Laboratory Weighing - Science method

We have purchased 1 mg of a neat polyethylene standard and would like to weigh out 250 mg of it to use as a standard for our microplastics research. We are sending out our samples to a contractor for pyr-GC/MS analysis for mass and polymer identification. We want to send them a known mass of the PE. The PE adheres to the bottle -static charge? We tried using a static gun but that doesn't seem to work. What is the best way to measure out the PE accurately?

Please help me

Hello,

I would like to calculate the doping percentage. I want to do Mg doped CuO nanoparticles (5% & 7%). Can anyone help me to calculate the material i need to weigh? Also do i have to make two different solutions of this metals or adding one metal in another metal solution?

Regards.

we have to make 10ml solution.

how to measure 0.2mg while our lab doesn't have weighing machine to measure 0.2mg. so do we have to make a stock solution and then have to dilute? please explain.

I want to dissolve caseinFITC (commercial) in ddH2O and store it at -80 to have standard aliquotes (instead of weighing powder each time which is prone to mistakes). Literature says unconjugated FITC is not stable in water and should not be stored. They suggest dissolving in DMSO or acetone to stoe longer but this is not possible due to my experimental setting. I am wondering if the stabilty of FITC is effected by casein conjugation. Can I store caseinFITC dissolved in water and would it be stable on the contrary of unconjugated FITC?

Salts usually remain unmixed in the solutions, even after vigorous mixing, while preparing buffers for DNA isolation. Is there a method to it?

Hi everyone, I came to you because I know there is people here with novel knowledge in the AI realm, so it would be awesome if you take some time to weigh on the following question! What's the current best T2V model that is performative and capable of generating long and high-resolution videos? If there isn't one yet, what are the latest ongoing studies that are close to yield the way for such an innovation?

We want to study the growth and development of plant roots under different soil moisture contents. Considering the advantages and disadvantages of several commonly used water control methods, we would like to seek your help to explore a relatively simple and accurate water control method for potted seedlings.

1. Weighing method. A commonly used method is to determine the amount of lost water through weighing and replenish it in a timely manner. But once there are too many biological replicates in our treatment group, it is time-consuming and labor-intensive, and we spend a long time in the weighing and hydration process. On the other hand, as plants grow, their own weight also changes, making the addition of water to each weighing method unscientific.

2. Moisture monitoring instrument. According to the moisture monitoring instrument, the soil moisture content was detected, but according to the several moisture detection instruments we purchased, it was found that there were accuracy issues. At the same time, the measurement value largely depends on the placement of the sensor probe and the influence of the watering position

3. PEG simulation. We would like to know how long PEG can be maintained, i.e. the validity period during treatment. We may consider combining short-term and long-term water control to determine if long-term testing is feasible.

4. After watering, control the water naturally. It can be used for short-term water control, stopping water supply after initial watering, and observing the plant's response during gradual water loss. But we believe that in this situation, there may not be significant differences in the root system, and a long-term water control situation is needed to make the root system different from the control group.

We sincerely invite everyone to help provide a scientifically feasible method for controlling water in potted seedlings. We are extremely grateful for this.

Hi, I should prapare a standard curve of IAA and I don't know the method is. For measuring of IAA in bacteria, I culture bacteria in TSB medium and mix the supernatant of inoculated medium with salkowski solution (1: 2). Should I mix the IAA with salkowski in standard curve? And how much IAA should I weigh and solve in NAOH?

Thanks

methylene blue

stock solution

I have been boggled by a seemingly simple problem. Many studies state that "The water contents of the pots were checked daily by weighing and adjusted to field capacity."

How is this performed? What pre-tests should I carry out to know approximately how much water I should be adding to my pots to keep them at FC? Anyone?

I have 0.2ml of essential oil X weighing about 200mg, I wanted to diluted it to a final volume of 5 ml, calculate its concentration in mg/ml ?

Thank you.

Q 1. Which model should be preferred (FE/RE) when conducting a meta-analysis for pooling prevalence?

Q2. In the RE model, why do all studies receive equal weight irrespective of sample size or confidence intervals?

Q3. When we use the FE model, all the studies receive weight according to their sample size or CI. But my question is, is it correct to use the Fixed Effect Model?

Does herbicidal residues used during termination of cover crops have impact on soil health? Other alternatives for cover crops termination may have benefits over use of herbicidal residues.

Hello fellow researchers and innovators

At the moment I am working for a small / medium Norwegian municipality. There we want to develop a method, which enables us to make climate positive decisions when planning, building and maintaining urban public spaces w/ and w/o greenery.

This municipality is suffering rural migration, which means, the economics isn't the best. So we need to find / work out our own solutions instead of buying.

In addition to that, most of the software and calculators I have come across are developed for "buildings", hard materials for housing constructions, but in urban public places w/ greenery vegetation can account for CO2-sinks.

I feel the benefits of plants are far too often forgotten.

Do any of you have possible "links" to interesting webpages, or perhaps and even better, do any of you have an Excel-sheet which one could use to calculate this?

You know, perhaps someone has already done this job and worked out how to give factors and benefits and measurements into a calculation matrix, which then could work out a weighing up of a variety of design-elements...?

Your name would of course be mentioned and the research / product would be given your credits / copyright...

Cheers,

Berit

I am planning to perform C13 MFA, and I was wondering how I can weigh the labeled glucose and add it to the media while maintaining sterility. I didn't find any protocol specifically explain this part.

I am releasing curcumin in a PBS/ethanol solution. The concentration of my drug is 5mg/ml in the encapsulation and I weighed 20mg of the loaded sample to perform the release studies. I take absorbance of the data at specific time intervals, after which I replace the same volume of PBS withdrawn. Volume withdrawn is 3ml out of a 10ml PBS/ethanol solution.

Forest litter was sampled from 1 x 1 m plots, weighed as W1 and a sub-sample was taken. The subsample was also considered as (W2) before being taken to the laboratory for oven drying. After the oven-dried, the subsample was weighed as the final weight (W3).

