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Hi, I have a problem concerning my LIF measurements, intensity Vs concentration fails to be linear, also some dilutions have spectra lower than blank. Im working with a bacterial suspension about 10^8 cfu/ml. diluted from 10^-1 to 10^-10. also I tried another dilution sequence 1:10, 1:20, 1:40, 1:80, 1:160, 1:320, etc.... some dilutions have fluorescence intensity lower than blank which was normal saline. i need help, where am I going wrong.
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My guess is that light scattering is the problem. Bacterial suspensions scatter light, which is why they are turbid. (Turbidity is actually used as a standard measurement of bacterial cell density. This is known as the McFarland measurement and there is a special instrument for measuring it.) The denser the bacterial suspension, the more light scattering interferes with detection of the laser-induced fluorescence. At a high enough density, there will even be multiple scattering as another effect. I would expect LIF to give a linear result only for very dilute bacterial suspensions.
Additional confounding effects may arise from differences in the growth phase of the bacteria, their shape, their degree of aggregation, their species, and whether they shed any outer membrane vesicles or other scattering substances into the medium.
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I am working on laser moving heat source model in ansys thermal transient. I got the results like temprature variation of model over time. I need to measure melt pool depth by measuring the depth of isotherm of melting point of material from top and also the temperature gradient plot inside the melt pool. How can I get it?
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Ortamın sıcaklık değeri için Sıcaklık ölçerin yapıldığı malzemenin yapısına ve sıcaklık haznesinin içindeki sıvının cinsi ortama uygun olacak şekilde seçilmeli.
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On some samples I see this peak. It has nothing to do with the set up. I am using a 532nm laser. I think it could be correlated with the cleanliness of the surface. If the surface has a lot of particles on it, I get these peaks. This could be a false conclusion though. So far I have just found out that this peak could be due to Si-H stretching or sp1 C-C. Have you measured something similar? Is there an easy way to find out whether or not this is Si-H?
Thank you very much
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I wanted to show a drawing but I couldn't insert it.
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We have a LIBRA (Coherent Inc) femtosecond laser amplifier (1 kHz). Recently, the oscillator of the laser (Vitesse, 80 MHz) is not getting mode-locked. We also have a Mai-Tai oscillator (80 MHz) with output power of 3 Watt. The LIBRA amplifier requires <170 mW of power for seeding. The pulse width of Vitesse output is <100fs and the Mai-Tai output pulse width is 80fs. Can I try to seed the LIBRA amplifier with the output of Mai-Tai? Kindly give your opinion based on your experience in a similar situation. Thanking you.
P K Datta
Dept. of Physics
IIT Kharagpur-721302
India
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I am confused. The Mai-Tai has a slightly shorter pulse so it's bandwidth should be slightly greater than the Vitesse. Looking at the data sheet for the Vitesse, the specified bandwidth is >10nm (FWHM), but probably is not much greater than 10nm if the pulse width is 100 fs at a central wavelength of 800nm. A 30 nm bandwidth would support 25 fs pulses assuming a sech squared temporal profile.
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Im looking for a compact pulsed laser with 50-150 ns long pulses and prefereably 500-1000 mJ of pulse power. All Q-switched lasers I seem to find have 5-15 ns long pulses. (wavelength is not important). A MOPA fiber laser version can get me the pulse length right but not the power...
Any suggestions?
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Yes, high-power Q-switched lasers with 100 ns pulses exist, but they are rare. Custom solid-state or hybrid systems may be required.
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A lead-based perovskite single crystal was subjected to high-energy laser etching, and subsequent XPS spectral analysis of the etched region revealed the emergence of a loss feature peak (binding energy = 151 eV). Is the occurrence of this peak attributable to structural degradation of the crystal induced by laser damage? Does the intensity of the loss feature peak exhibit a correlation with the degree of structural deterioration? Could provide relevant references addressing this phenomenon?
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The loss feature peak in XPS spectra after laser etching of lead-based perovskites likely results from structural degradation caused by laser damage. The peak intensity may correlate with the degree of structural deterioration, reflecting changes in electronic structure or defect states.
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I'm trying to find a solution that would measure the mode characteristics as accurately as possible and not be particularly expensive. Direct detection of high-power lasers can easily damage the device. I think an attenuator might be needed, but since the output light is linearly polarized, it is possible that the general attenuation method will change the pattern of the laser. Is there a better way to measure the nanosecond pulsed laser characteristics?
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There used to exist Prometec Laserscope. Ages ago, I was using it for beam scanning of 2 kW CW CO2 laser. It is mentioned in the paper:
It is not suitable for pulsed lasers, unfortunately.
For pulsed laser, you may try a method I was using for pulsed YAG lasers. You need several 100% mirrors deposited on transparent material in such a way that the transmitted beam is not distorted. After the mirror, you have an attenuated laser beam with the power of about 0.5% from the incident beam.
You repeat mirrors as necessary. However, remember that the reflected beam should not return directly into the laser. The whole system must be skewed a little as to prevent damage to the laser and multiple reflections. Perhaps 45 degrees mirrors are the most convenient, safe and easy to use. On the path of the reflected beam, you need an absorber.
Attenuated pulse may be analysed by suitable methods. Hence, now your problem is reduced to low-power laser pulse analysis.
Every so often, an analysis of craters burned in an acrylic plate can be enough. It is quite accurate as long as the laser fluence is not too high, and using profilometers provides accuracy of crater measurement.
At first, you may try burning a crater with a beam which is not attenuated, and asses the results.
Maybe widening the beam with a lens will suffice.
Do not inhale the fumes from acrylic, they are poisonous.
Do not put the acrylic plate too close to the laser and optical elements.
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Dear Researcher
I am working with an Laser Doppler Vibrometer (LDV) to measure the natural frequencies and mode shapes of concrete bridges. While I have successfully applied LDV in the lab and on pedestrian cable bridges under controlled conditions, I now face a challenge when testing highway bridges.
🔹 The Challenge: For certain bridge tests, it is not possible to position the vibrometer aligned with the vibration direction. As a result, the measured velocity component is along the laser beam direction, rather than the actual vibration velocity. This misalignment introduces errors in the frequency and mode shape estimations.
Key Questions for Discussion:
-Who has experience using LDV for bridge measurements, particularly in real-world conditions where perfect alignment is not possible?
-What methods have you used to correct the measured velocity and convert it into the true vibration velocity?
-Are there practical techniques, such as angular correction formulas or software adjustments, that you have successfully applied?
📌 Your Insights Matter!
If you have worked with LDV for bridge monitoring or have expertise in overcoming angular deviations, I would greatly appreciate your input. Sharing your experiences, methodologies, or references to relevant studies will be highly valuable.
Looking forward to an engaging discussion!
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Ihtisham Khalid thanks for your response. about triangulation-based approach, Should I find the cosine factor correction for each point seperatyely?
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We need a photomask that can withstand high laser intensity.  We use a 248nm excimer laser to perform photomask patterning processing on materials.  The acting power on the photomask is 250MJ/cm², and the photomask needs to have long-term stability.
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There are three major laser mask-making techniques. The first is to directly remove the thin films of the photomasks (laser engraving). The second is to use laser beams as the light source to expose photoresists covered on the photomasks (laser-based lithography). The third is to generate patterns on the photomasks via photoresist (laser pattern generation).
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Dear forum users!
I have a question about the temperature in the Ideal gas equation when calculating the GHG flux, e.g. CO2 measured with LiCOR or Gas chromatography from the static chamber method.
I have found differences in the literature between them. So, my question is, what temperature should be used to calculate the flux?
Is it Air temperature, Inside chamber temperature, soil temperature, Laser temperature for LICOR/GC, or Laboratory ambient temperature for GC?
Thanks in advance.
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Thankyou Daba for such a detailed answer.
My question is more about the Temperature compensatin. Our calculation involves volume correction and Ideal Gas equation. Is it important to adjust for constant standard temperature for all measurements for example 20 C
Or it should bd corrected to the ambient temperature measured at sampling event. For example 273 + daily temp to individual measure
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Comparative outcomes of standard laser fistula closure (filac) versus flac combined with advancement flap in the treatment of complex anal fistula.
Could you please clarify whether suturing the internal opening was done in the standard FiLaC technique applied to the patients in your study?
The preliminary publication at Research Square states that the internal orifice was closed with a suture. However, nothing was mentioned in the paper published this month at Techniques in Coloproctology.
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there is a very low chance that one of the authors will see your question here...
I found that paper here on RG:
You may open that publication and add your questions as a comment to that publication...
Best regards
G.M.
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hello,
i've been using a spectra-physics Nd-YAG laser for the past 2 years working with the fundamental 1064 nm with each run about 2 hours. the power starts at 2.5 w and ends with about 1.3 watts. during the last week run, the laser spot died suddenly so i turned off the device and tried to run it again after 15 minutes. the laser spot starts well but it dies again after about 10 minutes. since then, the problem exists. can you please suggests any advice?
Ahmad
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Dear John
Thanks alot for your concern and advice, I've been through your sugestions and found nothing wrong for sure. It seems to be a soldering issue since the device started working again.
The decay in power persists till know, do you suggest buying new flashlamps? since the ones we have came with the device 21 years ago.
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in many laser amp system,
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Short answer: no.
The coherence length defines a distance over which if you know (or control!) the phase at location A the phase at location B is no longer related to the known phase at location A. The phase at location B wanders with respect to the phase at location A and averaging over a short time B goes through all possibilities. So at any given moment the difference in phase between point A and point B is random. Well, technically the coherence length is defined at a location where the phase isn’t quite yet completely uncorrelated but it’s getting there.
So, if you hold the phase of laser X constant relative to the phase of laser Y, but then you combine them at a point where their path lengths have differed by more than a coherence length, then their phases will no longer have a fixed relation. They aren’t coherent, and you won’t get the beam quality expected of coherent beam combining.
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Hi, I'm wondering how I can calculate the fluence of a laser from given parameters . like we have femtosecond laser with Bessel Beam .
1-160Mirowatt Power on power meter
2-10 Hz frequency
3-Beam spot diameter is unknown
4-50x with 0.6NA optical lens
According to the equation Fluence will calculate by F=E/A. But i am confused about Total power ,power on power meter ,pulse energy , laser spot diameter and the A in given equation. Is there any way to calculate the (fluence) of this laser from the mentioned parameters? what's the mathematical relation between given parameters
I would be truly grateful if anyone could answer me.
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No, I’m afraid that isn’t enough information. Fluence is energy per unit area, and you won’t be able to determine the fluence without determining the profile of the beam spot.
