Science topic

Mammals - Science topic

Warm-blooded vertebrate animals belonging to the class Mammalia, including all that possess hair and suckle their young.
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i need to find the common regions between them to make a tree that describes the relation between them or may i use specific gp of genes in the comparison instead of the whole genome?
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i have tried MAUVE but the resulted tree isn't like the real tree of life, but i will try ARTEMIS @Robert Adolf Brinzer
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I am looking for data from mammals ideally, but I will take anything to be honest. I am getting to grips with bioinformatics and need a practice data set with which I can go through the steps of filtering and trimming and mapping to a reference genome etc..
If anyone also has any advice on tools used subsequently for analysis such as MethylKit that would be awesome.
Thank you
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Dear Katharyn following link might be useful for you, just try it.... as it has all the downloadable contents including the fastq files you may need in methylation work...
Regards....
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Hi everyone 😉
Does anyone have any tips for a good book that has lots of information about wildlife. Preferably mammals that live in Europe.
Thanks in advance !😀
kind regards
Anouk
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Will do!
You may contact me anytime! I love to learn!
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The Pfizer mRNA vaccines contain
(4-hydroxybutyl) azanediyl) bis(hexane-6,1-diyl) bis(2-hexyldecanoate) ALC-0315
also known as 6-[N-6-(2-hexyldecanoyloxy)hexyl-N-(4-hydroxybutyl)amino]hexyl 2-hexyldecanoate, sold as a yellow oil.
It has 2 chiral centres so potentially the different optical isomers will have different toxic properties in Humans as found for Thalidomide.
Has any effort been made to separate isomers?
ALC-0315 has not had detailed toxicology studies, however in rats the half-life for transfer from blood to other organs was 139 hours, indicating it is very strongly bound inside the bodies of mammals. Would there be any difference in binding and metabolism of the isomers?
Which enzymes or other essential biological molecules might be expected to interact with ALC-0315?
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Hi,
What we know from available documents ALC-0315 is 1:1 racemate, and a statement from the document below explains that racemate was used:
"This excipient contains two chiral centres, but the excipient is a 1:1 racemic mixture of the diastereomers. The excipient is not optically active."
In regards to impurities of ALC-0315 looks like additional data was collected a year ago. But I'm not sure the data are publicly available:
"5) The sponsor to provide a discussion regarding process development for ALC-0315 with emphasis on the identification and purge of impurities. Due date: July 2021.
6) The sponsor will notify Medsafe of any changes to the ALC-0315 manufacturing process and/or suppliers/manufacturers/testing sites using Changed Medicine Notifications.
7) Specified impurities should be further evaluated and appropriate specification limits for individual impurities should be included when more data are available. Acceptance criteria for specified and un-specified impurities should be added to the specification for ALC-0315 and should also be evaluated during stability studies. Due date: July 2021; Interim report: April 2021 (...)
9) The sponsor to update the ALC-0315 assay and impurities limits when additional supporting data is available. Due date: July 2021."
Ref:
  1. https://www.health.govt.nz/system/files/documents/information-release/h202106950_response.pdf
  2. https://www.ema.europa.eu/en/documents/assessment-report/comirnaty-epar-public-assessment-report_en.pdf
One comment related to the long half-life. It could be possible in cases when the specific molecule is present in the bile that enterohepatic recirculation of the substance is possible. It can explain extremely long half-lives sometimes. Of course in some optically active substances, the process could be enantioselective (chiral inversion).
As we know ALC-0315 is a mixture of the diastereomers: "Diastereomers are any molecules which have two or more chiral centers. A diastereomer with two chiral carbon has four isomers. Unlike enantiomers, the physical and chemical properties of diastereomers can differ and consequently, their chemical characterization is easy and their biological activities are often different."
Hope could be helpful for future discussions,
Best regards,
Tomasz
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I would like to understand how different load on the muscle affects the development of bone tissue, the presence of tubercles on the bones, thickening, etc.
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Attached is a review article we wrote several years back about the linkage of muscle loading to bone properties and how that changes with aging.
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Hi, do you guys know some experts in isolating novel/new/unculturable bacteria from mammals?
Thank you in advanced
Best regards
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search google scholar
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I have studied seasonal multiscale space use patterns of mammals through camera traps implementing a GLMM framework, and took site as a random factor. I used all GIS-based covariates (except for one i.e. human photo-capture rate as a surrogate for disturbance) in this analysis, and found significant relationships between mammal occurrence and some of the covariates. In this context, I was wondering if I can prepare a habitat suitability map out of it (the best-selected models), it would be really great, but unfortunately, I couldn't find enough literature that can actually guide me.
All comments are welcome and thank you in advance.
Sankarshan
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JSDMs are hierarchical models; GLMM is just another name for these kinds of models.
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Hi. I have a question of the MHCII expression in mammal. What I know so far is that the expression of MHCII is codominant, alleles from both mom and dad will be expressed. However, I would like to if the genotype of MHCII affect the phenotype. For example, would the MHCII wild type animals express more MHCII protein than the MHCII heterozygous animals? Also, would the MHCII WT animals process more antigen and have a stronger immune response than the MHCII heterozygous animals? Assuming only one specific type of antigen is processed in this case.
It will be very helpful if you can post some related literature. Thanks for your time!
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interested
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We are trying to persuade farmers and hunters not to kill wolves, lynxes, bears, otters, and beavers. But we need a financially based argument that these mammals are beneficial for the ecosystem and save money if we keep them alive. Is there any literature that estimates how much money will one wolf/lynx/bear/otter(/beaver save per year?
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I agree with Kewan Mertens .
Accepting to monetize living organisms is equivalent to supporting the capitalist model, which is at the origin of the destruction of many ecosystems and species.
In my opinion, we should not play this game and work more on scientific arguments (limitations of rodent populations, limitations of the spread of certain diseases).
Some interesting books or articles for this debate:
Jiguet, F. (2020). The Fox and the Crow. A need to update pest control strategies. Biological conservation, 248, 108693.
Hofmeester, T. R., Jansen, P. A., Wijnen, H. J., Coipan, E. C., Fonville, M., Prins, H. H., & van Wieren, S. E. (2017). Cascading effects of predator activity on tick-borne disease risk. Proceedings of the Royal Society B: Biological Sciences, 284(1859), 20170453.
Maris, V., 2014. Nature for sale. The limits of ecosystem services. Quae, Versailles. (in French)
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When pathogens from diseased mammals are isolated with Vero cells or other cell lines, the probability of success is very low. What are the suggestions for cell culture and pathogen isolation?
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Follow the steps of isolation and identification of the standard protocols for each pathogen, as well as taking into account other physical and chemical conditions that may affect the isolation rate.
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I need to compare Tgf-beta in fish and in mammals and I need a western blotting kit that can target the protein sequence with equal accuracy?
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Katie A S Burnette I meant to say protien😅.. I guess I was thinking of the alternative for the western blotting when I wrote the question!
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we are studying the effect of the presence of the golden jackal in the northeast of Italy on a red fox, wild cat and ungulates and other species; can you share any ideas, data or observations on the role of the golden jackal on the dynamics of mammals, of medium and large size, and birds?
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They occur sympatrically with wild and jungle cats in Turkey even when they are at high densities. Also not much influence on presence of ungulates and birds as they are generalist carnivores and they consume a wide variety of food sources from fruits and anthopogenic waste to carcasses and opportunistically (seldomly) on newborn ungulates.
