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Marine Biogeochemistry - Science topic

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How can I convert plankton concentration (micro, nano, pico) from mg/m3 into mol/m3
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@Kunal Madkaiker I see, that information was missing from your question. To do that, you could start with this one: Behrenfeld, M.J.; Boss, E.; Siegel, D.A.; Shea, D.M. Carbon-based ocean productivity and phytoplankton physiology from space. Glob. Biogeochem. Cycles 2005, 19.
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I think it's an interesting question: both physical ocean and marine biogeochemistry are involved, and perhaps enlightening for marine ecosystems.
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@Fanyi Meng The book 'The Extreme Life of the Sea' by Anthony R. Palumbi and Stephen Palumbi might help you.
This article might also be helpful
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I want to know which is the best part to look at to compare if coral calcification is affected by in situ low pH environment. I've been reading papers but I can't pinpoint which part they are looking at under SEM. My fragments are from the genus Porites, Goniopora, and Platygyra.
#corals #calcification #microstructures #skeleton
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Hi Joana,
I am not 100% sure what questions you are asking but if you are interested to compare the effects of ocean acidification (i.e., lower pH) on coral skeletons you have some options. Looking and comparing basic skeletal corallite structures at macro-morphological (e.g., corallite diameter and wall thickness; septal and costoseptal thickness and high) and micro-morphological levels (e.g., septal teeth height) would be relatively easy. You can look at the thickness and porosity of the skeletal material in general. Recently new studies have been done on pH effects on crystallographic features of coral skeletons (see attached paper). I hope the attached papers will assist you in getting your answer, the reference sections are full of good papers.
Cheers,
Tom
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Suaeda maritima.
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Yes. Please see the following RG link.
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We are identifying a large numbers of detrital mineral samples from the marine sediments. We have excluded staining method because it takes a lot of time.
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For microscopy, we should not forget that determination of refractive indices (RI) can be quite useful and fairly quick. For example, if you use a liquid of RI 1.527, Or grains would have lower RI in any orientation, whereas Qtz and Pl grains would have highern RIs. Carbontes should not pose any problem because of their high interference colour. But the procedure is tedious and gone unpopular.
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Most of devices are set for measurement in range of salinities from fresh to ocean. But in the world exist many lakes and other water bodies with extra-high salinity.
Who knows about the latest advances in device measurement of Dissolved Oxygen in highly saline waters and brines?
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Dear Peri, I plan to publish it as a book, guide or manual "Determination of nutrients. Application for higly saline waters". It would be great to complete this work by the next ISSLR conference in 2020.
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What are the new approaches and research directions on ocean deoxygenation and its effects in benthic communities of coastal marine systems?
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I have often wondered about the extent of damage caused by the common practice of dumping urban sewage wastewater into the ocean..
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To what extent biomass burning (or forest fire) affects marine productivity?
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Adverse impact
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The isotopic composition of organic tissue of a specie of deep-water coral will be considered to evaluate the efficiency of food uptake under controlled conditions in a microcosm.
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Hi Renato. Did you try to use ultrasound bath?
We put coral pieces in eppendorfs with buffer or milliQ water, and put it in an ultrasound bath for some minutes to separe tissue from skeleton.
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Hello,
does somebody know to suggest me a reference study estimating/measuring the general relationship between PON (particulate organic nitrogen) and chlorophyll-a in marine phytoplankton? Even better, specifically for the groups of marine diatoms and cyanobacteria?
Thank you in advance,
Ioannis
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Thank you very much for the references, very helpful.
Best regards, Ioannis
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When there are anoxic conditions in sea, due to microbial respiration H2S is produced. How can we measure that and what are its impacts on marine ecology?
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You can e.g. measure sediment H2S concentrations and dynamics via H2S microsensors (available at Unisense A/S). Hydrogen sulphide is generally considered toxic to animals and plants.
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These are formations found in the pump house of a salt work near Port Elizabeth, South Africa. The amber-like yellowish-brown substance inside the formation is hard at the edges but still soft in the middle. What is it and what is likely to produce it?
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I've also been trying to figure out what the formations are. Adding onto the possible cyanobacteria answer, Nostoc pruniforme heterocysts (Mare's eggs) are formed in fresh water environments but there may be similar nitrogen fixing cyanobacteria formations in saline or hypersaline environments; purely a guess but would be interested to find out.
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I would like to keep the coral samples alive for Zooxanthellae and DNA extraction purposes
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Any Branching corals such as Acropora should be kept in plastic bags half filled with seawater and oxygen. The coral pieces should be fixed in thermocool"" and each piece of coral should not touch each other. Same way other Montiopora digitata collected in plastic bags should be kept in thermocool rectangular  containers with some icecubes for reduction of temperature. care should be taken that the coral pieces should not secrete a large amount of mucus. These corals may withstand 24 hours transportation through air cargo.
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Can anyone suggest the best method to do Fe-Speciation in the surface sediment..?
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As i asked above, is it necessary for us to differentiate the two kinds of alteration caused by hydrothermal and sea water, respectivley?  Any one can give me some suggestion, please.
I've already found what i need, though the question is somehow a liitle unclear.
ANYWAY THANK YOU GUYS.
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Perhaps "weathering" is what is meant?
