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Maize - Science topic

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Some maize plants will turn purple. Even reaching 100% purple leaves yet the growth remains normal. The grain yield and cob size remain normal. Soil analysis is ok and Nutrient analysis in the maize is also ok. This phenomenon confuses farmers as they think it is a disease.
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Purple Maize Zea Mays Corn. Purple of the corn plant is turning out to be one of the healthiest foods on the planet. It is loaded with phenolics and anthocyanin and has an antioxidant rating higher than. blueberries. The health benefits of purple corn are pervasive in the body, and it has a normalizing.
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What specific natural plant nutrient sources or plant growth-promoting sources, such as BIOSTIMULANTS, BIOFERTILIZERS, etc., would you use for starting cultivating tropical crops like corn, sorghum, millet, peanuts, tomatoes, and onions in a middle scale production in a tropical country as Simbabwe, where chemical fertilizers are economically not afordable or either unavailable, but where some animal dung is accessible?
How economically successful is it which commercially available mycorrhiza to use or other microorganisms of the soil microbiome with similar benefits such as PGPR (plant growth-promoting rhizobacteria), PGPF (plant growth promoting fungi), PGPM (plant-growth-promoting microorganisms), as well to use seaweed, algae stimulants or verimcompost?
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Vermicompost or worm castings are excellent biostimulants.
If leaves are raked around a tree and watered it creates a habitat for worm activity.
The castings which accumulate are wonderful to start plants.
This material can placed in the planting hole.
Worms also appreciate animal bedding with manure.
The wormed and composted material will concentrate the plant trash making its movement more manageable.
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Using which MYCORRHIZA for low cost tropical agriculture? Or other BIOSTIMULANTS, BIOFERTILIZERS?
What specific natural plant nutrient sources or plant growth-promoting sources, such as BIOSTIMULANTS, BIOFERTILIZERS, etc., would you use for starting cultivating tropical crops like corn, sorghum, millet, peanuts, tomatoes, and onions in a middle scale production in a tropical country as Simbabwe, where chemical fertilizers are economically not afordable or either unavailable, but where some animal dung is accessible?
How economically successful is it which commercially available mycorrhiza to use or other microorganisms of the soil microbiome with similar benefits such as PGPR (plant growth-promoting rhizobacteria), PGPF (plant growth promoting fungi), PGPM (plant-growth-promoting microorganisms), as well to use seaweed, algae stimulants or verimcompost?
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Go to the Rodale Institute website Douds and the collaborators have formulated the method for on farm mycorrhizae production.
The on farm propagation gives an adaptive mixture of species with ability to be effective under your conditions.
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I am a very new researcher in science and technology. Currently doing some research using machine learning, deep learning. I have publish one research in Agriculture Engineering using deep learning and my thirst is growing. That's why I am looking for collaboration with someone to start work more advance and want to design a virtual research lab. Here anyone can join as a research collaborator just contact me on my email Mail: 20220901004@stumail.sdut.edu.cn
Address: Shandong University of Technology, Zibo, China
Wechat: Haq1133
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Good morning
we are only monitoring the physical variables, we have not developed a model for the detection of pests
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Hello everyone
as we know single crosses are known for highest heterosis in hybrid production method compared to their parents (inbred lines). but what has been happened to this F1 progeny (for example in maize) if they appeared weak in field (low germination, emergence, height, etc.) and the performance is not acceptable?
I'll appreciate all of your helps.
Bests,
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Dear ALex,
thank you so much for your useful and interesting answer.
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In Kg per hactare
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Depends on the quality of nutrients in the FYM and recommended nutrient requirement of the maize variety as well as soil nutrient status. Another important factor when calculating the amount of FYM is the moisture status of the organic material/FYM
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We use PVP (Polyvinylpyrrolidine) for our maize leaf DNA isolation protocol. The molecular weight of our PVP is 40,000. A collaborator shared their protocol, and it is very similar, but uses PVP with a molecular weight of 360,000.
What is the difference? Does one remove phenolic compounds better?
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I have a recipe for a flow cytometry buffer that uses 4% PVP-10. What does this mean? Is it PVP with a Mw of 10,000?
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Hello dear researchers.
I need a favor let me know about seed density and how to measure the seed density? is there any instrument? I have 900 seeds of each line and overall lines are 50 maize natural population.
Thanks for valuable answer.
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Paul Reed Hepperly i have overall 900 seeds of each line and first weight of 900 seeds or 100 seeds ?
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My plant samples are Arabidopsis and maize
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Thanks for your input
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How can maize be integrated with other crops and livestock in sustainable and diversified farming systems?
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You can integrate corn and legumes(like cowpea as a living mulch) by intercropping them in a best spatial pattern to increase forage yield and weed suppression, like what I did in my article:
10.22092/IJWS.2022.356354.1400
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Our research article Title: Assessing Impact of Climate Variability and Change on Maize Yield in Gamo Zone, Southern Ethiopia: A Modelling Perspective. Authors Gizachew Kassa, Manyazwal Getachew, and Anirudh Bhowmick
appears in your web site. Please remove it. It creating hindrance in publication.
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I did not put it on my ResearchGate pages. But ResearchGate does sometimes put relevent papers for a certain topic as an annex to one of my papers.
Please ask ReserachGate to remove it if it is hindering you.
Best regards and success,
Frank
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Genetic and Breeding (Maize)
Plant Breeding it's my DNA
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High-throughput phenotyping for cob damage in crops involves the use of automated and high-throughput imaging and analysis techniques to rapidly and accurately quantify the extent and severity of cob damage in a large number of plants.
One approach to high-throughput phenotyping for cob damage is to use imaging technologies such as visible and near-infrared spectroscopy, hyperspectral imaging, and thermal imaging. These technologies can capture detailed images of the plants and cob structures, which can be analyzed using computer algorithms to detect and quantify the extent of damage.
Another approach is to use sensors and other monitoring devices to track the growth and development of plants over time, and to detect any changes in cob morphology or other physical characteristics that may indicate damage. This approach may involve the use of non-destructive imaging techniques, such as X-ray computed tomography (CT), to visualize the internal structures of the cob and identify any signs of damage or disease.
High-throughput phenotyping for cob damage can also involve the use of machine learning algorithms and other data analytics tools to identify patterns and trends in the data collected from multiple plants. These tools can help to identify key variables that are associated with cob damage, and to develop predictive models that can be used to identify plants that are at risk of developing damage in the future.
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Poultry droppings for maize
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The recommended rate of poultry droppings for maize can vary depending on several factors such as soil type, nutrient content of the poultry droppings, climate, and maize variety. In general, poultry droppings are a good source of nitrogen, phosphorus, and potassium, which are important nutrients for plant growth.
