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Marker Assisted Selection - Science method
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Questions related to Marker Assisted Selection
Molecular markers are specific DNA sequences that can be used to identify and track particular genes or genetic traits of interest in plant breeding programs. These markers serve as tools to assist breeders in selecting plants with desired traits more efficiently and accurately. Molecular markers can be used to determine genetic relatedness, assess genetic diversity within populations, identify specific genes responsible for traits, and facilitate marker-assisted selection (MAS) or marker-assisted breeding (MAB) techniques.
Could you please recommend some molecular markers for identifying the genes for disease resistance such as blast (Pita, Ph-k, Piz), bacterial leaf blight (Xa12, Xa7, xa5, etc...), brown plant hopper (BPH17, BPH18, BPH37, etc,...). Thanks!
I run my gene specific primer PCR product on 2.5% Agarose it did not show any amplification only primer dimer was seen
But the same product I used in 8% PAGE gel (with silver staining process) it shown the amplification result
What is the reason behind it...


Should i recalculate the concentration of the protein after adding loading buffer into the sample, so i could calculate the exact volume sample i need to add into the wells to make sure the amount of total protein is the same. And another question if i have added the same amount protein of different sample, the internal marker(eg:GADPH) is not consistent, what i should do as the mathematical calculation is not working anymore. Thanks.
Hello to everyone,
I am looking for an overexpressed marker (intra or extra cellular) in the PANC1 cell line for flow cytometry analysis. Indeed I realize coculture with cancer cells and CAF (cancer associated fibroblast), at the end of the coculture I analyze the ratio beetween cancer cell and CAF by marking the cancer cells with an overexpressed surface marker like Epcam to know the proportion of CAF and cancer cells.
I try to do this experiment with the PANC1 cell line but I have some difficulties to find the right marker because Epcam is not expressed enough to distinguish CAF and PANC1.
I already tried Epcam, EGFR, Cytokerin 7 and 8 but I can't distinguish the 2 populations .
PS1 : CAFs don't really have a specific surface marker either as far as I know ... except aSMA but it is not strong enough to distinguish the 2 populations
PS2 : I can't mark the cells with CFSE type dyes because I let my cells in culture that proliferate and it's not great with this kind of markers.
Thanking you for your help
Hi,
I'm new here so sorry If I'm in the wrong place.
I've been working in UK Agriculture for around 10 years now and I've decided to pursue a career in R&D and plant breeding after working for a seed production company with a local breeding site. I found it all fascinating so I've started a biology degree at Open University and I'm hoping to get a job as a technician or something similar soon.
I know I'm still a fair way from my ultimate goals but in the mean time I'm looking for any good books that go into detail on agricultural plant breeding methods, plant biology, genetics and modern techniques such as Marker Assisted Selection, Doubled Haploid and tissue culture.
I'm looking for something that covers these subjects in detail but on a suitable level for a student (I like diagrams and pictures haha). One of the best looking ones I've found so far is "Plant Biotechnology and Genetics: Principles, Techniques, and Applications" by C. Neal Stewart Jr. But i could do with some advice from anyone who's read any books like this in the past.
Thanks
Stuart
Turmeric is a polyploid and vegetatively propagated crop. Usually, F1 populations are used to identify QTLs. As this crop generally has no viable seed, what is the best approach to genarate QTLs.
If Mesenchymal Stromal Cells (MSCs) are used in cell therapy, what are the stromal cells and in what proportion they are recommended for cell therapy? I appreciate the guidance, reference, suggestions or recommendations.
Or if Mesenchymal stem cells are used in cell therapy, how to differentiate Mesenchymal stem cells from stromal cells? Is there any specific marker to differentiate them?
Thanks for your feedback.
I want to find up-to-date information on the current state of conventional (traditional) plant breeding in the world. Is the conventional breeding approach applied in the small breeding companies or crop giants like Monsanto and Syngenta now? For example in order to produce lines for subsequent marker-assisted selection (MAS) or genetic modification (GM). Or the traditional breeding approaches are currently totally substituted by the MAS and GM approaches?
If you know reviews and/or book chapters on this topic please suggest.
Suppose I have scored 6 allele for an SSR marker in 50 population with major allele frequency of 0.2448. Now how can I determine the required PIC for the marker?
Hi everybody. I'm wondering weather or not carboxymethylcellulose can be used and metabolized as the only carbon source in a medium for a microorganism able to produce an endocellulase. Specifically, I'm trying to use this endocellulase gene as a selection marker (no antibiotics, no auxotrophy marker). Is it possible to do that? Will the carboxymethyl glucose generated by the enzymatic reaction be metabolized by the microorganism?