My long term camera trapping data (unpublished) and also data I collected at multiple study sites shows that where jackals are at very high densities foxes are either absent or occur at very low densities. If you'd consider a comparative study (Italy vs Turkey) I can provide data, observations and experience on this. Good luck!
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Climate change is a prolonged process and does not affect an ecosystem suddenly. Camera traps do not provide any information regarding how precipitation and temperature is fluctuating daily, seasonal, or yearly. In this case, which alternate method could be more effective to monitor the impact of climate change on hibernation in mammals?
But If camera traps could also be used two questions arise here:
what should be the duration of the study? I don't think 3-4 years of monitoring could be enough to get reliable results. Secondly, only camera traps are enough to monitor the animals?
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Dear Kurt
I think no. Relying on temperature recorded by camera traps can not provide provide the climate change data. Camera traps could only record temperature when capture an animal while passing in front of it. Climate change is something els. Its an overall change and needs long term data
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Paragonimiasis is a parasitic disease that can be transmitted through the consumption of infected freshwater crabs by mammals including humans. Infective larval forms of the parasite has been found in crabs but not so much adults were found in humans. Therefore, I think that the other mammals in the vicinity of the freshwater crab distribution must be responsible for the continuation of the life cycle of Paragonimus westermani. However, I need to find out if the wild mammals near freshwater bodies are infected with the parasite by examining their stools for parasite eggs. How can I do this?
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The most likely mammals to eat freshwater crabs would be otters, civets, possibly the stink badger, rodents, moon rats and shrews. Otter and civet faeces are easily recognizable in the field - look on-line or, better, get someone to show you. For rats, moonrats, and shrews, I would live-trap them them and collect faeces from the trap.
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My study revolves around the cuvier's gazelle ( Gazella cuvieri ) in its natural habitat.
How can I get the most of my days in the field, and come back with as much information as I can.
A precise protocol would be appreciated, the study focus is population dynamics and overall population behavior.
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It depends on the objective of the study. The focal animal sampling method is commonly used to observe different behaviour traits of ungulates, e.g., foraging, social interactions, etc.
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Mammals ravage the nests of artificial nesting birds. There are probably modern methods that display data from burglary attempts, etc.
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You could also make nutritional analyes of the predators (feaces, stomach contents). And of course direct controls like Johannes Mayer mentioned or Thermo loggers Florian Straub mentioned. However , nest observation with Fototraps or even better with video observation is the most precise method but with the highest workload.
I have not the rights to upload the texts here, but see:
ZSCHILLE J, STIER N, ROTH M, MAYER R (2014): Feeding habits of invasive American mink (Neovison vison) in northern Germany—potential implications for fishery and waterfowl. Acta Theriologica 59 (1), 25-34. https://doi.org/10.1007/s13364-012-0126-5.
STIER N, ZSCHILLE J, ROTH M (2005): Untersuchung zu den gebietsfremden Raubsäugern Marderhund, Waschbär und Mink in Mecklenburg-Vorpommern mit Forschungsschwerpunkt Mink. Zwischenbericht. Institut für Forstbotanik und Forstzoologie, TU Dresden. 21 S.
VOIGT U, SIEBERT U (2016): Prädation Niederwild. Abschlussbericht für die Untersuchungsjahre 2011-2015 Niedersächsisches Ministerium für Ernährung, Landwirtschaft, Verbraucherschutz und Landesentwicklung. Institut für terrestrische und aquatische Wildtierforschung, TiHo Hannover, Hannover. S.
VOIGT U (2016): Prädation in der Kulturlandschaft. Abschlußbericht Niedersächsisches Ministerium für Ernährung, Landwirtschaft und Verbraucherschutz. Inst. f. Terrestrische und Aquatische Wildtierforschung, Hannover. S.
KÄSELAU S (2021): To what extent do stomach content analyses provide information on predation on ground-nesting birds? MSc. University of Veterinary Medicine Hannover. 31 S.
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" Given lenalidomide’s mechanism of action, it is intriguing that IMiDs and proteasome inhibitors are synergistic in the treatment of multiple myeloma.(24) Recent evidence suggests that this synergy may result from the pharmacokinetic properties and dosing schedules of these drugs. Although treatment with proteasome inhibitors can block lenalidomide induced degradation of IKZF1 and IKZF3 in vitro,(14) this effect depends on both the order of administration and the dose. When the drugs are administered at the same time, lenalidomide-induced degradation occurs before the onset of proteasomal blockade. (25) " excerpt from [1] below.
[1] BLOOD SPOTLIGHT| NOVEMBER 19, 2015
The novel mechanism of lenalidomide activity
Blood (2015) 126 (21): 2366–2369.
" In this article, we review the recently recognized mechanisms of action of lenalidomide and discuss the most recent clinical data regarding its use in patients with both 5q− MDS as well as non-5q− MDS. " excerpt from [2] below.
First published: 13 February 2017
[2] Lenalidomide use in myelodysplastic syndromes: Insights into the biologic mechanisms and clinical applications
Maximilian Stahl MD,Amer M. Zeidan MBBS, MHS
[3]Jacob Keevan & William D Figg (2014) , Cancer Biology & Therapy, 15:8, 968-969, DOI: 10.4161/cbt.29189
Updated Dec 26th 2021 as I know have some publications of relevance to Lenalidomide. Top of my list are [1] and [2] at the moment they are most recent publications I have found. [3] added Jan 24 2023 - still need to review but it pre-dates [1] and [2]
Some additional references for background.
Damato AR, Katumba RGN, Luo J, Atluri H, Talcott GR, Govindan A, Slat EA, Weilbaecher KN, Tao Y, Huang J, Butt OH, Ansstas G, Johanns TM, Chheda MG, Herzog ED, Rubin JB, Campian JL. A randomized feasibility study evaluating temozolomide circadian medicine in patients with glioma. Neurooncol Pract. 2022 Jan 31;9(3):193-200. doi: 10.1093/nop/npac003. PMID: 35601970; PMCID: PMC9113320.
Core Concept: Emerging science of chronotherapy offers big opportunities to optimize drug delivery https://lnkd.in/gpAz-28v
Optimizing circadian drug infusion schedules towards personalized cancer chronotherapy
Hill RJW, Innominato PF, Le´vi F, Ballesta A (2020) PLoS Comput Biol 16(1): e1007218. https://lnkd.in/gnCAAzk5 https://lnkd.in/g4sY-NDv
Temporal regulation of tumor growth in nocturnal mammals: In vivo studies and chemotherapeutical potential
The FASEB Journal. 2021;35:e21231.First published: 11 January 2021 https://lnkd.in/gm9mr6z
Paula M. Wagner,César G. Prucca,Fabiola N. Velazquez,Lucas G. Sosa Alderete,Beatriz L. Caputto,Mario E. Guido
Harnessing the predictive power of preclinical models for oncology drug development. 
Honkala, A., Malhotra, S.V., Kummar, S. et al. Nat Rev Drug Discov (2021). https://lnkd.in/gYnT7VtF
Updated Aug 22nd 2022: Chronotherapy Clinical Trial with a different Drug which is a good background resource.
Temozolomide Chronotherapy for High Grade Glioma
Updated Jan 12th 2023: Wang, Chen, et al. "The circadian immune system." Science Immunology 7.72 (2022): eabm2465. Another good background resource.
