Labeling - Science topic

The waviness of these lines may just be due to the limited number of pixels available in the display. If you plot the data in a graphing program, such as Excel, are the graphs also wavy?

Emily Tsui You can choose Nucleic Acid application and pick SYBR, because the FAM molecule and SYBR have a similar ext/emm wavelength.

Dear Jitender

If it is feasible will you be able to share the MS.

Thanks

Rakhee

Scott, I am very grateful for the contribution of information, it will be substantial for the completion of our work!

For labeling cryo racks, I recommend using laser engraving directly on the metal surface of each rack. This method offers a permanent, highly durable solution that won’t fade, crack, or interfere with the carousel mechanism. If laser engraving is not an option, another alternative is using high-temperature-resistant adhesive labels specifically designed for cryogenic storage. These labels typically withstand extreme temperatures and are less likely to peel or crack. Combining these labels with a protective clear coating can further enhance their longevity without causing jams in the carousel.

Yes, you can but is should be ethanol-precipitated, see https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/277/135/11685597910.pdf?msockid=3c180f335eaa679411351d5e5f5b66f1

Hi Anastasia Kotlyarova

Please find the answers below.

The Ranker method in Weka ranks attributes based on their importance and allows you to select the top attributes based on either a threshold (cutoff value) or a specific number (numToSelect).

Your question is not clear, you may need to elaborate on it.

By default, PCA in Weka is designed to generate enough components to capture a large percentage of the variance in the data (e.g., 95%). This can result in more components than the original number of attributes because PCA aims to retain as much information as possible. Even though PCA typically reduces dimensionality, if the goal is to capture a high level of variance, it might generate more components to achieve that.

I hope that helps.

Kind regards,

Dr. Samer Sarsam

Detecting heptane indirectly after chemical derivatization (coding) typically involves converting heptane into a more detectable form, such as a product that is more reactive or has a distinct spectral signature.

Actually; Every antibody is specific for the specific antigen, Any antibody which is for specific antigen that will no work or bind with other antigen, because light chain of antibody have specific site for antigen and when we modify antibody it will lose its specific binding site , so ,you may use alternative antibodies or may there an error with the procedure of doing and change the experimental condition for working

Maybe the protein doesn't get sorted properly when there is a large protein tag on it. It might work better to use a small tag such as the ReASH tag system.

Thank you!

Narendra Dinkar Kharat

The replacement of a proton with deuterium is done to study the isotope effect in a chemical reaction. They study the replacement of a proton with some functional group, and then replace deuterium and compare the activation energies of the reactions.

Using dietary supplement ingredients in drug manufacturing requires careful regulatory navigation.

Dietary supplements and drugs are distinctly regulated; supplements don't need FDA approval before marketing but must be safe and truthfully advertised. Drugs, however, require rigorous FDA approval demonstrating safety and efficacy through extensive testing.If an ingredient from supplements is used in a drug, it must undergo clinical trials to prove its effectiveness for the intended therapeutic use.

Labeling standards also differ significantly; drug labels need detailed medical information, whereas supplements have more general nutritional information.Misclassifying a product can lead to severe regulatory penalties. It’s crucial to consult regulatory experts or legal counsel to ensure compliance with all applicable laws when considering such development.

Dear Manoj Kumar Srivastav ,

Previously, we described how to perform correlation test between two variables. In this article, you’ll learn how to compute a correlation matrix, which is used to investigate the dependence between multiple variables at the same time. The result is a table containing the correlation coefficients between each variable and the others.

Regards,

Shafagat

In the context of PCR and DNA amplification, 'carryover contamination' refers to the accidental transfer of previously amplified DNA (amplicons) into new PCR reactions. This contamination can lead to false positives, where the detected signal is due to these contaminant DNA molecules rather than the target DNA from the sample being tested.

I would try VGLUT and VGAT antibody from Synaptic Systems, wich are very good in labelling synapses. Both are presynaptic markers. You can also use Homer1 (post synaptic excitatory) or Gephyrin (post-synaptic inhibitory). Also in this case, the Synapt Systems ones, works really good.

Differing projections of the sensor optics. You can correct with a positional warp. This can be done by matching associated points and using a polynomial offset in X and Y. OpenCV also contains homography methods which can align different sensors.

Dear Dr. Leonor Huerta Hernández

No, it is better you don’t use Polyjet once it has passed its expiry date. You need to use a fresh vial. I never encourage the use of expired reagents. You will end up with unreliable results. Moreover, the experiment may have to be repeated which will only result in wastage of time and resources.

Regards,

Malcolm Nobre

Hi, Masoud Sadeghi

Yeah you can investigate more for the best insightful outcomes!