According to some studies, the recommended rate of poultry droppings for maize can range from 2 to 6 tons per hectare (or about 0.8 to 2.4 tons per acre), depending on the nutrient content of the droppings and the soil fertility. It is important to note that applying too much poultry droppings can result in nutrient imbalance or toxicity, which can negatively affect plant growth and yield.
It is recommended to conduct a soil test to determine the nutrient content of the soil and the appropriate rate of poultry droppings needed for maize production. Additionally, it is important to apply the poultry droppings at the right time and in the right manner to maximize their benefits and minimize their negative impacts.
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I am talking with reference to the MLO gene family as in Barley where it gives resistance to powdery mildew.
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The MLO (Mildew resistance locus O) gene family in plants is known to play a role in resistance to powdery mildew diseases. While it is true that powdery mildew does not infect maize, the MLO gene family has been found to be involved in various biological processes in maize, including seed development, stress response, and plant architecture.
Several studies have suggested that the MLO gene family in maize plays a role in plant defense against other pathogens and pests. For example, one study found that the downregulation of MLO genes in maize increased the plant's resistance to the fungal pathogen Fusarium verticillioides, which causes ear rot disease in maize. Another study found that the overexpression of MLO genes in maize increased the susceptibility of the plant to herbivorous insects.
Therefore, while powdery mildew may not infect maize, the MLO gene family in maize could still play a role in the plant's response to other pathogens and pests. Further research is needed to fully understand the role of the MLO gene family in maize and how it could be utilized in crop improvement programs.
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I need to apply microbial strains/inoculant to maize seeds, but I want to know that whether seed inoculation is better or seed-bed inoculation application will be better ? And also, please tell me that how much amount of strains will I required to apply for seed inoculation as well as for seed bed inoculation? Looking for your valuable suggestions.....
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The amount of microbial strain or inoculant needed for seed inoculation depends on several factors, including the type of microorganism, the target crop, and the desired outcome.
In general, a higher inoculum density is required for microorganisms that have a lower survival rate or a lower competitive ability in the soil.
It is also important to consider the dilution effect of the inoculant when it is mixed with the substrate or soil.
The optimal inoculum density for seed inoculation can be determined through field trials and experimentation. It is recommended to follow the manufacturer's instructions for the specific inoculant being used and to adjust the application rate based on the results of previous trials.
Thats what i think .....
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please recommended me How much seeds are required for each replications? and which is suitable methods in which condition seeds can be easily investigate such as pot , hydroponic and field conditions?
parameters , seed rate, seed density etc let me know the detailed and suitable methods
thanks in advance
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Drought resistance in maize can be evaluated through water stress imposition, measuring physiological traits, root phenotyping, and molecular markers associated with drought resistance. A combination of these approaches can help identify maize varieties that are more tolerant to water stress.
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How can maize contribute to food security, income generation and poverty alleviation in developing countries?
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Maize is a widely cultivated crop in many developing countries, and it can contribute significantly to food security, income generation, and poverty alleviation in several ways: -
1) Food security: Maize is a staple food in many developing countries, and it is an essential source of carbohydrates, protein, and fiber. It is an affordable and easily accessible food source for many people. Moreover, maize can be used to make various food products such as porridge, bread, and tortillas, making it a versatile crop that can provide dietary diversity.
2) Income generation: Maize farming can be a significant source of income for smallholder farmers in developing countries. Maize is a relatively low-cost crop to produce, and it has a ready market both locally and internationally. Additionally, farmers can sell maize in various forms such as whole grain, flour, and processed products, increasing their income opportunities.
3) Poverty alleviation: Maize farming can provide a pathway out of poverty for smallholder farmers. Increased maize production can lead to higher incomes, which can help farmers invest in their farms, pay for education and healthcare, and improve their standard of living. Moreover, maize farming can create employment opportunities in various stages of the value chain, from production to processing and distribution, which can contribute to poverty reduction in rural areas.
In summary, maize has the capacity to greatly impact food security, income generation, and poverty alleviation in developing nations. Therefore, it is crucial for policymakers and development practitioners to prioritize assistance to smallholder farmers in enhancing maize cultivation and market accessibility to fully realize the potential of this crop.
@Fayaz Hussain
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What are the genetic and molecular mechanisms of maize resistance to major diseases and pests?
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Here are a few examples of genetic and molecular mechanisms that contribute to maize resistance:
Plant-Microbe Interactions: Some maize varieties have developed resistance to fungal infections by forming symbiotic relationships with beneficial microbes, such as mycorrhizal fungi and rhizobacteria. These microbes can help protect the plant from pathogens by producing antimicrobial compounds or by inducing systemic resistance in the plant.
Plant Defense Signaling Pathways: Maize has several defense signaling pathways that are activated in response to pathogen or pest attack, including the jasmonic acid (JA) and salicylic acid (SA) pathways. These pathways can induce the expression of genes that encode defense-related proteins, such as pathogenesis-related (PR) proteins and enzymes that produce toxic compounds to deter pests.
Genetic Resistance: Maize breeding programs have developed maize varieties that are genetically resistant to specific pests and diseases. For example, some varieties of maize have been bred to contain the Bacillus thuringiensis (Bt) gene, which produces a toxin that is toxic to certain insect pests.
RNA Interference: RNA interference (RNAi) is a molecular mechanism that can be used to silence specific genes in pests, which can reduce their ability to feed on maize plants or transmit viral diseases. RNAi-based technologies are currently being developed to control several maize pests, such as the fall armyworm and the maize weevil.
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What are the environmental and social impacts of maize cultivation and consumption?
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Maize cultivation and consumption can have various environmental and social impacts, both positive and negative, such as:
Environmental impacts:
  • Maize cultivation can contribute to soil erosion, nutrient depletion, water pollution, greenhouse gas emissions and biodiversity loss due to intensive tillage, monocropping, excessive use of chemical fertilizers and pesticides, irrigation and land clearing. However, these impacts can be reduced by adopting sustainable practices such as conservation agriculture, intercropping, agroforestry, organic farming, integrated pest management and precision agriculture.
  • Maize consumption can also affect the environment by generating waste, emissions and demand for resources along the value chain, from production to processing to distribution to consumption. However, these impacts can be minimized by improving efficiency, reducing losses, recycling waste, using renewable energy and promoting a circular economy.
  • Maize can also have positive environmental impacts by providing ecosystem services such as carbon sequestration, soil improvement, water regulation, pollination and pest control. Moreover, maize can also be used as a source of bioenergy or bioproducts that can replace fossil fuels or synthetic materials and reduce greenhouse gas emissions and environmental pollution.
