Questions related to Insect
I am seeking clarification on the differences between the two commercial baculovirus systems for insect expression: Bac-to-Bac system (Invitrogen)and the MultiBac system (from by Imre Berger and colleagues) for insect expression.
It has come to my attention that specific plasmids, such as pACEBac1/pFBDM for MultiBac and pFastBac/pFastBacDual for Bac-to-Bac, are associated exclusively with their respective systems. Would someone be able to confirm whether these plasmids are indeed non-interchangeable between the two systems and, if so, an explanation for this distinction. The plasmids look similar, so is there a difference in the mechanism?
Hello, I have encountered a lot of these unusual structures while looking for Middle Triassic conodonts. The sample was dissolved in formic acid and has yielded conodonts, agglutinated foraminifera and fish teeth.
Could these perhaps be pieces of a sponge skeleton, or are they more likely pieces of plastic foam or something similar? Fused sponge skeletons seem to have more rounded edges, an these seem to have pronounced angles. Sponges also seem to rarely form pentagonal meshes...
I washed the sample pieces before dissolving them, but I wouldn't be surprised something got stuck in the cracks and nooks (e.g., there was a very small insect leg part in one of the fractions). I tested if these structures sink in water; they do, but very slowly. I am aware that some plastic does, however, sink in water.
Could anyone more experienced with micropaleontology help?
I am trying to extract DNA from a single insect from different sites. After extraction nanodrop readings show around 12-15 nanogram per microlitre concentration which is normal for the insect. However PCR amplification from two specific sites are failing everytime inspite of DNA being present. I have checked for presence of inhibitors by extracting DNA from four sites simultaneously which included the two sites mentioned above. DNA concentration is sufficient. However PCR amplification failed again. What could be the reasons for the failing PCRs?
I am attaching the gel image of the samples.
I recently encounter a devastating insect. I want to know more to control it. Any one familiar with the insect pest?
Greetings ResearchGate Community,
I was wondering if someone could kindly shed some light on the identity of the red insect in this photo? This appears to be a species of katydid but I have never seen anything like it before. Any information will be greatly appreciated.
Thank you all so much!
Can we alter the genome of a polyphagous plant insect pest to a monophagous and phytophagous insect to insectivorus? Is there any example? Please share your thoughts..
What are the methodologies involved in sequencing and aligning the genome of an unknown species of insect for the purpose of constructing a phylogenetic tree? Are there any institutions or facilities that offer sequencing services specifically tailored for unknown insect species? What are the recommended protocols in order to initiate the sequencing process?
I have been trying to compare the efficiency, cost-effectiveness, and convenience of the Piggybac and Bacmid systems, both of which have been utilized extensively in insect genetics. However, I have not been able to find a conclusive answer. If someone has worked extensively in this area, I am hoping to have some insights that could help me understand the pros and cons of each system better.
i am starting working with insect cell protein expression from previously working with bacterial protein expression. for bacteria you can pellet and then freeze the cells after expression (and sometimes it even helps the lysis later).
I am gonna express a protein via Baculovirus in insect cells can i harvest and freeze the pellet before protein extraction and purification?
It's known that phytates are a P storage in plants, in particular seeds. Plants produce enzymes to release P upon need. Also ruminants can digest phytates thanks to their gut microbiome. However, for many animals phytic acid is an antinutrient because it binds nutrients in cationic form (Ca2+, Mg2+ etc.). Insect have proven able to digest a wide variety of substrates thanks to their gut microbiome. I found mentions of phytates in insect-based products, but it wasn't addressed whether insects can digest them. Do you know if any experiment proved they can digest phytates?
This insect is an interesting major pest on tomatoes, peppers, beans, peas, corn and soybeans. During my research travels, I found it as a common insect in variegated geographical regions worldwide. Florida in USA and Debrecen in central Europe regions are two main different areas from geographical, and ecological standpoints where I noticed its presence.
Thus, as a PhD researcher in the crop production and horticulture, with a major of plant protection (integrated pest management), I started ask what are the suggested (tested) organic pest control solutions will be me more effective against Nezara viridula ? Kindly I need a cooperation of researchers who read my discussion to share us their research outputs.
Am interested in foliar nutrition practice and irrigation scheduling as main IPM practices used to optimize plant health status. But, other suggested procedures were being welcomed in this survey.
Thanks and Regards
Actually, I am using this software for the analysis of different orders of insect with seven different soil parameters and want to draw a biplot graph to use CCA in PAST, and I am not sure if I am putting my variable correctly or not???
Need help in this regard?
What is the primary role of the staphylococcus bacteria species in insect groups?
Mainly, I wonder relation with the bee species.
Thank you to those who showed interest and posted comments and replies.
The stomach content belong to Lepomis gibossus also known by the pumpkimseed sunfish who lives in fresh water . So please I want to know the ID of the this diptera ( family, genus... ).