So, what energy and what area? Well, for average fluence, you could use any area you like. If you shine the laser at a 3x3 m wall, the average fluence on the wall is 16 uJ per 9 square meters. But, of course, that isn’t very interesting. You know most of that area is seeing no fluence, and there’s a much smaller area that is experiencing a much higher fluence. That’s an exaggeration, but even if you use an area roughly the diameter of the beam, that’s still an average and there will be portions of the beam that are producing higher fluence on a smaller size scale. What area you use depends on what you want to know and also on the size scale of features of the beam profile.
Usually people are interested in size scales smaller than the beam. For example there are physical processes that require a threshold fluence to get started. Others are nonlinear and are very sensitive to the peak power. So, maybe you want to know the highest fluence seen on a very small size scale. The interesting size scale might be determined by the process you are investigating. Or, if your beam profile is very smooth, (like for example a Gaussian) then there is a size scale over which the beam intensity doesn’t change much, so the integral over that area approaches the local value.
For whatever area you choose, you need to determine the fraction of the laser energy that passes through that area. It can be a great help if you have a known calculable beam shape. For example, if you know your beam is Gaussian, you can relate a measurement of a large part of the beam to the beam profile and then determine the fluence over any part of the beam by computation. Suppose you have a beam profiling camera. In the image you can find the centered circle that contains 87% of the total energy. That is the 1/e^2 diameter. The average fluence is 87% of the measured energy over the aperture. However, knowing the shape, you know more than that. You now can infer the whole profile. For instance the peak fluence in the center of the Gaussian is 2 X the average fluence in the 1/e^2 circle. Etc.
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I have some cells marked with a plasmid producing luciferin/luciferase.
If I check the cells for their luminosity, I get very little signal. In fact people use specific camera that get images with very, very long exposures.
However, if I check the same cells with a confocal microscope, where the cells are painted with a monochromatic laser, then I can see a signal rather clearly.
What is the physics behind this kind of 'enhancement'?
Can it be that the laser is exciting luciferin producing a higher signal than luciferin alone would?
Thanks
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Hi, Luigi. You may want first to try turning off your laser, cover all LEDs with black tape and darkening your room to use the confocal photomultiplier(s) as detectors for the bioluminescence signal (although I guess the lightpath will be larger than the optimal—too much signal loss)
About your question: In confocals the emission is detected simultaneously with sample irradiation. Did you somehow separate the excitation from emission reading?
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  • Hi! The SHG BBO crystal of our OPA system has detached from the holder, as shown in the photos attached. I want to adhere the crystal to the metal holder. I'm not very sure that whether I can use Norland UV curable optical adhesive NOA61? (I learned from the introduction of this adhesive that it can effectively stick glass optical components to metal surface.)
  • I plan to apply glue to the four corners of the crystal and fix it to the holder, and then use UV light to cure it. Can you please tell me is it the right operation? What should I pay attention to?
  • Thanks a lot for you support!
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Wow! Not much optical path length in that crystal!
I think your plan with the UV cure adhesive sounds fine. We more often use a thermally cured epoxy like Ablebond or Minbond, but we’ve had good luck with UV cured adhesive in less challenging uses.
There are just a couple things to consider. It doesn’t stick until the UV light hits it, so you’ll want perform the mounting in an orientation where gravity helps keep it in place until you cure the glue. A similar point: it doesn’t stick unless UV light gets to it. It doesn’t look like an issue here: it’s just a thin plate and BBO is transparent to the curing light. But, the point is, you want to be sure the curing light gets to the glue.
The one think that concerns me: why did it debond in the first place? Glued at the corners, there is little thermal conductivity. Do you think there might have been enough absorption in the BBO to cause any differential thermal expansion? This looks like a simple arrangement where that isn’t an issue, and SHG in BBO generally doesn’t deposit much heat. Nevertheless, it might have come unglued for a reason.
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Upcoming Events "CONFERENCES" in Berlin, Germany |October 24-26 2025.
Global Meet & Expo on Astronomy, Astrophysics and Space Technology (GMEAAS2025):
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Upcoming Events 'CONFERENCES' in London, UK | April 07-09, 2025. ANE Global Meet and Expo on Astronomy, Astrophysics, and Cosmology. https://astrophysicsmeet.org/
If you want to participate virtually, you can contact the manager by email to guide you.
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I have been using 785 nm continuous laser for 20 months. 350 mW is the maximum output of the laser mentioned by the manufacturer. At the beginning I used to get the same power with my power meter. However, I can see the power is 285 mW, which means 20% dropped from the maximum power. Is there any suggestions why the power is dropping? any thumbs of rules that can predict the laser life time ?
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May be your laser contains the hydrophillic component? May be the electric supplying of pumping system was slightly damaged (capacitors or transistors)? May be active media (or output mirror of cavity) slightly damages due to the dust in air and its adsorption on the surface of mirror. It is necessary to use air stream to realize processing of mirror. Check all the component of system or ask offices of company.
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A postgraduate student in pedodontics in India aimed to evaluate the diagnostic accuracy of the Diagnodent device for detecting early caries in school children. With a sample of 100 children aged 6 to 12 years, the student conducted a study at a local school. Each child underwent a clinical examination followed by a Diagnodent assessment, which uses laser fluorescence to identify carious lesions.
Suggest statistical solution for the above scenario &
Appraise how this study underscores the potential of integrating data science methods in clinical settings, paving the way for evidence-based dental practices?
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Çürük lezyonları tespit etmek, lazer floresanın gücüne bağlı. Bir de lezyonların nasıl yerleştirildiğine bağlı. Buradan elde edilen veriler kaydedilir.
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I run an imaging core with a Zeiss Elyra 7 doing SIM. I have a new user doing some experiments using fixed, isolated cells. For some reason, he's getting tons of photobleaching with the 642 laser (not present in the other channels). He's using AF647 conjugated antibodies, only .5% laser power/100ms exposure. The only thing I can think of that might help this would be adding mounting medium to his dishes (right now he's just got an imaging buffer). [Also, JUST had my preventative maintenance visit, so I know it's not a problem with our 'scope]
Would this help stabilize his fluorophores? What's the best way to add mounting medium to a 35mm coverslip dish? Is that actually a thing?
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I con not comment on the photo bleaching, but on the mounting of 35 mm glas bottom dishes (I assume that is what you meant with cover slip dishes). I have used WPI FD35-100 extensively and always used round cover slips (Thermo Fisher) with them. You/he should get some 1-2 mm lower than you inner plastic circle. Than I have cleaned them with some paper and applied a drop of mounting medium to the round coverslip. Than I turned them with tweezers, so that the drop is hanging and all bubbles go to the out side of the drop. Afterwards I just put the coverslip onto the glas bottom (without buffer just residual humidity) and it's even possible to push then the rest of the buffer and bubbles to the edges (with the back of the tweezers). He can have some test runs without the actual sample.
Best wishes Soenke
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Hello researchers,
I am constructing a PrismTIRF. I have a problem defining focus and detecting signals with labeled DNA (the bead sample works fine). I have looked for some possible solutions, yet have not been successful so far.
I wonder if the polarization type (p-polarized or s-polarized) of the laser can also cause this problem. Because I have read from some forums that a p-polarized laser can enhance the evanescent generation. My laser has a polarization ratio 100:1. Please share some experience with me.
Thank you.
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The polarization does not affect the strength or depth of the evanescent wave, but it does directly determine the polarization of the evanescent wave. See, for example, this animation.
The polarization of the evanescent wave can have a strong effect on how well the light interacts with the sample, particularly if the sample is ordered and the polarizability differs in different directions. However, even in an isotropic medium the existence of the interface itself breaks symmetry and changes the response depending on polarization.
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Zero stands for emptiness, for nothing, and yet it is considered to be one of the greatest achievements of humankind. It took a long stretch of human history for it to be recognized and appreciated [1][4]. In the history of mathematics considerable confusion exists as to the origin of zero. There can be no unique answer to the query, "Who first discovered the zero?", for this may refer to any one of several related but distinct historical issues† [2]. A very explicit use of the concept of zero was made by Aristotle, who, speaking of motion in a vacuum, said "there is no ratio in which the void is exceeded by body, as there is no ratio of zero to a number” [3][2]*. He apparently recognized “the Special Status of Zero among the Natural Numbers.”
If we believe that zero is explicitly expressed mathematically, whether in number theory, algebra, or set theory, is the meaning of zero also clear and unified in the different branches of physics? Or can it have multiple meanings? Such as:
1)Annihilation——When positive and negative particles meet [5][6], e+e-=γ+γ',the two charges disappear, the two masses disappear, and only the energy does not disappear or increase; the momentum of the two electrons, which was 0, now becomes the positive and negative momentum of the two photons. How many kinds of zeros exist here, and what does each mean?
2)Double-slit interference—— The interference pattern in Young's double slit experiment, what exactly is expressed at the dark fringe? And how should it actually be understood? For light waves, it can be understood as the field canceling due to destructive interference and presenting itself as zero. For single photons, single electrons [7], physics considers it to be a probabilistic statistical property [12]. This means that in practice, at the dark fringes of theoretical calculations, the field will also be likely not to be zero‡.
3)Destructive interference——In Mach–Zehnder interferometer [8],there's always been a question of where the energy in the destructive interference arm went [9]? There seems to be an energy cancellation occurring.
4)Anti-reflection coatings——By coating [10], the reflected waves are completely canceled out to achieve the purpose of increasing transmission.
5)Nodes of Standing Waves——In optical resonant cavity, Laser Resonator. " The resonator cavity's path length determines the longitudinal resonator modes, or electric field distributions which cause a standing wave in the cavity "[13]. The amplitude of the electromagnetic field at the node of the standing wave is zero, but we cannot say that the energy and momentum at this point are zero, which would violate the uncertainty principle.
6)Laser Beam Mode——The simplest type of laser resonator modes are Hermite-Gaussian modes, also known as transverse electromagnetic modes (TEMnm), in which the electric field profile can be approximated by the product of a Gaussian function with a Hermite polynomial. TEMnm,where n is the number of nodes in x direction, m is the number of nodes in y direction [14].
7)Nodes of the Wave Function——Nodes and ends of the Wave Function Ψ in a square potential well have zero probability in quantum mechanics‡ [11]。
8)Pauli exclusion principle—— Fermions are antisymmetric,Ψ(q1,q2)=-Ψ(q1,q2), so Ψ(q1,q2)=0;Here a wave function of zero means that "field" is not allowed to exist, or according to the Copenhagen interpretation, the wave function has zero probability of appearing here?
9)Photon——zero mass, zero charge.
10)Absolute vacuum——Can it be defined as zero energy space?
11)Absolute temperature 0K——Is the entire physical world defined as a zero energy state except for photons?
12)Perfect superconductor—— "The three 'big zeros' of superconductivity (zero resistance, zero induction and zero entropy) have equal weight and grow from a single root: quantization of the angular momentum of paired electrons" [15].
