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LC-MS/MS - Science method

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Publications related to LC-MS/MS (10,000)
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Article
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Tendinopathy is a common musculoskeletal disorder which results in chronic pain and reduced performance. The therapeutic effect of stem cell derived-small extracellular vesicles (sEVs) for tendinopathy has been validated in recent years. However, whether large extracellular vesicles (lEVs), another subset of extracellular vesicles, possesses the ab...
Article
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Carotenoids are important color pigments that contribute to human health. Haskap (Lonicera caerulea subsp. edulis), a shrub mainly distributed in Hokkaido, produces violet-blue berries low in carotenoid amount. We have conducted interspecific hybridization between Miyama-uguisukagura (Lonicera gracilipes var. glandulosa) and haskap to obtain inters...
Article
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Bu araştırmanın amacı, Abelmoschus esculentus’a ait çiçek kısımınlarının etanolik ekstraktının antioksidan ve antikolinerjik özelliklerini, fenolik bileşik profilini ve antibakteriyel aktivitesini araştırmaktır. Fenolik bileşiklerin analizi LC-MS/MS ile gerçekleştirilmiştir. Antioksidan kapasitesi (radikal giderme, metal indirgeme gücü ve toplam an...
Article
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Background Treatment for glaucoma has traditionally been limited to reducing intraocular pressure (IOP). Inhibiting oxidative stress in the trabecular meshwork (TM) is regarded as a new treatment for glaucoma; however, the effects do not meet expectations. Exploring the mechanism by which oxidative stress and antioxidant stress occur in TM cells wi...
Article
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To obtain immunomodulatory peptides from isolated soy protein (ISP), pepsin was selected to prepare hydrolysates and 4-h treatment (Pepsin-ISPH4h) showed the highest yield and immunomodulatory activities. The Pepsin-ISPH4h was sequentially fractionated by 30, 10 and 1-kDa molecular weight cut-off (MWCO) membranes, in which 1-kDa MWCO permeate (1P)...
Article
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Objective Bile and its individual components, mainly bile acids, are important for digestion and drive bacterial community dynamics in the upper gastrointestinal tract of chickens. However, specific responses to bile acids have been characterized in only a few commensal bacteria, and it is unclear how other members of the microbiota respond to bili...
Article
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Objective We developed an assay to measure the concentration of 25 hydroxyvitamin D 2 and D 3 in protein extracts derived from stored neonatal dried blood spots. During this study, we postulated that these samples had been contaminated with exogenous vitamin D metabolites because of the addition of bovine serum albumin (BSA) as part of an extractio...
Article
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Background Dabie bandavirus, also termed as severe fever with thrombocytopenia syndrome virus (SFTSV), was first isolated in China in 2010. At this time, the virus was found to have spread to South Korea, Japan, and other countries. A high case fatality rate is reported for SFTS, ranging from 12–50% within various sources. Several omics for clinica...
Article
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Background Aerobic metabolism generates reactive oxygen species that may cause critical harm to the cell. The aim of this study is the characterization of the stress responses in the model aromatic-degrading bacterium Paraburkholderia xenovorans LB400 to the oxidizing agents paraquat and H 2 O 2 . Methods Antioxidant genes were identified by bioin...
Article
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Background Glyphosate is the declared active component of the most extensively used herbicides in the world, and is therefore widely present in the environment. Glyphosate urinary levels represent a relevant biomarker for each individual’s exposure to glyphosate-based herbicides. However, GLY urine level measurement is controversial because differe...
Article
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Background The legalization of hemp in the USA has led to tremendous growth in the availability of hemp-derived products, particularly cannabidiol (CBD) products. The lack of regulatory oversight in this industry has resulted in the marketing and sale of CBD products with questionable ingredients and quality. The aim of the current study was to exa...
Article
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Background Multiple sclerosis (MS) is an autoimmune, neurodegenerative disorder with a strong genetic component that acts in a complex interaction with environmental factors for disease development. CD4 ⁺ T cells are pivotal players in MS pathogenesis, where peripherally activated T cells migrate to the central nervous system leading to demyelinati...
Article
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Th9 cells have been shown to play crucial roles in anti-parasite immunity, pathogenic microbe infection, and allergy. Previous studies have demonstrated that Haemonchus contortus excretory and secretory proteins (HcESPs) induce the proliferation of Th9 cells and alter the transcriptional level of IL-9 as well as its related pathways in the Th9 immu...
