Questions related to Lepidoptera
I have four female carolina sphinx moths as pets and one of them was unable to fuse her proboscis after emergence. Is it possible to help her repair her proboscis to enable her to continue to drink?
I need help from anyone that could confirm or correct the identification of the species listed in the manuscrito attached "Arsenura and Titaea (Lepidoptera: Saturniidae: Arsenurinae): new records for the Cerrado of Northeast Brazil "
Is it possible to determine the correction factor (Km) to estimate the (AED) for Lepidopteran species? Haven't found any literature that discusses the MRSD for Lepidopteran species. Would very much appreciate it if someone has any insight into it. Need to calculate the drug dose for Bombyx Mori.
Hello, can someone help me with an estimate of known species for Diptera, Coleoptera, Lepidoptera and Hymenoptera in these two territories? I have searched without much success, it seems as if all the papers I have reviewed shy away from offering a number of species.
Thanks in advance!
Does it act as a visual deterrent for predators or serves a purpose in the body's physiological processes?
Also, is there any similarity between the Sphingidae caterpillars and the larvae of Trilocha varians (Bombycidae) which also has a horn-like structure in the larval stage ?
Max Korb (1851-1933) was a German naturalist from Munich who collected mostly Lepidoptera and Coleoptera through Europe, the Middle East and North Africa but also elsewhere. However he centred much of his research in Spain and provided specimens to many contemporary naturalists who eventually described many taxa upon his findings, he never published anything himself but a comprehensive book on Central European butterflies.
A summary of eulogies on his life and work can be seen here (in German):
Nowhere is there any information available of the whereabouts of his collection, if there is actually one and hence my question. Most likely country would be Germany but other surrounding countries are a possibility.
Thanks for any help on this.
I've been collecting & planting seeds of butterfly host plants for the restoration program. And I need research references especially for tropical Southeast Asia native species (include all types of herbs, bush, or shrubs). I looking forward to having some recommendations from botanical experts.
In early 2022 I'll be completing a length field campaign, collecting Lepidoptera, Odonata and Hymenoptera in a very humid flooded forest in central Africa. Due to the nature of this work we will not be able to use conventional drying boxes, and will not have access to any electricity (we are not taking a generator for logistical reasons). What is the best approach to drying and preserving these specimens (which will number in the thousands) in the field?
Our proposed solution currently is a makeshift drying box with an air intake we will position over a fire, before storing in tupperware with silica crystal. However, this method is difficult to maintain and ensure equal and not excessive drying. One proposed solution is direct immersion in silica crystal, but this is not possible with delicate specimens which will later be analysed for pollen loads (contamination an issue). Alternatively, one could dry specimens in direct sunlight, perhaps under black tarpaulin, but our habitat type is not convenient for this. Drying is necessary (as opposed to e.g. alcohol immersion) due to other aspects of the research.
Entomologists have been collecting tropical insects for hundreds of years - what did Russell Wallace do?
I want to isolate the microbiom from the gut of the larvae but I have problems with the dissection. I tried to use needles and a scalpel from a dissection set for biologists but I had problems to open the larvae and to find the gut. Is there any trick?
I have no experience but interested to learn and apply mathematical models for insecticide resistance. It would be great if some colleague guide me from where to start. I have experience for insecticide resistance monitoring, isolines comparison, fitness cost analysis and genetics of resistance in some Lepidoptera insect pests like Spodoptera litura, Helicoverpa armigera.
Many thanks in advance
Hi, everyone, I am wondering what environmental variables will play an important role in butterfly and its endosymbiont? For example, land cover and growing degree days had the greatest contribution to Ecological niche modeling when Erin et al(2020) studied on Speyeria atlantis-hesperis (Lepidoptera: Nymphalidae) species complex. But I am more interested in endosymbiont. Are there any good suggestions on butterflies and their endosymbiont?
Thank you and hope to discuss with anyone who has good opinions.
Campbell E, MacDonald Z, Gage E, et al. Reconciling genomic and ecological species delimitation using a confusing group of butterflies[J]. Authorea Preprints, 2020.