Article The circadian immune system
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I have treated 600 cancer patients with thalidomide and celecoxib, using thalidomide 200mg before sleep, because it has the activity to let the patients somnolent. Celecoxib was able to give an effective therapeutic effect at 400 mg twice a day. As a result, the usage would have been effective.
I expect thalidomide and celecoxib to be marketed worldwide as anti-cancer agents and as a useful drug for the treatment of COVID-19 infections.
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I would like to answer the question about the absence of top predators effect on mammal community by using camera trap data. So,
1. What kind of study design, method and analysis are suitable to solve this question?
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You will need to evaluate the effects of the other variables, such as the effect on climate, perpetuation (of the specie) and above all how "easy" for a top animal to gain all its needs of the surroundings. Has the animal been succesful in survival? Does he have a clear advantage?
Maybe the studies conducted would be amongst population genetics, statistics and maybe in terms of Darwin, even though it is not very precise.
I think they are people that can explain this better.
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Hello Friends,
I am curious to know what kind of test can we apply for this dataset. Columns are categorical (Study site) variables. the active number indicates the sighting of a particular mammal species.
Rows are different types of tree species.
Any help would be appreciated
#Chi-Square #Statistics #Descriptives #ANOVA
Thanks
Thayyib
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Guillaume Adeux: Only these six sites have a mammal species. These six SGs have diverse plants. Hence, we are looking for their behaviour such as feeding, nesting. Is there a statistical test other than Rank Correlation or Kandell Tau?
I am a finance researcher by practice. So, I admit I am not good at conservation and biodiversity statistics.
 My sister is currently working on a research paper based on her PG dissertation.
Thanks again
Thayyib
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It has long been recognised that bumble bees most often nest in abandoned mammal (usually rodent) nests, and I believe various researchers have tested the effects of mammal scent in attracting queens to field hives, but I'm not aware of any positive results. However I have not been keeping up with literature for a while and may have missed it.
Do you know of results confirming the effect of mammalian odours?
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Dear Nelson,
this is a very interesting technical question which I personally never thought of. To my knowledge, the reason why bumble bees very often nest in abandoned mammal (mice and voles) nests is that they cannot dig themselves. According to many links available on the general internet it is generally accepted that some bumble bees can faintly smell the mice and voles that once built the nest and follow this scent until they find the entrance. Unfortunately I could not yet access a scientific article in which this behavior has been proven.
Good luck with your work!
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Which would be inverse to the way metabolism scales according to Kleiber’s Law, that is, inverse to the way metabolism scales as mass to a 3/4 exponent?
Which would be inverse to the way metabolism scales according to Kleiber’s Law, that is, inverse to the way metabolism scales as mass to a 3/4 exponent?
So that the rate of metabolism scaled, times longevity scaling is invariant?
Has this hypothesis been proposed? Are there are articles on the scaling of mammalian longevity?
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I could not tell about the longevity scale for mammals as a function of mass.
However, there is a known longevity scale for the number of heartbeats per lifetime for animals as a function of weight in kg.
See, for example, Figure 2 in Geoffrey West, Scale: The Universal Laws of Life, Growth, and Death in Organisms, Cities, and Companies, Penguin Books; Reprint edition (May 15, 2018), p.3.
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Say a researcher was interested in determining the number of adults vs. juveniles of species X trapped during a small mammal survey. Does there exist a relatively reliable way of doing this based on standard field measurements?
Let’s say a total of 200 individuals of species X were sampled, and the following data recorded: sex, total length, tail length, hind foot length, ear length, and weight. For the sake of this question imagine no additional data is available (e.g. additional observations recorded in the field, access to collected specimen material, etc.).
  1. Is there a way to ascertain a point or “threshold” from a range of data based on the distribution of values to distinguish between juvenile and adult individuals with a meaningful degree of accuracy? For example, male species X with weight > 142 g = adults; < 142 g = juveniles.
  2. If yes, which of these measurements would be most indicative? Or perhaps a combination/ratio of more than one (e.g. ratio of hind foot length to ear height > 1 = adult, etc.)?
Thanks, and looking forward to the feedback.
Evan
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Search :
Crouched Locomotion in Small Mammals: The Effects of Habitat and Aging
by Angela M Horner (2010)
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The goal of the project is to collect around 200-250 samples (primarily muscle tissue) from a bushmeat market and transport to laboratory for species barcoding. DNA extraction will then be performed, followed by PCR using universal mammalian primers (and if no product is yielded, universal vertebrate primers), confirmation of product using agarose gel electrophoresis, DNA purification, Sanger Sequencing, then alignment to the lowest possible taxonomic unit. The results will be compared to reported species by the seller (to assess misidentification) as well as used to identify protected species.
Current plan is to first use the following universal mammalian primers (majority of samples expected to be mammals):
MTCB-F (Size~1420) 5'-CCHCCATAAATAGGNGAAGG-3', targets cyt b (Naidu et al 2012)
MTCH-R (Size~1420) 5'-WAGAAYTTCAGCTTTGG-3', targets cyt b (Naidu et al 2012)
Then if those don't yield products use the following universal vertebrate primers:
L1085 (Size 215) 5'-CCCAAACTGGGATTAGATACCC-3', targets 12S rRNA (Kitano et al 2007)
H1259 (Size 215) 5'-GTTTGCTGAAGATGGCGGTA-3' targets 12S rRNA (Kitano et al 2007)
Question: This is my first time performing species barcoding of any kind (and also only have minimal bench experience, background is clinical); are these appropriate primers? Any critique/advice on methodology?
Thank you so much!
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U can also try by using cytochrome c oxidase I (COI or COX1) gene.
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There are two extant species of Hydrochoerus: Hydrochoerus isthmius, the lesser or Panamanian capybara, and the genotype species H. hydrochaeris. The latter is the more common species of capybara, found throughout most of South America, whereas the other is restricted to the northwestern side of the Andes ranging into Panama. However, H. hydrochaeris doesn't seem to have a useful common name to distinguish it from H. isthmius. It's referred to as the "capybara", but both species are capybaras, and it's never referred to as the "common capybara", "greater capybara", or "southern capybara". H. hydrochaeris is also much larger than H. isthmius (nearly twice the size of the latter species).
I am making a figure I intend to use to show to an educated layman audience, and am using capybara bones as an extant scale. I am trying to use the common name to not confuse my audience, but at the same time I want to make it clear I am referring to H. hydrochaeris and not H. isthmius so there is no confusion for people who are more familiar with scientific names. Given this, what would be the correct common name to refer to H. hydrochaeris such that I do not confuse my audience?
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The capybara, capybara, chigüiro or chigüire, which is a species of rodent of the Caviidae family typical of South America, being the largest rodent in the world
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Xylazine is an analogue of clonidine and an agonist at the α₂ class of adrenergic receptor. It is used for sedation, anesthesia, muscle relaxation, and analgesia in animals such as horses, cattle and other non-human mammals. Veterinarians also use xylazine as an emetic, especially in cats
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This drug can significantly lower blood pressure and negatively impact central nervous system function. It can decrease a person’s respiratory rate and heart rate to dangerously low levels. According to data from the Philadelphia Medical Examiner’s Office, xylazine has been part of 31% of all unintentional overdose deaths where heroin or fentanyl was identified. Re­cent reports indicate that it is increasingly being mixed with illegal drugs, such as heroin, to increase the effects of the high. Xylazine is not a controlled substance and is often easy to obtain from a veterinarian. So, it has been highly recommended that veterinarians who use xylazine be vigilant of this new information and remain conscientious about its abuse potential to humans.
