Questions related to Laser
Hi, I have a problem concerning my LIF measurements, intensity Vs concentration fails to be linear, also some dilutions have spectra lower than blank. Im working with a bacterial suspension about 10^8 cfu/ml. diluted from 10^-1 to 10^-10. also I tried another dilution sequence 1:10, 1:20, 1:40, 1:80, 1:160, 1:320, etc.... some dilutions have fluorescence intensity lower than blank which was normal saline. i need help, where am I going wrong.
I am working on laser moving heat source model in ansys thermal transient. I got the results like temprature variation of model over time. I need to measure melt pool depth by measuring the depth of isotherm of melting point of material from top and also the temperature gradient plot inside the melt pool. How can I get it?
On some samples I see this peak. It has nothing to do with the set up. I am using a 532nm laser. I think it could be correlated with the cleanliness of the surface. If the surface has a lot of particles on it, I get these peaks. This could be a false conclusion though. So far I have just found out that this peak could be due to Si-H stretching or sp1 C-C. Have you measured something similar? Is there an easy way to find out whether or not this is Si-H?
Thank you very much

We have a LIBRA (Coherent Inc) femtosecond laser amplifier (1 kHz). Recently, the oscillator of the laser (Vitesse, 80 MHz) is not getting mode-locked. We also have a Mai-Tai oscillator (80 MHz) with output power of 3 Watt. The LIBRA amplifier requires <170 mW of power for seeding. The pulse width of Vitesse output is <100fs and the Mai-Tai output pulse width is 80fs. Can I try to seed the LIBRA amplifier with the output of Mai-Tai? Kindly give your opinion based on your experience in a similar situation. Thanking you.
P K Datta
Dept. of Physics
IIT Kharagpur-721302
India
Im looking for a compact pulsed laser with 50-150 ns long pulses and prefereably 500-1000 mJ of pulse power. All Q-switched lasers I seem to find have 5-15 ns long pulses. (wavelength is not important). A MOPA fiber laser version can get me the pulse length right but not the power...
Any suggestions?
A lead-based perovskite single crystal was subjected to high-energy laser etching, and subsequent XPS spectral analysis of the etched region revealed the emergence of a loss feature peak (binding energy = 151 eV). Is the occurrence of this peak attributable to structural degradation of the crystal induced by laser damage? Does the intensity of the loss feature peak exhibit a correlation with the degree of structural deterioration? Could provide relevant references addressing this phenomenon?
I'm trying to find a solution that would measure the mode characteristics as accurately as possible and not be particularly expensive. Direct detection of high-power lasers can easily damage the device. I think an attenuator might be needed, but since the output light is linearly polarized, it is possible that the general attenuation method will change the pattern of the laser. Is there a better way to measure the nanosecond pulsed laser characteristics?
Dear Researcher
I am working with an Laser Doppler Vibrometer (LDV) to measure the natural frequencies and mode shapes of concrete bridges. While I have successfully applied LDV in the lab and on pedestrian cable bridges under controlled conditions, I now face a challenge when testing highway bridges.
🔹 The Challenge:
For certain bridge tests, it is not possible to position the vibrometer aligned with the vibration direction. As a result, the measured velocity component is along the laser beam direction, rather than the actual vibration velocity. This misalignment introduces errors in the frequency and mode shape estimations.
Key Questions for Discussion:
-Who has experience using LDV for bridge measurements, particularly in real-world conditions where perfect alignment is not possible?
-What methods have you used to correct the measured velocity and convert it into the true vibration velocity?
-Are there practical techniques, such as angular correction formulas or software adjustments, that you have successfully applied?
📌 Your Insights Matter!
If you have worked with LDV for bridge monitoring or have expertise in overcoming angular deviations, I would greatly appreciate your input. Sharing your experiences, methodologies, or references to relevant studies will be highly valuable.
Looking forward to an engaging discussion!

We need a photomask that can withstand high laser intensity. We use a 248nm excimer laser to perform photomask patterning processing on materials. The acting power on the photomask is 250MJ/cm², and the photomask needs to have long-term stability.
Dear forum users!
I have a question about the temperature in the Ideal gas equation when calculating the GHG flux, e.g. CO2 measured with LiCOR or Gas chromatography from the static chamber method.
I have found differences in the literature between them. So, my question is, what temperature should be used to calculate the flux?
Is it Air temperature, Inside chamber temperature, soil temperature, Laser temperature for LICOR/GC, or Laboratory ambient temperature for GC?
Thanks in advance.
Comparative outcomes of standard laser fistula closure (filac) versus flac combined with advancement flap in the treatment of complex anal fistula.
Could you please clarify whether suturing the internal opening was done in the standard FiLaC technique applied to the patients in your study?
The preliminary publication at Research Square states that the internal orifice was closed with a suture. However, nothing was mentioned in the paper published this month at Techniques in Coloproctology.
hello,
i've been using a spectra-physics Nd-YAG laser for the past 2 years working with the fundamental 1064 nm with each run about 2 hours. the power starts at 2.5 w and ends with about 1.3 watts. during the last week run, the laser spot died suddenly so i turned off the device and tried to run it again after 15 minutes. the laser spot starts well but it dies again after about 10 minutes. since then, the problem exists. can you please suggests any advice?
Ahmad
in many laser amp system,
Hi, I'm wondering how I can calculate the fluence of a laser from given parameters . like we have femtosecond laser with Bessel Beam .