Burlap traps are a way to mitigate the invasive Lymantria dispar dispar (tussock/gypsy moth) caterpillars, which defoliate mainly hardwood deciduous trees. Burlap is wrapped around trees and tied with twine, then folded to create a flap and ideal conditions where the caterpillars migrate into. The caterpillars are then disposed of in soapy water when the traps are checked.
If I want to study spatial ecology of these caterpillars, using quantitative analysis from each trap at a small lake surrounded by forest, how should I prioritize trap set-up (location, amount)?
Should the traps be completely randomized?
My study area is at maximum 2 square kilometres with a small Lake taking up about 0.25 of those square km.
Ideally I want to minimize confounding variables such as tree species the traps are placed on.
The goal of this project is to determine spatial distribution of the caterpillars and to mitigate them with weekly checks.
Any help would be greatly appreciated!
The risks of consuming dates infested by Ectomyeloïs ceratoniae Zell (Lepidoptera: Pyralidae)?
I would like to know is working in the field using LED light traps to monitor and to reduce populations of Lepidoptera pests in forest plantations.
I am interested in the size of Lepidoptera (moths and butterflies) and would like to know what is the smallest known species of this group of insects. It is probably a Nepticulidae (pigmy moths). The species in my figure below (unidentified) measures about 4 mm with the wings spread, and its dry body weight was 0.3 micrograms (0.03 mg).
We are generally more impressed by the higher figures (the oldest tree, the heaviest vertebrate…) than by the minima. Thus for instance one can read about the largest moths (Thysannia, Attacus: http://entnemdept.ifas.ufl.edu/walker/ufbir/index.shtml). However 'smallness' has interesting biological implications (see the recent book by A. Polilov 'At the Size Limit - Effects of Miniaturization in Insects'). I have seen descriptions of other nepticulids in the same range of size as 'my' species (around 4 mm: Dooren weerd et al.: http://onlinelibrary.wiley.com/doi/10.1111/syen.12212/full). Perhaps there are slightly smaller European species (some Stigmella spp., e.g.: http://lepiforum.de/lepiwiki.pl?Stigmella_Magdalenae).
So, does anybody know of any moth smaller than 3.5 / 4.0 mm?
Tachinidae flies are difficult to identification , so , I need classification key to the species which parasitoid on Lepidoptera>
In this year, I have joined a research team focusing on how to rear larvae and moth of durian seed bore (Lepidoptera : Noctuoidae), for example : Mudaria luteileprosa Halloway and Canogethes punctiferalis frequently found durian cultivated in Thailand, Malaysia and Indonesia, to be used for conducting a series of quarantine treatments on fresh durian. In initial steps, we need to collect massively those insects. Unfortunately, what we have seemingly efforted still far away to the target. We would highly appreciate if you could share the information about how to obtain those commercial mass-reared of durian seed bore and their artificial diets. Thank you very much before.
Hendra Adi Prasetia
I am trying to analyse the chemical profile of defensive droplets from the lepidopteran larvae (T Trypanophora semihyalina ). when i collected and tried to dilute in the hexane and acetone the secretion is getting gelly and it is not dissolving. So looking for a suitable solvent.
I can't find Rhyacionia duplana (HÜBNER, ) picture of female genitalia (Lepidoptera: Tortricidae). I need it for comparison and citation.
I am trying to find a topic for my PhD thesis. I've done two MSc; one in Landscape Architecture (about Ecological Landscape Design and Permaculture) and one in Forest Entomology (about Lepidoptera). Now I want to study further both of them in PhD. Can you help me find a topic that icludes both and woth studying.
The image was taken on June 21, 2020 in Hungary. To my knowledge, Brimstones mate in the spring after overwintering, not in the summer after they emerge from their pupae, but these two specimens have disproved all of my previous observations. May and most of June were colder than avarage in Hungary. Could that be the cause of this phenomenon? I couldn't find any information about this.
I want to determine the phylogenetic age of the members of the Lepidoptera (Sphingidae) based on mtDNA, using BEAST software; But I do not have a good indicator. Please what is mutation rate in the mitochondrial genomes of the Lepidoptera (in million years)?
What are some applied methods for controlling Zeuzera pyrina, the leopard moth, a key pest of walnut orchards?