From the above, please how can I estimate the biomass?

I'm designing a new activity for an undergraduate biology course about enzyme activity. I'm running into a slight complication. I've ordered a lyophilized powdered version of human salivary alpha amylase.

The issue is "how long can it be stored once dissolved?".

Most protocols say to use "freshly made" enzyme. But the amount needed for a lab section is too small to weigh out. Seriously, the bottle has 1000 Units and is only 10 milligrams. Diluting to 1 Unit/mL for a working concentration makes 1 Liter of enzyme solution. That is more than enough for an entire week of lab sections!

But, will it denature/lose too much activity in the refrigerator?

Looking for some practical advice!

As essential oils are in liquid form, they are usually weighed in milliliters. However, in various studies, it is converted to powder and measured in milligrams by following different methods that can result in the loss of its vital activities.

Speed=Utter Energy*Velocity Equation…. Speed=F*dx,Then Speed=ma*dx,Then Speed=m*v/t*dx,Then Speed=mdx/t*v,Then Speed=Utter Energy*Velocity.S=UV..Simple Equation but my Very Favorite…And I Guess This Equation Speed=Utter Energy*Velocity Equation It Goes Everyside In Science In Universe….People-Animal-Fish Walk-Run-Planet Movement-Chemistry Atom Velocity And Everyside In Science In Universe It Goes.Here At Elements Mass And Utter Energy Comes With Mass Inside Atom Velocity When Atom Distribution And Distance comes With Time And Utter Energy Velocity Comes With Mass And Atom Distribution. By Utter Energy And By Environmental Partial Reaction And Environmental Reaction Another Velocity Comes Which Velocity Increase And Decrease Depend On Environmental Partial Reaction With Environmental Attraction And Repulsion And Increase And Decrease.Utter Energy And Mass Comes From Elements Mass And Mass Inside Atom Velocity With Distance And Time And By Environmental Partial Reaction And Environmental Reaction Another Velocity Comes Which Velocity Increase And Decrease Depend On Environmental Partial Reaction With Environmental Attraction And Repulsion And Speed Goes To=Utter Energy*Velocity And Work-Energy And Kinetic Energy Equation Gonna E=mv2Square...Man,Animal,Bird,Fish Walk- Run And Others All Equation Same And It Is Speed=Utter Energy* Velocity.And I Guess This Equation Speed=Utter Energy*Velocity Equation Is Number One Equation Is Universe Among Trillion Trillion Equation In Universe Cause It Goes Everyside In Universe….People-Animal-Fish Walk-Run-Planet Movement-Chemistry Atom Velocity And Everyside In Science In Universe It Goes

Work-Energy And Kinetic Energy Equation……Physics Books Says…..E=1/2MV2(SQUARE)……And Work=1/2MV2(SQUARE)……….But Here Acceleration And Velocity Uniform But Nothing Is Uniform In Universe…….But My Equation….

And Simply…..Kinetic Energy Or Work Or Energy Equation=F*Dx….. Then..W=ma*dx...W=m*v/t*dx….W=mvdx/t…..W=mv2(SQUARE)……….Here Dx=Distance,T=Time,V=Velocity,a=Acceleration

Work Equation= We Know W=F*Dx...Then..W=ma*dx...W=m*v/t*dx...Now....W=M*V*Dx/t..W=M* Δm (Delta Mass)/Δt (Delta Time)*dx/t...Now..W=M*V* Δm (Delta Mass)/Δt

Now...W=M*V* Δm/Δt…….W=MV Δm/ Δm/V……..W=mv2(SQUARE)……….

HERE, Δm (Delta Mass)= V× Δt……And…. Δt= Δm/V

I Guess my equation is correct cause earth physics books Says…. Kinetic Energy Equation-Energy Equation And Work Equation……Physics Books Says…..E=1/2MV2(SQUARE)……And Work=1/2MV2(SQUARE)……….But Here Acceleration And Velocity Is Uniform…..but nothing is uniform In Universe…At Our Physics Book Work-Energy-Kinetic Energy Equation=1/2 mv2square But Here Velocity And Acceleration Uniform But Nothing Is Uniform In This Universe...Atom Velocity,Air Velocity,Water Velocity,Light Velocity And Nothing Is All Time Uniform In This Universe But Our Physics Books Many Many Equations And Isac Newton Equations Depends On Uniform Velocity And Acceleration Equations.but nothing is uniform In Universe

.

*********I Have Physics Four Force Accommodation Rule.When Gravitational Force Strong Then Weak Nuclear Force Strong Then Electro-Magnetic Force Strong Then Strong Nuclear Force Strong.When Weak Then All Are Weak. Now If Gravitational Strength=Mgh/tan90.I said One cm degree angle if increase then it will be 1.135cm(According to my Rule). Now Gravitational Force Strength=Mgh/tan90 that 1.135*90. Now Weak Nuclear Force Strength=M*(-NB that break nucleoli beta)gh/tan 90 that 1.135*90. Now Electromagnetic Strength=M*EES(Electromagnetic Energy)gh/tan90 that 1.135*90. Now Strong Nuclear Force Strength=M*(+NB that Compact Nucleoli Beta)gh/tan90 that 1.135*90. Now If we will identify Four Force math then it will be When Gravitational Force Strong then Others Three Force Strong,When weak all Are weak. .Now Simply.it will be just 4force Accommodation=mgh{1+{-NB(Less or huge)+EME that electromagnetic energy+(+NB Less or Huge}}/tan90 that 1.135*90

*******Desert Mirage Equation=Light Intensity*Warm Vapor.When Light Intensity high then Silica burns Inside and warm Vapor Comes.Why it is not seen from near but seen from far cause vapor ripple.When vapor comes then its ripple small.When we see from far then we can see large length ripple cause ripple become slowly large.When we see from far then we see large ripple&we can see mirage.when we see from near then ripple length small&we cannot see any mirage.This is the Desert Mirage Mystery And Also Cause For Road Mirage Mystery….We Can Also Prove It By Water Hot And Make Vapour