1-160Mirowatt Power on power meter
2-10 Hz frequency
3-Beam spot diameter is unknown
4-50x with 0.6NA optical lens
According to the equation Fluence will calculate by F=E/A. But i am confused about Total power ,power on power meter ,pulse energy , laser spot diameter and the A in given equation. Is there any way to calculate the (fluence) of this laser from the mentioned parameters? what's the mathematical relation between given parameters
I would be truly grateful if anyone could answer me.
I have some cells marked with a plasmid producing luciferin/luciferase.
If I check the cells for their luminosity, I get very little signal. In fact people use specific camera that get images with very, very long exposures.
However, if I check the same cells with a confocal microscope, where the cells are painted with a monochromatic laser, then I can see a signal rather clearly.
What is the physics behind this kind of 'enhancement'?
Can it be that the laser is exciting luciferin producing a higher signal than luciferin alone would?
Thanks
- Hi! The SHG BBO crystal of our OPA system has detached from the holder, as shown in the photos attached. I want to adhere the crystal to the metal holder. I'm not very sure that whether I can use Norland UV curable optical adhesive NOA61? (I learned from the introduction of this adhesive that it can effectively stick glass optical components to metal surface.)
- I plan to apply glue to the four corners of the crystal and fix it to the holder, and then use UV light to cure it. Can you please tell me is it the right operation? What should I pay attention to?
- Thanks a lot for you support!

Upcoming Events "CONFERENCES" in Berlin, Germany |October 24-26 2025.
Global Meet & Expo on Astronomy, Astrophysics and Space Technology (GMEAAS2025):
I have been using 785 nm continuous laser for 20 months. 350 mW is the maximum output of the laser mentioned by the manufacturer. At the beginning I used to get the same power with my power meter. However, I can see the power is 285 mW, which means 20% dropped from the maximum power. Is there any suggestions why the power is dropping? any thumbs of rules that can predict the laser life time ?
A postgraduate student in pedodontics in India aimed to evaluate the diagnostic accuracy of the Diagnodent device for detecting early caries in school children. With a sample of 100 children aged 6 to 12 years, the student conducted a study at a local school. Each child underwent a clinical examination followed by a Diagnodent assessment, which uses laser fluorescence to identify carious lesions.
Suggest statistical solution for the above scenario &
Appraise how this study underscores the potential of integrating data science methods in clinical settings, paving the way for evidence-based dental practices?
I run an imaging core with a Zeiss Elyra 7 doing SIM. I have a new user doing some experiments using fixed, isolated cells. For some reason, he's getting tons of photobleaching with the 642 laser (not present in the other channels). He's using AF647 conjugated antibodies, only .5% laser power/100ms exposure. The only thing I can think of that might help this would be adding mounting medium to his dishes (right now he's just got an imaging buffer). [Also, JUST had my preventative maintenance visit, so I know it's not a problem with our 'scope]
Would this help stabilize his fluorophores? What's the best way to add mounting medium to a 35mm coverslip dish? Is that actually a thing?
Hello researchers,
I am constructing a PrismTIRF. I have a problem defining focus and detecting signals with labeled DNA (the bead sample works fine). I have looked for some possible solutions, yet have not been successful so far.
I wonder if the polarization type (p-polarized or s-polarized) of the laser can also cause this problem. Because I have read from some forums that a p-polarized laser can enhance the evanescent generation. My laser has a polarization ratio 100:1. Please share some experience with me.
Thank you.
Zero stands for emptiness, for nothing, and yet it is considered to be one of the greatest achievements of humankind. It took a long stretch of human history for it to be recognized and appreciated [1][4]. In the history of mathematics considerable confusion exists as to the origin of zero. There can be no unique answer to the query, "Who first discovered the zero?", for this may refer to any one of several related but distinct historical issues† [2]. A very explicit use of the concept of zero was made by Aristotle, who, speaking of motion in a vacuum, said "there is no ratio in which the void is exceeded by body, as there is no ratio of zero to a number” [3][2]*. He apparently recognized “the Special Status of Zero among the Natural Numbers.”
If we believe that zero is explicitly expressed mathematically, whether in number theory, algebra, or set theory, is the meaning of zero also clear and unified in the different branches of physics? Or can it have multiple meanings? Such as:
1)Annihilation——When positive and negative particles meet [5][6], e+e-=γ+γ',the two charges disappear, the two masses disappear, and only the energy does not disappear or increase; the momentum of the two electrons, which was 0, now becomes the positive and negative momentum of the two photons. How many kinds of zeros exist here, and what does each mean?
2)Double-slit interference—— The interference pattern in Young's double slit experiment, what exactly is expressed at the dark fringe? And how should it actually be understood? For light waves, it can be understood as the field canceling due to destructive interference and presenting itself as zero. For single photons, single electrons [7], physics considers it to be a probabilistic statistical property [12]. This means that in practice, at the dark fringes of theoretical calculations, the field will also be likely not to be zero‡.
3)Destructive interference——In Mach–Zehnder interferometer [8],there's always been a question of where the energy in the destructive interference arm went [9]? There seems to be an energy cancellation occurring.
4)Anti-reflection coatings——By coating [10], the reflected waves are completely canceled out to achieve the purpose of increasing transmission.
5)Nodes of Standing Waves——In optical resonant cavity, Laser Resonator. " The resonator cavity's path length determines the longitudinal resonator modes, or electric field distributions which cause a standing wave in the cavity "[13]. The amplitude of the electromagnetic field at the node of the standing wave is zero, but we cannot say that the energy and momentum at this point are zero, which would violate the uncertainty principle.
6)Laser Beam Mode——The simplest type of laser resonator modes are Hermite-Gaussian modes, also known as transverse electromagnetic modes (TEMnm), in which the electric field profile can be approximated by the product of a Gaussian function with a Hermite polynomial. TEMnm,where n is the number of nodes in x direction, m is the number of nodes in y direction [14].