13)......
Doesn't it violate mathematical principles if we may interpret the meaning of zeros in physics according to our needs? If we regard all zeros as energy not existing, or not allowed to exist here, does it mean that energy must have the same expression? Otherwise, we cannot find a unified explanation.
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Notes
* Ratio was a symmetrical expression particularly favored by the ancient Greeks.
† Symbols(0,...), words (zero, null, void, empty, none, ...), etc..
‡ Note in particular that probability itself is defined as a probability, not an exact value. For example, a probability of 0.5 can occur in physical reality as 0.49999999999, and it is almost never possible to have an accurate probability value such as 0.5. This means that there is no probability value that never occurs, even if the probability is theoretically 0. It is against the principle of probability to assume that a probability of zero means that it will never occur in reality.
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References
[1] Nieder, A. (2016). "Representing something out of nothing: The dawning of zero." Trends in Cognitive Sciences 20(11): 830-842.
[2] Boyer, C. B. (1944). "Zero: The symbol, the concept, the number." National Mathematics Magazine 18(8): 323-330.
[3] the Physics of Aristotle;
[4] Boyer, C. B. (1944). "Zero: The symbol, the concept, the number." National Mathematics Magazine 18(8): 323-330.
[7] Davisson, C. and L. H. Germer (1927). "Diffraction of Electrons by a Crystal of Nickel." Physical Review 30(6): 705-740.
[8] Mach, L., L. Zehnder and C. Clark (2017). The Interferometers of Zehnder and Mach.
[9] Zetie, K., S. Adams and R. Tocknell (2000). "How does a Mach-Zehnder interferometer work?" Physics Education 35(1): 46.
[11] Chen, J. (2023). From Particle-in-a-Box Thought Experiment to a Complete Quantum Theory? -Version 22.
[12] Born, M. (1955). "Statistical Interpretation of Quantum Mechanics." Science 122(3172): 675-679.
[13]
[15] Kozhevnikov, V. (2021). "Meissner Effect: History of Development and Novel Aspects." Journal of Superconductivity and Novel Magnetism 34(8): 1979-2009.
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Let a bit continue.
So, in principle, really there can be some “absolute vacuum” – empty “Information” Set, however that is absolutely fundamentally impossible – information absolutely for sure cannot be non-existent, and so the Set exists being non-empty absolutely always – “in absolutely infinitely long time” having no Beginning and no End;
- and only concrete empty sets that relate to concrete informational patterns/ sets of the patterns can exist.
In this thread a concrete informational system/set of informational patterns/systems “material objects” “Matter” is considered, and in this case the general empty sets – or “vacuums” can exist as:
- “absolute vacuum, that was till the conserve can “There is no informational system “Matter” in the Set” was opened by some energy, let - 13.8 billions of years ago, when the first Matter’s FLE was really created, and
- “matter vacuum” when only the first version of the FLE-lattice was created and existed till at the inflation epoch, i.e. though the quite material objects “FLEs” existed, but “matter”, i.e. observed now a huge number of particles didn’t.
That’s all, after in the first FLE-lattice version corresponding a huge energy portion was pumped, and so the particles, which are some disturbances in the lattice, were created, no “vacuums” exist;
- including there are no such things as “vacuum where existed created/annihilated “virtual particles and fields”, in Matter only real particles, and real the Forces mediators/real fields, which constantly and always are created by their charges, exist.
Cheers
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Hello! The laser intensity in the source is displayed in amperes (on its screen), and my goal is to achieve a light irradiation of 100 mW/cm² given that the irradiated area is 0.125 cm², and the distance between the source irradiation and the irradiated surface (area) is 3 cm. So what current (in amperes) should I set my laser source on?
Thank you in advance.
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Hello! Here is the datasheet Tawsif Ibne Alam Gerhard Martens
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How do we measure lifetime using continuous laser and PMT detector?
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*Fluorescence Lifetime Measurement (FLIM)*
Principle:
1. Excitation: Diode laser emits continuous wave (CW) light.
2. Fluorescence: Sample emits fluorescence in response.
3. Lifetime measurement: Detect changes in fluorescence intensity over time.
Setup:
1. Diode laser (cw)
2. Sample chamber
3. Photodetector (e.g., PMT, APD)
4. Data acquisition system
Measurement steps:
1. Illuminate sample with cw diode laser.
2. Detect fluorescence signal.
3. Measure fluorescence intensity decay.
4. Analyze decay curve to extract lifetime.
*Lifetime calculation:*
1. Exponential fitting: Fit decay curve to exponential function.
2. Lifetime (τ) calculation: τ = 1 / (decay rate)
*Advantages:*
1. High sensitivity
2. Fast measurement
3. Non-invasive
*Applications:*
1. Biomedical research (e.g., protein binding, cell imaging)
2. Materials science (e.g., quantum dots, nanocrystals)
3. Photovoltaics (e.g., solar cell efficiency)
*Diode laser specifications:*
1. Wavelength (e.g., 405 nm, 633 nm)
2. Power (e.g., 10 mW, 100 mW)
3. Stability (e.g., <1% intensity fluctuation)
*Common fluorescence lifetime ranges:*
1. Biological samples: 1-10 ns
2. Organic materials: 1-100 ns
3. Inorganic materials: 10-1000 ns
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Let say I have a Mode-locked linear cavity fibre laser with 3m meter PM980 used for connect components within the cavity. Also, I am using a chirped fibre bragg grating (CFBG) for dispersion compensation.
PM980 has GVD of 0.014 ps^2/m
CFBG has D parameter = 0.42 ps/nm and reflection bandwidth of 9nm
laser pulse has FWHM width of 6nm
My first question is:
how to convert ps/nm of CFBG into ps^2/m?
is it simple as using β2​=−2πc \ λ^2.D​ (since D is given in ps/nm, do I need to multiply it with pulse's bandwidth or CFBG bandwidth?)
Second question:
PM980 used within the cavity is 3m. Since in a linear cavity round-trip length is calculated as 2L, therefore, to calculate total group delay dispersion, one should multiply 2 x 3m ?
Thanks in advance!
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Nanometre birimi metreye çevrilir ve piksel cinsinden karesi alınır. 2L olarak hesaplamanın nedeni mod kilidi 2n (tekrarlama) nedeniyle, n-1 ve n-2 tek ve çift girişimi sıralaması için yapılır.
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I have two different populations: one expressing GFP and the other one expressing mcherry. I analyzed them individually (as well as a negative control) in the cytometer and I did compensation. However when I mix both populations a huge amount of events are positive for both GFP and mcherry. I thought maybe more than one bacteria were being exposed to the laser at one time so I diluted and lower the number of events per second. However the result seems to be similar. Conjugation when I mix both populations is discarded. Has anyone else had the same problem and could help me?
Thanks in advance
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Thanks for the answer Hüseyin!
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Following laser and source replacement of one of our Bruker Tensor 27 units, the calibration peak from the laser path through the empty chamber is registering a good amplitude, but it is far out of the normal range. I'm seeing a peak position at ~65000, when it should be between 58000 and 62000.
Is there any way to fix this? So far, tweaking the interferometer position only decreases the signal amplitude and does not alter the peak position coming in. The laser position in the holder seems fine. Any help would be appreciated.
Failed OQ and image of the display attached.. Thanks!
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Removing the interferometer block was an unnecessary and dangerous way to resolve this issue. The solution was quite simple.
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seti or search of intelgenece articial nee to be fixed due to masss solar systems outhere thta expelled laser beam light ollimated wel beign so is neede to modificated the seti in a new device of armenta velasquez like radi ho can detect the laser signals
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SETI (Search for Extraterrestrial Intelligence) does not need to be fixed exclusively on radio-collimated signals, but radio waves are a primary focus because they can travel vast distances through space with minimal interference. However, SETI also explores other methods, including optical and infrared signals, to broaden the search for potential extraterrestrial communications.
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When Er-doped materials are pumped by 980nm laser, they can emit the light at 1550nm as well as green light due to the energy transfer between different energy level. When all the emission energy come from the pump light, the up-conversion green light should be suppressed in order to maximize the emission energy at 1550nm which is the important for optical amplification at telecommunication wavelength. The question is thus that, how to suppress the up-conversion emission in Er-doped materials and therefore most of the pump energy can be converted into 1550nm?
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spektral fitreleme kullanarak yeşil emisyonu bastırmak için kullanılabilir, böylece istemediğimiz emisyonları optik yolda filtreleriz. Farklı olarak katkılama işlemleri yaparak iyonları doğrudan erbium iyonlarına aktarmak yerine daha verimli şekilde pompa enerjisinin 1550nm emisyonunda kullanımı sağlanabilir. Ayrıca yukarı dönüşüm verimliliği sıcaklıkla düşürülebilirliği ele alınabilir. Çalışma dıcaklığı kontrol edilerek yeşil emisyonu azaltılabilir ve 1550 nm ye olan enerjiyi yenlendirebiliriz R.P. Wang
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Hi, I'd like to know if someone knows about a company where I can buy an affordable laser-scriber for perovskite films and another thin film for the fabrication of modules.
Many thanks,
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Hi Rodriguez,
UW LASER is engaged in laser-scriber for the photovoltaic industry.
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i am unable to interpret why its increases in start as shown in figure
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Mesafe arttığı için lazer ışık kaynağının da uyarma etki süresi uzar ve zamanla aynı sürede aynı miktarda etki edemez.
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I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
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Thanks Mike Albert for your detailed and informative answer.
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I heard that the intensity-dependence on the PL of the one-photon absorption is generally linear.
However, the intensity of PL at two-photon absorption increases to the quadratic power of the laser intensity. And three-photon does third power of the laser intensity.
Can you teach me a theoretical explanation?
Thank you.
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Try to check this one, if you need very brief theory:
Ma, ZC., Chen, QD., Han, B. et al. Measurement of Two-Photon Absorption Cross Section of Metal Ions by a Mass Sedimentation Approach. Sci Rep 5, 17712 (2016). https://doi.org/10.1038/srep17712
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I would like to use biofilmQ to analyse confocal laser image, how can do?
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Thanks you!
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Sir,
I want to work on the  laser assisted machining (turning) of Nickel Based Super alloy. Please help in finding the laser assisted machining facility in India.
Thanks and regards
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Thank you for the information sir
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I have a problem, in additive manufacturing (here specifically LPBF), if you make parts with different shapes through a fixed printing process, the parts will deform due to different geometry resulting in different stress concentrations. However, for the same shape of the part, the use of different printing process parameters to manufacture the part, will have an impact on its deformation? Such as different laser power, scanning rate, etc. (of course, if the part can be successfully manufactured)
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Hi,
Yes, of course, this issue was discussed in section 4.2 of this paper:
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Hello everyone, without going too much into details, my thesis is about improving the bonding strength at the wire bonding process. I should focus on laser pretreatment of the substrate. Has anyone some usefull knowledge/recommendations of literature etc?
