Malarial Cell Culture - Science topic

Please take a look at this useful link.

These are not strange nucleotides, but they represent ambiguous peaks at those positions, and polymorphism. Each of these letter specific combinations of signals and are defined in the IUPAC list of ambiguous nucleotides. You need to check these positions in your sequence electrpherograms and confirm. If you have peaks of comparable intensity, you may retain these letters while submitting your sequences to the GenBank. GenBank accept sequences with IUPAC codes. Fllow the link while revising your sequences:

SD

you can contact https://www.researchgate.net/profile/Gordon_Awandare

I do not know your application but I have used the trehalose based freezing solutions with human epithelial cells.  We thawed the cells the same way with any cryo-media, Fast.  Threhalose is not considered toxic.  Similar to raffinose or other branched carbohydrates.

General procedure:

1. Vials were swirled by hand in a 38 C water bath. 

2.  As soon as the lat remnant of ice disappears, we very gently diluted the crryo-media 10 fold

3. cells allowed the cells to rest for 30mins-1 hour in aerobic conditions. 

The thaw is the highest stress point for cells in the cryopreservation procedure.  Respiration restarts and membranes have to be repaired.  Between the damaged membranes and the superoxide generation, not all cells will make it out of cryo.

Hope this helps

Hello Baraka,

The best way u can go about it is what Lamoussa had adviced. 

Regional Público Health Centre; before as Malaria Research Center. Contact Ildefonso Fernandez: ifernand1@hotmail.com