7)Nodes of the Wave Function——Nodes and ends of the Wave Function Ψ in a square potential well have zero probability in quantum mechanics‡ [11]。
8)Pauli exclusion principle—— Fermions are antisymmetric,Ψ(q1,q2)=-Ψ(q1,q2), so Ψ(q1,q2)=0;Here a wave function of zero means that "field" is not allowed to exist, or according to the Copenhagen interpretation, the wave function has zero probability of appearing here?
9)Photon——zero mass, zero charge.
10)Absolute vacuum——Can it be defined as zero energy space?
11)Absolute temperature 0K——Is the entire physical world defined as a zero energy state except for photons?
12)Perfect superconductor—— "The three 'big zeros' of superconductivity (zero resistance, zero induction and zero entropy) have equal weight and grow from a single root: quantization of the angular momentum of paired electrons" [15].
13)......
Doesn't it violate mathematical principles if we may interpret the meaning of zeros in physics according to our needs? If we regard all zeros as energy not existing, or not allowed to exist here, does it mean that energy must have the same expression? Otherwise, we cannot find a unified explanation.
---------------------------------------------
Notes
* Ratio was a symmetrical expression particularly favored by the ancient Greeks.
† Symbols(0,...), words (zero, null, void, empty, none, ...), etc..
‡ Note in particular that probability itself is defined as a probability, not an exact value. For example, a probability of 0.5 can occur in physical reality as 0.49999999999, and it is almost never possible to have an accurate probability value such as 0.5. This means that there is no probability value that never occurs, even if the probability is theoretically 0. It is against the principle of probability to assume that a probability of zero means that it will never occur in reality.
---------------------------------------------
References
[1] Nieder, A. (2016). "Representing something out of nothing: The dawning of zero." Trends in Cognitive Sciences 20(11): 830-842.
[2] Boyer, C. B. (1944). "Zero: The symbol, the concept, the number." National Mathematics Magazine 18(8): 323-330.
[3] the Physics of Aristotle;
[4] Boyer, C. B. (1944). "Zero: The symbol, the concept, the number." National Mathematics Magazine 18(8): 323-330.
[5] https://www.researchgate.net/post/NO8Are_annihilation_and_pair_production_mutually_inverse_processes
[7] Davisson, C. and L. H. Germer (1927). "Diffraction of Electrons by a Crystal of Nickel." Physical Review 30(6): 705-740.
[8] Mach, L., L. Zehnder and C. Clark (2017). The Interferometers of Zehnder and Mach.
[9] Zetie, K., S. Adams and R. Tocknell (2000). "How does a Mach-Zehnder interferometer work?" Physics Education 35(1): 46.
[11] Chen, J. (2023). From Particle-in-a-Box Thought Experiment to a Complete Quantum Theory? -Version 22.
[12] Born, M. (1955). "Statistical Interpretation of Quantum Mechanics." Science 122(3172): 675-679.
[13]
[14] "Gaussian Beam Optics." from https://experimentationlab.berkeley.edu/sites/default/files/MOT/Gaussian-Beam-Optics.pdf.
[15] Kozhevnikov, V. (2021). "Meissner Effect: History of Development and Novel Aspects." Journal of Superconductivity and Novel Magnetism 34(8): 1979-2009.
Hello! The laser intensity in the source is displayed in amperes (on its screen), and my goal is to achieve a light irradiation of 100 mW/cm² given that the irradiated area is 0.125 cm², and the distance between the source irradiation and the irradiated surface (area) is 3 cm. So what current (in amperes) should I set my laser source on?
Thank you in advance.
How do we measure lifetime using continuous laser and PMT detector?
Let say I have a Mode-locked linear cavity fibre laser with 3m meter PM980 used for connect components within the cavity. Also, I am using a chirped fibre bragg grating (CFBG) for dispersion compensation.
PM980 has GVD of 0.014 ps^2/m
CFBG has D parameter = 0.42 ps/nm and reflection bandwidth of 9nm
laser pulse has FWHM width of 6nm
My first question is:
how to convert ps/nm of CFBG into ps^2/m?
is it simple as using β2=−2πc \ λ^2.D (since D is given in ps/nm, do I need to multiply it with pulse's bandwidth or CFBG bandwidth?)
Second question:
PM980 used within the cavity is 3m. Since in a linear cavity round-trip length is calculated as 2L, therefore, to calculate total group delay dispersion, one should multiply 2 x 3m ?
Thanks in advance!
I have two different populations: one expressing GFP and the other one expressing mcherry. I analyzed them individually (as well as a negative control) in the cytometer and I did compensation. However when I mix both populations a huge amount of events are positive for both GFP and mcherry. I thought maybe more than one bacteria were being exposed to the laser at one time so I diluted and lower the number of events per second. However the result seems to be similar. Conjugation when I mix both populations is discarded. Has anyone else had the same problem and could help me?
Thanks in advance
Following laser and source replacement of one of our Bruker Tensor 27 units, the calibration peak from the laser path through the empty chamber is registering a good amplitude, but it is far out of the normal range. I'm seeing a peak position at ~65000, when it should be between 58000 and 62000.
Is there any way to fix this? So far, tweaking the interferometer position only decreases the signal amplitude and does not alter the peak position coming in. The laser position in the holder seems fine. Any help would be appreciated.
Failed OQ and image of the display attached.. Thanks!

