Science method

LC-MS - Science method

Liquid chromatography–mass spectrometry (LC-MS, or alternatively HPLC-MS) is an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography (or HPLC) with the mass analysis capabilities of mass spectrometry. LC-MS is a powerful technique used for many applications which has very high sensitivity and selectivity.
Filters
All publications are displayed by default. Use this filter to view only publications with full-texts.
Publications related to LC-MS (10,000)
Sorted by most recent
Article
Full-text available
ABSTRACT Aflatoxin contamination in food commodities is a major food safety concern all over the world. These aflatoxins are a group of carcinogenic secondary metabolites produced by some fungi belonging to Aspergillus section Flavi. To minimize the level of aflatoxins in food commodities, a wide range of preand post-harvest procedures have been em...
Article
Full-text available
Seaweed surface provides a suitable substratum for the settlement of microorganisms (bacteria, viruses, and plankton). These microbial partners may have either beneficial or detrimental effects on the host since surface microbiota can act either as a protective layer to the macroalgae (symbiotic beneficial relations) or, under changing environmenta...
Article
Full-text available
Dexamethasone sodium phosphate (Dex-SP) is the most commonly used drug administered via intratympanic injection for the treatment of acute hearing loss, but its penetration efficiency into the inner ear is very low. To address this problem, we evaluated the possibility of administering dexamethasone nanosuspensions via intratympanic injection becau...
Article
Full-text available
Epidermal growth factor receptor (EGFR) is the primary target for the treatment of colorectal cancer, the third most diagnosed cancer worldwide. In recent years, regulatory changes have facilitated the approval of biosimilars aimed to bring more access to biologics to patients. However, it has also expended the requirements of non-clinical characte...
Research Proposal
Full-text available
Dear Colleagues, With the development of a globalized food market, food fraud has become a crucial issue over the world. Adulteration, substitution, and the deliberately incorrect labeling of species and geographical origins of food are considered the main cases of food fraud. Some unscrupulous traders replace special local products with inferior...
Article
Full-text available
Organophosphates (OPs) are hazardous pesticides, but an indispensable part of modern agriculture; collaterally contaminating agricultural soil and surrounding water. They have raised serious food safety and environmental toxicity that adversely affect the terrestrial and aquatic ecosystems and therefore, it become essential to develop a rapid biore...
Article
Full-text available
The Antarctic fungus Cadophora malorum produces previously undescribed cyclic heptapeptides (cadophorin A and B ) containing an anthranilic acid residue. The planar structure of these peptides was determined by high-resolution mass spectrometry combined with extensive 1D and 2D NMR spectroscopy. The absolute configuration of the amino acids was det...
Article
Full-text available
Background Over 100 mutations in the SRD5A2 gene have been identified in subjects with 46,XY disorder of sex development (DSD). Exploration of SRD5A2 mutations and elucidation of the molecular mechanisms behind their effects should reveal the functions of the domains of the 5α-reductase 2 enzyme and identify the cause of 46,XY DSD. Previously, we r...
Article
Full-text available
Photocatalytic degradation of Methylene Blue (MB) by zinc oxide/zeolite socony mobile-5 (ZnO/ZSM-5) composites was investigated. The ZSM-5 material was synthesized from red mud by a two-step hydrothermal method to which ZnO loadings at different mass ratios were subsequently performed. Characterizations using X-ray diffraction (XRD), Fourier transf...
Article
Full-text available
A new methodology for the detection of ketamine, flunitrazepam, and diazepam in beverage samples by the dried sample spot method was developed. The method is characterized by very low LODs in all tested types of beverages (100 ng/mL for ketamine, 25 ng/mL for flunitrazepam and diazepam) and great precision at the concentration of 100 ng/mL for all...
Article
Full-text available
Background To investigate the effect of mannose on radio-sensitivity of human esophageal squamous cell carcinoma (ESCC) cell line and its possible mechanism. Methods The expression of mannose phosphate isomerase (MPI) in human esophageal cancer cell lines were detected by Western blot. The inhibitory effect of mannose on human esophageal cancer ce...
Article
Full-text available
Objectives Formalin-fixed paraffin-embedded (FFPE) tissue is the standard material for diagnostic pathology but poses relevant hurdles to accurate protein extraction due to cross-linking and chemical alterations. While numerous extraction protocols and chemicals have been described, systematic comparative analyses are limited. Various parameters we...
Article
Full-text available
Background Cerebrospinal fluid (CSF) is an important biofluid for biomarkers of neurodegenerative diseases such as Alzheimer’s disease (AD). By employing tandem mass tag (TMT) proteomics, thousands of proteins can be quantified simultaneously in large cohorts, making it a powerful tool for biomarker discovery. However, TMT proteomics in CSF is asso...
