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Medicinal Plants - Science topic

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Isolation of endophytic fungi carried out for medicinal plant and started with fermentation for further extraction of secondary metabolites, is design expert is applicable for above mentioned fermentation processe?
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Six different isolation media: plain PDA, plain MEA, PDA supplemented with host plant powder (PHP), PDA supplemented with host plant water extract (PPE), MEA supplemented with host plant powder (MHP) and MEA supplemented with host plant water extract (MPE) were studied and the results showed that the isolated
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He has a research paper on a medical plant aimed at improving the efficiency and productivity of effective compounds that enter as key substances in the pharmaceutical industry.
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Mahdi S. Jaafar yes for sure but this will take some time as I have personally tried this to check the results and believe me the natural biofertilizers enhances the overall soil and its nature
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What types of plant medicines are useful for Paralysis?
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It depends on the nature of the paralysis. For cases of Guilain-Barré Syndrome, homeopathic Conium (Water hemlock in homeopathic potency) has been useful. I cured a case of brachial plexus palsy with Hypericum (St. John's wart in potency). Paralysis due to MS is more complicated and a wide variety of medicine have been use, from plant, animal and mineral sources. Rhus-toxicodendron potentized has been useful for certain cases of paralysis.
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Hello !!!
I am working on the hepatoprotective effect of some medicinal plants and, i want to use the ccl4 induced acute liver injury ( 7days protocole)
there are a plenty of articles that treat the subject, and each one use differente concentration !!
so according to your experience what is the best and most used ccl4 concentration to induce mice acute liver injury ??
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We got the best resalts using 0.5 ml/kg CCl4 to induce fibrosis
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The majority of medicinal plants are obtained from wild resources, making them a worldwide valued resource. Destructive harvesting usually leads to resource exhaustion and even extinction of species. As a result, the long-term usage of medicinal herbs should be considered, as should good harvesting procedures. What techniques and methodologies ensure that medicinal plant resources are conserved and used sustainably?
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In-situ and ex-situ conservation is the best solution for the conservation and sustainable management of medicinal plants but community awareness is also important. In most cases, the medicinal plants are collected by untrained or unprofessional local shepherds, plant collectors, some local traders, and even schoolchildren. They do not know even the plant part used as medicine and active ingredients and collecting/harvesting the plant's irrational ways like if the medicinal part of the plant is leaves, instead of collecting some leaves they cut the whole plants; if the active ingredients or medicinal part of the plant is root then they uproot the plant before seed setting which is the major reason of poor regeneration and sustainability of the plant resource in a certain area. There is thus a need first to identify the people who are involved in plant collection the to provide them training on the proper time of harvesting and collection techniques sustained without degrading the basic resources. There should be a clear-cut government policy on medicinal plant collection and trade following sustainable harvest and management principles.
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I am looking for Herbal and rare medicinal plants including Nardostachys jatamansi for cultivation purpose or else atleast home garden. Kindly guide me to get the seeds. 
Thank in advance. 
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Nardostachys not but Valeriana jatamasi, you can get from Institute of Himalayan Bioresource Technology (IHBT), Palampur
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Can anyone simplify the process of isolating compounds from medicinal plants after crude extracts have been prepared and phytochemical screening has been done. Any guidance will assist.
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Yes, if you are graduated from scientific department.
The process going through the following steps:
1- collection 2-washing with water 3- drying ( under shade) 4-grouding 5- extraction with different solvents( start from non-polar) 6- taking TLC 7- evaporation of solvents 8- separation (using different chromatographic techniques) 9- identification of the single pure compounds(using different spectroscopic methods, IR, NMR, MS,...etc) 10- application in different fields.
Each step has it's detail and following scientific ways.
Finally, you need to join some lectures on phytochemistry and labs.
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which protocol of extraction is best for the maximum extrcation of the secondary metabolites of the medicinal plants . how the extraction yeild is obtained ?
which protocol give clear identification of antibiotic activity of medicinal plants ? agar well diffusion method or disc difussion method ?
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thank you so much all of you for guiding me in best way.as beginner in research i have learnt much.