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This question came up after reading the paper by Engelhardt et al., 2015.
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So it seems that C N and P from viruses could support benthic communities based on the work by Dell'Anno et al 2015.
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I am willing to do some researches about the different kinds of alterations in the basalt in the ocean. And I want to know if there are some books or articles describing the changes of REE, isotope or major,trace elements during the alteration processes
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Dear Cheng Yang,
For dealing with REE studies in basalt and so on basically please refer the books wrote by Paul Henderson as the title attached below:
REE geochemistry (1984) and In organic geochemistry (1982).
Best regard's
Zolhizir bin Daud.
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Sedimentation? how long the hard coral exposure? and its will be killed by sediment?
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The USGS has done studies in HI that may help you address this question - I suggest that you review the publications at http://coralreefs.wr.usgs.gov/pubs.html.
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I need the earliest data available.
Thanks
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concentration in seawater
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Ocean biogeochemical models still have  a very crude parameterization of bacterial dynamics. Some models consider a mechanistic degradation of particulate organic matter depending on its quality (nutrient content) by free-living bacteria, and other models have an implicit linear or non-linear degradation, which implies that all bacteria are attached to particles. As far as I know nobody has considered both attached and free-living bacteria but I may be wrong
thanks in advance
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Hi all
thanks for your answers.  It is indeed an interesting scientific question and it looks like it would deserve some more attention. I do agree with Kenneth that almost all bacteria are attached to something, being it dissolved or particulate, however if we consider the trophic role of bacteria for zooplankton (HNAN), it is likely that this occurs mostly on the free-living portion. Indeed, experiments do report that adding particulate organic matter to axenic zooplankton incubation does not lead to much biomass increase.
I think that one major issue of being attached to particle is that they actually sink with the organic material and the degradation goes on while the particle is settling. This is actually what is parameterized in the models that resolve bacteria in an implicit way. 
Thanks Charlotte, I'll have a look at the paper and see if it gives some more insights
marcello
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The polyphenols in the sheath material (the link between rhizome and leaf) is, contrary to that in the leaves (which end up in the banquettes), composed of non-lignin phenolic moieties with very little methoxylic groups. This seems to be the section of the plant, besides roots, that accumulate in P. oceanica seagrass mats at the sea bottom. Does anyone know more of the characteristics of these phenols. Could it correspond to something like "neolignan"?
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Thanks Jai. The thing is that these phenols have no methoxylic groups, they are based primary on the p-hydroxybenzoic acid moiety.
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We are trying to see the effect of sedimentation on wildlife in Tanzania and we can't seem to find any effective but yet inexpensive way of measuring sedimentation. any advice would be much appreciated. 
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We have done some experiments to understand the sedimentation process in our seaweed cultivation farm. We have used small glass bottles with round bottom and placed them for 24 hr in  the field. The samples were collected and data was expressed over area of the bottle. The only precaution which you need to take is the bottles remained in the same plain as the topography of the sea bed.
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I am wanting to get some empirical measure of sand transport patterns, directions and volumes into, within and out of a particular beach embayment, and am wondering what is the current state of the art in this area? (e.g., tracer particles, high-resolution bathymetry for interpretation of bedforms indicative of currents transporting sand, etc?)
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Chris, Attached is the pdf.
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Barium in marine sediments controlled by terrigenous supply and diatom production. But what controls Gallium in marine sediments? Is it only by terrigenous?
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Dear Mr. Balaji,
barium is primarily controlled in marine environments by magmatic processes, being, in places, instrumental for the emplacement of Kuroko-type (VMS) and Rammelsbergy-type (SMS) base metal mineralization, dependant upon the geodynamic setting. It goes  along with the precipiation of baryte. Ba can substitute for K in all K-bearing silicates and as such increases along with the detrital input into the basin. Gallium can replace to some degree aluminium in its host minerals and as such will also act as a marker of the detrital input.
Best regards
H.G.Dill
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Cold water (20°C) extractable organic carbon (CWEC) and Hot water (80°C) extractable organic carbon (HWEC) at the bottom of lowland peat (Haplic Histosol) and Gleyic Histic Fluvisol on turf (70-80 cm)? See Figure in File. Pay attention to the groundwater level !!!
Thank you.
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Assuming no direct anthropogenic intervention,
I would think in terms
  1. The global warming phenomenon, degradation of surface soil organic matter is increasing yielding lower molecular weight and water soluble metabolites. The increasing rate of release (temperature being the driving element of microbial activities- degradation, which promote the leaching of the organics to deeper layers where it accumulates due to the restrictive effect of the ground water table.
  2. The release of fertilizers such as nitrate and phosphate into water bodies can also impact of organics transformation and their ultimate behaviour (chemical and or physical) characteristics in the soil profile.