Jupiter,Mars,Venus,Moon Planet Mystery……….. Other Planets People Who Are Live Inside{Under Soil Surface} Those Planets Like Jupiter,Mars,Venus,Saturn And Others Cause They Are Rolling On Magnetic Force And Light Is Absorbing There. And It’s Also True Creator Also Showed Me Symptom That Signs And Creator Also Confirms Me That There Are People Live Inside That Under Soil Surface Others Planet Around The Earth Like Jupiter,Mars,Venus,Neptue,Saturn,Urenus And Others Cause They Are Rolling On Magnetic Force And Light Is Absorbing There. Those Planets Just Like Coconut Fruit That Upper Surface Normal After That Hard Surface Inside And After That Blank Inside .And If We Will Digging Right Upper Corner Then We Will Be Able To Enter Inside.Cause Only Right Upper Corner Side Pale And Others Everyside Thick. At-First It Should Be Come In Mind It Is Quiet Impossible Or Unbelievable That People Live Inside Others Planet.But Truth Is Actualy They Are Live Inside.And There Radio Frequency Will Not Work Cause Radio Frequency Weak Frequency And It Is Not Go So Under Of Soil.You Can Use Light Or Satellite Or Sound Frequency Or My Light Absorb Equation Frequency. Then You Must See Them.Why They Couldnot Or Cannot Come Outside Cause May Be They Did not Or Donot Know How They Can Come Outside.Otherwise Not Every Planet But Many Planets Can See Sun,Moon,Twinkle Star For Light Frequency When They Are Live Inside But Every Planet Cannot See.Now We Have Electric Recycling And Triangle Vehicle And Missile Like Electric Force.We Can Go Any Planet This Sky Or Invisible World Sky….

About Gog And Magog (Bible) That Yajuj And Majuj (Quran)………… I Told You About Gog And Magog(Bibel) That Yajuj And Majuj(Quran) Who Are Live Inside This Earth And They Will Be Come And Attack You Just Like Ripple Wind Ripple Within Very Short Time And Many People Will Be Die Or Can Be Die.And I Told You Some Places Name In Earth If You Have Frequency Which Go Under 500 Kilometer Of Soil {Under Soil Surface}Than You Must Be See Them Those Places And Creator Allah Showed Me Symptom That Signs Those Places Like Very Vast Near Iran And Turkeministan Border{Like "Mashahad" Name Place}.You Can Go And Fall Frequency On Turkeministan Near Iran Border Mashahad Name Place Or Turkeministan To Near Iran Border And Take Prove Yourself Of My Word That I Am Cheater Or True. And You Must See They Are Also Vast Caste{Tribal} Like You.I Told You Some Places Name In Earth If You Have Frequency{You Have Earth Quake Vibrate Or Soil Vibrate Identifying Machine And You Can Easily Identify Them} Which Go Under 500 Kilometer Of Soil {Under Soil Surface}{They Are Under Soil Surface But Not So Under Deep Soil Surface}Than You Must Be See Them Those Places .And You Should Get Words About Gog And Magog(Bibel) That Yajuj And Majuj(Quran) In Quran And Bibel.Creator Send Them Long Thousand Years Ago Under Soil For Their Cruelty.And They Are Originally Cruel Caste.Its Your Matter You Are Believe Or Not.He Created Everything And He Knows Very Well About Creation.He Is Mine And Our Creator.We Are Nothing.Who You Are Donot Know About Gog And Magog(Bible) That Yajuj And Majuj(Quran) You Can Just Give Internet Search And Get Knowledge About Gog And Magog(Bible) That Yajuj And Majuj(Quran).

New Basic Color Making Procedure From Plant(Like Heaven Color)……………….. New Basic Color Making From Plant Main And Important Matter Is Color Contrast…….And Here How Color Will Be Contrast…………..Color Contrast Main And Important Matter Is We Should Be Hold Whole Plant Or Branch Or Leaf By Rope And Stick Or Fence For No Movement…….Without Color Will Not Contrast…….That There Will Be No Movement Of Leaf Or Plant Or Branch Without Color Will Not Contrast. So…We Should Be Work On Unexpress Color Pigment And Sunlight Color Wavelength Change By Mirror Device…..If Sunlight Color Wavemength Suppose Green Color Wavelength 25nm And If We Are Change With 50nm 70 nm 75 nm 100nm Than New Basic Color Will Be Come And Bloom At Plant. We Can Push Others Color By Injection Syringe At Plant Soft Bark Or Leaf Or We Can Work Just Like Paint Others Colors By Painter Without Erase Soft Skin Elastic Or With Erase For Beautification Or Others Or We Can Cover Leaf By Cloth Or Others For Somedays To Make White And When We Will Give Cover Than After Somedays It Will Be Whitish Color And After That We Will Be Give Color Or Just Paint Color On Leaf And Branch And Root…..And If We Are Cover Plant Or Little Plant By Poly-Ethylene Normal Polybag That It Will Be Change Sunlight Color Wavelength And After Follow Some Procedure And If We Are Push Others Color Or Paint Color At Plant Than Cover Plant Or Little Plant By Poly-Ethylene Normal Polybag For Somedays Than After Somedays It Will Be Various Colorful Croton Plant And Normal Green Plant Will Be Various Colorful Croton Plant….By This Way We Can Also Change Sunlight Color Wave Length And Make Green Plant Various Colorful Croton Plant And Also We Can Make New Basic Color