Social impacts:
  • Maize cultivation can provide income, employment and livelihood opportunities for millions of smallholder farmers and other actors along the value chain in developing countries. However, maize farmers may also face challenges such as low productivity, high production costs, market fluctuations, price volatility and lack of access to inputs, credit, extension and insurance.
  • Maize consumption can improve food security, nutrition and health for millions of people who depend on maize as a staple food or a source of animal feed in developing countries. However, maize consumption may also pose health risks such as aflatoxin contamination, micronutrient deficiencies or obesity due to excessive or unbalanced intake of maize or maize products.
  • Maize can also have positive social impacts by enhancing social capital, cultural diversity and gender equity among maize farmers and consumers. Maize can foster social cohesion, cooperation and empowerment through collective action, knowledge sharing and value addition. Maize can also preserve cultural heritage, identity and diversity through traditional varieties, practices and products. Maize can also promote gender equity by involving women in decision-making, income generation and leadership roles in maize production and consumption.
These were some of the environmental and social impacts of maize cultivation and consumption. However, these impacts may vary depending on the context, scale and perspective of the analysis. Therefore, there is a need for holistic and participatory approaches to assess and manage these impacts in a balanced and sustainable way
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How can maize be modified to enhance its nutritional and industrial value?
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Maize can be modified to enhance its nutritional and industrial value by various methods, such as:
  • Breeding: Breeding is the process of selecting and crossing maize varieties with desirable traits to produce improved varieties. Breeding can be done by conventional methods or by using biotechnology tools such as genetic engineering or marker-assisted selection. Breeding can enhance the nutritional value of maize by increasing its protein, oil, vitamin, mineral or bioactive compound content, or by reducing its anti-nutritional factors such as phytate or aflatoxin. Breeding can also enhance the industrial value of maize by improving its starch, oil, fibre or zein quality, quantity or functionality for various applications. Some examples of maize varieties developed by breeding are quality protein maize (QPM), high-oil maize (HOM), high-lysine maize (HLM), provitamin A-enriched maize (PVAEM) and waxy maize.
  • Processing: Processing is the process of transforming maize grains into various food or non-food products by physical, chemical or biological methods. Processing can enhance the nutritional value of maize by increasing its digestibility, bioavailability, palatability or shelf life, or by adding other nutrients or bioactive compounds. Processing can also enhance the industrial value of maize by modifying its starch, oil, fibre or zein properties such as viscosity, gelatinization, crystallinity, solubility, emulsification or the film-forming ability for various applications. Some examples of maize products obtained by processing are flour, grits, flakes, popcorn, tortillas, bread, porridge, snacks, beverages, ethanol, sweeteners, adhesives and bioplastics.
  • Fortification: Fortification is the process of adding micronutrients or bioactive compounds to maize grains or products to increase their nutritional value and prevent or treat deficiencies or diseases. Fortification can be done by adding synthetic or natural sources of nutrients or bioactive compounds during processing or after processing. Fortification can enhance the nutritional value of maize by increasing its vitamin, mineral or phytochemical content, or by providing functional benefits such as antioxidant, anti-inflammatory, anti-diabetic or anti-cancer effects. Some examples of maize products fortified with nutrients or bioactive compounds are iron-fortified maize flour, zinc-fortified maize flour, folic acid-fortified maize flour and anthocyanin-fortified popcorn.
These are some of the methods that can modify maize to enhance its nutritional and industrial value. However, these methods may also have some limitations or challenges such as cost-effectiveness, consumer acceptance, environmental impact and regulatory issues. Therefore, there is a need for more research and development to optimize these methods and ensure their safety and quality
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What are the best agronomic practices and cropping systems for maize production in different regions and environments?
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Maize production can vary widely depending on the region and environment where it is grown. Therefore, there is no single best agronomic practice or cropping system for maize production, but rather a range of options that can be adapted to the local conditions and objectives. Some of the factors that can influence the choice of agronomic practices and cropping systems for maize production are:
  • Climate: The temperature, rainfall, solar radiation and length of the growing season can affect the growth and yield of maize. Maize requires a warm and humid climate with adequate moisture and sunshine during its vegetative and reproductive stages. Maize can be grown in different climatic zones, from tropical to temperate regions, but it may require different varieties, planting dates, irrigation regimes and pest management strategies.
  • Soil: The physical, chemical and biological properties of the soil can affect the nutrient availability, water retention, root development and disease resistance of maize. Maize can grow on a wide range of soils, from sandy to clayey textures, but it prefers well-drained, fertile and slightly acidic soils with high organic matter content. Maize may require different soil management practices such as tillage, fertilization, liming and mulching depending on the soil type and condition.
  • Variety: The genetic characteristics of the maize variety can affect its adaptation, performance and quality under different environments. Maize varieties can differ in their maturity duration, plant height, ear size, grain colour, shape and quality, drought and heat tolerance, disease and pest resistance and nutritional value. Maize varieties should be selected based on climatic conditions, soil type, cropping system, market demand and farmer preference.
  • Cropping system: The arrangement of crops in time and space can affect the productivity, profitability and sustainability of maize production. Maize can be grown as a sole crop or in combination with other crops such as legumes, cereals, vegetables or forages in intercropping or rotation systems. Cropping systems can influence resource use efficiency, weed suppression, pest and disease control, soil fertility improvement and income diversification of maize production.
These are some of the factors that can determine the best agronomic practices and cropping systems for maize production in different regions and environments. However, there may be trade-offs between different objectives such as yield maximization, profit optimization, risk reduction and environmental conservation. Therefore, there is a need for integrated approaches that consider the biophysical, socio-economic and institutional aspects of maize production
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When you make crosses between two lines of parent maize and you have F1 generation,how to make effective selection ? on the panicle how can you that this grain is a product of crossing and this other grain on the panicle is not a product of crossing?
Or in the other sense , when you make drosses between two parents ,do all the grains on the panicle parent are served or all the grains on the panicle of female plant are product of crossing? How to make selection of individuals on a panicle?
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errata
effective individual selection
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I'd like to research about the reasons for high flour prices in Kenya and predict their future prices.
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Stochastic modeling is a useful tool for predicting future prices of commodities such as maize in Kenya. This modeling technique takes into account the various factors that can affect the price of maize, including weather patterns, market demand and supply, and government policies.
For example, weather patterns can significantly impact the production of maize. A severe drought can reduce the crop yield, leading to a scarcity of maize and higher prices. Similarly, if there is an increase in demand for maize due to population growth or changing dietary habits, the price of maize may rise as well.
