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Insect - Science topic

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I am seeking clarification on the differences between the two commercial baculovirus systems for insect expression: Bac-to-Bac system (Invitrogen)and the MultiBac system (from by Imre Berger and colleagues) for insect expression.
It has come to my attention that specific plasmids, such as pACEBac1/pFBDM for MultiBac and pFastBac/pFastBacDual for Bac-to-Bac, are associated exclusively with their respective systems. Would someone be able to confirm whether these plasmids are indeed non-interchangeable between the two systems and, if so, an explanation for this distinction. The plasmids look similar, so is there a difference in the mechanism?
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Only main difference between the two systems is that the MultiBac system allows for construct assembly using CRE/Lox recombination before Tn7 transposition. The plasmids should be interchangeable.
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Which radars do you recommend? Manufacturers?
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I have no any study about it.
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Hello, I have encountered a lot of these unusual structures while looking for Middle Triassic conodonts. The sample was dissolved in formic acid and has yielded conodonts, agglutinated foraminifera and fish teeth.
Could these perhaps be pieces of a sponge skeleton, or are they more likely pieces of plastic foam or something similar? Fused sponge skeletons seem to have more rounded edges, an these seem to have pronounced angles. Sponges also seem to rarely form pentagonal meshes...
I washed the sample pieces before dissolving them, but I wouldn't be surprised something got stuck in the cracks and nooks (e.g., there was a very small insect leg part in one of the fractions). I tested if these structures sink in water; they do, but very slowly. I am aware that some plastic does, however, sink in water.
Could anyone more experienced with micropaleontology help?
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these are probably no plant parts, including no phytolithes
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I am trying to extract DNA from a single insect from different sites. After extraction nanodrop readings show around 12-15 nanogram per microlitre concentration which is normal for the insect. However PCR amplification from two specific sites are failing everytime inspite of DNA being present. I have checked for presence of inhibitors by extracting DNA from four sites simultaneously which included the two sites mentioned above. DNA concentration is sufficient. However PCR amplification failed again. What could be the reasons for the failing PCRs?
I am attaching the gel image of the samples.
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The PCR result is influenced and inhibited by substances used in DNA isolation. You should pay attention to the concentration of the substances you are using and bring them to the appropriate concentration.
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Is it possible to keep them in -80 freezer or should we keep them in liquid nitrogen?
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According to this paper, they can be stored for 1 year at -80 and retain the same viability as liquid nitrogen storage. After 1 year there is small decrease in viability.
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I recently encounter a devastating insect. I want to know more to control it. Any one familiar with the insect pest?
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Xhantogaleruca luteola is ok.
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Greetings ResearchGate Community,
I was wondering if someone could kindly shed some light on the identity of the red insect in this photo? This appears to be a species of katydid but I have never seen anything like it before. Any information will be greatly appreciated.
Thank you all so much!
Jack
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Panoploscelis species. Is this from China Jack
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Can we alter the genome of a polyphagous plant insect pest to a monophagous and phytophagous insect to insectivorus? Is there any example? Please share your thoughts..
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Insects have various clusters of chemical receptors around their mouths (best studied in silkworms). If you were to alter which receptors were where you could change what the insect would try to feed on.
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How can I investigate plastic-vorous Galleria Mellonella's 10th generation based on cellular, molecular and environmental aspects?
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Dear Ali, I recommend you be more specific in your questions. Are you looking for a methodology to help you study Galleria mellonella at the cellular and molecular levels? Or do you need help designing an experiment including feeding plastic and sampling the 10th generation of wax moths?
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Dear coleagues,
could anybody ID a leaf miner? I haven't imago picture, only larva, pre-pupa, and pupa.
Host plant is Platanus. West of Ukraine, 03.07.2023
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what about the Lepidoptere Phyllonorycter platani?
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Please I need your response on this.
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There are different types of life tables and different terms are used. Many life tables do not include reproduction...they might just follow a single generation through time. But when they do ... mx is another (generally older) term for fx, both are usually defined as the stage or age- specific fecundity. Normally if you can rear the insect then determine the average no. of eggs oviposited per female over their life span. In that case fx (or mx) would equal the fecundity over that full life stage. You could record eggs produced for each day, as was done for Medfly for example, such that f1 = eggs produced on day 1, f2= eggs produced on day 2, etc... mx was the original term for egg production or fecundity. fx is used in matrix models and in more recent life tables that can be readily modeled using matrix methods.
