Questions related to Lizards
I got some lizard specimen which had been stored in 95% alcohol for over 15 years at room temperature. Also, the lizards were all being preserved in the same container, while their legs were removed for karyotype experiments, which means there are some holes on their body. My question is that could these samples be applied in Sanger sequencing? Or even NGS? I wonder if this bad storage condition affect the DNA among these samples. Thanks!
This is mentioned quite a bit on the internet, mainly related to captive care, but I’m having trouble finding anything published.
Thanks in advance!
Is promoting the rearing of small-sized animals’ (micro-livestock) a viable option for addressing the issue of climate change adversity in smallholder farming sector in Africa.
In this context micro-livestock refers to small indigenous vertebrates (goats, sheep, rabbits, guinea pigs, poultry (chickens, ducks, guinea fowls), etc.) and invertebrates (snails, rodents, lizards, insects, etc.) both domesticated and wild genetic animal resources which may be produced on a sustainable basis for food.
I already have 16s sequenced for the particular species I am working on. The literature I have found has used beta-actin or 18s as a housekeeping gene in RTqPCR. I am wondering if I can use 16s instead? Will make my life a bit easier.
I have seen that in Gekko gecko they use 1 volt to obtain seminal samples, but I search for Phyllodactylidae individuals that weigh 6-7 grams and measure 5-7 cm.
I was wondering if anesthesia (most likely isofluran) of animals before euthanasia and sampling of internal organs (here reproductive tracts in lizards) for RNA seq might ater the mRNA expression profile?
Would you recommand to perfor the euthanasia without the anesthesia or would you anesthetized them?
Thanks a lot for your answers,
I'm looking for protocol to extract DNA from liver tissue sample of geckos that have collected and preserved in 95% Ethanol. I also want the protocol along from DNA extract until sequencing of nuclear DNA and mitochondrial DNA. Please help
I recently began to work with viviparous Neotropical skinks of the genus Mabuya. Specifically with females at different stages of gestation. My tutor and lab colleagues have studied them for a long time and a recurring comment is related to the difficulty of field sampling.
The standard method of catch is by hand, but these lizards are very quick moving through the litter, and their smooth-scales covered skin makes them difficult to hold. Also, in the most advanced stages of pregnancy, these lizards stops feeding so funnel traps will likely be less effective.
I have little experience in catching, and I am planning some field trips to obtain some specimens (especially to learn about the field work). I would like to try different catch methods, hoping to make it easier to obtain research material.
I will be very grateful for any suggestions or advice you can provide.
I know there are different structures referred to as "eyelids" in animals, specifically reptiles. However, I had been under the impression that the eyelids found in Eublepharidae (Gekkota) were more plesiomorphic than the fused spectacles found in other gecko genera, including but not limited to Rhacodactylus and Phelsuma. Is this accurate? I can't find any research expressly stating so (or the opposite), at least not without paywalls.
Thank you in advance!
South India s monitor lizards are not studied closely.If any one is interested or doing work on sand goann's,I am interested to call them.
How ecologist and herpetologist can observing small specis. For example, these last week we have descovred a new chameleon species in Madagascar.
This new reptile is become the smallest amniots in the world - the specis : Brookesia nana !!!
Can you give us your opinion about this question?
I just recently started working with the different types of home range estimations and have a few questions about this and that. Maybe you could help me out with that?!
My study animals are Sand Lizards (Lacerta agilis), that I tracked with VHF transmitters along a railway track in eastern Germany. I tracked up to 20 animals at the same time, that’s why I have only 3-7 datapoints per animal per day. The transmitters lasted up to 20 days, but most of them were peeled of by the animals earlier. In average I have 33 datapoints per animal. With this information in mind, you can hopefully get an impression of the data quality I am working with. So here are my questions:
1) I read that it is important to report on autocorrelation of the datasets (and also on site fidelity of the animals). The dataset of most of my animals seems to be autocorrelated. This is probably due to the site fidelity of the animals. My question is: How do I interpret this information about autocorrelation and how does that affect my home range estimation?