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An efficient and cost-effective laboratory procedure for obtaining tannin and flavonoids from hairy root cultures
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I am happy to announce that the latest special issue of PCTOC, on Secondary Metabolites and Medicinal Plant Biotechnology, will be in print any day now. Meanwhile you can check the Preface by the editors at https://rdcu.be/cJ2Ryhttps://rdcu.be/cJ2Ry to see its contents. Articles are online already.
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Dear Colleague,
I just tried it and it worked. Are you sure you used the right link?
Please try copy-pasting it in your browser and if it still does not work contact me directly at sergio.ochatt@inrae.fr and I'll send you the pdf by email.
With best wishes,
S.J. Ochatt
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how the plnat extract inhibit the alpha amylase and alpha glycosidase and reduce the diabetic 2 in human body
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Dear Dr Binte Naeem,
The protocols found in this article (in attached) may also help you.
Best wishes,
Sabri
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If possible, active participation for the purpose of collecting the types of drugs used, as well as medicinal plants, each according to their country, mentioning the country, and thank you for your kind cooperation.
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Generally in mild symptomatic infection Paracetamol 650 with Vitamin C , Zinc tablet fir three days ,Prednisolone . For severe acute respiratory syndrome ( SARS-COV-2 ) the patient should be shifted to Hospitals ( ICU management ).
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How can we test the convergence (or divergence) uses of medicinal plants when we conduct a comparative study of different medicinal flora?
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As we know some plants comprise clot lysis properties, for this we are looking for techniques to prove.
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I found this interesting article (in attached) that may help you.
Best wishes,
Sabri
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Is there any successful case of conservation of endangered medicinal plant species? If avilable, please provide me the document.
With regards
Prabhakar
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My team are having an idea to make a cleansing balm combined with some medicinal plants to develop formation for a multifunction cleansing balm, for example we're planning on making the Centella asiatica + Rice bran cleansing balm with the affection of Cleansing + Moisturizing + Antiinflammatory. The big problem we need to face is if it is enough time for these bioactive substances to effectively work on skin? if not, is there any solution to retain them on the structure of skin?
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Hello, I have collected a few articles on this overview for my work. I wish you should take a look at those, that might help you!
However, It's hard to do for cleansing (as it will be washed immediately), But the idea works well if it's a Lotion or gel or cream.
2) DOI:10.3390/nu13010203
3) DOI:10.3390/nu10040403
4) DOI: 10.2174/1381612826666200417160049
Thanks!
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Please suggest to me the names and contacts of Laboratories in India where the Purification of natural products from medicinal plant sources does occur. The laboratories will perform the Purification, characterization, and structure determination of medicinal plant powder, herbal products, or polyherbal formulations.
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Dear Sundar Ray,
Dr. K V Radhakrishnan, Dr. L. Ravisankar and Dr. B. Sasidhar at CSIR – National Institute for Interdisciplinary Science and Technology (NIIST), Trivendrum; Dr. K. Suresh Babu at CSIR - Indian Institute of Chemical Technology (IICT) are helpful about your search.
My self (Dr. K. Venkateswarlu) and Prof. A. G. Damu at Yogi Vemana University, Kadapa, Andhra Pradesh were also have good experience in separation, purification and structure elucidation of natural products.
Please let me know about your requirement, so that I may help/suggest you further information.
Regards,
Dr. Katta Venkateswarlu.
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I need someone who understand results from Triple TOF LC/MS/MS of medicinal plant extract, who will assist in interpreting and my results and may wish to be a co-author with me when publishing my findings (which include molecular dockings)
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I can help you in this field
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Hi, Everyone
I want to check the secondary metabolites of endophytes whether endophytes have similar phytochemicals or not. I have lots of endophytes isolated from medicinal plants. Kindly suggest to me what should I do?
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First establish , whether any important endophytes present , if they are present , in which plant part , such endophytes exist , such preliminary information is mandatory to go ahead..
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Is there a scientific journal concerned with biology, microbiology, biotechnologies or medicinal plants, issued monthly or every two months, and is ifree of charge?
Is there an accredited scientific journal concerned with biology, microbiology, biotechnologies or medicinal plants, issued monthly or every two months, and is it free of charge?
With thanks
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With an Impact Factor of 4.300, the journal is the 5th most cited open-access journal in the Microbiology category . Overall, with journals listed across 20 JCR categories, Frontiers' Impact Factors are consistently ranked among the best performing journals, ranking as high as at the 96th percentile.