I am trying to determine the population structure of a dipteran insect. Most of the previous studies have performed the studies using SSRs. However I want to know if the same studies can be performed using ITS sequences. If so what are the expected differences that might arise?
Hello, I am currently analyzing data on insect counts. I am comparing insect orders (counts) across four seasons and three elevations collected using three different methods. My primary question is "How do insect order counts vary with season and elevation?" I am using 'glmmadmb' function in R to fit a model. I came across so many combinations and tried to choose the best model based on AIC, BIC, overdispersion, and log-likelihood values. However, I am confused if the model indicated by these parameters is appropriate or not. For e.g. the best model indicated is:
mod <- glmmadmb(counts ~ season+(elevation|order), family="nbinom", data =x)
But I believe mod1 should be used:
mod1 <- glmmadmb(counts ~ season+elevation+(1|order), family="nbinom", data =x)
Further, what if I want to incorporate the variable "method of the collection" into the model? Would it be something like this?
mod3 <- glmmadmb(counts ~ season+elevation+(1|order)+(1|method), family="nbinom", data =x) or even more complicated?
After reading so many papers, I am confused about the various combinations of random effects like e|g; (1|e)+(1|g); (1|e/g); (0|e/g) etc.
I have spent days trying to figure this out. The more I read, the more confused I am. Any help would be highly appreciated.
I have been given 4 organisms (insect) and need to manually construct the max possible trees and then choose the most parsimonious and back this up by research. they are arthropods.
first How can i verify what are the number of possible trees, I have already drawn 12, but got feedback that that is not enough. I am using 10 characters.
I want to study the life cycle of insect, the larva of this insect lives inside the gall which is formed in the stem of host plant. I presume the presence the larva leads to the formation of a gall. Can someone suggest me a standard protocol through which I can study the life cycle of this insect larva.
Thank you in advance
Dr Bisu Singh
Trying to determine the metabolites responsible for cowpea due insect resistance against aphids (A. craccivora) and weevils (C. maculatus).
Can anybody recommend an adjuvant/superfactant that could be combined with the insect growth inhibitor Cyromazine to increase absorbency and efficacy. Ideally, we are looking for a product that is widely available. Thanks!
If organic insect-aware farming were to be implemented completely, assume bad harvests such as the potato famine would be around the corner at some stage.
What is the tradeoff of organic farming vs short-term stable pesticide farming with potential disruptive biodiversity loss in the insect population in the long term?
Cherish your research and/or qualitative opinion.
Can Beauveria bassiana control Guvava Bark borer; (Indarbella tetraonis)? If anyone has ethentic recommendation about this insect please share.
Can Beauveria bassiana control Guvava Bark borer; (Indarbella tetraonis)?
If anyone has ethentic recommendation about this insect please share.
Recently, i have started working with SF9 cell line from thermo (Cat No. B82501) for which i was able to culture and grow the newly received vial in complete Grace's insect media at 27 degree Celsius in a non-humidified incubator as per the instructions (bright field images attached for reference). But i am not able to witness the viable cells after revival of frozen cells from previous batch. Approximately 10*7 cells/ml cells were frozen with 80% complete Grace's insect media, 10% heat inactivated FBS, and 10% DMSO as per instructions. Even though the doubling time is 24-30 hrs, there is slow growth of cells even after 7days.
Seeking expert advice regarding the possible reason for such slow growth of cells and how to enhance the culture conditions.
Cameron (2014) reports that the mitogenomes of 28 insect orders have been sequenced so far. Is there a recent publication that presents similar data for different taxonomic levels?
My research topic is to explore the biogeograpgic patterns of species richness of insects. I have the regional richness data of all insects and different orders from many locations. It's well known that insects include c. 30 orders with different numbers of species and phylogenies. I want to group different insect orders into several groups, and make a clear description of their diversity patterns. The problem is in grouping different insect orders into several groups.
I'm also looking for someone interested in this project. Please contact me if you want to join me.
I am involved in a project on biological control of the Comstock mealybug Pseudococcus comstocki in Switzerland. As part of this project, we are doing host specificity tests of a parasitoid and, besides P. comstocki, have tested so far the following non-target species: Pseudococcus longispinus, Planococcus citri and Phenacoccus aceris. We would like to test more species of the family Pseudococcidae and are looking for someone in Europe who could give us an identified starting colony for this purpose.
Thank you for your help!
I am working on my Tesis, and I need papers related with morphology and morphometry of cockroaches or any related insect (opthopteroids).
We purchased a new CG-MS and the provider suggested to use intrastent grade helium (to be cleaned with filters) instead of analytical grade He. I have always used analytical grade before and I am unsure of the impact of the swap on the quality of my samples (I mostly analyse plant and insect volatile compounds, some of which are present in minute amounts). I would be thankful for any technical advice you can provide.
With kind regards,
I recently deployed some insect pitfall traps and in several of them I unfortunately caught mice. How can I avoid this? I have seen suggestions of using shallow traps or small ladders, what is the best way to obtain quality insect samples and minimize risk to small mammals?