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Any suggestions and refrence would be very helpful . Most probably I think psocid but ant idea up to species level or genus level
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This mite is the larva of a terrestrial Parasitengona. Most likely a Leptus from its flask-shaped gnathosoma, although that's somewhat speculative (it's a little hard to see for sure). The terrestrial Parasitengona is very diverse and still has many undescribed species. Almost all of them are parasites as larvae. This one is probably engorged and has dropped off its host unless, of course, you removed it from a host arthropod.
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Do apricot tree blossoms need insect pollination? Especially the items in Algeria?
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Some varieties of apricot are self-incompatible whereas others are self-compatible.
See paper below in Journal of Horticultural Science:
J. Rodrigo & M. Herrero: Evaluation of pollination as the cause of erratic fruit set in apricot ‘Moniqui’ https://doi.org/10.1080/14620316.1996.11515461
However, I believe that in both cases insect pollination increases the chances of fruit set. Beneficial effect of insect pollination can be higher in case of self-incompatible varieties.
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What are the methodologies involved in sequencing and aligning the genome of an unknown species of insect for the purpose of constructing a phylogenetic tree? Are there any institutions or facilities that offer sequencing services specifically tailored for unknown insect species? What are the recommended protocols in order to initiate the sequencing process?
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Depends if you want to use long or short reads. There are facilities like the Sanger Institute that do the sequencing. As for the assembly of the reference genome, there are pipelines (eg. ). Usually you need about 3 micrograms of high quality DNA. Your model being an insect should not change anything once you have performed the DNA extraction step. Maybe ask around your own university or nearby ones if any of the labs have an Illumina sequencing platform.
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I have been trying to compare the efficiency, cost-effectiveness, and convenience of the Piggybac and Bacmid systems, both of which have been utilized extensively in insect genetics. However, I have not been able to find a conclusive answer. If someone has worked extensively in this area, I am hoping to have some insights that could help me understand the pros and cons of each system better.
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Technically you can use transposases from other sources but it might have a lower transposition efficiency. The restricted use is purely because of IP so you should check which countries they have their patents enforced in. The lack of the ARS is probably to stop the plasmid replicating in transfected cells so it is quickly lost after transfection. The documentation for the plasmid indicates it has a conditional origin of replication.
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Hi
i am starting working with insect cell protein expression from previously working with bacterial protein expression. for bacteria you can pellet and then freeze the cells after expression (and sometimes it even helps the lysis later).
I am gonna express a protein via Baculovirus in insect cells can i harvest and freeze the pellet before protein extraction and purification?
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This should work ok, but I'd include an insect specific protease inhibitor cocktail and then snap freeze fast, maybe with LN2. Controls are also , of course, a very good idea! Good Luck!
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It's known that phytates are a P storage in plants, in particular seeds. Plants produce enzymes to release P upon need. Also ruminants can digest phytates thanks to their gut microbiome. However, for many animals phytic acid is an antinutrient because it binds nutrients in cationic form (Ca2+, Mg2+ etc.). Insect have proven able to digest a wide variety of substrates thanks to their gut microbiome. I found mentions of phytates in insect-based products, but it wasn't addressed whether insects can digest them. Do you know if any experiment proved they can digest phytates?
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Dear Dr.Nicolò M. Villa,
you can search about the ability of Black solider fly to digest such substrate "phytic acid" which plants enriched with such acid, as I know Black solider fly larvae their gut microbiome has the ability to digest such substrate.
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This insect is an interesting major pest on tomatoes, peppers, beans, peas, corn and soybeans. During my research travels, I found it as a common insect in variegated geographical regions worldwide. Florida in USA and Debrecen in central Europe regions are two main different areas from geographical, and ecological standpoints where I noticed its presence.
Thus, as a PhD researcher in the crop production and horticulture, with a major of plant protection (integrated pest management), I started ask what are the suggested (tested) organic pest control solutions will be me more effective against Nezara viridula ? Kindly I need a cooperation of researchers who read my discussion to share us their research outputs.
Am interested in foliar nutrition practice and irrigation scheduling as main IPM practices used to optimize plant health status. But, other suggested procedures were being welcomed in this survey.
Thanks and Regards
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Eocanthecona furcellata is predator and Stink Bug (Nezara viridula) or Southern green Stink Bug is pest and they are they all are Pentatomid bugs.
We can easily recognize with their appearances.
Eocanthecona furcellata eggs are golden and they turn red when they nearly hatch.