2) I would like to check if the number of locations was somehow sufficient to calculate proper home range estimates. Therefor I would like to use “area-observation plots”. I am just wondering what to have on the y-axis: if I have this plot for an MCP analysis (for example), do I take the total area of an animals MCP (in m2) or do I use percentages (where my final MCP is 100%)? In the second case, an asymptote would probably have more that 100 % - is that correct? Additionally: Is there a way how to calculate the number of locations randomly from my dataset (for every single animal) or is that usually done just one by one in the same order as my sampling occurred?
3) During the sampling I took notes when I sighted the animal I was tracking. Is there a way to include this information in any home range estimation? Do you think that is a useful information at all?
I would be really glad if you have at least one or another comment on my questions or could recommend some literature on these topics. Thank you very much!!
I am working on peptides secreted from frog skin. The peptide under study is secreted from the skin of Australian frog - Uperoleia mjobergii. I would be very grateful if anyone of you could let me know what is the pH of frog skin. Thank you very much for your help.
We are analyzing the response of >400 respondents to a survey, with values of -1 (no) 0 (neutral), and +1 (yes). The data were collected to assess opinions on feeding practices at zoos. An example of what we want to get answers to, could be whether a respondent would prefer to see live insect feeding to a lizard on exhibit, or would prefer to have this done off exhibit. Thus for this question, and others, the data in excel is sorted as illustrated in <Capture neg pos ranks>.
Our first analysis would be to compare differences between these variables; this would be followed by comparing each variable to others (gender, age, nationality, and so on). Any help/suggestions on the choice of test we want to use, i.e. we were thinking of the Chi square test, would be greatly appreciated. Stay safe!
I am currently working on a research entitled: “Taxonomical study of some true Lizard family (Lacertidae) species in the Syrian coastal region, using peripheral blood cells morphology”. And I am facing many difficulties such as the lack of classification keys for this family or a field guide to differentiate between its species,I could not obtain an approved classification key, Therefore, I am writing to ask if you can provide me with a classification key to help me completing this research.
I placed the word "eggs" in quotation marks, because maybe they are not eggs... These structures shown in the photos were exposed in a sand dune by the wind in the Negev Desert, Southern Israel. They look calcareous with sand attached to them and they are quite hard and elongated. They are thicker than normal hard-shelled reptile eggs (e.g., geckos, turtles etc). They don't look like soft-shelled reptile eggs, that tear and look like an empty paper bag when they dry out (like Varanus eggs). But the most disturbing character is that they are not round in a cross section, as are all reptile (and bird) eggs that I have seen so far. All of them (found on three different occasions) where flattened in the same way and not round in a cross section.
I will be glad to hear from anyone who has seen something similar elsewhere or has an idea for a process that could lead to form these structures (maybe accumulation of calcium on something else, not necessarily an egg?).
I am working on a grant proposal for undergrad. My idea is to try to identify the process by which crested geckos (C. ciliatus) undergo parthenogenesis, and why it often fails. I have read that parthenogenesis in reptiles sometimes occurs as a result of hybridization.
Hi I'm trying to heat a chamber (about 100 Liter volume) with ceramic IR lamps (6 units of 125w each). The goal is to have an approximately homogenate heating at the bottom of the chamber (50cm from the lamps at the moment). But is not so simple, although I manage to get the intended temperatures, on average, the central area of the chamber can surpass the borders by 4 ºC, which is a bit too much to call it homogeneous space. Which is understandable, because even though the lamps are evenly distributed through the top surface, the commonly heat area on the centred ends up receiving more heat. The question is, how to receive the heat from these 6 spots (the six lamps) and distribute equally? I've been thinking of placing a metallic mesh (2mm pores) between the lamps and the chamber, to try to have a layer of heating on top instead of the 6 spots of heat... would that work? I have to be careful with this layer, because too much isolation would result in massive overheating of the lamp side to get the intended temperatures on the chamber side. Has anyone faced this kind of unequal heating? how did you solve it?
All opinions are welcome.
Cheers, Luís Pereira.
I can't find the original research where Rhacodactylus ciliatus was placed in the genus Correlophus. But I know it happened recently.