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I am about to start a study of the microbiome in chicken (caecal content) and would like to get an opinion on the available metatranscriptomics rRNA depletion kits. I know that there are several that seem to work well for mammals but I do not know if they perform as well in other animal models.
Thank you in advance.
Tatiana
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thanks to Singh
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Chicken egg yolk is widely used in the cryopreservation experiment of mammalian sperm, especially bull, as a cryoprotectant. Regarding that, I read several publications that use egg yolk as a cryoprotectant on cryopreservation of chicken sperm. Given that egg yolk in chicken is homologous to oocytes in mammals, could this method be justified? Aren't the sperm will undergo a premature acrosomal reaction when mixed with egg yolk?
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Avian sperm would not normally interact with egg yolk in the form you are referring to. To get to the site of fertilization, they must get through the perivitelline membrane to the germinal disk. The yellow yolk material lies beneath the germinal disk, so the sperm will not come into direct contact with it.
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Greetings,
I have listed factors of each animal (mammals) species rescued during a dam inundation such as "can swim/unable to swim", "arboreal/terrestrial", "cryptic/none cryptic", frequency of capture: "common/rare" etc. What analysis can be done in order to determine the contributing factors and grouping of characteristics of the animals that influences it to be rescued? I've been suggested Principle Component Analysis (PCA) but I'm also exploring other options out there.
Thank you.
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Hello Nur,
If your sample is only comprised of rescued animals, then you won't be able to pinpoint features/attributes that distinguish them from non-rescued animals (especially if the vital traits differ by species). At best, you could look for traits that appear in a high fraction of the rescued animals, and propose that these might be salient features for survival via rescue.
Good luck with your work.
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I am looking for research covering the morphology of human lingual papillae. Areas such as dimensions, density, distribution, histology, ultrastructure, diversity. Really this whole, seemingly under represented area of research. I see numerous papers dealing with various mammals but none specific to the human lingual surface.
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Dear Craig try these ones and their references and articles that cite these ones:
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I have inventory data set of vascular plants using systematic distribution and two data sets for large mammal species, 1. camera trap data set and 2. rangers' SMART patrol data set. My objectives are;
1. to find relationship between species richness of vascular plants and large mammals
2. to compare distribution of 3 herbivores in different species richness of vascular plants and forest composition (structure, density, canopy coverage, etc.)
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Hi Myo Min Latt,
the articles below can help (I recommend based on their title and keywords)
Clegg, BW; O'Connor, TG; Manson, AD. (2021) Vegetation classification for the management of large mammalian herbivores: a case study at Mushingashi Conservancy, Central Province, Zambia. AFRICAN JOURNAL OF RANGE & FORAGE SCIENCE DOI: 10.2989/10220119.2020.1827455
Hempson, GP; Archibald, S; Bond, WJ (2015) A continent-wide assessment of the form and intensity of large mammal herbivory in Africa. SCIENCE Vol.: 350, Issue: 6264, pp: 1056-1061. DOI: 10.1126/science.aac7978
Alves, TR; Fonseca, RCB; Engel, VL. (2012) Medium and large sized mammalians and their relation to habitat patches at the Botucatu cuesta, state of Sao Paulo, Brazil. IHERINGIA SERIE ZOOLOGIA 102(2): 150-158.
DOI: 10.1590/S0073-47212012000200006
Reed, KE. (1998) Using large mammal communities to examine ecological and taxonomic structure and predict vegetation in extant and extinct assemblages. PALEOBIOLOGY 24(3): 384-408.
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As I have started a new research work, I am looking into the circadian rythm and genes that controll it in mammal models. In order to do this experimentally I need to find consistent molecular indicators for when the Night and Day genes are activated respectively.
Which would you consider relevant?
Do you know about any molecular markers in bronchial cell lines that indicate the expression of Night/Day genes?
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Dear Daniel Garcia sir, Per (Period) and Cryptochrome (Cry) genes are responsible to maintain the circadian rhythm of mammals. (3 Per genes- Per1,2,3 and 2 Cry genes- Cry1,2). During the day, these genes are activated after the binding of BMAL+CLOCK combined activators to the promoter region and produce Per and Cry proteins then translocate into the cytoplasm. Those proteins can't exist alone therefore have to be made dimers per-per or per-cry dimers. Another molecule/CKε1 enzyme plays a key role in manipulating these dimers. It has 3 functions
1 Phosphorylation of alone per proteins and degrade them, this helps to reduce the formation/accumulation of per-per and per-cry dimers quickly (maintain a considerable dimer formation rate until night)
2 Translocation of dimers into the nucleus
3 Turn over the inhibitory complex
During the night those dimer negative factors interact with BMAL+CLOCK complex and inactivate the genes
Again at the morning the concentration of inhibitory dimers become lower in the nucleus compared to the BMAL and CLOCK. BMAL+CLOCK bind to the promoter to activate the genes again.
Mutations of CKε1affect on the circadian rhythm for an example poor phosphorylation make quick accumulation of dimers then negative feedback starts earlier....
Activation/Deactivation of these genes affect on the body physiological processes- Hormone secretions, Body Temperature, Sleep wake cycle etc.
Photoreception of our eye sends the signals to the brain. These genes are prominent in some structures(SCN-suprachiasmatic nucleus or nuclei is a tiny region of the brain in the hypothalamus in the brain). recognize day and night then activate or deactivate that genes that genes. send signals to the Pineal gland if it is activated to secrete melatonin.
melatonin is a predetermining molecule of other secretions such as thyroxin hormone (in the metamorphosis/growth)
I think Melatonin, Thyroxin or CKε1 levels can be used as measurements in your research.
Best regards!
Lahiru
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How many average genetic distance values of mtDNA control region Dloop for indicative conspecific populations or valid species in Chiroptera?. Thank you
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Hi Husni,
as far I could see, there is not a "magic number" for Chiroptera. Only three species have records in genbank for the dloop region. Therefore, it's really tricky to get a safe threshold, if we can say that exist such a thing.
I'm personally not a fan of thresholds, but you could try to explore the intraspecific genetic distance of the sequences on the database. It will give you a clue about how much this sequence is instraspecifically polimorphic as well as it should overlap the between species distances.
I should also recommend you to take a look at the coalesce analysis like GMYC or the PTP (Poisson's Process Tree) and their bayesian implementation.
Good luck
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We all know numerous examples for sexual dimorphism as the result of sexual selection. Thinking of mammals and birds, males are very often bigger or more colorful and richly ornamented than females. In some species however, females and males look very much alike, (aside from a minute difference in body size maybe), they are monomorphic. Why is that?
I couldn't find an answer myself in the literature so maybe you can give me a hint!
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Never really thought about it. I guess it implies that sexual selection isn't always the strongest player in the field: in cases with strong predation pressure, natural selection could overshadow any potential sexual selection. For instance, consider Zahavi's handicap principle: females will prefer males with conspicuous traits that reduce male survival chance, because it signals their quality ('they can afford it, so they must be good'). But if predation pressure is very high, males may not be able to afford conspicuousness at all (hence not leaving a lot of room for sexual selection).