Nezara viridula eggs are white
Eocanthecona Furcellata egg
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Nezara viridula egg
The rostrum of Eocanthecona furcellata adult and nymph are bigger than their antenna
The rostrum of Nezara viridula adult and nymph are the same as their antenna
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Actually, I am using this software for the analysis of different orders of insect with seven different soil parameters and want to draw a biplot graph to use CCA in PAST, and I am not sure if I am putting my variable correctly or not???
Need help in this regard?
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Dear Priya,
CCA (Canonical Correspondence Analysis) is a powerful multivariate technique that can be used to investigate the relationship between species (in this case, different orders of insects) and environmental variables (in this case, soil physical-chemical parameters). PAST (Paleontological Statistics) is a software package that can perform CCA and other multivariate analyses.
To use CCA in PAST, you must organize your data in a specific format. It would be best to have a matrix or data frame with the insect order (species) as rows and the soil physical-chemical parameters as columns. The values in the matrix should be the abundance or occurrence of each insect order in each soil sample.
You should also ensure that the data is adequately transformed to meet the assumptions of CCA. For example, you should use a log transformation if the data is skewed. Also, you should ensure the data has been standardized (mean = 0, standard deviation = 1) before performing the analysis.
Once your data is in the appropriate format, you can use PAST to perform the CCA analysis.
To draw a biplot graph in PAST, use the "Ordination" option, then select "CCA." Once the analysis is completed, you can use the "Graph" option to create the biplot graph. The biplot graph will show the relationship between the insect orders and the soil physical-chemical parameters on the ordination space.
It's important to note that CCA assumes that the environmental variables (soil physical-chemical parameters) are independent, so if there is a correlation between the variables, it's better to use PCA (Principal component analysis) or RDA (Redundancy analysis) before running the CCA.
It's also essential to make sure that the data meet the assumptions of the CCA, such as linearity and normality, and the data should be transformed if the premise is unmet.
It's also essential to consult with experts in the field or consult the literature to have more information about the recent developments in the area and also the interpretation of the results.
I hope I was able to answer your question.
Yours sincerely,
Edgar M Cambaza
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This insect is from Sana'a, Yemen. It is nocturnal from the family Curculionidae. One of my colleagues want to study this species, but he did not know the species or the genus of this insect. Could anyone help?
Kind regards
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Most probably Vietomorpha cf. foveipenne (Fairmaire). Tenebrionidae Spediini Salem Busais
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What is the primary role of the staphylococcus bacteria species in insect groups?
Mainly, I wonder relation with the bee species.
Thank you to those who showed interest and posted comments and replies.
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Thank you so much, dear Geis.
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The stomach content belong to Lepomis gibossus also known by the pumpkimseed sunfish who lives in fresh water . So please I want to know the ID of the this diptera ( family, genus... ).
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It is not possible to accurately identify the family, genus, or species of a diptera (a group of insects including flies, mosquitoes, and midges) based on the stomach content of a Lepomis gibbosus (pumpkinseed sunfish). In order to accurately identify a diptera, it is necessary to examine the physical characteristics of the insect, such as its size, shape, color, and specific morphological features.
To identify a diptera, you will need to collect a specimen and observe it using a microscope or other specialized equipment. You can then compare the characteristics of the insect to those of known diptera species in order to determine its identity. Alternatively, you can consult a taxonomic reference or seek the assistance of a diptera expert who has the knowledge and expertise to identify the insect.
I hope this information is helpful! Let me know if you have any further questions.
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I am trying to determine the population structure of a dipteran insect. Most of the previous studies have performed the studies using SSRs. However I want to know if the same studies can be performed using ITS sequences. If so what are the expected differences that might arise?
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Yes, population structure analysis can be performed using internal transcribed spacers (ITS) instead of SSRs. ITS are regions of non-coding DNA between the 16S and 23S ribosomal RNA genes. They are commonly used in population genetics studies because they can be sequenced quickly and provide a good measure of genetic differentiation between populations.
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The cubital index is a measure of the relative length of the cubital vein, which is a vein located in the forewing of a honeybee. The cubital index is calculated by dividing the length of the cubital vein by the length of the wing and expressing the result as a percentage.
The cubital index is used as a morphological character in the identification and classification of honeybee subspecies and populations. It has been found that the cubital index can vary significantly among different honeybee subspecies and populations, with some subspecies having a relatively long cubital vein and others having a relatively short one.
There is some evidence to suggest that the cubital index may be related to the foraging behavior of honeybees. Some studies have found that honeybees with a higher cubital index may be more efficient at foraging and more successful at finding food resources, while those with a lower cubital index may be less efficient at foraging.