Article
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Stanniocalcin-1 (STC1) is a hypocalcemic hormone originally identified in bony fishes. The mammalian homolog is found to be involved in inflammation and carcinogenesis, among other physiological functions. In this study, we used the TriCEPS-based ligand–receptor methodology to identify the putative binding proteins of human STC1 (hSTC1) in the huma...
Article
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Aldosterone is a steroid hormone secreted from the adrenal cortex and metabolized primarily in the kidneys. It promotes sodium retention and potassium excretion. Most plasma aldosterone exists in free form, with a rapid turnover rate. The increased in aldosterone in the body may lead to various metabolic diseases, such as primary aldosteronism, dia...
Conference Paper
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Introducción. El uso continuo de herbicidas, frecuentemente con múltiples aplicaciones durante el mismo ciclo agrícola, provoca el surgimiento de biotipos/poblaciones de malezas resistentes. La resistencia es la capacidad hereditaria de algunos individuos para crecer y reproducirse sexualmente después del tratamiento de un herbicida a dosis de camp...
Article
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1,2-Unsaturated pyrrolizidine alkaloids (PA), their corresponding N -oxides (PANO), and tropane alkaloids (TA) are toxic secondary plant metabolites. Their possible transfer into the milk of dairy cows has been studied in feeding trials; however, only few data on the occurrence of these toxins in milk are available. In this study, the development o...
Article
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Since the publication of the inspection guide on cleaning validation in 1993 at the FDA, several studies have been published to demonstrate the suitability of TOC in measuring contaminating residues. 1-7 The use of TOC can be justified for direct tests of surface samples, as well as for tests of indirect samples (rinse water). In either case, since...
Article
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Background The mortality rate of patients with diquat (DQ) poisoning is extremely high due to insufficient understanding of DQ-induced injury. This study aimed to summarize the characteristics of DQ poisoning as well as analyse the correlation between plasma DQ concentration and patient outcomes, thus providing a new strategy for diagnosis and trea...
Article
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Impaired inhibitory signaling underlies the pathophysiology of many neuropsychiatric and neurodevelopmental disorders including autism spectrum disorders and epilepsy. Neuronal inhibition is regulated by synaptic and extrasynaptic γ-aminobutyric acid type A receptors (GABAARs), which mediate phasic and tonic inhibition, respectively. These two GABA...
Article
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Plants belonging to the genus Hypericum L. are widely used in traditional folk medicine due to their hypericin and pseudohypericin contents. In this study, we investigated the biological activity, phenolic and elemental content of methanol and acetone extracts of sheepskin grass, Hypericum heterophyllum Vent. a species that that lacks both hyperici...
Article
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4,4′-Sulfonyl-diphenol (BPS), as a widespread environmental hormone-like micropollutant, is difficult to be degraded in the environment. In this study, the removal of BPS with multi-enzymes extracted from waste sludge and reed sediment was studied at 298 K, 310 K, and 328 K. Results show that BPS could be removed efficiently and was time–temperatur...
Article
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Idiopathic Granulomatous Mastitis (IGM) is a disease that clinically mimics breast cancers with symptoms of pain, edema, erythema, nipple discharge, nipple retraction, and fistula. Although IGM is considered to be formed by autoimmune responses or infections, the molecular mechanism behind formation and progress is unknown. Therefore, in this study...
Article
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Bovine paratuberculosis is a serious chronic disease of the gastrointestinal tract that causes economic losses and dramatically affects animal health in livestock. The underlying infectious agent, Mycobacterium avium subspecies paratuberculosis (MAP), cannot reliably be detected by standard diagnostic tests due to the long asymptomatic disease stag...
Article
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Background Medulloblastoma (MB) is the most common pediatric brain tumor. Although standard of care treatment generally results in good prognosis, many patients exhibit treatment-associated lifelong disabilities. This outcome could be improved by employing therapies targeting the molecular drivers of this cancer. Attempts to do so in the SONIC HEDG...
Article
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Lupinus is a wide genus, comprising between 300 and 500 species, most of them represented in America. Mexico is a secondary distribution center with more than 100 species growing along the highlands. Due to morphological similarities, the taxonomy of wild Lupinus species is still incomplete. It is, therefore, useful to collect morphological, chemic...