seti or search of intelgenece articial nee to be fixed due to masss solar systems outhere thta expelled laser beam light ollimated wel beign so is neede to modificated the seti in a new device of armenta velasquez like radi ho can detect the laser signals
When Er-doped materials are pumped by 980nm laser, they can emit the light at 1550nm as well as green light due to the energy transfer between different energy level. When all the emission energy come from the pump light, the up-conversion green light should be suppressed in order to maximize the emission energy at 1550nm which is the important for optical amplification at telecommunication wavelength. The question is thus that, how to suppress the up-conversion emission in Er-doped materials and therefore most of the pump energy can be converted into 1550nm?
Hi, I'd like to know if someone knows about a company where I can buy an affordable laser-scriber for perovskite films and another thin film for the fabrication of modules.
Many thanks,
i am unable to interpret why its increases in start as shown in figure

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

I heard that the intensity-dependence on the PL of the one-photon absorption is generally linear.
However, the intensity of PL at two-photon absorption increases to the quadratic power of the laser intensity. And three-photon does third power of the laser intensity.
Can you teach me a theoretical explanation?
Thank you.
I would like to use biofilmQ to analyse confocal laser image, how can do?
Sir,
I want to work on the laser assisted machining (turning) of Nickel Based Super alloy. Please help in finding the laser assisted machining facility in India.
Thanks and regards
I have a problem, in additive manufacturing (here specifically LPBF), if you make parts with different shapes through a fixed printing process, the parts will deform due to different geometry resulting in different stress concentrations. However, for the same shape of the part, the use of different printing process parameters to manufacture the part, will have an impact on its deformation? Such as different laser power, scanning rate, etc. (of course, if the part can be successfully manufactured)
Hello everyone, without going too much into details, my thesis is about improving the bonding strength at the wire bonding process. I should focus on laser pretreatment of the substrate. Has anyone some usefull knowledge/recommendations of literature etc?
I have a CVD-prepared material. The problem is that the LASER covers a smaller area than the islands, so I see localized effects. Sometimes, the intensity of the silicon peak is larger than the sample, and sometimes, it is otherwise. How do I deal with this?
I really appreciate any help you can provide.
I am working on a laser-based communication system for underserved areas, where a solar panel is used as a receiver and a laser as a transmitter. However, I am facing challenges in achieving the required signal strength due to limitations in laser capacity and solar panel conversion rates. I am exploring the possibility of modulating the laser signal onto sunlight to amplify the signal. Has anyone explored or achieved success in modulating laser signals onto sunlight? Are there any theoretical or practical limitations to this approach? Any insights or suggestions would be greatly appreciated.
Is it possible to detect microplastics in water through laser beam exposure?
I would be very thankful if somebody helps me with some practical advices about producing twin photons?
I know that twins are produced after a laser ray is directed on a BBO crystal. After the BBO there are two rings of photons and the twins are in the intersection of the rings.
1. I wonder in order to get the two rings must I have the laser ray falling strictly at 90 degree to the BBO? My crystal is very small 3x3 mm so I intend to focus the laser on it which means I would have different angles of them on the BBO so I fear I would not get the rings but a smeared spot. Is this right and what to do? I would like the beam to fall on a point but to be perpendicular. Is there a way to do this?
2. I am not at all sure how to capture the light from the intersection of the rings where the entangled twins are. I will filter the incident beam 405 nm by a filter at 405 but how to collect only the intersection spots to proceed and to direct them to a beam splitter b.e.? To make a mask with two openings? Or fibers?
Thanks in advance.
When using different laser energies, what is the effect on the size of nanoparticles? Does it increase or decrease with increasing energy? Please help
because I prepared magnetite by sol-gel method, I got XRD peaks, but for Raman, I didn't get a peak at the range 600cm-1, should I change the wavelength of the laser, or what do I have to do
I have a fluorescent probe that I'd like to test using flow cytometry, so that I can quantify its relative binding to different cell types. Unfortunately, the flow cytometer at my institution does not have a laser that reaches its recommended excitation wavelength. It does have a laser which overlaps its excitation spectrum, but only at a point which corresponds to 13% of the maximum excitation potential.
If I use this machine on samples treated with this probe is it possible that I can pick it up if the signal is strong enough, or am I risking inaccurate results?
The non-linear response of the electron in the driving laser field, we always mention in solids, inter-band polarization, and coherent intra-band dynamics of the electron. I want to deepen my understanding of the coherence related to the electron dynamics in the intra-band.
In fact, there is little evidence about this, but it seems that different types of laser light are produced by stars under different conditions.
Hello everyone,
I have already applied the Gaussian heat source and the next step is to apply recoil pressure in order to make the Keyhole.
I have read many papers but still not feeling confident to apply the recoil pressure. I would really appreciate If someone can explain the modules that I need to use for recoil pressure and some important settings in COMSOL.
Thank you.
Could anyone analyze about double passing of the laser beam through the febry perot etalon? Does it have negative effect on tuning the beam?
The reason I suspect that the beat note should not be broad linewidth (~100 MHz), as I see in the spectrum analyzer, is because, using those same lasers, we can create a magneto-optical trap. Therefore, the laser's linewidth should be less than 5 MHz (Cs D2 natural linewidth).
What is that I am doing wrong? What parameters should I check to mitigate this noise?
Thanks.
What factors or physical mechanism will affect the laser produced plasma (LPP) expansion process. How to reduce the instability of multiple measurements?
What is the relationship between the measured polarization rotation of far-detuned laser and the spin state of the atoms in Faraday effect?
A 532 nm laser probe with a 5 mm lens tube means, the laser can be focused at 5 mm distance from the lens tube. Similarly what I can infer from the -20 mm lens tube?
Thanks in advance.