In addition to its use in the identification and classification of honeybees, the cubital index may also have practical applications in the management of honeybee colonies. For example, some beekeepers may use the cubital index as a tool for selecting bees with desirable foraging traits for breeding purposes.
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Hello, I am currently analyzing data on insect counts. I am comparing insect orders (counts) across four seasons and three elevations collected using three different methods. My primary question is "How do insect order counts vary with season and elevation?" I am using 'glmmadmb' function in R to fit a model. I came across so many combinations and tried to choose the best model based on AIC, BIC, overdispersion, and log-likelihood values. However, I am confused if the model indicated by these parameters is appropriate or not. For e.g. the best model indicated is:
mod <- glmmadmb(counts ~ season+(elevation|order), family="nbinom", data =x)
But I believe mod1 should be used:
mod1 <- glmmadmb(counts ~ season+elevation+(1|order), family="nbinom", data =x)
Further, what if I want to incorporate the variable "method of the collection" into the model? Would it be something like this?
mod3 <- glmmadmb(counts ~ season+elevation+(1|order)+(1|method), family="nbinom", data =x) or even more complicated?
After reading so many papers, I am confused about the various combinations of random effects like e|g; (1|e)+(1|g); (1|e/g); (0|e/g) etc.
I have spent days trying to figure this out. The more I read, the more confused I am. Any help would be highly appreciated.
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You are using count data so I suggest that you read the attached R information. I'm also attaching a paper of ours that uses similar methods for binomial data that may be of some help to you. If you have questions please ask. Best wishes David Booth
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I have been given 4 organisms (insect) and need to manually construct the max possible trees and then choose the most parsimonious and back this up by research. they are arthropods.
first How can i verify what are the number of possible trees, I have already drawn 12, but got feedback that that is not enough. I am using 10 characters.
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So you are short three trees. You must have another three ways to root the tree.
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I am wondering what species of insect this is?
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iTs a fly diptera
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I want to study the life cycle of insect, the larva of this insect lives inside the gall which is formed in the stem of host plant. I presume the presence the larva leads to the formation of a gall. Can someone suggest me a standard protocol through which I can study the life cycle of this insect larva.
Thank you in advance
Dr Bisu Singh
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Thank you James and Firas for you suggestions.
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Trying to determine the metabolites responsible for cowpea due insect resistance against aphids (A. craccivora) and weevils (C. maculatus).
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Do you have access to gas/ liquid chromatographer and mass spectrometer? If yes, you need to extract the leaves and seeds in a HP(polar for HPLC and non-polar for GC) and run the extract on HPLC-MS or GC-MS. Terpenoids are mostly non-polar so GC and GC-MS is probably a better bet for you than HPLC.
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Can anybody recommend an adjuvant/superfactant that could be combined with the insect growth inhibitor Cyromazine to increase absorbency and efficacy. Ideally, we are looking for a product that is widely available. Thanks!
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While I do not have a specific product to recommend, the company Exacto, located in the Chicago area is adept at creating and formulating surfactants and adjuvants. They may even have a branded product in the market that would be perfect for your needs.
Contact info below:
Glen Obear, Ph.D.
RDI Director | Exacto®, Inc.
P:+1-920-287-8117
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Hi frds,
If organic insect-aware farming were to be implemented completely, assume bad harvests such as the potato famine would be around the corner at some stage.
What is the tradeoff of organic farming vs short-term stable pesticide farming with potential disruptive biodiversity loss in the insect population in the long term?
Cherish your research and/or qualitative opinion.
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The idea that organic farming represents a low yield and solution is erred.
The Rodale Institute Farming Systems Trial show that comparative yield using strictly organic biological based inputs is completely competitve to agrichemical reliance.
As furth example I would take the case of potato farming which has enormous ability to provide nutrition directly to consumers. Commercial potato farmers have become major users of compost in order to increase both the yield and quality of potatoes.
The hungry potato crop responds very positively to compost and rotation which are core organic practices allowing both high yields and reduced use of pesticides.
The idea that pesticides and fertilizers guarantee high yield and stable production is flawed.
After a 3-year transition to organic agriculture the Rodale Institute has shown there is no significant reduction in yield and in years of drought the organic systems improve yields over conventional agriculture.
The reliance of solely the use of agrichemical inputs increases farmer costs but biological inputs can avoid their side effects and lead to high more stable production of maize, soybean and wheat which are major field crops in North America.