Government policies, such as subsidies or import restrictions, can also affect the price of maize in Kenya. For instance, if the government offers subsidies to maize farmers, the cost of production may decrease, leading to lower prices for maize. On the other hand, if the government imposes import restrictions on maize, the domestic supply may be reduced, leading to higher prices.
Stochastic modeling takes all of these factors into account and generates multiple scenarios to estimate future maize prices. The model considers the likelihood of various outcomes based on historical data and current market conditions. By doing so, the model provides useful insights into potential future price trends and can help farmers, traders, and policymakers make informed decisions about production, trade, and regulation.
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In an experiment with 10 varieties of maize of 3 replications. The researcher taken the data of NCLB (Disease) incidence as score (0-9). In the scale, 0-9 are the percentage disease incidence on those treatments (varieties) viz.,
0- 0%
1- 1-3%
2- 3-6%
3- 6-12%
4-12-25%
5-25-50%
6-50-75%
7-75-87%
8-87-99%
9- 100%.
Taken this ordinal data! three times during crop cycle.
Now i want to know, among which treatments (varieties) there is statistical significant difference present in disease incidence and which treatments are statistically at par among eachother?
How can I calculate that?
Can I use Normal ANOVA for this non-quantitative data?
Thank you
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The Kruskal–Wallis test by ranks or Kruskal–Wallis H test (= one-way ANOVA on ranks) is a non-parametric method for testing whether samples originate from the same distribution (cited after: Kruskal–Wallis H Test using SPSS Statistics, Laerd Statistics).
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I am working with biochar. I have a plan to use biochar and microbes in acid soil in Malaysia for maize production. i have not much more experience to work with microbes. So i wish all experienced researcher openion regarding this issues.
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In acid soils, the microorganisms that present the best development conditions are bacteria, and by using biochar there is a great possibility of improving their development. In my opinion I recommend the use of bacteria that are associated with corn plants.
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Greetings, all researchers! Right now, I'm looking for technical assistance with my research. I've set a goal to assess agroclimatic indicators' current and future impacts on maize crop yields using the impact modeling approach. How can I correct agroclimatic indicator biases in raster data?
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There are several methods that can be used to bias-correct raster climate data, and the most effective method will depend on the specific dataset and the research question being addressed.
Some of the commonly used methods for bias-correction of raster climate data are:
  • Delta method: This method involves calculating the ratio of the model-simulated change in a variable to the observed change in that variable for each grid point. This ratio is then applied to the model-simulated data to correct for bias.
  • Bias correction factor (BCF) method: This method involves estimating the ratio of the bias-corrected simulation to the original simulation for each grid point. The bias-corrected data is obtained by multiplying the original data by the estimated BCF.
  • Quantile mapping (QM): This method maps the model output to the observational data distribution by matching the cumulative distribution functions of both datasets.
  • Bayesian hierarchical model (BHM): This method allow to incorporate information from multiple sources, such as observational data, reanalysis data, and model output to improve the estimation of the bias correction factor.
  • Machine learning (ML) method: This method includes a range of approaches including Neural Networks, Random Forest, etc. These methods have been proposed for bias correction and are being explored as a potential approach, but it is still ongoing research.
It's important to note that, regardless of the method used, bias correction should be done with caution and the performance of the bias corrected dataset should be evaluated in a robust way, through a validation process, and also to report the uncertainty associated with the bias correction.
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Impact of introduction of GMO maize in households
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I recommend according your project research to have an a good consultant in your country and to go more deeply in order the needs of your country.
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actually I need to measure the leaf area of Spinach, Maize and Tomato leaves, and leaf area meter is not available. therefore i need a simple and alternate methodology for determining leaf area. As i cited graph is used to measure. but i need more refine method if any. Guidance will be appreciated
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LAMINA (Leaf shApe deterMINAtion) is a tool to provide classical measures of leaf shape (blade dimensions) and size (area) as well as measures that indicate asymmetry in leaf shape and measures of herbivory damage (missing leaf area).
and
Black Spot is a free stand alone software and method to estimate leaf area from images of leaves captured using standard flatbed scanners. This easy to use software allows the user to batch process a large number of samples from multiple species with minimal user input.
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Formulas
Ca=(13.95*A665-6.88*A649)*1.5x10-3/0.18
(10-3 is raised power)
Spectrophotometer values at A665=0.451 & A649=0.436
Answer is -18.7
Cb=(24.96*A649-7.32*A665)*1.5x10-3/0.18
(10-3 is raised power)
Answer is-16.6
Is problem in spectrophotometer values or calculations?
Thanks
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Check your calculation again, perhaps the conversion (g to mg or vice versa). And also confirm that your formula is ideal
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The four accesions did not germinate across the three locations i planted. Please how do report this in my project discussion? and can i knock off the four accessions?
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It was supposed to check the percentage of germination before planting to make it easier for the researcher to make a decision.
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I am designing a trial for my PhD investigating the effects of vermicast on three commercially grown crops (onions, potatoes, maize). Soil transport will depend on the types of machinery used (power harrow, ripper, disks, potato harvester). I am unsure how long to make buffer zones between plots to avoid dilution of vermicast in the core sampling area. What search terms/phrases should I use to find research about the distance soil can be transported during passes?
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Thank you. I did a pilot trial last year and used 2 m buffer zones. I believe this was enough to keep the core sampling area free from dilution/contamination. However, I will be using plots for multiple years and each year will involve additional soil/vermicast transfer therefore I need to allow for this with larger buffer zones. The trouble I have is that I don't know how far soil is transported during cultivation, sowing and harvesting. Any research you can recommend on this would be appreciated.
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My Objective is to simulate the impact of climate change on maize yields in Isingiro District under future climate scenarios. 0.8435° S, and 30.8039° E
Where can I get the above R Scrit for simulation for RCP4.5 and RCP 8.5 from?
