Questions related to Marine Algae
I came across this seaweed at mid to low tide level on the rocky shores of Sindhudurg (Central West Coast of India. Is this a Valoniopsis pachynema?
Hi, when I DNA extract from sargassum (brown algae), I did not see any band on agarose gel or band is very weak. please help me.
Bioinsectisides are popular now for crop protection. The proponents would like to ask some recommendation for capturing VOC compounds from marine algae the is easy. What formulation of non-toxic aerosol type bioinsectiside can be compatible for VOC.
Active Ingridient - VOC from Marine Algae
Inert Ingridient - ???
I came across this seaweed at mid to low tide zone on rocky shores of Sindhudurg (Central West Coast of India). More prominently seen during monsoon season. I feel this is a Chaetomorpha antennina.
Hello, can somebody help me to identify this specie of Chlorphyte? I find it in a reef in front Veracruz and I think it´s a Caulerpa, but I am not sure.
Thank you for your help.
I was looking for Caulerpa lentillifera earlier today in Kenyan South Coas in Kibuyuni, Kwale county. I found a different kind of sea grape. I am certain that it is of the genus Botryocladia, but I am uncertain of the species. Does anyone have an idea about this macroalgae?
I hope the photos are clear enough.
Also, has anyone come across Caulerpa lentillifera in the Kenyan coast? Please let me know.
What is the criteria should I follow to study the antioxidant effect of an extract of marine algae?
I mean determination of the concentration that recommended to be administrated in water.
Is there a standard method should I follow?
Should I perform a toxicity test first?
Could I use the concentration to the its effect on amino acids and fatty acids metabolism of adult Zebrafish?
This can be detailed or anecdotal, quantitative or qualitative. Over the course of years or decades. Anything that reports changes in the size of the canopy forming species.
I am trying to obtain polysaccharides from several unicellular, eukaryotic marine algal cultures, without any intact cells (or salt). Purity is not my hugest priority - I don't mind getting a few proteins or other molecules. I also don't mind if the polysaccharide comes from within the cell. I've tried prefiltering with 0.45 and 0.22 micons, then preciptating with ethanol, methanol, calcium chloride, and calcium hydroxide, and none of these seem to work. I'm considering just busting the cells open at this point and getting any polyacrylamide I can get. I'm using total carbohydrate assay to measure if I got anything, and eventually Alcian Blue. I've been optimizing with Chaetoceros tenuissimus. Any help would be greatly appreciated!!
I was wondering about the role of free fatty acids in dinoflagellates, more specifically the symbiodiniaceae? What are the metabolic pathways and where are the different types of lipids usually manufactured?
Dear RG Colleagues,
I am doing a survey on the plant material (species, genus ...) which is the subject of several field of research in each department/city/country. Your contribution will be a great help. Best regards Abdenour
It looks like the bigest known centric diatom is Ethmodiscus rex, which frustule can be bigger than 1 mm.
Do you know the other biggest centric diatom(s)?
Which centric diatoms are the smallest?
It is experimentally proved that turf algae in combination with sediment prevents the settlement of coral larvae. My field observations are contradictory to it. I observed lot of new recruits on hard substrate which has been covered with turf algae and sediment. Is there any other factor which could aid the settlement of coral larvae on a turf algal substrate?
We are trying to produce in the laboratory gametophytes and sporophytes of Laminaria digitata and Saccharina latissima together with Palmaria palmata. Still we are struggling to get rid of the diatoms present in our fertile material. Even pre-treatments with iodine or bleach, as well as manual scrubbing and several washes with filtered and autoclaved seawater do not help to solve the problem. Thanks to everyone that can give a hand for this!
I am working on isolating fatty acids from marine algal extracts. GC-MS results show the presence of fatty acids I am looking for. But the fatty acids don't get separated on don't even move on column. I tried all levels of solvents from Pet Ether to Methanol-Chloroform system. But no result. Where am i going wrong?Or is there any other approach to isolate them?