Thank you
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See above
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Caffeine does not behave as base. It belongs to alkaloids mostly due to its pharmacological action.
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what is the aim of centrifugation in reducing power assay and is centrifugation required for a standard also [ Ascorbic acid]
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I tried to cover the bitterness of the bioactive compounds of a medicinal plant by encapsulating them using the nanophytosome technique putting them in a sweet food product, but it did not work.. Please suggest an aprroved way to overlap this problem for the food product or the bioactive compounds phytosome.
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I am doing research on growing rare medicinal plants using vertical farming technology, I have a few questions regarding it,
1. Is it possible to grow the medicinal plants in vertical farms?
2. How well is the medicinal value of the plants that grow in the vertical farms?
3. Any researches stating it showed a better result in vertical farms?
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Very interesting, take a look at this video https://www.youtube.com/watch?v=3Ww2TP_tU7o
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I´m searching for the name of this flower. I thought that it was a kind of trifolium pratense but I really am not sure and appreciate your help!
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I would like to synthesis of ZnO and Ago from medicinal plants, I look for the best method for synthesis of nanoparticles
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I agree with Dr J. C. Tarafdar.
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Here in Pucallpa, Ucayali-Peru region, our native communities are suffering from the Covid-19 attack, before which they are using their ancient knowledge of the use of medicinal plants to mitigate their devastating effect. However, living from receptive tourism, today they distrust outsiders or anyone who comes to their communities, therefore, it is urgent to develop strategies to insert them again in the theme of generating economic income through experiential tourism.
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Totally agree, the use of medicinal plants is gaining ground in the fight against Covid-19, we must deepen research on the use of medicinal plants by indigenous populations.
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Endophytic fungi are ubiquitous to plants and are mainly members of Ascomycota or their mitosporic fungi, and some taxa of Basidiomycota, Zygomycota, and Oomycota. These endophytic fungi reside within living tissues of the host and establish symbiotic relationships . The colonization of plants by these fungi varies considerably; and some medicinal plants are reported to harbor more endophytes . The endophytes associated with medicinal plants are known to acquire the potential to produce the active principles for which the host is known .They are a rich source of functional secondary metabolites that include flavonoids, terpenoids, steroids, phenol, phenyl propanoids, quinines, indole derivatives, amines, alkaloids, amides, pyrrolizidines, sesquiterpenes, diterpenes, lignans, isocoumarinated chloride derivatives, metabolites, phenolic derivatives , aliphatic compounds.
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We have a paper in press about endophytic fungus Saccharomyces cerevisiae in medicinal plant and see that this yeast idinduces the plant to produce phenol, quinines and other importnat secondary metabolites.
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Normally it was observed that plant grow in cold climate had produced bigger amount of resveratrol. Whereas further research finding indicate a surprising conclusion that it is because, in cold climate the plants are more susceptible for fungal infection. Therefore plants produce resveratrol to prevent itself against the fungal infection.
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I am particularly interested in medicinal plants used in viral and bacterial infections, repiratory problems and gastrointestinal problems. Artemisia sp. particularly A. vulgaris is very widely distributed as wild plant in Nepal. hence I have this question. Thanks a lot for your valuable time.
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Have a look at this useful RG link.
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The IMPPAT database is a compilation of Indian medicinal plants. But there is not an option to download all phytochemicals together. Is there any way to download like that ?
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Dear
I think you can simply download phytochemicals from the PubChem (https://pubchem.ncbi.nlm.nih.gov/ ), where it is freely available with different interesting options too.
I think together would not be possible to access it, because it will demand you for charges.
Thanks
Regards,
Asif
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... such as Ziziphora, Alyssum, Linum and ... genus.
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Your welcome Dr Ali.
I wish you all the best in your research.
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I want to write a review article in medicinal plant chemistry...please suggest a good topic to write a review and also please guide me as to how many years research papers i should go through on that topic, and will google scholar be enough for my review article writing,..waiting for your precious suggestions...thank you in advance
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We are performing an antidiabetic assay by inducing alloxan in rats and we have to share with metformin, glibenclamide, or actrapid for the positive control.