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Hello everyone,
Have any of you used CT scans of sediments to get a density profile/trace? Specifically, I am working with marine and lake sediment cores. I have used CT scans before for their imagery and have exported profiles of their densities based on their grayscale values in ImageJ, Sante DICOM Viewer, and Osirix, but we'd like to move into using our CT scans a bit more quantitatively. I processed the dicom files with the same window and level settings within individual cores, but was not always able to use the same settings from one core to another when I created images (.jpegs, .tiffs, etc.) from the dicoms (sandier cores would be washed out or muddier cores would be too dark to see detail otherwise). Now, though, we'd like to access the actual, or as close to actual, densities as possible. I've read a little bit about calibrating CTs to a known value (I was thinking air that got imaged around the cores, or their PVC/plastic core liners) and about using the Hounslow scale to at least approximate actual densities. I was wondering if a) anyone's got any thoughts on finding good ways to tie CTs to actual densities, and b) if anyone can think of any pitfalls to watch out for while I do this.
Thank you for any help or suggestions you can give!
- Bran
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Hello Mihai and Ferdinand,
Thank you both for responding so quickly! Mihai - looks like you've confirmed some of my concerns about properly calibrating my scale. I think it's going to take some special consideration. Thank you for the link, Ferdinand. I will take a look. 
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Please help me  know this
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Indeed, those are extended tentacles. The pink indicates damage and that regeneration (growth) is going on. Species: Fungia fungites.
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In Ennore creek in southern India I found BCF (Bio concentration factor) of more than 2500 to 3000 in crustacean crab and polycheates. Will an estuary which is highly polluted resume this much BCF? Please help me to solve this BCF for Nickel metal.
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Sharon, Thank you for sharing answer
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The question revolves around the concept of acidification of estuaries, as an ecological process which may be induced by human activities. In tropical conditions, high rainfall, heat and high primary production and specific geological settings may determine soil acidic conditions, which may control relatively low pH conditions in estuaries (i.e., in the lower tract of hydrogeographic basins). I am looking for the available literature and an overview/discussion on the state of art of this topic.
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Hi Gianluca,
I cannot provide a straightforward answer, but I will make a suggestion nonetheless. Please have a look at these papers. probably you can find some useful info there:
Cheers,
Marcos
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I am correlating pCO2 with fluorescence in the Western basin of the Mediterranean Sea. This basin is oligotrophic, but in the spring blooms can occur. I was expecting during this season the pCO2 to decrease due to the biological activity, but the correlation coefficient is positive. What could be the cause behind that?
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I do not agree with what Alastair has said, pCO2 depends on pH of the water, it is other way around, pH of the sea water is dependent on pCO2. moreover, blooms can be also limited by CO2 in the oceans if not having constitute CCM. an increase in pH has been observed many times with enhancement in phytoplankton biomass. However, I agree with the last part of your answer. and also what Koji has mentioned. This is possible that for warming or upwelling, significant amount of CO2 is added up to the surface water and there could be simultaneous uptake also by phytoplankton and enhancement of fluorescence. However, the net increase in CO2 could be more and hence positively correlating with the fluorescence. In the Oligotrophic condition under high solar light there can be photo-inhibition leading to net heterotrophy and can may not uptake considerable amount of pCO2. As Koji said, phytoplankton stock is also important.
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The overarching goal of this study is the rehabilitation of the salt marsh, which is funded by introducing a selected range of tourism opportunities (nature, scientific and cultural tourism).
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Hi Mark, in a recently research we conducted on wetlands in Colombia, which was based on expert knowledge and participative mapping, we found that salt marshes are especially important for regulating services, followed in importance by cultural and provisioning ES categories. Likewise, we assessed the importance of the ecosystem functions and our findings showed for salt marshes are important for regulation, followed by information, production and habitat functions.
If you need to think about economic activities for restoration plans of salt marshes, I would definitely recommend including the cultural services for example, tourism, fauna observation, scientific trips and scholar activities, but would suggest keeping the local community involved in it. As the local community has an economic benefit, people will keep the marshes in the best state as possible. How well you know the plants of the marshes? I am sure that there are some possibilities for handmade products… that is very common in the Tropics. Other activity you could explore is fishing (sport and commercial), which might be a key livelihood in the region. The central point here is the biodiversity. 
Cheers!
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Found in Hudson River Estuary in October, salinity ~10 PSU
Size of individual segments is about 800 - 1000 um.
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as Clifton said, possibly polychaete branchi, or feeding tentacles if there is a groove on one side.
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If anybody knows the analytic procedure for the extraction of free sugar or mono saccharides from sediments or soil ? If anybody have standardized any method to quantify total sugar present ?Any one have any reference paper on this?  How to get the sugar analysis procedure by HPLC RI detector?  
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Thanks Arun Pandian.. If any method available other than colorimetry..?
HPLC RI detection of sugars.? 
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Measurements of dissolved gases (oxygen, CFCs,..) in sea water samples is usually done on unfiltered samples, and tedious protocols need to be followed to avoid any bias (must be first sample to be taken, quickly after collection, no bubbles, overflow bottles 3x volume,...).  DGM measurements are done on both, filtered and unfiltered samples. Could filtration result in any loss of Hg(0) or dimethylmercury?
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My opinion is that water filtration will result in loss of dissolved gasses as filtering is connected to change of pressure, especially if vacuum is applied. Certainly filtering have influence on dissolved Hg gasses (Hg(0) and DMeHg). It should be checked in filtered and unfiltered water immediately after sampling.
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I need a good idea about the analysis PAHs in sediment for PhD thesis. There is a 120 sediment samples of Persian Gulf. They are offshore samples. I wonder what is the best that I could run with this samples as a PhD thesis. I know somehow about GIS and RS. Any idea about joining the laboratory analysis with this technique? We don't have any samples of fauna.