Corona Mutation Pathogen: Corona Symptom…..Taste Less,Fragrance Less,Weakness,Gut Problem,Inspiration Problem,Liver Problem,Fever,Body Pain And Others………….Those Are Mutation Pathogen Symptom And Those Are Not Virus Diseases Symptom….Though Pathogen Type Diseases Comes For Virus And Bacterial Infection But Cure System Different…….Here Corona Spread From China……..Maximum Chinese Eat Half Boil Food……Corona Spread From Sick Mammals Or Flying Mammals……Animals Eat Maximum Times Rotten Food…..At Rotten Food Various Types Of Germ, Virus And Bacteria Lives That Various Pathogen Lives……When You Will Be Not Properly Temperature Cooking Food And Eat Half Boil Food And Eat Than It Should Be Sick Animals Inside Germ Should Not Be Die And By Half Boil Eat Food Sick Animal Inside Diseases Germ Should Be Enter At Your Body And You Should Be Attack By Diseases Germ And Gonna Sick…..Like That Half Boiled Sick Mammal Or Flying Mammals Eaten By Chinese And Corona Pathogen Enter Chinese Body And Like Influenza Its Spread All Over Earth And Kill People.To Take Corona Sampol And If You Are Exam Pathogen Diagnostic Lab Than You Will Be Easily Sure That Corona Is A Pathogen And Not Virus….Just Exam Its Sampol.If You Are Simply Eat Taro,Coconut Fruit And Water,Vit-D,Sunlight Than Corona Pathogen Will Be Ultimately Ok…..Cause Taro,Coconut,Vit-D,Sunlight Has Usefulness Against Pathogen Type Diseases…..To Take Corona Sample At Pathogen Diagnostic Lab We Can Easily Prove Corona Is A Pathogen Not Virus

Every Fruit,Vegetable,Plant Or Leaf Has Medicinal Value…………….Creator Gives Every Diseases Cure And Remove Solution To Every Fruit,Vegetable,Leaf…………

Condition:You Should Be Eat Regularly Those Fruit And Vegetable Than Those Fruit And Vegetable Chemical Will Be React With Body And Disease Germ And After Certain Time It Will Be Kill Diseases Germ And Body Will Be Good And Ok And Antibody Will Be Arise Also At Body

Suppose: Diabetes Disease Solution……..Malta Fruit(Blood Organge) And You Can Also Eat Garlic,Orange,Lemon,Ginger And Vit-E And Flattened Rice(Poha) When We Put Water For 15 To 20 Mintutes And Than We Will Be Take That Water And Eat That Water And Fish Head And Leaf Vegetables

Body Fat Removal--------Apple,Cumin,Elachi,Water Melon,Jack Fruit,Carrot,Potato,Lettuce,Ginger,Lemon,Pumpkin,Taro,Cabbage,Cauliflower

Corona Pathogen:Taro,Coconut Fruit And Water,Vit-D,Sunlight

Good Skin:Mango,Jack Fruit,Pumpkin,Cabage,Taro,Sandal Wood

Like That You Should Be And Must Be Get Every Fruit,Vegetable,Plant Or Leaf Has Medicinal Value…………….Creator Gives Every Diseases Cure Or Remove Solution To Every Fruit,Vegetable,Leaf But

Condition:You Should Be Eat Regularly Those Fruit And Vegetable Than Those Fruit And Vegetable Chemical Will Be React With Body And Disease Germ And After Certain Time It Will Be Kill Diseases Germ And Body Will Be Good And Ok And Antibody Will Be Arise Also At Body

Is Air Particle Or Ripple Wave?.....Is Light Particle Or Ripple Wave?......Is There Air Or Light Has Weight And Mass?....Here Water Is Liquid…..But Water Can Be Solid Or Gas……Just Like That Air Is Wave But If We Wanna We Can Make Air Solid Or Liquid…..Now How? Here Environmental Light,Cloud Movement,Plant Movement Depends On Air…..But At Environment Various Types Of Partial Reaction Happen Everytime…..And Various Types Of Frequency Happen At Environment…..By Air And Moments Maximum Heavy Wave And Acceleration At Environment Goes To Around Earth Or Planets…Air Has Mass And Weight? Now How We Can Identify…..You Know People Use Air At Vehicle Tyre….If You Are Take Mass And Weight Of Vehicle Tyre Before Push Air And After Push Air Than You Must Be Get Difference And After Push Air Tyre Weight And Mass Increase And That Is Air Mass And Weight…..So….It Must Be Air Has Weigh And Mass And Every Air Atom Has Mass And Weight….Like That Light Wave Has Also Mass And Weight And Every Light Atom Has Mass And Weight(If We Are Exam Light Just Like Air And Vehicle Tyre) And All Wave And Particle And Aton Has Mass And Weight….And If We Want Than We Can Make Air And Light Solid,Liquid,Gas Or Wave……Here If We Want Than We Can Make Air Or Light Crush That Pulverize Than Environmental Everything Will Broken Just Like Broken Glass And People Animal Plant And Their Body,Blood,Hand,Leg,Head Will Be Scattered Just Tear Down That Tattered That Broken….Air And Light Will Be Kill People Just Like Archer If We Will Crush Air And Light….If We Are Use Some Procedure Than We Can Crush Air And Light Easily…And Environmental Atom And Free Atom And Its Charge Gonna Charge And Their Charge Arise With Seasonal Air And Change With Seasonal Air With Light And Time But…..By Air And Moments And Light Maximum Heavy Wave And Acceleration,Particle At Environment Goes To Around Earth Or Planets ……And Here There Are Various Types Of Particle,Wave At Environment To Control Climate And One Is Flemingo Particle…..And This Flemingo Particle Maximum Control Environmental Climate…..And Flemingo Particle Absorbs Environment Extra Everything……When Flemingo Particle Gonna Inert Than Environment Carbon,Temperature,Storm,Tsunami Increase….Though Environmental Temperature Depends On Light But Increase Or Decrease Or Environmental Abnormal Temperature That Heavy Temperature Or Low Temperature Depends On Flemingo Particle… Climate Control…….You Can Simply Work On Flemingo Particle……Flemingo Particle Is One Kind Of Particle Which Blows Or Flows At Environment,Soil,Water,Air.And Environment Temperature,Carbon,Partial Reaction,Tsunami Storm Depends On Flemingo Particle……Flemingo Particle Absorbs Environment Extra Everything…When Flemingo Particle Gonna Inert At Environment Than Temperature,Carbon,Tsunami And Others Gonna Increase………We Can Work Just On Flemingo Particle From Inertness By Electric Wave And Resonance Reaction And Work On Flemingo Particle That Environment And Climate Will Be Ultimately Ok…. According To Theory Of Relativity------Light Velocity Is Constant---------If Light Velocity Is Constant Than Why Temperature Gonna Different?Why Seasonal Change(Summer Winter Rainy Season And Others Seasonal Change Happen?----We Know Plant Takes Light To Make Food-----So,If Light Velocity Is Constant Than Why Short Day Plant-Long Day Plant And Day Neutral Plant-Seasonal Vegetables-Fruit-Flower Comes When Season Change?And When At Open Field Where There Is No Obstacle Of Light And There Also Short Day Plant-Long Day Plant And Day Neutral Plant-Seasonal Vegetables-Fruit-Flower Comes When Season Change.So,Light Velocity Is Never Constant----It Can Be Light Spped High But Truth Is Light Is Ripple Which Goes By Master Force--- Now What Is Master Force?Master Force Is One Kind Of Force Which Flows Or Blows All Over Universe Within Sometime Just Like Wind Flows All Over Earth.And Light,Wind And Others Velocity And Intensity Flows Or Blows By Master Force.Example:Suppose Sea Water Wave Or River Water Wave Blows By Wind And Its One Kind Of Ripple And Light Is Also Ripple.If Wind Is Just Like Sea Water Wave Then Master Force Is Just Like Wind.By Master Force Light,Wind And Others Intensity And Velocity Pass Planets And All Over Universe.Now Why Master Force Comes?----Its Cause Planets Rolling In All Over Universe And Rolling Planets Has Scale Of Balance For Rolling Balance.And For Rolling,Absorb,Spark And Others Cause Master Force Comes And Blows Or Flows All Over Universe.Why Your Sky Color Is Blue?I Guess,Its Cause Your Plant Leaf Color Green,Soil Color Ash Or Red And Sea Water Blue.So,Light Velocity Is Never Constant……… We Can Work Just On Flemingo Particle From Inertness By Electric Wave And Resonance Reaction And Work On Flemingo Particle That Environment And Climate Will Be Ultimately Ok….Or We Will Be Crush Flemingo Particle From Inertness By Resonance Reaction Than Earth And Environmental Climate Will Be Ultimately Ok….Is Those True About Work-Energy And Kinetic Energy Equation- (Speed=Utter Energy*Velocity That S=UV Equation)-Mirage Equation And Others Equation?....Please See Attachment For Further Discussion