If the experience is to find out marker assisted selection/MAS, what kind of strategy (or what kind of molecular mark) that can be best to be used?
I found several strategy lik RFLP, SSR, AFLP,, QAPD, QTL and SNP for several kind of selection for drought tolerance plant. If this research is conducted in rice plant, what kind of molecular mark is suitable for that?
Would you please contribute your opinion
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I fed in my diploid data (29 samples, 18 primers (loci) and average of 8 bands/locus) for dominat analysis for Shanon Index, total allele diversity, percentage polymorphism etc sucessful. But at the last comment of analysis, the dialog box showing OUT MEMORY was displayed. How do i overcome this problem?
microsatellite markers used as marker assisted selection (MAS) in wheat, rice or barley for commercial breeding program
which is the perfect allele size
Do you know this one? So, Please let me know the reason.
Recently I tried to do test the possibility of using the geneticin in A. fumigatus in the 400 ug/ml concentration. But it is not working. I am trying to figure out the reasons. Help me please
Fusarium head blight, powdery mildew, wheat yellow mosaic bymovirus, and sharp eyespot are important diseases of wheat in the middle and lower reaches of Yangzi River. Pyramiding resistances to these diseases is not an easy work.
Replies from respected fellows will highly be appreciated
Hi everybody
I have some specific markers for drought tolerance in wheat. Can I use the same marker for drought tolerance in barley? In other words, if I run some experiment to test drought tolerance in some barley genotypes and then I use the specify maker for drought tolerance (in wheat) to genotype the barley lines. If I found a significant association between marker and drought tolerant trait in barley, is it possible to say like a qtl?
Hi everyone. can you help me with a procedure for marker assisted selection. A website link or PDFs where l can find the answer will be greatly appreciated. THANK YOU IN ADVANCE
I have found following summary last week, but unfortunately I have not saved the link:
2. Main objectives of the project – Project summary
The 21st century is a time of continuing population growth. If we are to shape and profit from plant breeding, for the mutual benefit of the consumer and of industry, we must integrate innovative methods for generating variability and fixing genotypes. The use of doubled haploids, particularly in combination with marker-assisted selection, has sped up transgenic and non- ransgenic breeding programmes and broadened the gene pool from which attractive traits are selected. Despite the well-established advantages of doubled haploids and their enormous benefit for breeding, they are still difficult to obtain in many important crop species. As a result, an enormous and costly effort needs to be invested by industry if they are to be universally employed. Here we identify crucial bottlenecks to the development of doubled-haploid technology and propose a work-plan for their removal. Based on our preliminary results, we postulate that epigenomic regulation is a crucial regulator of plant cell
Reprogramming and hence totipotency. Epigenetics defines a set of mechanisms mostly consisting of chromatin alterations that modulate DNA transcription, dictate tissue and cellular complexity and bring the phenotype into being. Chemical probes targeting key enzymes of epigenetic regulation are likely to be crucial to manipulate the plant cell epigenome and shift differentiation processes from the default pathway towards embryo formation.
I intend to study the characterization of indigenous chickens in Zimbabwe using molecular markers. Nothing of that sort has been attempted and I need collaborators. Please contact me if you are interested
The primers we are using are also the ones validated in several studies and PCR condition is very specific (touchdown). Thanks for your time.
Hi, I have always been using synaptophysin as a presynaptic marker, unfortunately as other presynaptic markers its penetration in the tissue is very low, usually less than 3 or 4 microns when using with 0.2% triton. Does anyone know of a synaptic vesicle marker with better penetration?
My understanding is you plant many varieties in the field or greenhouse. You observe and measure for your desired traits. You genotype your entire population. This is where I am stuck.
How does phenotype observations + genotype sequences = molecular markers that we can one day use for marker assisted selection (MAS)?
How do I find molecular markers for desirable traits such as rhizomes, non shattering, and large seed size in wheat and sunflower?
I want to search markers for MAS screening of Xa-10 gene of BLB resistant population, does anybody have marker for the same?
I want to generate a marker for the RF gene.
Can anyone succinctly explain how one differs from the otherand why a breeder would use one over the other?
In genomic selection, the model estimates the substitution effect of each SNP, which is the difference between the average effect of its two alleles. GEBV is then obtained as the sum of the substitution effects over all SNPs.
Is it really the same thing as the breeding value, which is the sum of the average effect of all the alleles?
In many crops the genomes have sequenced and plenty of markers are available for most of the important traits. Is it possible to develop a high yielding variety having all desirable quality traits coupled with resistance/tolerance to important biotic and abiotic stresses?