Pregnancy can never be deviant, because it is the law of nature. However, it depends on your economic situation, i.e. your resources and support to raise the child. Generally, society will punish you and the child, because you should have waited to have available resources to bring another human being being into this (unjust) world.

Sequential Excitation and Detection (Multi-tracking):

Excite and detect each dye sequentially rather than simultaneously. This approach ensures that the emission from one dye doesn’t interfere with the detection of another. For example, if you label particles with Alexa 488 (green) and Alexa 568 (red), excite and detect them one after the other to avoid crosstalk.

Spectral Separation:

Use additional spectral information to separate emission signals. If there’s overlap between the emission spectra of two dyes, choose laser lines and detection channels that minimize this overlap. Spectral imaging systems can help with this.

An Anthozoan species, which includes organisms such as corals, sea anemones, and sea pens, can be labeled as invasive if it meets the following conditions:Non-Native Origin: The species must be introduced to an area outside its natural range. This introduction can be intentional (e.g., through aquaculture or the aquarium trade) or unintentional (e.g., via ballast water from ships or through natural dispersal mechanisms).Establishment and Spread: The species must be able to establish a self-sustaining population in the new environment. This involves successful reproduction and spread within the new area.Negative Impact on Native Ecosystems: The species must have demonstrable negative impacts on the native biodiversity, ecosystem functions, or human activities. This can include:Outcompeting native species for resources such as space, light, and nutrients.Altering habitat structures and functions.Introducing diseases or parasites that affect native species.Disrupting ecological relationships, such as predator-prey dynamics or symbiotic associations.Human Health and Economic Impact: In some cases, an invasive Anthozoan species may also be labeled as invasive if it poses risks to human health (e.g., through toxins) or causes economic harm (e.g., damaging coral reefs important for tourism or fisheries).

From the viewpoint of quantifying mental health or mood science, depression is a mood disorder that causes a persistent feeling of sadness and loss of interest, i.e. the affected person is no more able to regulate her moods and the mental state of chronification can set in. Consequently, depression is a symptom as chronifying mental and physical response to a condition, e.g. learned helplessness.

Psychologists first described learned helplessness in 1967 after a series of experiments on animals, suggesting that their findings could apply to humans. Learned helplessness leads to increased feelings of stress and depression. For some people, it is linked with post-traumatic stress disorder (PTSD).

The most common treatment is therapy, especially cognitive behavioral therapy (CBT). CBT helps people overcome these types of challenges by changing how they think and act.

Ref/

_________

You Keri Mobbs wrote:

Depression used to be called meloncholia. After a guy won a nobel prize in conditioning, the world had two massive wars within years. Then it was labelled "the Great Depression".

Skinner, John B. Watson and Ivan Pavlov, are considered to be the pioneers of modern behaviorism. You seem to mean Ivan Petrovich Pavlov, Nobel Prize in Physiology or Medicine (1904)?

The inventor of the phrase, the Great Depression, is Lionel Robbins, a British economist who lived during the Depression. In 1934, after Hoover’s tenure in office, Robbins wrote the book, The Great Depression, which contains what some historians, notably David F. Burg, consider to be the fist usage of the phrase we now use to to describe the economic meltdown on the 1930s. Although Hoover did not quite invent the term the Great Depression, he did play a role in its formation.

Ref/

An expansion of bank credit creates a boom that cannot be sustained, and the inevitable collapse of the boom is the depression. Robbins traces this boom/bust cycle, detailing the central bank policies of the 1920s. If the policy gurus at the Fed had read and understood this book, they could have spared us the economic havoc of recent years.

__________

Keri Mobbs Economics is still very blind to the workings of human psychology as it uses linear models for accounting purposes, which are unable to understand the cyclical movements of human economic activity.

The mentioned Great Depression was, imo, a full central banking failure (systems error), which is also state failure (planning agency put over the market forces).

_____

PS/

There have been three great inventions since the beginning of time: fire, the wheel, and central banking. '' The reason Will Rogers made this statement is central banking is so important because the money supply is so important.

Dear Rardchawadee Silapunt ,

Data is the currency of the future.

With technology and AI slowly seeping into our everyday lives, data and its proper use can cause a significant impact in modern society.

Accurately annotated data can be used effectively by ML algorithms to detect problems and propose workable solutions, thus making data annotation an integral part of this change.

Regards,

Shafagat

May consider using Permai fluorescence dye.

I figured out the "problem" with my expression. So, my protein was labeled all along but the mass was lower than the expected mass (based on the sequence) because it was missing the N-terminal methionine residue.

I ran mass spec on an unlabeled sample of the protein and this was how I realized that.