I am working on a project involving parthenogenesis in crested geckos (Correlophus ciliatus), and I plan on comparing genes- particularly STRs- in samples from the eggs, the potential parents, and previous records (via GenBank, etc), to each other. However, I do not know how to successfully isolate DNA from a very early embryo in a shelled reptile egg. I will likely have to do this, because all the previous parthenogenic eggs from my geckos have failed long before complete development. Please excuse my phrasing, this is not a subject I have much experience in yet.
Deseo comprobar si las excretas de los geckos
Hemidactylus frenatus en las casa de un municipio de Honduras son portadores de Salmonella spp.
I am desperately searching for results about the foraging mode (active foraging, ambush or mixed) of Sphaerodactylus lizards, geckos from the America for which data seem poor in the standard literature. I have already searched books, reviews and the google scholar but maybe someone here is aware of a good but difficult to find reference
The brain tissue its from a lizard. We want to see de serotonin mark in the pineal complex.
What are the most effective methods for permanently marking small lizards for population monitoring (in my case skinks approximately 60 mm SVL). Are there reliable alternatives to implants or toe clipping?
Hi, can anyone please help us identify this species of mites found on Moroccan Geckos in the Anti-Atlas mountains. We're actually seeking collaboration with experts to finalize some works on prevalence of these ectoparasites in different Geckos populations.
I'm working on counting the number of neurons and volume of the optic lobe in lizard embryos. Currently I have sectioned one embryo's head for the pilot study, but I don't know what to do from now on.
I'm going to use the physical disector without a specialized software.
I was instructed by a researcher that has been helping me to section the tissue for the pilot with 30 pairs of sections. The paraffin embedding protocol shrink the tissue more than I thought and I only got 17 pairs of sections.
Now, I don't know how to sample the pairs of sections, as I don't have a motorized stage available. Is there a technique for manual sampling for the physical disector? And also, how to define the distance between the probes in the pilot? It is estimated using 100x for what I've been told, but I noticed that although the layers are quite distinct, the nucleus of the cells are not clear and the tissue is a bit blurred at 100x. Is this a problem to count the cells?
I read a paper by Brown (2017) that helped understand how to estimate volume with 4x objective lens. Is it still possible to use the same sample for both probes (Physical disector and Cavalieri) in different objective lens?
I hope someone can help me. Thanks!
How does one distinguish between cursorial and generalized terrestrial modes of life in lizards? I know that in mammals it is relatively easy to distinguish between a cursorial and non-cursorial species based on the former's much longer limbs, but this is less obvious in lizards. So how would one distinguish between a cursorial lizard like a collared lizard and a non-cursorial species like a chuckwalla or a monitor?
I performed a mark-recapture survey at four sites (of two different sizes) for one species of lizard, and now have data for - total survey time, survey area and number of individuals encountered. Is there any way to calculate survey effort from this data?
I intend to revisit the sites and assess if there has been population decline; so quantifying the search effort each year is key to support any findings.
Hi everyone. I have two quantitive variables: population density (of a gecko species) and refuges density. The first one has values from 0.0012 to 0.02, and the other one, from 0.33 to 17.66.
In order to obtain a better scale to observe my data in the scatterplot, I transformed these variables to log. Actually, they looked better after the transformation. But, when I compared the R2 of these two linear regressions (one with log transformation and the other one without it), the R2 of the untransformed data was higher (R2= 0.32) than the R2 of the transformed data (R2=0.20).
So, in this case, which one I should use? Regression with transformed or untransformed data?
Thank you for your time!
We have a verified record of more than 40 years in a captive Uromastyx (exact species identification still in process). We would like to know how this record compares to the longest known life times in this genus. Thanks to help us find published records (with exact literature reference) or unpublished data!
I am doing a research project for my grad studies and I plan on feeding the specimens four different types of food in seperate terrariums.
I wish to purchase a smartphone-compatible microscope, primarily for counting scales on small lizards, identifying invertebrates etc. A low magnification capability (e.g. 5 or 10x) is more important than high. A lot of the devices I've seen online start at (e.g.) 50x zoom, which is generally too much magnification for my needs. My ideal would be something that can be attached to a stand, has built-in LED, and if possible also doubles as a borescope (to investigate tree hollows, burrows etc.). I plan to use it for field work, hence the desire for a multi-purpose device. Please can anyone suggest something suitable?
