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Lipid Biomarkers - Science topic
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Questions related to Lipid Biomarkers
I am looking for a lipid biomarker of BBB leakage. There are recently published blood and brain lipidomics datasets but it is not clear which lipids are exclusive to the blood and if such lipids (in bound or unbound forms) are found in the brain parenchyma, can be construed as an indicator of BBB leakage (or transport across the BBB).
Which method do you think is more reliable and effective for the analysis of lipid biomarkers from the Precambrian? is cutting, hydropyrolysis or fluid inclusion assemblages analysis?
Dear colleagues;
I look for young scientists for authoring a book deals with subjects belongs to medical biochemistry. Are you interested?
A priority is to the English native speakers
Regards;
I was wondering about the role of free fatty acids in dinoflagellates, more specifically the symbiodiniaceae? What are the metabolic pathways and where are the different types of lipids usually manufactured?
Dear colleagues;
What are the novel and accurate methods for testing the following:
- Total cholesterol conc.
- Triglycerides conc.
- HDL-C conc.
- LDL-C & VLDL-C
- Lipid peroxidation marker (MDA)
Best regards;
This is the second time I failed to see red lipid droplets in my adipogenesis culture. Gene expression and lipid quantification already show that the differentiation is successful but no red droplets after staining. Any idea? I meticulously follow the protocol step by step, but still no success!
Can anyone help in finding out LDL for atomic absorption spectroscopy?
I have been using Anti-fade Prolong Gold but observed diffused bodipy florescence. I came across Mowiol-488 (Calbiobchem) and Fluoromount-G (southern biotechnology) has been used in papers. Which one I should go for or someone suggest me protocol for bodipy staining.
I have extracted DNA from suspended biomass collected on filters. Now I want to divide the OTU counts by the volume of water filtered for each sample, in order to abtain semi-quantitative abundance estimates. Does anyone know a reasonalble way to do it? I'm using the softaware packages "mothur" and "phyloseq". Thus far I only found the option to normalize all samples to one value. In my case, however, I have different values (volumes of filtered water) for each sample.
Thanks for any suggestions,
Yuki.
Any statistical analysis, eg, PCA or other indices, or the concentration of any compounds, eg, sterols and hopanols, may help to address the question.
Fatty Acid analysis using based FAME mixer Standard. but GLA(Gamma Linolinic Acid) is not in FAME mixer Standard.Prime Rose oil Slightly traceable GLA.This GLA in mother feed only. Please if any Standard for GLA in market give details and how to purchase that.
There are some reports about clozapine that clinical response is dependent on rise in the triglyceride levels.
We need to estimate lipid biomarkers contain in soil by GC MC. We have HPLC grade Methanol 99.9 % with water max. 0.05 %. Could we add solid KOH to Methanol without any additional treatment?
We are going to screen the some herbal drugs for its hypolipidemic effect. In this experiment, zebrafish (8 or 9 day post fertilization) must be stained with Oil Red O staining to visualize the lipids. If any one has a working protocol of Oil Red O staining pertaining to zebrafish, please share with us.
Thanking you in advance for sharing your knowledge.
Hi all,
I am looking for a ubiquitous cell membrane marker for murine intestinal tissue. Can anyone recommend one? For human tissue I have been using HLA-ABC, but didn't find an equivalent for mice.
Thanks.
I am studying the n-alkane from Paleosol and data of GC MS spectra showing 25C to 35C is dominated over the lower chain carbon with odd over even predominance. I am not able to explain the preference of higher length carbon chain in higher plants and lower chain in lower plants. The odd over even predominance is from terrestrial plants. Why is this?
On our high throughput hospital clinical chemistry analysers we measure haemolysis, icterus and lipaemia using simple spectrophotometric indices. This is to avoid analysing tests where these interference are know to lead to factitious results.
We have recently observed that some patients with persistent raised triglycerides have high haemolysis indices and there is a relatively strong correlation!
Has anyone else seen this? I am not sure if this represents increased invitro haemolysis in lipaemic samples or a direct analytical interference. The strong correlation suggests the later to me?
All help gratefully received.
Many companies providing single reagent solution for enzymatic reactions, by which we can quantify cholesterol, triglycerides, and HDL-C. Can I use the these human compatible reagents with rat or mouse sample? Is it okay scientifically? Thanking you in advance.
I am working with porcine cells and did live staining with green plasma membrane stain from lifetech. The results came out really bad.
I would be thankful to know if anyone has a suggestion other than green plasma membrane stain.
Recent studies suggest that there is still a benefit without significantly increasing side effects, but I'm not convinced that long term or in certain subgroups of patients, the metabolic change that we generate with very aggressive treatment is safe.
European recommendations about Lp (a) is to recognize it as FR CVD when its plasma concentration is above 50 mg / dl, whereas this value represents the 80th percentile in the population. Nonetheless studies to provide the 80th percentile value of 50 mg / dl, are from Denmark. In Spain (data from unpublished study DRECE) the 80th percentile corresponds to 30 mg / dl. How should we interpret these differences and their value as FR?
I'm trying to look at the uncategorized lipids out there, ones that might need to be analyzed for classification purposes.