Article
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The aim of this article is to provide a unified analytical methodology that allows the determination of 181 pesticides in flowers, leaves and tree trunks, in compliance with the current legislation in Europe. Most of these commodities are not included in the regulation and do not have established a maximum residue limit as many of them are not edib...
Article
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Nifuroxazide (NAZ), a nitrofuran derivative used to treat diarrhea, has been recently shown to possess anticancer activity. However, its pharmacokinetic profile is poorly known. The pharmacokinetic profile of NAZ was thus investigated in mice using a newly developed method based on high-performance liquid chromatography–tandem mass spectrometry (HP...
Article
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The phosphocholine and phospholipid that is hexadecyl monoester of phosphocholine (alkyl phospholipid) is a miltefosine drug used to treat all types of leishmaniasis. It is a broad-spectrum antimicrobial, anti-leishmanial, anti-cancer phospholipid drug. It is used for the treatment of breast cancer and cutaneous metastasis. This present study of mi...
Article
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Paenibacillus sp. GKG was isolated based on its ability to produce hydrolysis zones on agar plates containing yeast cell wall substrate as the single carbon source. The extracellular enzymes secreted into the culture medium were identified by LC-MS/MS proteomics. Endo-β-1,3-glucanase PsLam81A containing GH81 catalytic and the CBM56 carbohydrate-bin...
Article
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This study aimed to evaluate the pharmacokinetics of acipimox in rats under simulated high altitude hypoxia conditions. A sensitive and reliable LC–MS/MS method has been established for the quantitation of acipimox in rat plasma and tissue homogenate and validated according to the guidelines of the European Medicines Agency (EMA) and the US Food an...
Article
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Bioinformatic tools are widely used in predicting potent bioactive peptides from food derived materials. This study was focused on utilizing sea cucumber processing by-products for generating antioxidant and ACE inhibitory peptides by application of a range of in silico techniques. Identified peptides using LC−MS/MS were virtually screened by PepRa...
Article
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In this study, we investigated the cross-talk between mGlu1 and CB1 receptors in modulating GABA hippocampal output in whole-cell voltage clamp recordings in rat hippocampal acute slices, in organotypic hippocampal slices exposed to oxygen and glucose deprivation (OGD) and in gerbils subjected to global ischemia. CB1 receptor expression was studied...
Article
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An analytical method simultaneously detecting pesticide and its metabolites, especially with higher toxicity, was urgently needed for supervision and safety evaluation of agricultural products. In the present study, a modified QuEChERS method coupled with a ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for simul...
Conference Paper
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Background Recent guidelines for Systemic Lupus Erythematosus (SLE) recommend using a hydroxychloroquine (HCQ) dose less than 5.0 mg/kg/day to reduce the risk of HCQ-induced retinopathy. The aims of our study were to (1) evaluate if HCQ reduction dose to 5 mg/kg/day is associated with increased risk of SLE flares, (2) compare HCQ blood levels betwe...
Article
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A new method was developed for the identification and determination of L-ergothioneine in cosmetics based on ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The pretreatment method, chromatographic column, chromatographic conditions, and mass spectrometric conditions of cosmetic samples were optimized. Methanol was cho...
Preprint
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Liquid chromatography coupled with bottom up mass spectrometry (LC-MS/MS)-based proteomics is increasingly used to detect changes in post-translational modifications (PTMs) in samples from different conditions. Analysis of data from such experiments faces numerous statistical challenges. These include the low abundance of modified proteoforms, the...
Article
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Methoxyfenozide and pymetrozine are used for pest control in the cultivation of Chinese cabbage. This has raised concerns in recent years due to health risks. Therefore, this study aimed to determine the residual concentrations of pesticides in the target crop and associated health risks. The dynamics and influence of environmental factors on the d...
Preprint
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Background: The presence of lymph node metastasis (LNM) affects treatment strategy decisions in T1NxM0 colorectal cancer (CRC), but the currently used clinicopathological-based risk stratification cannot predict LNM accurately. In this study, we established a classifier for predicting LNM in T1 CRC. Methods: We detected proteins in formalin-fixed p...
