Questions related to Medical Entomology
I am looking for a post-doctoral fellowship in medical Entomology (particularly lepidoptera). Please let me know if you have any suggestions.
I will be rearing a colony of wild-caught Aedes albopictus at a remote field site where access to, and storage of fresh blood for artificial feeders will be inconvenient. It has been suggested to me that I should live feed the colony using domestic chicks (which we could safely/humanely house at the field sites), however I've failed to find a single protocol that describes this method. Has anyone used this method before, or know of a published protocol?
DNA from Bartonella, a gram-negative bacteria, have been found in mite droppings, and gram-bacteria like rods have been identified 'budding' off the walls of the mite's gut. Positive identification is needed as the endotoxin from the bacteria may be one of the many allergens from the mite Dermatophagoides.
I have conflicting information about the methods used to raise Anopheles funestus mosquitoes in the lab. Has anyone successfully managed this? Would you please share your experiences and/or protocols?
A 1969 paper reports prevalence of Zika virus in Malaysia (Marchette NJ, Garcia R, Rudnick A (1969) Isolation of Zika virus from Aedes aegypti mosquitoes in Malaysia. Am J Trop Med Hyg 18: 411–415).
During off the season/winter season, what should be our best strategies for dengue vector control to prevent from next epidemic season? I think Integrated vector management (IVM) strategies should be used round the year. We should continuously use ovitraps to check for seasonal trends, increase or decrease in vector population so that timely use of conventional control methods could be applied. Ovicides like bleach and some botanical extracts might be beneficial. But kindly share your ideas and experiences?
I am looking to collect adult Ceratopogonidae in SE Australia, targeting species with aquatic larvae. Can anyone suggest good trapping methods? I know people used malaise traps before. Would you use them overnight or during the day?
Pregnant women and children under the age of five years remain disproportionately affected by malaria. Roll Back Malaria points to the fact that a child dies every minute from this preventable and curable disease.
I am working with A. aegypti but there was a problem with the breeding and the colony has not recovered.
When I worked by LPT Method on mentioned ticks, mortality in control groups was above 20% (in the closed Insectarium (3*3*2 meter) with temperature between 27-28 centigrades and RH between 70-80%), whereas in other ticks such as Boophilus annulatus and Rhipicephalus bursa in this condition by LPT method mortality in control groups was between 0-10%. Why?
The identification of Culicidae by morphological characters takes into account very small structures. Which increase is the ideal to do identification of Culicidae? Does anyone have any suggestion of equipment with a good cost benefit?
Comparing the identification of Culicidae through morphological characters with identification by molecular biology we can initially talk about cost. In addition some other points can be highlighted: the experience of the one who makes the identification and the cost of the tests and euipamentos. In practice, which is better? What are the advantages and disadvantages of these two methods?
The mosquito has an appetizing flight toward attractive events such as chemicals and thermals. Added to this, could it see the light events and thus have improved his direction to the target?
We know that Aedes aegypti is an extremely well adapted species. Its control is very difficult (or impossible). Many are the factors responsible for your success. I would like to know the biggest challenge for the control of Aedes aegypti in your opinion.
In our study we're tracking Japanese encephalitis Virus (JEV) through its animal host, the pig, in Oriental Mindoro. We've come across this study done by Lindahl et al. (2013) which say that Japanese encephalitis which is usually asymptomatic, can be determined in sows who abort or have stillborns. Is this always the case? and how can we make sure that it is caused by JEV?
I am writing a research paper on dengue, a disease transmitted by mosquitoes, and the areas where it is most common and are populated by humans. I believe that to discover the most common areas accurately i would need to use a certain aspect of the mosquitoes that can be efficiently quantified, such as the type of blood that mosquitoes prefer.
Does there anyone know how to sexually distinguish the 4th-instar larva of Aedes albopictus by morphological feature?
There are specific arthropod vectors for specific diseases. Leismania is carried by Sand fly, malaria by Anophelis mosquito, yellow fever-dengue by Aedes mosquito. Why other vectors can't transmit the different parasites or virus. Particularly Culex or Aedes can't transmit malaria. May it help to control the transmission If we can detect the factor of the respective vector transmitting a disease.