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Makara ve ip gibi basit makineleri düşünebilirsin. Makaranın yükü arttıkça uygulanan kuvvet ve ipteki gerilim artar. Buna göre lazerin gücü arttıkça yapışma mukavemeti artar. Alt tabaka tüm yükü taşır ve sağlam olmak zorunda. Telin bağlama gücü, alt tabakanın oluşumu sırasında lazer tarafından uygulanan güce bağlı olur.
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I have a CVD-prepared material. The problem is that the LASER covers a smaller area than the islands, so I see localized effects. Sometimes, the intensity of the silicon peak is larger than the sample, and sometimes, it is otherwise. How do I deal with this?
I really appreciate any help you can provide.
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Likely you're using a Raman microscope for your measurements. Try to use the lowest magnification objective, this should increase the spot size.
(In the limiting case, you could try to completely remove the objective (just unscrew it) and excite the sample with the unfocused beam. Though the collected signal could become very small in this case.)
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I am working on a laser-based communication system for underserved areas, where a solar panel is used as a receiver and a laser as a transmitter. However, I am facing challenges in achieving the required signal strength due to limitations in laser capacity and solar panel conversion rates. I am exploring the possibility of modulating the laser signal onto sunlight to amplify the signal. Has anyone explored or achieved success in modulating laser signals onto sunlight? Are there any theoretical or practical limitations to this approach? Any insights or suggestions would be greatly appreciated.
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Güneş enerjisi saf fotonlarla elde edilebilecek en verimli ve zararsız enerjiye sahiptir. Bu nedenle heryerde kullanılabilir. İletişimde ise yaydığı zararsız dalgalar ve ışınlar nedeniyle kullanılabilir. Hatta iletişim fotonlarla sağlanabilir.
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Is it possible to detect microplastics in water through laser beam exposure?
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Yes, it's possible. Laser-based techniques, like Raman spectroscopy and laser-induced breakdown spectroscopy (LIBS), have been used to detect microplastics in water samples. These methods utilize the interaction between laser light and the chemical composition of the microplastics to identify and quantify them.
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I would be very thankful if somebody helps me with some practical advices about producing twin photons?
I know that twins are produced after a laser ray is directed on a BBO crystal. After the BBO there are two rings of photons and the twins are in the intersection of the rings.
1. I wonder in order to get the two rings must I have the laser ray falling strictly at 90 degree to the BBO? My crystal is very small 3x3 mm so I intend to focus the laser on it which means I would have different angles of them on the BBO so I fear I would not get the rings but a smeared spot. Is this right and what to do? I would like the beam to fall on a point but to be perpendicular. Is there a way to do this?
2. I am not at all sure how to capture the light from the intersection of the rings where the entangled twins are. I will filter the incident beam 405 nm by a filter at 405 but how to collect only the intersection spots to proceed and to direct them to a beam splitter b.e.? To make a mask with two openings? Or fibers?
Thanks in advance.
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Thank you Harri
I supposed it would go as you describe. Good to hear it by experimentalists. I am a little afraid that when using a mask (fibers even more) diffraction can spread my rays but I suppose it would be negligible as the holes would not be to narrow but at 1-2 mm.
Regards: Ilian
PS. By the way what is the easiest practical way to rotate polarization from V to H. Can you recommend a rotator of polarization? Of course sugar water solutions can do but I look for something more compact.
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When using different laser energies, what is the effect on the size of nanoparticles? Does it increase or decrease with increasing energy? Please help
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Nanoparticles produced by ablation are dependent on laser energy. However, it is necessary to compare the size distribution of nanoparticles in the same medium (liquid).
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because I prepared magnetite by sol-gel method, I got XRD peaks, but for Raman, I didn't get a peak at the range 600cm-1, should I change the wavelength of the laser, or what do I have to do
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Dear Wedad A. Alwesabi , thanks for your interesting question.
Raman spectra are not dependent on the excitation laser wavelength, sometimes we choose a laser with a wavelength or another to avoid interferences casused by luminiscence, which can mask the Raman bands.
Note, it is always useful for us in order to help you, to know what excitation laser you used and what settings did you choose to obtain your Raman spectra.
Magnetite has five Raman active modes, applying group theory to its spinel structure, according to literature these vibrational bands appear at around: 193, 306, 450-490, 538, and 668 cm-1.
But it happens that two of those five modes (193 and 450-490 cm-1) are really weak, and often they are not reported in many cases, specially when the sample is measured at room temperature and for nanoparticle samples.
Bands at 306 and 538 cm-1 are weak but often they can be seen in a Raman spectrum of magnetite. The more strong band is the one appearing around 668 cm-1, this one is the more characteristic or distinctive of magnetite.
However you cannot find this band in your experiment and from your XRD you know you actually have magnetite.
May be the answer for this question is related to the thermal stability of magnetite, which when heated in air transforms into maghemite and finally into hematite. These transformations can be induced by the laser power used during Raman measurements, due to energy transfer and heating in the sample under the laser spot. Raman spectra of maghemite and hematite do not contain the band at 668 cm-1 characterist of magnetite, while some bands from these materials could appear near the other two bands of magnetite, what together with the XRD results could cause confusion.
Several articles used the laser power variation to observe these transformations.
So it would be a good idea to repeat your Raman experiments with a fresh sample of magnetite and a low laser power. You could start with the lowest possible on your spectrometer and rising it step by step untill you get a good Raman spectrum of your sample.
Here you can find some articles about the topic:
Hope you find this answer helpful, if you need some extra explanation, please don´t hesitate to contact.
Regards
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I have a fluorescent probe that I'd like to test using flow cytometry, so that I can quantify its relative binding to different cell types. Unfortunately, the flow cytometer at my institution does not have a laser that reaches its recommended excitation wavelength. It does have a laser which overlaps its excitation spectrum, but only at a point which corresponds to 13% of the maximum excitation potential.
If I use this machine on samples treated with this probe is it possible that I can pick it up if the signal is strong enough, or am I risking inaccurate results?
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If it only reaches 13% of your maximum excitation potential, you won't get a good excitation signal, you are risking incaccurate results.
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The non-linear response of the electron in the driving laser field, we always mention in solids, inter-band polarization, and coherent intra-band dynamics of the electron. I want to deepen my understanding of the coherence related to the electron dynamics in the intra-band.
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Bant içi yapısını malzemenin yapısı ve atomik yapısı hakkında bilgi sahibi olduğunuz kadar hayal edebilirsiniz.
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In fact, there is little evidence about this, but it seems that different types of laser light are produced by stars under different conditions.
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Samanyolunda farklı türde ışık kaynağı var. Yıldızlar bu ışık kaynaklarından bazıları. Yıldızların bir kısmı kendiliğinden ışık yayar, bir kısmı ise diğer ışık kaynağı yardımı ile yayar. Işık kaynağı yardımıyla parlayan yıldızlar zamanla sönebilir veya kendiliğinden ışık yayan yıldızlar uzaydaki büyük olaylar sonucunda yok olabilir. Bu parlama ve sönme aşamasında alfa, beta, gama ışınları yayabilir. Kendiliğinden ışık yayan yıldızlar ise patlama sırasında ise x ışınları, radyo dalgaları, alfa, beta, gama gibi ışınlar yayar ve uzay ortamında farklı etkiler bırakır.
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Hello everyone,
I have already applied the Gaussian heat source and the next step is to apply recoil pressure in order to make the Keyhole.
I have read many papers but still not feeling confident to apply the recoil pressure. I would really appreciate If someone can explain the modules that I need to use for recoil pressure and some important settings in COMSOL.
Thank you.
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electron beam welding provision is there or not in comsol multiphysics
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Could anyone analyze about double passing of the laser beam through the febry perot etalon? Does it have negative effect on tuning the beam?
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Lazer ışınının çift geçişi, eğer ışın miktarını etkilemeyecekse herhangi bir sorun olmaz.
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The reason I suspect that the beat note should not be broad linewidth (~100 MHz), as I see in the spectrum analyzer, is because, using those same lasers, we can create a magneto-optical trap. Therefore, the laser's linewidth should be less than 5 MHz (Cs D2 natural linewidth).
What is that I am doing wrong? What parameters should I check to mitigate this noise?
Thanks.
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I can English a little bit.
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What factors or physical mechanism will affect the laser produced plasma (LPP) expansion process. How to reduce the instability of multiple measurements?
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The instability in the process of laser plasma expansion arises from a multitude of factors that influence the dynamics of the expanding plasma. Hydrodynamic instabilities, including Rayleigh-Taylor and Richtmyer-Meshkov instabilities, emerge at the interface between the laser-produced plasma and the surrounding medium, leading to mixing and irregularities in plasma expansion.
Additionally, self-focusing effects can occur due to the nonlinear response of the plasma to intense laser fields, resulting in filamentation or channeling within the plasma. Nonuniform heating, whether from variations in laser intensity or material properties, can cause uneven expansion velocities and density gradients, triggering further instability. Collisional effects and the presence of magnetic fields also contribute to complex plasma dynamics, altering expansion behavior and stability. Radiative cooling further complicates matters, as it affects energy balance and can lead to fragmentation or condensation of the plasma. Moreover, pulse-to-pulse variations in laser parameters or target conditions introduce stochastic fluctuations, exacerbating instability.
Understanding and mitigating these instabilities are vital for applications such as laser fusion and plasma-based accelerators, necessitating a combination of theoretical modeling, numerical simulations, and experimental diagnostics.
Resources such as scientific journals like "Physics of Plasmas," "Journal of Applied Physics," and "Physical Review Letters" provide valuable research articles on laser plasma dynamics and instability mechanisms, while textbooks like "Fundamentals of Plasma Physics" by J.A. Bittencourt offer comprehensive insights into plasma behavior and instabilities. Additionally, research groups and laboratories specializing in laser plasma interactions, such as the Lawrence Livermore National Laboratory's National Ignition Facility (NIF) or the Max Planck Institute for Plasma Physics, conduct experimental studies and provide valuable data for understanding and addressing plasma instability challenges.
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What is the relationship between the measured polarization rotation of far-detuned laser and the spin state of the atoms in Faraday effect?
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The polarization rotation angle of linearly polarized light is proportional to the spin projection along the propagation direction of the light.
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A 532 nm laser probe with a 5 mm lens tube means, the laser can be focused at 5 mm distance from the lens tube. Similarly what I can infer from the -20 mm lens tube?
Thanks in advance.