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there is a lot of papers for sediment preparation for GC/MS measurement.
just Google it and you will find many.
but the important is to find good internal standard and start your tests.
its not hard but you need to practice it.
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when sampling the marine organisms such as crabs from along the mangroves they can be preserved let say in ethanol while they have some sediments. So if one need to extract DNA from part of these samples can't these sediments influences the quality of DNA extracted and therefore possible to affect the PCR products?
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Dear Nehemia,
is always better to clean your sample (with a preservative rather  than with water to fix the DNA in the tissue, and to avoid DNA damage) prior long-term storage. Not cleaning your sample will induce false results, as bacteria and other microorganisms (algae, ...)  living in the sediments could be co-extracted with your sample giving you mixed results. ie. your PCR product if done with universal primers will represent the "ecosystem" that you have extracted (crab+sediments), don't allowing you to assign the resulting sequence to your target organism (crab), well, you can but, you will not be able to give clear results.
Some preservatives are acetone, ethanol (96% rather than 70% for long term storage, as the water of your sample will dilute the ethanol),  or formaline. For marine specimens is commonly used Formaline with formaldehyde and sodium bicarbonate.  But I'm not too sure about the last one in preservation of undamaged DNA.
hope this helps you
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Now I only have the discrete wave parameters, such as wave height, wave period and wave direction. How can I use these parameters to calculate the sediment transport rate caused by wave actions on the tidal flats, an extreme-shallow water boundary with small bed elevation gradient and large width of tidal flats ? If I do not consider the wave breaking, is there any simplified formula or model can be used to calculate the sediment transport rate ?
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Walton & Bruno's equation considers breaking wave and hence might not be suitable for the case of large tidal flats with very low gradient. I would consider the sediment transport over large tidal flats with low gradients to be as flow induced sediment transport using pure current generated by the wave at the nearshore boundary similar to swash flow induced sediment transport.
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Here we have only calculated the impact of diapycnal fluxes on the sea surface pCO2 - however significant entrainment of deep water into the surface mixed layer will also impact on the sea surface value of pCO2. Given this change will essentially be a "shock" when compared to the much slower changes due to diapycnal fluxes - has anyone considered the relative timescales of the growth (ie. DIC/ NO3 utilisation) and equilibration on pCO2 - and whether it is even important when compared to the slow diffusion of CO2 across the sea surface?
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Hi,
if you haven't read it already, you might find this paper (and references therein) relevant to the subject:
Sallee et al., 2012. Localized subduction of anthropogenic carbon
dioxide in the Southern Hemisphere oceans. Nature Geoscience, DOI: 10.1038/NGEO1523
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In view of the multifarious analytical procedures involved in examining elemental isotopes (C, N, S, O) in different samples (sediments and water), I would like to know if there is any standard procedure (also include sample storage/preservation techniques and sample pretreatments) to measure stable isotopes in sediments and water samples. Also, it would be extremely helpful if any experts here are able to share with me some information on how to analyze C13 in organic and inorganic phase using an EA-IRMS. Your generous input is very much appreciated.
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Here is a solution practises (has a practice), if you have only the coupling EA-IRMS, you can proceed in this way:
- On a sample of homogeneous sediment you measure global 13C (carbonate + organic matters)
- On one other fraction acidified with the HCl with pH 2 and washed well and dried to measure you again the organic 13C.
From the intensities of peaks CO2, to calculate you the quantity of the CACO3 in your sediments.
So to calculate you the values of the 13C of carbonates.
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I'm thinking on buying this digital microscope (examples in the link) for foraminifera picking in wet sediment samples. Have somebody tried them for this exactly purpose? I know they are excelent for taking photos, but could they replace the good ol' stereoscope?
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Hi Jorge,
I would like to add a quick comment on this too;
I've been using one of these usb microscopes, but a cheaper version (from ebay), on my shale cores. Since my cores are dark in colour, I've been having hard time to get good results (led lights do not work very well on my cores). However, when I look at a smooth surface, such as a mouse pad, it works very well. Moreover, when you are looking for forams, if you would like to hold it, instead of attaching it to its stand, it is definitely not going to work; you won't be able to focus properly.
So, my suggestion is that try a cheaper version first, you can find one from ebay (~35 $). If it works go for it, because it is portable and easy to use, plus you can take pictures (I assume up to 2 MP; don't expect a regular camera quality). If not, you will save some money, and you might use it for other purposes, such as showing hand specimens on a projector to students...
I hope it helps
Cheers
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Although I have been able to get most of the information related to Landsat 8 and its bands from landsat.usgs.gov website, still, I haven’t been able to explore the complete potential of its bands, especially the new Band 1 (Coastal/Aerosol Band : 433-453 nm) and its application in coastal and near shore processes research. I tried searching for previous research articles in using coastal band but I was not quite satisfied with the search results.
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Dear Dr. Hubert, this is an excellent research paper link which you have provided by Vanhellemont & Ruddick. Precisely what I have been looking for. I have started following your updates. I see that you have also done some work in 'ocean color remote sensing'. Are you still associated with the coastal water study in Hanoi? Thank you so much for your pertinent answer. 