The use of organic agriculture techniques reduces the carbon foot footprint of the production systems and the ability to increase soil carbon and nitrogen not only counteracts need for synthetic inputs but also contribute to counterbalancing the enrichment of atmospheric greenhouse gases,
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Hello all:
Could anyone tell me, where to get the research articles about evaluated insect natural enemies Ecological value.
Thanks.
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You can get your answer in this article
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Is it a sensory setae .? If so how it can be differentiate from other such setae or sensillae.?
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Trichobothria are very long, fine hair-like setae, that probably respond to air movement. They can be found on the antennae in most species, and on the nota of Lepismatidae where the arrangement of the trichobothrial areas is taxonomically useful (see work of Luis Mendes)
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I want to research the effect of black aphids inside the plant
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Black bean aphid is the sucking pest which sucks the cell sap from the plant parts resulting yellowing, curling, wilting, and finally death of the entire plant. Similarly they also aids to transmit viral disease which results change in the texture of the plant and its part.
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Other than the dead heart?
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The larvae attack the plant, especially the bases of the leaves, and burrow into them, which leads to yellowing of the plant, pallor and poor production. Severe infestation leads to permanent wilting, and the plant can be easily uprooted and an unpleasant odor appears as a result of rotting.
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Can Beauveria bassiana control Guvava Bark borer; (Indarbella tetraonis)? If anyone has ethentic recommendation about this insect please share.
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Now a days, it's a global organic movement. Organic crops, organic food etc.etc. There is plenty of scope for organic culture. It's less toxic than synthetic/pure agrochemicals. Organic agrochemicals (pesticides/ fertilizers) are the byproducts of the living organisms and metabolizes very easily with less side effects. As far as market is concerned, sky is the limit. Organic does not mean genetically modified. Not at all. GM food/ crop have lots of delayed side effects may be in next or next to next generation. Every time I have used the word less toxic, because nothing is safe. There is no word "SAFE" in toxicology.
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I am working on insect identification.Can any one suggest me from where I can find data set for images of rice crop insects.
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hey did you find any dataset about insects on rice plants
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Can Beauveria bassiana control Guvava Bark borer; (Indarbella tetraonis)?
If anyone has ethentic recommendation about this insect please share.
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Yes, of course you can use the Beauveria bassiana to control Guvava Bark borer.
-The B. bassiana parasitizes a very wide range of arthropod hosts (infecting different types of invertebrates: insects: Thrips, White fly,Termites, Stem borers, Beetles, Guava Bark borer, Colorado potato beetle, Moth, Corn borer, Caterpillars, Root weevil etc.).
- B. bassiana can be very virulent when applied in optimal conditions, using the right mixing and application techniques. for example, apply B. bassiana in the late afternoon, in the evening or on a rainy day. For best results in applying B. bassiana : you need applying early when the larvae first stage, also used as prevention rather than a cure (apply where you see pests directly), but B. bassiana spores are very sensitive to UV rays.
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Hello everyone!
Recently, i have started working with SF9 cell line from thermo (Cat No. B82501) for which i was able to culture and grow the newly received vial in complete Grace's insect media at 27 degree Celsius in a non-humidified incubator as per the instructions (bright field images attached for reference). But i am not able to witness the viable cells after revival of frozen cells from previous batch. Approximately 10*7 cells/ml cells were frozen with 80% complete Grace's insect media, 10% heat inactivated FBS, and 10% DMSO as per instructions. Even though the doubling time is 24-30 hrs, there is slow growth of cells even after 7days.
Seeking expert advice regarding the possible reason for such slow growth of cells and how to enhance the culture conditions.
Thanks
Kanan
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Wen Liu is it required to use medium supplemented with 20% FBS when subculturing the culture or we use it only in the first step?
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Cameron (2014) reports that the mitogenomes of 28 insect orders have been sequenced so far. Is there a recent publication that presents similar data for different taxonomic levels?
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Agreed with Dong. Just search in NCBI and count it.
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    My research topic is to explore the biogeograpgic patterns of species richness of insects. I have the regional richness data of all insects and different orders from many locations. It's well known that insects include c. 30 orders with different numbers of species and phylogenies. I want to group different insect orders into several groups, and make a clear description of their diversity patterns. The problem is in grouping different insect orders into several groups.
    I'm also looking for someone interested in this project. Please contact me if you want to join me. 
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By taxonomic characters for example the order of Lepidoptera includes butterflies
The diptera order include flies
The hymenoptera order includes bees, wasps, hornets and ants
Coleoptera includes bettles
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I am involved in a project on biological control of the Comstock mealybug Pseudococcus comstocki in Switzerland. As part of this project, we are doing host specificity tests of a parasitoid and, besides P. comstocki, have tested so far the following non-target species: Pseudococcus longispinus, Planococcus citri and Phenacoccus aceris. We would like to test more species of the family Pseudococcidae and are looking for someone in Europe who could give us an identified starting colony for this purpose.