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Ah. Ok. So the meaning of the focal length of a positive lens is clear, right? Parallel rays coming from the left are bent by the lens towards the axis and all meet at a point to the right of the lens called the focus. The distance between the lens and the focus is the focal length. You can think of that focal point as a “real image“. Those parallel rays from the left are from some distant point source. Those rays coming together at the focus are like a new point source. If you continue to they right it would appear to anybody to the right of the focus that all the light is coming from that focus. In fact, if the distant object is not a point source, but something big enough to resolve (say, the sun for example), there will be an image of the object formed at the focus, and, again to anyone to the right of the focus it will appear as if the light is coming from an object (for example a tiny sun) located at the focus.
With a diverging lens the rays are bent away from the axis and they never come together at a real focus. However, if you trace the rays backwards, it looks as though all the rays are coming from a point to the left of the lens. The distance between the lens and this virtual source point is the focal length.
In either case, someone looking from further to the right will see the light as coming from a source located at the focal point. In the positive lens case the light will seem to originate (and actually will originate) from a point to the right of the lens. In the negative lens case the light will seem to originate from a point to the left of the lens. If we take the lens axis to be a spatial axis with the lens at zero and the downstream direction (to the right in my example) as positive, then a positive lens makes a real image on the positive side of the lens and the negative lens makes a virtual image on the negative side of the lens.
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When I was doing Raman spectroscopy, I observed that for the same sample (thin film), using two different laser sources gave different Raman spectra. We know Raman Shift is materially dependent property.What could be the reason for difference in Raman spectra?
Laser sources were the He-Cd laser (λ=325 nm), i.e., UV light source, and the He-Ne laser (λ=633 nm), i.e., visible light source.
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Fairly common effect. Often due to resonance issues. Take a look at graphene Raman spectra - well documented changes with excitation frequency. However, it doesn't look like you have much change in the figures that couldn't just be sample prep issues.
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Hi,
We need to buy a laser that is centered at 700-800 nm range (780 nm for example), has high output power (>100 mW), tunable (at least couple of nanometers), and has a linewidth preferably <1 MHz (not instantaneous linewidth). We got an offer from Toptica, but their delivery time is 32 weeks which will not work for us. If you know any other company that can deliver such a laser, we would be happy to hear.
Thanks so much
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Dear Leonid, thanks a lot. We will have a look at their products.
best
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Would it than be possible to change the position of this beam systematically?
Would the position of the detection depend on the position of the beam left from the double slit plane?
Would the sum of all measurements result in the same known pattern of a double slit if all positions had been used for the same period of time?
I have added a file with an image to illustrate what I mean.
The answer could be very helpful in figuring out the path of photons near the slit. I have done some investigations, documented in
But the central question has not yet been answered: Is there a random effect in the double-slit experiment or not?
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I've just updated the document. The normalization is corrected now. Before there was a deformation of the photons' paths.
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Hello,
I was measuring my sample - cellulose impregnated with polyethyleneimine, on a Raman microscope that has back-illuminated CCD. I used 633 nm excitation laser. In my spectrum, I got wavy fringes (due to interference?), but I don't know what causes them. I thought the etaloning effect was prominent only when using NIR laser, but I got the same results using 633 nm, 532 nm, and 455 nm laser (and not with 780 nm or 785 nm).
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What is your raw cellulose look like in the same wavelength range?
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In literature I found different laser efficiency values starting from 30-45%, do you think it's due to the varying materials, metal powder quality, printing environment or the difference in efficiency is due to different brands/marks of metal 3D printers?
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Absolutely, it depends on the inherent properties of the material used, such as reflectivity, thermal conductivity, and absorption coefficient, which play a major role. Metal powder characteristics, like particle size and surface morphology also impact efficiency. A, rougher surfaces can scatter the laser beam, reducing its efficiency.
While the printer brand may have a less impact on efficiency. If the printer is well-calibrated for the specific material, it's still a contributing factor.
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I want to know whether the temperature of the optical film must exceed the melting point of the material when it is ablated by laser.
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Depending on the wavelength of the laser and absorption characteristics of the film, damage may occur even before apparent heating and melting take place
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I want to use the laser dissection function of the Zeiss PALM microscope. We have PEN membrane Petri dishes and cultured cells on them. When i laser the cells i can see the laser and also the shape that is lasered, but it is not catapulted into the cap. So is there anyone who has the same problem or knows what is going wrong?
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Hi Manon! Any luck? Have you solved the issue?
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What are the factors of a telescope that influence the output power (in watts) of a laser beam passed through it, regardless of the laser's wavelength? Is there any relation in between laser power to telescope aperture ?
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The Conservation of Energy rule applies to a laser beam passing through a system or subsystem such as a telescope. Most of time, laser beam energy or power passing through a telescope will obey the following version of the conservation of energy rule:
P = T + R + A + S
Here “I” is the power input to the telescope and “T” is the power of the beam that is observed exiting the telescope. The losses are then “R” for reflection losses, “A” for absorption losses, and “S” is for scattering losses.
Reflection losses occur at each lens surface - these are specular reflections.
Scattering losses are just that, scattered light. Scattering losses occur in the bulk substrate and coatings of the lenses and are dependent on the wavelength of light and substrate/coating materials.
Scattering will also occur when a laser beam is too large for the telescope aperture. When this occurs a portion of the beam striking the telescope outside the lens clear aperture will be scattered.
Some of this light hitting the telescope body will also be absorbed in the housing.
Finally, absorption losses occur in even the best optics. Anytime a light or laser beam passes through an optic, a (hopefully 😊) small portion of the laser beam will be absorbed in the coatings and substrate materials of the lenses.
Second question: “Is there any relation in between laser power to telescope aperture?” For gaussian laser beams this is simple to answer using the power through an aperture equation. Wikipedia provides a good explanation of this at:
See specifically the section titled “Power through an aperture.” Of course, other types of laser beams require optical modeling in most cases.
This is by no means a comprehensive review, but I hope it helps a little!
You will also find some interesting information about lasers and optics at
If your interest is in gaussian beam stuff, click through to:
(you can log in as guest if you don't want to create an account - there is no charge in either case)
Gary
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Please let me know how to calculate the energy density for a continuous laser with wavelength of 940 nanometers, a power of 400 milliwatts, energy of 8 joules?
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Katayoun Salem any given beam area?
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Why Leakage current (Ices) becomes so high after laser annealing? Does anyone know the reason? Can it be due to generation of defects using Laser annealing compared to the conventional furnaces?
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IGBT levhalar ince bir yapıya sahip oldukları için katmanlar arasında herhangi bir kusur olduğu zaman levha zeminine gelen dalga kılavuzu hasar bırakır. Tavlama sırasında kimyasal olay meydana gelir. Dalga kılavuzu mikro dalgalar vb. dalga yayar. Tavlanmış zeminde kusur olması tavlama sırasında buzlanmaya neden olur. Buzlanmanın nedeni tavlama sırasında ışık ışınlarının malzeme kusurlarından dolayı ortamda bulunan gazlar vb. maddelerle tepkime vermesidir.
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How much is possible to concentrate a laser beam on a plane? For example is it possible to concentrate 4 laser beams on 4 close spots on a surface equal to 1 mm^2 without interference?
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A laser beam can be focused to an astonishingly small spot size. For example that is how they make the tiny features on your computer chips (at least down to the 50nm node anyway). The size of the focal spot depends on the wavelength and the cone angle. For a near diffraction limited beam you will get an Airy pattern. The radius from the center of the spot to the first Airy null is 1.22 x (wavelength) x NA where NA is numerical aperture and is the sine of the half cone angle.
Many lasers are nearly diffraction limited and can achieve something very close to the diffraction limited spot size. However others are not. Choices in how the beam is formed or aberrations in optics spoil the beam. This is called the beam quality. It is compared to the perfect diffraction limited beam by a parameter “M”. You would say that the beam is “M times diffraction limited”. Then the smallest spot size is M^2 the diffraction limit.
In your case the trick is the NA. How close together can you squeeze 4 lenses? And it’s not just the lens size, it’s the size of the beam going into the lens that determines NA. To avoid working with tiny lenses and short focal lengths you might want to expand the beam beam so it nearly fills a reasonable sized lens. The beams can be tilted towards each other to put the spots close together as you show, but that gets harder and harder as the NA gets larger.
So, for example, suppose we have 2 HeNe lasers (almost always very nearly diffraction limited) We expand the beams so the 1/e^2 diameter is 10 mm and put them into good aspheric lenses with diameter = 12.5 mm and focal length = 10 mm. That gives an NA of about 0.45 and a long enough focal length that the beams can be tilted together to put the spots next to each other as you show. In that case the Airy radius is about 1/3 of a micron. As I said, astonishingly small.
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We’ve got an LD laser matrix with 20 blue LDs (I attached the spec). We need to diffuse the beam to spread it over 20X20cm spot at 30-50mm. We need energy density variation <10% in the spot. I know how to do it by attaching fibers to emitters but it is not an option.
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Basically yes. If possible one should design and simulate the system.
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Hi everybody.
I would like to know if thre is any material whose fluorescent properties can be changed, in a permanent way, by a external stimulus, in particular by laser irradiation.
For example, let us say that a molecule exposed to UV light emits a blue fluorescence, but after being irradiated by a laser beam, when exposed to UV light its fluorescence shifts to red. It is just a example to explain the idea.
I do not mean laser induced fluorescence, but a permanent change in the fluorescent propoeties characteristcs of the molecule after being exposed to laser.
Thanks a lot in advance.
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Fluorescent photochroms have been studied in Japan. But I wonder if some are commercially available.
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How to Reasonably Weight the Uncertainty of Laser Tracker and the Mean Square Error of Level to Obtain Accurate H(Z)-value?
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Received, thank you for your answer。
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I have encountered an error to measure the light intensity of my laser source (650nm) (see image attached). The serial plot remains constant even i have changed the intensity of my light source, I have even tried both extremes: dark environment and close to lase source, yet there are no changes to the serial plot. Have anyone enconutered similar problem? How do i solve this error?
Here, the codes were used for the complete setup of photodiode BH1750 and Arduino Nano:
/*
Advanced BH1750 library usage example
This example has some comments about advanced usage features.
Connection:
VCC -> 3V3 or 5V
GND -> GND
SCL -> SCL (A5 on Arduino Uno, Leonardo, etc or 21 on Mega and Due, on esp8266 free selectable)
SDA -> SDA (A4 on Arduino Uno, Leonardo, etc or 20 on Mega and Due, on esp8266 free selectable)
ADD -> (not connected) or GND
ADD pin is used to set sensor I2C address. If it has voltage greater or equal to
0.7VCC voltage (e.g. you've connected it to VCC) the sensor address will be
0x5C. In other case (if ADD voltage less than 0.7 * VCC) the sensor address will
be 0x23 (by default).