Article
Full-text available
Cocoa products have a remarkable chemical and sensory complexity. However, in contrast to other fermentation processes in the food industry, cocoa bean fermentation is left essentially uncontrolled and is devoid of standardization. Questions of food authenticity and food quality are hence particularly challenging for cocoa. Here we provide an illus...
Article
Full-text available
Background Among the most aggressive and rapidly lethal types of lung cancer, lung adenocarcinoma is the most common type. Exosomes, as a hot area, play an influential role in cancer. By using proteomics analysis, we aimed to identify potential markers of lung adenocarcinoma in serum. Methods In our study, we used the ultracentrifugation method to...
Article
Full-text available
Objectives Verifying new reagent or calibrator lots is crucial for maintaining consistent test performance. The Institute for Quality Management in Healthcare (IQMH) conducted a patterns-of-practice survey and follow-up case study to collect information on lot verification practices in Ontario. Methods The survey had 17 multiple-choice questions a...
Article
Full-text available
Different routes of access to mesembrine alkaloids uniquely produced by the medicinal succulent Sceletium tortuosum (Aizoaceae) are thus highly sought after in the natural products sector. In order to develop a biotechnological method, the establishment of in vitro microshoot and callus suspension cultures was investigated using in vitro seedlings...
Article
Full-text available
In this paper, removal of malachite green (MG) by two separate processes, namely electrochemical oxidization polymerization and electrochemical reduction precipitation process, was studied. Reaction rate constants (k⁰), exchange current density i⁰, the transfer coefficients β and the diffusion coefficient D values were all tested. TR-FTIR (time-res...
Article
Full-text available
Background The mortality rate of patients with diquat (DQ) poisoning is extremely high due to insufficient understanding of DQ-induced injury. This study aimed to summarize the characteristics of DQ poisoning as well as analyse the correlation between plasma DQ concentration and patient outcomes, thus providing a new strategy for diagnosis and trea...
Article
Full-text available
A new method was developed and validated for the simultaneous determination of nicotine and tobacco-specific nitrosamines (TSNAs) 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and N -nitrosonornicotine (NNN) in two different tests matrices: porcine buccal epithelium tissue and phosphate buffered saline (PBS) extracts of smokeless tobacco pro...
Article
Full-text available
Maternal hyperglycemia is associated with disrupted transplacental arachidonic acid (AA) supply and eicosanoid synthesis, which contribute to adverse pregnancy outcomes. Since placental inositol is lowered with increasing glycemia, and since myo-inositol appears a promising intervention for gestational diabetes, we hypothesized that myo-inositol mi...
Article
Full-text available
Either extracts, cell-free suspensions or bacterial suspensions are used to study bacterial lipid peroxidation processes. Along with gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, and several other strategies, the thiobarbituric acid test is used for the determination of malondialdehyde (MDA) as the basis for the com...
Article
Full-text available
Background Alternative treatment strategies in melanoma beyond immunotherapy and mutation-targeted therapy are urgently needed. Wild-type isocitrate dehydrogenase 1 (wtIDH1) has recently been implicated as a metabolic dependency in cancer. The enzyme protects cancer cells under metabolic stress, including nutrient limited conditions in the tumor mi...
Article
Full-text available
Antibody–drug conjugates (ADCs) are a new class of biotherapeutics, consisting of a cytotoxic payload covalently bound to an antibody by a linker. Ligand-binding assay (LBA) and liquid chromatography-mass spectrometry (LC-MS) are the favored techniques for the analysis of ADCs in biomatrices. The goal of our review is to provide current strategies...
Article
Full-text available
Background: The aim was determining bioequivalence between pantoprazole buffered powder for oral suspension and pantoprazole enteric coated tablets under fasting conditions in healthy volunteers.Methods: In randomized cross-over study, participants were administered a single oral dose of pantoprazole powder as suspension 40 mg (sodium bicarbonate a...
Article
Full-text available
The faba bean is one of the earliest domesticated crops, with both economic and environmental benefits. Like most legumes, faba beans are high in protein, and can be used to contribute to a balanced diet, or as a meat substitute. However, they also produce the anti-nutritional compounds, vicine and convicine (v-c), that when enzymatically degraded...
Article
Full-text available
Sulfoxaflor is a new systemic insecticide developed as a replacement for older neonicotinoids which are known to be toxic to pollinators. However, its metabolism in nectar and effect on nectar biosynthesis have not been investigated. After soil and foliar application, sulfoxaflor and its main metabolites in soil, leaf and Salvia splendens nectar, w...