Can anyone help me to identify two species of Codium found in Sayulita, Nayarit (Mexico)? I made a cross section to watch the utricules in an attempt to identify them using a bibliography but I still found it difficult.
The dark green one is A)
The light green one is B)
I would like to know if there are online available and free world-wide datasets of sea surface temperature, net primary productivity, salinity, pH and dissolved oxygen.
I will be going into my 3rd year of study in September and have decided to do an experimental project in the areas of Microplastics in water bodies. Does anyone have any suggestions for topics? I am struggling to think of a specific title within that area. This sort of project would have a 10 week time limit so it would have to be suited to fit that timescale.
I have thought of microplastics in phytoplankton, sediment samples, freshwater samples and possible drinking water samples as well.
Hi, I'm Minjae.
My sample is a Dunaliella, the green microalgae growing well on salinity culture media including 0.6M NaCl. In these days, I'm searching on the ROS detection assay which is possible to apply on my cell.
I used DCFDA cellular ROS detection assay kit (ab113851, abcam).
I modified several experimental conditions to fit on my sample.
1) Because this cell is cultured on salinity culture media, it could be affected by osmotic pressure. So, I couldn't use DW to dilute 10x buffer. (Manufacturer's protocol recommends the dilution of 10x reaction buffer into DW.) Instead of DW, I used the salinity culture media inculding 0.6M NaCl. "Is it possible to use salinity culture media, instead of DW?"
2) There are many reports using this ROS detection assay kit for animal cells which do not have pigments (autofluorescence). However, Dunaliella has green autofluorescence induced from a chloroplast. To solve this problem, I measured the value of resuspended cell into 1x supplemented buffer as a negative control, and calibrated the value based on the values of negative control.
I attached my result. The Dunaliella cells (A, B) were exposed to high light condition to make them ROS generation. From my result, it showed that A(red) and B(blue) have similar ROS contents at the 20uM DCFDA treated conditions.
To confirm that the kit was applied well on my cell, TBHP was treated to the sample. The value was increased than DCFDA samples, and it seems that the kit is working well in my case. All values were calibrated to relative value than none (without 20uM DCFDA).
The data showed significant differences between negative controls, but I'm still not sure with my data. Because its signal intensity is too low compare to other results from animal cell lines. "I know that it is a stupid question... how much level of intensity is necessary to show the data meaningful?"
Any suggestions on making an aquarium chiller or are there any cheap and reliable ones out there that I could buy?
Has anyone made their own for culturing experiments?
I used dried and ground Sargasssum spp. to enrich surface sediments. Took sediment samples (that may have bits of Sargassum spp.) and determined chlorophyll-a spectrophotometrically. I am wondering if the chlorophyll-a could come from the dried Sargassum ssp., and not benthic diatoms.
I have attached herewith, the paper by Pirbazari et al., (2014) where the Foumanat tea waste was alkaline treated. The SEM images thus presented have clear differences between the treated and the untreated biomass. But one of the features that have not been discussed upon is the flaky material present in excess in the untreated biomass but absent/ present very little in the treated biomass.
I have made the similar observation in my study. My doubt is as to which component of the plant material is this? NaOH has removal effect over lignin and part of hemicellulose. Is it one among the two or something (some other plant component) else that I have missed considering?
I would be grateful to any suggestions that would make the discussion complete and clear.
PS. I have added a sticky note on the flaky material in the SEM image of the attached paper.
Need help in phytoplankton identification: this round-shaped thing was seen under 40* objective: sample collected during a red tide at early summer of the east china sea, at a density of about 4* 105 / L, dominant species co-identified was Gonyaulax spinifera (color of tide was rather light, with large amount of foam)
Attached please find some figures representing a species of filamentous and mat forming species of Cyanobacteria collected from the Upper intertaidal rocks of the eastern Medit Sea. The color of this species brown to yellow to somewhat gold. First, I believed that it is a species of the Oscillatoriales but I am not quite sure anymore since the apical cells looks different. Can anyone help me identifying the genus and may also speculate the species. It's hard to identify Cyano, I know... Ohh, here are some more details: Trichoms are cylindrical, about 12-13 µm in diameter and (1.5-) 2-4 (-5) µm thick.Tubes are up to 18 µm thick. Scales of figs. are 50 µm or 200 µm (1 fig). Thank you very much.