*/
#include <Wire.h>
#include <BH1750.h>
/*
BH1750 can be physically configured to use two I2C addresses:
- 0x23 (most common) (if ADD pin had < 0.7VCC voltage)
- 0x5C (if ADD pin had > 0.7VCC voltage)
Library uses 0x23 address as default, but you can define any other address.
If you had troubles with default value - try to change it to 0x5C.
*/
BH1750 lightMeter(0x23);
void setup(){
Serial.begin(9600);
// Initialize the I2C bus (BH1750 library doesn't do this automatically)
Wire.begin();
// On esp8266 you can select SCL and SDA pins using Wire.begin(D4, D3);
/*
BH1750 has six different measurement modes. They are divided in two groups;
continuous and one-time measurements. In continuous mode, sensor continuously
measures lightness value. In one-time mode the sensor makes only one
measurement and then goes into Power Down mode.
Each mode, has three different precisions:
- Low Resolution Mode - (4 lx precision, 16ms measurement time)
- High Resolution Mode - (1 lx precision, 120ms measurement time)
- High Resolution Mode 2 - (0.5 lx precision, 120ms measurement time)
By default, the library uses Continuous High Resolution Mode, but you can
set any other mode, by passing it to BH1750.begin() or BH1750.configure()
functions.
[!] Remember, if you use One-Time mode, your sensor will go to Power Down
mode each time, when it completes a measurement and you've read it.
Full mode list:
BH1750_CONTINUOUS_LOW_RES_MODE
BH1750_CONTINUOUS_HIGH_RES_MODE (default)
BH1750_CONTINUOUS_HIGH_RES_MODE_2
BH1750_ONE_TIME_LOW_RES_MODE
BH1750_ONE_TIME_HIGH_RES_MODE
BH1750_ONE_TIME_HIGH_RES_MODE_2
*/
// begin returns a boolean that can be used to detect setup problems.
if (lightMeter.begin(BH1750::CONTINUOUS_HIGH_RES_MODE)) {
Serial.println(F("BH1750 Advanced begin"));
}
else {
Serial.println(F("Error initialising BH1750"));
}
}
void loop() {
float lux = lightMeter.readLightLevel();
Serial.print("Light: ");
Serial.print(lux);
Serial.println(" lx");
delay(1000);
}
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James Garry Hey mate, really appreciate your response to my question! It may sound really silly, apparently I have solved the issue by resoldering my microsensor. Now it works fine. Once again, thanks for the help mate!
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What is Laser Direct Infrared spectroscopy?
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You find a lot of explainations of the technology, if you use a search engine. Anything particular you don't understand?
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When two intense laser beams interact with a plasma, is second harmonic generation produced?
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Second harmonic generation (SHG) occurs when two laser beams beat in a plasma, producing harmonics. The process is more efficient when the beat frequency is the same as the resonant frequency. When the modulation frequency is twice that of the incident laser beams, the strength of the combined electric field is modulated. The efficiency and circumstances of SHG observation depend on experimental setup, laser properties, and plasma parameters.
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Can anyone tell me the equation to convert the laser beam intensity to db unit in origin software?
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dB is a logarithmic scale but laser beam intensity is measured by Photons per squared cm because it will be difficult. But you can normalize your laser beam intensity of signal by using some famous intensity such as output intensity of laser or unfocused laser beam intensity or by using output laserbeam intensity according to the specification of laser equipment.
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Generally unstable resonators have low M2 factor but still I do not know why and I have not a physical reasons behind this. We have some profile patterns in unstable resonator like doughnut shape in hard-edged resonators, it seems in this laser configuration the M2 factor is low.
In unstable resonators with VRM (Variable Reflectivity Mirrors) we have generally low M2 factor.
Basically I am looking for the main reasons or some examples to prove this fact.
If anyone has some information such a technical note, article or book, please let me know.
Best
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The higher the mode, the greater its losses, therefore only a certain number of the lowest modes are excited in the resonator. An unstable resonator has high losses for all modes, so only one or two “matching” the resonator geometry are excited in it. Which modes these will be, it depends on the specific geometry.
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I am working on an optical setup monitoring the power output of a 633nm 1.2mW laser. The light is polarised before entering a polarisation maintaining optical fibre in a thorlabs fiber launch clamp. The output is stable before the fibre, but very unstable after the fibre, so I know this is the source. Any suggestions on what might be causing this? Reducing the draft in the room and turning off the lights does not seem to be working. I am also using an optical bench with pneumatic isolators.
Any advice would be appreciated, thanks.
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Do you align the polarization of the laser to the axis of the fiber? It is absolutely necessary to align it to within a few degrees to avoid beating between the two output polarization modes. If your laser isn't polarized you'll need an extra polarizer.
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The laser 532 nm can penetrates around 700 nm depth in the sample, ( in the case, silicon cabide 4H) so then, the nickel silicide films we fabricated have around 150 nm thick. When we perform raman tests we can see only silicon carbide peaks. How to solve the depth problem of laser penetration ?
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Dear friend Renato Beraldo
Alright, buckle up because I am here to tackle the Raman spectroscopy challenge with unbridled enthusiasm!
Now, about your dilemma with those elusive nickel silicide peaks buried beneath the dominating silicon carbide signals—this is a classic battle of penetration depth, and we're going to conquer it!
1. **Adjust Laser Wavelength:**
- Try using a laser with a longer wavelength. Longer wavelengths generally penetrate deeper into materials. Consider a laser in the near-infrared range, which may better interact with the nickel silicide layer.
2. **Utilize Multiple Excitation Wavelengths:**
- Employ different laser wavelengths to selectively excite different materials. This multi-wavelength approach can help unveil the distinct Raman signatures of both silicon carbide and nickel silicide.
3. **Optimize Laser Power:**
- Adjust the laser power. Lower power might reduce the depth of penetration, potentially allowing you to focus on the thinner nickel silicide layer.
4. **Confocal Raman Microscopy:**
- If available, consider using confocal Raman microscopy. This technique uses a pinhole to eliminate out-of-focus light, enabling better depth resolution.
5. **Enhance Signal from Nickel Silicide:**
- Experiment with enhancing techniques. Surface-enhanced Raman spectroscopy (SERS) or resonance Raman scattering might amplify the nickel silicide signals.
6. **Thin Sectioning:**
- If feasible, consider thin sectioning your sample. Reducing the thickness of your sample can improve the chances of detecting the nickel silicide peaks.
7. **Sample Preparation:**
- Optimize sample preparation. Ensure a smooth and uniform surface. Any irregularities might affect the depth of focus.
8. **Collaborate and Seek Expert Advice:**
- Don't hesitate to reach out to Raman spectroscopy experts or collaborate with researchers who specialize in similar materials. Their experience could provide valuable insights.
Now, go forth, fearless experimenter Renato Beraldo! Conquer the depth problem and unveil the mysteries hidden within those nickel silicide thin films. I believe in you!
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If we want to laser metal or ceramic powder on a coating substrate, without having a powder injection source, how should we do this? That is, how to stick the powder on the substrate and then pass the laser over it?
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Cold spraying can be used to preset powders. By adjusting the process parameters of cold spraying, different layer thicknesses and uniformity levels of preset powder layers can be obtained
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I've had two articles published. Both are nearly identical, and I'd like to write a comparison article about their outcomes. For this, I'll need to use Comsol for simulation or machine learning/deep learning to validate the results. I'd appreciate it if someone could assist me in this area and contribute to the comparative essay.
  1. https://www.researchgate.net/publication/372887967_Formation_of_AgshellAucore_Bimetallic_Nanoparticles_by_Pulsed_Laser_Ablation_Method_Effect_of_ColloidalSolution_Concentration
  2. https://www.researchgate.net/publication/369671290_Optical_properties_of_synthesized_AuAg_Nanoparticles_using_532_nm_and_1064_nm_pulsed_laser_ablation_effect_of_solution_concentration
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This paper is in relevant with my goal:
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Specifically, I would like to determine the concentration of Marinobacter hydrocarbonoclasticus. Just like a UV-Vis, I will be using a GL55 photoresistor (as shown in the image attached) to calibrate the concentration of this bacteria by using a laser source. The choice I have available here is either 532nm (green) or 630nm (red) laser. I have found that 532nm is more likely to cause photobleaching to the photoresistor in the long run, since it has a higher photon energy than 630nm, however 532nm can be more sensitive to determine bacteria in a low concentration range. Hence, I would kindly like to seek advice from someone who has experience in developing a system involving LDR coupled with a laser. Thanks in advance!
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Photo-resistors are typically not used for quantitative measurements due to temperature drifts, etc. I would recommend to use photodiode instead, e.g. https://www.electronics-lab.com/project/photo-diode-amplifier-visible-light-using-opa381-arduino-nano-shield/
Either green or red should be ok with silicon photodiode.
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please i would like to collect all the parameters related to the synthesis of nanoparticles using laser ablation, in terms of laser parameters, liquid parameters, environment parameters and if any other parameters
Thanks
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The key parameters that can influence the size, shape, and properties of the nanoparticles produced are: Laser Parameters, Wavelength, Pulse Duration, Pulse Energy, Target Material, Type of Material, Liquid Environment, Type of Liquid and Experimental Setup and so on…
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I am using an Acousto optic deflector for scanning the laser beam. For producing a discrete light source, it is necessary to couple the 1st order diffracted beam from AOD into the single-mode fiber. However I could not couple the diffracted beam into the fiber core. Anyone, do you have any idea about that?
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1.dereceden kırınımlı ışık bir defa kırılsa bile çekirdek dışında kırılması gerekir. Çekirdek homojen olduğu için kırılmaya uğramadan geçmesi gerekir. Fiberden gelen enerjinin çekirdeğin enerjisine eşit olması gerekir.
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The longitudinal optical mode disappeared while the transverse optical mode increased with increasing laser intensity through Raman spectroscopy experiments. What happened in this case? Why?
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Hello Manuel,
My samlpe is a doped GaAs via chromium material.
Thanks
Javad
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Hello everyone,
In „LITT-Surgery“ ,how do we ensure, that we hit the target area (for instance a brain tumor)? And how do we actually control, that the brain tissue surrounding the target area stay intact, and not affected by the heat generated by the laser?
thanks in advance
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Amar Al Okla Usually the process is guided and controlled by MRI to ensure the location of tumor, positioning the fiber and monitoring the extent of ablation.
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Under what circumstances/application does one use Laser Vibrometer (which works under the principle of Doppler effect), Laser Triangulation Method and Laser Confocal Sensor. How does one determine which one is the best for a specifica application ? Also what is the difference when considering time to take one vibration measurement.
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Sorry Amiaan, Physics dictate that any sensor will measure the local level of vibration whatever the origin, so environment-induced vibration will equally be recorded by the LV. And you have to be further cautious to protect the LV device and mirrors from this environment-induced vibration...