Gardeners in Iran have recently had many problems with this pest. Current control methods include:
- Spraying with organophosphate synthetic insecticides like Diazinon for the control the first larval stage at the young bud
- Paste formulations for use at new holes (fumigation)
- Imported pheromones
These have been used, but have low effectiveness.
Good day, currently I am practicing extracting total DNA using drosophila melanogaster (fruit fly) adult as well as larvae as my sample with Qiagen DNeasy Blood and Tissue Kit. However, I keep obtaining DNA with smears.
My main purpose is to extract total DNA and send the extracted DNA for 16S Amplicon sequencing (V3-V4 region). The insect is from the Lepidoptera family and I will only be extracting DNA from the larvae (which is like caterpillar).
I followed this protocol:
1) Grind 50 mg tissues by disposable micropipette tips a 1.5 ml micro-centrifuge tube with added 180μl PBS
2) Add 200 μl buffer ATL + 20 μl of proteinase K and vortex and incubate at 56˚C for 1 hour
3) Add 200 μl Buffer AL + 200 μl absolute ethanol, vortex
4) Centrifuge 8,000rpm 1 min
5) Pipet the supernatant in DNeasy Mini spin column
6) Centrifuge for 1 min at a speed of 8000 rpm, Discard the collection tube and flow-through, place the DNeasy Mini spin column in a new tube
7) Add 500 μl Buffer AW1 and repeat centrifugation.
8) Add 500 μl Buffer AW2
9) Centrifuge at a speed of 12,000 rpm for 4 minutes
10)Place DNeasy Mini spin column in a 1.5 ml tube and add 50 μl buffer AE and incubate for one min at room temperature followed by centrifuge at a speed of 8000 rpm to elute the DNA
So my questions are:
1) Is there any suggestion why this happen or is there any method to decrease this degradation
2) How "intact" should the DNA be/how little degradation should there be to be sent for NGS?
I am building low-cost terrestrial emergence traps out of tomato cages and polyester no-see-um mesh netting. The traps are about 2 feet tall and have a white collection bottle at the top. I have a choice between black and white mesh netting. Which would be best? I have attached a picture of the prototype with white netting.
Does anyone have thoughts on the relationships of the Lepidoptera:Papilionidae genus Papilio L.? I personally believe that separating it out into Pterourous, Heraclides and other distinct genera is superfluous. If you agree with this "splitter" taxonomy, what are your justifications?
I appreciate any thoughts on this subject.
Avery R. Forrest
I have a question about Lepidoptera proboscis.
Despite having an idea, I wish to ask the communauty if there is a serious answer still existing.
1- why the butterfly proboscis is in two parts ?
I suppose it has something to do with embryology and ontongeny , it is a paired organ.
2- As for the attachment system of the two gutters, by means of hooks or a zipper, it also seems quite complicated. Is there a reason why there is not a weld that seems more hermetic and that would allow to have "one piece" ?
no real idea
I am working to associate a leaf mining caterpillar with a species of adult microlepidoptera. Does anyone have suggestions on approaches to DNA extraction for microleps? I have COI-5P primers from BoLD http://v3.boldsystems.org/index.php/Public_RecordView?processid=GMLC413-11
Semiochemicals communication is well studied and well developed in Lepidoptera, It was a mystery before first chemically identification of (Bombykol, 1959) that was not easy, well it was done by using half of a million insects.
Insect scales (especially moths body scales) also studied before and chemically identified and their role is also presented with context of behavioral studies. I want to observe one moth pest scales and their role in behavior studies, i have tried different ways to collect moth body scales, I am not sure either the method is correct or not.
It will be highly appreciated for suggesting me any method of removing moths body scales.
I mean sometime we have limited individuals concerned with any taxa, carrying risk to dissect or designated any type.. now a day few new techniques are available to scan even anatomy of any insect. its hopefully a best technique or methodology for future prospective if we work on this . suggestion are welcome.