Dear Dr. Mauricio, you have a very keen sense of observation. This is a brilliant idea. Thank you for this first-rate research paper. Those guys in Scripps have been doing some great quality research. I am deeply thankful for your efforts in putting together these significant research papers related to S.California bight and Chile's river plumes. Have you also been doing your research in Chile area?
These are some of the finest answers I've received so far for my question. Since I am just a budding researcher my motivation has always come from being able to observe things by 'standing on the shoulders of giants' like you. Therefore I will be indebted if you have any advice, suggestions or recommendations in order for me to do some quality research which would be beneficial for humans and mother nature alike. Right now I'm at the stage of collecting and examining closely relevant research papers related to remote sensing of coastal regions, all the possible threats which might range from small-scale sand erosion to mighty coastal hazards and the prospect of planning mitigation measures along with analyzing pre and post disaster scenarios for coastal resilience. Conservation of biodiversity in these regions is another area of concern for me.
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I have kept attention on the quantification of the contribution of each organic matter source to the coastal salt marshes. In general, carbon and nitrogen radioactive isotopes are used in the mixing model to calculate these contributions. However, these two indexes are not enough to solve the model when the number of the organic matter sources is larger than three, which might include autochthonous source like plant debris, and allochthonous sources as fluvial sediment, off-shore planktons, benthic algae, etc. In that way, C/N ratio can be an index used in mixing model for source partition and contribution calculation ?
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C/N ratios of bulk organic matter are cheap and easy to determine with high accuracy. However, the problem is that the C/N ratio of each and every organic matter source varies greatly, and not just with the tissue type of the source material (there is great differences in the C/N ratio between woody tissues and leaf tissue of the very same tree, for example) but also with the level of organic matter degradation. In a forest, the leaf litter on the ground may have C/N ratios between 20 and 30. In the underlying topsoil the ratios will have dropped to values between 10 and 20. Degraded organic matter in some clay-rich tropical soils (e.g. oxisols) can have C/N ratios down to 8. Litter and soil are already two potential end-members on the terrestrial side with great ranges in their individual C/N ratio. Plants from the littoral zone have variable C/N ratios, bacterial biomass has very low C/N ratios (~4) etc. ...
As you already recognised yourself there is a great range of sources, definitely more than three, and organic matter degradation will just add further variability. It depends on your study site. In some settings, where long-distance transport processes have averaged out the details, C/N ratios may well work to identify, for example, phases of generally higher or lower terrestrial input. However, using C/N ratios to disentangle contributions from anything more specific than the usual aquatic vs. terrestrial organic matter sources, I'm afraid, this won't work. This requires a multi-proxy study and organic-geochemical fingerprinting of the potential organic matter sources in your study area. Isotopes are a good start and biomarkers are very useful, too.
Hope this helps.
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It was found at in an Irish marsh exposed to fresh and marine water (depending on the tide) and was living on top of mud. It is not flat (picture can be deceiving) and is shaped like half a lemon. It is about 2mm long. Looks like some sort of slug, but I have no idea where to start. When alive it moves like a slug across the surface of the mud. The one in the pic is preserved in alcohol. Any help would be very much appreciated!!
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Hi Dannielle,
Apologies for the sluggish response. Looking at the later photos, I would think that these are gorged (just after feeding) Turbellarids (flatworms), likely of the order Rhabdocoela and suborder Kalyptorhynchia. The presence of the eye-spots (I didn't notice these in the initial photo) are what leads me to Turbellaria, the seemingly bulbous structure of the organs and overall body shape leads me to suggest Kalyptorhynchia.
Despite my suggestions, it is always tough to identify organisms from photos, so please take my identification with caution. I would suggest preserving the specimens and sending them off for proper, expert identification. 
Best regards,
Jeff
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chlorophyl-a algorithm for MODIS, SeaWifs
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OC4v4 is the current default algorithm for SeaWiFS, while OC3M is the current default algorithm for MODIS (Aqua).
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Micro-mole per liter?
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In marine research all concentrations are given as a molality, e.g., micro-mols per kg solution. This is important because molality does not change due to a change in temperature, pressure or salinity of seawater. Remember, the volume water changes with temperature and pressure, but the mass stays the same.
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What difference does it make to have too much excess acid?
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Your goal is to get a sensitive measure of the change in hydrogen ion concentration as you add acid. If you are close to the equivalence point in a single point titration, then you can better achieve this. An uncertainty of 0.001 in measuring the final pH is equivalent to an uncertainty of about 0.23% in the hydrogen ion concentration. If the pH is 4.0, this is a significantly smaller absolute number than if it is 3.5.
However, sensitivity is not your only problem! You also need to minimize bias. The largest contribution to bias is in your pH measuring system's calibration, which needs to be done ahead of time for a one-point method.
The next largest contributor to bias is bicarbonate ion that has not reacted. There are a variety of ways to deal with this. The best is to bubble the acidified solution with air for a few minutes so as to remove CO2, thus reducing total carbon, and hence the absolute amount of bicarbonate ion present at any particular pH. A slightly lower pH also reduces the proportion of the total carbon remaining that is present as bicarbonate.