When I was doing Raman spectroscopy, I observed that for the same sample (thin film), using two different laser sources gave different Raman spectra. We know Raman Shift is materially dependent property.What could be the reason for difference in Raman spectra?
Laser sources were the He-Cd laser (λ=325 nm), i.e., UV light source, and the He-Ne laser (λ=633 nm), i.e., visible light source.

Hi,
We need to buy a laser that is centered at 700-800 nm range (780 nm for example), has high output power (>100 mW), tunable (at least couple of nanometers), and has a linewidth preferably <1 MHz (not instantaneous linewidth). We got an offer from Toptica, but their delivery time is 32 weeks which will not work for us. If you know any other company that can deliver such a laser, we would be happy to hear.
Thanks so much
Would it than be possible to change the position of this beam systematically?
Would the position of the detection depend on the position of the beam left from the double slit plane?
Would the sum of all measurements result in the same known pattern of a double slit if all positions had been used for the same period of time?
I have added a file with an image to illustrate what I mean.
The answer could be very helpful in figuring out the path of photons near the slit. I have done some investigations, documented in
But the central question has not yet been answered: Is there a random effect in the double-slit experiment or not?
Hello,
I was measuring my sample - cellulose impregnated with polyethyleneimine, on a Raman microscope that has back-illuminated CCD. I used 633 nm excitation laser. In my spectrum, I got wavy fringes (due to interference?), but I don't know what causes them. I thought the etaloning effect was prominent only when using NIR laser, but I got the same results using 633 nm, 532 nm, and 455 nm laser (and not with 780 nm or 785 nm).

In literature I found different laser efficiency values starting from 30-45%, do you think it's due to the varying materials, metal powder quality, printing environment or the difference in efficiency is due to different brands/marks of metal 3D printers?
I want to know whether the temperature of the optical film must exceed the melting point of the material when it is ablated by laser.
I want to use the laser dissection function of the Zeiss PALM microscope. We have PEN membrane Petri dishes and cultured cells on them. When i laser the cells i can see the laser and also the shape that is lasered, but it is not catapulted into the cap. So is there anyone who has the same problem or knows what is going wrong?
What are the factors of a telescope that influence the output power (in watts) of a laser beam passed through it, regardless of the laser's wavelength? Is there any relation in between laser power to telescope aperture ?
Please let me know how to calculate the energy density for a continuous laser with wavelength of 940 nanometers, a power of 400 milliwatts, energy of 8 joules?
Why Leakage current (Ices) becomes so high after laser annealing? Does anyone know the reason? Can it be due to generation of defects using Laser annealing compared to the conventional furnaces?
How much is possible to concentrate a laser beam on a plane? For example is it possible to concentrate 4 laser beams on 4 close spots on a surface equal to 1 mm^2 without interference?

We’ve got an LD laser matrix with 20 blue LDs (I attached the spec). We need to diffuse the beam to spread it over 20X20cm spot at 30-50mm. We need energy density variation <10% in the spot. I know how to do it by attaching fibers to emitters but it is not an option.
Hi everybody.
I would like to know if thre is any material whose fluorescent properties can be changed, in a permanent way, by a external stimulus, in particular by laser irradiation.
For example, let us say that a molecule exposed to UV light emits a blue fluorescence, but after being irradiated by a laser beam, when exposed to UV light its fluorescence shifts to red. It is just a example to explain the idea.
I do not mean laser induced fluorescence, but a permanent change in the fluorescent propoeties characteristcs of the molecule after being exposed to laser.
Thanks a lot in advance.
How to Reasonably Weight the Uncertainty of Laser Tracker and the Mean Square Error of Level to Obtain Accurate H(Z)-value?
I have encountered an error to measure the light intensity of my laser source (650nm) (see image attached). The serial plot remains constant even i have changed the intensity of my light source, I have even tried both extremes: dark environment and close to lase source, yet there are no changes to the serial plot. Have anyone enconutered similar problem? How do i solve this error?
Here, the codes were used for the complete setup of photodiode BH1750 and Arduino Nano:
/*
Advanced BH1750 library usage example
This example has some comments about advanced usage features.
Connection:
VCC -> 3V3 or 5V
GND -> GND
SCL -> SCL (A5 on Arduino Uno, Leonardo, etc or 21 on Mega and Due, on esp8266 free selectable)
SDA -> SDA (A4 on Arduino Uno, Leonardo, etc or 20 on Mega and Due, on esp8266 free selectable)
ADD -> (not connected) or GND
ADD pin is used to set sensor I2C address. If it has voltage greater or equal to
0.7VCC voltage (e.g. you've connected it to VCC) the sensor address will be
0x5C. In other case (if ADD voltage less than 0.7 * VCC) the sensor address will
be 0x23 (by default).
*/
#include <Wire.h>
#include <BH1750.h>
/*
BH1750 can be physically configured to use two I2C addresses:
- 0x23 (most common) (if ADD pin had < 0.7VCC voltage)
- 0x5C (if ADD pin had > 0.7VCC voltage)
Library uses 0x23 address as default, but you can define any other address.
If you had troubles with default value - try to change it to 0x5C.
*/
BH1750 lightMeter(0x23);
void setup(){
Serial.begin(9600);
// Initialize the I2C bus (BH1750 library doesn't do this automatically)
Wire.begin();
// On esp8266 you can select SCL and SDA pins using Wire.begin(D4, D3);
/*
BH1750 has six different measurement modes. They are divided in two groups;
continuous and one-time measurements. In continuous mode, sensor continuously
measures lightness value. In one-time mode the sensor makes only one
measurement and then goes into Power Down mode.