Article
Full-text available
Glycerol can be metabolized to glucose via gluconeogenesis or lactate via glycolysis. It is unknown if glycerol is metabolized similarly in the portal and systemic circulations in humans. Eight metabolically healthy overnight-fasted individuals received equimolar amounts of 13C3-glycerol orally and intravenously on two separate occasions with seria...
Article
Full-text available
Background The COVID-19 pandemic is likely to represent an ongoing global health issue given the potential for new variants, vaccine escape and the low likelihood of eliminating all reservoirs of the disease. Whilst diagnostic testing has progressed at a fast pace, the metabolic drivers of outcomes–and whether markers can be found in different biof...
Article
Full-text available
Kae1 is a subunit of the highly evolutionarily conserved KEOPS/EKC complex, which is involved in universal (t6A37) tRNA modification. Several reports have discussed the participation of this complex in transcription regulation in yeast and human cells, including our previous observations of KaeA, an Aspergillus nidulans homologue of Kae1p. The aim...
Preprint
Full-text available
Breast cancer is a heterogeneous disease, and the survival rate of patients with breast cancer strongly depends on their stage and clinicopathological features. Chemoradiation therapy is commonly employed to improve the survival of patients with advanced breast cancer. However, the treatment process is often accompanied by the development of drug r...
Article
Full-text available
Previous and recent literature acquisitions suggested that cultured calli are an efficient and meaningful model to investigate the extent and fate of prenylation of phenylpropanoid cores in plants belonging to the Rutaceae and Apiaceae families upon administration of putative biosynthetic precursors. To this concern, in the present manuscript, we i...
Article
Full-text available
This study was designed to evaluate the chemical fingerprints and the antioxidant, anti-inflammatory and hypolipidemic activity of selected Crepis species collected in Greece, namely, C. commutata, C. dioscoridis, C. foetida, C. heldreichiana, C. incana, C. rubra, and Phitosia crocifolia (formerly known as Crepis crocifolia). For the phytochemical...
Article
Full-text available
Poster session 1, September 21, 2022, 12:30 PM - 1:30 PM Alexander Fleming's discovery of the antibiotic ‘Penicillin’ and many more has inspired after saving millions of lives and spared immeasurable suffering worldwide. However, he also warned about the consequences of abuse of antibiotics that could lead to the expiry of the golden age of antibio...
Article
Full-text available
Background Cancer-associated fibroblasts (CAFs) are major components of the tumor microenvironment (TME). Hypoxic TME is known to promote tumor progression. However, how a hypoxic condition regulates CAFs remains elusive.Methods To investigate the underlying mechanism involved in the regulation of gastric cancer (GC) progression by hypoxic CAFs, we...
Article
Full-text available
Neuroinflammation plays a critical role in the pathogenesis of most neurological and neurodegenerative diseases and therefore represents a potential therapeutic target. In this regard, accelerating the resolution process in chronic neuroinflammation may be an effective strategy to deal with the cognitive consequences of neuropathology and generaliz...
Article
Full-text available
Macrophages synthesize active vitamin D (1,25-dihydroxy-vitamin D) and express the vitamin D receptor in the nucleus; however, vitamin D metabolism in relation to macrophage polarization and function is not well understood. We studied monocyte-derived macrophages (MDMs) from human buffy coats polarized into M0, M1 (LPS + IFNγ), M2a (IL4 + IL13) and...
Article
Full-text available
RiPPs (ribosomally-synthesized and post-translationally modified peptides) are a class of pharmaceutically-relevant natural products expressed as precursor peptides before being enzymatically processed into their final functional forms. Bioinformatic methods have illuminated hundreds of thousands of RiPP enzymes in sequence databases and the number...
Article
Full-text available
Enterococcus species are Gram-positive bacteria that are normal gastrointestinal tract inhabitants that play a beneficial role in the dairy and meat industry. However, Enterococcus species are also the causative agents of health care-associated infections that can be found in dairy and fermented food products. Enterococcal infections are led by str...
Article
Full-text available
In this work, we propose a general methodology to assess the bioactive potential (BP) of extracts in the quest of vegetable-based drugs. To exemplify the method, we studied the anticancer potential (AP) of four endemic species of genus Hypericum (Hypericum canariense L, Hypericum glandulosum Aiton, Hypericum grandifolium Choisy and Hypericum reflex...
Article
Full-text available
Immunoprecipitation (IP) of endogenously expressed proteins is one of the most biologically relevant techniques to identify protein-protein interactions. We describe an adaptable IP protocol reliant on a specific antibody to the target protein. We detail a quantitative proteomics workflow for the unbiased identification of co-immunoprecipitating pr...
Article
Full-text available
Schistosomiasis control remains a public health concern, and there is a need to evaluate new strategies for targeting larval and adult stages of the parasite. As Pedilanthus tithymaloides is empirically used to treat schistosomiasis, it becomes essential to know its effective action scientifically. This study assessed the cercaricidal and schistoso...