Also, in a one-point method the amount of acid you add needs to ensure you get an OK alkalinity for all the samples you intend to measure. Unless you adjust it with some a priori knowledge as to the true alkalinity, you are bound to have compromises.
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I am trying to find the REE concentration in marine sediment and river sediments. I would like to know what the most suitable spectroscopic technique is? Can we efficiently analyse REE using ICP-OES?
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ICP-MS is definitively better. For sediments, dissolution methodology is also very important. If you want to track paleoenvironmental signal in the labile carbonate phase, you better use a "light" dissolution technique (such as acetic acid) in order to avoid dissolving terrigenous matter (and therefore contaminate the environmental signal).
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I am interested in getting various methods of CO2 measurement in seawater.
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You can use pH and TAlk (Total Alkalinity) to computed DIC (Dissolved inorganic carbon) and then pCO2 (partial pressure of CO2). This indirect method can be easily used in any field sampling condition and it is far less expensive and more easy to set up than the direct one (using the IR analysers). TAlk is measured by Gran electrotitration. pH is measured with a combined electrode. In seawater, the electrode is calibrated on the Total Hydrogen Ion Concentration Scale using the buffers prepared according to Dickson (1993). In contrast, in estuaries and freshwater environments, the electrode is calibrated on the National Bureau of Standards (NBS) (Frankignoulle and Borges, 2001). I particularly use this method to get CO2 data in coastal environments (e.g. lagoons, mangroves, ...) and in rivers.
Note that in practice, the pH of samples is computed using the Nernst equation.
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Sandy intertidal sediments in the North Sea
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Hi Alexandra, I don't know the answer to this offhand, but can recommend a few places for you to look....
These are UK-centric though, and so will only cover the UK's part of the North Sea.
Try the UK Seamap at: http://jncc.defra.gov.uk/page-2117. There is a link on that page to a PDF document called 'substrate', which explains the classification method and may have some useful info for you.
Also, if you can get your hands on a copy of the digital BGS seabed sediment map, then you would be able to calculate sandy sediment coverage for your area of interest.
http://www.bgs.ac.uk/products/offshore/DigSBS250.html. Usually BGS charge for this product, but dependent on what you are using it for, you may be able to get it at a reduced cost. Even if you can't get a copy, it is a good source of info and by searching online for eg 'sandy intertidal sediments North Sea BGS DigSBS250' or similar, you may find a report or paper that has already calculated what you want to know (again this will only cover the UK area of the N Sea).
Also, you could try CEFAS, or other fisheries research agency (ICES for a whole European view). They may have this information somewhere, as these habitats may be important for some fisheries (shellfish etc).
Anyway - I hope that is helpful. Hopefully you will get comments from people working in other North Sea countries, who can recommend other sources of info!
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How well can a freshwater marsh be restored to a saltmarsh by increasing the amount of saltwater entering an impoundment?
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My guess is- quickly if you have the desired species present and can maintain water levels without flood or drought. However, your question is site specific.
Some of the factors you'd need to consider to answer that is the inflow an outflow volumes and salinities, obviously, and their capacity for mixing, which may involve clearing surface water pathways and factor geologic sediment profiles. There have been successful restorations of this sort along San Francisco Bay.
Manual introduction of salt marsh plant species may be necessary if there is no abundant source. In the northern Gulf of Mexico, conversion of fresh marshes to salt marsh has occurred well (often irreversibly) within a few years time. Conversion of cypress forests have converted to salt marsh within a decade with salt water introduction.
Water level manipulation has had conflicting results, with largest problems being the ability to adjust structures regularly to suit needs.
Sources in the public domain you may find helpful are:
http://lacoast.gov/ (as a source of for 37 Hydrologic Restoration projects)
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I'm a little confused about the inter-changeability of these terms in a marine sedimentation context. My understanding is as follows:
Glaciogenic sedimentation = sedimentation derived from glaciers (or ice-sheets)
Glacimarine sedimentation = sedimentation derived from glaciers (or ice-sheets) that calve directly into the marine environment.
If this is correct then glacimarine sedimentation is a subset of glaciogenic and either of the terms could be used when referring to an ice-sheet that extends into the sea.
Can anybody provide some clarification?
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I don't think you need any clarification, your succinct definition is right on the mark. In "Glacimarine Environments: Processes and Sediments," Dowdeswell and Scourse (1990) defined glacimarine as "all those areas where sediment is deposited in the sea after release from glacier ice (including tidewater ice fronts, floating glacier tongues, ice shelves and icebergs) or sea ice." Since marine basins are more highly represented in the geologic record, even though this can be indeed be considered a subset of glacigenic, it is an important area of study. The book "Climate Modes of the Phanerozoic" (Frakes, Francis, and Syktus, 1992) is full of references to using marine sediments with increases or decreases of ice-carried dropstones as a proxy for ice-volume change.
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I am planning series of experiments in which I’d like to measure concentrations of N-15 labelled ammonia and urea in solution. I basically know that it is possible to measure concentrations of these compounds, and that it is possible to measure N-15 signature for all dissolved N-compounds. However, is it possible to measure this for each compound separately? If so, how do you do this? Could you recommend some literature?