Werny, K. 1966, Untersuchungen über die Systematik der Tribus Thyatirini, Macrothyatirini, Habrosynini und Tetheini (Lepidoptera: Thyatiridae). - Inaugural-Dissertation zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften der Matematisch-Naturwissenschaftlichen Fakultät der Universität des Saarlandes, Saarbrücken. pp. 1-463 Mit 436 Figuren.
Also, it would be of great help if you know to which insecticides oriental fruith moth (Cydia molesta) developed resistance?
I would like to do a comparative study between two type of light traps for the collection of Lepidoptera (moths). Both types are identical in design but differ in type of light source used. Trap A uses a standard 125W mercury vapor lamp, trap B uses a newly developed type of Led lamp.
In this study I would like to answer following questions:
· Is there a difference in abundance of moths captured by both traps?
· Is there a difference in species richness captured by both traps?
· Are certain families of moths more attracted to one lamp or the other?
Due to budgetary constraints I only have one trap of type B at my disposal. This, combined with my admittedly lacking background in statistics, is giving me a few headaches in how I should design the experiment.
So far my DOE is as follows:
· Both traps will be placed simultaneously in a homogenous habitat
· Traps are placed at such a distance so there is no light-interference
· Temperature, wind speed and humidity will be recorded every trapping night, right beside the traps (hopefully there will be no significant differences in these parameters between the two trap locations)
· After every trapping night, the traps will switch spots (by switching the traps every night, I hope to exclude the factor location as much as possible)
· Trapping will happen 2-3 times a week, for a period of 8-9 weeks, giving a total sample size of 16-27 catches per trap type. This number, however, is very dependent on the weather
· To avoid carryover effects, at least one night will be left in between trapping nights (i.e. trapping will never occur on two consecutive nights)
Can anyone give his professional opinion on this experimental design? Am I making any mistakes?
Is my sample size large enough?
Do I need already need to determine how I’ll process the data/what tests I will use at this point in time?
Thank you in advance.
It is very important publication to make inventory of morth and butterflies of the National Park "Alania". This work is in https://jbc.bj.uj.edu.pl/dlibra/publication/371910/edition/354398?language=en# but close for me.
A few days ago I found this weird thing on my Caligo memnon L4 caterpillars. When I touch them they lift their head up and project a small "inflatable" horn-like organ. It is similar to an osmeterium in papilionid caterpillars, but there is no smell. I also found the small slit on my L3 caterpillars, but there isn't any horn-like organ. Could anyone explain what this is?
I am going to be in Costa Rica and I wanted to put butterfly traps with rotting fruit as bait. But I've been told there are quite a lot of monkeys with stealing tendencies.
Even if I put other bait (dung, rotten fish... although I guess the atracted butterflies will be different), I am scared they'll steal mess with the traps or break them. Do you now any method to keep them away?
in many insect including Lepidoptera, there are two kind of sperm: Eupyrene and Apyrene. how can we distinguish two kind of sperm in pictures?
All these moths (Family Erebidae) were photographed from Bhutan. About 140 species were photographed, of which around 120 has been identified. I am looking for a person who can help me to authenticate my identifications and suggest ID for the remaining unidentified species. (Unfortunately, all the moths are in photographs as the voucher collection in Bhutan is illegal)
Now a day many publication on Lepidoptera published from reputed or renowned Journal by experts in the field of taxonomy on Moths. But they mainly focus on morpho taxonomy (mainly on external genitalia) but ignore wing venation. is there less importance of wing venation or due to other reason? waiting favorable response from dear experts...
Is there any analysis or explanation of why majority of agricultural pest species come from the orders of Coleoptera, Lepidoptera or Hymenoptera?
Any idea how to expose the cocoons with pupae in the wild without being prey to ants or other animals?
Are you aware of scientific works that illustrate the method?
Thanks in advance.
After reading these articles I was wondering what would be some primitive Lepidopterans that have Exarate pupae. I am familiar with most Noctuids and Erebids having adaptations to chew through the pupae but they do not have movable appendages (if we go by the definition of Exarate from the link). Is the article referring to the Noctuoidea superfamily as Primitive Lepidopterans ?
I also feel that the information on the Link might be slightly misleading, so please share better references if possible. They have listed only Butterflies under Lepidoptera, poor Moths get no attention yet again.