Which of these three drugs is the best to use?
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Dear Nantenaina, thank you for sharing this very interesting technical question with the RG community. For streptozotocin-induced hyperglycemia please have a look at the following potentially useful article:
Glibenclamide or Metformin Combined with Honey Improves Glycemic Control in Streptozotocin-Induced Diabetic Rats
(please see the attached pdf file)
Also see the following interesting paper: Comparative effects of glibenclamide, metformin and insulin on fetal pancreatic histology and maternal blood glucose in pregnant streptozotocin-induced diabetic rats
This article has been posted by the authors as public full text on RG. Thus you can freely download it as pdf file. Good luck with your research and best wishes!
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If yes then what can be the possible nutrient media protocol for conducting such experiment? To conduct an experiment to see the root and shoot vigorousness of a succulent medicinal plant, I need an protocol to conduct the experiment. Kindly suggest some..
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Yes, you can grow.
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Hello,
I'm wondering whether medicinal plants could be given directly , i mean without using its extracts , to lab animals , like seeds or leaves mixed with food, is it possible using it (medicinal plant) like its natural state, i mean scientifically possible ?? Is their anything in literature some studies of medicinal plants effects using the whole seed or leaves without extracts???
Thank you
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Si es posible utilizar plantas en vivo.
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My job is to extract, separate, purify and identify phytochemicals, I need lots of cooperators to find out their potential bioactivities.
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Dear Wenliang Xu,
Could you describe more details about the medicinal plant extracts which you have had available? Please a attached PDF file (name, quantity, purity, and so on), it will help everyone support to you better.
Best regards
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I want to publish a review article on the anticancer properties of some wild and medicinal plants. Kindly suggest some journals having good indexing and publish the article either free of cost or at low cost.
Thanks & Regards
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I dried the leaves of basil in the oven at 70 ° C. Can I use them for essential oils extraction?
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Currently, many medicinal plants that have toxic effects are observed, any article that presents the secondary metabolites involved?
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I am doing research on the antidiabetic and antilipidemic effects of some traditional medicinal plants. In this regard, some of the parameters to be studied are the glycated hemoglobin level, insulin level, and hexokinase, glucose-6-phosphatase, and fructose 1-6-bisphosphatase enzymes.
To do so, i want to use spectrophotometer method with standard kits. however, i could not get any detail procedure except very shallow information.
i will be very much happy if any professional help is forwarded.
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Search for discovery of new compounds is an ongoing process for drug discovery. Medicinal plants are a rich source of producing secondary metabolites. Normally natural products chemists prefer to isolate compounds from higher plants as compared to herbs.
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As per literature review it is found that some medicinal plant parts consumed by ethnic people of specific location to heal specific diseases but in other location it has been used differently. why?
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Depends on the plant species and their photoperiodism, i.e., is a certain plant species day length neutral or requires a "long" day or maybe "short" day. Under the term of day, I mean the daylight length, I hope you understand what I meant, such phenomena are called photoperiodism. However, it is also well-known that not only the day length but even the spectral content of sunlight has a strong influence on accumulation of phytochemicals and not only in fruits, but also in leaves. Please see the paper under the following link:
Also, the geographic position has a strong influence on accumulation of total essential oils in some plant species for instance in bay laurel (Laurus nobilis L.) please see, one of my papers under the following link:
You can download the full paper in PDF and option for downloading is below the keywords. Sincerely, S. Srečec
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Is it possible for articles dealing with these topics? Thank you
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I am currently working on phytochimistry I'm looking for some new interested plants that I want to do my research in.
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Please let me know the best condition for the drying/dehydration of Medicinal Plants prior to extraction?
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It depends on your herb, shed drying may be good for medicinal plants,and you can also go with oven drying.
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I am starting a study and I need general information about natural compounds.
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A few other databases of potential interest:
Pytochem: This site is structured around the plant species and identifies notable compounds in that species: https://phytochem.nal.usda.gov/phytochem/search
Phytohub: This site focuses on dietary phytochemicals and metabolites: http://phytohub.eu/
PDT: This site is a structural database for phytochemicals: https://pdt.biogem.org/
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Why it is important to study effect of heavy metal in medicinal plants?