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There is another diffusion method for 15N-NH3 where you buffer your sample with MgO to a pH of 9.5 to 10, which also converts the NH4+ to gaseous NH3 which can then be captured on a filter as ammonium sulfate. The filter is acidified beforehand with Sodium Bisulfate, and is made into a "filter packet" that floats on the aqueous phase. Diffusions last from 3 to 10 days depending on the concentration of ammonia, and the filters can then be dried and analyzed via EA-IRMS. Make sure to preserve your samples at pH2 with H2SO4 to prevent any loss of NH3 beforehand (although freezing also works). volatile organic nitrogen compounds can be a problem though
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My research facilities only limited to an IRMS and GC-MS without coupling. I am having trouble to use these instruments in finding the isotope ratios and concentration of halocarbons in the solid and aqueous samples.
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You may try MS for measuring 13C ratio.But it is rather tough for sample preparation than GC-IRMS. The best choice up to date is GC-IRMS.
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Method of digestion for total nitrogen and total phosphorus in sediment, how to extract different forms of nitrogen and phosphorus in sediment.
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For total N and P in highly organic soils (peat), I have used hot acid and hydrogen peroxide digestions. The approach might work pretty well in your sediments? The hydrogen peroxide breaks down the organic matter, releasing organic N as ammonium and organinc P as phosphate. Then I used colorimetric procedures to analyze ammonium and phosphate. I've also used ICP to analyze for phosphate in these digests. Unfortunately, I do not have my references handy to give you a citation, but perhaps I have given some information that will help you track down a reference. I have also used CHNS elemental analyzers to assess total N in this type of sample.
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I am trying to sample picoplankton (in the size range of 0.2 - 2 um) from the water column (tropical coral reef water) for stable isotope analysis and am wondering if anyone has suggestions on methods how to do this. For larger picoplankton it is possible to filter the water sample onto a GF/F filter (0.7 um), but this would miss a lot of bacteria in the water that are between 0.2 and 0.7 um in size. I have heard of people using aluminum oxide filters that are 0.2 um but these are really expensive. I'm wondering if anyone knows of an alternative type of filter to use or an alternative method.
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Best way to get the biomass you need is hollow fiber tangential flow filtration. Collect 200L of seawater in a poly tank through cartridge style pre filters to size fractionate the plankton (63, 10, 2 um for example) then run the <2 filtrate through hollow fiber cartridges to concentrate it down to a point where you can use a floor centrifuge to pellet it. Also see COFFIN, R.D., D. J. VELINSKY, R. DEVEREUX, W. A. PRICE, AND L. A. CIFUENTES. 1990. Stable carbon isotope analysis of nucleic ac- ids to trace sources of dissolved substrates used by estuarine bacteria. Appl. Environ. Microbial. 56: 2012-2020.
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I would like to correlate these parameters with sediment trap records near the mouth of the Gulf of Calfornia between 2002 and 2009.
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Thank you Peter for guidance.
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I intend to measure internal loading in a eutrophic subtropical lake.
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I see it in a paper, but i can not find it now.
I will send it to you when i find the paper.
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Hypoxia has become a worldwide phenomenon in the global coastal ocean, which affect the macrofaunal community for this connection lot of studies (Diaz and Rosenberg, 1995, 2008; Levin et al., 2000, 2003, 2009; Middelburg and Levin, 2009; Gooday et al., 2010; Ingole et al., 2010; Rabalais et al., 2010; Zhang et al., 2010). Is short term hypoxia showing any morphological, physiological and behavioural responses on them?
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Hi there, I guess it would depend on how you define short term, or what you mean with short term. How is short term hypoxia in your system? hours, days? Hard to say something about morphology, but physiologically it will depend on whether your species are adapted to hypoxic conditions, like under oxigen decreasing conditions they can activate anaerobic metabolisms pathways, so you can check on that enzimatic activity. No adapted species would simply avoid hypoxic conditions moving away from the site. Again, I guess it depends how short is the exposure to hypoxic conditions.
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Certainly so. The sheep of NZ and Australia have been blamed for supplying a considerable share of greenhouse gases by farting.
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I am coming across some synthetic fibres in the marine plankton samples collected from the water column approximately 10 deep and preserved in formalin. Some of these fibres (such as polyester and acrylic) are known to have been introduced into the marine environment as result of anthropogenic activities. But these fibres are also used in clothing and other fabrics and could also easily introduced into samples from the lab environment.
I was wondering if there is a way to distinguish the ones coming from the marine environment through some sort of chemical signature or trace elements unique to the marine environment?
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To begin with, i'll advice that it is the responsibility of a good scientist to handle confounding factors to the barest minimum, even if it means excluding the use of some fabrics from the lab.
Second, fibres from the marine environment will certainly have trace-metal associations or adsorbed metals that depict the environment it came from unlike fibres that originated from a terrestrial environment.
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Methylmercury concentrations of up to a few hundred femtomoles have been measured in the oxic water column of various ocean basins [1-15]. Maximum concentrations are found in the oxygen minimum zones. Oxygen is consumed here by bacteria during the remineralization of sinking organic matter. Yet only anaerobic bacteria and archaea have been shown to be capable of methylating mercury [16].