Will be greatful if any one who has can share with me
regards
Wycliffe Tumwesigye
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Hi Wycliffe Tumwesigye here is a script to download data from CMIP6 or CMIP5 using Python that can be useful for you:
"""
! pip install esgf-pyclient==0.3.0
!pip install requests-cache==0.4.1
from pyesgf.search import SearchConnection
import os
import requests_cache
requests_cache.core.CachedSession
#from pyesgf.search import SearchConnection
#import os
#import pandas as pd
import requests
from tqdm import tqdm
import xarray as xr
#import requests_cache
requests_cache.core.CachedSession
lista_modelos= ['ACCESS-CM2','ACCESS-ESM1-5','AWI-CM-1-1-MR','BCC-CSM2-MR',
'CAMS-CSM1-0','CAS-ESM2-0','CMCC-CM2-SR5','CMCC-ESM2','CanESM5',
'EC-Earth3','EC-Earth3-Veg','EC-Earth3-Veg-LR','FGOALS-f3-L',
'GFDL-ESM4','IITM-ESM','INM-CM4-8','INM-CM5-0','IPSL-CM6A-LR',
'KACE-1-0-G','MIROC6','MPI-ESM1-2-HR','MPI-ESM1-2-LR','MRI-ESM2-0',
'NESM3']
conn = SearchConnection('https://esgf-node.llnl.gov/esg-search', distrib=True)
ctx = conn.new_context(project='CMIP6',
experiment_id=['historical'],
realm=['atmos'],
variable='uas',
frequency='mon',
source_type=['AOGCM'],
variant_label='r1i1p1f1',
source_id= lista_modelos
)
ctx.hit_count
ctx.facet_counts['source_id'].keys()
# Obtain urls lists
lista_urls=[]
for i in range(len(ctx.search())):
try:
result = ctx.search()[i]
print(result.dataset_id,'..............ok')
lista_urls.append(result)
except:
pass
print('-------------------------------')
print('Lista finales')
lista_urls
# Download the first model for example
tr=[x for x in lista_urls if lista_modelos[0] in x.dataset_id]
files = tr[0].file_context().search()
lista=[]
for i in range(len(files)):
lista.append(files[i].opendap_url)
print(lista)
ds = xr.open_mfdataset([x for x in lista], chunks={'time': 120}, combine='nested', concat_dim='time')
ds_mei=ds.where((ds.lon>=-90+360) & (ds.lon<=-60+360) &(ds.lat>=6) & (ds.lat<=25),drop=True)
ds_mei=ds_mei.sel(bnds=1)
# Plot example
data = ds_mei.uas[0,:,:]
print(type(data))
data.plot.contourf(levels=35,cmap='jet',add_colorbar=True, x='lon',y='lat');
# Write the netcdf to a route
ds_mei.to_netcdf('uas_'+lista_modelos[0]+'_Historical.nc',format='NETCDF3_64BIT', mode='w')
"""
I hope it helps
Best regards!
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Hello specialist. I am working as a field technician for maize seed production. I would like to ask someone with experience in yield estimation of maize by tools or any calculator. I have been sawing on the internet, that people use satellite data, but I don't ins and outs of that. please introduce and guide me. Many thanks.
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Estimate planting population density, then multiple it x plant grain yield estimated. You can check on Google some papers calculating ear grain yield through ear dimensions. Regards.
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Dear colleagues,
I want to simulate the maize grain number with 6 level temperature stress experiments by APSIM-MAIZE. I have tried to modify the temperature fuciton from the default value (8, 34, 44℃) to a modified value (5, 30, 41℃), which concluded from Wang (2018).But, the simulation was not good for grain number.
Thus,
(1) how to modify the temperature function with my heat stress experiments?
(2) what's the calculation formula for maize grain number in the Maize Module?
in addition, my phytotron controlled-temperature experiment were conducted with 6 temperature from 30 to 40℃ during maize silking period.
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Hi, everyone my name is Tesfalem, MsC student at Warsaw university of technology. I wanted to do a research on maize grain storage which can be used to store for extended period of time without deterioration. Before selecting materials and do simulation of the storage I want you guys to tell me about how to model and simulate temperature distribution of the maize grain with in 1 m diameter circling.
Thank you.
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I am a agricultural adviser in Huambo in Angola, which is said to have the most fertile soil in Angola, what I can't see here really.
Wheat and maize were here planted for 2 years and irrigated with a pivot sprinkler system to ensure emergence in the dry season, but yields was yet with only moderate success. The corn yield was just as weak as that of other farmers in the area. I estimated that the maize yield is only around 1000 kg/ha. After the harvest residues, the wheat was only ¾ with a height of 1 m.
The soil with about pH 5, is a grey silty-sandy soil, presumably because the soil is 500 m from a river. But here isn't a laterite of rusty-red coloration like most soils here and that laterite are more remote from rivers.
My specific question is:
What is the best choice of soil preparation, fertilizing and which seed varieties to cultivate and gain best yields for wheat and corn on juvenile soil cleared before such acid and very sandy soils? Precipitation ist 1300 mm per Year.
The farm worker prefer to SEED and fertlize all by hand. Soil preparation and seed covering is done by harrow disks.
J HUMER, 18.7.22, Huambo
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For Example from one maize hectare of land we will get 80-120 qt/ha by using soil test based recommendation with recommended agronomic practice. But from one maize hectare of land we will get 25-35 qt/ha by using soil test based recommendation with recommended agronomic practice.
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Dear @Alemayehu Abdeta Keneni
The principal cereal grains contain about 10-14% moisture, 58-72% carbohydrate, 8-13% protein, 2-5% fat, and 2-11% indigestible fiber. Although these are typical values, composition vary depending on varieties of the particular grain, geographical and weather conditions, and other factors. Legumes and oilseeds are considerably higher in protein than cereal grains, and oilseeds also are much higher in fat. Various mature dry legumes and oilseeds contain about 20-40% protein; fat levels in peas and beans are low but are 20-50% in oilseeds.
During photosynthesis, plants produce glucose from CO2 and water in the presence of sunlight. The simple sugar then gets converted into complex carbohydrates; during the process the required energy is derived from oxidation of synthesized glucose.
For synthesis of protein and fatty acids and glycerol, the primary phosynthate is again utilized. Of course, in case of protein, nitrogen and sulphur are supplied from soil. However, the conversion requires much energy that come from breakdown of primary photosynthate (glucose).
For conversion of glucose into complex carbohydrates (major storage product in cereal grains), relatively less energy is required compared to those for conversion into protein and fats. Therefore, cereals are inherently high yielding crops than legumes and oilseeds. Of course, there exists other reasons that confer productivity advantage to cereals.
PS: This is my personal opinion, and you may disagree.
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screening for herbicide resistance and identify genomic regions associated with it in maize
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I am not expert in this field but I expect that even experts would need to know considerably more about your plans to be able to answer meaningfully.
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If you add a response, please do me a favor and answer the actual question. I've already searched the literature and haven't found anything. I already know what exudates are, and that they are different under stress conditions. To reiterate, I would like to know the SPECIFIC CULTIVARS of maize that have high exudation rates, under just basic field conditions, not any kind of stress.