please tell me If there is a standard ratio in rearing Tilapia fish in an aquarium.

I am developing a UHPLC method for content and purity for a peptide based on 100mM KPF6 and 100mM NH4PF6 pH 3.5. TFA as an ion pair reagent does not give the required separation performance. My question is less about method development HPLC for peptides, but more about the negative effects of KPF6/NH4PF6 in the UHPLC systems used (Waters IClass binary system and Thermo Vanquish Horizon binary system).

The negative effects on 3 instruments were already noticeable after 1 to 2 sequences (extreme pressure fluctuations):

The repairs carried out showed more or less the same damage:

- strongly porous seals in the pump heads

- damage to the pistons

- defective inlet/outlet check valves

One system was used over 2-3 months and showed the most damage.

I will ask my questions right at the beginning:

- What experience do you have in using KPF6, NH4PF6 as additive/buffer in mobile phases for UHPLC analysis or for method development for peptides?

- How can these reagents be used "safely" and robustly without causing damage to the pumps?

These chaotropic reagents seem to be highly corrosive. I would be glad to get some useful advice. Many thanks in advance for your support and advice.

Kind regards

Ronald

Mobile phases are:

Mobile phase A 0.1M KPF6 pH 3.5 / ACN 8:2 (V/V)

Mobile phase B 0.1M KPF6 pH3.5 / ACN 35:65 (V/V)

and

Mobile phase A 0.1M NH4PF6 pH 3.5 / ACN 8:2 (V/V)

Mobile Phase B 0.1M NH4PF6 pH 3.5 / ACN 35:65 (V/V)

Preparation:

0.3M Buffer KPF6 pH 3.5

• 55.2 g KPF6, were weighed into a 2000 mL beaker. 1000 mL water was added and stirred until complete dissolution for 15 min. Sonification for approx. 2 min. It was adjusted to pH 3.5 with Orthophosphoric acid 85% (+/- 255 µL) and stirred well. The buffer was filtered with a Steritop-GP 1000 mL Express Plus PES 0.22 µm into a 1000 mL bottle.

Mobile phase A1: 0.1M KPF6 pH 3.5 / ACN 8:2 (V/V)

• 330 mL 0.3M_ KPF6 buffer pH 3.5, 470 mL water and 200 mL acetonitrile was transferred into a 1000 mL bottle and mixed well. Sonnification for approx.5. min.

Mobile phase B1: 0.1M KPF6 pH 3.5 / ACN 35:65 (V/V)

• 330 mL 0.3M_ KPF6 buffer pH 3.5, 20 mL water and 650 mL acetonitrile was transferred into a 1000 mL bottle and mixed well. Sonnification for approx.5. min.

0.3M Buffer NH4PF6 pH 3.5

• 48.9 g NH4PF6, were weighed into a 2000 mL beaker. 1000 mL water were added and stirred until complete dissolution for 5 min. It was adjusted to pH 3.5 with 25 % NH4OH (+/- 340 µL) and stirred well. The buffer was filtered with a Steritop-GP 1000 mL Express Plus PES 0.22 µm into a 1000 mL bottle.

Mobile phase A1: 0.1M NH4PF6 pH 3.5 / ACN 8:2 (V/V)

• 330 mL 0.3M_ NH4PF6 buffer pH 3.5, 470 mL water and 200 mL acetonitrile was transferred into a 1000 mL bottle and mixed well. Sonnification for approx.5. min.

