Laboratory Medicine - Science topic

Hi folks,

A niche question, but does anyone have a recommendation for scientific journals with a focus on the place laboratory medicine holds within crisis/disasters/global health apart from the traditional larger journals?  

To be precise, I am interested in those that air thoughts and research at the intercept of diagnostic laboratory medicine and health systems/policy/preparedness in the context of crisis and disasters.

I've been wondering whether there are any new manufacturers around who make reliable, affordable PCR machines. The Chinese now make great smartphones, may be they now make good cyclers, too (that cost way under $5,000...) Any suggestions?

I am looking for resources/ publication on systems model please; anything of that sort I'll greatly appreciate. Thanks, Nellie

I want to know the allowable CV for the hormone assays.

Hello,

We are centrifuging fresh blood to obtain serum. The blood is collected in plain, uncoated vaccutainers and allowed to clot at room temp for 30-45 mins.

Several method papers have suggested 1.0-1.3 RCF (1,000-1,300 g), including a consensus paper. However, other labs i have asked go up to 2.0-3.0 RCF on a regular basis.

I was wondering what procedure everyone else used and what considerations go into determining centrifuge speed (haemolysis, sample separation and so on).

Dr. I. Trowbridge (Immunobiology Research Center, Departments of Laboratory Medicine/Pathology and Surgery, University of Minnesota, Minneapolis, MN 55455, USA) is not part of the university staff and the TTO cannot locate the hybridoma

Hi, I'm trying to fractionate blood components by density centrifugation. I've been able to clearly isolate the monocyte/lymphocyte layer, however the granulocytes tend to drift off into the packed RBC layer. The protocol includes using 100 ul of blood in a glass capillary tube and centrifuging the sample at 1500 RCF for 5 min.

Any suggestions on how to prevent this from happening without the addition of density gradient medium? Is it because the the centrifugation force is too high or the centrifugation time is too long?

Thanks for your help!

Colorimeter/spectrophotometer, autoanalyzer, and immunoassay changed the field of diagnosis when introduced. What are the current advances in the use of mass spectrometry in diagnosis and what is the future of this important technology in Clinical Chemistry.

The dose is standardised in animals in mg/kg.

i just wanna extend the same treatment to cell lines and was trying to find a way to convert Drug dose from mg/kg to mg/ml ?

Preanalytics

In a collection of human blood chemistry data the complete lipid profile and atherogenic index shows residue of obesity. Along with this I found detectable changes of their electrolyte values too. My question is how I can correlate edema with obesity of their data?

Can the remaining platelet number in plasma be the reason?

Is there a way to avoid completely clotting activation when thawing plasma?

A report by the FDA difference hsCRP and cPCR for their requirements. Do you think that this difference is real?

I am asking this because there is a significant differences between plasma & serum in electrolyte assay, can anyone help me? I am using fish.

For metabolic evaluation of kidney stone formers 24 hours urine collections are still needed.

How do you suspect 24 h urine collections are not appropriate? With a formula?

Does anyone add a commentary in laboratory reports, if the collection is not appropriate ?

I need information about the right amount of the reagents and sample

New national guidelines on the diagnosis of diabetes advocate the use of HbA1c as the test of choice. A levels of >48 mmol/mol is consistent with diabetes. The guidelines state all positive tests should be repeated within 14 days to confirm result. We are seeing quite large disparities between first and second test result (much bigger than can be attributed to analytical variation). This could be regression to the mean or genuine rapid shifts in glycaemia (being told you may have diabetes leading to changing eating/ exercise habits etc).

I would be very interested to know the biological variation of HbA1c and what change we would expect to see due to normal physiology. Any thoughts would be appreciated!

Do you use in your laboratory practice specific reference intervals for laboratory tests made ​​from capillary samples or apply the same reference intervals that you use for samples from a vein (serum / plasma)?

Biological variability

There are many minerals, vitamins and other food constituents that we need for optimal health. Sufficiency or excess of many key nutrients and other aspects of human nutritional status can be assessed through the analysis of biological samples. If you want to know about the nutrition status of your research participants, patients or clients, which nutrients and nutritional conditions are most important to you. To make it clear, the goal here is not to assess one or two specific nutrients because of a particular research question or prompted by clinical symptoms. This is strictly for global, non-targeted nutrition assessment. The preferred portfolio of assessment indicators will obviously depend on the target group and area of interest, so it would be helpful to hear about the rationale for the selection.

Is there a central phlebotomy service provided by the department of laboratory medicine?

How important are biobank facilities for your particular research? If you request biomaterial: do you have to pay any access fees?

I want to use ibuprofen as Non-Steroidal AID in dosing experiment for Oxidative stress. Need help how to dissolve it.