Just some first thoughts that crossed my mind, I'm not the expert in this.
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I have been looking at weight values for rodents in the family muridae, specifically subfamilies: gerbillinae and deomyinae. I found some considerable discrepancies in the values for the same species from different references. Generally, I get similar values from sources concerned with African mammals (Mammals of Africa, Kingdon et al, 2013; Mammals of Sub-Saharan Africa, Monadjem et al, 2015; The Complete Book of the Southern African Mammals, Mills and Hes, 1997; The Contemporary Land Mammals of Egypt, Osborn and Helmy, 1982). The values I get from other sources, namely PanTheria, AnAge and Alhajeri et al (2015) are mostly similar amongst themselves but can be very different from those reported in the first (“African”) set of references.
The similarity within set cannot be solely explained as repeated citations from the same old reference; so I was wondering if it can be explained by biogeographic trends within widely distributed species. In other words, the set of references concerned with Africa is reporting species values from African populations only; while the other references report values from the world-wide distribution of the species. The observation that species with African and extra-African populations have wider ranges of values reported in PanTheria, AnAge and al-Hajeri compared to those in “African” sources for the species is consistent with this hypothesis. Furthermore, whenever a species is endemic to Africa, the two sets of references seem to largely agree.
Could somebody please corroborate/debunk this idea of mine, or suggest other explanations for these puzzling discrepancies?
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I would check other values like head-body length to see if there is intrapopulational variation. If the discrepancy is really due to intraspecific variation you would expect linear dimensions to show a similar pattern to body mass, since you would be measuring bigger dimensions on bigger animals.
However, it's also possible that the reported body masses are just plain discrepancies. I've noticed a lot of second-hand databases like PanTheria can be extremely inaccurate when it comes to reporting body mass values because they often just collate them from the previously published literature. A lot of times studies will use ballpark values or the midpoint of guestimated ranges from sources like Walker's Mammals of the World, which aren't very accurate or precise. A good example of this is the South American rodent Dinomys branickii. Most studies using body mass for Dinomys will report a value of 10-15 kg or 13-14 kg, which seems to be drawn from a ballpark range of 10-15 kg mentioned in a paper from the early 20th century (maybe Allen 1942). This doesn't appear to be based on any measured specimens, and no studies list specimens from where these data were collected. However, actual weights of Dinomys based on first-hand measurements of collected wild individuals (e.g., Osbahr et al. 2009, Gottdenker et al. 2001) finds that the body mass of Dinomys is actually only about 9.5 +/- 1.1 kg, a much lower estimate. Larger individuals are known but are highly gravid, captive ones. Nevertheless, people have been reporting a 13-15 kg body mass for Dinomys as if it were gospel.
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Is there evidence of leg-right sidedness in parental genomic imprinting - that is, maternal alleles expressed more on one side of the body of a mammal and more paternal alleles on the other? I have a memory of hearing a report of such years ago, but I can’t find it anywhere in the literature.
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But, that sounds very interesting.
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I have been working on a study trying to estimate body mass in mammals using a skeletal proxy in R. My dataset has high phylogenetic signal (Pagel's Lambda = 0.9) and I am interested in removing the effects of phylogenetic signal to produce more accurate estimates of body mass.
I had tried to do this using PGLS, but PGLS ended up producing higher error rates than the same data under OLS despite the high phylogenetic signal. Part of this appears to be because there are several particular clades with long branches (e.g., Monotremata) that form regression lines above or below the line of best fit, and their phylogenetic position distorts the resulting best-fit line. I had thought PGLS took phylogenetic covariance into account when estimating values, but it looks as though it only uses phylogenetic signal into account to minimize the residuals of the best fit line. It does not put the phylogenetic covariance back into the estimation of the y-value.
I.e., the function does not go: "Taxon X is positioned as sister to Monotremata. Therefore it should deviate above or below the regression line to a degree similar to its phylogenetic distance and the mass estimate should be adjusted accordingly?"
Given this, is there any way to use a topology to estimate a value that considers phylogenetic covariance and the phylogenetic postion of the unknown taxon when estimating this value, rather than just using it to minimize the residuals when calculating the best fit line?
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Agreed. PVR R-package is all you need to run your analysis.
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Hi all,
I am currently looking into the prevalence and effects of arthropods as predators of vertebrate brood. To do so, I'm building a database consisting of observations of arthropods predating vertebrate young that are dependent on parental care, in terrestrial systems (think for example of nestlings in birds or young mammals).
So if you, during your field work or in your free time, have observed such a predation event (or know anyone who has), please share it with me with as much detail as possible!
Thanks in advance for your help
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I have observed an attack by a scolopendra (Scolopendra cingulata) on young reptiles. They also attack chicks of birds nesting on the ground. These observations are in the Astrakhan region, Kalmykia, Stavropol Territory, Crimea and Kazakhstan.
Regards, Sergey
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I've been looking for a test to compare vertical stratification of mammals and none seem to fit my desire. I have two strata in the canopy and my objective is to see if the arboreal mammals use these strata differently.
I don't know the abundance of these species, but through sampling I have N of events of each species on each strata. Can I use the B-C dissimilarity to compare these strata?
If not, is there another test you'd recommend?
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In this study case, i think the correct index applied is Similarity of Sorensen.
I give you some papers can help you for your request:
  • Balmer, O. (2002). Species lists in ecology and conservation: abundances matter. Conservation Biology, 16(4), 1160-1161.
  • Chao, A., Chazdon, R. L., Colwell, R. K., & Shen, T. J. (2005). A new statistical approach for assessing similarity of species composition with incidence and abundance data. Ecology letters, 8(2), 148-159.
  • Santini, L., Belmaker, J., Costello, M. J., Pereira, H. M., Rossberg, A. G., Schipper, A. M., ... & Rondinini, C. (2017). Assessing the suitability of diversity metrics to detect biodiversity change. Biological Conservation, 213, 341-350.
Good luck,
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I would like to know some methodologies for captive condition of small carnivores in Zoos. … Read more
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You must develope your aim in this study in order to define methodology.
I invite you to see this link : Gidna, A., Yravedra, J., & Domínguez-Rodrigo, M. (2013). A cautionary note on the use of captive carnivores to model wild predator behavior: a comparison of bone modification patterns on long bones by captive and wild lions. Journal of Archaeological Science, 40(4), 1903-1910.
Benson-Amram, S., Gilfillan, G., & McComb, K. (2018). Numerical assessment in the wild: insights from social carnivores. Philosophical Transactions of the Royal Society B: Biological Sciences, 373(1740), 20160508.
or this :
Ferreira, S. C. M., Torelli, F., Klein, S., Fyumagwa, R., Karesh, W. B., Hofer, H., ... & East, M. L. (2019). Evidence of high exposure to Toxoplasma gondii in free-ranging and captive African carnivores. International Journal for Parasitology: Parasites and Wildlife, 8, 111-117.
Good luck,
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I would like to know some methodologies for captive condition of small carnivores (mammal) in Zoological gardens
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I think that zoological camera Can help you. So, you Can observed species indirectly. For the sampling, you must tell us your aims ?
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I read a few articles related infant & dog's auditory preference. They both mentioned "baby talk" could be more attractive to infant & dog. And my team and I want to find out the reason from evo-psych. We already know one of necessary condition is high pitch, and had some data about high pitch preference for dogs (we took gaze duration to be parameter).