Mobile phase B1: 0.1M NH4PF6 pH 3.5 / ACN 35:65 (V/V)

• 330 mL 0.3M_ NH4PF6 buffer pH 3.5, 20 mL water and 650 mL acetonitrile was transferred into a 1000 mL bottle and mixed well. Sonnification for approx.5. min.

After sequence, the systems were flushed with

3.43 ml acetic acid and 3.26 ml isoamyl alcohol were reacted and the weight of isoamyl acetate was weighed at 2.114 g using balance.

The entire globe is facing a problem through the climate change. We all know that the climate change and global warming is an outcome of irresponsible human actions. Countries have signed the Paris agreement and are working to accomplish the net zero target at their best.

There are two arguments about the net zero target accomplishment. First, reduction is consumption which reduces the production, transportation, use of energy and ultimately reduces the emissions. Second, financing carbon neutral, green, clean and sustainable projects will gradually reduce the emissions.

Given these facts, which one do you think weighs more to help the environment and to accomplish the global net zero target?

We have a small evidence on the financing side (https://blog.bham.ac.uk/business-school/2023/03/28/green-bonds/) and working for causal evidence. I look forward to your thoughts for discussions.

A 10 X 10 three-story precast building with a floor area of 100 m2 with 30 cm thick concrete walls in a large earthquake with an acceleration of 1.5g has some overturning moment tendency.

Wanted 1. how much does the precast weigh

2.the inertia and shear base created at the 1.5g acceleration

3. plus the magnitude of the overturning moment of the entire building.

The building weighs 340 tons

Payload is 80kg/m2 = 24 tons

The floors another 24 tons

The building finally weighs 340+24+24= 388 tons without the base.

Inertia and base intercept = mass X acceleration = 388 ton X 1.5g = 582 tons

Overturning moment = height X inertia = the first floor is 3 m high the second 6 and the third 9 total 3+6+9 = 18m

Each of the three floors has an inertia of 582/3 = 194 tons

18mX194ton = 3492 ton overturning moment

But the precast as a rigid structure has a double lever arm, that of the height and that of the width.

So we divide the torque of 3492 tons by the width of the building which is 10 meters 3492/10 = 349 tons.

Every single anchor I have can withstand 100 tons of torque at two meters depth.

If we place 8 anchors around the perimeter, we will not have any loss of support from the ground due to the total withdrawal of the area of the base of the building, so no damage in the earthquake of 1.5g acceleration.

A 300 sq.m pre-fab house costs 310,000 euros finished today + 30,000 the eight anchors = 340,000 euros and you have the most earthquake-proof house in the world.

A conventional house today costs 2,000 euros per sq.m when finished, 300 sq.m costs 600,000 euros.

Choose.

https://www.youtube.com/watch?v=XsHC9WJwgyU

My proposal for anti-seismic constructions is to prestress the sides of the reinforced concrete walls as well as to compact them with the foundation soil at the same time.

Prestressing + compaction on the sides of the walls Prestressing (generally compression) on the sidewalls has very positive effects, as it improves the oblique tensile trajectories. On the other hand you also have the other good thing... the non-shear failure of the cover concrete due to the high tensile strength of the steel, the reduced cracking and the increase in the elastic and dynamic displacement area due to compression, which increases the effective cross-section and stiffness of the structure, and most importantly increases the response of the cross-section to the intersecting base. If prestressing is combined with compaction in the soil then we divert the seismic loads into the soil, prevent the moments at the nodes, control the eigenperiod and ensure soil samples and a strong foundation.

I weigh 100 mg of tissue. I don't cut open the tissue and wash with room temp PBS. I use 300ul ripa while homogenisation and add 200ul after that.

MSE error weights both positive and negative errors equally. Is there any way to weigh them differently and create a new type of error ?

Hello everyone,

To produce a composite material, how will I weigh the components mentioned above if I want to make this mixture?

H₂Cit^------H⁺+HCit^2- pka 4.67

HCit^2------H⁺+Cit^3- pka 6.40 I am supposed to choose the pka closest to my buffer pH, so I can use pka 4.67 for calculation. I will need acid form H₂Cit^- and basic form HCit^2- to make my buffer, right? But if I only have citric acid (H3Cit ) and sodium citrate (Na3Cit), which means I only have H3Cit and Cit^3-, so I can't weigh what I have to make the buffer, right?

I know I can make 0.1M citric acid and 0.1 M Na3cit to reach the pH, but I want to know if I can make citrate buffer by weighing certain citric acid and Na3Cit directly... I hope you understand my question. Thank you in advance.

Hi so I'm trying to get to a concentration equivalent of 1ug/ml in a volume of 0.1 ml but i cant weigh out the amount of solute due to the limitations of the balances in my lab being only analytical. Is there a way i can use dilutions to get to these such small concentrations?

I'm doing this because i accidentally bought well plates with a volume that was not equal to the one i based all of my calculations on.

I am working with dry ground leaf material. This is the extraction protocol.

1. Weigh 25 mg of ground leaf powder, add 10 mL solvent to obtain 2.5 mg/mL extract

2. The extract is diluted 5000 fold to obtain 500 ng/mL

3. After analysis using LC/MS and using the standard curve, the amount of compound X is 46 ng/mL.

4. I want to convert the 46 ng/mL of compound X to mg/g of dry extract.

Please advise on how to do this.

Thanks,

Ruth

I'm trying to make a mixture of Gallium oxide (M.W = 187.44) and Tungsten Oxide (M.W = 231.85). How can I make a composite of these two different chemicals based on their molecular weights by taking equal amounts of the individual chemicals?

We purchased Nitinol wire on Amazon. Now, we would like to know if anything (any heat treatment) needs to be done before utilizing the wire for its intended purpose of shape memory effect? The intended purpose of shape memory alloy is to grip objects that weigh around 50 grams.

We tried heating the as received wire to 50 degrees celsius. Nothing happened.

