sufficient amount to get a reading on an IR spec. I was thinking of doing an extraction in a sep funnel using DMSO-does anyone have any resources, links. or experiences with this? Thank you!
Hi! Melatonine is indicated in Europe for sleep disorders in patients > 65 years old .
The secretion of melatonin is regulated by light. Apparently, the following mechanism is observed: absence of light => increase of NE => action on beta1 and alpa-adrenergic receptors of the pineal gland = > activation of N-acetyltransferase => synthesis and release of melatonin (Touitou Y., 2005).
My question is this: When we give the patient melatonin tablets (or a melatonin agonist as the agomelatine), should we be concerned about a potential inhibitory feedback on endogenous melatonin secretion (as is the case for corticosteroids and inhibition of the hyphalamic-pituitary axis): Do we have info about the regulation of this key enzyme, the N-acetyltransferase, by melatonine ? Do cells of pineal gland express melatonin receptors ?
Thank you in advance for your reply :) !
Dear researchers, I want to find some genes related to SOD, POD, CAT, APX, MDA, and melatonin biosynthesis gene under heavy metal stress in cotton or other crops?
Kindly upload the related papers.
To determinefree Melatonin in serum or plasma samples is not so easy as it always found to be bounded to large proteins. Countless methods of the pretreatment of serum/plasma samples are described. But those methods use methanol or dichloromethane to extract melatonin and those are tedious and time consuming. I came to know that some commercial kits use digestive enzyme preferably pepsin for extraction. Does any one know what is the concentration of pepsin to be used or any other chemicals can be used?. I kindly request you to share your ideas or experience. Thank you.
I am looking for melatonin solvent, particularly chloroform,.I found DMF and DMSO concentration but I did not see for chloroform.
I am working on cotton crop. I want to know that which level of Melatonin can improve cotton seedling growth under cadmium stress
Hi, I've been trying to find an antibody against melatonin that workd for immunohistochemistry (i.e., frozen or FA fixed sections of tissues). So far we have found very few commercially available and none that is able to detect free melatonin in our insect brain sections. ¿Does anyone know of a commercially available and not discontinued anti-melatonin? Alternatively, ¿does anyone have an anti-melatonin that works and is willing to send a small aliquote (5µl)?
Thanks in advance,
Dear Colleague (s)
I am having little confusion regarding the best solvent to dissolve melatonin. Among ethanol, isopropanol and water, which will be best keeping in mind the duration of its half-life? I am finding it confusing in Sigma-Aldrich data sheet (Catalogue number # M5250).
Melatonin secretion follows circardian rhythm.. what is the best way to estimate its levels in body? Serum sample, urine or saliva.... whats the most feasible method to follow for a stu in pediatric patients?
I'm looking for either an RIA or ELISA kit that works reliably with mouse plasma. Max blood per sample is 1 mL (500uL plasma). ELISA would be preferred. Any recommendations?
I implanted a number of passerines with melatonin packed silastic capsules (subq). However, the birds showed no increase in plasma melatonin levels at the end of the experiment (implants were in place for 1-1.5months). The implants also still appeared to contain melatonin when explanted (they weren't empty). Anyone have any advice on what might have gone wrong or possible adjustments to make?
Thank you in advance
There is some research indicating possible mitochondrial dysfunction in Psychosis. Currently a client with psychosis has been given melatonin for sleep in combination with therapies done by myself - Cognitive restructuring, talking to voices and cognitive therapy. This was after ALL normal (medical and psychotherapeutic) treatments had been applied for a 2 year period with little success before I received the client. The only two differences in treatment was talking to voices and melatonin prescribed by the doctor. Naturally this is not enough to propose that melatonin may have an impact, however there was a distinct improvement in cognitive processing abilities. Has anyone done any trials or research on the use of melatonin. Studies suggest that melatonin may or may not partially or fully repair mitochondria, whilst no research has been found on the possible utilisation of Gh.