Each mode, has three different precisions:
- Low Resolution Mode - (4 lx precision, 16ms measurement time)
- High Resolution Mode - (1 lx precision, 120ms measurement time)
- High Resolution Mode 2 - (0.5 lx precision, 120ms measurement time)
By default, the library uses Continuous High Resolution Mode, but you can
set any other mode, by passing it to BH1750.begin() or BH1750.configure()
functions.
[!] Remember, if you use One-Time mode, your sensor will go to Power Down
mode each time, when it completes a measurement and you've read it.
Full mode list:
BH1750_CONTINUOUS_LOW_RES_MODE
BH1750_CONTINUOUS_HIGH_RES_MODE (default)
BH1750_CONTINUOUS_HIGH_RES_MODE_2
BH1750_ONE_TIME_LOW_RES_MODE
BH1750_ONE_TIME_HIGH_RES_MODE
BH1750_ONE_TIME_HIGH_RES_MODE_2
*/
// begin returns a boolean that can be used to detect setup problems.
if (lightMeter.begin(BH1750::CONTINUOUS_HIGH_RES_MODE)) {
Serial.println(F("BH1750 Advanced begin"));
}
else {
Serial.println(F("Error initialising BH1750"));
}
}
void loop() {
float lux = lightMeter.readLightLevel();
Serial.print("Light: ");
Serial.print(lux);
Serial.println(" lx");
delay(1000);
}


When two intense laser beams interact with a plasma, is second harmonic generation produced?
Can anyone tell me the equation to convert the laser beam intensity to db unit in origin software?
Generally unstable resonators have low M2 factor but still I do not know why and I have not a physical reasons behind this. We have some profile patterns in unstable resonator like doughnut shape in hard-edged resonators, it seems in this laser configuration the M2 factor is low.
In unstable resonators with VRM (Variable Reflectivity Mirrors) we have generally low M2 factor.
Basically I am looking for the main reasons or some examples to prove this fact.
If anyone has some information such a technical note, article or book, please let me know.
Best
I am working on an optical setup monitoring the power output of a 633nm 1.2mW laser. The light is polarised before entering a polarisation maintaining optical fibre in a thorlabs fiber launch clamp. The output is stable before the fibre, but very unstable after the fibre, so I know this is the source. Any suggestions on what might be causing this? Reducing the draft in the room and turning off the lights does not seem to be working. I am also using an optical bench with pneumatic isolators.
Any advice would be appreciated, thanks.
The laser 532 nm can penetrates around 700 nm depth in the sample, ( in the case, silicon cabide 4H) so then, the nickel silicide films we fabricated have around 150 nm thick. When we perform raman tests we can see only silicon carbide peaks. How to solve the depth problem of laser penetration ?
If we want to laser metal or ceramic powder on a coating substrate, without having a powder injection source, how should we do this? That is, how to stick the powder on the substrate and then pass the laser over it?
I've had two articles published. Both are nearly identical, and I'd like to write a comparison article about their outcomes. For this, I'll need to use Comsol for simulation or machine learning/deep learning to validate the results. I'd appreciate it if someone could assist me in this area and contribute to the comparative essay.
- https://www.researchgate.net/publication/372887967_Formation_of_AgshellAucore_Bimetallic_Nanoparticles_by_Pulsed_Laser_Ablation_Method_Effect_of_ColloidalSolution_Concentration
- https://www.researchgate.net/publication/369671290_Optical_properties_of_synthesized_AuAg_Nanoparticles_using_532_nm_and_1064_nm_pulsed_laser_ablation_effect_of_solution_concentration
Specifically, I would like to determine the concentration of Marinobacter hydrocarbonoclasticus. Just like a UV-Vis, I will be using a GL55 photoresistor (as shown in the image attached) to calibrate the concentration of this bacteria by using a laser source. The choice I have available here is either 532nm (green) or 630nm (red) laser. I have found that 532nm is more likely to cause photobleaching to the photoresistor in the long run, since it has a higher photon energy than 630nm, however 532nm can be more sensitive to determine bacteria in a low concentration range. Hence, I would kindly like to seek advice from someone who has experience in developing a system involving LDR coupled with a laser. Thanks in advance!

please i would like to collect all the parameters related to the synthesis of nanoparticles using laser ablation, in terms of laser parameters, liquid parameters, environment parameters and if any other parameters
Thanks
I am using an Acousto optic deflector for scanning the laser beam. For producing a discrete light source, it is necessary to couple the 1st order diffracted beam from AOD into the single-mode fiber. However I could not couple the diffracted beam into the fiber core. Anyone, do you have any idea about that?
The longitudinal optical mode disappeared while the transverse optical mode increased with increasing laser intensity through Raman spectroscopy experiments. What happened in this case? Why?
Hello everyone,
In „LITT-Surgery“ ,how do we ensure, that we hit the target area (for instance a brain tumor)? And how do we actually control, that the brain tissue surrounding the target area stay intact, and not affected by the heat generated by the laser?
thanks in advance
Under what circumstances/application does one use Laser Vibrometer (which works under the principle of Doppler effect), Laser Triangulation Method and Laser Confocal Sensor. How does one determine which one is the best for a specifica application ? Also what is the difference when considering time to take one vibration measurement.
The general characteristics of Satellite Laser Ranging (SLR): The photons returning are usually fewer because the transmitting laser and retroreflectors both have a divergence. This means that the laser beam spreads out as it travels, which can affect the accuracy of the measurement. How can this divergence be minimized?