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Root exudates comprise a large variety of compounds released by plants into the rhizosphere, including low-molecular-weight primary metabolites (particularly saccharides, amino acids and organic acids) and secondary metabolites (phenolics, flavonoids and terpenoids).
Exudates influence several factors within the soil such as nutrient availability, soil pH, and recruitment of bacteria and fungi.
Root exudates in maize are secreted depending on the kind of abiotic stresses such as soil acidity, salinity, drought, etc. Genotypic variations for root exudates are well documented in maize under acidity (Al toxicity), sodicity and drought. Specific Inbreds, populations or hybrids of maize with high root exudates under the given situation can be accessed through literature hunt.
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Gender in Agricultural development
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Gender issues are common in agriculture all over the world, mostly in developing nations.Women do most of the agricultural work and they are still paid less as compared to male for the same level of work. Particularly for maize,in my view knowledge level of both male and female in production may be the issue.
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Is there anybody suggest or describe this maize disease (cause) or Deficiency of minerals? This days common on some maize farmers in Ethiopia.
Thank you!
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Zinc deficiency photo University of Nebraska on maize.
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Or do you have knowledge or ideas where to get thiee MAIZE informations?
I see everywhere maize fields here with only very low maize yields, with about only 1 t/ha maize.
We in Austria have about 10 - 15 t / ha.
This Huambo region should be the best agricultural region, is written.
I am advising a new farmer here, but there is no knowledge about this requirement.
Many Thanks
J HUMER
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I want to determination the root activity of maize, who knows a fast way to determination the root activity? Thank you for your kind help and comments?
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Introduce a hydroponic and grow maize. it will be easy for observation
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The advantages of using maize for Biogas
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Maize in the form of silage provides high yields (10–30 t of total solids—TS per hectare [1–3]) and is thus a suitable energetic crop for biogas production.
Regards,
Shafagat
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Struggling to get good quality DNA from dried maize leaf samples. Used modified CTAB protocols, without liquid Nitrogen but had smears on my gel. Anyone to help with a protocol that works best for extraction of DNA from Maize leaf samples?
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Aditya Sarkar Thank you. Just noticed that the protocol uses both CTAB and SDS detergents. Will give it a try and provide feedback
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I a Wifi Thermometer which is portal with an API for data sharing. I would to use it in an experiment for Maize grain storage facility (a shade).
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Luis Daniel Lepe thank you so much very helpful site
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Dear Experts
At the beginning of fermentation, the pH of the mixture of barley and maize is 4.5. However, the pH of barely alone is 5.3, and maize is 5. 6. Thus, what are the possible reductions in pH in the mixture?
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Dear @Reda Nemo You should access replies to a similar question at:
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I am based in North West Province, South Africa. North West is a land locked province, with mainly summer rainfall and dry winters.
Summer is around September to March and Winter June/July to August.
This year we had more early winter rains. This is not normally the case.
I have started seeing these caterpillar/worms with a black body, with hairs al over, with some white hairs on the body.
What are these caterpillar/worms? Are they harmful to maize, soya, sorghum or sunflower seed/plants?
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Thank you for your answer.
Do you possibly know the family, genus and species? Also, if you have an image or diagram of the lifecycle and the amount of time between stages.
I would also like yo know their natural habitat, or preference, as well as how to spot their damage on either leaves, stems or grain. As in specific markings they leave behind when feeding or the appearance of their excrement/residue.
This would be very helpful
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Dear colleagues, I'm looking for published statistics on global percent area used for production of wheat, rice and maize. I know that there are some data on global production and data on estimated global irrigation that I could use for an analysis. However, I would like to see first if there is this information already published by someone.
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For an assay on young plant
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Dear Vanessa,
You must prepare a suitable spore suspension of the pathogen Fusarium verticillioides, put it in a suitable volume sterile syringe and then inject the spore suspension in the plant stem near the tip. You need some time to see the symptoms appearance.
Wishing this method is useful.
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Dear friends,
I am doing a PhD and one of my objectives is to use DSSAT for modeling effect of Climate change scenarios on maize productivity in SW Uganda. My study area lies btn
0.8435° S, 30.8039° E
Where can I download the above current climate scenarios datasets for simulation of impact of cc on maize yield ( early, mid and end of) century?
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Also, you can download CMIP6 ensemble data and/or its members from Copernicus (https://cds.climate.copernicus.eu/cdsapp#!/dataset/projections-cmip6?tab=overview).
Good luck on your PhD.
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Dear colleagues,
I would like to ask if there is a Standard, guide, manual or well-explained procedure to do the "pot experiment", also known as "plant growth experiment" (there is another name for this experiment?). Additionally, if there is a special one for maize growth, that would be great.
In fact, I read several articles where this experiment was used, however, in none there was mention of a standard, protocol or manual. I am kind of surprised about it, as this procedure seems quite important.
If you have any tip, article, thesis or any trustable document that I could use to guide me, that would be great. I plan to do this experiment with a collaboration, however, I need to know better the procedure to plan ahead.
I thank you all in advance for your time and attention.
Have a great week.
Kind regards
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Luanna, I expect that there's a lot of literature on potted maize trials, and I suggest you compare their experimental designs with the facilities used and the variability that was encountered. There are some principles to apply to your own growing conditions, rooting media etc. (which I believe in having done many greenhouse trials with potted eucalypts in Portugal - I have no experience with maize). Try to do pilot studies to understand your own total variance, and to get an idea of how to partition it in your experimental designs. Personally I'd do several smaller trials rather than fewer bigger ones, so that you can observe the variation between trials, and similarly I'd keep the designs relatively simple - eg. randomised complete blocks. Finally a couple of very basic points - make sure you have plenty of error degrees of freedom, and if there is any mortality make sure that the number of plants per plot is sufficient to avoid missing values or inflating the error variance.
Philip
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  • We expressed a maize CC-NBS-LRR protein in Nicotiana benthamiana by injecting the Agrobacterium strain GV3101 with a maize CNL protein into the leaves, cell death was observed after 3 days without the presence of the cognate pathogen effector, Does this happen often?
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Thanks a lot!
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In general fall army worm will feed and cause damage by feeding in whorl of Maize. So we are recommending whorl spraying of different mode of action insecticides against FAW. But recent some studies found that whorl application of insecticides by mixing with sand is best option. Is this really effective??
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Better option is to identify FAW tolerant genotypes although it is very difficult. Among the tested 40 genotypes, one hybrid was less infested by FAW. We are trying to promote this one and already proposed to release for commercial cultivation in Nepal.
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Hi everyone! I wonder if anyone could indicate me an efficient and simple protocol for separation of leaf tissues (epidermis, mesophyll, vascular tissue) that could be used for crops, such as tomato, soybean, maize, etc.?