Request experts to provide suggestions.

The reason of doing twice, I noticed that my sample was not quit dry yet after 24hrs of lyophilizing. The first time was 3ml of 50:50 (t-BuOH:ddH2O). When I weighed the sample, I figured out that its mass was not quite right, and that is why I want to relyophilize for longer time. So I don't if it Ok to lyophilized back with the same liquid (50:50) t-BuOH:ddH2O.

Thank you

I am trying to obtain the confidence interval (CI) of the standard deviation of several weighted beta coefficients from linear and logistic regressions.

In Stata, I can obtain the CI using the "nlcom" command. However, the command is very slow, especially if you weigh the coefficients.

In MPLUS you can use the "constraints" command to estimate the point estimate and CI of combinations of parameter estimates from a model.

However, I have to weigh these parameter estimates by the proportion of the population e.g. imagine we have dummy variables for different types of religion and I want to weigh the Betas of these dummy variables by the proportion of people who follow each religion.

I want to automate this process so I do not have to write the weight [proportion] for each beta coefficient in the "constraints" section of the MPLUS input file.

I got the mass lost data and temperature already, but the lab assistant did not take the same amount of samples, so the curves don't start from the same origin which difficult to compare them. Now, I want to change the data of mass to weight percentage.

thank you

When AHP is used for calculation of weights only and SAW is used for ranking the alternatives , how to carry out the sensitivity analysis ?

We are thinking about getting a EEG system, so we are weighing all the different specifications. Many systems offer sampling rates of up to 80,000hz, what is the advantage of having such high sampling rates when the highest signal frequency that we would observe in the brain is around 100hz?

I am working on code development for compressible flows and i am stuck on some basics. I want to understand that in a standard D2Q9 Lattice is there 9 particles or just one particle that can move in any of the 9 directions depending on weighing factor?

I have been performing experiments in which I weigh polystyrene or polyurethane in an analytical balance, but anytime I place my sample, the weight keeps going down and does not stop. I read that probably is a problem with the static of the sample. Has anyone encountered this problem and knows how to deal with it?

Thank you

I have rubber formulation in phr. To know the amount needed for that formulation, I am confuse to weigh using weighing balance or measure in volume since my additive are in dispersion (4 additive) and some are in solid (2 additive). Can I directly weigh these two additive which in solid form and combine with other that are measure in volume and mix together ?

Hello, I tried to produce polymeric nanoparticles. I synthesized polypropylene adipate to realize a hybrid system with branched PEI. I used PVA (6000) as a surfactant in the double emulsion solvent evaporation method. pPAd:PEI (mg:mg) was weighed and dissolved in 5 ml of DCM.

The problem:

1. I tried PVA concentration from 1% to 8%

2. I tried all pPAd:PEI ratios (5:45, 10:40, 15:35, 20:30, 25:25, 30:20, 40:10, 45:5)

As a result, under pPAd( 40:10) and PVA: 1% conditions, I measure the size at 150.1 nm and the PI value as 0.1, but after 48 hours the particles precipitate into aggregates. Particle size and PI are excellent for my work but aggregation is a big problem. Could you help me to solve this problem?

I've been trying to amplify a plasmid that I'm running low on, but every time I complete the purification step and try to run the products on an agarose gel, the samples float out of the wells. I've tried making sure to remove any remaining wash solution (containing ethanol) but that did not help, and I've run the samples on a nanodrop to make sure they contain DNA.

I've heard that using a glycerol-based loading buffer can help weigh down the samples on the gel, but I was wondering if I could just add glycerol directly to a pre-existing loading buffer (this one here specifically: www.neb.uk.com/products/neb-catalogue/other-products-(cloning)/gel-loading-dye,-purple-(6x)).

#balance_scale #lyophilization #weighing

For my PhD, I am measuring glucocorticoid metabolite levels in flying squirrel feces. I have freeze-dried the fecal samples and am now at the stage of crushing them (using a mortar and pestle) so that they can be weighed. The desired weight is 0.05g, but I am finding that a lot of the samples are smaller than this. On top of that, the process of moving the sample to a mortar, pestling it, and moving it back to the tube is resulting in lots of lost sample (i.e., ground sample is getting stuck to mortar and pestle because of static). I have heard that some people use a micropestle to grind the samples directly in the tube to overcome this. Have others found this to work well? Does the sample get sufficiently ground for glucocorticoid analysis? Do folks have any other tips?

Thank you!

Hello!

I am going to dissolve phenylalanine and phenylpyruvic acid in the medium.

It is a very small amount of about 2mg, so there is a lot of loss when weighing it.

Then, I want to make a high-concentration stock in advance and dilute it before use.

To do this, I'm trying to freeze the culture medium, but is it still stable?

We also considered dissolving it in PBS or DMSO. However, I found that the pH of PBS changes after freezing, and DMSO is cytotoxic, so I am concerned.

I look forward to the help of many people.

I'm sorry my English is not good.

Thank you!

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can anyone weigh in on the quality and their experience? TIA

Hello,

I am having some trouble deciding the accurate way to calculate my initial extract concentration. This is what I have done:

1) I weighed my empty urine cup.

2) I weighed 1g of my plant and placed them in the empty urine cup and I performed my extraction process that involved soaking the plant powder with the solvent, then centrifugation, filtration, and evaporation, and I was left with the concentrated plant extract.

3) I weighed my urine cup afterward and subtracted it from the first weight and was left with 100mg of plant extract

Do I consider now using my initial powder weight (1g) as my initial concentration and reconstitute it with my solvent (for example in 10ml of methanol and the concentration becomes 100mg/ml)?

Or do I use the 100mg that I obtained after evaporation and use it as my initial concentration (for example 1ml of methanol and the concentration becomes 100mg/ml)?

which is more accurate?

Thank you!

I collected daily feces from two mouse groups and stored them in -80°C. Now i'm prepared to compare TG content in feces of two groups following a method which was entitled "Lipid Extraction from Mouse Feces" (this method was published in Bio Protoc. 2015 Jan 5; 5(1): e1375.).