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I am currently developing studies that consider the accumulation of metals in different organs, I use M. oleifera as a biological mole, and I share my consideration with you, there are plants that are bioaccumulative, that is, that incorporating heavy metals into their tissues does not cause alterations in its physiology, growth or development, rather, so in the study of the accumulation of heavy metals, a group of variables and research interest must be considered, focused on: If you want to validate bioaccumulation with a remedial approach for environments affected by heavy metals, or the cultivation of the plant in environments with a history of contamination by the aforementioned metals, and the destination of the culture is to feed man or animals, likewise it must consider that the mere presence of a heavy metal does not mean that it is incorporated to the plant, because it is the valence state of the plant that triggers the toxicity. Best regards
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Hi,
Sorry if any of you found this question a bit silly. But, May I know, if I am doing an investigation on medicinal plant and would want to know its antioxidant activity using DPPH scavenging activity (Note:the extract was prepared by dissolving 2g of dried plant in 20 ml solvent):
1) Is my crude extract was considered to have a concentration of 0.1g/ml (since in many DPPH protocols, need to have a serial concentration of samples tested)?
2) So, if it is considered as 0.1g/ml, then, my dilution should be from this point right?
3) Usually, what is the blank, the control used in DPPH scavenging activity? As I have seen some differences about the blank and the control, as well as equation used in literatures.
Thank you in advance
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How to treat auto-immune diseases naturally, using plants or natural products, with the reference?
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Have a look at the following RG link.
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Is it safe to isolate important metabolites from medicinal plants grown in metal contaminated soil? I am checking the effect of metals on plant growth, morphology biochemical variation, and important metabolite production
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Sample preparation for mercury requires special attention. Losses of mercury up to 90% are possible.
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Any one interested to work in collaboration on medicinal plants as antivirals and as candidate plants to cure Covid-19?
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I want to work in collaboration
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I am attaching the photos of a Rubiaceae member for identification which I have taken from Oddanchatram, Dindigul district of Tamil Nadu, India.
I request the specialists to identify the plant.
Thank you.
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It looks close to Oldenlandia corymbosa L.
Thanks!
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Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis and development of medicine/drug. Using the applications of Remote Sensing and GIS, can someone tell me if its possible to model and map the availability of medicinal plants within our locality, if so what and where should I get the needed data? Or should I be more specific as to what medicinal plant will I make use of? This topic is really interesting for me, this would be my thesis proposal this sem. If anyone can suggest/recommend or give me an insight about this, I would be really glad. Thank you.
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Dear Jeila Marie Jagna There is different modeling techniques in combination with Remote Sensing and GIS for plants and animals. The choice of a specific technique depends on the researcher and the knowledge of using the technique. To date, I have used MaxEnt (Maximum Entropy), Spatial Multicriteria Evaluation (Analytic Hierarchy Process), etc. using ILWIS and ArcGIS software for plants like Taxus baccata, Rubia cordifolia, Malus domestica, and Camellia sinensis. Further, I have submitted a manuscript on the modeling of Bos frontalis using MaxEnt for possible publication. It is good to learn that you are interested in mapping the available medicinal plants in your locality by applying Remote sensing and GIS. So, you can go through my publications and if you need further assistance then let me know.
Best wishes,
Dr. Gibji Nimasow
Itanagar, India
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Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis and development of medicine/drug. Using the applications of Remote Sensing and GIS, can someone tell me if its possible to model and map the availability of medicinal plants within our locality, if so what and where should I get the needed data? Or should I be more specific as to what medicinal plant will I make use of? This topic is really interesting for me, this would be my thesis proposal this sem. If anyone can suggest/recommend or give me an insight about this, I would be really glad. Thank you.
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Hi jeila
From your explanation, i think that the best approach for your study is to use suitability modeling or species distribution modelling aporoach. It's more apropriate and need you to collect some sampling data
You can find a lot of litterature and tutorial on how to use it or implement it using software like R on Google
Hope it'll help
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Numerous scientific reports cited plant extracts with potential antiviral activity and even anti-COVID-19? Why these findings are neglected?
I remember very well the warning released by the WHO to the developing countries at the beginning of this pandemic and its expectation that this pandemic will infect hundreds of millions of Africans because they lack facilities and good health services!!