Thank you for your help!
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Dear Jinan, thanks for your answer, that is good to know! Which species are those? Just in general whatever is attacking ornamental plants? Are there some dominant species? Greetings, Lukas
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I am working on my Tesis, and I need papers related with morphology and morphometry of cockroaches or any related insect (opthopteroids).
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thank you!
Best wishes
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We purchased a new CG-MS and the provider suggested to use intrastent grade helium (to be cleaned with filters) instead of analytical grade He. I have always used analytical grade before and I am unsure of the impact of the swap on the quality of my samples (I mostly analyse plant and insect volatile compounds, some of which are present in minute amounts). I would be thankful for any technical advice you can provide.
With kind regards,
Andrea
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As friends and professors have said, one of the best ways is to use alternative gas, where hydrogen and nitrogen can be the best choices.These articles can help you in this way
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Being from the same field of insect breeding, can I have a video of this project?
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I recently deployed some insect pitfall traps and in several of them I unfortunately caught mice. How can I avoid this? I have seen suggestions of using shallow traps or small ladders, what is the best way to obtain quality insect samples and minimize risk to small mammals?
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Great question. I have gone through the same issue while laying baited pitfall traps and have sadly captured reptiles in the trap, along with my study group of beetles. Please do checkout the discussion thread here for some ideas : https://www.researchgate.net/post/How-to-prevent-that-small-vertebrata-get-caught-in-regular-soil-pitfall-traps
I would also recommend you to go through the methodology followed by where their intent was to capture small vertebrates but exclude larger predators like mammals? Might be useful.
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I have recently deployed some insect pit traps in the field, but I am concerned they may flood and overflow during rainstorms. Does anyone have recommendations for solving this problem?
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If the container is made of plastic, drill a small hole in the the upper part such that the liquid will flow out of the container but the insect will be retained. Alternatively, install a "roof" (we used painted plywood) such that the rain will be deflected and will not enter the container. The roof will also prevent the liquid to evaporate quickly.
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Dear Everyone!
This insect inclusion is sitting a Cretaceous amber from the Carpathian Basin. I am looking for ideas on what this insect could be.
The dorsal side (?wings) might bear some scale-like structures. Any ideas are welcome!
Sincerely;
Márton
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Hi Márton,
It looks very similar to Alienopterix smidovae Hinkelman, 2021, this genus is tentatively included in Umenocoleidae, a enigmatic family within Dictyoptera.
Further reading:
Vršanský P, Sendi H, Hinkelman J, Hain M. 2021. Alienopterix Mlynský et al., 2018 complex in North Myanmar amber supports Umenocoleoidea/ae status. Biologia
Luo C, Beutel RG, Engel MS, Liang K, Li L, et al. 2022. Life history and evolution of the enigmatic Cretaceous–Eocene Alienopteridae: A critical review. Earth-Science Reviews 225: 103914
Luo C-H, Beutel RG, Thomson UR, Zheng D-R, Li J-H, et al. 2021. Beetle or roach: systematic position of the enigmatic Umenocoleidae based on new material from Zhonggou Formation in Jiuquan, Northwest China, and a morphocladistic analysis. Palaeoworld 31: 121–30
Hope it helps.
Best wishes,
Cihang
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Hi, I am looking for help emulating the process depicted in the image below. Essentially I am looking for a method to extract the juices out of feeder insects such as crickets, roaches, mealworms, etc in large quantities that ideally doesn't require any costly tools, without altering the juice in any physical (heating/desiccating) or chemical way. In the image below it was done without the use of a centrifuge, so I would like to avoid needing to purchase one. I'm aware that there are procedures for extracting hemolymph alone, but I need all liquid contents of the insects, not just the hemolymph. I tried using a juicer machine but all that did was sadly turn the insects into a paste. Any advice is more than appreciated, thanks!
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I want to do research on some insects organs association of functional morphology, there I want steps and procedure to make research. Please help me. Here I have attached pictures for reference
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Dear Babu Kes
The software product will suit you Aidos-X. It is written for entomologists. Many articles are devoted to ground beetles. If you have any questions write.
Regards, Sergey
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Would the dominance of one of the microbes in the gut change if we give different foods to insects or larvae? Suppose the insect is supplemented with amylase-producing bacteria, fed with carbohydrates such as rice, corn, and other sources.