Refs:
1. Mason, R.P. and W.F. Fitzgerald, Alkylmercury species in the Equatorial Pacific. Nature, 1990. 347(6292): p. 457-459.
2. Mason, R.P. and W.F. Fitzgerald, Mercury speciation in open ocean waters. Water Air & Soil Pollution, 1991. 56(1): p. 779-789.
3. Mason, R.P. and W.F. Fitzgerald, The distribution and biogeochemical cycling of mercury in the Equatorial Pacific-Ocean. Deep-Sea Research Part I-Oceanographic Research Papers, 1993. 40(9): p. 1897-1924.
4. Cossa, D., J.M. Martin, K. Takayanagi, and J. Sanjuan, The distribution and cycling of mercury species in the western Mediterranean. Deep-Sea Research Part Ii-Topical Studies in Oceanography, 1997. 44(3-4): p. 721-740.
5. Mason, R.P., K.R. Rolfhus, and W.F. Fitzgerald, Mercury in the North Atlantic. Marine Chemistry, 1998. 61(1–2): p. 37-53.
6. Mason, R.P. and K.A. Sullivan, The distribution and speciation of mercury in the South and equatorial Atlantic. Deep Sea Research Part II: Topical Studies in Oceanography, 1999. 46(5): p. 937-956.
7. Monperrus, M., E. Tessier, D. Amouroux, A. Leynaert, P. Huonnic, and O.F.X. Donard, Mercury methylation, demethylation and reduction rates in coastal and marine surface waters of the Mediterranean Sea. Marine Chemistry, 2007. 107(1): p. 49-63.
8. Cossa, D., B. Averty, and N. Pirrone, The origin of methylmercury in open Mediterranean waters. Limnology and Oceanography, 2009. 54(3): p. 837-844.
9. Sunderland, E.M., D.P. Krabbenhoft, J.W. Moreau, S.A. Strode, and W.M. Landing, Mercury sources, distribution, and bioavailability in the North Pacific Ocean: Insights from data and models. Global Biogeochemical Cycles, 2009. 23: p. 14.
10. Heimbürger, L.E., D. Cossa, J.-C. Marty, C. Migon, B. Averty, A. Dufour, and J. Ras, Methyl mercury distributions in relation to the presence of nano- and picophytoplankton in an oceanic water column (Ligurian Sea, North-western Mediterranean). Geochimica Et Cosmochimica Acta, 2010. 74(19): p. 5549-5559.
11. Cossa, D., L.E. Heimbürger, D. Lannuzel, S.R. Rintoul, E.C.V. Butler, A.R. Bowie, B. Averty, R.J. Watson, and T. Remenyi, Mercury in the Southern Ocean. Geochimica Et Cosmochimica Acta, 2011. 75(14): p. 4037-4052.
12. Lehnherr, I., V.L. St. Louis, H. Hintelmann, and J.L. Kirk, Methylation of inorganic mercury in polar marine waters. Nature Geosci, 2011. 4(5): p. 298-302.
13. Cossa, D., M. Harmelin-Vivien, C. Mellon-Duval, V. Loizeau, B. Averty, S. Crochet, L. Chou, and J.F. Cadiou, Influences of Bioavailability, Trophic Position, and Growth on Methylmercury in Hakes (Merluccius merluccius) from Northwestern Mediterranean and Northeastern Atlantic. Environmental Science & Technology, 2012. 46(9): p. 4885-4893.
14. Hammerschmidt, C.R. and K.L. Bowman, Vertical methylmercury distribution in the subtropical North Pacific Ocean. Marine Chemistry, 2012. 132–133(0): p. 77-82.
15. Wang, F., R.W. Macdonald, D.A. Armstrong, and G.A. Stern, Total and Methylated Mercury in the Beaufort Sea: The Role of Local and Recent Organic Remineralization. Environmental Science & Technology, 2012.
16. Parks, J.M., A. Johs, M. Podar, R. Bridou, R.A. Hurt, S.D. Smith, S.J. Tomanicek, Y. Qian, S.D. Brown, C.C. Brandt, A.V. Palumbo, J.C. Smith, J.D. Wall, D.A. Elias, and L. Liang, The Genetic Basis for Bacterial Mercury Methylation. Science, 2013.
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That's a great question. So far we have the following hypotheses:
1. photochemically-driven abiotic methylation
2. methylation in anoxic microenvironments
3. methylation by phyto/bacterio plankton
4. methylation by anaerobic bacteria tolerant of oxic conditions
I think that we can probably rule out #1, as a number of papers (e.g., Black et al.) have shown that photochemical processes result in net demethylation and not net methylation. I think that #2 is a sound hypothesis...for example, particles can provide all the right conditions to promote Hg(II) methylation, including anoxic microhabitats, a source of Hg(II) and a source of organic C to fuel microbial metabolism. Someone above ruled out #3, but I would be curious to find out why. It has been previously suggested that Hg(II) methylation could be linked to the sulfur cycle and plankton-produced DMS, as there are a number of methyl transfer reactions linking DMSP, DMS and MeSH which could potentially result in Hg(II) becoming methylated (see for example, Larose et al 2010). I don't know enough about #4 to comment, but I would think that methylation of Hg(II) in marine waters is probably the result of a combination of hypotheses #2 and #3, although I would love to hear more people's opinions on this topic.