As previously commented, if you use an accelerometer, its mass will also alter the vibration reading, which is not the case of LV - this is the main difference!
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The general characteristics of Satellite Laser Ranging (SLR): The photons returning are usually fewer because the transmitting laser and retroreflectors both have a divergence. This means that the laser beam spreads out as it travels, which can affect the accuracy of the measurement. How can this divergence be minimized?
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I suggest you read my article "Millimeter Accuracy Satellite Laser Ranging: A Review" available on Researchgate .
John Degnan
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Hello! Hope all is well with you. I am a freshman in the field of micro-nano optoelectronic device research. Recently, I was reading relevant literature about GaN-based lasers, and noticed that many literatures mentioned the concept of "unintentional doping" regarding factors affecting carrier transport. I tried to understand this concept through Google and other search engines. What I have learned so far is that compared to actively introducing impurities into intrinsic semiconductors, unintentional doping is doping caused by not actively introducing impurities. What are the factors that lead to the phenomenon of unintentional doping? Can unintentionally adulterated components be controlled artificially?
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It is a very tricky question. How can you control something unintentionally if unintentional means beyond control?
Unintentional doping is mostly caused by contamination. For example, when intentionally doping a wafer, metal traces on the wafer surface or on the doping source can also "unintentionally" diffuse. To "control" this, more precisely, to prevent this, high-purity materials and good cleaning procedures are needed.
Another example of unintentional doping is when you want to dope a specific area of the wafer, masks can be used to prevent unwanted diffusion.
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I have only ledit file how to make hard mask for mask aligner. is this possible to do using laser writer or engraver.
how to make pattern?
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Maske için gerekli bileşenler yeterli ise bundan sonra yapılacak en önemli şey kesim olur. Maskeyi istenilen boyutta kesmek için lazer ışın yöntemi ile yüzeye ışın kesme işlemi uygulanır.
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We are using Shimadzu SPM-9700HT AFM in the lab. It uses a 650nm laser for cantilever detection. I want to replace the laser to a longer wavelength to avoid excitation of fluorescent samples. If you have experience of replacing the laser unit, would you please be able to share the experience here?
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Normally laser in AFM system is focused on cantilever (only) and does no affect sample surface.
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PRIMES LDS software (PRIMES LASER DIAGNOSTIC SOFTWARE) is a well known tool for M2 measurements for laser beams. Anyone working with the software? Want to know the reason for taking multiple planes to measure M2. When we want to take a final result, which plane should be looked in to? If we are measuring a closer to a single mode beam, does each plane should give a Gaussian intensity distribution?
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Dear Chathurangani,
as Michael already pointed out, the measurement of M2 needs the profile in multiple planes of the beam caustic. As the product of beam waist and far field divergence (hence the name "beam parameter product"), both regions have to be measured, typically at at least 10 positions. The details can be found in the ISO standard 11146 and is implemented in the LDS.
Regarding the question of which plane to look at, it depends a bit on what your interested in. But yes, as already mentioned, a fundamental TEM00 (gaussian) mode would be shape-invariant (just scaling) along the propagation. By the way, pure higher TEM modes are also shape-invariant, although with a higher M2 value.
Best
Andreas
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Why does the laser for optogenetic stimulation need to be shuttered on and off rapidly? Can i use a constant laser light but at low power?
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I think Matthew and Elias are both answering the question, but from different perspectives.
Matthew is talking about activating neurones eg. with ChR2; then you’re looking at (relatively) fast pulsing on the order of 10 Hz. The goal here is to drive action potentials at the rate/pattern that they would fire naturally. You need to be careful not too pulse too quickly, as that also leads to inhibition in the same way Matthew explains for prolonged activation.
Elias is talking about limiting the light damage, which is particularly relevant when using inhibitory opsins. In this case, the ideal situation would likely be continuous light stimulation, but like Elias says that will lead to tissue warming and cellular damage. In theory, you could drop the light power to limit damage, but then you will drop below the activation threshold for the opsin and nothing will happen. Therefore, we do slow pulses (on the order of 0.1-1 Hz); for example, light on for 5 seconds, then off for 5 seconds. This compromise provides prolonged opsin activation while limiting tissue damage. Be careful not to do fast pulsing of inhibitory opsins, as you can induce reflex action potentials.
I have written about this exact issue on my blog, feel free to check it out and hopefully it will help answer the question in more detail:
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I am looking to purchase a corner cube for my laser resonator as a back mirror. Could you please advise on the necessary specifications to ensure I purchase the correct part? I have found that common market corner cubes, typically used for surveying land, are not durable enough for my needs.
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I agree with the others that in general it's a bad idea to use TIR from a glass or silica part to make a cavity mirror. It won't help with divergence in a properly designed cavity - if you unfold the rays it just looks like a flat mirror which will yield a very narrow stability range in a cavity with any kind of variable thermal lens. Here's one of the few examples I know of where this has been successfully utilized. Optical parametric oscillator with porro prism cavity EP0902911A1 European Patent Office.
If you have a short pulse laser then you have to start worrying about self-phase modulation in the glass causing beam filamentation. You'll see this as a fine line of very small bubbles in the bulk material where the beam is most intense. It won't necessarily be in the corner cube, depending on where the on where the dynamic intracavity waist is.
Another thing you have to worry about in addition to the polarization ambiguity, aberrations, and losses from where the beam touches the apices of the dihedrals, is that this is an intracavity element, so the circulating power is much higher than the output power. If you run the equations for the electric field strength on the internal TIR surfaces, you'll find that it is much higher than in the rest of the cavity, so it will be the first place you'll see catastrophic optical damage (COD), and before that photorefractive damage. You'll have to specify an exceptionally good, bubble and inclusion-free grade of glass, PH3 EVB for BK7 at a minimum or 3-axis-homogenous lithography-grade fused silica, in order to reduce the number of COD nucleation centers in the beam. The next thing you have to do is clean the outside TIR surfaces rigorously, and then house the part in a mount that keeps the dust off the places where the beam hits. If you don't, contact between the dust / dirt and the evanescent wave will be enough to cause catastrophic optical damage.
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How if a typical ED (Erbium-doped) fiber can be used as a CPA (coherent perfect absorber) for certain absorption Laser frequencies, e.g. 532nm, 650nm?
Or, if one can devise a ED fiber being the exact opposite of the laser process, i.e. make a design that we termed a CPA for certain application such as Raman Spectroscopy. My understanding is that the CPA can perfectly absorb incoming coherent laser light with given frequency and turns it into some form of internal energy — EM heat or energy.
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Over the last few months, just got learned more about the essence of possible implementation for the CPA fiber material, though it has not been proven yet.
Practically, while PM fibers are designed for maintaining polarization states, turning them into CPAs requires careful consideration of both the fiber's and the incoming light's properties. Given that the field of CPAs is still in its research stages, implementing it in PM fibers would require a deep understanding of both fields and might necessitate significant research and development.
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How the index profile of high power laser diode have changed from symmetric waveguide(WG) structure to the asymmetric WG? What is the advantage of the asymmetric waveguide structure?
optical loss?, COD threshold level?, resistance?, slope efficiency?, etc.
It would be helpful to get an entire overview of Historical Development of High power laser diode based on GaAs semiconductor.
the image reference : Overview of progress in super high efficiency diodes for pumping high energy lasers
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Simetrik dalga kılavuzunda p-tipi ve n-tipi malzemenin optik değişimi , eşik seviyesi, eğim verimliliği, direnc değişimi aynı şekilde artar veya azalır. Bu artış ve azalış birbirine göre simetriktir. Asimetrik olayda ise bu artma ve azalma n-tipi ve p-tipi malzemede farklı şekilde olur.
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ECDL is an external cavity diode laser, where we give a frequency dependent feedback to a laser diode to reduce its linewidth. Linewidths of the order of 10 MHz and less can be achieved by such a configuration. Can the same thing be done using an LED instead of a laser diode? Of course LED has a much larger linewidth than a laser diode, but can such a frequency selective feedback allow me to create such a laser? ( I thought of getting some expert opinions before attempting it in lab, because making an ECDL is a complicated process)
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Just some extra thoughts....
An ECDL works by using a frequency-selective element, such as a diffraction grating, to provide optical feedback to a laser diode, thereby narrowing its linewidth. This is possible because laser diodes are capable of producing coherent light, which means the light waves are in phase and have a definite relationship to each other.
LEDs, on the other hand, produce incoherent light. The light waves emitted by an LED are not in phase and do not have a definite relationship to each other. This is a fundamental difference between LEDs and laser diodes, and it is the reason why LEDs have a much broader linewidth than laser diodes.
Because of this fundamental difference, it is not possible to create an ECDL using an LED. The frequency-selective feedback mechanism of an ECDL relies on the coherence of the light produced by the laser diode. Since an LED does not produce coherent light, this mechanism cannot be used to narrow the linewidth of an LED.
In addition, LEDs typically operate in spontaneous emission mode, while laser diodes operate in stimulated emission mode. Stimulated emission is necessary for lasing to occur, and it is this process that allows the light waves to maintain phase coherence. LEDs, which operate in spontaneous emission mode, do not have this property.
In conclusion, while the idea of creating an ECDL with an LED is intriguing, it is not feasible due to the fundamental differences in the way LEDs and laser diodes produce light. The broad linewidth and incoherent light produced by LEDs make them unsuitable for the frequency-selective feedback mechanism used in an ECDL. However, successful use of ECDLs (with laser diodes) have been used extensively, as in the following paper in a temperature-measurement application:
.
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Hi, my intern and I were working on imaging V. corymbosum buds in a confocal microscope (LSM900). Our plan was to have the same settings for all the images, but we changed (by mistake) the gain and the laser intensity on each image. Is there a way to normalize all the images so they are comparable? We are targeting two different states of pectins (LM19 and LM20), and the plan was to see how their relative quantity changed across three sampling dates. Sadly we don't have time to stain the sections again and obtain new images, so we are searching for a way to use the already-acquired images.
Thank you!
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Yes, it is possible to normalize the images so that they are comparable despite the variation in gain and laser intensity settings. Normalization is a common technique used to remove variations caused by different acquisition settings and bring the images to a common scale for meaningful comparisons.
Here's a step-by-step guide on how to normalize your confocal microscope images:
1. Identify Reference Regions: Choose regions in the images that should remain constant across all the samples, ideally areas that are not affected by the gain and laser intensity changes. These regions will act as reference points for normalization.
2. Extract Pixel Value: Extract the pixel values of the selected reference regions from each image. This will create a dataset of reference values for all images.