Right now, the developed countries are within the top 10 most affected countries!!
Is that attributed to the fact that more than 80% of people from developing countries rely on medicinal plants (From WHO reports)????
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Well, I do not know if medicinal plants can tackle or completely cure Covid-19, but there are medicinal plants which are capable of suppressing/inhibiting some symptoms of the disease
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The solvents I want to use are: Petroleum ether, Diethyl ether, n-butanol, Chloroform, Ethyl acetate, Acetone, Methanol and water.
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Helpful
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A traditional medicine (e.g. Unani System of Medicine) has its own philosophy of treatment of a disease. Nowadays, there is emerging demand to validate the effectiveness of an intervention (a plant medicine or traditional procedure) in terms of science and evidence set as standard. there is a little focus on the philosophy applied for the therapeutic purpose.
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Traditional medicine is the ancient and culture–bound medical practice which existed in human societies before the application of modern science to health. The practice of traditional medicine varies widely, in keeping with the societal and cultural heritage of different countries. Every human community responds to the challenge of maintaining health and treating diseases by developing a medical system. Thus, traditional medicine has been practised to some degree in all cultures.
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Cigugur Traditional Village have abundant natural resource potential for medicinal plants. The abundance of medicinal plant natural resources is characterized by the number of plants used by traditional people as medicinal plants, but there has not been much research on the ethnobotany of medicinal plants in the traditional village. This study aims to determine the types of medicinal plants used by the Cigugur traditional people. Ethnobotany data were collected by interview method. The selection of respondents was done by purposive sampling technique, , the number of respondents collected was 13
people of different professions and ages. There were 115 types of medicinal plants obtained, with a
utilization percentage of 35% for external wounds medicine, 29% for internal medicine, 14% for gastrointestinal medicine, 10% for excretion medicine, 9% for respiratory medicine and 4% for fever
medication. The Antanan plant (Centella asiatica) is a type of medicinal plant that is most widely known by the traditional people with an RFC index of 0.76. Knowledge of the types medicinal plants of the
traditional people influenced by age and professional factors.
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Thank you for comments and advice, I totally agree with statement limited respondents, because I don't have much time to search a lot of informants.
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I really need help. I tried finding a protocol on how to prepare samples for gene expression . I am working on Antimicrobial activity of a medicinal plant and I would like to check its effect on virulence genes. But i am unable to find a protocol on how to prepare my bacteria and fungi by incubating with and without extracts in order for me to isolate total RNA.
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If we extract the essential oil from a medicinal plant i.e., Ajwain seeds by hydro-distillation technique, it will yield about 2-3% essential oil. My question is about the remaining 97-98% portion of the ajwain seeds after extraction of essential oil. How we can utilize this major fraction in an efficient way?
Please guide me in this aspect.
Thanks and Regards
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Oh ok! I can't think about anything. Did you do an analysis to see if stil have medicinal properties?
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Most of the current existing plants are the result of an adaptation of plants to various factors, especially the environment, of course, there are small portions of species or cultivar plants produced by humans. This evolutionary and adaptation process results in various species of plants growing only in certain geographic environments, where could be owned by certain countries based on the territory. A country where certain plants have grown may claim that this particular endemic plant belongs to the concerned country. Please give an opinion, whether this claim can be justified or not? Is DNA barcoding needed to be legal evidence to support the claim concerning those certain plants?
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It is a very important step, if implemented in India. There are more duplicacy in the variety exist in the country.
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Is there any mechanism or relationship between Methyl dihydrojasmonate and secondary metabolite production? Especially when it comes to medicinal plants that members of Asteraceae family.
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Hi dear Nisit Kittipongpatana, thanks for the article. Best Regards.
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I am interested to learn about remote sensing with medicinal plant. How can I apply the remote sensing with medicinal plant and also how can I manage the insect pests and diseases of medicinal plants?
In this research, I am interested to analysis the PCA, Meta, and also trying to set a Correlation and Regression.
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Please see the following link it may be useful:
Evaluation of Five Medicinal Plant Extracts on Aphis craccivora (Hemiptera: Aphididae) and Its Predator, Chrysoperla carnea (Neuroptera: Chrysopidae) under Laboratory Conditions.