Hello! Hope all is well with you. I am a freshman in the field of micro-nano optoelectronic device research. Recently, I was reading relevant literature about GaN-based lasers, and noticed that many literatures mentioned the concept of "unintentional doping" regarding factors affecting carrier transport. I tried to understand this concept through Google and other search engines. What I have learned so far is that compared to actively introducing impurities into intrinsic semiconductors, unintentional doping is doping caused by not actively introducing impurities. What are the factors that lead to the phenomenon of unintentional doping? Can unintentionally adulterated components be controlled artificially?
I have only ledit file how to make hard mask for mask aligner. is this possible to do using laser writer or engraver.
how to make pattern?
We are using Shimadzu SPM-9700HT AFM in the lab. It uses a 650nm laser for cantilever detection. I want to replace the laser to a longer wavelength to avoid excitation of fluorescent samples. If you have experience of replacing the laser unit, would you please be able to share the experience here?
PRIMES LDS software (PRIMES LASER DIAGNOSTIC SOFTWARE) is a well known tool for M2 measurements for laser beams. Anyone working with the software? Want to know the reason for taking multiple planes to measure M2. When we want to take a final result, which plane should be looked in to? If we are measuring a closer to a single mode beam, does each plane should give a Gaussian intensity distribution?
Why does the laser for optogenetic stimulation need to be shuttered on and off rapidly? Can i use a constant laser light but at low power?
I am looking to purchase a corner cube for my laser resonator as a back mirror. Could you please advise on the necessary specifications to ensure I purchase the correct part? I have found that common market corner cubes, typically used for surveying land, are not durable enough for my needs.
How if a typical ED (Erbium-doped) fiber can be used as a CPA (coherent perfect absorber) for certain absorption Laser frequencies, e.g. 532nm, 650nm?
Or, if one can devise a ED fiber being the exact opposite of the laser process, i.e. make a design that we termed a CPA for certain application such as Raman Spectroscopy. My understanding is that the CPA can perfectly absorb incoming coherent laser light with given frequency and turns it into some form of internal energy — EM heat or energy.
How the index profile of high power laser diode have changed from symmetric waveguide(WG) structure to the asymmetric WG? What is the advantage of the asymmetric waveguide structure?
optical loss?, COD threshold level?, resistance?, slope efficiency?, etc.
It would be helpful to get an entire overview of Historical Development of High power laser diode based on GaAs semiconductor.
the image reference : Overview of progress in super high efficiency diodes for pumping high energy lasers

ECDL is an external cavity diode laser, where we give a frequency dependent feedback to a laser diode to reduce its linewidth. Linewidths of the order of 10 MHz and less can be achieved by such a configuration. Can the same thing be done using an LED instead of a laser diode? Of course LED has a much larger linewidth than a laser diode, but can such a frequency selective feedback allow me to create such a laser? ( I thought of getting some expert opinions before attempting it in lab, because making an ECDL is a complicated process)
Hi, my intern and I were working on imaging V. corymbosum buds in a confocal microscope (LSM900). Our plan was to have the same settings for all the images, but we changed (by mistake) the gain and the laser intensity on each image. Is there a way to normalize all the images so they are comparable? We are targeting two different states of pectins (LM19 and LM20), and the plan was to see how their relative quantity changed across three sampling dates. Sadly we don't have time to stain the sections again and obtain new images, so we are searching for a way to use the already-acquired images.
Thank you!
SLT is known to be effective after several weeks (4-6 weeks)
For advanced, newly diagnosed patients, is SLT an alternative for primary treatment, or are medications first better?
God said, "Let there be light."
So, did God need to use many means when He created light? Physically we have to ask, "Should all processes of light generation obey the same equation?" "Is this equation the 'God equation'?"
Regarding the types of "light sources", we categorize them according to "how the light is emitted" (the way it is emitted):
Type 0 - naturally existing light. This philosophical assumption is important. It is important because it is impossible to determine whether it is more essential that all light is produced by matter, or that all light exists naturally and is transformed into matter. Moreover, naturally existing light can provide us with an absolute spacetime background (free light has a constant speed of light, independent of the motion of the light source and independent of the observer, which is equivalent to an absolute reference system).
Type I - Orbital Electron Transition[1]: usually determines the characteristic spectra of the elements in the periodic table, they are the "fingerprints" of the elements; if there is human intervention, coherent optical lasers can be generated. According to the assumptions of Bohr's orbital theory, the transitions are instantaneous, there is no process, and no time is required*. Therefore, it also cannot be described using specific differential equations, but only by probabilities. However, Schrödinger believed that the wave equation could give a reasonable explanation, and that the transition was no longer an instantaneous process, but a transitional one. The wave function transitions from one stable state to another, with a "superposition of states" in between [2].
Type II - Accelerated motion of charged particles emitting light. There are various scenarios here, and it should be emphasized that theoretically they can produce light of any wavelength, infinitely short to infinitely long, and they are all photons. 1) Blackbody radiation [3][4]: produced by the thermal motion of charged particles [5], is closely dependent on the temperature, and has a continuous spectrum in terms of statistical properties. This is the most ubiquitous class of light sources, ranging from stars like the Sun to the cosmic microwave background radiation [6], all of which have the same properties. 2) Radio: the most ubiquitous example of this is the electromagnetic waves radiated from antennas of devices such as wireless broadcasting, wireless communications, and radar. 3)Synchrotron radiation[7],e+e− → e+e−γ;the electromagnetic radiation emitted when charged particles travel in curved paths. 4)bremsstrahlung[8],for example, e+e− → qqg → 3 jets[11];electromagnetic radiation produced by the acceleration or especially the deceleration of a charged particle after passing through the electric and magnetic fields of a nucleus,continuous spectrum. 5)Cherenkov Radiation[9]:light produced by charged particles when they pass through an optically transparent medium at speeds greater than the speed of light in that medium.