I and another student are writing a research proposal, entitled "The Impacts of The Interspecific Competition Between Aedes aegypti Linnaeus 1762 and Aedes albopictus Skuse 1894 on The Spread of the Dengue Virus". We will test the hypothesis that the competitive abilities of both species will be affected by the current season in the Philippines (cold, rainy, and hot). We are also considering the introduction of Wolbachia infection to both species as a method of reducing their populations since these mosquitoes serve as vectors for viruses such as the dengue virus.
My questions are divided into two different parts:
1) There are a lot of mosquito control products int he market. I am currently working with one company dedicated to mosquito control, as everyone discusses the new sciences eg. genetic engineering or new larvicide or adulticide but I am interested in knowing the real business after the first testing is done.Kindly suggest some papers.
2) I have been thrown into a question that what if I have been given 10 Million dollars and preliminary results from a potentially applicable model for mosquito control, so how I am going to use those 10 Million dollars, should I get convinced by the preliminary results and go for further testing or should straight away not get convinced by the results and go with other projects.
I am doing a research on ticks (Rhipicephalus microplus). I want to set up an apparatus where I can feed the experimental ticks with fresh blood. Any help will be appreciated.
Am working on these three species and need to know the identification procedure for these. Both adult and larvae. Also, enlighten about instar stage identification. Thank you
How to properly explain sampling of aedes larvae from big container and small container? Need to have proper documented guidelines for citation. Thank you so much
Myiasis is the invasion of host tissue, but is there active blood-feeding, or is there another source of nutrition for the maggots in order to develop through their instars
I am researching Trichopterans that are developing in the Colorado River. For the past few years the population has reached nuicance levels that are having a negative economic effect for 5-6 months out of the year. Any thoughts as to what could cause a massive population increase with these insects? The primary species is Smicridea fasciatella. There seems to be an increase in algae that could be providing nutrients. There is also a reduction in the amount of trout that are stocked. Any thoughts would be welcome, thanks!
I tried looking up articles where people used FBS and glycerol to induce salivation in ticks, mosquitos, and mites, but most of the articles don't mention exact volumes used. Most just say 50%FBS/50% glycerol. Has anyone done any research on honey bees and varroa mites? Any help would be greatly appreciated!
The two techniques used to evaluate the systemic action of the insecticide: 1) an artificial feeding device with blood-insecticide mixture from which fleas fed on and 2) rodents fed on insecticide-treated bait on which fleas take their blood meal.
This is probably a very naive question but to date I haven't obtained a satisfactory answer. I often must study old papers. I recurrently find these old papers very reticent and short in some of the most respected journals of today, e.g. Science and Nature. Some of those are highly cited papers taken as paradigms in specific fields.
I herein include an example from my field, ant venoms. Anyone working on fire ant venom has bumped into the paper below:
"Chemical, Insecticidal, and Antibiotic Properties of Fire Ant Venom"
Unfortunately the main author has recently passed away so I cannot ask him this specific detail now: where is the data and methods description?
All results as reported are central to my field of study, but the narrative is too short to allow understanding any details. I though maybe details are presented elsewhere in the edition, but it does not look like from seeing their website.
Perhaps someone more experienced could give me a clue there, please?
Thanks in advance
As I'm working on some biological aspects for medicinal maggots, I need to represent my data (e.g. larval density, food type, impact of previous parameters on the development, etc) in a new statistical form to be expressive and useful. Anyone can help me with ideas as I was quite distant from this field?
I am currently looking for pure products of Tyrophagus putrescentiae and thyreophagus entomophagus as pure products.
Is there anyone that can give some advice on how to quickly differentiate (morphologically) the adult females of Anopheles gambiae sensu lato from those of Anopheles funestus in the field? Is there a particular feature to look for without using complex identification keys?
We are studying Malaria drugs resistance, and we found that the % of early treatment failure in P. falciparum infected patients treated with ACT higher among children below 5 years.
Is this normal or explanable ?
I have been having challenges with obtaining all the reagents for the blood meal origin analysis of mosquitoes from one single source. I prefer to get the reagents from one source because of compatibility issues.
In other genus of Culicidae family (e.g. Culiseta, Anopheles, ....) is cannibalism confirmed. Usualy, the younger larvae became a prey for older larval instars.