I have old 150-180 ml water samples that have been fixed in formalin. The goal is to document changes in anabaena (dolichospermum) strains as well as in diatoms starting from 1977 to present day. The trick is that the anabaena colonies need to remain intact. This is promlematic because of the size of the samples and the fact that the anabaena floats but the diatoms do not. I am also afraid that filtering will ruin the colonies. Note: These are just plain water samples, no plankton net was ever used.
Does anyone have any ideas on how to achieve this?
Hello everyone, I try to sieve protozoa, fungi and bacteria from soil solution with different sizes of meshes, then wash them off from the mesh, so far i dint find useful literature about this, anyone has some ideas ? thanks a lot!
During the last summers, extensive blooms of mucilaginous algae have been observed along the French Mediterranean and NW Italian (Liguarian Sea) coasts. Does anyone have information about a similar phoenomenon along spanish Mediterranean coasts?
I know it grows there but that is according to a fish pen breeding website that lists it as a food for milk fish. http://www.fao.org/docrep/003/W6928E/w6928e07.htm. but I cannot find any recent surveys/biological inventories regarding the algae of Laguna Lake.
We are looking forward to perform a genomic DNA extraction for a whole genome sequencing on a monoculture of algae such as Dolichospermum and Microcystis and on the diatom Nitzschia. What is the recommended gDNA extraction kit for species such as these? Will the gDNA extraction kit differ when you're looking at a different genus or species?
We are preparing an application for the study of these algae in the next four years (1918-2021). The main questions are: a) optical properties of habitat; b) relations with Cyanobacteria; c) special food web on the basis of large green algae. Colleagues interested in cooperation or presenting questions are welcome!
Culturing of pure or dominant Moina in an earthern pond has been very difficult as copepods tend to grow and become the dominant species within a short period.
Are there any method to eliminate copepods but remain Moina? or separate copepod and Moina?
They are from a TEMPORARY STREAM from south Spain. Fixed by ETHANOL
Colonies size 5 a 10mm, mucilaginous colonies floating through Rannunculus vegetation
Cell size around 9-10micras. distributed in 4 celled groups surrounded by a mucilage,with fragments of remains from mother cell wall.
thanks in advance
Can anyone share me the publication "Planktonic dinoflagellates" (by Hallegraeff et al., 2010)?
Hallegraeff GM, Bolch CJS, Huisman JM, de Salas MF (2010) Planktonic
dinoflagellates. In: Hallegraeff et al, editors, Algae of Australia phytoplankton of
temperate coastal waters. CSIRO Publishing / ABRS. Melbourne. pp. 145–212.
I'm working on the effect of silica levels on marine polar (Chaetoceros neglectus in Southern Ocean) and tropical diatoms (Actinocyclus octonarius in Malaysia).
I'd like to know the current composition and the threats of silica in polar and tropical waters.
Those dinoflagellates founs in the Black Sea. I think they are Prorocentrum, but could not saythe exact specific name. May be they belong to P. compressum.
Does anybody know the specific name of that dinoflagellates (Prorocentrales)?
I am thinking to make supercritical Carbon dioxide extraction technique more economic and sustainable by add value to products that do not have value.
One of the products will be Tetraselmis suecica (marine algea), I will extract lipid from the biomass, and I need to isolate polysaccharides from the rest product (mucilage), as far as I know it is a mix of protein and polysaccharides?
The impact of ecological factors on the content of bioactive compounds sulfated polysaccharide in brown seaweed