Preprint
Full-text available
Although tacrolimus has been widely used in patients undergoing lung transplantation, few studies have reported the pharmacokinetics of tacrolimus in Chinese patients after lung transplantation. Thus, we aimed to investigate its pharmacokinetics and influential factors in this patient cohort in the early stage after lung transplantation. We enrolle...
Poster
Full-text available
Das durchschnittliche Atemvolumen eines Menschen beträgt 7,5 Liter pro Minute, also rund 10 m3 pro Tag, weshalb es wichtig ist, dass die regelmäßig eingeatmete Raumluft frei von gesundheitsschädlichen Stoffen ist. Neben allgemein bekannten Problemstoffen wie Feinstaub, Asbest, PCBs und ähnlichem sind inzwischen auch Mykotoxine in den Blickwinkel ge...
Article
Full-text available
Motivation Compound structure identification is using increasingly more sophisticated computational tools, among which machine learning tools are a recent addition that quickly gains in importance. These tools, of which the method titled Compound Structure Identification:Input Output Kernel Regression (CSI:IOKR) is an excellent example, have been u...
Article
Full-text available
We report the synthesis of novel [4-(3-amino-4-mehoxy-5-methyl phenyl)-1-oxo-1H-phthalaz-2-yl] acetic acid hydrazide (APPH), followed by its characterization using X-ray diffraction (XRD), Fourier transforms infrared (FT-IR) spectroscopy, 1 H-NMR spectroscopy, and LC/MS. Further, the inhibition effect of the varying concentration of APPH on the cor...
Article
Full-text available
Introduction Chestnut rot caused by the fungus Gnomoniopsis smithogilvyi is a disease present in the world’s major chestnut growing regions. The disease is considered a significant threat to the global production of nuts from the sweet chestnut (Castanea sativa). Conventional fungicides provide some control, but little is known about the potential...
Article
Full-text available
In this work, a novel Z-scheme heterojunction of ZIF67/WO3 nanocomposite is successfully prepared via immobilizing WO3 on the MOFs ZIF67 and confirmed by measuring XRD, FT-IR spectroscopy, XPS, SEM, TEM, and EDS. UV–vis and Mott-Schottky measurements. The photocatalytic activity of the as-prepared ZIF67/WO3 is evaluated by degrading methylene blue...
Article
Full-text available
Yaks (Bos mutus) live in the Qinghai–Tibet plateau. The quality of yak meat is unique due to its genetic and physiological characteristics. Identification of the proteome of yak muscle could help to reveal its meat-quality properties. The common proteome, phosphoproteome, and N-glycoproteome of yak longissimus thoracis (YLT) were analyzed by liquid...
Article
Full-text available
The origins of preexisting SARS-CoV-2 cross-reactive antibodies and their potential impacts on vaccine efficacy have not been fully clarified. In this study, we demonstrated that S2 was the prevailing target of the preexisting S protein cross-reactive antibodies in both healthy human and SPF mice. A dominant antibody epitope was identified on the c...
Preprint
Full-text available
Alterations in cellular metabolism represent an important response to proliferative signals in both normal and transformed tissues. The benign proliferative process of liver regeneration after partial hepatectomy offers insight into homeostatic mechanisms to control liver mass, which are disrupted in liver disease induced by viral factors, alcohol,...
Article
Full-text available
Peanuts and peanut butter play an important role nutritionally in improving the diets of individuals in many parts of Africa, especially in the fight against child malnutrition. However, in developing countries such as Zimbabwe, most of the raw peanuts and peanut butter produced in backyard industries are sold in informal markets and rarely undergo...
Article
Full-text available
Lavandula angustifolia is the most widely cultivated Lavandula species for medicinal use. In this study, chemical and biological evaluation of L. angustifolia aqueous, methanol (MeOH), ethanol (EtOH), ethyl acetate (EtOAc), and chloroform (CHCl3) extracts were conducted. Phytochemically, the extracts’ total phenol and flavonoid contents and their a...
Article
Full-text available
Being born small or large for gestational age (SGA and LGA, respectively), combined with suboptimal early postnatal outcomes, can entail future metabolic alterations. The exact mechanisms underlying such risks are not fully understood. Lipids are a highly diverse class of molecules that perform multiple structural and metabolic functions. Dysregula...
Article
Full-text available
Guggulu (Commiphora wightii oleo-gum resin) is exported in more than 42 countries including developed countries like the United Kingdom and the United States of America. The demand for guggulu is more than its production. Various purification processes have been mentioned in Ayurvedic classical literature which are not explored well yet, so it is i...