I have recently deployed some insect pit traps in the field, but I am concerned they may flood and overflow during rainstorms. Does anyone have recommendations for solving this problem?
This insect inclusion is sitting a Cretaceous amber from the Carpathian Basin. I am looking for ideas on what this insect could be.
The dorsal side (?wings) might bear some scale-like structures. Any ideas are welcome!
Hi, I am looking for help emulating the process depicted in the image below. Essentially I am looking for a method to extract the juices out of feeder insects such as crickets, roaches, mealworms, etc in large quantities that ideally doesn't require any costly tools, without altering the juice in any physical (heating/desiccating) or chemical way. In the image below it was done without the use of a centrifuge, so I would like to avoid needing to purchase one. I'm aware that there are procedures for extracting hemolymph alone, but I need all liquid contents of the insects, not just the hemolymph. I tried using a juicer machine but all that did was sadly turn the insects into a paste. Any advice is more than appreciated, thanks!
I want to do research on some insects organs association of functional morphology, there I want steps and procedure to make research. Please help me. Here I have attached pictures for reference
Would the dominance of one of the microbes in the gut change if we give different foods to insects or larvae? Suppose the insect is supplemented with amylase-producing bacteria, fed with carbohydrates such as rice, corn, and other sources.
We are running qPCR experiments with cDNA samples derived from insect tissues using a QuantStudio Real-Time-PCR-System from Applied biosystems. Since a few weeks, we are facing severe problems with S-shaped or sigmoid amplification curves, leading to extremely decreased CT values for the relevant PCR samples. Intriguingly, comparing two technical replicates running in two adjacent wells, one sample is associated with this problem while the other one is normal. This is demonstrated in the attached figure that shows the amplification curves of two technical replicates: the one on the right hand is normal whereas the other is sigmoid.
The problem is not linked to a specific primer pair since it occurs with different primer pairs that have been used in the past without any problems.
Does anyone have a good idea how to avoid this problem in the future?
The targeted insects include fall armyworm and locusta migratoria. Need proteins study for further information. Suggest me proteins, database, or any research paper. Just share the specific insects proteins which are not involved in humans.
Hello insect cell experts,
I am currently culturing Sf9 insect cells for a protein expression and noticed some tadpole-like cells (red circled in the picture). They seem to reduce/disappear when cells are close to confluence. Are they just unhappy Sf9 cells, or could it be a contamination?
These cells are recovered from a cryo-stock and not yet transfected.
For the culturing, I use supplemented Grace's medium with 10% FBS and Pen/Strep.
I would appreciate if anyone with insect cell experience could tell what they are.
Thanks in advance!
I need some transparent medium for mounting small insects. I need a medium that a bit dissolves soft tissues and not needs complicated chemical procedure with mounting.
I am looking for a lab that can process insect DNA samples for me. More precisely, I would like to do hyRAD on my samples (they are not good enough for ddRAD). I am struggling to find one.
I am currently conducting an experimental study. I don't have any methods on how to count the generations that might be exhibited by the insect that I mass reared.
In our case it is impossible to traced from where is originated (species is clearly different from all others and belongs to group with limited distribution in Middle East).
I'm trying to get some good microscopic photographs of insect genitalia for my research work but I'm having trouble removing the air bubbles that comes inside the genitalia, no matter how cautious I'm.
Suggestions are appreciated.
I found this insect in the digestive tract content of Hypoatherina temminckii (marine fish). The sampling location in seagrass waters of Karang Congkak Island, Kepulauan Seribu (Seribu Island), Indonesia. I can't identify the insect groups, I just suppose this is part of Diptera but have never seen marine (or semi-aquatic) insects in my sampling location.
I'm trying to know where the holotype of Psammotettix confinis (described by Dahlbom in 1850) is conserved.
I searched informations on GBIF, INPN, EOL and internet, unfortunately, I didn't find anything.
Can you help me to know how can I find where the holotype is conserved?
I'd like to call your attention to browse and read a recent collection of papers on insect extinction, just published in Ecological Entomology: https://onlinelibrary.wiley.com/doi/toc/10.1111/(ISSN)1365-2311.insect-extinctions
I want to assess the similarity of insect communities within and between two groups of plants: a monophyletic clade and a paraphyletic grade.
Would you recommend calculating Bray-Curtis dissimilarities + Kruskal Wallis H test or a multivariate test such as MRPP or PERMANOVA?
Thank you for your help
How long will it take for arthropod ingredients to appear on our menu?
Recently, Nestle has released food for dogs and cats, in which, in addition to the usual chicken, they added chopped fly larvae. And no, the global corporation does not save on cats. Livestock is one of the drivers of climate change, and replacing cows with insects can reduce its turnover. Some insect products have been on the market for a long time. Tell us who you can try and what sensations to expect?
I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification.
I may need to keep the samples stored for up to two months and they also need to "survive" an international trip.