Our project focus on evo-psych puzzle, so we want to exclude the physical or physiological reason to explain why dog prefer high pitch. I tried to do some reviews to know physical trait of high pitch. But I almost found nothing help because I barely know the domain knowledge of acoustics. So if physical trait of high pitch could explain why mammal like human and dog has such preference, then we should reschedule our project.
Thank you for response such fundamental question.
Sincerely, Jhou.
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Higher sounds (including out of the auditory range of humans) are also emitted by small prey animals which may supplement scavenging in feral dogs and engage prey drive even in companion dogs and those that have had prey-seeking traits bred out. They seem to engage the attention of most dogs which is why squeaky toys work.
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I m very interested in Paleogene continental north Africa, manily because of the "strange position" of Laurasiatherian mammals living there that times.
But I have not found many things about paleogeography constraints.
Can you suggest me one or more articles about this topic?
Thank you
Aldo
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thank you
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Very basic stats question, I have a measure of the % females breeding each year within one mammal population and I want to test between the years. Firstly, what is the best stats for this kind of analysis? Secondly, I'm hoping to then incorporate aditional categories into the test, i.e. Total young born etc. How will this change the test?
My data looks like this:
Yr 1 Yr 2 Yr 3 Yr 4 Yr 5
% breeding 80% 60% 70% 40% 90%
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Hi, depending on your number of data points per year, you should use 1 way ANOVA. I agree that you should take a look at the normality of your distribution (I usually go with Shapiro-Wilk unless my n is very big). When you add components to it you can do 2 way anovas followed by Tukey's posy hoc test. I would advise against doing t tests being 2 given years, this is what post-hoc multiple comparisons are for.
Best,
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Recent, I had read one paper :https://academic.oup.com/nar/article/40/22/11463/1147721. In this paper, the author identified hundreds of conserved non-coding genomic regions that were independently lost in mammals. It is very interesting. But, I want to know how do extract hundreds of CNEs (conserved non-coding element) loss events from whole-genome multiple alignment files (MAF) in specific-lineage.
Is there have some software or scripts can do it.
Thanks.
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What are the best thermal imagers to use in the study of insectivores, rodents and carnivorous mammals in soil horizons? The ability to detect herpetocomplex with a thermal imager in natural shelters?
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@Bara Mouslim
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A new study that researchers from Tel Aviv University in Israel: They scanned the brains of more than 120 different mammals and found that brain connectivity is neither higher in humans nor dependent on the size of the brain.
“Many scientists have assumed that connectivity in the human brain is significantly higher compared to other animals, as a possible explanation for the superior functioning of the ‘human animal,'” explains the study’s first author, Prof. Yaniv Assaf. To test this assumption, Prof. Assaf and his team used a type of brain scan called diffusion MRI to scan the brains of 123 different species of mammal, including humans. It was the first time that researchers had placed the majority of these animals’ brains inside an MRI scanner.
“ the brains of all mammals, from tiny mice through humans to large bulls and dolphins, exhibit equal connectivity, and information travels with the same efficiency within them,” explains Prof. Assaf.
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Yes, I think so.
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I´m looking for papers showing raw abundance data for different groups of animals, plants, and bacteria. I would be interested in studies on: mammals, birds, reptiles, amphibians, insects, fishes, annual plants and trees.
Any recommended paper will be of great use.
Thank you so much !
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The ATLANTIC and NEOTROPICAL series data papers published in Ecology has data about many groups....see https://esajournals.onlinelibrary.wiley.com/doi/toc/10.1002/(ISSN)1939-9170.AtlanticPapers
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I'm looking for datasets (or databases) in which the body mass of predator and prey are presented. I'm specifically interested in terrestrial predators (snakes, mammals, birds).
An example of is Vézina, A.F., 1985. Empirical relationships between predator and prey size among terrestrial vertebrate predators. Oecologia, 67(4), pp.555-565. (unfortunately there are no associated data as it's quite an old paper).
Any tips or suggestions are more than welcome!
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Giulia Masoero Jerry Olsen thanks a lot! I will include these in my data set!
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I am slowly putting together a very basic identification key of plant damages, to determine first if the source of the damage is living or not, and then what did it: bacteria, fungi, insects, mite, small/medium/large mammals... I was quite surprise at the first place to not find such material already produce.
I hope this material to be used by parataxonomists in Central Africa. It should therefore be accessible and well-illustrated. It also do not need to go too much into details: for example, to conclude the key at 'Insect' is fine, we do not need to know which Order of Insects did it.
I have seen quite some apps and other websites for cultivated plants, but none are quite as simple and general as what I am looking for. Maybe I was simply looking at the wrong places.
Any suggestion is welcome. Thanks.
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There are many species specific manuals and Compendia, such as APS Press:
Some tools:
Regards
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For an upcoming book (in danish) on managament of forest biodiveistty, I would like to present a table summarizing the biodiversity value of various woody species. I wonder if there are any databases or other overviews avialable that gives insight into which species of mammals and birds that (regularly) eats the seeds from various tree genera/species. Help with be appreciated
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Thank you for sharing your knowledge
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While working on mammals, we recorded vocalisations (i.e. clicks) that could possibly be explained by echolocation. What would be the most straightforward way to test whether an animal uses echolocation? If we record the sound, can the sound structure provide any evidence? Would the ears or other physiological features of the animal provide any indication? What would be the best, straightforward approach to provide us with some evidence?
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We are licensed to carry out bat activities (husband) and bat caring (both of us), so are familiar with echolocation and bats. The bats in the UK that we deal with mainly have, within their pinna is a small structure called a tragus which helps the bat to receive sound. Horseshoe bats have no tragus, but their antitragus is well developed. We identify bats by picking up their echolocation frequencies using a bat detector; different species use different frequencies. Perhaps you could use a piece of equipment like a bat detector?
These are interesting links:
This is more light hearted:
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Hello all,
I am trying to obtain information about the specific conditions for the method of preserving feces samples for mammals ( preservative type, preservative concentrate, preservation temperature, ....) in order to perform a phylogenetic analysis.
Thank you
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Thank you so much for this response
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Hi everyone!
In what free journal can I publish studies of large mammals activity patterns???, both in English and Spanish...
Thanks,
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Mammal Review - Wiley Online Library
onlinelibrary.wiley.com › journal2019 Journal Citation Reports (Clarivate Analytics): 50/168 (Ecology)8/168 (Zoology) ... Mammal Review publishes five article types: Reviews (new analyses of published ... and Comments (responses to papers published in Mammal Review). ... rapidly than in the last 20 years, and further large increases can be expected.
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I'm in the process of buying new equipment to record the vocalizations of wild mammals, and have used Marantz's solid state recorders with great success in the past. However, it seems that their PMD range of recorders are not easily available anymore. I've looked around and the Tascam DR-1 portable solid state recorder seems like a good option, but I have no experience with that product.
I'd appreciate any advice on the best, portable recorders to be used in the field. Nothing too fancy (I don't expect ultrasonic vocalizations, for example), but I'd love to hear from you about the pro's and cons of different recording tools that you've used. I would need the recordings to be of good enough quality to analyze properly in programs like AviSoft.