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Dear colleagues,
I am a lecturer from Tra Vinh University, Viet Nam.
I am seeking a partner from Switzerland to apply the project to the program called New bilateral program with Vietnam.
My working area is about pharmacognosy and medicinal plant.
Thank you so much for your help.
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Thank you very much Prof. Walid Moghith.
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Ethnomedicinal practices useful in the treatment of acne
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Turmeric and aloe Vera
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Hi,
My question is regarding medicinal mushroom extraction, but may also apply to broader extraction knowledge of all biota.
I understand, that water and ethanol as extraction solvents (along with many other solvents) will yield a variety of bioactive compounds under different extraction conditions.
In many cases, studies combine the use of solvents in order to refine extracts or increase isolation of desirable compounds. A common conventional extraction method might look like a 2 hour submersion in hot water followed by a 2 hour submersion in 60% ethanol, for example.
My question is regarding this order of extraction solvents used: does the order of solvent used truly matter, from a biochemical perspective?
Li et al (2019) extracted polysaccharide from Grifola Frondosa with an extraction order of ethanol at 95%, then 55%, then hot water, using the same precipitate material.
Whereas Svagelj et al (2012) also extracted polysaccharide from Grifola Frondosa, but used hot water first, then followed by ethanol at 45% and then 90%, using the same precipitate/filtrate material.
These two studies provide a nice example of where my question comes from, as the order of solvents used in extraction is reversed, although the aim is similar (unless Li et al (2019)'s targeting of heteropolysaccharide requires this extraction order, which I highly doubt and it is not stated in the paper).
Moreover, most studies do not cite or mention why this order is chosen.
My assumption would be that it does not matter the order of solvents used, but part of me thinks that alcohol, when used first, may break-down water-insoluble bonds in the mushroom material, allowing the subsequent water extraction (and/or other extractions) to be more efficient.
There are numerous papers that do utilise an alcoholic pre-soak/wash prior to extractions. Again, the reasoning why is never stated.
However, maybe when using modern methods, such as ultrasonic-assisted extraction, the order no longer matters?
Any input would help clear this up. I am sure it is basic chemistry, although I am not a chemist! :)
Thanks
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The full extraction methodology they employed is as follows:
Dried Grifola frondosa was chopped by an electric grinder and sieved through an 80 mesh to prepare dry Grifola frondosa powder. Grifola frondosa powder (100 g) was pretreated 5 times with (v/w) 95% ethanol using ultrasonic extraction (300 W, 50 °C) for 60 min. The precipitate was extracted 5 times with (v/w) 55% ethanol using ultrasonic extraction (300 W, 50 °C) for 60 min. Subsequently, the precipitate was extracted 10 times with hot water (v/w) using ultrasonic extraction (300 W, 80 °C) for 60 min. The supernatant was again precipitated with the addition of cold absolute ethanol to a final concentration of 80% (v/v) and kept at 4 °C overnight. The resulting precipitation was vacuum concentrated and freeze dried, yielding Grifola frondosa heteropolysaccharide (GFP).
As I understand the pre-treatment with ethanol is used to "soften" the cellular matrix before the extraction with water, as you said. Because at the last step it uses that supernatant aqueous and precipitates from there to the heteropolysaccharide with alcohol to 80% and low temperature during the whole night.
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I am gonna explore the phytochemical analysis of those medicinal plants that are novel or traditional as well as a bit of work was done.
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Peonia emodi
Bergenia ciliata
Podophyllum emodi
Skimmia laureola
Berberis lycium
Taxus wallichiana
Withania somnifera
Withania cogulens
etc
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All contributions are highly appreciated.
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DPPH inhibition (%)={(A0 –A1)/A0}×100 where A0 is the absorbance of control and A1 is the absorbance of test.
For standard test 1.0 mL of various ascorbic acid solutions (100 μg/mL) and gallic acid solutions (10 μg/mL) were used in place of sample extract.
In DPPH free radical scavenging method, IC50 (Half maximal Inhibitory Concentration) value is the concentration of the sample that could scavenge 50% of DPPH free radical.
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Plz provide chemicals that are used in antioxidant tests. 