I appreciate any suggestion given.
Kind regards,
Aline
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Rapid and simple isolation of vascular, epidermal and mesophyll cells from plant leaf tissue
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What are the implications of using maize-cobs for mulching? Does it rob the soils of its nitrogen?
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Yes, I fully agree with Paul, to remove excess N you may very well use as mulching.
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I intent to subject maize seeds to mutagenic treatment and need advice on how to characterize the mutant lines using genetic tools
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For how long maximum nitrogen is required by crops like wheat and maize. Then how long urea maintain N supply in soil?
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Perhaps , grain filling stage is most crucial with respect to nitrogen use ,in addition to tillering stage , depending upon other agro-pedologcial fcators. It would be better , if talk in terms of agronomic efficiency, physiological efficiency , apparent recovery , utilization efficiency , upatek efficiency ...like that, so many terms , depending upon their field of specialization , researchers use
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Root length, root biomass and types of root determine the nutrients absorption, tolerance to drought and lodging and ultimately optimum plant growth and productivity. Is there any simple field based procedure to grow maize plant in field and to determine total length and biomass of root? An efficient and simple protocol may help in screening maize germplasm for root parameters.
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Dear Dr Narendra Kumar Singh,
Greetings, this stage is the most important one as its is the last stage in plant life. I have done the same stress in wheat after anthesis.
For estimating the root traits their is some software that could help in getting the data. So, try find it. Their is some free software .
with best regards
Khaled
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Last year during winter planting in some of the areas in Terai region (plain southern part of Nepal) we faced tassel ear problem in maize.
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Deae Dr. Singh,
There was no frost but temperature was below 10 degree C at V5 to V6 stage.
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Dear RG scholars, GREETINGS!!!
  • I'm relatively new to CROP MODELING and Simulation
  • As you all know, modeling is so interesting but as wide as an ocean.
  • Modeling includes many challenging topics such as model calibration, parameter estimation, reliability assessment, data collection design, and a lot more.
I'm doing my research on "Model simulation of maize growth and yield using DSSAT-CERES-maize model v.4.7.".
No model was done before for my location.
I'd two-year experiments using 3 maize varieties and 5 nitrogen rates for a new
(same location but different years).
  • 1st year for model calibration
  • 2nd year for model validation
N.B.
  • My maize is rain-fed maize
  • I've 30 years of climate data (RF, Tmax & Tmin)
  • I've soil and plant lab analysis results
What parameters, mandatory data, and what max and min values should I have?
What vital steps should I never forget?
What yield components of maize should I focus on?
Your experience, please? And any relevant reference you think is good for me!!!
STAY BLESSED!!!
Alem
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High Alem,
Interesting to start discussions!
I recommend you first to read the manual. What type of soil sample ( profile or surface)? It is the profile data you need. Crop data is highly important and there are variety specific parameters. You have to know that! I knew for the varieties BH540 and BH660 for example in Ethiopia. I hope you know Dr Kinde from CIMMYT Ethiopia, he will help you a lot about DSAT as he is expert of it.
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In India the soil health cards are being issued to farmers since last 6 years. However, it is not possible to have soil health cards for each and every field till today. We know that whenever we have a bumper crop of sorghum, maize, rice, wheat etc. the soil nutrient status changes after every crop season. Under such situation it is required to measure the soil nutrient status after every crop season. Therefore, we need to have a fast and accurate measurement system which can provide the soil health card to the farmers in his field itself so that he can plan for fertilizer application rate just before the crop sowing season. Under present laboratory analysis method it takes months to get the soil health card and by that time the crop sowing season is over.
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See also the following very good RG link:
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Nitrogen is the most limiting and the least efficiently used (NUE is 0.3-0.5 in developed countries) plant nutrient.
But what most gets me in doubt is the time of nitrogen fertilizer application.
  • Some say before planting (African Farm Resources Center, attached herewith)
  • Most say during planting (N.B. maize takes 6-9 days to emerge, but still not active to use fertilizer)
  • Only a few say after planting.
Which one is scientifically correct? Please include references!!!!!!!
==============================================================
N.B.
I don't know if Nitgen fertilizer has other uses in the soil than as food for the plant. Some well-experienced farmers have told me that Nitrogen dehydrated water-logged soils; "It ignites the heat that helps seeds to emerge profoundly"!!!
============================================================
Appreciate your valuable help for all in doubt like me!!
Alem
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Dear Dr. Redda,
Very nice and practical question you raised. Normally, agriculturists recommend 50. 100 and 100% of N, P205 and K20, respectively as a basal dose. As you mentioned, until and unless plants have roots they can not utilize the applied fertilizers. Potassium and phosphatic fertilizers takes a little longer time to to be ready to the plants. Therefore they must be applied as basal. There should be wise application of Nitrogenous fertilizers as they are lost through volatilization, leaching and run off water. If Phosphatic fertilizer is complex one with some amount of N, it is not necessary to apply additional N through Urea or other sources. This is the reason, instead of applying 50% N as basal; increase the number of top-dresses at various stages. It will automatically increases the N use efficiency by crop.
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Given the diverse use of maize for human consumption, animal feed and industrial raw material, is maize is going to play as a major rabi crop in the coastal region in general and coastal saline region in particular.
Please give comments on how to increse the maize acerage and reduce the fallow area as well as increase farmers income.
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Maize can be grown everywhere if weather condition (extremely low and high temperatures) is not a limiting factor.
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  • Five cereal crops provide 60% of the daily food intake worldwide!!!
  • Three cereals (rice, maize, and wheat) cover more than 50% of food intake.
  • Maize is regarded as a "Hunger-breaking crop", particularly by the poor farmers of Africa.
  • Today, climate change is the biggest threat challenging agriculture. So, which crop is more vulnerable to climate change (rainfall, temperature, etc.)?
  • Maize is getting wider destinations even to new places due to its special characteristics. What do you think are its special merits for its popularity?
  • Which of the 3 popular world cereal crops would be the 1st in 2050 and/or 2100? Why?
Thank you for all your valuable responses!!!
Alem
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Considering the global context of climate change, maize crop can tolerate various stresses such as heat (higher temperatures), drought, water and others. This crop can save life of both humans and animals as it can be used as food, feed, fodder and fuel. Therefore, it can be predicted that maize will be the number one crop in the future.
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In my case, we use 150 kg ha-1 for OPV and 200 kg ha-1 for hybrid varieties are recommended to be applied with three splits: 1st at planting, 2nd at knee height, and 3rd at flowering (silking and tasseling). But, still, farmers of the lowlands (hotter areas) use less than this rate and generally miss the 3rd split due mainly to fear of the burning effect caused by high temperature and/or low moisture.