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Hi Alina,
I have used many recorders throughout my career and it wasn't until I discovered the Sound Devices Model 722 that I stopped buying other field recorders. It is a two-channel digital recorder (they also make a 4-channel model), made in the US, with a wide, flat frequency response that enables you to record up to the high-frequency limit of your microphone. So if we want to record ultrasound, we use an ultrasonic microphone. If we don't need this capability, we use a standard directional mike that drops off at 18 kHz or so. The microphone signal is digitized at a sampling rate selected by the user via a menu. The digitized signal is stored as a .wav file on the internal compact flash card. When this is filled, you can easily transfer the contents of the CF card to the internal 40 GByte hard drive. This is a small, lightweight recorder that can run on rechargeabale batteries that give a recording time of 6-12 h. Take a look at their website- I think you will be pleased. By the way, I have no connection to this company!
Hope this helps-
Peter
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I think if Akkermansia muciniphila has been found in breast milk, it should also be present in the milk of other mammals. Did anyone find it in raw cow's milk?
In what other foods was A. muciniphila searched / found?
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Triggering Akkermansia with dietary polyphenols: A new weapon to ...
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Dear RG community,
I am looking at the limits of ACTIVE flight of various animals.
I am only interested in animals that are capable of active flight. Active flight (also called powered flight) is a type of animal flight that uses muscles to generate aerodynamic force that is sufficient to generate enough lift and thrust. So no flying fish, no frogs that jump from the treetops and use membranes between their fingers to controllably parachute and fall-down, and no flying squirrels etc. Such examples do not count as active flight under the above definition of active flight.
I am interested in limits of ACTIVE flight of the following animal groups:
a) insects
b) birds
c) reptiles (extinct, like pterosaurus)
d) mammals
I am particularly interested in the data on the following:
1). Maximum Altitude (km) a given flying animal was observed
2). Longest Travel Distance (km) - I intend it to be only continuous flight, from initial take off to landing (i.e. without landing and resuming flight afterwards for the second time), otherwise a lot of organisms essentially have no limit to distance.
3). Maximum Speed Observed (but it is less critical)
If any of you know papers or studies that show such limits for example organisms of insects, birds, extinct reptiles and mammals please let me know.
Thank you very much for your help, time and consideration.
JJ
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Our group (we are zoologists focusing on animal orientation) is considering buying a georadar to map the tunnels of subterranean mammals. We would like to study the position and direction of the tunnel networks of animals of various sizes (from voles to bigger animals such as ground squirrels, i.e., tunnels with various diameter in various depths) living in various types of soils and environments. When we received the visualization of our trial measurement of voles tunnels on the field, we were, however, a bit sceptical whether this is a suitable method (difficult to discern what is a tunnel and what some other anomaly such as rocks etc.).
  1. Any tips, tricks or experience with using georadar in animal ecology?
  2. How to get the most precise visualization of the networks?
  3. Would you recommend to use the 750 or 450 mHz antenna?
Thanks!
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Thanks, Niklas! That is indeed really nice and thoroughly conducted study! :) I am already in contact with Adam Booth.
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Hi!
Is there another way to measure the relative brain size of a mammal specie than the encephalization quotient (EQ)? Is appropiate to use the EQ as a measure?
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thank you all!!!
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I am conducting a study using wildlife traits, however, home range data is limited.
Pd. I have already used data from Tamburello et al. 2015, but I still have lots of species without home range data.
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COVID-19 has been found in mammals in the wild, and in domesticated mammals. What about birds?
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There is no any concrete scientific evidence to prove that birds can host COVID-19.
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I am searching an advice on how long a tick could be find always sticked (not moving on freely) on an animal after its dead?
I think that this is depending of the age of the tick, and the volume of blood they are still in need.
But is it possible to find some ticks still hooked after 3 days, 7 days, Less or more???
I wonder this question especially for big mammals like elephant, but data from other taxa are welcome
All papers or experiemental data are welcome.
Thank you for your suggestions
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few hours after his death
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- Therefore, I would like to know if anyone has carried out tests since these waves are capable of interacting in the respiratory passages of exposed mammals.
- Also taking into account and without ruling out the digestive system, as a possibility of testing.
Or if someone can think of how to safely perform that test.
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almost all the articles that I have put are open. Some others had to ask the authors for permission.
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Dear all,
I am looking for a commonly used Mammalia nomenclature resource/database/catalog. Preferably an online resource that is continuously updated. Similar to Frost (Amphibia) or the HBW (Aves).
I found the Mammal Diversity Database (https://mammaldiversity.org), however as I'm not a mammologist I wanted to make sure that I'm not missing other resources that are commonly used.
Thanks in advance.
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Hi.
About the taxonomy and nomenclature of mammals of Italy (and by extension of many countries of Europe), you can see two recent publications with different views about the topic (attached here).
Best regards.
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I am conducting some seed predation research and want a rough estimation of the local abundance of mammals. I have been suggested that scat counting is a relatively easy way to obtain such an approximation.
I have already identified the paper by Birks et al. (ARE SCAT SURVEYS A RELIABLE METHOD FOR ASSESSING DISTRIBUTION AND POPULATION STATUS OF PINE MARTINS) but I was wondering if anyone knew of any studies that used this method?
What about the use of plots to determine the density of scats?
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It is also my question. How I can determine individual species density from scat survey?
I am conducting research on carnivore biodiversity.
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Hello Community,
I'm compiling trait information of vertebrates and I was wonder if anyone knows about good papers or other types of documents where I can find information related to the dispersal distances, capabilities or proxies to these two measures. I know that for mammals and birds there is information available but reptiles, like usual, seems to be a little bit neglected in this aspect. Any helo will be more than welcome :)
Thanks,
Gonzalo
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This can be a rather complicated subject and variations will occur with the same species within different geographical ranges. Habitat, length of active seasons, species, age, and sex will also will play a role in sizes of home ranges. The majority of my work is with timber rattlesnakes in middle Tennessee in the USA. I am attacching a PDF of my dissertation that can provide information on movements of this species. I hope it can provide some insight to your question.
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Hi All, we have a bioinformatics challenge and we would love any help this community can offer. We have data from a target-enrichment experiment that was supposed to capture certain microsatellite motifs. The three enriched libraries were sequenced in a rapid run on Illumina Hiseq 2500 (paired end mode) and our data is in the standard illumina fastq output. Our three libraries come from three different sources. The first library is developed from fresh fish tissue; the second one is mammal tissue; and the third one is the same mammal species but from fecal samples. For the fecal samples, we need to somehow filter out sequences belonging to the mammal only (i.e. not prey or microbiome). We have a reference genome for the mammal, but not for the fish. The data has been demultiplexed already (so for the fish we have 40 individual fish each with its own .fq file containing all the read data). Now, we are facing the challenge of how to deal with this data. Although we are familiar with most basic bioinformatic tools and analyses we do not have advanced programming skills. We need to find a way not only to find and identify the length of our microsats within the reads but also (for the fecal library) somehow be able to identify unique flanking sequences that would correspond to our mammal, in such a way that the reads of other species in the fecal libraries can be excluded. Would anyone have a suggestion on what approach(es) we could use? We have already (unsuccesfully) attempted to tackle this with SSR_pipeline. Thank you in advance for any help you can offer - it is very much appreciated! Daniel & Vania
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Vania Carolina Fonseca da Silva You may want to consider a pangenomic approach whereby you use K-mer count sets with calculable probabilities of a faecal K-mer set originating from a fish species or group of species.