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Dear Experts.....I am doing the superoxide radical scavenging assay.The result I got was opposite to what I found in theory/literature. I used NADH-PMS system. According to literature, the reaction must give the blue formazan dye.When I add the antioxidant standard to the reaction mixture, the color intensity should decrease.But I got the opposite result like the NADH-PMS-NBT system doesn't give any color formation. But when I add the ascorbic acid to the system, it showed blue color formation. But for the medicinal plants extracts, color doesn't change.Please give me any advice or show me how to correct the protocol.
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Mohammed Junaid Hussain
I will send you the protocol I used.
Best Regards,
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Recently I have collected eriophyid mites from leaf erineum of a medicinal plant. When I prepare slide in Hoyer's medium, it is not getting cleared fully, instead I could see fine oil globblets on the entire body surface. I could see only the body margin clearly and difficult to study/keyout using surface structure. Out nearly more than 50 specimens, hardly one specimen was fairly OK and I could make out characters upto generic level, with difficulty. It belongs to Aceria genus and further study has not been possible. (Presently we are working on the integrative taxonomy of mites; for mprphological data we are supplementing DNA data using COI gene/marker) GenBank matching in Blast analysis shows maximum matching of 82% with Aceria genus .
Can any one suggest me to get clear eriophyid specimens. The same Hoyer's medium is being used for tetranychid, tarsonemid, phytoseiid and other familly mites, with absolutely no problem and getting very clear specimens. Even with Aceria mangiferae, A. cajani and other common Aceria species this problem of specimen clearing is not noticed.
Pl. do comment on my problem and suggest me corrective measure......
From
Srinivas
UAS, Bangalore, Karnataka, India
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Dear Srinivas,
The best suggestion has been suggested by Professor Seeman. You should clear the specimens before mounting slides. Keifer's procedure can be difficult for whom it is not an Eriophyidologist, please read that important and the best article regarding this issue (recommended procedures and techniques for morphological studies of Eriophyoidea (Acari: Prostigmata) de lillo et al. 2010).
If you want to prepare slides with Hoyer's medium or Heinz's medium, I have a suggestion for you: put the specimens to Lactic acid for 3 or 7 days in room temperature or Lactophenol (be attention, it is toxic) after that you can mount the slides (you should drown the mites into liquids, if them mites were in the surface the liquids it can be not clear)
Be careful, for identify the Eriophyid mites you should have the female specimens in Dorsal and Lateral position (at least 10 clear specimens) and also if you have nymphs or males, you will can prove the Host-specify.
Kind regards
Arash Honarmand
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I want to perform HPLC analysis of medicinal plants
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Dear Kush Pankaj Kushwaha,
It is also important to know whether you are going to extract only soluble extracts or bound extracts where the extraction solvent and treatment is a bit different.
Please, consult:
Gonzales, G. B., Smagghe, G., Raes, K., & Van Camp, J. (2014). Combined alkaline hydrolysis and ultrasound-assisted extraction for the release of nonextractable phenolics from cauliflower (Brassica oleracea var. botrytis) waste. Journal of Agricultural and Food Chemistry, 62(15), 3371–3376.
This will help you to get a reliable data, say, if you are going to deal with the profiling of different phenolic compounds in soluble and bound extracts and utlimately their total.
Good Luck!
Tilahun
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I've heard garlic and high nitrate foods like arugula or beets typically occupy high ranking here. What others do you know of or recommend? What's your top 10 list, based on what research? Thanks.
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When I eat lemon, lower my blood pressure
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I have tried olive oil as a vehicle but the extract is not also soluble. In fact the extract dissolves in ethanol but crystallizes out on addition of water
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Not more than 1% DMSO
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I am currently doing my research on a paper on secondary metabolites of medicinal plants  I am having a hard time finding a good reference for the Rf factors of the different compounds which are common in plants (e.g. alkaloids, terpene). I only used thin layer chromatography (TLC) for the separation of the different secondary metabolites. Are there any references that you can suggest that I use for this paper? 
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I think no reference can provide you with the required information. This is because the presence of many mobile phase systems as well as the diversity of the compounds that belong to each type of the phytochemical classes.
All the best
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I ask about the classification of this plant, it is a wild parasite that grows in Algeria.
The plant belongs to Cistanche genus , but I would like to know the specie??
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following