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Kindly check the following RG link in which a representative strain collection of dominant aerobic bacteria from black soldier fly larvae (Hermetia illucens, BSFL) has been established:
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We are running qPCR experiments with cDNA samples derived from insect tissues using a QuantStudio Real-Time-PCR-System from Applied biosystems. Since a few weeks, we are facing severe problems with S-shaped or sigmoid amplification curves, leading to extremely decreased CT values for the relevant PCR samples. Intriguingly, comparing two technical replicates running in two adjacent wells, one sample is associated with this problem while the other one is normal. This is demonstrated in the attached figure that shows the amplification curves of two technical replicates: the one on the right hand is normal whereas the other is sigmoid.
The problem is not linked to a specific primer pair since it occurs with different primer pairs that have been used in the past without any problems.
Does anyone have a good idea how to avoid this problem in the future?
Best regards,
Joerg
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The problem comes from difficulties of the software to recognize the correct exponential phase. This point needs to be estimated to determine the range of the baseline fluorescence, which is subtracted from the curve. In your case, the software seems to wrongly interpret some early signal increase as he exponential phase. You can solve this problem by adjusting the settings for background correction. You should at least be able to manually define the range of cycles from which the baseline fluorescence should be estimated (should be about 3-19 or 12-19 to exclude the "problematic" region in the early cycles).
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The targeted insects include fall armyworm and locusta migratoria. Need proteins study for further information. Suggest me proteins, database, or any research paper. Just share the specific insects proteins which are not involved in humans.
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For the DNA encoding any locust candidate protein of interest, you can perform a BLASTn search in NCBI to see if any part of it has homology to human genes: Nucleotide BLAST: Search nucleotide databases using a nucleotide query (nih.gov)
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Hello insect cell experts,
I am currently culturing Sf9 insect cells for a protein expression and noticed some tadpole-like cells (red circled in the picture). They seem to reduce/disappear when cells are close to confluence. Are they just unhappy Sf9 cells, or could it be a contamination?
These cells are recovered from a cryo-stock and not yet transfected.
For the culturing, I use supplemented Grace's medium with 10% FBS and Pen/Strep.
I would appreciate if anyone with insect cell experience could tell what they are.
Thanks in advance!
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The elongation and spreading are the properties of normal and healthy cells under tissue culture conditions. Since you have recovered these cells from a cryo-stock and supplemented Grace's medium with 10% FBS and Pen/Strep, the cells of your concern are happily growing in the culture flask. I could see some other cells that are spreading, widening and getting attached to the flask and must be having focal adhesion points on the bottom of the flask. These functions of the cells are markers of normal and healthy culture conditions indicating the availability of sufficient amount of nutrients and growth factors in the culture medium.
One the other hand, if there is shortage of nutrients and growth factors in the culture medium due to large number of cells in confluent conditions, cells feel stressed and then loos spreading-elongation & adhesion properties thats why you do not see these features in cells under confluent conditions. Cells, in order to protect from the stress insult and increase the possibility of survival, they tend to become rounded in order to minimize the surface area and thereby stress insult.
Hence, it is neither contamination not your cells are unhappy. These are the sign of healthy cells getting nutrients and growth factors in the culture medium and are really happy. So be happy.
For more details, you can see our original research publication in Journal of Cellular Biochemistry. The link is given below:
Modulation of a5b1 Integrin Functions by the Phospholipid and Cholesterol Contents of Cell Membranes. .J Cell Biochem 2000 Apr;77(4):517-28.
Best
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I need some transparent medium for mounting small insects. I need a medium that a bit dissolves soft tissues and not needs complicated chemical procedure with mounting.
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Thanks Chen - Digging further, I did get another possible place:
I looked into my ancient records: I got mine from Lonza Inc. It's called DANTOIN 739 there, and a search for that might give you some more paths to follow.
Best wishes, Owen.
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We found cotton fields of Nalgonda District, Telangana State, India
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Rakesh Davella , Oplodontha viridula doesn't occur in India. According to morphology and distribution, this is a female of Oplodontha rubrithorax, as I mentioned above. Please check the following paper:
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I will use it for insect DNA extraction. Can we use sterile distilled water to dissolve?
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Is morphological distinctiveness enough for separating a genus from another?
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Also check please the following useful RG link:
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Hello,
I am looking for a lab that can process insect DNA samples for me. More precisely, I would like to do hyRAD on my samples (they are not good enough for ddRAD). I am struggling to find one.