I'm attempting to use a Biomeme two3 portable qPCR machine for SNP genotyping as part of an MSc project. I am using DNA from lizard blood samples purified with the Biomeme M1 purification kit and PrimeTime primers and probes designed and manufactured by IDT for our SNP of interest.
The amplification curves we've obtained so far look extremely strange. Our most recent runs consistently show a small, early increase in fluorescence around cycle 10, which almost immediately levels off (after 2-3 cycles). Two previous qPCR runs (with the same reagents) show patterns of increasing and decreasing fluorescence which seem almost random - at the time we assumed this represented background noise, but the relative changes in fluorescence were actually much higher than for more recent runs, suggesting that perhaps something more was going on?
Any help interpreting this somewhat perplexing data would be much appreciated!
My e:mail is email@example.com or home e:mail firstname.lastname@example.org I retired about 5 years ago and am emeritus at USGS and to to the office about twice a week now. I am trying to finish up all the loose ends of projects I started some years ago and work on a book of the herps of Nevada.
My questions pertain to repeating genetic sampling of the Rock Valley lizards and rodents now.. Do you have any thoughts if it would be worth the effort? I did gather samples of Whiptail tails and rodent ear clips some years ago.
In particular, I am having difficulty finding data on vitamin D and K.
- wild avian
- wild rodents
I am searching for ways to promote lizard and amphibian capacities as a profit from the restauration of their habitat in private gardens.
Therefore I need evidence of these actions
I am interested in learning standard procedure for stomach content analysis.
I have work on sonotaxonmy and biomechanical based on ambhibian .Some tillte knowledge any other lizard but some query after research .
It is common in species catalogues to cite the first page of the work where a particular species was originally described. Although in many articles we see the first reference to the new name in the abstract or in different parts of the main text, we usually cite as the "first page" that where the "Description" is set, citing the holotype, type locality, etc. But what about a new species whose description is placed in an unpaginated supplementary material? This question arose when reading the recently published article describing a new lizard species, Ameivula apipensis: http://onlinelibrary.wiley.com/doi/10.1111/zsc.12277/full
I am planing to look at reproductive cycle in females lizards (Podarcis muralis). My goal is to asses clutch size, ovulation and differentiate between late and earlier stage of pregnancy.
I checked this couple of options of ultrasounds machines:
Sonsonite Titan Portabl
However I heard that they are not as precise as thought. I was wondering if someone has any experience with this machines or others of this type ( portable, since I will be in the field) and can give me some advise
Thank you very much in advance ,
I am planning for a morphological study on Saara hardwickii in the Aravallis in Western India. It has a semi-arid landscape with a fairly good population of the species. But capturing these species became a problem. I need to know some methods by which I can capture these species for morphological study and then release safely in their habitat.
I have to calculate the distance an animal moves in a box over a 4 hr time period.I need to pull the data off a 4 hr long webcam video of the animal moving between two chambers in a box (area fixed). There is a divider in the middle of the box, so you cant see the animal for a few seconds as it moves to the opposite chamber. The movement isn't linear.
Can species of Lacerta viridis complex hybridize at all? And if yes, then can Lacerta viridis hybridize with Lacerta bilineata, Lacerta strigata, Lacerta trilineata?
Can you recommend literature on this topic?
Are there any morphological features to use to distinguish Podarcic taurica from Podarcic muralis in the wild nature and to distinguish species of the Podarcis genera without making genetical and molecular analyses?
I have an undergraduate student who is sorting through some fossil lizard jaw bones, but identification to species (or even genus) is challenging. Does anyone know of a good resource (publication or person) that they could suggest that might help in this regard? Thanks.
A few years ago I have seen the above mentioned plot (Snout Vent vs. Julian Date) in a Herpetology book, but I can't remember now which book it was, nor how was the plot called. The plot may be used to determine cohorts and to see patterns in growth at different ages.
I attach such a plot that we have produced, hoping that it will remind someone if they have seen or used such a plot in a publication.
Do captive Correlophus ciliatus require UVB? And are they thought to be nocturnal or crepuscular?