Type III:Partical reactions、Nuclear reactions:Any physical reaction process that produces photon (boson**) output. 1)the Gamma Decay;2)Annihilation of particles and antiparticles when they meet[10]: this is a universal property of symmetric particles, the most typical physical reaction;3)Various concomitant light, such as during particle collisions;4)Transformational light output when light interacts with matter, such as Compton scattering[12].
Type IV: Various redshifts and violet shifts, changing the relative energies of light: gravitational redshift and violet shift, Doppler shift; cosmological redshift.
Type V: Virtual Photon[13][14]?
Our questions are:
Among these types of light-emitting modes, type II and type IV light-emitting obey Maxwell's equation, and the type I and type III light-emitting processes are not clearly explained.
We can not know the light-emitting process, but we can be sure that the result, the final output of photons, is the same. Can we be sure that it is a different process that produces the same photons?
Is the thing that is capable of producing light, itself light? Or at least contains elements of light, e.g., an electric field E, a magnetic field H. If there aren't any elements of light in it, then how was it created? By what means was one energy, momentum, converted into another energy hν, momentum h/λ?
There is a view that "Virtual particles are indeed real particles. Quantum theory predicts that every particle spends some time as a combination of other particles in all possible ways"[15]. What then are the actual things that can fulfill this interpretation? Can it only be energy-momentum?
We believe everything needs to be described by mathematical equations (not made-up operators). If the output of a system is the same, then the process that bridges the output should also be the same. That is, the output equations for light are the same, whether it is a transition, an accelerated moving charged particle, or an annihilation process, the difference is only in the input.
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* Schrödinger said:the theory was silent about the period s of transition or 'quantum jumps' (as one then began to call them). Since intermediary states had to remain disallowed, one could not but regard the transition as instantaneous; but on the other hand, the radiating of a coherent wave train of 3 or 4 feet length, as it can be observed in an interferometer, would use up just about the average interval between two transitions, leaving the atom no time to 'be' in those stationary states, the only ones of which the theory gave a description.
** We know the most about photons, but not so much about the nature of W, Z, and g. Their mass and confined existence is a problem. We hope to be able to discuss this in a follow-up issue.
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Links to related issues:
【1】"How does light know its speed and maintain that speed?”;
【2】"How do light and particles know that they are choosing the shortest path?”
【3】"light is always propagated with a definite velocity c which is independent of the state of motion of the emitting body.";
【4】“Are annihilation and pair production mutually inverse processes?”; https://www.researchgate.net/post/NO8_Are_annihilation_and_pair_production_mutually_inverse_processes;
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Reference:
[1] Bohr, N. (1913). "On the constitution of atoms and molecules." The London, Edinburgh, and Dublin Philosophical Magazine and Journal of Science 26(151): 1-25.
[2] Schrödinger, E. (1952). "Are there quantum jumps? Part I." The British Journal for the Philosophy of science 3.10 (1952): 109-123.
[3] Gearhart, C. A. (2002). "Planck, the Quantum, and the Historians." Physics in perspective 4(2): 170-215.
[4] Jain, P. and L. Sharma (1998). "The Physics of blackbody radiation: A review." Journal of Applied Science in Southern Africa 4: 80-101. 【GR@Pushpendra K. Jain】
[5] Arons, A. B. and M. Peppard (1965). "Einstein's Proposal of the Photon Concept—a Translation of the Annalen der Physik Paper of 1905." American Journal of Physics 33(5): 367-374.
[6] PROGRAM, P. "PLANCK PROGRAM."
[8] 韧致辐射;
[9] Neutrino detection by Cherenkov radiation:" Super-Kamiokande(超级神冈)." from https://www-sk.icrr.u-tokyo.ac.jp/en/sk/about/. 江门中微子实验 "The Jiangmen Underground Neutrino Observatory (JUNO)." from http://juno.ihep.cas.cn/.
[10] Li, B. A. and C. N. Yang (1989). "CY Chao, Pair creation and Pair Annihilation." International Journal of Modern Physics A 4(17): 4325-4335.
[11] Schmitz, W. (2019). Particles, Fields and Forces, Springer.
[12] Compton, A. H. (1923). "The Spectrum of Scattered X-Rays." Physical Review 22(5): 409-413.
[13] Manoukian, E. B. (2020). Transition Amplitudes and the Meaning of Virtual Particles. 100 Years of Fundamental Theoretical Physics in the Palm of Your Hand: Integrated Technical Treatment. E. B. Manoukian. Cham, Springer International Publishing: 169-175.
[14] Jaeger, G. (2021). "Exchange Forces in Particle Physics." Foundations of Physics 51(1): 13.
[15] Are virtual particles really constantly popping in and out of existence? Or are they merely a mathematical bookkeeping device for quantum mechanics? - Scientific American.
I am measuring PET-FCS of a peptide and after measuring for 90 min. I am noticing that there is no PET in the FCS figure. However, if I run the experiment for only 5 min, there is some PET. Also, the diffusion time decreases over time during the measurement. I am assuming that some kind of fragmentation is happening due to the excitation laser.
I will be glad if anyone can explain this fact or any possible theories are also welcomed.
Dear colleagues….
why we use the laser off time to calculate the photothermal conversion efficiency not the on time?
Best Regards
I'm very much aware that the power output of CO2 laser can be varied by pulse width modulation (PWM). In my case I don't want to alter the laser pulse width but be able to vary the pulse energy. Is there a way around this? Thanks.
For Vacuum fluctuations based QRNG source, how will the linewidth of the laser source affects the shot noise. If we decrease the linewidth of the laser source, will we get a better output.