Medicago - Science topic

How much time does the spores needs to establish symbiosis in natural conditions ?

Lets asume, I put a Medicago seed in a 5 ml box, fill with sterile sand or vermiculite, pour some water drops, add 50 or 100 spores and wait the spore to germinate and establis a simbiosis with the seedling. The medicago seed wil germinate in 24 hrs, but what about the spores and establishment of symbiosis ?

How much time should I wait to have 50 % of my seedlings get mycorrhized. Of course, depends of the plant, the AMF species etc, but more or less ? 10 days ? 30 days ? 60 days ?

In our two -year field experiment, white clover pure stand resulted in higher biomass yield compared to its respective mixtures with grasses and as well as with the mixture of red clover with grasses. Although other legumes such as alfalfa and red clover pure stands resulted in lower biomass yield compared to their respective mixtures. However, overall alfalfa and red clover mixtures were more productive than that of mixture of white clover. What are the possible reasons for white clover to produce more biomass yield in pure stand but when it mixed with grasses its mixture produce lower biomass yield compared to the mixtures of alfalfa with grasses or red clover with grasses.

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by self-fertilization. If know anyone how to do. Could you please let me know?

best regards

I am carrying out fatty acid analysis on 3 different pasture mixes. I have a 6 species mix, a 2 species mix and I have divided a portion of the 6 species mix into its individual constituents. In total I have perennial ryegrass, timothy, white clover, red clover, chicory, plantain, 6 species mix and 2 species mix, meaning I have 8 samples in total. I want to look at the differences between the fatty acid profile of the species and then how this changes over time. Initially I thought I would be able to perform ANOVA to analyse the differences between the fatty acid contents of the species, however, the sample prep involved pooling pasture samples from 3 different fields which means the triplicate samples I prepared are from the pooled mix rather than 3 separate samples, therefore I don't think I can perform this. If anyone has any suggestions it would be greatly appreciated.

I am using the A. rhizogenes mediated hairy root transformation protocol. I have tried three recent protocol but the composite plants are continuously producing non-transgenic and chimeric root. As I understand every single hairy root is an independent event but still interested to know how I can get rid of the chimeric and non-transgenic root without chopping under fluorescence adapter. I tried Kanamycin selection (concentration up to 100) but it's not working properly in soybean as worked in Medicago. I have EGFP for screening which working efficiently but I want to have only transgenic root and nodule in the composite plant before the experiment. Looking for ideas and suggestions. Thanks in advance. Cheers, Kamal

I am working with nematode and host Medicago. I have transcriptome data for both species and I also have a list of differentially expressed genes(DEG). Now I would like to see if DEG of host and nematode are associated by calculating a correlation between genes. Which package should I use? I am new in this area so please give me suggestions on network analysis, steps, packages, papers. Thank you!

It is difficult to work on crops and chances of successful experiments are very less, that is why we prefer these model organisms to study the mechanism and then apply the same knowledge on other crops. So, according to you, for different traits and developmental studies which is better?

We have moved the horses of Paddock Sanctuary to different land over the last 4 years, dependent on the forage provided altered if the horses had mild laminitis, ie blood in the white line, and hoof heat. This current land we rent, is high in rye grasses, clovers, buttercups, chickweed, plantain ribwort, nettles. We have found by restricting the horses onto track systems and minimal grass, but ad lib non rye-grass hay forage, laminitic episodes reduced.

It will be interesting to see if you have included pasture findings as part of owner identifying issues.

Dear researchers

Hi

I am isolating a gene from licorice ( Glycirrhiza glabra). the gene is belong to Medicago and involved in biosynthesis pathway. I designed 3 pairs primers. a primer based on the first and the end of Medicago gene. a primer based on consensus regions. I have had a band 3000bp that amplified to DNA of Medicago and Licorice and a band 500 bp to cDNA Licorice. but now It does not amplify 3000 bp band and I sent 500 bp band for sequencing and but Company told me that you do not have any band. I will send my gel picture for you.

please guide me.

best regards

I worked on the globulins of Medicago annual I made the separation by 1-D SDS-PAGE in danaturate condition and I want to recover band from the gel obtained.

If you have for instance 100 markers and all are mapped in the same linkage group. These markers have a physical positon on chromosomes. So, I found that these markers have positons on different chromosome of Medicago truncatula... .

what do you think?????

I want to use the selection medium with kanamycin (100 µg/ml) as selection. Can I use this concentration? Or is this concentration above the alfalfa lethal threshold?

Thanks

I did association analysis using SNP for some traits in medicago. As a result, I got some significant SNP associated with traits e,g Vf_Mt2g086880 . These SNP generated from Medicago. So, I would like to do synteny to gene function.

Dose anybody know a way to compare my SNPs with those already their function?

I want to collect seeds from various farms within the agro-pastoral region of the Western Cape Province of South Africa to determine the quality of the soil seed bank on each of the farms and how different pasture/crop rotation strategies influences not only the quantity of seeds in the soil, but also their vertical distribution within soils. I was also considering trying to determine the natural breakdown of seed coat dormancy on each of the farms by collecting soil samples at various stages after seed formation in order to try and get a rough timeline as to how long this process will take. However, I am not sure how I should go about testing the 'hardness' of the seeds. Would normal petri dish germination trials with the collected seeds be enough to test the ability of seeds to germinate at various time intervals after seed formation?

Rhizobial inoculation of annual Medicago and Trifolium species are suggested by seed distributors. This is primarily to help with nitrogen fixation, however would these bacteria also help with seed germination and subsequently seedling emergence from deeper burial depths?