Article
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Sulfoxaflor is a new systemic insecticide developed as a replacement for older neonicotinoids which are known to be toxic to pollinators. However, its metabolism in nectar and effect on nectar biosynthesis have not been investigated. After soil and foliar application, sulfoxaflor and its main metabolites in soil, leaf and Salvia splendens nectar, w...
Article
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(1) Background: Disturbances in the sphingolipid profile are observed in many diseases. There are currently no data available on the evaluation of sphingolipids and ceramides in cholelithiasis in children. The aim of this study was to evaluate the concentrations of sphingolipids in the sera of pediatric patients with gallstones. We determined their...
Article
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Antibiotics, especially fluoroquinolones, have been exhaustively used in animal husbandry. However, very limited information on the occurrence and exposure assessment of fluoroquinolone residues in chicken and pork in China is available to date. Thus, a total of 1754 chicken samples and 1712 pork samples were collected from 25 provinces in China an...
Article
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Characterization of perfluorohexane sulfonate (PFHxS) isomers, a chemical proposed for listing under the Stockholm Convention, is important to elucidate its environmental behaviors and sources. Optimized chromatographic separation coupled with monitoring of the characteristic fragments enabled the identification of four mono-substituted and two di-...
Article
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Kratom (Mitragyna speciosa (Korth.) Havil.) has been used to reduce blood sugar and lipid profiles in traditional medicine, and mitragynine is a major constituent in kratom leaves. Previous data on the blood sugar and lipid-altering effects of kratom are limited. In this study, phytochemical analyses of mitragynine, 7-hydroxymitragynine, quercetin,...
Article
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Workplace culture has been studied for impact on health risk; however, connections with robust biologic markers of health remain to be established. We examined associations between the work environment and urinary levels of catecholamines and their metabolites as biomarkers of sympathetic nervous system activity, indicative of stress. We recruited...
Article
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N -Glycan alterations contribute to the pathophysiology and progression of various diseases. However, the involvement of N -glycans in knee osteoarthritis (KOA) progression at the tissue level, especially within articular cartilage, is still poorly understood. Thus, the aim of this study was to spatially map and identify KOA-specific N -glycans fro...
Article
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Background: Although metabolomic analysis for patients with non-alcoholic fatty liver disease (NAFLD) was a promising approach to identify novel biomarkers as targets for the diagnosis of NAFLD, the serum metabolomics profile of early-stage NAFLD patients from central China remain unknown. Objective: The aim of this study was to explore the meta...
Article
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Background: Methylmalonic acid (MMA) is an essential indicator of vitamin B12 (VB12) deficiency and inherited metabolic disorders (IMDs). The increasing number of requests for MMA testing call for higher requirements for convenient MMA testing methods. This study aims to develop a convenient quantification method for serum MMA. Methods: The method...
Conference Paper
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Melatonin (MEL) is associated with the formation of incremental lines and calcification of dentin (Mishima et al., 2018). However, the effects of MEL intake during osteogenesis on bone formation in rats have not been clarified. The purpose of this study is to investigate the effect of MEL intake in lactating rats on the morphology and quality of la...
Article
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Ochratoxin A (OTA) is a mycotoxin produced by several species of Aspergillus and Penicillium and commonly detected in a wide range of foodstuffs. The purpose of this work was to monitor the presence of OTA in cheeses and pork meat products. A simple and accurate “dilute and shoot” method with no need of immunoaffinity column and isotopic labeled in...
Article
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Pufferfish is nutritious and delicious, but the tetrodotoxin (TTX) that may exist in its body poses a serious safety hazard. It is important to use scientific and effective methods to detect the TTX in pufferfish, but most of the existing methods require complex pre-treatment steps and have sample lethality. The solid-phase microextraction (SPME) t...
Preprint
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Extracellular vesicles (EVs) of nanoscale carry the biological information of parent cells, however, there is no consensus on the culture, collection, and purification of cell-derived EVs. We optimized the concentration of extracelluar vesicle (EV)-depleted serum (EDS), time of collection, and the extraction method for small EVs (sEVs) derived from...
Article
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The primary objective of this study was to develop and validate an efficient and accurate ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) approach as a means to detect tropifexor plasma concentrations in beagle dogs and to study its pharmacokinetic profile in beagle dogs. The chromatographic separation of tropifexor an...