Does any body know if the synthesis of melatonin differs between diurnal and nocturnal mammals? Diurnal species receive and configure their circadian system by interpreting the changes from diurnal to nocturnal levels of light, but what about nocturnal species? Do they follow the same path?
I am curious from the point of view of those who may take sleep-aiding/soporific drugs to hasten adaptation to new time-zones?
Agomelatine is a melatonin (MT1/MT2) receptor agonist and a serotonin 5HT2C antagonist (increasing dopamine and norepinephrine in the frontal cortex). It is an antidepressant and it can also reduce anxiety and re-synchronize circadian rhythms. De Berardis et al (2012, Ann Clin Psychiatry) informed about a female patient with PTSD with substantial improvement after agomelatine treatment. In my clinical practice, I have successfully used agomelatine in the treatment of female patient with PTSD and comorbidity with a psychotic disorder. It is therefore possible that anxiolytic (and many other) properties of agomelatine can help to alleviate symptoms of PTSD.
Is it possible that the value of circadian amplitude higher than circadian MESOR? or depends upon the variable such as physiological (blood pressure) or biochemical (ex. melatonin)?
I mean is melatonin a direct cause in such issues or it is a result? Many papers and reviews reported a decrease in this neurohormone's concentration in patients suffering neurodegenrative diseases!
Could you tell me if light stimulation might be an adequate treatment for sleep-related disorders?
For instance blue light influences melatonin production, what might be helpful to re-entrain the circadian rhythm. Do you know about some light treatment that is nowadays used clinically?
I will be most grateful if someone could give me some information!
Melatonin is derived from amino acid L-tryptophan. Is there any difference between ELISA specificity to the endogenous (naturally occurring) and the exogenous (drug or implants) melatonin?
Hi, I am doing research on light, melatonin and the effects of melatonin on health. I couldn't find any systematic review or recent review on this topic. Can anyone please advise?
As part of a laboratory project, my group and I are studying the effects of cytochrome p450 inhibitors (like insulin, grapefruit juice, etc.) on drug metabolism in Hepa1c1c7 cells. Are there any alternatives to expensive ELISA's that we could use to compare levels of estradiol in these cells? We would also be open to using other substrates such as melatonin, naproxen, caffeine, etc.
Rehabilitation normally consists of different types of therapies, neuropsychological rehabilitation and pharmacological treatments.
Has there been any new developments in the use of stem cell therapy in the hippocampus, use of the endocrine system to buffer against further deterioration (such as the use of melatonin), or other forms of advanced treatment that have occurred or are under way?
I want to give a group of elderly aerobic exercise and melatonin supplements؛ How much and when I complete?
Melatonin and Circadian Typology
What are the causes behind the late secretion (i.e. 2-3 hours) of melatonin hormone in owls as compared to that of larks?
Melatonin signal transduction in skeletal muscle during aerobic exercise and Prevention of DNA damage in The elderly?
I would like to measure the content/activity of tryptophan decarboxylase that is the first enzyme in melatonin biosynthesis catalyzing the conversion of tryptophan to tryptamine. I can currently measure tryptophan, melatonin and tryptamine contents in plant tissues with HPLC fluorescent detection but hard time finding complete method for analysis of this enzyme. Any help on this matter will be greatly appreciated.
I am trying to identify genes involved in the biosynthesis of melatonin in yeast. I am not too sure which is the best approach to use.
We know that melanopsin is sensitive to blue light with a peak around 480 nm and that melatonin suppression is most sensitive to blue light with a peak around 460 nm. Furthermore, it seems that melanopsin is important but not essential to circadian photoentrainment. I have found some older reports showing that circadian phase shifts are more sensitive to short wavelength light.
Does anyone have any idea of how important blue light is to circadian photoentrainment, preferably based on recent research?
Everywhere is clear that melatonin is produced mainly at nighttime, and its production decreases with age almost half from 25 to 50 years old. However the absolute amount of produced melatonin remains unclear for me. I would appreciate very much any information on that. Maximum concentrations in blood in young or old individuals would help also!