You asked me to send my paper entitled "Molecular phylogenetic relationship of African swallowtail butterflies, ...., to Japanese butterflies analyzed by DNA sequences ..." that has been appeared in the journal Lepidoptera Science Vol.68 (2): 46-52. I would like to send you a copy of the paper, if you provide me your mailing address and e-mail address through the following my email address.
Toshio Sekimura, Dr.
From January 2014 to December 2016, I conducted monthly field trips in three different areas of Dhaka city, Bangladesh to investigate how butterflies survive in an inner-city habitat. I have found that the species richness (I did not count the species abundance) is declining with time.
Presently, I have:
* monthly species-specific data of three continuous years (36 continuous months) of the three different areas
* Four environmental variables (temperature, rainfall, humidity and sunshine hours)
* Pollution level
* IUCN Red List status
* Land-use analysis of these three different areas
Now, I am really confused considering the proper way to link these pieces of information! Could anyone of you please share some ideas?
My question is how I can protect myself and my colleagues against the toxins Thaumatopein, because I get very large allergic reactions on my skin?
Could someone tell me, can I do insolation od silk fiber on other way?
I'm posting the nest of Thaumetopoea pityocampa Schiff .
I am attempting to isolate ovary from Helicoverpa armigera larvae. I can't find any pictures showing what it looks like during dissection. I know it is possible since there are several articles describing sequencing of gonad transcripts (ovary and testes). Any help? Ideally I would like to see some pictures of what the ovary looks like during dissection and where exactly it is located.
Thanks in advance!
EDIT: Thanks to Muhammad F Chaudhury for providing me the full version of his article titled " Spermatogenesis and Testicular Development of the European Corn Borer, Ostrinia nubilalis (Lepidoptera: Pyraustidae)". In it he describes that in this insect the testis of late-instar larvae are yellow-orange colored, reniform and aprox. 1mm in size.
This information allowed me to identify them in Helicoverpa larvae.
I have used graph eyepiece for the measurements and calculated graph division in different magnifications like in 4x= 0.25 mm, 10x= 0.1 mm and 40x= 0.025 mm. I have drawn maximum diagrams in 40x on the graph paper. kindly suggest me how to measure the reference line for each diagram like setae, crochets, body segments and head.
kindly suggest me how to measure the reference line for each diagram like setae, crochets, body segments and head.Thank you.
External genitalic attributes are important characters in Lepidopteran taxonomy to discern species as well as defining genus. There are two important papers that generally followed by me such as Klots (1970) and Maes, K. V. N. 1995.
Is there any other paper which deals with details of Lepidopteran genitalia nomenclature ? I wish to freely discuss which are important characters which are significant enough to discriminate species.
I want to examine the internal anatomy of Trichoptera and non-Ditrysian Lepidoptera larvae. The genus or species does not matter much for the first examinations, but it would be beneficial to know which species it is. Can anyone provide some fixed specimen? Only thoracic segments would also be fine.
I need advice from a specialist/taxonomist of Microlepidoptera who can give me more details on these specimens of Glyphipterix sp. See photos attached. Any references are good appreciated as well. Thanks for your help.
In the last decade, the population of butterflies has diminished in the colonies of hibernation in Mexico, so I want to know which are the impacts of the deforestation and what other consequences can have.
We are analysing the external morphology of adult moths, so we would like to check as many specimens as possible, mainly pests. If possible, we would also like to compare them with Trichoptera.
Could anyone send us some individuals (about 5-10, but many more would be much better) to Mexico? We would pay shipping.
I'm from Hochiminh city - Vietnam. One year ago, I and 2 others found a new pest in sugar which is Chilo tumidicostalis (Lepidoptera: Pyralidae) in Vietnam. Right now, I am getting some research in artificial food for sugarcane bore
[Diet 1= Kanta and Sajjan (1989); Diet 2 = King and Hartley (1985)
However, my food was sour and got a lots water. Every 3 days, we have to change food and it make me a lots times,
Do you know for doing simple artificial food for sugarcane bore? (less time and mass rearing Chilo tumidicostalis)
Or Can you help me for cooking process of artificial food?
Thanks a lots,