Apparently, it is very normal for E.coli to get rid of the N-terminal methionine post-translation. https://www.nature.com/articles/326315a0.pdf

Suliman Khan

To find the eigenvalues of a matrix with quaternion entries in MATLAB, you'll need to approach the problem differently since MATLAB's built-in functions for eigenvalue computation (eig) do not directly support matrices with quaternion entries. One effective approach is to convert the quaternion matrix into a larger real matrix that can be handled by standard eigenvalue algorithms. Here's how you can do it:

Steps to Convert Quaternion Matrix to Real Matrix

Detailed Steps in MATLAB

Here's a step-by-step MATLAB code to perform the conversion and compute the eigenvalues:

matlabCopy code% Define quaternion units O = quaternion(0, 0, 0, 0); % Zero L = quaternion(1, 0, 0, 0); % One i = quaternion(0, 1, 0, 0); j = quaternion(0, 0, 1, 0); k = quaternion(0, 0, 0, 1); % Define the 4x4 quaternion matrix A = [O i O L; -i O j O; O -j O -k; L O k O]; % Function to convert a quaternion to its 4x4 real matrix representation quaternion_to_real = @(q) [ real(q), -imag(q), -jmag(q), -kmag(q); imag(q), real(q), -kmag(q), jmag(q); jmag(q), kmag(q), real(q), -imag(q); kmag(q), -jmag(q), imag(q), real(q); ]; % Initialize the real matrix n = size(A, 1); real_A = zeros(4*n, 4*n); % Fill the real matrix with 4x4 blocks for r = 1:n for c = 1:n real_A(4*(r-1)+1:4*r, 4*(c-1)+1:4*c) = quaternion_to_real(A(r, c)); end end % Compute the eigenvalues of the real matrix eigenvalues = eig(real_A); % Display the eigenvalues disp('Eigenvalues:'); disp(eigenvalues);

May consider using Permai fluorescence dye.

Please follow MDPI

Journal

Researcher Anirban sengupta and Azharuddin.

This CD4hi population is mostly antigen experienced (CD44+). You can also stain for CD44 and CD62L to distinguish naive vs antigen experience CD4hiD44hi CD4T cells.

In general, one of the commonly used storage conditions for azide-labeled proteins or peptides is to keep them in buffer solution at a low temperature, usually at -20°C or even lower, such as -80°C. This helps slow down any potential degradation reactions and maintain the integrity of the labeled molecules. In addition, storing them in the dark can prevent any photochemical reactions that may occur... On the other hand, storing azide-labeled proteins or peptides at low temperatures in a suitable buffer solution and protecting them from light should help maintain their preservation. Its stability over time.

Keri Mobbs First and foremost, one most significant aspect you should comprehend when it comes to seeking justice in accordance with international law in relation to your question is that a continent cannot physically sue or take any country to court. In order words, in terms of international law (UN Charter and ICJ Statutes), its is only possible if there are regional organizations such as; African Union or the European Union can mediate disputes or enforce treaties or agreements in line with the legal mechanisms comprises of the member states. In this note, base on your question there is no legal case at hand because such case never exist.

Some of the solutions are transfer learning, data augmentation, one-shot learning, ensemble learning, active learning, and continuous learning.

Infectious disease cause microbiological cause while cancer cassified according to thr aetiological agent

There are 2 main reasons why the protein could be precipitating: electrostatic interactions or hydrophobic interactions.

Increasing the salt concentration would probably overcome electrostatic interactions. You would have to remove the salt later, which is easy to do by passing the column through a desalting column or by dialysis. Changing the pH by several units could also help, but you have explained why this is a less desirable approach for you.

In my opinion, the more likely reason for the insolubility is hydrophobic interactions, since the interior parts of proteins are hydrophobic. You could probably keep the protein in solution using a detergent, but removing detergent later can be troublesome. There are a few detergents that are compatible with mass spec, however. The other approach would be to add a strong chaotrope, either urea or guanidine-HCl, at several molar. These are small molecules that can be removed later. You would have to experiment to find the minimum necessary concentration to keep the Zn²⁺-free protein in solution, especially if you don't want to completely unfold the protein, since refolding a completely denatured protein can be a challenge.

Dear Peyton, did you make sure that you are always comparing the same T1 excited state?

I've modeled UDFT and TDDFT excited states, both open singlet and triplet, and I've obtained quite similar results. However, changing the geometry also changes the distribution of the excited states, such that T1 may have been moved to other Tn position.

Best, Pablo

Depending on which tool you use, there are image processing packs that offer you several possibilities as far as image segmentation goes. MATLAB, C++, Python and other environments/languages allow you to customize your algorithm/program.