3. Calculate Normalization Factors: For each image, calculate the normalization factors by comparing the reference values to the corresponding values in the other images. The normalization factor can be calculated as the ratio of the reference value in each image to the mean or median reference value across all images.
4. Apply Normalization: Divide each image by its corresponding normalization factor. This will effectively normalize the pixel intensities across all images, making them comparable.
Here's a simplified formula for the normalization process:
Normalized_image = (Original_image / Reference_value_of_image) * Mean_or_Median_of_Reference_values
Remember to perform the above steps separately for the LM19 and LM20 images if they are stained differently or require separate normalization.
After normalization, you can proceed with your original plan to compare the relative quantity of LM19 and LM20 pectins across the three sampling dates. Keep in mind that normalization helps to make the images comparable but does not correct for potential changes or degradation in the samples over time.
Always validate the results and carefully interpret the data, considering the limitations of the normalization process and other factors that may influence your analysis.
Camilo Villouta My recommendation to you is that if you will be planning an intensity profiling for your experiments you should always take the imaging at the same intensity.
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SLT is known to be effective after several weeks (4-6 weeks)
For advanced, newly diagnosed patients, is SLT an alternative for primary treatment, or are medications first better?
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Gençlerde ilaçla düzeltilemiyorsa slt'ye başvurulmalıdır. Fakat yaşlılarda slt'nin uygulanması tehlikelidir. Yaşlıların ilaçla devam etmesi daha uygun olur. Yaşlılarda göz hücreleri ve pigmentleri fazla canlı olmadığı için hiçbir yararı dokunmayabilir veya önceki görme yetilerini kaybedebilirler.
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God said, "Let there be light."
So, did God need to use many means when He created light? Physically we have to ask, "Should all processes of light generation obey the same equation?" "Is this equation the 'God equation'?"
Regarding the types of "light sources", we categorize them according to "how the light is emitted" (the way it is emitted):
Type 0 - naturally existing light. This philosophical assumption is important. It is important because it is impossible to determine whether it is more essential that all light is produced by matter, or that all light exists naturally and is transformed into matter. Moreover, naturally existing light can provide us with an absolute spacetime background (free light has a constant speed of light, independent of the motion of the light source and independent of the observer, which is equivalent to an absolute reference system).
Type I - Orbital Electron Transition[1]: usually determines the characteristic spectra of the elements in the periodic table, they are the "fingerprints" of the elements; if there is human intervention, coherent optical lasers can be generated. According to the assumptions of Bohr's orbital theory, the transitions are instantaneous, there is no process, and no time is required*. Therefore, it also cannot be described using specific differential equations, but only by probabilities. However, Schrödinger believed that the wave equation could give a reasonable explanation, and that the transition was no longer an instantaneous process, but a transitional one. The wave function transitions from one stable state to another, with a "superposition of states" in between [2].
Type II - Accelerated motion of charged particles emitting light. There are various scenarios here, and it should be emphasized that theoretically they can produce light of any wavelength, infinitely short to infinitely long, and they are all photons. 1) Blackbody radiation [3][4]: produced by the thermal motion of charged particles [5], is closely dependent on the temperature, and has a continuous spectrum in terms of statistical properties. This is the most ubiquitous class of light sources, ranging from stars like the Sun to the cosmic microwave background radiation [6], all of which have the same properties. 2) Radio: the most ubiquitous example of this is the electromagnetic waves radiated from antennas of devices such as wireless broadcasting, wireless communications, and radar. 3)Synchrotron radiation[7],e+e− → e+e−γ;the electromagnetic radiation emitted when charged particles travel in curved paths. 4)bremsstrahlung[8],for example, e+e− → qqg → 3 jets[11];electromagnetic radiation produced by the acceleration or especially the deceleration of a charged particle after passing through the electric and magnetic fields of a nucleus,continuous spectrum. 5)Cherenkov Radiation[9]:light produced by charged particles when they pass through an optically transparent medium at speeds greater than the speed of light in that medium.
Type III:Partical reactions、Nuclear reactions:Any physical reaction process that produces photon (boson**) output. 1)the Gamma Decay;2)Annihilation of particles and antiparticles when they meet[10]: this is a universal property of symmetric particles, the most typical physical reaction;3)Various concomitant light, such as during particle collisions;4)Transformational light output when light interacts with matter, such as Compton scattering[12].
Type IV: Various redshifts and violet shifts, changing the relative energies of light: gravitational redshift and violet shift, Doppler shift; cosmological redshift.
Type V: Virtual Photon[13][14]?
Our questions are:
Among these types of light-emitting modes, type II and type IV light-emitting obey Maxwell's equation, and the type I and type III light-emitting processes are not clearly explained.
We can not know the light-emitting process, but we can be sure that the result, the final output of photons, is the same. Can we be sure that it is a different process that produces the same photons?
Is the thing that is capable of producing light, itself light? Or at least contains elements of light, e.g., an electric field E, a magnetic field H. If there aren't any elements of light in it, then how was it created? By what means was one energy, momentum, converted into another energy hν, momentum h/λ?
There is a view that "Virtual particles are indeed real particles. Quantum theory predicts that every particle spends some time as a combination of other particles in all possible ways"[15]. What then are the actual things that can fulfill this interpretation? Can it only be energy-momentum?
We believe everything needs to be described by mathematical equations (not made-up operators). If the output of a system is the same, then the process that bridges the output should also be the same. That is, the output equations for light are the same, whether it is a transition, an accelerated moving charged particle, or an annihilation process, the difference is only in the input.
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* Schrödinger said:the theory was silent about the period s of transition or 'quantum jumps' (as one then began to call them). Since intermediary states had to remain disallowed, one could not but regard the transition as instantaneous; but on the other hand, the radiating of a coherent wave train of 3 or 4 feet length, as it can be observed in an interferometer, would use up just about the average interval between two transitions, leaving the atom no time to 'be' in those stationary states, the only ones of which the theory gave a description.
** We know the most about photons, but not so much about the nature of W, Z, and g. Their mass and confined existence is a problem. We hope to be able to discuss this in a follow-up issue.
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Links to related issues:
【1】"How does light know its speed and maintain that speed?”;
【2】"How do light and particles know that they are choosing the shortest path?”
【3】"light is always propagated with a definite velocity c which is independent of the state of motion of the emitting body.";
【4】“Are annihilation and pair production mutually inverse processes?”; https://www.researchgate.net/post/NO8_Are_annihilation_and_pair_production_mutually_inverse_processes;
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Reference:
[1] Bohr, N. (1913). "On the constitution of atoms and molecules." The London, Edinburgh, and Dublin Philosophical Magazine and Journal of Science 26(151): 1-25.
[2] Schrödinger, E. (1952). "Are there quantum jumps? Part I." The British Journal for the Philosophy of science 3.10 (1952): 109-123.
[3] Gearhart, C. A. (2002). "Planck, the Quantum, and the Historians." Physics in perspective 4(2): 170-215.
[4] Jain, P. and L. Sharma (1998). "The Physics of blackbody radiation: A review." Journal of Applied Science in Southern Africa 4: 80-101. 【GR@Pushpendra K. Jain】
[5] Arons, A. B. and M. Peppard (1965). "Einstein's Proposal of the Photon Concept—a Translation of the Annalen der Physik Paper of 1905." American Journal of Physics 33(5): 367-374.
[6] PROGRAM, P. "PLANCK PROGRAM."
[8] 韧致辐射;
[9] Neutrino detection by Cherenkov radiation:" Super-Kamiokande(超级神冈)." from https://www-sk.icrr.u-tokyo.ac.jp/en/sk/about/. 江门中微子实验 "The Jiangmen Underground Neutrino Observatory (JUNO)." from http://juno.ihep.cas.cn/.
[10] Li, B. A. and C. N. Yang (1989). "CY Chao, Pair creation and Pair Annihilation." International Journal of Modern Physics A 4(17): 4325-4335.
[11] Schmitz, W. (2019). Particles, Fields and Forces, Springer.
[12] Compton, A. H. (1923). "The Spectrum of Scattered X-Rays." Physical Review 22(5): 409-413.
[13] Manoukian, E. B. (2020). Transition Amplitudes and the Meaning of Virtual Particles. 100 Years of Fundamental Theoretical Physics in the Palm of Your Hand: Integrated Technical Treatment. E. B. Manoukian. Cham, Springer International Publishing: 169-175.
[14] Jaeger, G. (2021). "Exchange Forces in Particle Physics." Foundations of Physics 51(1): 13.
[15] Are virtual particles really constantly popping in and out of existence? Or are they merely a mathematical bookkeeping device for quantum mechanics? - Scientific American.
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There are few things wrong with this question.
One) light is a three dimensional phenomenon in nature that cannot describe by one dimension equation.
Two) science perception of light is unknown due to origination of light testing in past to now.
three) light in science is not natural light that we observe from suns, All light that we think is light, it is artificial light or flashlight, not the natural light that it does not have constant speed, because natural sunlight with massive frequencies and wavelength cannot have constant speed. Thus, science is wrong with light and sunlight.
Am i right? thanks.
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I am measuring PET-FCS of a peptide and after measuring for 90 min. I am noticing that there is no PET in the FCS figure. However, if I run the experiment for only 5 min, there is some PET. Also, the diffusion time decreases over time during the measurement. I am assuming that some kind of fragmentation is happening due to the excitation laser.
I will be glad if anyone can explain this fact or any possible theories are also welcomed.
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It seems your suggestion is right. Laser exposure even at red wavelength (620-670 nm) could induce permanent damage to peptides or other biomolecules, and rate of possible signal degradation should depend on laser power in focal volume and total volume of the sample.
There are 3 of possible effects: 1) peptide fragmentation, 2) permanent peptide conformation switching 3) peptide precipitation/condensation out from solution and seeing FCS signal from impurities in the solution.
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Dear colleagues….
why we use the laser off time to calculate the photothermal conversion efficiency not the on time?
Best Regards
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Because photothermal therapy is similar measurement of temperature. We are waiting till a heat of one body will be transferred to other. In our case we are waiting till the flashy light absorption will be distributed with sound speed in illuminated volume of our object to make results..
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I'm very much aware that the power output of CO2 laser can be varied by pulse width modulation (PWM). In my case I don't want to alter the laser pulse width but be able to vary the pulse energy. Is there a way around this? Thanks.
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That is simple question - use any type of attenuators and all be OK
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For Vacuum fluctuations based QRNG source, how will the linewidth of the laser source affects the shot noise. If we decrease the linewidth of the laser source, will we get a better output.
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Lazer kaynağının çizgi genişliği herhangi bir dalga boyunu tespit etmek için lazerin çizgi genişliğinde kalan kısmı lazer için periyot olarak alınır. Lazer çizgi genişliği ve vakum dalgalanması farklı bölgelere ve eşit periyotta alınır.