Because you have sampled enrichment you only have a probability of sampling any given SSRs in each species, and it is difficult to determine that probability for each fish species if the genome is unknown.
My suggestion is to take each set of fish reads and do a K-mer analysis, maximising the K-mer size until you obtain a distribution such that – guestimate – 10% of K-mers have coverage of between 10 and 100 copies
The guestimate of 10 is because you are expecting some coverage at the sampled SSRs and less than 10 could be indicative of sequencing errors. My guestimate of 100 is because you are looking to discard the SSR repeat polymerics and are looking for K-mers likely to be unique both intra and inter fish species. Hopefully for each fish species you will end up with many thousands of identified K-mers accepted within the copy number constraints and of maximal size up around 50% of your read lengths. You can’t use K-mer sizes up around read lengths simply because most K-mers will be discoverable through partial read overlaps, not full length overlaps.
Repeat iteratively over all fish species, adjusting the K-mer size until some minimum number of K-mers per species is reached, and build a matrix containing columns: fish species, K-mer sequence, count of that K-mer present in faecal sample reads – initialise count to 0.
With your faecal sample reads use a sliding window of the K-mer size you built the final accepted matrix with, and slide this window along each read.
For each K-mer from the sliding window check if that K-mer is present in the mammal genome – if so then discard and try next window.
If K-mer not in mammal genome then search matrix for a matching K-mer sequence and for every fish which matches then increment that fishes faecal K-mer match count.
When all K-mers in the faecal sample reads have been processed you can process the counts in the matrix and assign probabilities of the mammals individual fish species (or groups of fish) consumption.
I suggest that you discard counts of less than some threshold – say 10 - as these could represent sequencing errors.
The foregoing approach will need much optimisation to become practical, but could be applied even when the prey consumers genome has yet to be assembled providing you have reads from the consumer species. K-mers from the consumer reads are added to the matrix and so if count more than some threshold then discard that K-mer from further analysis!
I am interested in your research and may be able to provide some bioinformatics expertise ( https://github.com/kit4b ), do you have further details of your experiment you can share?
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In his 2007 book Chasing Kangaroos, Tim Flannery rather extensively discusses the idea (treated in the text as something supported by scientific research, rather than an untested hypothesis) that the primary symbionts involved in foregut fermentation in kangaroos are strongyle nematodes, rather than single-celled microorganisms. I have been trying to look up the origin of these observations in the scientific literature, but have not been able to find any papers regarding this. Not even any papers “this was a hypothesis that was once suggested but has since been disproven”. All of the papers I have seen regard strongyle nematodes as parasites. The book itself does not cite any specific literature for this observation, and the bibliography does not mention anything about further references on kangaroo fermentation.
I was wondering if anyone knew the origins of this observation, and if it has been supported by the latest research.
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It can be rather challenging to find all bibliographical sources, some obscure / omitted ones will appear time to time. Tim Flannery is responsive colleague, why not to ask him directly?
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Hi everyone,
I am thinking about using JSDM for small mammals communities in Europe following HMSC package. When performing these kind of analyses for birds, I obtained the phylogenetic correlation matrix from http://birdtree.org/. However, in http://vertlife.org/ it seems that mammal phylogenetic subsets are not available for now. Is there any other source where I can find this kind of information?
thanks!
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Thanks! I will check it!
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A study was conducted on a kind of endemic marine mammal in a land locked sea. The coastal line is about 1000 km from West to East. 20 microsatellite markers had used and the results of DPAC analysis using adegenet (R package) Fig 1 and STRUCTURE software (1000000 MCMC, 10% burnin) Fig 2 were shown two different populations of this species. The results reinforce the hypothesis that there is more than one population in this closed sea. Now, I've three questions here:
a) Is there any possibility to have two populations for mentioned species in a land locked sea with 400000 km2 area?
b) With these results, can we conclude that there was a gene influence form the east population to the west population?
c) How about interpretation for DAPC graph with two curves?
P.S: In both barplots, red cluster represents west population individuals, while green cluster indicates East population individuals.
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Hmm, without knowing the species and a bit about its distribution/dispersal ability it is hard to say. What you are showing could be the result of 2 diverging populations, or it could be the result of sampling 2 locations within a single population that are far-enough apart that isolation by distance accounts for the depicted differences. If the "populations" are discontinuous, I then begin to wonder if this is a natural process, or a relic of anthropogenic pressures (hunting, etc) fragmenting a once-continuous population - then drift, etc between the two "populations" resulting in the observed patterns. I'm curious what the pairwise FST, expected heterozygosity, and observed heterozygosity are? These should prove useful in determining down-stream analyses. Also, I assume no complimentary mitochondrial or SNP data is available?
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I have been looking for a comprehensive review that compares African grasses, both cultivated and wild in terms of productivity and feed quality for livestock. There are some studies looking at grasses in national parks and the intake by wild mammals. I wonder whether somebody evaluated the literature for farm animals (large and small ruminants). I would appreciate suggestions and links to resources. Ideally, we build a database with this.
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I am conducting research in which I would like to see if I can detect a threshold response in mammal species richness in relation to the surrounding level of forest across study sites. I plan to use multi-species occupancy modelling methods using a bayesian approach in R to determine species richness in relation to the forest cover covariate, but I am a bit stuck on how I would then proceed to determine break points/thresholds in the species richness across sites in relation to forest cover. I have done a bit of a research into methods/tools for determining breakpoints (such as Muggeo's segmented package in R) however I am wondering if anyone has suggestions for how I could both use these multi-species occupancy modelling methods to determine species richness in relation to the forest cover covariate, and also determine if there is a threshold relationship between species richness and forest cover in any sites using tools in R? Thanks very much!
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Instead of threshholds, you can compare pristine areas with disturbed
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Hi all, I've got a question with FDis values from 9 mammal assemblages which range from 4.001 to 5.031 (mean ± SD = 4.690 ± 0.290). I'm using a set of 28 traits, mostly binary traits. As far as I know, these values appear to be very high. For the same assemblages, functional evenness ranged from 0.481 to 0.667 (mean ± SD = 0.557 ± 0.048) and Rao’s square entropy from 17.62 to 26.04 (mean ± SD = 23.150 ± 2.582).
Thank you in advance for any suggestions!
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I calculated FDis several times and there are often values that exceed 1. I don't see the need for constraining the values between 0 and 1.
The function dbFD uses principal coordinate analysis to construct the multidimensional trait space. If there are categorical variables, PCoA is based on the Gower dissimlarity matrix. I don't think it matters much whether you include those traits as binary or 1 categorical variable. You can compare the two and let us know.
The number of traits per se is not a problem. As mentioned above, you can include a large number of traits. However, you should only include the traits that are relevant for your research question (eg if you study the relationship between FD and a certain ecosystem function, you should only include the traits that are revelant for that ecosystem function. And you should not include all traits you have).
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Hi,
I am trying to do research into new ways scientists are researching and understanding emotions in animals, (mammals, fish, birds, insects). Would anyone know of any interesting papers on the subject?
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Please also have a look at these useful RG links and a PDF attachment.
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Hi, I am planning to set up a long term monitoring study using camera traps in Peru. In terms of choosing station location, number of cameras, distance within stations and orientation of the camera, could you offer any advice? Is there any paper you have used in