You will also need to work with pattern recognition techniques for clustering and classification of your subimages.

May consider using Permai fluorescence dye.

Thank you very much . really grateful to you.

Labeling plant probes with biotin is a common technique used in molecular biology research. Biotin-labeled probes are advantageous because they allow for nonradioactive detection and are safer to handle than radiolabeled probes. Here’s how you can label plant probes with biotin:

Remember that the effectiveness of your biotin-labeled probe depends on its specificity, concentration, and hybridization conditions. Proper controls and optimization are essential for successful Oligo-FISH experiments. Happy probing! 🌱🔬

For more detailed protocols, you can refer to the SpringerLink article on Nonradioactive Plant Small RNA Detection Using Biotin-Labeled Probes1.

In RevMan 5.4, which is primarily used for conducting systematic reviews and meta-analyses, you typically do not directly create forest plots within the software. Instead, forest plots are generated using statistical software packages such as R, Stata, or Review Manager, and then imported into RevMan for inclusion in your review.

Here's a general outline of how you can label forest plots in RevMan 5.4 once they have been generated:

Remember to follow any specific formatting guidelines or instructions provided by the journal or organization you are submitting your systematic review to. This ensures that your forest plots and other figures meet the required standards for publication.

In their latest publication in STAR protocols (PMID 38280198), Refaeli et al. published a detailed protocol for marking engram cells using a modified version of CLARITY in combination with a rabbit monoclonal c-Fos antibody (Synaptic Systems #226 008 [https://www.sysy.com/product/226008]).

Dear Dilan Serdar

As membrane I used nitrocellulose and I denatured the nick-translated DNA probes at 95°C for 5 minutes before spotting them on the nitrocellulose, then I baked it for about 1 hr at 80°C in a drying oven. Hope this helps.

Hello Ethan,

As per the information provided by you, there could be two possiblities.

1. You may have wrongly counted the cells while plating, or

2. You may have wrongly concluded the confluency state of cells.

I would infer that 15cm dish with 5e6 seeding density may have become overconfluent on Monday because of which there is lack of space for cells to grow further as well as the media may have been depleted of nutrients.

On the other hand, the 15cm dish with 3e6 seeding density may have almost reached confluency with some space left for cells to grow besides the media still being fresh and nutrient- rich.

I would suggest you plate less cells this thursday (say 3e6 so that it reaches 80-90% confluency after 4 days i.e., Monday/Tuesday and 1.5e6 cells so that it reaches near confluency at one/two days later i.e., Wednesday/Thursday) with the SAME amount of media in both the 15cm dishes.

Best.

Have you found a solution already?

Hi Talia,

I have looked into this particular question in the Methods paper I have published ; please see the link below. I did not use Proteome discoverer - I used MaxQuant and configured each of the TMT channel modification as a variable modification so that I could search my mass spec file to see how many peptides were not labeled.

Let me know if you can access the paper link below, if not I can also just email it to you.

All the best,

Hediye.

Yes.

In the realm of machine learning, the main distinction between supervised and unsupervised learning lies in the nature of the dataset used for training.

Supervised Learning Dataset:

In supervised learning, the dataset consists of labeled examples, where each data instance is associated with a corresponding target or output value. The dataset includes both input features and the desired output or target variable. The aim of supervised learning is to learn a mapping function that can accurately predict the target variable based on the input features. The model is trained using labeled examples, allowing it to generalize and make predictions on unseen data. Common examples of supervised learning algorithms include linear regression, decision trees, and support vector machines.

Unsupervised Learning Dataset:

On the other hand, unsupervised learning involves unlabeled datasets, meaning they do not have corresponding target values. In this scenario, the model learns patterns, structures, or relationships within the data based solely on the input features. The objective of unsupervised learning is to discover inherent patterns or groupings within the data without prior knowledge of the desired output. Common unsupervised learning algorithms include clustering algorithms such as k-means clustering and dimensionality reduction techniques like principal component analysis (PCA).

I think it would be OK to compare the fluorescence intensities before and after treatment of the same type of cell, but it would be more difficult to compare different cell types because the cell types could differ in important parameters such as size, volume, and internal composition.

Is the mean intensity of a fluorescent signal a reasonable measure for relative quantification? Yes, but you should also show the distributions, since a heterogeneous distribution could lead to misleading results if only the mean were presented.

Dear Jebari Chaker ,

Classification and clustering are two methods of pattern identification used in machine learning. Although both techniques have certain similarities, the difference lies in the fact that classification uses predefined classes in which objects are assigned, while clustering identifies similarities between objects, which it groups according to those characteristics in common and which differentiate them from other groups of objects. These groups are known as "clusters".