Thank you!
Sophie
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Tyler Chafin thank you very much !
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I am currently conducting an experimental study. I don't have any methods on how to count the generations that might be exhibited by the insect that I mass reared.
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Number of generation depend on the life cycle start from egg. the time period of one generation starts till the mortality of the insect.It mean one generation include egg, different larval period and adult longevity.
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In our case it is impossible to traced from where is originated (species is clearly different from all others and belongs to group with limited distribution in Middle East).
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Tamara: And if Ruben's suggestions have not helped, describe the species (leaving "type locality unknown") to make other students aware of its existence: maybe it turns out to be already present in some collection but either unidentified or misidentified; or hopefully somebody, knowing what to look for, will find and colect it.
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I'm trying to get some good microscopic photographs of insect genitalia for my research work but I'm having trouble removing the air bubbles that comes inside the genitalia, no matter how cautious I'm.
Suggestions are appreciated.
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No newly described order as such. Why?
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According to the international code of zoological nomenclature names ending with idae is a family name. Earlier Trichogramma was in the family Braconidae. Hence. It is far Way from. Insect order level. It is still in the order Hymenopyeta.
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Is there a written protocol or reference that explains how to isolate the fat body from insect larva (specifically BSF)?
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kindly see:
ORIGINAL RESEARCH article
Front. Insect Sci., 16 June 2021 | https://doi.org/10.3389/finsc.2021.693168
Fat and Happy: Profiling Mosquito Fat Body Lipid Storage and Composition Post-blood Meal
📷Matthew Pinch1*, 📷Soumi Mitra1, 📷Stacy D. Rodriguez1, 📷Yiyi Li2, 📷Yashoda Kandel1, 📷Barry Dungan3, 📷F. Omar Holguin3, 📷Geoffrey M. Attardo4 and 📷Immo A. Hansen1
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I found this insect in the digestive tract content of Hypoatherina temminckii (marine fish). The sampling location in seagrass waters of Karang Congkak Island, Kepulauan Seribu (Seribu Island), Indonesia. I can't identify the insect groups, I just suppose this is part of Diptera but have never seen marine (or semi-aquatic) insects in my sampling location.
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In the upper picture, in my opinion, a diptera from the family Syrphidae
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Hello!
I'm trying to know where the holotype of Psammotettix confinis (described by Dahlbom in 1850) is conserved.
I searched informations on GBIF, INPN, EOL and internet, unfortunately, I didn't find anything.
Can you help me to know how can I find where the holotype is conserved?
Thank you
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Hello everyone!
Thank you very much for all your answers!
I found an answer on EOL (Encyclopedia of Life) : Dahlbom didn't create a holotype when he described the species in 1850.
According to EOL, in 1937, Ossiannilsson decided to create the holotype for Psammotettix confinis (conserved at Carices I Rohne, in Sweden). The type is a male.
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I'd like to call your attention to browse and read a recent collection of papers on insect extinction, just published in Ecological Entomology: https://onlinelibrary.wiley.com/doi/toc/10.1111/(ISSN)1365-2311.insect-extinctions
Thanks
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Thank you for interesting thoughts that will stimulate research on this difficult topic.
Regards, Sergey
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I want to assess the similarity of insect communities within and between two groups of plants: a monophyletic clade and a paraphyletic grade.
Would you recommend calculating Bray-Curtis dissimilarities + Kruskal Wallis H test or a multivariate test such as MRPP or PERMANOVA?
Thank you for your help
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You are most welcome dear
Wish you the best always.
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How long will it take for arthropod ingredients to appear on our menu?
Recently, Nestle has released food for dogs and cats, in which, in addition to the usual chicken, they added chopped fly larvae. And no, the global corporation does not save on cats. Livestock is one of the drivers of climate change, and replacing cows with insects can reduce its turnover. Some insect products have been on the market for a long time. Tell us who you can try and what sensations to expect?
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Depends on the country and culture. Unlikely in Brazil. Most likely in China.
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I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification.
I may need to keep the samples stored for up to two months and they also need to "survive" an international trip.
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Follow the procedure given by Brent & Dolezal, 2009
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Expert comments required to id the insect (Image Attached)
Location : J&K, India
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Dear Amit,
What can be seen from the photo seems to be a dune or desert cricket from the family schizodactylidae, the genus Schizodactylus. They mainly inhabit arid sandy areas.
Best, Elaheh
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Can any one help in the identification of tree hopper found on Guava plant?
Your expertise will be highly appreciated..
Thanks in advance..