1. Dry the feces at 60°C overnight about 11 hours (this step is personally added)

2. Weigh exactly 1000mg of dry feces per mouse and powderize it using the tissue grinder.

3. Add 5ml of normal saline to 1000 mg of powderized feces in a 15-ml tube and vortex.

4. Add 5 ml of choroform: methanol mix (2:1 by volume)and vortex.

5. Centrifuge the suspension at 1000 × g for 10 min at room temperature. Afterwards a phenomenon that two liqiud phase seperated by a solid phase will happen. The lower liquid phase contains the extracted lipids in chlorofoem:methanol mix.

6.Use needle to drain out the lipid containg lower liquid phase.

7.Place the collected lower lipid phase in a stand under a fume hood, leave the tubes alone for 3-4 days until all liquid is evaporated.

8.Analyze lipid mass of feces in grams.

TO assess lipid concentration

9.Dissove solid lipid in 1ml isopropanol. Use Wako LabAssayTM Triglyceride(GPO ･ DAOS method) to test TG .

I have several questions and will

appreciate some advice from more experienced people.

Q1: After adding choroform: methanol mix (step4 ), how long will it takes to completely extract lipid from grined feces? Is it necessary to use a shaking platform for overnight?

Q2: when lower liqiuid phase drain out from the pore (step6), some granulum from solid phase may also come out which will greatly influence lipid mass analysis. Is there some advice?

A lysimeter decreased in weight by 120 kg over a period when irrigation and rain was 30 mm. What was the evapotranspiration (in mm) if the area of the lysimeter was 1 m² an the height 0.8 m ?

My query is, has anyone worked with the compound, I got a 250 mg bottle from SIGMA. But I will only need about 70mg and planning to store the rest for further use.

Should I weigh and resuspend per use? or resuspend the whole amount and aliquot ?

The chemical is very light sensitive and that's why I ask if someone can help me with this.

I have seen that in Gekko gecko they use 1 volt to obtain seminal samples, but I search for Phyllodactylidae individuals that weigh 6-7 grams and measure 5-7 cm.

I am interested in the causes of war and wish to investigate the role of alliances in causing wars via case studies. My current hypothesis is that as alliance networks tighten between countries, war becomes more likely. What might be a typical, deviant and critical case to study on this topic? If possible, can you also explain why you think each case you suggest is a good case study for me (so I have something to weigh on for my research). Please do help me in this regard.

I am trying to build a conveyor that weighs the products while moving, but there are several challenges:

1- As the conveyor is big , I am using four load cells at the corners.

2- the conveyor is too fast, the sensor must have low time response.

3- the accuracy of the available load cells is not that sufficient.

My question is : is it possible to train a neural network with the used weights, so when the four load cells detect certain oscillation they predict the right weight?

Thank you,

I have in my lab TCA bottle. It is used and still ca. 200 g. However, i notice that it might contain some water because it is hygroscopic.

I want to prepare 20% TCA solution to be used for protein precipitation. I work on marine invertebrate.

I tried yesterday: I weighed 22 grams. Under fume hood, I put TCA in a glass dish and heated below using a hotplate for 1 hour. I noticed that it turns into dark yellow fluid and recovered 3 ml only (almost 4 grams).

22 - 4= 18 grams lost. Do you think this amount was water. Or do you think much of TCA has evaporated as well.

Thank You

It is related to plant anti-nutrient analysis.

Method says:

The oxalate content was determined by the method of titration as described by Idris et al. (2019). A gram of the pulverized sample was weighed into a volumetric flask containing 75 mL of 3M H2SO4, and the mixture was stirred with a magnetic stirrer for an hour. The mixture was filtered, and 5 mL of the filtrate was transferred into a conical flask, and titrated against 0.05M KMnO4 until a reddish-brown color persists marking the endpoint. The oxalate content was calculated and expressed as 2.2 mg oxalate as an equivalent to 1 mL of 0.05 M KMnO4.

I need to add 4 volumes of 5% MPA as a stabilizier to red blood cell pellet (after plasma separation). Can someone help me calculate what this 4 volumes mean? Will I need to weigh the RBC and then multiply that with 4 to understand the volume of MPA?

e.g. if I have 1mg of RBC pellet, I add 4 mL MPA?

Thanks!

Hi everyone, I have purified my bacterial protein weigh 22kda after purification I start concentrating it but after concentrating a few ml my protein start precipitating aven I have kept my ph 1 unit above the Pi of protein. Any suggestion?

Samples held in plastic beakers have a lot of static electricity after freeze-drying, which makes it difficult to weigh the samples and will waste a lot of samples, what is the way to remove the static electricity from the samples?

I am conducting research of stomach content of fish that mostly larvae. However, i can't get the value of Fullness index as most fish are small so that the stomach contents can't be weighed and i have only 0.0001 precision weighing scale

Hello,

As the question states, I intend to automatically log the data from my digital balance at a set time interval (if a set time interval creates too much complexity then I am fine with the default rate).

Most guides I have seen online are sort of semi-automatic where I would need to press the 'print' button on the scale in order for the balance to transmit the mass data to the computer. Is there any way for this to be automated so I do not have to sit and press the 'print' button for every reading.

Any help would be appreciated.

Thank you

Vancomycin powder product detail states

potency  1023 μg per mg (anhydrous basis)

impurities  ≤10% water

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample on a filter) I previously tested are not very accurate. In our lab we only have the ability to measure absorbance and fluorescence, do you have any protocols I could try?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology and would appreciate more seasoned individuals weighing in. I've provided unstained microscope images of specimens at various levels of magnification as attachments. Though to be honest, I'm not always confident in my ability to tell the difference between specimens and bubbles/dust/fibers that could be contaminating my slides. The last three pictures were taken on a higher powered microscope with the assistance of more senior faculty.

If interested, I would be happy to share more images upon request.