Regards,

Shafagat

G.Dharmamoorthy First of all, I really appreciate your help. About changing buffer. I already balance the column with sumo cleavage buffer before adding the sumo protease. Unfortunately is still not working.

If it is labeled as 1000X, it is intended to be added at 1 part per 1000 by volume. There is no way to convert to molarity unless the concentration in molarity or g/L is stated on the package or in the product information.

The music industry employs a variety of marketing techniques to promote artists and their music. Social media plays a pivotal role, enabling artists to connect with their fans directly and share content. Influencer collaborations, where musicians partner with popular online personalities, amplify their reach. Streaming platforms offer personalized playlists, and artists can utilize data-driven insights for targeted advertising. Music videos on YouTube and Vevo create visual engagement. Live performances and tours not only generate revenue but also build a loyal fan base. Partnerships with brands and sync licensing in commercials or movies broaden exposure. Lastly, viral challenges and trends on platforms like TikTok can propel a song to stardom.

Just saw that I wrote RdTomato, i meant TdTomato of course

Assuming that the labeled glucose powder is not sterile, you have 3 options.

1. Prepare a concentrated stock solution of the labeled glucose and pass it through a sterile syringe filter into a sterile container.

2. Prepare the medium from powder, including the labeled glucose powder, and then sterilize it in the usual way.

3. Add the non-sterile labeled glucose as powder or stock solution directly to sterile medium, then re-sterilize the medium by membrane filtration.

Try holding ALT key and type 0179

PBS is a a reasonable choice because it lacks amino groups that could react with the dye. However, it is usually recommended to use fresh 0.1 M sodium bicarbonate which, being a bit more basic than PBS, increases the rate of the labeling reaction.

Isabell Hummel The error message you've encountered in PopART, which reads "Error parsing sequence data; Tree labels do not match alignment labels," indicates a mismatch between the labels in the tree block and the labels in the alignment block within your NEXUS file. Despite your efforts to verify the labels, it's important to consider additional factors that might be contributing to this error:

1. Case Sensitivity: Confirm that the labels in both the tree block and alignment block match in terms of capitalization. NEXUS format is case-sensitive, so even minor differences in capitalization can cause this error.

2. Label Consistency: Double-check for any labels in the tree block that may be missing in the alignment block or vice versa. Even a single missing or additional label can lead to this error.

3. Whitespace Characters: While you mentioned verifying tabs and spaces, it's advisable to also inspect for any non-visible whitespace characters, particularly at the end of lines or within the labels, as these can sometimes be the source of the issue.

4. Special Characters: Ensure that the labels do not contain any special characters or symbols that might not be recognized correctly by the software.

5. Tree Format: Validate that the tree block adheres to the correct formatting specified by the NEXUS format standards. Ensure that it starts with "TREE" and that the labels are enclosed correctly.

6. Label Length: Some software may impose limits on label length. Examine your labels to ensure they are not excessively long, which could exceed character limits in PopART.

If you've meticulously reviewed these aspects and the error persists, consider recreating the tree block or alignment block from scratch. Occasionally, unnoticed errors can slip into the file during editing. Additionally, verify that you are using the most up-to-date version of PopART, as software updates may address compatibility issues.

It seems your problem is to find isomorphic subgraphs. The search can be accelerated by assigning statistical signatures to the vertices and edges. An example assign the degree of a vertex and the sum of degrees of this vertex's direct neighbors.

Maybe of interest

Regards,

Joachim

Dear Abdulkader Helwan ,

Zero-shot object detection (ZSD) is the task of object detection where no labeled training data is available for some target object classes. For example, there are times we'd like to detect objects that are hard to find in the class list of pre-trained models.

https://www.clarifai.com/blog/zero-shot-object-detection#:~:text=Zero%2Dshot%20object%20detection%20(ZSD,list%20of%20pre%2Dtrained%20models.

Regards,

Shafagat

Thanks

1 very rarely or not applies to me

2 rarely applies to me

3 sometimes applies to me

4 often applies to me

5 very often applies to me

It was easier for me to translate from German

see also

https://surveyyourself.files.wordpress.com/2018/01/irrational-procrastination-scale.pdf as noted in

This is one of the best resources I have ever seen on the subject:

Binyamin Kerman very kind for your answer, thanks. Exploring all your suggestions!

N

I hope/guess you know, which DNA ladder from which company you used. So simply look at the product specifications on the website or contact the provider. If you don't know, which ladder you used there is no way to properly label the size of your products and the marker. Next time document all experimental details in your lab-book including the marker specifications.

hematopoietic tissues using . please follow science direct bone marrow topics