Millions of rural and urban households use liquidators / liquid vaporizers in routine to ward off mosquitoes. Its efficacy is questionable in many circumstances. Mosquitoes which are knocked down may revive if the concentration of a.i used depletes in time. Does this phenomenon contribute/trigger development of resistance?
I got two photos from two different links. Although both are saying that the species is H. longicornis but the images are not same. Please let me know which one is correct and if it is available is South-east Asia, India or Bangladesh.
Here are the two links: http://www.lowchensaustralia.com/pests/paralysis-tick/haemaphysalis-longicornis-description.htm
Thank you very much.
I need to determine the content of pederin in the brazilian species of Paederus rove beetles (Coleoptera, Staphylinidae). What the best method? Can I estimate the content analysis in lesioned experimental animals or should I use a high performance thin layer chromatography (HPTLC) as other researchers do? The first method is more feasible for me. I can estimate the content of pederin for each species based on the characteristics of the lesions. Is this okay?
why do certain species of mosquito has affinity towards particular parasite or virus? Why don't anopheles mosquito transmit viral diseases?
We are rearing Diamondback moth, Plutella xylostella under the laboratory conditions on cabbage leaves. We are continuously rearing using the same method. However, in one of our rearing cage there is death of almost all the larvae and before death, they became black. Kindly suggest the possible causing agent.
Friends, I am from coastal Andhra Pradesh, India and would like to have some plants having both mosquito repellant and aesthetic significance. Also if possible their availablity in Coastal Andhra Pradesh.
Also can somebody detail in how to identify Chrysanthemum cinerariifolium and Chrysanthemum coccineum plants in a nursery....
Thank you very much
Experimental evidences suggests that HIV infection is not transmitted via arthropods including mosquitoes. However, one paper investigated possible transmission of HIV-1 by African soft tick, ornithodoros moubata. This vector has the potentiality for mechanical transmission of HIV viruses (which otherwise do not survive in arthropods) under field conditions. But this is an old research and any new insights on this topic?
Is this a cause for alarm? As far as we know, HIV is not transmitted by bites of ticks, mites or mosquitoes and there are no evidences to suggest that retroviruses are transmitted by arthropods.
Evaluation of mechanical transmission of HIV by the African soft tick, Ornithodoros moubata.
I found some larvae on my Culex oviposition traps and observed them to be predatory to other mosquito larvae as well as cannibalistic to other of its instars. The larva is slightly larger than of common mosquitoes but smaller than Toxorhynchites. Could not produce a clearer pic as of the moment. Thank you!
I would like to compare the morphometric features of these three forms for any possible diagnostic features.
I will be happy if you could send me fultext articles with detailed methodology and discussion
Hello, I am planning to carry out an experiment involving Plasmodium falciparum infected Anopheles mosquito. For this, I have to artificially infect the mosquito with human blood containing gametocytes, after collecting the blood in to heparinized tube. So, in what condition should i keep the sample to prevent exflagellation of the microgametes for successful infection of the mosquitoes and for how long can I keep it before carrying out the experimental infection?
Insect oviposition is a complex but critical activity in the life cycle of an insect. With a variety of factors that influence both physiology & subsequent behavior, that lead to egg deposition by an insect which tries to ensure safety to their progeny. Many females succeed but few may fail in their attempt to start new life.
I look forward to your valuable inputs (with respect to your model insect & expertise) on how these two key elements could be better utilized for pest control.
You may restrict your answers on the above aspects that are influenced by oviposition attractants & repellents.
Thanking you all.
For the study of mosquito life cycle i needed to know the mosquito breeding methods which are feasible at our area which is dry and sub temperate.....
A similar pattern in Brazil, but with better dengue data. See http://onlinelibrary.wiley.com/doi/10.1111/tmi.12227/abstract?deniedAccessCustomisedMessage=&userIsAuthenticated=false
Recent field tests with human-derived odorants for mosquito attractants to traps did not include consideration of L-lactic acid as part of the human-produced odor blend plus CO2 (Acree et al., Science, 161:1346-1347,1968). Since lactic acid does not elute from a GC column, conventional GC-MS does not see or record it.
Is there a better direct analytical method?