I am writing a paper on whether Crested geckos require UVB lighting as part of their husbandry, any help will be greatly appreciated.
I intend to begin live trapping efforts on a carnivorous lizard that has yet to be consistently trapped. Some efforts have been made to determine bait preferences with live traps, but successful trapping is so low and with no statistical significance.
Has anyone used baited camera traps to determine bait preferences? Would the animal need to consume the bait for it to be considered a success?
Two African lizards, Lygodactylus picturatus PASTEUR, 1964 and L. luteopicturatus (PETERS, 1870), are often kept as vivarium reptiles in Europe. As both taxa are similar in body size, shape and colouration, they are sometimes wrongly identified in the pet trade (especially the name L. picturatus is often used for L. luteopicturatus). Can anyone provide me good morphological characters which would be helpful in correct identification of these geckos? Or maybe based on any new (molecular?) data these names are just synonyms???
Thank you in advance for your help and answers.
I would like reference of this type of interaction in Anolis. The case that a male attack other male during the copulation
Being in Burma in 2011 in Mandalay City, I found a lot of agamas sitting on trees near the Kings Palace channel. These for my mind are from Genera Calotus, but when using Jacob Hallermann's key from 2000, I saw differences of this species from possible three species known from this part of Burma, as: C. emma, C. mystaceus and C. jerdoni. Moreover, colors of looking the same species males differ seriously. Is that just polymorphism, gender dimorphism or agamas can change its color and what kind is this species?
Hello Reaserchgate !
I'm a student in Heritage and I'm curently doing my traineeship in the science faculty of Toulouse where I inventory a lot of taxidermy.
I encounter difficulties to identify some of the animals and require your help.
Number 2 and 5 : I'm thinking about varanus dumerilii, but not quite sure.
Dimentions : number 2 is 37cm (long) / number 5 is 32cm (long)
Number 3 is 38cm (long) with the socle. Maybe an Uromastyx aegyptia ?
For number 1, I don't really know but it's 40cm long and 12 cm large.
Number 4 is 36,5cm long and 9cm large (with the socle), I don't know if it's just a lizard or a tiny monitor.
I can take more pictures of the details of course,
Thanks a lot !!
I have been running into an issue trying to stain lizard hatchling brains. The brains were fixed in PFA and then sunk in sucrose in a fridge. I am slicing at 50um in a cryostat and I mount the slices to the slides. I have tried gel coated and Superfrost ++ slides and gel coated is working better, but I am still losing over 50% of the slices during the staining process. I have tried letting them dry at 36C in an incubator and at 40C on a hot plate for 24 and 36 hours, and the hotplate is a little better then the incubator. I have also tried mounting the brain to the chuck with PBS and OCT, but have not found much effect of that (PBS might stay a little better, but with OCT we get much better slices).
I have also done adult lizard brains, before and did not have this issue, so I am not sure what I could try to do differently to get the slices to stay on the slide. I am doing the mounted staining because the slices are so small (diameter <0.4mm) and delicate that trying to float stain them either ends up with us loosing the slice or it becoming to damaged to use. Any ideas on what to try to get the slices to stay on the slide would be wonderful, as I am running out of ideas.
I would like to find out if cell cycle times in lizards are considerable slower (or faster?) than those in humans and mice.
Is Visual encounter surveys the only method?
Animals can't be trapped or captured in the area.
measurement of morphological details of the varanus species is not allowed.
I'm aware that the Mwanza rock agama (Agama mwanzae) can be found in the Serengeti National Park, Tanzania but I was also wondering if it occupied this area with any other sympatric species.
Could anyone help me in finding an identification key till species level of Gekkonidae family found in India? I will be too glad if anyone could send me an electronic file.
Molecular tests are often used to verify claims of captive breeding in birds of prey but I can't find anything similar for reptiles. Ideally the method would allow maternal individuals, eggshells and offspring to be linked unambiguously. Thanks for any help!
The thyroid glands from the common wall lizards were isolated and directly placed in Bouin's fixative. My question is for how long can the glands safely stay in the fixative before further processing?
I am trying to figure out if there is something that inactivates glucocorticoids in reptiles (fence lizards in particular) while the offspring is still inside the mother.