In an ongoing study, we have exposed freshwater fish Clarias gariepinus and Giant African Land Snail Archachatina marginata to varying monochomatic lights of different colours - red, blue, green, yellow. Our intention is to establish the physiological impact of light on these animals viz their growth, production of acetylcholinesterase and melatonin. We seek to understand the likely pathway of these impacts and the ecological implications
I am planning to study the effect of melatonin or H2O2 on different treating time periods to the cells, for example, treating at t0 (starting time) and t12 (after 12hr), then collect all samples at t24 (after 24hr).
There two treating solutions: (1) melatonin solution (soluble in ethanol then diluted with complete growth media) and (2) H2O2 solution (diluted with complete growth media), and then store them one or two days at 4 degree C. before treating to adherent cells such as SH-SY5Y. To prepare melatonin and H2O2, a day or two before instead of prepare fresh, could help me reduce workload on the treating day.
My questions are:
(1) Is it ok to prepare melatonin or H2O2 a day or few day, instead of fresh to treat to cells?
(2) If I prepare melatonin or H2O2 stock solution fresh and treat to cells at t0, Can I keep this stock solution at 4degreeC and use it again to treat to cells at t12? Or I should prepare another fresh solution with the same concentration at t12?
Thank you so much
I want to check the effect of melatonin on RAW264.7 cells.I have used ethanol 10 microliter/mL, but this ethanol has some effect and I found the effect of melatonin very few or nothing. So, what solvent and in which concentration will be good for my experiments? Do I need to make molar solution of melatonin or 10, 1, 0.1, 0.01 microgram/mL is ok? Please suggest me.
I want to now how some circadian process change with ageing. For example, the variables like the body temperature, melatonin level exhibit circadian rhythms with characteristic amplitude and time-period. I will like to know whether the amplitude is a constant or does it change with ageing. I have read that melatonin level variations during day and nigh are larger in infants (larger amplitude) as compared to the adults. Is it true for all other circadian processes? What references should I see to understand the variation of the amplitude of the circadian process with ageing. I will be intrested in comparing the circadian processes in children, adults and old people.
I want to work with the macrophage cell line with melatonin. Which solvent and how can I use it for dissolving melatonin minimizing solvents adverse effects? Can you answer elaborately with its required concentration, side effects, preparation method and also something else need to consider for this experiment?
If you have 4 blood samples to measure melatonin, what is the best stat program that can be used to best delineate the rhythm?
BAT = brown adipose tissue
Fexaramine is an investigational compound which acts as an agonist of the farnesoid X receptor (FXR), which is a bile acid-activated nuclear receptor that controls bile-acid synthesis. It induces enteric FGF15, changing the composition of biliary acids, thus enhancing BAT, thermogenesis, and decreasing glucose hepatic production and weight in mice.
See Fang S, et al. Intestinal FXR agonism promotes adipose tissue browning and reduces obesity and insulin resistance. Nat Med. 2015 Jan 5. doi: 10.1038/nm.3760.
I would like to know what is the equivalent concentration of 1 mM melatonin for use in humans. How can I make these calculations?
Several in vitro studies have shown that the pharmacologic concentrations of melatonin is 1 mM, but the physiological concentration in humans is about 70 pM, which is 14,285,714-times lower compared to 1 mM. Please, could you comment this?
1 mM of melatonin equivalent to 0.23 mg/mL
- 20 mg/mL of melatonin equivalent to 86 mM and similarly 1mM of melatonin equivalent to 0.23 mg/mL.
The approximate molecular weight of melatonin is 232,278 g/mole.
So, to convert mg/mL to mM, I divided the concentration in mg/mL (20 mg/mL) by the molecular weight of the sample (232,278 g/mole) and multiplied by 1,000.
If this calculation is incorrect, please, how could I know how many mg are equivalent to 1mM of melatonin?
How melatonin from plants can be used by species such as sheep? There are some papers describing analytical procedures, but which one is more accurate?