What is African Farm Resource Centre telling us?
"For hybrid maize, 400 kg ha-1 of NPK is recommended to be applied just before planting, which should be followed up four weeks after planting with 250 kg ha-1 of CAN as side-dressing."
400 kg ha-1 before planting + 250 kg ha-1 after planting"
  • How is this possible?
  • Is that logical? I don’t understand it!!
  • What about the law of diminishing returns?
  • Could poor farmers afford this? Is this acceptable by farmers?
  • What would be the benefit-cost ratio of this? Is this economically feasible?
  • Nitrogen fertilization is the main cause of Carbon Footprints in agriculture. Did African Farm Resource Centre consider this?
Many thanks and respect to all RG members!!!
Alem
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In case of Nepal, 180:60:40 NPK kg/ha has been recommended for commercial hybrid maize cultivation. Urea, DAP and K20 are used as sources, respectively. The 18% N in DAP works in initial stage. The whole Phosphorus and potash should be applied during last field preparation. It will be better to apply N fertilizer in four splits: I: First weeding II: Knee high stage III. Prior to silking and tasseling and, IV: Initial grain filling stage. Each time, top-dress from opposite side of the row where previous top-dress was carried out. Cover the N fertilizer immediately after top-dressing. Irrigate the field before top-dressing if needed, and not just after top-dressing to protect nutrient from leaching.
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What is the optimal temperature for grain filling stage in maize? How lower temperature than optimal at this stage affects plants?
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When the issue of intercropping is raised, most researchers, including myself, are addicted to the idea of intercropping cereals with pulse crops and the first reason that comes to our mind is the "nitrogen fixation", i.e., restoring the fertility of the soil.
But when doing my research on intercropping, I had the chance to come through seeing farmers intercropping maize with sorghum (obviously, both are cereals). I was really surprised a lot!!!
  • What do you think is the farmers' reason?
  • What other forms of intercropping did you know? What with what?
  • What advantages (reasons) of intercropping do you know beyond the well-known fact of enhancing soil fertility?
Wish you all the best this life can deliver!!!!
Alem
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Dear Alem,
Intercropping in general is a delicate topic I think. To my opinion, it shall be applied only if the combined crops can benefit from synergies or side effects from the other (not mixing randomly any crops without reasoning). For sorghum and maize, I agree that it might not be the best option, and a maybe a more obvious combination would be to combine cereals with leguminoses... However, maize and sorghum are needed in higher quantity by many growers serving as animal feed (plant residues) and carbohydrate source (grains), especially subsistence growers. I think there are several reasons to combine both. Literature indicates there can be better weed control, better water use efficiency, and even higher yield or higher silage quality. Of course, when managed inappropriate or having bad luck with the weather, there can also be negative effects. Additionally I would also add decreased disease spread compared to monocultures. If you have time, you might review some literature, to confirm my hypotheses ;)
Cheers, Elisa
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I'm doing my research on modelling of barriers (limitations) and determinants (social constraints) of maize farmers' adoption of adaptation options to curb the negative impacts of CVC (climate variability and change) using the MNL model.
For the results of the MNL regression model, I'm using the assumptions of IIA (the Hausman test), the VIF, and the contingency coefficient that need deep result interpretation.
But in the STATA software, both the terms contingency coefficient and correlation coefficient appear, of course with different values.
What is the conceptual difference between the contingency coefficient and correlation coefficient? What best example do we have to differentiate them for better understanding?
THANK YOU SO MUCH!!!
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Alem Redda that’s why they are called independent
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We know rice is a C3 plant. In context of rising global temperature, C4 plants (maize, sugarcane) are likely to have some physiological advantage over c4 plants. Rice scientist have been working to develop c4 rice varieties. What is the prospect of c4 rice varieties in rice growing Asian countries?
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Nice question. This is IRRI s most ambitious research program...and if we succeed , it will be breaking the plateau in rice productivity with close to carbon neutral ability of rice .
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What number of days to 50% silking can be used to predict the maturity of maize? for example early maturing verities may have a range 56-63 days to 50% silking.
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In our case, just you double the number of days to 50% silking that will be the tentative days to physiological maturity. For example, if days to 50% silking of a variety is 60, then the days to physiological maturity will be 120 days.
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I performed a fungal and enzymatic treatment in the lab and now I will do this pretreatment in the field. In my opinion if we add the fungi directly to the field there will be a great risk of contamination. Or, even if there will be contamination, the control will give us a difference?
thanks in advance for your feedback
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Then I recommend you conduct the research in screen house.
The name of the fungi is very important, is the fungus in question serious pathogen in your country? Is it of quarantine important? If no, then you can try it on the field. Higher temperature may also influence the results.
Review some partinent literatures and use the appropriate methods. I wish all the very best.
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I'm looking for a study on the utilization of cover crops or green manure in corn fields that was conducted in China. I'm curious to see how cover crops or green manure affect the SOC over there. I'd love it if someone could send me some papers or other information. My main interest are cover crop/ green manure, SOC and Corn/maize.
Thanks
Best
Deepak
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Meta-analysis of green manure effects on soil properties and crop yield in northern China
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We are having a seed dryer soon in our Farm. In our assessible markets, grain dryers are common but getting choices for seed dryer is not. I am concerned about germination of seeds if dried in grain dryer which doesn't have temperature control options. Please suggest me mechanical seed drying options suitable for a farm that produces seeds in about 30Ha of land.
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La temperatura que se sugiere es 15 grados celcius
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I've tried proline determination assay (Bates 1973) on maize leaves, but it didnt work. Though it worked on maize pulverized seed (not lyophilized). Anybody knows if there's something wrong with lyophilized tissue and this specific method? or something with lyophilization procedure?
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Have you tried doing a proline analysis on fresh tissue and with added proline (increasing amounts might be instructive)? Make the same samples then lyophilise them and analyse for proline. that should show if it is lyophilisation which is the problem. P.S. do good replication and perhaps different methods of lyophilisation.
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When you looked at the CV of the yield component data including height is very low compared to the grain yield, then it is very difficult to generate data that relate final yield data with height for example. This is due to subjectivity of the researchers! Is it not better to fix at office where to take the plant sample ( plant height ) ?
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I have experience with big-size data of rice. I took yield and yield components including plant height data both at the field (at the dry condition) and office lab. So you can take a random or prefix sampling for yield component data of big cereals like maize, but precision will be reduced due to fixing or reducing sample size.